Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0022342

Physical Location & Seq

Genomic Coordinates Forward (+)

4625398 .. 4632215

Gene Structure 6818 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1066500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4521 bp

ATGATGATGGCTTCCAGAACTCCTGGAACGCCAAGAGCTAAGAAGGAGAAGATTGTTGTTTTAGTGTGTTTAAGGCCTCTAAGCAAAAGGGAGGAACTTGCAAAAGAACAAGTTTCGTGGGATTGCCTAGGCGATAACACCATTGTTTATAAACCCCCGCCTCACGAACGTTCCGCTCAATCCACCTCGTTTACATTTGATAAAGTTTTCGGTCCTGGTTCAACCGAAGCAGTATATGAGGAAGGAGTGAGAAATGTTGCCTTGTCTTCTCTGATGGGAATTAACGCAACTATATTTGCTTATGGACAAAGTGGCAGTGGCAAGACATACACAATGAGACGAATAATGGAAAAAGCTGTGGATGATATCTACAACCATATGATAAAGACTCCAGAGAGAGAGTTTGCAATAAAAATTTCAGGACTTGAGATATACAATGAGAATGTCCGCGACTTGCTGAATACAGAATCTGTTGGTAATTTAAAGCTTTTAGATGATCCAGAGAAAGGAATCGTGGTTGAGAAATTGGTGGAGGAAGCTGCAAGCAGTCATCAGCACCTGAGAAGTTTAATCGGAATTTGCGAGGCTCAAAGAGAAGTTGGTGAAACTGCTCTAAACGAAAACAGTTCACGGTCTCATCAAATAATAAGGCTGACAATTGAAAGTACAGTCCGTGAAAGTTTAGACTGCAGGACGTCTTTCGTTGCAAGCCTGAATTTTGTAGACCTAGCTGGAAGCGAAAGAGCTCCAGATACGCATGCAGACGGTGTTAGGCTAAGGGAAGGCTGCCATATTAACCTTAGTTTGATGACACTTACTAACGTTATCAGAAAGCTCAGTGCTGGAAAATTAAGTGGTCCTATACCTTATCGAGACTCAAAGCTCACTCGCATATTGCAACATTCTCTTGGAGGAAACGCTCGCACTGCCATCATATGTACCTTGAGTCCAGCACTGAGCCACGCTGAACAATCTCGAAACACTCTCTTCTTTGCTACACAGGCAAAGGAGGTAACAAATGATGCCAGCGTGAACACGGTTGTATCAGACAAGCAGCTAGTCAAACACTTGCAAAAGCAAGTAGCTAAGCTGGAAGCAGAGCTGTACACCCTTTATCCATTGAGGGAAAGAATTCAGCAGATGGAAATGGAAATGGAGGATTTGAGAAGCGAAAGAGATTTGGCTCGATCTCAGGCGGATGAGTTACGCCATAAACTTGAGGTTCAGAGTTTATATCCATTGGAATTACCTCATCGCTCTGTGGACAACTATCTCTCCTACACTGGTGTGCTACCAACATCATTTTATGCCAAGGAACTAGGAGATTGTGATACAGCAACAAATGCAAGGACCAAGCACTCCATTAGGCACTCGGCAACTCCTCCTTTCATGCCAAGACGTGAAGTTCTAAAACATGAAATCTCATCAGAGCAACTTTCGGAGGAACCAAGTCGAGCTCTAAAACTAGACGTGCTTTACAAAAGCAAATTGGTGATTGGAGGATTTAGCTTTCAAAATTTAGCTTTCAGGTGCTTAGAGGATTTATTTCGTCCTCAAATAAACTCTCTCTTACTTTCCACACAGGCAAAGGGTGAATCTAACATGGTTGTTTCGGACAAGAATCTAGTTAAACGTTTGCAAATGGAAGTGCTCAGGCGGGAAACAGAGGTACCCACCGTGTCAAGGGAAGAAGATTTAAGAATTCAGAAGGTTTTAAACCAACTGGAATTACCGCATCCTTATGTAAAGAAACTTATCTTCTGCATCGGTGTGCTACCTGCAACATTTTATGCTGAGGAGCTAGGAGGCGGTGATAGGGCAAGAAATACAATGATCAGGCACTCCATGAGGCTCTCATCATTTGCTCCTTTTACGCTAAACCATGAAATTCTAAGACTTGAAGTCCTGCTGGTGCAACTTGGGGAGGGACCAAGTCGAGCATTGGAAATACTAATACAGAAGGAGGTACTAGAAAACCATGATAGGGCAAGAAATACAATGATCAGGCACCCCATGAGGCTCTCATCATTTGCTCCTTTCATGCTAAACCGTGAAATTCTTAAACTTGAAGTCTTGCTGGCTCAACTTGGGGAGGGACCAAGTCGGGCTTTGGAAATAGTATTACAAAAGGAGGTAGCCTGGCAGGGACTAGAAAACCAAGATGCAGGTGAAACTTTTTGTAACCTGCAAGCAGAGATAAAAGAAACGCATTATACCAGTTCTCGGCCAAAAGAATTTGAAGTAGGAAGTCTTGTTCCTGTTAATAAAAAAGAAATTCCTAGACTTCACTCACAGGGCAGCGCCATTGCAAATCTTGAAGAACAGTTTCCAAAGGTGCAGTATTTTACAGACAAATTAGTGTCTCTCCCTTGCATTCAATTGTTGGTTTTGAGGTTCTTGGAGGATTTATTTCGTCCTCGTGAGGTTACTGGTGATGTTTTGGAACAAATTAAGAACGAGCCTGTAAAACATAACATAGTTCCGTTGCTCGGATGTTGGCAAGAAGATGAAGCCGAAGTTGATGGTGGCTTTCATCTTGAACCAGGAACATCAAATGCTGCTTCTCCAGATGTATCTGCTGTAGAATATGACCATATCAGTGAACATGTTTTTCATGTTAAAGTAGAACATCCTGCAGATAGATATGAGAAGAAGGCTATTTCTGATGAAGCCAAAGAGCTCATGGATTGTCATAGGGAGACAAGTGACAGCGGTGCATTCCGTAATGGGAAGATTGATGTCTCCGAAAATCGTGATTTCTTGGGCTTCCTATGTAGCAATGGTGGTCAGCTGCTGATTTGTAATACAAGTAATTGCCCTTTCGTATTTCATCAGAACTTCTTGGATTCTGACTTAATATGCTATAAGAAGGGTAAATATTACTGCCCCATCTGTTCATATTCTCTTGCTTATGAGAATAGGCTGGGTGAGATAGAAGAAAAGTTGAGAAGAAAGGCAGTGGCTTTTGATGAAGTCTTCTCCGGCTGTACTGATGGTTGTACCGTTAATGAACAAATGAATGCGCGAAATGAAAATGGTTATGGTTGTGACTGTCAATTTGACGATGCTCAAGTTCATAACCAATTTGTCGGAGTGGAAGACAATGAGATCAAAGAAGGGACGGAGTTTGAGGTTGAAGATGAGAACAATGGTGGCAACAGTGTCAGTTGTAGAGGTGAAGGCACCAATGAAATAATTAGAGGAGGTTCTGGACACCCATGCGTATCTGAACTGCTTGAGTTGCAACAGGATGAGGAAACTGCTGTCGAAGAAAATTCAGGCGAAAATGAAGAAGATGAAGGTGGGAAAAGGGTGTTTGTGGAGAAAGAAGACGAAATTGAGAATGCAGAACTTATGCAACCAGAATCAATGGAATTGCCCTCAAATACCATTGTTCTATCTGACACGGAGTCTCCTTTAGTGCATAAAAGAAGGGAAGACAATGAGATCAAGGAAGGGATGAAGTTTGAGGTTGAAGATGAGAACAATGATGGTGACAGTGTCAGTTGTAGAGGTGAAGGCACCAATGAAGTAATTAGAGGAGATTCGGAACACCCGTGCCTATCTGAACTGCTTGAGTTGCAACAGGATGAGGAAACTGCTGTCGAAGAAAATTCAGACAAAAATGAAGAAGATGAAGGTGGGAAAAAGGTGTTTGCGGAGAAAGAAGACGAAATTGAGAATGCAGAACTTATGCAACCAGAATCAATGGAAGTGCCCTTAAATACCATTGTTCTATCTGACACAGAGTCTCCTGTAGTGCATAAAAGAAGGGAAGACAATGAGATCAAAGAAGGGATGAAGTTTGAGGTTGAAGATGAGAACAATGATAGTGACAATGTCAGTTGTAGAGGTGAAGGCACCAATGAAGTAATTAGAGGAGATTCAGAACACCCGTGCGTATCTGAACTGCTTGAGTTGCAACAGGATGAGGAAACTGCTGTTGAAGAAAATTCAGACAAAAATGAAGAAGATGAAGGTGGGAAAAGGGTGTTTGCGGAGACAGAAAACGAAATTAAGAATGCAGAACTTATGCAACCAGAATCTCTGGAAATGCCCTCAAATACCATTGTTCTATCTGACACAGAGTCTCCTGTAGTGCATAAAAACCATGAAAAAGCGCCAAAGAGTTTTACCATCGTTCTGTCTGATTCCTCGGAATCTGATGCTTCAGATACTTGGTCTCCTTCCATGGAACCGGAAGTTTCAGATACTCGGCCTCCTCTACTGGGCGAGATACAGCCTAAACATAAAGGTCAAAAGACAAGAGAATTCGGAAATCAACCAACGAGCTCAAACTTGCCACTGCCTAGGACGAGGCGGCGTATGAGATTATACTGGACAAGAGAAGAAGAAGAAAAGTTGAAGGAGCTTGTGAACAGTTTTCCAACCACTGGAAAGTACCGATGCTGGAAGAAAATTTTGGAAAATGGTCGCGATGTATTTCACAAGGATCGAACTCCGAATGATCTGAGAAATAAATGGGGAAAGATGCGGGTGAAAGGCAATGCTGAATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000775 GO:0000910 