MD14G1196900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0024273
Physical Location & Seq
Reverse (-)
28699905 .. 28701614
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1196900.v1.1.491

Sequence Viewer

Length: 1710 bp
ATGTCTCCCAAACCCTCCATCATCAAATCAAACTGCAACCCTTTTGATGAAATAACATCACTCTTGCAAAAATGCAAACACAAAGACCATCTCCATCAAATCCATGCACGCATCGTCACCACAGGCCTCGTCCGCAAGCCTTCTGTAGCAAGCAAACTTGTTGCATCTCTTGCCTCCATTCCCCTCCCCGCCACAAACTCCGCCGCCCGCTCGATCGCCGACGGAGTCAAGGGTCTCGATTCCTACACCTGGAACACCATCATCAGAGGTCACTTGGCGGGGAATCAACCAAAAGAAGCGCTTTTGGTTTATTCCCATGTCAGAGGGAAGGGTCTGGAGGTGGATAGTTATACCCTTCAGTTTGCTATCAAGGCCTGCCGCCTGATGCCATTGGTTCTTGAAGGGAAGCAGTTACATAACCAGATTTTGAAACTGGGTTTTGGCTCCGAGATCATAATTCAAACTTCCCTTGTGAATTTGTATGGTTTTTTCGGTGAGCTTGATTGTATGCAGCAAGTGTTTGATGAAACGCCTCAGAGAGATTTAGTCATGTGGAATTCGGTTGTTGCTGCATATTCTCAGCACAATTTCCCTTACATGGCACTTGCAGTTGCCAGTGCCATGGCCCGTGAAGGTTTGAGACTAAATGGGTCGAGTGTCGTTAGCTTGCTTTCCGCTTGCTCGTTTTTGAAGGCGATAAGGAAAGGGAAGGAAGTTCATGGCTATGTGATTAGAGGACAGCTTGTCGATAATCATGATGTTTTTGTTTACAATGCTTTGATTAGTATGTATTCAAGATGCGGGGTTTTATCAAGTGCTCGTCGGATATTTCAAACGATGCCTATGAAAAATGTGGTTTCTTGGACTTCTATGATCAACTGTTATAGCGATAACGATCATCTGAAGGAGGCATTCTGGTTGTTTGAACAGATGGAGTCTGAGAAAATAAAACCAGATGAGATTACCATTTTGGGAGTTATTTCAATGTCCTCGAAGTTTCGAAGCTTCAAGATCGCCGAGTGGATTGACCGTTATGTCGAGAGAAACGGGTTTAGAAGTACAGGGAGCATTGCCATGTTAAATGCACTGATGGACATGCATGCCAAATGCGGGAACATCAAGAAAGCTTGTGAAATTTTTTATGGCATGGAACAGAAATCGTTAATGTCTTGGACTACCATCATACACGGACTAGCGATGCACAGCGATGGAAAGCAAGCGCTGGCTATGTTCTCTCAGATGCAAAGAGAAGGGTGTAAACCAGATGGGGTTGTATTCCTCAACATATTGTCTGCCTGCAGCCACGCAGGAATGGTGGACGAGGCGCGGAATTGTTTCAATTCAATGGTAAACGATTGCCACATGAAACCGCGGATGGAGCACTACGGTTGCATGGTTGATCTACTATGCAGAGCTGGATTGGTGAGTGAGGCATTTGAGTTTGTTCAGACCATGCCGGATAAACCAGATACGATGATATGGCGAATGCTGCTCGGGGCATGTCAAGGCCAAGGAGATGGTAGTTTAGCAAGACGGATTTTGAGCCAGTTACGTGAGATAGGGCCGAAAAACAGCAGAGACTACGGACTGCTATCAAATTTGTATGCCGCCATGGATGAATGGGATAATGTGAAGGAGATAAGAAGGGAGATGAAGGAGAAGGGAGTAATAAAAGAGGATCCTGGGAGCAGTTCAATTGAGGTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

570

Amino Acids

63.72

Weight (kDa)

8.56

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 80 - 126 1.1e-06 PPR repeat family
