RLG00000004559

Belongs to the malate synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0022326
Physical Location & Seq
Reverse (-)
60292459 .. 60293540
1082 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004559

Sequence Viewer

Length: 639 bp
ATGTGGAATTGTGTCTTTGAGAAGGCAGAGAAGATGGTAGGAATTGAGAGAGGAAGCATCAGAGCCACTGTTCTGATCAAAATACTTCCAGCTGTGTTTCAAATGGATGAAATCCTGTATGAGCTTAGGGATCACTCTGTTGGTTTGAACTGTGGAAGATGGGATTACATTTTCAGCTATGTCAAGACCTTCCAGGCTCACCCTGACCGCCTATTGCCTGACTGTGTTCTAGCTGGCATGAATCAACATTTCATGAGGACTTACTCTGGTCTCCTCATCAGGACCTCCCATAGGCGCGGTGACCACGCCATGGGAGGCATGGCAGCTCAGATTCCAATTAGAGATGATCCAGTAGCAAATGAAGCAGCATTGGATTTGGTGAGGAAGGACAAGCTAAGAGAAGTGAAAGCAGGACATGATGGAACTTGGGCAGCACATCCAGGACTAATCCCAGCCTGCATGGAAATCTTCACAAACAACATGGGCCACAACACCCCCAACCAAATCCAAACCATGAGGCGAGAAGATGCATCCTCCATAACCGAAGAAGACCTCTTGCAGAGGCCCAGAAGGGTCCGAACCCTGGAGGGTCTCCGGCTGAACACCTGGGTGGAATCCAGTACCCGGCAGCATGGCTAA

Protein Analysis

213

Amino Acids

24.18

Weight (kDa)

7.78

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_TIM-barrel PF01274 1 - 187 2.2e-79 Malate synthase, TIM barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022326)

Species Orthologous Gene IDs
malus_domestica MD14G1161700.v1.1
pyrus_communis pycom14g13470 pycom72g00080
rosa_laevigata RLG00000004559

