MD15G1012900.v1.1

Belongs to the hexokinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011448
Physical Location & Seq
Reverse (-)
711749 .. 714569
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1012900.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGTCAGCTGCTACCACGTCCCCAGCCGTTCGATCTTTCTACCTCCGGCGCCGATCACCTACGGTGGCCATGCCTCGCTGCAGGATGGCCGTCCGATCCGCTGCCGTTTCCCTATCTCCGCTGTTGACCAAGCTACACAACGACAGTGCCACTCCCCTGCCCCTCCTGCGCCACGTGGCAGACTCCATGGCCTCCGACATGCGTGCCGGGCTCGCCCGGGACGGCGGCAGCGACCTCAAGATGATACTCAGCTACGTTGATTCCCTCCCAAGCGGGAATGAGGAGGGGTTGTTTTATGCACTGGATCTTGGAGGCACAAACTTCAGGGTGCTGAGAGTACAATTAGGCGGCAAAGATGAGCGCGTGATTGCAACCGAATTCGACCAAGTTTCCATCCCCCCGGAGCTCATGTGTGGCACCTCAGAGGAACTGTTTGATTTTATTGCTTCTGGTTTGGCAAAGTTTGCACAAAAGGAGGGTAATAAATTCCACCTTCCAACTGGTACGAAAAGAGAAATTGGATTCACATTTTCGTTCCCTGTGAAGCAGACCTCCATTGATTCCGGCATACTAATTAAGTGGACAAAAGGTTTTTCCGTATCTGGAACGGCAGGAAGAGATGTGGTTGCTTGTCTGAATGAGGCTATGGAAAGGAAGGGACTCGATATGCGCGTGTCTGCTCTGGTTAACGATACGGTAGGAACTTTAGCTGGAGCAAGATATTGGGACGATGATGTCATGGTTGCTGTCATTTTGGGTACTGGTACCAACGCTTGCTATGTTGAACGTGTAGATGCTATTCCCAAACTACAGGGCCAGGTTTCTTCTTCCGGCAGAACGATTATCAACACTGAGTGGGGAGCATTCTCAAATGGTCTTCCTTTGACGGAATTCGATAGAGATATGGATGCTGCTAGCATCAATCCCGGTGAGCAGATCTTTGAGAAAACAATCTCGGGAATGTACCTCGGGGAAATTGTAAGAAGAGTACTAGTAAAGATGGCTGAAGCAGATGCTTTGTTTGGTGAATCTGTCCTAGAAAAACTATCAACACCTTTTCTACTCAGGACTCCATATCTTTGCGCTATGCAGCAAGATGCATCTGATGACCTTCAAGCTGTTGGGTCAATCCTATACAATGTAGCAGGGGTTGAGTCCAGTTTAAGTGCAAGAAAGATTGTGGTGGATGTATGTGACACCATAGTGAAGCGAGGCGGACGCTTAGCAGGTGCAGGAATAGTAGGGATACTGCAAAAGATGGAGGAGGATTCAAAAGATCTCATTTTTGGCAAGAGGACTGTTGTGGCCATGGATGGAGGGCTGTACGAAAACTATCCACAATACCGGAGGTATTTACAAGAGGCGGTGGCAGAGCTTCTAGGGACAGAGATTTCGAAGAACGTGGTGATCGAGCATTCGAAAGACGGGTCTGGTATAGGGGCCGCTCTCTTGGCTGCTGCAAATTCCCAGTACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001678 GO:0003674 GO:0003824 GO:0004340 GO:0004396 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008865 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019158 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019725 GO:0019752 GO:0032787 GO:0033500 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042592 GO:0042593 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0048878 GO:0051156 GO:0051186 GO:0051188 GO:0055082 GO:0055086 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00052 ko00500 ko00520 ko00521 ko00524 ko01100 ko01110 ko01120 ko01130 ko01200 ko04066 ko04910 ko04930 ko04973 ko05230 map00010 map00051 map00052 map00500 map00520 map00521 map00524 map01100 map01110 map01120 map01130 map01200 map04066 map04910 map04930 map04973 map05230