PPR_2 PF13041 283 - 323 1.1e-10 PPR repeat family
PPR PF01535 286 - 315 1.9e-07 PPR repeat
PPR_2 PF13041 389 - 434 5e-08 PPR repeat family
E_motif PF20431 509 - 567 4.7e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024273)

Species Orthologous Gene IDs
malus_domestica MD14G1196900.v1.1
prunus_persica Prupe.5G189100_v2.0.a1
pyrus_communis pycom14g16360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1034
AccBSI CCGCTC 1 cut(s) 210
AccII CGCG 2 cut(s) 1327, 1372
AclWI GGATC 2 cut(s) 1673, 1686
AcsI RAATTY 4 cut(s) 475, 556, 1134, 1597
AcuI CTGAAG 2 cut(s) 341, 923
AfaI GTAC 1 cut(s) 1060
AfeI AGCGCT 2 cut(s) 300, 1221
AfiI CCNNNNNNNGG 3 cut(s) 249, 598, 1110
AhdI GACNNNNNGTC 1 cut(s) 743
AjnI CCWGG 2 cut(s) 248, 1681
AluBI AGCT 6 cut(s) 499, 666, 742, 1005, 1127, 1415
AluI AGCT 6 cut(s) 499, 666, 742, 1005, 1127, 1415
Alw21I GWGCWC 2 cut(s) 820, 1383
Alw26I GTCTC 4 cut(s) 9, 239, 634, 1572
AlwI GGATC 2 cut(s) 1673, 1686
Ama87I CYCGRG 1 cut(s) 1493
Aor51HI AGCGCT 2 cut(s) 300, 1221
AoxI GGCC 5 cut(s) 124, 372, 624, 1507, 1562
ApeKI GCWGC 4 cut(s) 511, 569, 1299, 1489
ApoI RAATTY 4 cut(s) 475, 556, 1134, 1597
Asp700I GAANNNNTTC 1 cut(s) 1334
AspLEI GCGC 3 cut(s) 301, 1222, 1327
AspS9I GGNCC 2 cut(s) 625, 1562
AsuHPI GGTGA 3 cut(s) 109, 506, 1435
AsuII TTCGAA 1 cut(s) 1000
AvaI CYCGRG 1 cut(s) 1493
BamHI GGATCC 1 cut(s) 1678
Bbv12I GWGCWC 2 cut(s) 820, 1383
BbvI GCAGC 4 cut(s) 523, 556, 1311, 1476
BciT130I CCWGG 2 cut(s) 250, 1683
BclI TGATCA 1 cut(s) 873
BcoDI GTCTC 4 cut(s) 9, 239, 634, 1572
BfaI CTAG 1 cut(s) 1193
BfmI CTRYAG 2 cut(s) 144, 1297
BfoI RGCGCY 2 cut(s) 302, 1223
BisI GCNGC 7 cut(s) 204, 379, 512, 570, 1300, 1490, 1608
BlsI GCNGC 7 cut(s) 205, 380, 513, 571, 1301, 1491, 1609
Bme1390I CCNGG 2 cut(s) 250, 1683
BmeRI GACNNNNNGTC 1 cut(s) 743
BmeT110I CYCGRG 1 cut(s) 1493
BmgT120I GGNCC 2 cut(s) 625, 1562
BmiI GGNNCC 2 cut(s) 445, 1680
BmrFI CCNGG 2 cut(s) 250, 1683
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 7 cut(s) 120, 173, 375, 788, 828, 1188, 1230
BmuI ACTGGG 1 cut(s) 443
BpmI CTGGAG 1 cut(s) 356
Bpu14I TTCGAA 1 cut(s) 1000
BsaAI YACGTR 1 cut(s) 1553
BsaBI GATNNNNATC 1 cut(s) 894