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 310
AccII CGCG 1 cut(s) 297
AciI CCGC 2 cut(s) 208, 297
AclWI GGATC 2 cut(s) 138, 341
AfaI GTAC 1 cut(s) 622
AfiI CCNNNNNNNGG 4 cut(s) 291, 310, 583, 624
AgsI TTSAA 2 cut(s) 101, 148
AjnI CCWGG 4 cut(s) 192, 439, 582, 605
AleI CACNNNNGTG 1 cut(s) 608
AluBI AGCT 6 cut(s) 92, 124, 177, 233, 326, 394
AluI AGCT 6 cut(s) 92, 124, 177, 233, 326, 394
Alw26I GTCTC 2 cut(s) 275, 596
AlwI GGATC 2 cut(s) 138, 341
AoxI GGCC 2 cut(s) 484, 563
ApeKI GCWGC 4 cut(s) 323, 365, 431, 628
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 4 cut(s) 282, 484, 564, 574
AsuC2I CCSGG 1 cut(s) 625
AsuHPI GGTGA 3 cut(s) 191, 311, 391
AvaII GGWCC 2 cut(s) 282, 574
BbsI GAAGAC 1 cut(s) 555
BbvI GCAGC 3 cut(s) 335, 377, 443
BccI CCATC 3 cut(s) 28, 153, 413
BciT130I CCWGG 4 cut(s) 194, 441, 584, 607
BclI TGATCA 1 cut(s) 75
BcnI CCSGG 1 cut(s) 625
BcoDI GTCTC 2 cut(s) 275, 596
BfaI CTAG 1 cut(s) 230
BisI GCNGC 4 cut(s) 324, 366, 432, 629
BlsI GCNGC 4 cut(s) 325, 367, 433, 630
Bme1390I CCNGG 5 cut(s) 194, 441, 584, 607, 625
Bme18I GGWCC 2 cut(s) 282, 574
BmgT120I GGNCC 4 cut(s) 282, 484, 564, 574
BmiI GGNNCC 1 cut(s) 575
BmrFI CCNGG 5 cut(s) 194, 441, 584, 607, 625
BmsI GCATC 3 cut(s) 66, 517, 539
BpiI GAAGAC 1 cut(s) 555
BpmI CTGGAG 1 cut(s) 605
Bpu10I CCTNAGC 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 625
BsaI GGTCTC 2 cut(s) 275, 596
BsaJI CCNNGG 3 cut(s) 309, 582, 606
Bsc4I CCNNNNNNNGG 4 cut(s) 291, 310, 583, 624
Bse1I ACTGG 2 cut(s) 350, 618
BseBI CCWGG 4 cut(s) 194, 441, 584, 607
BseDI CCNNGG 3 cut(s) 309, 582, 606
BseGI GGATG 3 cut(s) 112, 436, 530
BseLI CCNNNNNNNGG 4 cut(s) 291, 310, 583, 624
BseMII CTCAG 1 cut(s) 341
BseNI ACTGG 2 cut(s) 350, 618
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 3 cut(s) 335, 377, 443
BseYI CCCAGC 1 cut(s) 451
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 2 cut(s) 486, 565
BsiSI CCGG 2 cut(s) 595, 625
BslI CCNNNNNNNGG 4 cut(s) 291, 310, 583, 624
BsmAI GTCTC 2 cut(s) 275, 596
BsnI GGCC 2 cut(s) 486, 565
Bso31I GGTCTC 2 cut(s) 275, 596
Bsp143I GATC 3 cut(s) 75, 130, 346
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 2 cut(s) 208, 297
BspANI GGCC 2 cut(s) 486, 565
BspCNI CTCAG 1 cut(s) 340
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 575
BspPI GGATC 2 cut(s) 138, 341
BspTNI GGTCTC 2 cut(s) 275, 596
BsrI ACTGG 2 cut(s) 350, 618
BssECI CCNNGG 3 cut(s) 309, 582, 606
BssMI GATC 3 cut(s) 75, 130, 346
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 4 cut(s) 194, 441, 584, 607
Bst4CI ACNGT 3 cut(s) 70, 152, 224
BstC8I GCNNGC 2 cut(s) 235, 457
BstDEI CTNAG 3 cut(s) 125, 327, 395
BstDSI CCRYGG 1 cut(s) 309
BstEII GGTNACC 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 289
BstF5I GGATG 3 cut(s) 112, 436, 530
BstFNI CGCG 1 cut(s) 297
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 3 cut(s) 78, 133, 349
BstMAI GTCTC 2 cut(s) 275, 596
BstMBI GATC 3 cut(s) 75, 130, 346
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 4 cut(s) 194, 441, 584, 607
BstPI GGTNACC 1 cut(s) 299
BstSCI CCNGG 5 cut(s) 192, 439, 582, 605, 623
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 3 cut(s) 335, 377, 443
BstV2I GAAGAC 1 cut(s) 555
BsuRI GGCC 2 cut(s) 486, 565
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 3 cut(s) 112, 436, 530
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 2 cut(s) 235, 457
CciI TCATGA 1 cut(s) 252
CfoI GCGC 1 cut(s) 297
Cfr13I GGNCC 4 cut(s) 282, 484, 564, 574
Csp6I GTAC 1 cut(s) 621
CviAII CATG 9 cut(s) 238, 253, 310, 319, 416, 460, 481, 514, 632
CviQI GTAC 1 cut(s) 621
DdeI CTNAG 3 cut(s) 125, 327, 395
DpnI GATC 3 cut(s) 77, 132, 348
DpnII GATC 3 cut(s) 75, 130, 346
Eco130I CCWWGG 1 cut(s) 309
Eco31I GGTCTC 2 cut(s) 275, 596