GO:0000911 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007275 GO:0007349 GO:0008150 GO:0009524 GO:0009553 GO:0009555 GO:0009558 GO:0009561 GO:0009653 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022607 GO:0032501 GO:0032502 GO:0032506 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043515 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0048229 GO:0048285 GO:0048646 GO:0048856 GO:0051225 GO:0051301 GO:0061640 GO:0070925 GO:0071840 GO:0090307 GO:0098687 GO:0140014 GO:1902410 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3490 Kinesin

Protein Analysis

1507

Amino Acids

170.17

Weight (kDa)

4.98

Isoelectric Point (pI)

47.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	24 - 338	5.1e-92	Kinesin motor domain
Microtub_bd	PF16796	55 - 153	8.8e-20	Microtubule binding
Myb_DNA-binding	PF00249	1445 - 1497	5.7e-06	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022342)

Species	Orthologous Gene IDs
malus_domestica	MD15G1066500.v1.1
pyrus_communis	pycom15g06280 pycom15g06290
rosa_roxburghii	Rroxscaffold_3G00234200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	150
AarI	CACCTGC	1 cut(s)	2156
AatII	GACGTC	1 cut(s)	698
Acc36I	ACCTGC	3 cut(s)	1786, 2156, 2193
Acc65I	GGTACC	1 cut(s)	1669
AccB1I	GGYRCC	5 cut(s)	1669, 2007, 3182, 3521, 3860
AccB7I	CCANNNNNTGG	1 cut(s)	4394
AccBSI	CCGCTC	1 cut(s)	176
AccI	GTMKAC	1 cut(s)	723
AccII	CGCG	3 cut(s)	450, 3025, 4437
AclI	AACGTT	3 cut(s)	169, 822, 1633
AclWI	GGATC	2 cut(s)	491, 4461
AcoI	YGGCCR	1 cut(s)	2225
AcuI	CTGAAG	1 cut(s)	4155
AcyI	GRCGYC	1 cut(s)	695
AfaI	GTAC	8 cut(s)	667, 940, 1106, 1671, 1968, 2989, 3001, 4403
AfiI	CCNNNNNNNGG	7 cut(s)	506, 1683, 1984, 2223, 2489, 4229, 4394
AflIII	ACRYGT	1 cut(s)	2605
AhdI	GACNNNNNGTC	4 cut(s)	1901, 3409, 3748, 4087
AjiI	CACGTC	2 cut(s)	1400, 1471
AjnI	CCWGG	4 cut(s)	22, 214, 2138, 2542
AjuI	GAANNNNNNNTTGG	2 cut(s)	4406, 4438
AleI	CACNNNNGTG	1 cut(s)	1286
AloI	GAACNNNNNNTCC	2 cut(s)	2238, 2270
Alw21I	GWGCWC	5 cut(s)	748, 1459, 1653, 2682, 4295
AlwI	GGATC	2 cut(s)	491, 4461
AlwNI	CAGNNNCTG	3 cut(s)	470, 559, 2794
AoxI	GGCC	3 cut(s)	74, 2225, 4217
ApeKI	GCWGC	6 cut(s)	539, 786, 1054, 2298, 2558, 2791
ArsI	GACNNNNNNTTYG	2 cut(s)	3041, 3073
Asp700I	GAANNNNTTC	2 cut(s)	1131, 2325
Asp718I	GGTACC	1 cut(s)	1669
AspA2I	CCTAGG	2 cut(s)	127, 4310
AspLEI	GCGC	3 cut(s)	2303, 3025, 4123
AspS9I	GGNCC	5 cut(s)	212, 857, 1350, 1928, 2096
AvaII	GGWCC	5 cut(s)	212, 857, 1350, 1928, 2096