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 5 cut(s) 621, 1370, 1510, 1611, 1682
Bsc4I CCNNNNNNNGG 3 cut(s) 249, 598, 1110
Bse1I ACTGG 3 cut(s) 438, 615, 1546
Bse3DI GCAATG 1 cut(s) 1068
Bse8I GATNNNNATC 1 cut(s) 894
BseBI CCWGG 2 cut(s) 250, 1683
BseDI CCNNGG 5 cut(s) 621, 1370, 1510, 1611, 1682
BseGI GGATG 2 cut(s) 1380, 1621
BseJI GATNNNNATC 1 cut(s) 894
BseLI CCNNNNNNNGG 3 cut(s) 249, 598, 1110
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 4 cut(s) 548, 593, 930, 1250
BseNI ACTGG 3 cut(s) 438, 615, 1546
BseXI GCAGC 4 cut(s) 523, 556, 1311, 1476
Bsh1236I CGCG 2 cut(s) 1327, 1372
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 5 cut(s) 126, 374, 626, 1509, 1564
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 2 cut(s) 820, 1383
BsiHKCI CYCGRG 1 cut(s) 1493
BsiSI CCGG 1 cut(s) 1457
BslI CCNNNNNNNGG 3 cut(s) 249, 598, 1110
BsmAI GTCTC 4 cut(s) 9, 239, 634, 1572
BsmI GAATGC 2 cut(s) 911, 1491
BsnI GGCC 5 cut(s) 126, 374, 626, 1509, 1564
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 1493
Bsp119I TTCGAA 1 cut(s) 1000
Bsp1286I GDGCHC 2 cut(s) 820, 1383
Bsp143I GATC 7 cut(s) 213, 450, 873, 895, 1011, 1399, 1678
Bsp19I CCATGG 2 cut(s) 621, 1611
BspANI GGCC 5 cut(s) 126, 374, 626, 1509, 1564
BspCNI CTCAG 4 cut(s) 547, 592, 931, 1249
BspFNI CGCG 2 cut(s) 1327, 1372
BspHI TCATGA 1 cut(s) 754
BspLI GGNNCC 2 cut(s) 445, 1680
BspMAI CTGCAG 1 cut(s) 1301
BspPI GGATC 2 cut(s) 1673, 1686
BspT104I TTCGAA 1 cut(s) 1000
BspTNI GGTCTC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 210
BsrDI GCAATG 1 cut(s) 1068
BsrI ACTGG 3 cut(s) 438, 615, 1546
BssECI CCNNGG 5 cut(s) 621, 1370, 1510, 1611, 1682
BssMI GATC 7 cut(s) 213, 450, 873, 895, 1011, 1399, 1678
BssT1I CCWWGG 3 cut(s) 621, 1510, 1611
Bst2UI CCWGG 2 cut(s) 250, 1683
Bst4CI ACNGT 3 cut(s) 881, 1031, 1388
BstAPI GCANNNNNTGC 1 cut(s) 170
BstBAI YACGTR 1 cut(s) 1553
BstBI TTCGAA 1 cut(s) 1000
BstDEI CTNAG 4 cut(s) 534, 579, 939, 1236
BstDSI CCRYGG 3 cut(s) 621, 1370, 1611
BstF5I GGATG 2 cut(s) 1380, 1621
BstFNI CGCG 2 cut(s) 1327, 1372
BstH2I RGCGCY 2 cut(s) 302, 1223
BstHHI GCGC 3 cut(s) 301, 1222, 1327
BstKTI GATC 7 cut(s) 216, 453, 876, 898, 1014, 1402, 1681