Pfam Domains
Protein Families
Hexokinase_1 Hexokinase_2

Protein Analysis

493

Amino Acids

53.12

Weight (kDa)

5.43

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hexokinase_1 PF00349 44 - 241 8.6e-70 Hexokinase
Hexokinase_2 PF03727 248 - 486 1.2e-82 Hexokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011448)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47840 AT1G47840
fragaria_vesca FvH4_2g38530 FvH4_2g38530 FvH4_2g38530
malus_domestica MD15G1012900.v1.1
prunus_persica Prupe.1G366000_v2.0.a1 Prupe.1G366000_v2.0.a1
pyrus_communis pycom15g01120
rosa_chinensis RchiOBHm_Chr6g0302781
rosa_laevigata RLG00000011138
rosa_multiflora Rmu_co8398525.1_g000001 Rmu_ssc0000460.1_g000004
rosa_roxburghii Rroxscaffold_7G00165330
rosa_rugosa Rorug06G0316900
rosa_samantha Rh6AG430300 Rh6BG472300 Rh6CG442800 Rh6DG429500
rosa_wichuraiana Rw6G037270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1217
AasI GACNNNNNNGTC 1 cut(s) 734
Acc36I ACCTGC 1 cut(s) 1217
Acc65I GGTACC 1 cut(s) 764
AccB1I GGYRCC 3 cut(s) 48, 416, 764
AccBSI CCGCTC 1 cut(s) 1445
AccII CGCG 2 cut(s) 363, 672
AciI CCGC 8 cut(s) 99, 119, 225, 273, 348, 1215, 1364, 1443
AclWI GGATC 2 cut(s) 90, 312
AcoI YGGCCR 3 cut(s) 66, 87, 1305
AcsI RAATTY 4 cut(s) 377, 485, 890, 1462
AcuI CTGAAG 2 cut(s) 307, 1026
AcvI CACGTG 1 cut(s) 175
AcyI GRCGYC 1 cut(s) 49
AdeI CACNNNGTG 1 cut(s) 855
AfaI GTAC 8 cut(s) 339, 505, 760, 766, 965, 990, 1325, 1472
AflIII ACRYGT 1 cut(s) 787
AgsI TTSAA 3 cut(s) 785, 1115, 1272
AhlI ACTAGT 1 cut(s) 991
AjiI CACGTC 1 cut(s) 18
AjnI CCWGG 1 cut(s) 816
AleI CACNNNNGTG 1 cut(s) 1202
AluBI AGCT 7 cut(s) 8, 133, 252, 406, 710, 1118, 1375
AluI AGCT 7 cut(s) 8, 133, 252, 406, 710, 1118, 1375
Alw21I GWGCWC 1 cut(s) 408
AlwI GGATC 2 cut(s) 90, 312
Ama87I CYCGRG 3 cut(s) 216, 955, 968
AoxI GGCC 6 cut(s) 66, 87, 189, 814, 1305, 1440
ApeKI GCWGC 8 cut(s) 8, 78, 101, 228, 911, 1090, 1454, 1457
ApoI RAATTY 4 cut(s) 377, 485, 890, 1462
ArsI GACNNNNNNTTYG 4 cut(s) 1375, 1407, 1412, 1444
Asp718I GGTACC 1 cut(s) 764
AspLEI GCGC 5 cut(s) 51, 171, 363, 672, 1085
AspS9I GGNCC 2 cut(s) 814, 1440
AsuC2I CCSGG 5 cut(s) 208, 217, 218, 401, 927