AvrII	CCTAGG	2 cut(s)	127, 4310
BaeGI	GKGCMC	1 cut(s)	3720
BaeI	ACNNNNGTAYC	4 cut(s)	1026, 1059, 1653, 1686
BanI	GGYRCC	5 cut(s)	1669, 2007, 3182, 3521, 3860
BanII	GRGCYC	4 cut(s)	748, 1459, 2682, 4295
BauI	CACGAG	1 cut(s)	2418
BbsI	GAAGAC	7 cut(s)	258, 2968, 3105, 3336, 3444, 3675, 3783
Bbv12I	GWGCWC	5 cut(s)	748, 1459, 1653, 2682, 4295
BbvCI	CCTCAGC	1 cut(s)	1794
BbvI	GCAGC	6 cut(s)	526, 773, 1066, 2310, 2545, 2778
BccI	CCATC	8 cut(s)	268, 938, 1135, 2516, 2897, 2987, 3485, 4145
BciT130I	CCWGG	4 cut(s)	24, 216, 2140, 2544
BclI	TGATCA	2 cut(s)	1833, 2001
BfmI	CTRYAG	5 cut(s)	688, 2580, 2634, 3756, 4095
BfoI	RGCGCY	2 cut(s)	2304, 4124
BfuAI	ACCTGC	3 cut(s)	1786, 2156, 2193
BisI	GCNGC	7 cut(s)	540, 787, 1055, 2299, 2559, 2792, 4322
BlnI	CCTAGG	2 cut(s)	127, 4310
BlpI	GCTNAGC	1 cut(s)	1086
BlsI	GCNGC	7 cut(s)	541, 788, 1056, 2300, 2560, 2793, 4323
Bme1390I	CCNGG	4 cut(s)	24, 216, 2140, 2544
Bme18I	GGWCC	5 cut(s)	212, 857, 1350, 1928, 2096
BmeRI	GACNNNNNGTC	4 cut(s)	1901, 3409, 3748, 4087
BmgBI	CACGTC	2 cut(s)	1400, 1471
BmgT120I	GGNCC	5 cut(s)	212, 857, 1350, 1928, 2096
BmiI	GGNNCC	9 cut(s)	1446, 1671, 1929, 2009, 2097, 3184, 3523, 3862, 4197
BmrFI	CCNGG	4 cut(s)	24, 216, 2140, 2544
BmrI	ACTGGG	1 cut(s)	4238
BmsI	GCATC	8 cut(s)	1012, 1744, 1773, 2152, 3055, 4156, 4397, 4482
BmuI	ACTGGG	1 cut(s)	4238
BpiI	GAAGAC	7 cut(s)	258, 2968, 3105, 3336, 3444, 3675, 3783
BpmI	CTGGAG	3 cut(s)	375, 732, 2550
Bpu10I	CCTNAGC	3 cut(s)	776, 1652, 1794
Bpu1102I	GCTNAGC	1 cut(s)	1086
BpuEI	CTTGAG	7 cut(s)	446, 964, 1238, 3054, 3257, 3596, 3935
BsaHI	GRCGYC	1 cut(s)	695
BsaI	GGTCTC	2 cut(s)	639, 4188
BsaJI	CCNNGG	5 cut(s)	127, 1311, 4155, 4191, 4310
BsaWI	WCCGGW	1 cut(s)	4198
Bsc4I	CCNNNNNNNGG	7 cut(s)	506, 1683, 1984, 2223, 2489, 4229, 4394
Bse1I	ACTGG	7 cut(s)	1288, 1728, 2217, 2434, 4233, 4343, 4399
Bse3DI	GCAATG	3 cut(s)	2304, 2785, 4513
BseBI	CCWGG	4 cut(s)	24, 216, 2140, 2544
BseDI	CCNNGG	5 cut(s)	127, 1311, 4155, 4191, 4310
BseLI	CCNNNNNNNGG	7 cut(s)	506, 1683, 1984, 2223, 2489, 4229, 4394
BseMI	GCAATG	3 cut(s)	2304, 2785, 4513
BseMII	CTCAG	7 cut(s)	551, 850, 947, 1205, 1666, 1785, 4463
BseNI	ACTGG	7 cut(s)	1288, 1728, 2217, 2434, 4233, 4343, 4399
BseRI	GAGGAG	6 cut(s)	1371, 1811, 3216, 3555, 3894, 4212
BseSI	GKGCMC	1 cut(s)	3720
BseXI	GCAGC	6 cut(s)	526, 773, 1066, 2310, 2545, 2778
BseYI	CCCAGC	1 cut(s)	2923
BsgI	GTGCAG	1 cut(s)	2357
Bsh1236I	CGCG	3 cut(s)	450, 3025, 4437
BshFI	GGCC	3 cut(s)	76, 2227, 4219