BstMAI GTCTC 4 cut(s) 9, 239, 634, 1572
BstMBI GATC 7 cut(s) 213, 450, 873, 895, 1011, 1399, 1678
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 5 cut(s) 132, 170, 371, 1378, 1489
BstNI CCWGG 2 cut(s) 250, 1683
BstNSI RCATGY 3 cut(s) 1099, 1103, 1503
BstSCI CCNGG 2 cut(s) 248, 1681
BstSFI CTRYAG 2 cut(s) 144, 1297
BstUI CGCG 2 cut(s) 1327, 1372
BstV1I GCAGC 4 cut(s) 523, 556, 1311, 1476
BstX2I RGATCY 1 cut(s) 1678
BstXI CCANNNNNNTGG 2 cut(s) 622, 1517
BstYI RGATCY 1 cut(s) 1678
BsuRI GGCC 5 cut(s) 126, 374, 626, 1509, 1564
BtgI CCRYGG 3 cut(s) 621, 1370, 1611
BtgZI GCGATG 2 cut(s) 1211, 1221
BtsCI GGATG 2 cut(s) 1380, 1621
BtsIMutI CAGTG 2 cut(s) 622, 1085
CciI TCATGA 1 cut(s) 754
CfoI GCGC 3 cut(s) 301, 1222, 1327
Cfr13I GGNCC 2 cut(s) 625, 1562
Cfr42I CCGCGG 1 cut(s) 1373
Csp6I GTAC 1 cut(s) 1059
CviQI GTAC 1 cut(s) 1059
DdeI CTNAG 4 cut(s) 534, 579, 939, 1236
DpnI GATC 7 cut(s) 215, 452, 875, 897, 1013, 1401, 1680
DpnII GATC 7 cut(s) 213, 450, 873, 895, 1011, 1399, 1678
DrdI GACNNNNNNGTC 1 cut(s) 1034
DriI GACNNNNNGTC 1 cut(s) 743
DseDI GACNNNNNNGTC 1 cut(s) 1034
Eam1105I GACNNNNNGTC 1 cut(s) 743
EciI GGCGGA 1 cut(s) 190
Eco130I CCWWGG 3 cut(s) 621, 1510, 1611
Eco147I AGGCCT 2 cut(s) 126, 374
Eco31I GGTCTC 1 cut(s) 239
Eco47III AGCGCT 2 cut(s) 300, 1221
Eco57I CTGAAG 2 cut(s) 341, 923
Eco88I CYCGRG 1 cut(s) 1493
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 2 cut(s) 248, 1681
EcoT14I CCWWGG 3 cut(s) 621, 1510, 1611
EcoT22I ATGCAT 1 cut(s) 1101
ErhI CCWWGG 3 cut(s) 621, 1510, 1611
FalI AAGNNNNNCTT 2 cut(s) 285, 317
FauI CCCGC 5 cut(s) 196, 215, 271, 794, 1103
FbaI TGATCA 1 cut(s) 873
Fnu4HI GCNGC 7 cut(s) 204, 379, 512, 570, 1300, 1490, 1608
FokI GGATG 2 cut(s) 1387, 1628
Fsp4HI GCNGC 7 cut(s) 204, 379, 512, 570, 1300, 1490, 1608
FspBI CTAG 1 cut(s) 1193
GlaI GCGC 3 cut(s) 300, 1221, 1326
GluI GCNGC 7 cut(s) 204, 379, 512, 570, 1300, 1490, 1608
GsuI CTGGAG 1 cut(s) 356
HaeII RGCGCY 2 cut(s) 302, 1223
HaeIII GGCC 5 cut(s) 126, 374, 626, 1509, 1564
HapII CCGG 1 cut(s) 1457
HhaI GCGC 3 cut(s) 301, 1222, 1327
Hin6I GCGC 3 cut(s) 299, 1220, 1325
HinP1I GCGC 3 cut(s) 299, 1220, 1325
