Prupe.5G189100_v2.0.a1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0024273
Physical Location & Seq
Reverse (-)
15537857 .. 15539434
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G189100.1

Sequence Viewer

Length: 1578 bp
CTCGTTCACAACCTTCCAATAGCAAGCAAACTTGTTTCATCTTTTGCCTTATTTGCCCTCCCCGCCACAAATTTCACTGCCCATTTGATCGCCGACCGAGTAGACGGTCTCGATACCTACACCTGGAACACCATTATCAGAGGTTATTTGGCCGGAAATCGTCCAAAAGAAGCGCTTTTGGTTTATTCCCATGTGAGAAGAAAGGGTCTGGAGGTGGATAGTTATACCCTGCAGTTTGCTATTAAGGCATGCCGGCTTATGCCAATAGTTCTTGAAGGGAAACAGTTACATAACCACATTTTGAAACTGGGTTTTGTCTCTGAGATCATAATTCAAACTGCCCTTCTGAATATGTATGGTGTTTTTGGTGAGCTTAGATATATGCAACAAGTATTTGATGAAACGCCTCAGAGGGACTTAATTATGTGGAATTCTATTGTTGCAGCATATGCTCGGCACAATTTCCCTTATAAGGCACTTGCAGTAGCCAGTGCCATGGCCAGTGACGGTTTGAGACTAAATGGATTAAGTGTTGTTAGCTTGCTTTCTGCTTGCTCATCTTTGCAGGCCTTGAGGCAAGGGAAGGCAGTTCATGGCTATGCGATTAGAGGACACCTTCTTGATAATCATGATGTTTTTGTTTACAATGCTTTGGTTAGCATGTATTCAAAATGTGTGTTTTTATCAAATGCTAGTCGGGTATTCCAGATGATGCCTATAAGAAATGTGGTTTCTTGGACTTCTATGATCAATGGTTTTAGTGAGAACAATTATCTGAAGGAGGCATTGGCTCTGTTTGAAGAGATGGTGGCTAAGAATATAAGACCGGATGAGGTTACCATTTTTGGTGTTATTTCCATGTCCTCCAAGTTGCAAAGCTTCAAGCTCGGGGAGTGGATTGACCATTATATTGAGAGGAATGGATTTAGAACAGGGAGTATTGCCATGTCAAATGCACTGATGGACATGCATGCCAAACGTGGGAACATCAAGAAAGCTTGTCAAATTTTTGATGGGATGCCGGAGAAGTCGTTAGTGTCTTGGACTACTATAATACAAGGGCTAGCGATGCACGGCCTTGGAAGGCTAGCTCTGGCTCAGTTCTCTCAGATGCAAAACGAGGGCTTTAAGCCAGATGGGATTGTCTTCCTCAACATATTATCTGCCTGCAGCCACGCCGGTATGGTGGACGAGGGGAGAAAGTGTTTTAATTCAATGATAAATGATTTTCACATGAAACCATGGATGGAGCACTACGGTTGCATGGTTGATCTCCTATGCAGAGCTGGATTGGTAAGCGAGGCATTTGAGTTTGTTCAAAACATGCCAGATAAACCAGATACAATAATATGGCGCATGCTGCTTGGGGCATGTCAAGCCCAAGGAGAGGCTAGTCTAGCGAGTCAGATTATGAACCATTTACATGACATAGGGCCTAAAAATAGCGGAGACTATGGACTGCTGTCGAATTTGTATGCCGCCGTGGCCGAATGGGATAATGTCAAAGAGATAAGAAAGGAGATGAAAGAGAAGGGAGTCGTAAAACAAGATCCTGGTAGCAGTTCAATCGAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

526

Amino Acids

58.65

Weight (kDa)

8.05

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024273)