BshNI	GGYRCC	5 cut(s)	1669, 2007, 3182, 3521, 3860
BsiHKAI	GWGCWC	5 cut(s)	748, 1459, 1653, 2682, 4295
BsiSI	CCGG	2 cut(s)	2982, 4199
BslFI	GGGAC	4 cut(s)	1941, 2109, 2160, 3133
BslI	CCNNNNNNNGG	7 cut(s)	506, 1683, 1984, 2223, 2489, 4229, 4394
BsmBI	CGTCTC	1 cut(s)	331
BsmFI	GGGAC	4 cut(s)	1941, 2109, 2160, 3133
BsmI	GAATGC	6 cut(s)	2373, 2717, 3025, 3349, 3688, 4027
BsnI	GGCC	3 cut(s)	76, 2227, 4219
Bso31I	GGTCTC	2 cut(s)	639, 4188
Bsp1286I	GDGCHC	6 cut(s)	748, 1459, 1653, 2682, 3720, 4295
Bsp1407I	TGTACA	1 cut(s)	1104
Bsp143I	GATC	9 cut(s)	496, 1187, 1833, 2001, 3108, 3447, 3786, 4453, 4468
Bsp1720I	GCTNAGC	1 cut(s)	1086
Bsp19I	CCATGG	1 cut(s)	4191
Bsp68I	TCGCGA	1 cut(s)	4437
BspANI	GGCC	3 cut(s)	76, 2227, 4219
BspCNI	CTCAG	7 cut(s)	552, 849, 948, 1204, 1665, 1786, 4464
BspFNI	CGCG	3 cut(s)	450, 3025, 4437
BspLI	GGNNCC	9 cut(s)	1446, 1671, 1929, 2009, 2097, 3184, 3523, 3862, 4197
BspMAI	CTGCAG	2 cut(s)	692, 2638
BspMI	ACCTGC	3 cut(s)	1786, 2156, 2193
BspPI	GGATC	2 cut(s)	491, 4461
BspT107I	GGYRCC	5 cut(s)	1669, 2007, 3182, 3521, 3860
BspTNI	GGTCTC	2 cut(s)	639, 4188
BsrBI	CCGCTC	1 cut(s)	176
BsrDI	GCAATG	3 cut(s)	2304, 2785, 4513
BsrGI	TGTACA	1 cut(s)	1104
BsrI	ACTGG	7 cut(s)	1288, 1728, 2217, 2434, 4233, 4343, 4399
BssECI	CCNNGG	5 cut(s)	127, 1311, 4155, 4191, 4310
BssMI	GATC	9 cut(s)	496, 1187, 1833, 2001, 3108, 3447, 3786, 4453, 4468
BssNI	GRCGYC	1 cut(s)	695
BssSI	CACGAG	1 cut(s)	2418
BssT1I	CCWWGG	4 cut(s)	127, 1311, 4191, 4310
Bst2BI	CACGAG	1 cut(s)	2418
Bst2UI	CCWGG	4 cut(s)	24, 216, 2140, 2544
Bst6I	CTCTTC	1 cut(s)	992
BstACI	GRCGYC	1 cut(s)	695
BstAPI	GCANNNNNTGC	1 cut(s)	1343
BstAUI	TGTACA	1 cut(s)	1104
BstC8I	GCNNGC	7 cut(s)	544, 709, 759, 922, 1027, 2079, 2190
BstDSI	CCRYGG	1 cut(s)	4191
BstFNI	CGCG	3 cut(s)	450, 3025, 4437
BstH2I	RGCGCY	2 cut(s)	2304, 4124
BstHHI	GCGC	3 cut(s)	2303, 3025, 4123
BstKTI	GATC	9 cut(s)	499, 1190, 1836, 2004, 3111, 3450, 3789, 4456, 4471
BstMBI	GATC	9 cut(s)	496, 1187, 1833, 2001, 3108, 3447, 3786, 4453, 4468
BstMWI	GCNNNNNNNGC	6 cut(s)	926, 1001, 1343, 3241, 3580, 3919
BstNI	CCWGG	4 cut(s)	24, 216, 2140, 2544
BstNSI	RCATGY	2 cut(s)	761, 2609
BstSCI	CCNGG	4 cut(s)	22, 214, 2138, 2542
BstSFI	CTRYAG	5 cut(s)	688, 2580, 2634, 3756, 4095
BstSLI	GKGCMC	1 cut(s)	3720
BstUI	CGCG	3 cut(s)	450, 3025, 4437
BstV1I	GCAGC	6 cut(s)	526, 773, 1066, 2310, 2545, 2778
BstV2I	GAAGAC	7 cut(s)	258, 2968, 3105, 3336, 3444, 3675, 3783
BsuRI	GGCC	3 cut(s)	76, 2227, 4219