HindIII AAGCTT 2 cut(s) 1003, 1125
HinfI GANTC 4 cut(s) 225, 239, 283, 935
HpaII CCGG 1 cut(s) 1457
HphI GGTGA 3 cut(s) 109, 506, 1435
Hpy166II GTNNAC 4 cut(s) 769, 1259, 1318, 1351
Hpy188I TCNGA 9 cut(s) 266, 323, 448, 537, 825, 903, 940, 1239, 1449
Hpy188III TCNNGA 8 cut(s) 236, 335, 398, 755, 795, 1009, 1039, 1120
Hpy8I GTNNAC 4 cut(s) 769, 1259, 1318, 1351
Hpy99I CGWCG 2 cut(s) 224, 825
HpyCH4III ACNGT 3 cut(s) 881, 1031, 1388
HpyCH4IV ACGT 1 cut(s) 1552
HpyF10VI GCNNNNNNNGC 5 cut(s) 132, 170, 371, 1378, 1489
HpyF3I CTNAG 4 cut(s) 534, 579, 939, 1236
HpySE526I ACGT 1 cut(s) 1552
HspAI GCGC 3 cut(s) 299, 1220, 1325
Ksp22I TGATCA 1 cut(s) 873
KspI CCGCGG 1 cut(s) 1373
Kzo9I GATC 7 cut(s) 213, 450, 873, 895, 1011, 1399, 1678
LmnI GCTCC 4 cut(s) 449, 1065, 1378, 1686
Lsp1109I GCAGC 4 cut(s) 523, 556, 1311, 1476
LweI GCATC 7 cut(s) 120, 173, 375, 788, 828, 1188, 1230
MaeI CTAG 1 cut(s) 1193
MaeII ACGT 1 cut(s) 1552
MaeIII GTNAC 4 cut(s) 115, 269, 411, 1548
MalI GATC 7 cut(s) 215, 452, 875, 897, 1013, 1401, 1680
MbiI CCGCTC 1 cut(s) 210
MboI GATC 7 cut(s) 213, 450, 873, 895, 1011, 1399, 1678
MfeI CAATTG 1 cut(s) 1695
MflI RGATCY 1 cut(s) 1678
MhlI GDGCHC 2 cut(s) 820, 1383
MluCI AATT 9 cut(s) 456, 475, 556, 586, 1134, 1330, 1339, 1597, 1695
MlyI GAGTC 2 cut(s) 234, 944
MmeI TCCRAC 1 cut(s) 803
Mph1103I ATGCAT 1 cut(s) 1101
MroXI GAANNNNTTC 1 cut(s) 1334
MseI TTAA 3 cut(s) 1079, 1163, 1708
MslI CAYNNNNRTG 3 cut(s) 723, 1073, 1206
MspA1I CMGCKG 1 cut(s) 1372
MspI CCGG 1 cut(s) 1457
MspR9I CCNGG 2 cut(s) 250, 1683
MunI CAATTG 1 cut(s) 1695
Mva1269I GAATGC 2 cut(s) 911, 1491
MvaI CCWGG 2 cut(s) 250, 1683
MvnI CGCG 2 cut(s) 1327, 1372
MwoI GCNNNNNNNGC 5 cut(s) 132, 170, 371, 1378, 1489
NcoI CCATGG 2 cut(s) 621, 1611
NdeII GATC 7 cut(s) 213, 450, 873, 895, 1011, 1399, 1678
NlaIV GGNNCC 2 cut(s) 445, 1680
NmeAIII GCCGAG 1 cut(s) 1042
NmuCI GTSAC 2 cut(s) 115, 269
NsiI ATGCAT 1 cut(s) 1101
NspI RCATGY 3 cut(s) 1099, 1103, 1503
NspV TTCGAA 1 cut(s) 1000
PaeI GCATGC 1 cut(s) 1103
PagI TCATGA 1 cut(s) 754
PceI AGGCCT 2 cut(s) 126, 374
PctI GAATGC 2 cut(s) 911, 1491
PdmI GAANNNNTTC 1 cut(s) 1334
PfeI GAWTC 2 cut(s) 239, 283