AsuHPI GGTGA 4 cut(s) 48, 941, 1037, 1417
AsuII TTCGAA 2 cut(s) 1394, 1418
AsuNHI GCTAGC 1 cut(s) 914
AvaI CYCGRG 3 cut(s) 216, 955, 968
BalI TGGCCA 2 cut(s) 68, 1307
BanI GGYRCC 3 cut(s) 48, 416, 764
BanII GRGCYC 2 cut(s) 213, 408
BbrPI CACGTG 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 869
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 7 cut(s) 65, 88, 240, 898, 1102, 1441, 1444
BccI CCATC 5 cut(s) 79, 401, 994, 1252, 1307
BceAI ACGGC 5 cut(s) 11, 74, 89, 238, 624
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 1 cut(s) 818
BciVI GTATCC 1 cut(s) 1239
BcnI CCSGG 5 cut(s) 208, 217, 218, 401, 927
BcuI ACTAGT 1 cut(s) 991
BfaI CTAG 4 cut(s) 915, 992, 1037, 1379
BfmI CTRYAG 2 cut(s) 79, 809
BfoI RGCGCY 1 cut(s) 52
BfuAI ACCTGC 1 cut(s) 1217
BfuI GTATCC 1 cut(s) 1239
BglII AGATCT 2 cut(s) 936, 1276
BlpI GCTNAGC 1 cut(s) 1222
BmcAI AGTACT 1 cut(s) 990
Bme1390I CCNGG 6 cut(s) 208, 217, 218, 401, 818, 927
BmeT110I CYCGRG 3 cut(s) 216, 955, 968
BmgBI CACGTC 1 cut(s) 18
BmgT120I GGNCC 2 cut(s) 814, 1440
BmiI GGNNCC 4 cut(s) 50, 418, 766, 1441
BmrFI CCNGG 6 cut(s) 208, 217, 218, 401, 818, 927
BmrI ACTGGG 1 cut(s) 1462
BmsI GCATC 6 cut(s) 784, 898, 927, 1003, 1087, 1109
BmtI GCTAGC 1 cut(s) 918
BmuI ACTGGG 1 cut(s) 1462
BpiI GAAGAC 1 cut(s) 869
BpmI CTGGAG 1 cut(s) 732
Bpu1102I GCTNAGC 1 cut(s) 1222
Bpu14I TTCGAA 2 cut(s) 1394, 1418
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 5 cut(s) 208, 217, 218, 401, 927
BsaAI YACGTR 1 cut(s) 175
BsaHI GRCGYC 1 cut(s) 49
BsaJI CCNNGG 5 cut(s) 186, 216, 399, 967, 1308
BsaWI WCCGGW 1 cut(s) 1344
BsaXI ACNNNNNCTCC 4 cut(s) 275, 305, 395, 425
Bse1I ACTGG 5 cut(s) 306, 505, 766, 1158, 1468
BseBI CCWGG 1 cut(s) 818
BseDI CCNNGG 5 cut(s) 186, 216, 399, 967, 1308
BseGI GGATG 5 cut(s) 90, 393, 913, 1192, 1318
BseMII CTCAG 5 cut(s) 262, 323, 435, 843, 1078
BseNI ACTGG 5 cut(s) 306, 505, 766, 1158, 1468
BseRI GAGGAG 2 cut(s) 296, 1277
BseXI GCAGC 7 cut(s) 65, 88, 240, 898, 1102, 1441, 1444
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 1 cut(s) 1251
Bsh1236I CGCG 2 cut(s) 363, 672
BshFI GGCC 6 cut(s) 68, 89, 191, 816, 1307, 1442
BshNI GGYRCC 3 cut(s) 48, 416, 764
BsiHKAI GWGCWC 1 cut(s) 408
BsiHKCI CYCGRG 3 cut(s) 216, 955, 968
BsiSI CCGG 8 cut(s) 46, 207, 217, 401, 564, 831, 927, 1345
BslFI GGGAC 5 cut(s) 4, 233, 672, 740, 1396