BtgI	CCRYGG	1 cut(s)	4191
BtgZI	GCGATG	2 cut(s)	1238, 4452
BtrI	CACGTC	2 cut(s)	1400, 1471
BtsI	GCAGTG	4 cut(s)	322, 924, 2964, 4304
BtuMI	TCGCGA	1 cut(s)	4437
BveI	ACCTGC	3 cut(s)	1786, 2156, 2193
Cac8I	GCNNGC	7 cut(s)	544, 709, 759, 922, 1027, 2079, 2190
CaiI	CAGNNNCTG	3 cut(s)	470, 559, 2794
CfoI	GCGC	3 cut(s)	2303, 3025, 4123
Cfr13I	GGNCC	5 cut(s)	212, 857, 1350, 1928, 2096
Csp6I	GTAC	8 cut(s)	666, 939, 1105, 1670, 1967, 2988, 3000, 4402
CspCI	CAANNNNNGTGG	2 cut(s)	98, 133
CviQI	GTAC	8 cut(s)	666, 939, 1105, 1670, 1967, 2988, 3000, 4402
DpnI	GATC	9 cut(s)	498, 1189, 1835, 2003, 3110, 3449, 3788, 4455, 4470
DpnII	GATC	9 cut(s)	496, 1187, 1833, 2001, 3108, 3447, 3786, 4453, 4468
DraI	TTTAAA	2 cut(s)	483, 1716
DriI	GACNNNNNGTC	4 cut(s)	1901, 3409, 3748, 4087
EaeI	YGGCCR	1 cut(s)	2225
Eam1104I	CTCTTC	1 cut(s)	992
Eam1105I	GACNNNNNGTC	4 cut(s)	1901, 3409, 3748, 4087
EarI	CTCTTC	1 cut(s)	992
EciI	GGCGGA	1 cut(s)	1211
Ecl136II	GAGCTC	4 cut(s)	746, 1457, 2680, 4293
Eco130I	CCWWGG	4 cut(s)	127, 1311, 4191, 4310
Eco147I	AGGCCT	1 cut(s)	76
Eco24I	GRGCYC	4 cut(s)	748, 1459, 2682, 4295
Eco31I	GGTCTC	2 cut(s)	639, 4188
Eco32I	GATATC	1 cut(s)	367
Eco47I	GGWCC	5 cut(s)	212, 857, 1350, 1928, 2096
Eco53kI	GAGCTC	4 cut(s)	746, 1457, 2680, 4293
Eco57I	CTGAAG	1 cut(s)	4155
EcoICRI	GAGCTC	4 cut(s)	746, 1457, 2680, 4293
EcoRI	GAATTC	4 cut(s)	1131, 1701, 4271, 4514
EcoRII	CCWGG	4 cut(s)	22, 214, 2138, 2542
EcoRV	GATATC	1 cut(s)	367
EcoT14I	CCWWGG	4 cut(s)	127, 1311, 4191, 4310
EcoT38I	GRGCYC	4 cut(s)	748, 1459, 2682, 4295
ErhI	CCWWGG	4 cut(s)	127, 1311, 4191, 4310
Esp3I	CGTCTC	1 cut(s)	331
FalI	AAGNNNNNCTT	2 cut(s)	2092, 2124
FaqI	GGGAC	4 cut(s)	1941, 2109, 2160, 3133
FauI	CCCGC	3 cut(s)	165, 1650, 4488
FauNDI	CATATG	2 cut(s)	378, 935
FbaI	TGATCA	2 cut(s)	1833, 2001
FblI	GTMKAC	1 cut(s)	723
Fnu4HI	GCNGC	7 cut(s)	540, 787, 1055, 2299, 2559, 2792, 4322
FriOI	GRGCYC	4 cut(s)	748, 1459, 2682, 4295
Fsp4HI	GCNGC	7 cut(s)	540, 787, 1055, 2299, 2559, 2792, 4322
GlaI	GCGC	3 cut(s)	2302, 3024, 4122
GluI	GCNGC	7 cut(s)	540, 787, 1055, 2299, 2559, 2792, 4322
GsaI	CCCAGC	1 cut(s)	2927
GsuI	CTGGAG	3 cut(s)	375, 732, 2550
HaeII	RGCGCY	2 cut(s)	2304, 4124
HaeIII	GGCC	3 cut(s)	76, 2227, 4219
HapII	CCGG	2 cut(s)	2982, 4199
HhaI	GCGC	3 cut(s)	2303, 3025, 4123
Hin1I	GRCGYC	1 cut(s)	695
Hin6I	GCGC	3 cut(s)	2301, 3023, 4121
HinP1I	GCGC	3 cut(s)	2301, 3023, 4121
HindIII	AAGCTT	1 cut(s)	485
HpaII	CCGG	2 cut(s)	2982, 4199