Species Orthologous Gene IDs
malus_domestica MD14G1196900.v1.1
prunus_persica Prupe.5G189100_v2.0.a1
pyrus_communis pycom14g16360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 471
AccI GTMKAC 1 cut(s) 102
AciI CCGC 3 cut(s) 63, 1446, 1479
AclWI GGATC 1 cut(s) 1544
AcoI YGGCCR 3 cut(s) 150, 498, 1485
AcsI RAATTY 4 cut(s) 70, 430, 1005, 1468
AcuI CTGAAG 1 cut(s) 797
AfeI AGCGCT 1 cut(s) 174
AfiI CCNNNNNNNGG 4 cut(s) 123, 472, 981, 1387
AgsI TTSAA 9 cut(s) 275, 304, 335, 669, 800, 883, 1215, 1319, 1566
AjnI CCWGG 2 cut(s) 122, 1552
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AluBI AGCT 7 cut(s) 373, 540, 879, 886, 998, 1091, 1286
AluI AGCT 7 cut(s) 373, 540, 879, 886, 998, 1091, 1286
Alw21I GWGCWC 1 cut(s) 1254
Alw26I GTCTC 4 cut(s) 113, 322, 508, 1443
AlwI GGATC 1 cut(s) 1544
Ama87I CYCGRG 1 cut(s) 887
Aor51HI AGCGCT 1 cut(s) 174
AoxI GGCC 6 cut(s) 150, 498, 567, 1075, 1433, 1485
ApeKI GCWGC 3 cut(s) 443, 1170, 1360
ApoI RAATTY 4 cut(s) 70, 430, 1005, 1468
AspLEI GCGC 2 cut(s) 175, 1356
AspS9I GGNCC 1 cut(s) 1433
AsuHPI GGTGA 1 cut(s) 380
AsuNHI GCTAGC 2 cut(s) 1063, 1087
AvaI CYCGRG 1 cut(s) 887
BalI TGGCCA 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 1138
Bbv12I GWGCWC 1 cut(s) 1254
BbvI GCAGC 3 cut(s) 455, 1182, 1347
BccI CCATC 5 cut(s) 799, 955, 1007, 1130, 1240
BceAI ACGGC 2 cut(s) 1090, 1466
BcgI CGANNNNNNTGC 1 cut(s) 1549
BciT130I CCWGG 2 cut(s) 124, 1554
BclI TGATCA 1 cut(s) 747
BcoDI GTCTC 4 cut(s) 113, 322, 508, 1443
BfaI CTAG 5 cut(s) 693, 1064, 1088, 1392, 1397
BfmI CTRYAG 2 cut(s) 230, 1168
BfoI RGCGCY 1 cut(s) 176
BglI GCCNNNNNGGC 1 cut(s) 1484
BisI GCNGC 4 cut(s) 444, 1171, 1361, 1479
BlsI GCNGC 4 cut(s) 445, 1172, 1362, 1480
Bme1390I CCNGG 2 cut(s) 124, 1554
BmeT110I CYCGRG 1 cut(s) 887
BmgT120I GGNCC 1 cut(s) 1433
BmrFI CCNGG 2 cut(s) 124, 1554
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 4 cut(s) 702, 1008, 1059, 1101
BmtI GCTAGC 2 cut(s) 1067, 1091
BmuI ACTGGG 1 cut(s) 317
BoxI GACNNNNGTC 1 cut(s) 1462
BpiI GAAGAC 1 cut(s) 1138
BpmI CTGGAG 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 592
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 5 cut(s) 495, 1078, 1241, 1381, 1482
BsaWI WCCGGW 1 cut(s) 826
BsaXI ACNNNNNCTCC 4 cut(s) 1016, 1046, 1189, 1219
Bsc4I CCNNNNNNNGG 4 cut(s) 123, 472, 981, 1387
Bse118I RCCGGY 2 cut(s) 252, 1178
Bse1I ACTGG 3 cut(s) 312, 489, 501
BseBI CCWGG 2 cut(s) 124, 1554
BseDI CCNNGG 5 cut(s) 495, 1078, 1241, 1381, 1482
BseGI GGATG 3 cut(s) 835, 1023, 1251
BseLI CCNNNNNNNGG 4 cut(s) 123, 472, 981, 1387
BseMII CTCAG 4 cut(s) 312, 422, 1112, 1121
BseNI ACTGG 3 cut(s) 312, 489, 501
