Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0022326

Physical Location & Seq

Genomic Coordinates Forward (+)

25634977 .. 25640387

Gene Structure 5411 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1161700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 654 bp

ATGATCATCAATGCTTTCAATTATGGAGCCAATGCTTTCATGGTCAGTATAGCTCATACATTCAGGCAAACCCAAGGGAAGCTAAGATATAGAACTGTGTTTAAGAGGGCAGAGAAGATGGCAGGGATAGAGAGAGGAAGCATCAGAGTAACTGCGCTGATTGAAACACTTCCAGCTGTGTTTCAAATGGATGAGATCCTCTATAAGCTGAGGGACCACTCTGTTTGCTTGAACCGTGGAAGATGGGATTACATTTTCAGCTATACTTACCAAAGGCATGGCGTGAACGTCATGGGTGGCATGGATATGCAACTCTTCGTTCGCATATTGGATCTATATGTTGAATCCGCAGATCGGGTCTTTGAGTCGAGGCCATACGTAGGGTCATCGAGTCGGGTCCGTGAGTCAAGGTTCGAGCTCAAGGGTGTCCAGGCACATAGGTGTCTAAATAAACGAGTGTCCGAGCAAGTGGTTGTCTGGTCAGACAAGAGATCACCGTTCGAACTAAATCTTTGTCACCATGAGCATAGAGAGGAATGTGTGAAGGTTTTCTGGAAAAGCCCAAAGAGTGAGGTTTTTGGGTTCTTGGGTTGTGTAGCTCAGATTCCAATCAGAGATGATCCAAAAGCAAATGAAGCAGCATTGGAGAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004474 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006097 GO:0008150 GO:0008152 GO:0009514 GO:0009987 GO:0016740 GO:0016746 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0046912 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00620 ko00630 ko01100 ko01110 ko01120 ko01200 map00620 map00630 map01100 map01110 map01120 map01200

Pfam Domains

Protein Families

Malate_synthase

Protein Analysis

218

Amino Acids

25.27

Weight (kDa)

9.35

Isoelectric Point (pI)

45.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_TIM-barrel	PF01274	32 - 89	8.9e-23	Malate synthase, TIM barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022326)

Species	Orthologous Gene IDs
malus_domestica	MD14G1161700.v1.1
pyrus_communis	pycom14g13470 pycom72g00080
rosa_laevigata	RLG00000004559