Hpy166II	GTNNAC	8 cut(s)	192, 629, 724, 1033, 1107, 1264, 2603, 4378
Hpy8I	GTNNAC	8 cut(s)	192, 629, 724, 1033, 1107, 1264, 2603, 4378
HpyCH4IV	ACGT	6 cut(s)	169, 695, 822, 1399, 1470, 1633
HpyF10VI	GCNNNNNNNGC	6 cut(s)	926, 1001, 1343, 3241, 3580, 3919
HpySE526I	ACGT	6 cut(s)	169, 695, 822, 1399, 1470, 1633
Hsp92I	GRCGYC	1 cut(s)	695
HspAI	GCGC	3 cut(s)	2301, 3023, 4121
KpnI	GGTACC	1 cut(s)	1673
Ksp22I	TGATCA	2 cut(s)	1833, 2001
Kzo9I	GATC	9 cut(s)	496, 1187, 1833, 2001, 3108, 3447, 3786, 4453, 4468
LmnI	GCTCC	5 cut(s)	751, 1798, 1870, 2038, 4369
Lsp1109I	GCAGC	6 cut(s)	526, 773, 1066, 2310, 2545, 2778
LweI	GCATC	8 cut(s)	1012, 1744, 1773, 2152, 3055, 4156, 4397, 4482
MaeII	ACGT	6 cut(s)	169, 695, 822, 1399, 1470, 1633
MaeIII	GTNAC	8 cut(s)	1012, 1203, 2180, 2425, 2705, 3047, 3494, 3833
MalI	GATC	9 cut(s)	498, 1189, 1835, 2003, 3110, 3449, 3788, 4455, 4470
MbiI	CCGCTC	1 cut(s)	176
MboI	GATC	9 cut(s)	496, 1187, 1833, 2001, 3108, 3447, 3786, 4453, 4468
MfeI	CAATTG	2 cut(s)	657, 2378
MhlI	GDGCHC	6 cut(s)	748, 1459, 1653, 2682, 3720, 4295
MlyI	GAGTC	6 cut(s)	382, 869, 955, 3419, 3758, 4097
MmeI	TCCRAC	2 cut(s)	3070, 4412
MroXI	GAANNNNTTC	2 cut(s)	1131, 2325
MslI	CAYNNNNRTG	4 cut(s)	1286, 1740, 1769, 2598
MspA1I	CMGCKG	2 cut(s)	2712, 2791
MspI	CCGG	2 cut(s)	2982, 4199
MspR9I	CCNGG	4 cut(s)	24, 216, 2140, 2544
MunI	CAATTG	2 cut(s)	657, 2378
Mva1269I	GAATGC	6 cut(s)	2373, 2717, 3025, 3349, 3688, 4027
MvaI	CCWGG	4 cut(s)	24, 216, 2140, 2544
MvnI	CGCG	3 cut(s)	450, 3025, 4437
MwoI	GCNNNNNNNGC	6 cut(s)	926, 1001, 1343, 3241, 3580, 3919
NcoI	CCATGG	1 cut(s)	4191
NdeI	CATATG	2 cut(s)	378, 935
NdeII	GATC	9 cut(s)	496, 1187, 1833, 2001, 3108, 3447, 3786, 4453, 4468
NlaIV	GGNNCC	9 cut(s)	1446, 1671, 1929, 2009, 2097, 3184, 3523, 3862, 4197
NmeAIII	GCCGAG	3 cut(s)	1352, 2203, 4195
NmuCI	GTSAC	4 cut(s)	2705, 3047, 3494, 3833
NruI	TCGCGA	1 cut(s)	4437
NspI	RCATGY	2 cut(s)	761, 2609
OliI	CACNNNNGTG	1 cut(s)	1286
PaeI	GCATGC	1 cut(s)	761
PaqCI	CACCTGC	1 cut(s)	2156
PceI	AGGCCT	1 cut(s)	76
PciI	ACATGT	1 cut(s)	2605
PcsI	WCGNNNNNNNCGW	2 cut(s)	579, 3554
PctI	GAATGC	6 cut(s)	2373, 2717, 3025, 3349, 3688, 4027
PdmI	GAANNNNTTC	2 cut(s)	1131, 2325
PflFI	GACNNNGTC	4 cut(s)	1932, 2100, 3500, 3839
PflMI	CCANNNNNTGG	1 cut(s)	4394
PfoI	TCCNGGA	1 cut(s)	22
PkrI	GCNGC	7 cut(s)	541, 788, 1056, 2300, 2560, 2793, 4323
PleI	GAGTC	6 cut(s)	382, 869, 954, 3418, 3757, 4096
PpsI	GAGTC	6 cut(s)	382, 869, 954, 3418, 3757, 4096