BsmFI GGGAC 5 cut(s) 4, 233, 672, 740, 1396
BsmI GAATGC 2 cut(s) 863, 1414
BsnI GGCC 6 cut(s) 68, 89, 191, 816, 1307, 1442
BsoBI CYCGRG 3 cut(s) 216, 955, 968
Bsp119I TTCGAA 2 cut(s) 1394, 1418
Bsp1286I GDGCHC 2 cut(s) 213, 408
Bsp143I GATC 7 cut(s) 32, 53, 95, 304, 936, 1276, 1407
Bsp1720I GCTNAGC 1 cut(s) 1222
Bsp19I CCATGG 2 cut(s) 186, 1308
BspACI CCGC 8 cut(s) 99, 119, 225, 273, 348, 1215, 1364, 1443
BspANI GGCC 6 cut(s) 68, 89, 191, 816, 1307, 1442
BspCNI CTCAG 5 cut(s) 261, 324, 434, 844, 1077
BspFNI CGCG 2 cut(s) 363, 672
BspLI GGNNCC 4 cut(s) 50, 418, 766, 1441
BspMAI CTGCAG 1 cut(s) 83
BspMI ACCTGC 1 cut(s) 1217
BspOI GCTAGC 1 cut(s) 918
BspPI GGATC 2 cut(s) 90, 312
BspT104I TTCGAA 2 cut(s) 1394, 1418
BspT107I GGYRCC 3 cut(s) 48, 416, 764
BsrBI CCGCTC 1 cut(s) 1445
BsrI ACTGG 5 cut(s) 306, 505, 766, 1158, 1468
BssECI CCNNGG 5 cut(s) 186, 216, 399, 967, 1308
BssMI GATC 7 cut(s) 32, 53, 95, 304, 936, 1276, 1407
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 2 cut(s) 186, 1308
Bst2UI CCWGG 1 cut(s) 818
Bst4CI ACNGT 5 cut(s) 64, 146, 432, 697, 1300
Bst6I CTCTTC 2 cut(s) 610, 979
BstACI GRCGYC 1 cut(s) 49
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBAI YACGTR 1 cut(s) 175
BstBI TTCGAA 2 cut(s) 1394, 1418
BstC8I GCNNGC 4 cut(s) 204, 213, 775, 916
BstDEI CTNAG 6 cut(s) 248, 332, 421, 852, 1064, 1222
BstDSI CCRYGG 2 cut(s) 186, 1308
BstF5I GGATG 5 cut(s) 90, 393, 913, 1192, 1318
BstFNI CGCG 2 cut(s) 363, 672
BstH2I RGCGCY 1 cut(s) 52
BstHHI GCGC 5 cut(s) 51, 171, 363, 672, 1085
BstKTI GATC 7 cut(s) 35, 56, 98, 307, 939, 1279, 1410
BstMBI GATC 7 cut(s) 32, 53, 95, 304, 936, 1276, 1407
BstMWI GCNNNNNNNGC 5 cut(s) 166, 208, 212, 464, 1451
BstNI CCWGG 1 cut(s) 818
BstNSI RCATGY 1 cut(s) 202
BstSCI CCNGG 6 cut(s) 206, 215, 216, 399, 816, 925
BstSFI CTRYAG 2 cut(s) 79, 809
BstUI CGCG 2 cut(s) 363, 672
BstV1I GCAGC 7 cut(s) 65, 88, 240, 898, 1102, 1441, 1444
BstV2I GAAGAC 1 cut(s) 869
BstX2I RGATCY 3 cut(s) 304, 936, 1276
BstYI RGATCY 3 cut(s) 304, 936, 1276
BsuI GTATCC 1 cut(s) 1239
BsuRI GGCC 6 cut(s) 68, 89, 191, 816, 1307, 1442
BtgI CCRYGG 2 cut(s) 186, 1308
BtrI CACGTC 1 cut(s) 18
BtsCI GGATG 5 cut(s) 90, 393, 913, 1192, 1318
BtsIMutI CAGTG 3 cut(s) 151, 299, 849
BveI ACCTGC 1 cut(s) 1217
Cac8I GCNNGC 4 cut(s) 204, 213, 775, 916
CfoI GCGC 5 cut(s) 51, 171, 363, 672, 1085
Cfr13I GGNCC 2 cut(s) 814, 1440