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	348
AclWI	GGATC	3 cut(s)	190, 339, 614
AfiI	CCNNNNNNNGG	2 cut(s)	354, 380
AgsI	TTSAA	5 cut(s)	19, 164, 185, 232, 344
AjnI	CCWGG	1 cut(s)	429
AleI	CACNNNNGTG	1 cut(s)	439
AluBI	AGCT	7 cut(s)	53, 82, 176, 208, 261, 418, 599
AluI	AGCT	7 cut(s)	53, 82, 176, 208, 261, 418, 599
Alw21I	GWGCWC	1 cut(s)	420
AlwI	GGATC	3 cut(s)	190, 339, 614
AoxI	GGCC	1 cut(s)	371
ApeKI	GCWGC	1 cut(s)	638
Asp700I	GAANNNNTTC	2 cut(s)	168, 548
AspLEI	GCGC	1 cut(s)	157
AspS9I	GGNCC	2 cut(s)	214, 397
AsuHPI	GGTGA	2 cut(s)	486, 509
AsuII	TTCGAA	1 cut(s)	501
AvaII	GGWCC	2 cut(s)	214, 397
BanII	GRGCYC	1 cut(s)	420
Bbv12I	GWGCWC	1 cut(s)	420
BbvCI	CCTCAGC	1 cut(s)	209
BbvI	GCAGC	1 cut(s)	650
BccI	CCATC	2 cut(s)	112, 237
BciT130I	CCWGG	1 cut(s)	431
BclI	TGATCA	1 cut(s)	3
BisI	GCNGC	1 cut(s)	639
BlsI	GCNGC	1 cut(s)	640
Bme1390I	CCNGG	1 cut(s)	431
Bme18I	GGWCC	2 cut(s)	214, 397
BmgT120I	GGNCC	2 cut(s)	214, 397
BmiI	GGNNCC	3 cut(s)	28, 215, 398
BmrFI	CCNGG	1 cut(s)	431
BmsI	GCATC	1 cut(s)	150
Bpu10I	CCTNAGC	1 cut(s)	209
Bpu14I	TTCGAA	1 cut(s)	501
BpuEI	CTTGAG	1 cut(s)	404
BsaAI	YACGTR	1 cut(s)	379
BsaBI	GATNNNNATC	1 cut(s)	608
BsaJI	CCNNGG	2 cut(s)	73, 235
Bsc4I	CCNNNNNNNGG	2 cut(s)	354, 380
Bse8I	GATNNNNATC	1 cut(s)	608
BseBI	CCWGG	1 cut(s)	431
BseDI	CCNNGG	2 cut(s)	73, 235
BseGI	GGATG	1 cut(s)	196
BseJI	GATNNNNATC	1 cut(s)	608
BseLI	CCNNNNNNNGG	2 cut(s)	354, 380
BseMII	CTCAG	2 cut(s)	200, 614
BseXI	GCAGC	1 cut(s)	650
BshFI	GGCC	1 cut(s)	373
BsiHKAI	GWGCWC	1 cut(s)	420
BslFI	GGGAC	1 cut(s)	227
BslI	CCNNNNNNNGG	2 cut(s)	354, 380
BsmFI	GGGAC	1 cut(s)	227
BsnI	GGCC	1 cut(s)	373
Bsp119I	TTCGAA	1 cut(s)	501
Bsp1286I	GDGCHC	1 cut(s)	420
Bsp143I	GATC	6 cut(s)	3, 195, 331, 352, 491, 619
BspACI	CCGC	1 cut(s)	348
BspANI	GGCC	1 cut(s)	373
BspCNI	CTCAG	2 cut(s)	201, 613
BspLI	GGNNCC	3 cut(s)	28, 215, 398
BspPI	GGATC	3 cut(s)	190, 339, 614
BspT104I	TTCGAA	1 cut(s)	501
BssECI	CCNNGG	2 cut(s)	73, 235
BssMI	GATC	6 cut(s)	3, 195, 331, 352, 491, 619
BssT1I	CCWWGG	1 cut(s)	73
Bst2UI	CCWGG	1 cut(s)	431
Bst4CI	ACNGT	3 cut(s)	97, 236, 498
Bst6I	CTCTTC	1 cut(s)	320
BstBAI	YACGTR	1 cut(s)	379
BstBI	TTCGAA	1 cut(s)	501
BstDEI	CTNAG	3 cut(s)	83, 209, 600
BstDSI	CCRYGG	1 cut(s)	235