PscI	ACATGT	1 cut(s)	2605
PsiI	TTATAA	1 cut(s)	150
Psp124BI	GAGCTC	4 cut(s)	748, 1459, 2682, 4295
Psp1406I	AACGTT	3 cut(s)	169, 822, 1633
Psp6I	CCWGG	4 cut(s)	22, 214, 2138, 2542
PspFI	CCCAGC	1 cut(s)	2923
PspGI	CCWGG	4 cut(s)	22, 214, 2138, 2542
PspN4I	GGNNCC	9 cut(s)	1446, 1671, 1929, 2009, 2097, 3184, 3523, 3862, 4197
PspPI	GGNCC	5 cut(s)	212, 857, 1350, 1928, 2096
PstI	CTGCAG	2 cut(s)	692, 2638
PstNI	CAGNNNCTG	3 cut(s)	470, 559, 2794
PsyI	GACNNNGTC	4 cut(s)	1932, 2100, 3500, 3839
PvuII	CAGCTG	1 cut(s)	2791
RruI	TCGCGA	1 cut(s)	4437
RsaI	GTAC	8 cut(s)	667, 940, 1106, 1671, 1968, 2989, 3001, 4403
RsaNI	GTAC	8 cut(s)	666, 939, 1105, 1670, 1967, 2988, 3000, 4402
RseI	CAYNNNNRTG	4 cut(s)	1286, 1740, 1769, 2598
SacI	GAGCTC	4 cut(s)	748, 1459, 2682, 4295
SatI	GCNGC	7 cut(s)	540, 787, 1055, 2299, 2559, 2792, 4322
Sau3AI	GATC	9 cut(s)	496, 1187, 1833, 2001, 3108, 3447, 3786, 4453, 4468
Sau96I	GGNCC	5 cut(s)	212, 857, 1350, 1928, 2096
SchI	GAGTC	6 cut(s)	382, 869, 955, 3419, 3758, 4097
ScrFI	CCNGG	4 cut(s)	24, 216, 2140, 2544
SduI	GDGCHC	6 cut(s)	748, 1459, 1653, 2682, 3720, 4295
SfaNI	GCATC	8 cut(s)	1012, 1744, 1773, 2152, 3055, 4156, 4397, 4482
SfcI	CTRYAG	5 cut(s)	688, 2580, 2634, 3756, 4095
SinI	GGWCC	5 cut(s)	212, 857, 1350, 1928, 2096
SmiMI	CAYNNNNRTG	4 cut(s)	1286, 1740, 1769, 2598
SmlI	CTYRAG	7 cut(s)	425, 943, 1217, 3069, 3236, 3575, 3914
SmoI	CTYRAG	7 cut(s)	425, 943, 1217, 3069, 3236, 3575, 3914
SphI	GCATGC	1 cut(s)	761
SseBI	AGGCCT	1 cut(s)	76
SspI	AATATT	1 cut(s)	2879
SstI	GAGCTC	4 cut(s)	748, 1459, 2682, 4295
StuI	AGGCCT	1 cut(s)	76
StyD4I	CCNGG	4 cut(s)	22, 214, 2138, 2542
StyI	CCWWGG	4 cut(s)	127, 1311, 4191, 4310
TaiI	ACGT	6 cut(s)	172, 698, 825, 1402, 1473, 1636
TaqI	TCGA	8 cut(s)	871, 976, 1186, 1453, 1936, 3267, 3606, 4456
TaqII	GACCGA	1 cut(s)	200
TatI	WGTACW	3 cut(s)	665, 1104, 2987
TauI	GCSGC	1 cut(s)	4324
TseFI	GTSAC	4 cut(s)	2705, 3047, 3494, 3833
TseI	GCWGC	6 cut(s)	539, 786, 1054, 2298, 2558, 2791
Tsp45I	GTSAC	4 cut(s)	2705, 3047, 3494, 3833
TspGWI	ACGGA	5 cut(s)	662, 2472, 2711, 3137, 3422
Tth111I	GACNNNGTC	4 cut(s)	1932, 2100, 3500, 3839
Van91I	CCANNNNNTGG	1 cut(s)	4394
VpaK11BI	GGWCC	5 cut(s)	212, 857, 1350, 1928, 2096
XceI	RCATGY	2 cut(s)	761, 2609
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2680
XmaJI	CCTAGG	2 cut(s)	127, 4310
XmiI	GTMKAC	1 cut(s)	723
XmnI	GAANNNNTTC	2 cut(s)	1131, 2325
ZraI	GACGTC	1 cut(s)	696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.