Cfr9I CCCGGG 1 cut(s) 216
CseI GACGC 1 cut(s) 1227
Csp6I GTAC 8 cut(s) 338, 504, 759, 765, 964, 989, 1324, 1471
CviAII CATG 6 cut(s) 70, 187, 199, 409, 739, 1309
CviQI GTAC 8 cut(s) 338, 504, 759, 765, 964, 989, 1324, 1471
DdeI CTNAG 6 cut(s) 248, 332, 421, 852, 1064, 1222
DinI GGCGCC 1 cut(s) 50
DpnI GATC 7 cut(s) 34, 55, 97, 306, 938, 1278, 1409
DpnII GATC 7 cut(s) 32, 53, 95, 304, 936, 1276, 1407
DraIII CACNNNGTG 1 cut(s) 855
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 3 cut(s) 66, 87, 1305
Eam1104I CTCTTC 2 cut(s) 610, 979
EarI CTCTTC 2 cut(s) 610, 979
EciI GGCGGA 1 cut(s) 1230
Ecl136II GAGCTC 1 cut(s) 406
Eco130I CCWWGG 2 cut(s) 186, 1308
Eco24I GRGCYC 2 cut(s) 213, 408
Eco53kI GAGCTC 1 cut(s) 406
Eco57I CTGAAG 2 cut(s) 307, 1026
Eco72I CACGTG 1 cut(s) 175
Eco88I CYCGRG 3 cut(s) 216, 955, 968
EcoICRI GAGCTC 1 cut(s) 406
EcoRI GAATTC 2 cut(s) 377, 890
EcoRII CCWGG 1 cut(s) 816
EcoT14I CCWWGG 2 cut(s) 186, 1308
EcoT22I ATGCAT 1 cut(s) 1102
EcoT38I GRGCYC 2 cut(s) 213, 408
EgeI GGCGCC 1 cut(s) 50
EheI GGCGCC 1 cut(s) 50
ErhI CCWWGG 2 cut(s) 186, 1308
FaeI CATG 6 cut(s) 73, 190, 202, 412, 742, 1312
FaqI GGGAC 5 cut(s) 4, 233, 672, 740, 1396
FatI CATG 6 cut(s) 69, 186, 198, 408, 738, 1308
FauI CCCGC 1 cut(s) 266
FauNDI CATATG 1 cut(s) 1475
FokI GGATG 5 cut(s) 97, 380, 920, 1199, 1325
FriOI GRGCYC 2 cut(s) 213, 408
FspBI CTAG 4 cut(s) 915, 992, 1037, 1379
GlaI GCGC 5 cut(s) 50, 170, 362, 671, 1084
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 732
HaeII RGCGCY 1 cut(s) 52
HaeIII GGCC 6 cut(s) 68, 89, 191, 816, 1307, 1442
HapII CCGG 8 cut(s) 46, 207, 217, 401, 564, 831, 927, 1345
HgaI GACGC 1 cut(s) 1227
HhaI GCGC 5 cut(s) 51, 171, 363, 672, 1085
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 6 cut(s) 73, 190, 202, 412, 742, 1312
Hin6I GCGC 5 cut(s) 49, 169, 361, 670, 1083
HinP1I GCGC 5 cut(s) 49, 169, 361, 670, 1083
HincII GTYRAC 2 cut(s) 126, 688
HindII GTYRAC 2 cut(s) 126, 688
HinfI GANTC 9 cut(s) 182, 260, 522, 560, 660, 1028, 1069, 1154, 1268
HpaI GTTAAC 1 cut(s) 688
HpaII CCGG 8 cut(s) 46, 207, 217, 401, 564, 831, 927, 1345
HphI GGTGA 4 cut(s) 48, 941, 1037, 1417
Hpy166II GTNNAC 3 cut(s) 126, 582, 688
Hpy188I TCNGA 5 cut(s) 95, 196, 424, 636, 1105
Hpy188III TCNNGA 4 cut(s) 238, 603, 957, 1066
Hpy8I GTNNAC 3 cut(s) 126, 582, 688
HpyAV CCTTC 3 cut(s) 503, 649, 1121