BseXI GCAGC 3 cut(s) 455, 1182, 1347
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 6 cut(s) 152, 500, 569, 1077, 1435, 1487
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 1254
BsiHKCI CYCGRG 1 cut(s) 887
BsiSI CCGG 5 cut(s) 153, 253, 827, 1022, 1179
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 4 cut(s) 123, 472, 981, 1387
BsmAI GTCTC 4 cut(s) 113, 322, 508, 1443
BsmFI GGGAC 1 cut(s) 428
BsnI GGCC 6 cut(s) 152, 500, 569, 1077, 1435, 1487
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 887
Bsp1286I GDGCHC 1 cut(s) 1254
Bsp143I GATC 5 cut(s) 87, 324, 747, 1270, 1549
Bsp19I CCATGG 2 cut(s) 495, 1241
BspACI CCGC 3 cut(s) 63, 1446, 1479
BspANI GGCC 6 cut(s) 152, 500, 569, 1077, 1435, 1487
BspCNI CTCAG 4 cut(s) 313, 421, 1111, 1120
BspHI TCATGA 1 cut(s) 628
BspMAI CTGCAG 2 cut(s) 234, 1172
BspOI GCTAGC 2 cut(s) 1067, 1091
BspPI GGATC 1 cut(s) 1544
BspTNI GGTCTC 1 cut(s) 113
BsrFI RCCGGY 2 cut(s) 252, 1178
BsrI ACTGG 3 cut(s) 312, 489, 501
BssAI RCCGGY 2 cut(s) 252, 1178
BssECI CCNNGG 5 cut(s) 495, 1078, 1241, 1381, 1482
BssMI GATC 5 cut(s) 87, 324, 747, 1270, 1549
BssT1I CCWWGG 4 cut(s) 495, 1078, 1241, 1381
Bst2UI CCWGG 2 cut(s) 124, 1554
Bst4CI ACNGT 4 cut(s) 107, 285, 509, 1259
Bst6I CTCTTC 1 cut(s) 795
BstAPI GCANNNNNTGC 1 cut(s) 449
BstDEI CTNAG 6 cut(s) 321, 374, 408, 813, 1098, 1107
BstDSI CCRYGG 3 cut(s) 495, 1241, 1482
BstEII GGTNACC 1 cut(s) 835
BstF5I GGATG 3 cut(s) 835, 1023, 1251
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 2 cut(s) 175, 1356
BstKTI GATC 5 cut(s) 90, 327, 750, 1273, 1552
BstMAI GTCTC 4 cut(s) 113, 322, 508, 1443
BstMBI GATC 5 cut(s) 87, 324, 747, 1270, 1549
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 9 cut(s) 53, 62, 245, 449, 1069, 1360, 1376, 1397, 1484
BstNI CCWGG 2 cut(s) 124, 1554
BstNSI RCATGY 7 cut(s) 252, 664, 970, 974, 1327, 1360, 1374
BstPAI GACNNNNGTC 1 cut(s) 1462
BstPI GGTNACC 1 cut(s) 835
BstSCI CCNGG 2 cut(s) 122, 1552
BstSFI CTRYAG 2 cut(s) 230, 1168
BstV1I GCAGC 3 cut(s) 455, 1182, 1347
BstV2I GAAGAC 1 cut(s) 1138
BstX2I RGATCY 1 cut(s) 1549
BstXI CCANNNNNNTGG 1 cut(s) 496
BstYI RGATCY 1 cut(s) 1549
BsuRI GGCC 6 cut(s) 152, 500, 569, 1077, 1435, 1487
BtgI CCRYGG 3 cut(s) 495, 1241, 1482
BtgZI GCGATG 1 cut(s) 1082
BtsCI GGATG 3 cut(s) 835, 1023, 1251
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 4 cut(s) 75, 496, 508, 956
CciI TCATGA 1 cut(s) 628
CfoI GCGC 2 cut(s) 175, 1356
Cfr10I RCCGGY 2 cut(s) 252, 1178
Cfr13I GGNCC 1 cut(s) 1433
DdeI CTNAG 6 cut(s) 321, 374, 408, 813, 1098, 1107
DpnI GATC 5 cut(s) 89, 326, 749, 1272, 1551
DpnII GATC 5 cut(s) 87, 324, 747, 1270, 1549