BstF5I	GGATG	1 cut(s)	196
BstHHI	GCGC	1 cut(s)	157
BstKTI	GATC	6 cut(s)	6, 198, 334, 355, 494, 622
BstMBI	GATC	6 cut(s)	3, 195, 331, 352, 491, 619
BstMWI	GCNNNNNNNGC	1 cut(s)	635
BstNI	CCWGG	1 cut(s)	431
BstSCI	CCNGG	1 cut(s)	429
BstSNI	TACGTA	1 cut(s)	379
BstV1I	GCAGC	1 cut(s)	650
BstX2I	RGATCY	2 cut(s)	195, 331
BstXI	CCANNNNNNTGG	1 cut(s)	278
BstYI	RGATCY	2 cut(s)	195, 331
BsuRI	GGCC	1 cut(s)	373
BtgI	CCRYGG	1 cut(s)	235
BtsCI	GGATG	1 cut(s)	196
CfoI	GCGC	1 cut(s)	157
Cfr13I	GGNCC	2 cut(s)	214, 397
CspCI	CAANNNNNGTGG	2 cut(s)	206, 241
CviAII	CATG	5 cut(s)	40, 278, 292, 301, 521
DdeI	CTNAG	3 cut(s)	83, 209, 600
DpnI	GATC	6 cut(s)	5, 197, 333, 354, 493, 621
DpnII	GATC	6 cut(s)	3, 195, 331, 352, 491, 619
Eam1104I	CTCTTC	1 cut(s)	320
EarI	CTCTTC	1 cut(s)	320
Ecl136II	GAGCTC	1 cut(s)	418
Eco105I	TACGTA	1 cut(s)	379
Eco130I	CCWWGG	1 cut(s)	73
Eco24I	GRGCYC	1 cut(s)	420
Eco47I	GGWCC	2 cut(s)	214, 397
Eco53kI	GAGCTC	1 cut(s)	418
EcoICRI	GAGCTC	1 cut(s)	418
EcoRII	CCWGG	1 cut(s)	429
EcoT14I	CCWWGG	1 cut(s)	73
EcoT38I	GRGCYC	1 cut(s)	420
ErhI	CCWWGG	1 cut(s)	73
FaeI	CATG	5 cut(s)	43, 281, 295, 304, 524
FaqI	GGGAC	1 cut(s)	227
FatI	CATG	5 cut(s)	39, 277, 291, 300, 520
FbaI	TGATCA	1 cut(s)	3
Fnu4HI	GCNGC	1 cut(s)	639
FokI	GGATG	1 cut(s)	203
FriOI	GRGCYC	1 cut(s)	420
Fsp4HI	GCNGC	1 cut(s)	639
GlaI	GCGC	1 cut(s)	156
GluI	GCNGC	1 cut(s)	639
HaeIII	GGCC	1 cut(s)	373
HhaI	GCGC	1 cut(s)	157
Hin1II	CATG	5 cut(s)	43, 281, 295, 304, 524
Hin6I	GCGC	1 cut(s)	155
HinP1I	GCGC	1 cut(s)	155
HinfI	GANTC	5 cut(s)	344, 365, 391, 404, 604
HphI	GGTGA	2 cut(s)	486, 509
Hpy166II	GTNNAC	1 cut(s)	286
Hpy188I	TCNGA	5 cut(s)	146, 463, 484, 603, 614
Hpy188III	TCNNGA	1 cut(s)	553
Hpy8I	GTNNAC	1 cut(s)	286
HpyAV	CCTTC	1 cut(s)	538
HpyCH4III	ACNGT	3 cut(s)	97, 236, 498
HpyCH4IV	ACGT	2 cut(s)	288, 378
HpyCH4V	TGCA	1 cut(s)	310
HpyF10VI	GCNNNNNNNGC	1 cut(s)	635
HpyF3I	CTNAG	3 cut(s)	83, 209, 600
HpySE526I	ACGT	2 cut(s)	288, 378
Hsp92II	CATG	5 cut(s)	43, 281, 295, 304, 524
HspAI	GCGC	1 cut(s)	155
Ksp22I	TGATCA	1 cut(s)	3
Kzo9I	GATC	6 cut(s)	3, 195, 331, 352, 491, 619
LmnI	GCTCC	1 cut(s)	26
LpnPI	CCDG	7 cut(s)	49, 108, 186, 416, 443, 463, 538
Lsp1109I	GCAGC	1 cut(s)	650
LweI	GCATC	1 cut(s)	150