HpyCH4III ACNGT 5 cut(s) 64, 146, 432, 697, 1300
HpyCH4IV ACGT 5 cut(s) 17, 174, 255, 787, 1401
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 208, 212, 464, 1451
HpyF3I CTNAG 6 cut(s) 248, 332, 421, 852, 1064, 1222
HpySE526I ACGT 5 cut(s) 17, 174, 255, 787, 1401
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 6 cut(s) 73, 190, 202, 412, 742, 1312
HspAI GCGC 5 cut(s) 49, 169, 361, 670, 1083
KasI GGCGCC 1 cut(s) 48
KpnI GGTACC 1 cut(s) 768
KspAI GTTAAC 1 cut(s) 688
Kzo9I GATC 7 cut(s) 32, 53, 95, 304, 936, 1276, 1407
LmnI GCTCC 3 cut(s) 403, 713, 860
Lsp1109I GCAGC 7 cut(s) 65, 88, 240, 898, 1102, 1441, 1444
LweI GCATC 6 cut(s) 784, 898, 927, 1003, 1087, 1109
MaeI CTAG 4 cut(s) 915, 992, 1037, 1379
MaeII ACGT 5 cut(s) 17, 174, 255, 787, 1401
MaeIII GTNAC 1 cut(s) 1193
MalI GATC 7 cut(s) 34, 55, 97, 306, 938, 1278, 1409
MbiI CCGCTC 1 cut(s) 1445
MboI GATC 7 cut(s) 32, 53, 95, 304, 936, 1276, 1407
MboII GAAGA 6 cut(s) 627, 816, 819, 869, 996, 1408
MflI RGATCY 3 cut(s) 304, 936, 1276
MhlI GDGCHC 2 cut(s) 213, 408
MlsI TGGCCA 2 cut(s) 68, 1307
MluCI AATT 8 cut(s) 341, 377, 485, 516, 573, 890, 975, 1462
MluNI TGGCCA 2 cut(s) 68, 1307
Mly113I GGCGCC 1 cut(s) 49
MlyI GAGTC 4 cut(s) 176, 654, 1063, 1163
MmeI TCCRAC 2 cut(s) 219, 521
Mox20I TGGCCA 2 cut(s) 68, 1307
Mph1103I ATGCAT 1 cut(s) 1102
MscI TGGCCA 2 cut(s) 68, 1307
MseI TTAA 3 cut(s) 576, 687, 1163
MslI CAYNNNNRTG 1 cut(s) 1202
Msp20I TGGCCA 2 cut(s) 68, 1307
MspA1I CMGCKG 3 cut(s) 8, 101, 121
MspI CCGG 8 cut(s) 46, 207, 217, 401, 564, 831, 927, 1345
MspR9I CCNGG 6 cut(s) 208, 217, 218, 401, 818, 927
Mva1269I GAATGC 2 cut(s) 863, 1414
MvaI CCWGG 1 cut(s) 818
MvnI CGCG 2 cut(s) 363, 672
MwoI GCNNNNNNNGC 5 cut(s) 166, 208, 212, 464, 1451
NarI GGCGCC 1 cut(s) 49
NciI CCSGG 5 cut(s) 208, 217, 218, 401, 927
NcoI CCATGG 2 cut(s) 186, 1308
NdeI CATATG 1 cut(s) 1475
NdeII GATC 7 cut(s) 32, 53, 95, 304, 936, 1276, 1407
NheI GCTAGC 1 cut(s) 914
NlaIII CATG 6 cut(s) 73, 190, 202, 412, 742, 1312
NlaIV GGNNCC 4 cut(s) 50, 418, 766, 1441
NmuCI GTSAC 1 cut(s) 1193
NsiI ATGCAT 1 cut(s) 1102
NspI RCATGY 1 cut(s) 202
NspV TTCGAA 2 cut(s) 1394, 1418
OliI CACNNNNGTG 1 cut(s) 1202
PaqCI CACCTGC 1 cut(s) 1217
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 669, 1407
PctI GAATGC 2 cut(s) 863, 1414
PfeI GAWTC 5 cut(s) 260, 522, 560, 1028, 1268
PleI GAGTC 4 cut(s) 176, 654, 1063, 1162