EaeI YGGCCR 3 cut(s) 150, 498, 1485
Eam1104I CTCTTC 1 cut(s) 795
EarI CTCTTC 1 cut(s) 795
Eco130I CCWWGG 4 cut(s) 495, 1078, 1241, 1381
Eco147I AGGCCT 1 cut(s) 569
Eco31I GGTCTC 1 cut(s) 113
Eco47III AGCGCT 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 797
Eco88I CYCGRG 1 cut(s) 887
Eco91I GGTNACC 1 cut(s) 835
EcoO109I RGGNCCY 1 cut(s) 1433
EcoO65I GGTNACC 1 cut(s) 835
EcoRI GAATTC 1 cut(s) 430
EcoRII CCWGG 2 cut(s) 122, 1552
EcoT14I CCWWGG 4 cut(s) 495, 1078, 1241, 1381
EcoT22I ATGCAT 1 cut(s) 972
ErhI CCWWGG 4 cut(s) 495, 1078, 1241, 1381
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 428
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 1 cut(s) 448
FbaI TGATCA 1 cut(s) 747
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 4 cut(s) 444, 1171, 1361, 1479
FokI GGATG 3 cut(s) 842, 1030, 1258
Fsp4HI GCNGC 4 cut(s) 444, 1171, 1361, 1479
FspBI CTAG 5 cut(s) 693, 1064, 1088, 1392, 1397
GlaI GCGC 2 cut(s) 174, 1355
GluI GCNGC 4 cut(s) 444, 1171, 1361, 1479
GsuI CTGGAG 1 cut(s) 230
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 6 cut(s) 152, 500, 569, 1077, 1435, 1487
HapII CCGG 5 cut(s) 153, 253, 827, 1022, 1179
HhaI GCGC 2 cut(s) 175, 1356
Hin6I GCGC 2 cut(s) 173, 1354
HinP1I GCGC 2 cut(s) 173, 1354
HindIII AAGCTT 2 cut(s) 877, 996
HinfI GANTC 2 cut(s) 1402, 1536
HpaII CCGG 5 cut(s) 153, 253, 827, 1022, 1179
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 4 cut(s) 7, 103, 643, 1189
Hpy188I TCNGA 7 cut(s) 140, 322, 348, 411, 777, 1110, 1407
Hpy188III TCNNGA 7 cut(s) 110, 209, 272, 620, 629, 706, 991
Hpy8I GTNNAC 4 cut(s) 7, 103, 643, 1189
HpyAV CCTTC 8 cut(s) 23, 269, 353, 577, 626, 772, 1077, 1525
HpyCH4III ACNGT 4 cut(s) 107, 285, 509, 1259
HpyCH4IV ACGT 1 cut(s) 979
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 62, 245, 449, 1069, 1360, 1376, 1397, 1484
HpyF3I CTNAG 6 cut(s) 321, 374, 408, 813, 1098, 1107
HpySE526I ACGT 1 cut(s) 979
HspAI GCGC 2 cut(s) 173, 1354
KroI GCCGGC 1 cut(s) 252
KroNI GCCGGC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 5 cut(s) 87, 324, 747, 1270, 1549
LmnI GCTCC 1 cut(s) 1249
Lsp1109I GCAGC 3 cut(s) 455, 1182, 1347
LweI GCATC 4 cut(s) 702, 1008, 1059, 1101
MaeI CTAG 5 cut(s) 693, 1064, 1088, 1392, 1397
MaeII ACGT 1 cut(s) 979
MaeIII GTNAC 3 cut(s) 285, 503, 835
MalI GATC 5 cut(s) 89, 326, 749, 1272, 1551
MboI GATC 5 cut(s) 87, 324, 747, 1270, 1549
MboII GAAGA 3 cut(s) 210, 812, 1138
MflI RGATCY 1 cut(s) 1549
MhlI GDGCHC 1 cut(s) 1254
MlsI TGGCCA 1 cut(s) 500
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 2 cut(s) 1411, 1545
Mox20I TGGCCA 1 cut(s) 500