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 7 cut(s) 205, 380, 513, 571, 1301, 1491, 1609
Ple19I CGATCG 1 cut(s) 216
PleI GAGTC 2 cut(s) 233, 943
PpsI GAGTC 2 cut(s) 233, 943
Ppu21I YACGTR 1 cut(s) 1553
Psp6I CCWGG 2 cut(s) 248, 1681
PspGI CCWGG 2 cut(s) 248, 1681
PspN4I GGNNCC 2 cut(s) 445, 1680
PspPI GGNCC 2 cut(s) 625, 1562
PstI CTGCAG 1 cut(s) 1301
PsuI RGATCY 1 cut(s) 1678
PsyI GACNNNGTC 1 cut(s) 224
PvuI CGATCG 1 cut(s) 216
RsaI GTAC 1 cut(s) 1060
RsaNI GTAC 1 cut(s) 1059
RseI CAYNNNNRTG 3 cut(s) 723, 1073, 1206
SacII CCGCGG 1 cut(s) 1373
SaqAI TTAA 3 cut(s) 1079, 1163, 1708
SatI GCNGC 7 cut(s) 204, 379, 512, 570, 1300, 1490, 1608
Sau3AI GATC 7 cut(s) 213, 450, 873, 895, 1011, 1399, 1678
Sau96I GGNCC 2 cut(s) 625, 1562
SchI GAGTC 2 cut(s) 234, 944
ScrFI CCNGG 2 cut(s) 250, 1683
SduI GDGCHC 2 cut(s) 820, 1383
SfaNI GCATC 7 cut(s) 120, 173, 375, 788, 828, 1188, 1230
SfcI CTRYAG 2 cut(s) 144, 1297
Sfr303I CCGCGG 1 cut(s) 1373
SfuI TTCGAA 1 cut(s) 1000
SgrBI CCGCGG 1 cut(s) 1373
SmiMI CAYNNNNRTG 3 cut(s) 723, 1073, 1206
SphI GCATGC 1 cut(s) 1103
Sse9I AATT 9 cut(s) 456, 475, 556, 586, 1134, 1330, 1339, 1597, 1695
SseBI AGGCCT 2 cut(s) 126, 374
SspMI CTAG 1 cut(s) 1193
StuI AGGCCT 2 cut(s) 126, 374
StyD4I CCNGG 2 cut(s) 248, 1681
StyI CCWWGG 3 cut(s) 621, 1510, 1611
TaaI ACNGT 3 cut(s) 881, 1031, 1388
TaiI ACGT 1 cut(s) 1555
TaqI TCGA 7 cut(s) 212, 237, 653, 747, 992, 1000, 1038
TasI AATT 9 cut(s) 456, 475, 556, 586, 1134, 1330, 1339, 1597, 1695
TatI WGTACW 1 cut(s) 1058
TauI GCSGC 3 cut(s) 206, 381, 1610
TfiI GAWTC 2 cut(s) 239, 283
Tru1I TTAA 3 cut(s) 1079, 1163, 1708
Tru9I TTAA 3 cut(s) 1079, 1163, 1708
TscAI CASTG 2 cut(s) 622, 1092
TseFI GTSAC 2 cut(s) 115, 269
TseI GCWGC 4 cut(s) 511, 569, 1299, 1489
Tsp45I GTSAC 2 cut(s) 115, 269
TspDTI ATGAA 7 cut(s) 63, 540, 707, 860, 1379, 1632, 1667
TspGWI ACGGA 4 cut(s) 237, 1203, 1549, 1599
TspRI CASTG 2 cut(s) 622, 1092
Tth111I GACNNNGTC 1 cut(s) 224
XapI RAATTY 4 cut(s) 475, 556, 1134, 1597
XceI RCATGY 3 cut(s) 1099, 1103, 1503
XmnI GAANNNNTTC 1 cut(s) 1334
XspI CTAG 1 cut(s) 1193
Zsp2I ATGCAT 1 cut(s) 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.