PluTI GGCGCC 1 cut(s) 52
PmaCI CACGTG 1 cut(s) 175
PmlI CACGTG 1 cut(s) 175
PpsI GAGTC 4 cut(s) 176, 654, 1063, 1162
Ppu21I YACGTR 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 816
PspCI CACGTG 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 1 cut(s) 816
PspN4I GGNNCC 4 cut(s) 50, 418, 766, 1441
PspPI GGNCC 2 cut(s) 814, 1440
PstI CTGCAG 1 cut(s) 83
PsuI RGATCY 3 cut(s) 304, 936, 1276
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 8 cut(s) 339, 505, 760, 766, 965, 990, 1325, 1472
RsaNI GTAC 8 cut(s) 338, 504, 759, 765, 964, 989, 1324, 1471
RseI CAYNNNNRTG 1 cut(s) 1202
SacI GAGCTC 1 cut(s) 408
SaqAI TTAA 3 cut(s) 576, 687, 1163
Sau3AI GATC 7 cut(s) 32, 53, 95, 304, 936, 1276, 1407
Sau96I GGNCC 2 cut(s) 814, 1440
ScaI AGTACT 1 cut(s) 990
SchI GAGTC 4 cut(s) 176, 654, 1063, 1163
ScrFI CCNGG 6 cut(s) 208, 217, 218, 401, 818, 927
SduI GDGCHC 2 cut(s) 213, 408
SfaNI GCATC 6 cut(s) 784, 898, 927, 1003, 1087, 1109
SfcI CTRYAG 2 cut(s) 79, 809
SfoI GGCGCC 1 cut(s) 50
SfuI TTCGAA 2 cut(s) 1394, 1418
SmaI CCCGGG 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 1202
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SpeI ACTAGT 1 cut(s) 991
Sse9I AATT 8 cut(s) 341, 377, 485, 516, 573, 890, 975, 1462
SsiI CCGC 8 cut(s) 99, 119, 225, 273, 348, 1215, 1364, 1443
SspDI GGCGCC 1 cut(s) 48
SspMI CTAG 4 cut(s) 915, 992, 1037, 1379
SstI GAGCTC 1 cut(s) 408
StyD4I CCNGG 6 cut(s) 206, 215, 216, 399, 816, 925
StyI CCWWGG 2 cut(s) 186, 1308
TaaI ACNGT 5 cut(s) 64, 146, 432, 697, 1300
TaiI ACGT 5 cut(s) 20, 177, 258, 790, 1404
TaqI TCGA 7 cut(s) 31, 381, 663, 894, 1394, 1410, 1418
TasI AATT 8 cut(s) 341, 377, 485, 516, 573, 890, 975, 1462
TatI WGTACW 3 cut(s) 337, 988, 1470
TauI GCSGC 3 cut(s) 228, 351, 1445
TfiI GAWTC 5 cut(s) 260, 522, 560, 1028, 1268
Tru1I TTAA 3 cut(s) 576, 687, 1163
Tru9I TTAA 3 cut(s) 576, 687, 1163
TscAI CASTG 3 cut(s) 151, 306, 856
TseFI GTSAC 1 cut(s) 1193
TseI GCWGC 8 cut(s) 8, 78, 101, 228, 911, 1090, 1454, 1457
Tsp45I GTSAC 1 cut(s) 1193
TspGWI ACGGA 2 cut(s) 586, 902
TspMI CCCGGG 1 cut(s) 216
TspRI CASTG 3 cut(s) 151, 306, 856
XapI RAATTY 4 cut(s) 377, 485, 890, 1462
XceI RCATGY 1 cut(s) 202
XcmI CCANNNNNNNNNTGG 1 cut(s) 497
XmaI CCCGGG 1 cut(s) 216
XspI CTAG 4 cut(s) 915, 992, 1037, 1379
ZrmI AGTACT 1 cut(s) 990
Zsp2I ATGCAT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.