Mph1103I ATGCAT 1 cut(s) 972
MroNI GCCGGC 1 cut(s) 252
MscI TGGCCA 1 cut(s) 500
MseI TTAA 5 cut(s) 243, 419, 527, 1128, 1209
MslI CAYNNNNRTG 2 cut(s) 597, 1422
Msp20I TGGCCA 1 cut(s) 500
MspI CCGG 5 cut(s) 153, 253, 827, 1022, 1179
MspR9I CCNGG 2 cut(s) 124, 1554
MvaI CCWGG 2 cut(s) 124, 1554
MwoI GCNNNNNNNGC 9 cut(s) 53, 62, 245, 449, 1069, 1360, 1376, 1397, 1484
NaeI GCCGGC 1 cut(s) 254
NcoI CCATGG 2 cut(s) 495, 1241
NdeI CATATG 1 cut(s) 448
NdeII GATC 5 cut(s) 87, 324, 747, 1270, 1549
NgoMIV GCCGGC 1 cut(s) 252
NheI GCTAGC 2 cut(s) 1063, 1087
NmeAIII GCCGAG 1 cut(s) 433
NmuCI GTSAC 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 972
NspI RCATGY 7 cut(s) 252, 664, 970, 974, 1327, 1360, 1374
PaeI GCATGC 3 cut(s) 252, 974, 1360
PagI TCATGA 1 cut(s) 628
PceI AGGCCT 1 cut(s) 569
PdiI GCCGGC 1 cut(s) 254
PkrI GCNGC 4 cut(s) 445, 1172, 1362, 1480
PleI GAGTC 2 cut(s) 1410, 1544
PpsI GAGTC 2 cut(s) 1410, 1544
PshAI GACNNNNGTC 1 cut(s) 1462
PsiI TTATAA 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 122, 1552
PspEI GGTNACC 1 cut(s) 835
PspGI CCWGG 2 cut(s) 122, 1552
PspPI GGNCC 1 cut(s) 1433
PstI CTGCAG 2 cut(s) 234, 1172
PsuI RGATCY 1 cut(s) 1549
RseI CAYNNNNRTG 2 cut(s) 597, 1422
SaqAI TTAA 5 cut(s) 243, 419, 527, 1128, 1209
SatI GCNGC 4 cut(s) 444, 1171, 1361, 1479
Sau3AI GATC 5 cut(s) 87, 324, 747, 1270, 1549
Sau96I GGNCC 1 cut(s) 1433
SchI GAGTC 2 cut(s) 1411, 1545
ScrFI CCNGG 2 cut(s) 124, 1554
SduI GDGCHC 1 cut(s) 1254
SfaNI GCATC 4 cut(s) 702, 1008, 1059, 1101
SfcI CTRYAG 2 cut(s) 230, 1168
SmiMI CAYNNNNRTG 2 cut(s) 597, 1422
SmlI CTYRAG 1 cut(s) 571
SmoI CTYRAG 1 cut(s) 571
SphI GCATGC 3 cut(s) 252, 974, 1360
SseBI AGGCCT 1 cut(s) 569
SsiI CCGC 3 cut(s) 63, 1446, 1479
SspMI CTAG 5 cut(s) 693, 1064, 1088, 1392, 1397
StuI AGGCCT 1 cut(s) 569
StyD4I CCNGG 2 cut(s) 122, 1552
StyI CCWWGG 4 cut(s) 495, 1078, 1241, 1381
TaaI ACNGT 4 cut(s) 107, 285, 509, 1259
TaiI ACGT 1 cut(s) 982
TaqI TCGA 3 cut(s) 111, 1466, 1569
TaqII GACCGA 1 cut(s) 111
TauI GCSGC 1 cut(s) 1481
Tru1I TTAA 5 cut(s) 243, 419, 527, 1128, 1209
Tru9I TTAA 5 cut(s) 243, 419, 527, 1128, 1209
TscAI CASTG 4 cut(s) 82, 496, 508, 963
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 3 cut(s) 443, 1170, 1360
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 6 cut(s) 27, 414, 581, 1250, 1427, 1538
TspRI CASTG 4 cut(s) 82, 496, 508, 963
XapI RAATTY 4 cut(s) 70, 430, 1005, 1468
XceI RCATGY 7 cut(s) 252, 664, 970, 974, 1327, 1360, 1374
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 5 cut(s) 693, 1064, 1088, 1392, 1397
Zsp2I ATGCAT 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.