MaeII	ACGT	2 cut(s)	288, 378
MaeIII	GTNAC	2 cut(s)	148, 515
MalI	GATC	6 cut(s)	5, 197, 333, 354, 493, 621
MboI	GATC	6 cut(s)	3, 195, 331, 352, 491, 619
MboII	GAAGA	3 cut(s)	127, 252, 307
MflI	RGATCY	2 cut(s)	195, 331
MhlI	GDGCHC	1 cut(s)	420
MluCI	AATT	1 cut(s)	19
MlyI	GAGTC	3 cut(s)	374, 400, 413
MnlI	CCTC	8 cut(s)	99, 128, 204, 209, 363, 526, 565, 642
MroXI	GAANNNNTTC	2 cut(s)	168, 548
MseI	TTAA	1 cut(s)	102
MslI	CAYNNNNRTG	2 cut(s)	305, 439
MspA1I	CMGCKG	1 cut(s)	176
MspR9I	CCNGG	1 cut(s)	431
MvaI	CCWGG	1 cut(s)	431
MwoI	GCNNNNNNNGC	1 cut(s)	635
NdeII	GATC	6 cut(s)	3, 195, 331, 352, 491, 619
NlaIII	CATG	5 cut(s)	43, 281, 295, 304, 524
NlaIV	GGNNCC	3 cut(s)	28, 215, 398
NmuCI	GTSAC	1 cut(s)	515
NspV	TTCGAA	1 cut(s)	501
OliI	CACNNNNGTG	1 cut(s)	439
PdmI	GAANNNNTTC	2 cut(s)	168, 548
PfeI	GAWTC	2 cut(s)	344, 604
PkrI	GCNGC	1 cut(s)	640
PleI	GAGTC	3 cut(s)	373, 399, 412
PpsI	GAGTC	3 cut(s)	373, 399, 412
Ppu21I	YACGTR	1 cut(s)	379
Psp124BI	GAGCTC	1 cut(s)	420
Psp6I	CCWGG	1 cut(s)	429
PspGI	CCWGG	1 cut(s)	429
PspN4I	GGNNCC	3 cut(s)	28, 215, 398
PspPI	GGNCC	2 cut(s)	214, 397
PsuI	RGATCY	2 cut(s)	195, 331
PvuII	CAGCTG	1 cut(s)	176
RseI	CAYNNNNRTG	2 cut(s)	305, 439
SacI	GAGCTC	1 cut(s)	420
SaqAI	TTAA	1 cut(s)	102
SatI	GCNGC	1 cut(s)	639
Sau3AI	GATC	6 cut(s)	3, 195, 331, 352, 491, 619
Sau96I	GGNCC	2 cut(s)	214, 397
SchI	GAGTC	3 cut(s)	374, 400, 413
ScrFI	CCNGG	1 cut(s)	431
SduI	GDGCHC	1 cut(s)	420
SfaNI	GCATC	1 cut(s)	150
SfuI	TTCGAA	1 cut(s)	501
SinI	GGWCC	2 cut(s)	214, 397
SmiMI	CAYNNNNRTG	2 cut(s)	305, 439
SmlI	CTYRAG	1 cut(s)	419
SmoI	CTYRAG	1 cut(s)	419
SnaBI	TACGTA	1 cut(s)	379
Sse9I	AATT	1 cut(s)	19
SsiI	CCGC	1 cut(s)	348
SstI	GAGCTC	1 cut(s)	420
StyD4I	CCNGG	1 cut(s)	429
StyI	CCWWGG	1 cut(s)	73
TaaI	ACNGT	3 cut(s)	97, 236, 498
TaiI	ACGT	2 cut(s)	291, 381
TaqI	TCGA	4 cut(s)	368, 389, 414, 501
TasI	AATT	1 cut(s)	19
TfiI	GAWTC	2 cut(s)	344, 604
Tru1I	TTAA	1 cut(s)	102
Tru9I	TTAA	1 cut(s)	102
TseFI	GTSAC	1 cut(s)	515
TseI	GCWGC	1 cut(s)	638
Tsp45I	GTSAC	1 cut(s)	515
TspDTI	ATGAA	2 cut(s)	28, 648
TspGWI	ACGGA	1 cut(s)	389
VpaK11BI	GGWCC	2 cut(s)	214, 397
XcmI	CCANNNNNNNNNTGG	1 cut(s)	37
XmnI	GAANNNNTTC	2 cut(s)	168, 548

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.