Eco47I GGWCC 2 cut(s) 282, 574
Eco91I GGTNACC 1 cut(s) 299
EcoNI CCTNNNNNAGG 1 cut(s) 289
EcoO109I RGGNCCY 1 cut(s) 282
EcoO65I GGTNACC 1 cut(s) 299
EcoRII CCWGG 4 cut(s) 192, 439, 582, 605
EcoT14I CCWWGG 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 9 cut(s) 241, 256, 313, 322, 419, 463, 484, 517, 635
FatI CATG 9 cut(s) 237, 252, 309, 318, 415, 459, 480, 513, 631
FbaI TGATCA 1 cut(s) 75
Fnu4HI GCNGC 4 cut(s) 324, 366, 432, 629
FokI GGATG 3 cut(s) 119, 423, 517
Fsp4HI GCNGC 4 cut(s) 324, 366, 432, 629
FspBI CTAG 1 cut(s) 230
GlaI GCGC 1 cut(s) 296
GluI GCNGC 4 cut(s) 324, 366, 432, 629
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 1 cut(s) 605
HaeIII GGCC 2 cut(s) 486, 565
HapII CCGG 2 cut(s) 595, 625
HhaI GCGC 1 cut(s) 297
Hin1II CATG 9 cut(s) 241, 256, 313, 322, 419, 463, 484, 517, 635
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HinfI GANTC 3 cut(s) 241, 331, 614
HpaII CCGG 2 cut(s) 595, 625
HphI GGTGA 3 cut(s) 191, 311, 391
Hpy188I TCNGA 4 cut(s) 62, 75, 330, 578
Hpy188III TCNNGA 3 cut(s) 184, 253, 280
HpyAV CCTTC 4 cut(s) 16, 199, 379, 564
HpyCH4III ACNGT 3 cut(s) 70, 152, 224
HpyCH4V TGCA 3 cut(s) 459, 530, 559
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 3 cut(s) 125, 327, 395
Hsp92II CATG 9 cut(s) 241, 256, 313, 322, 419, 463, 484, 517, 635
HspAI GCGC 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 75
Kzo9I GATC 3 cut(s) 75, 130, 346
Lsp1109I GCAGC 3 cut(s) 335, 377, 443
LweI GCATC 3 cut(s) 66, 517, 539
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 1 cut(s) 299
MalI GATC 3 cut(s) 77, 132, 348
MboI GATC 3 cut(s) 75, 130, 346
MboII GAAGA 6 cut(s) 43, 168, 460, 536, 557, 560
MluCI AATT 3 cut(s) 7, 42, 336
Mph1103I ATGCAT 1 cut(s) 532
MslI CAYNNNNRTG 1 cut(s) 608
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 2 cut(s) 595, 625
MspR9I CCNGG 5 cut(s) 194, 441, 584, 607, 625
MvaI CCWGG 4 cut(s) 194, 441, 584, 607
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 362
NciI CCSGG 1 cut(s) 625
NcoI CCATGG 1 cut(s) 309
NdeII GATC 3 cut(s) 75, 130, 346
NlaIII CATG 9 cut(s) 241, 256, 313, 322, 419, 463, 484, 517, 635
NlaIV GGNNCC 1 cut(s) 575
NmuCI GTSAC 1 cut(s) 299
NsiI ATGCAT 1 cut(s) 532
OliI CACNNNNGTG 1 cut(s) 608
PagI TCATGA 1 cut(s) 252
PfeI GAWTC 3 cut(s) 241, 331, 614
PflMI CCANNNNNTGG 1 cut(s) 310
PfoI TCCNGGA 1 cut(s) 439
PkrI GCNGC 4 cut(s) 325, 367, 433, 630
PpuMI RGGWCCY 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 282
Psp6I CCWGG 4 cut(s) 192, 439, 582, 605
PspEI GGTNACC 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 4 cut(s) 192, 439, 582, 605
PspN4I GGNNCC 1 cut(s) 575
PspPI GGNCC 4 cut(s) 282, 484, 564, 574
PspPPI RGGWCCY 1 cut(s) 282
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 1 cut(s) 622
RsaNI GTAC 1 cut(s) 621
RseI CAYNNNNRTG 1 cut(s) 608
SatI GCNGC 4 cut(s) 324, 366, 432, 629
Sau3AI GATC 3 cut(s) 75, 130, 346
Sau96I GGNCC 4 cut(s) 282, 484, 564, 574
ScrFI CCNGG 5 cut(s) 194, 441, 584, 607, 625
SfaNI GCATC 3 cut(s) 66, 517, 539
SinI GGWCC 2 cut(s) 282, 574
SmiMI CAYNNNNRTG 1 cut(s) 608
Sse9I AATT 3 cut(s) 7, 42, 336
SsiI CCGC 2 cut(s) 208, 297
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 5 cut(s) 192, 439, 582, 605, 623
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 3 cut(s) 70, 152, 224
TasI AATT 3 cut(s) 7, 42, 336
TfiI GAWTC 3 cut(s) 241, 331, 614
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 4 cut(s) 323, 365, 431, 628
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 4 cut(s) 123, 241, 254, 375
TspRI CASTG 1 cut(s) 73
Van91I CCANNNNNTGG 1 cut(s) 310
VpaK11BI GGWCC 2 cut(s) 282, 574
XagI CCTNNNNNAGG 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 316
XspI CTAG 1 cut(s) 230
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.