Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010987

Physical Location & Seq

Genomic Coordinates Forward (+)

4790246 .. 4794556

Gene Structure 4311 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1070100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGTGGCGGCAAATCGGAGACAGAGAAGCGGGCAAGCTCCGCCCAAATTCGTTCTCGAATCGAATCAAGTCCACCCGGATCCAAACGCTCCAACTCTCCAATCACAAAGACTTCATCTCCCCTCATCGCGGCTCCGTCAACACTCTTCAGCTGGATTTGACGGAGGCCCGGTACCTGTTATCCGGCGCCTCCGATGCTTCCGCTGCCGTGTTCGATGTCCAGCGCGGGACCGATTACGGAGGCGGCGGAGCTATCACGAGGCACAAGTGCTTGTTTGTGGTGGATAAGCAGCACGAGCACGGGCATAAGTATGCGGTGTCCTCCGCCGTGTGGTATCCGGTCGACACCGGACTGTTTGTGACGGGTTCGTACGATCATCACATCAATGTTTGGGATACGAATACGACGCAGGTAGTGATGGACTTCAAGATGCCGGGGAAGGTGTACCGGACTGCAATGTCCCCGCTGGCAACTTCTCACATGCTCATTGCTGCCGGTACCGAAGATGTTCAAGTTCGCCTCTGTGATATTGCTTCTGGGGCTTTTGCTCACACCTTGTCTGGCCACCGCGATGGTGTGATGACTGTGGAGTGGTCTACTTCTAGCGAGTGGGTATTGCTTACAGGTGGATGTGATGGTGCAATACGTTTTTGGGACATTAGACGTGCCGGATGTTTTCGTGTTTTGGATCAATCTCAGTCTCAGCTTGGGAGGCGTCCACCCATCCTGGAACGCTCTGCCACAATTAAGGGTTCAACAGTTAAGCCCTCGTCATCAAGTCAAAGCACATCTGCAAAAGCTCGGGCACCACAAAGGAAACTTTCTAATGGGAGTGGTGCAAAGCACTCACCCATTGGTAAAAGTCCAGCAAAGGGATCTATGAAGCAAAGATTACATCCAGGGATGCTGTCTAGTCAGGATCGTGCCACTGCCCATTATGGTGCTGTTACTGGATTAAAAGTCACTGAAGACGGCATGTACCTCTTGAGTGCAGGTTCCGATTCAAGATTGAGGTTGTGGGATGTAGAATCTGGCTGCAATACTTTGGTGAACTTTGAAACTGTGCGTATGCAAACCAGCAGGCCCATACAGTTAGCTACAAGTCAGAATTCGGAGCTTGTTTTTGTTCCCTGCATGACAGCTGTGAAGGCATTCAATATGTGGTCGGGTAAAACATCCCTGAAATTTCGCGGTCACTATGAACATGTAAACTGTTGCTGGTTTAGTTCACAGGATCAGGAATTGTACACTGGTGGCAATGATAGACAAATTCTAGTTTGGTCGCCGCCCAGATTGGTTTCTGATGAGGATGAAGGACCCTCTAAGGACGAGGATAATTGGAGCGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0005488 GO:0005575 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006283 GO:0006289 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009893 GO:0009987 GO:0010604 GO:0016567 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031325 GO:0032446 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044877 GO:0045739 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051716 GO:0051865 GO:0060255 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:1901360 GO:1901564 GO:2001020 GO:2001022

KEGG Pathways

Metabolic & Signaling

ko03420 ko04120 map03420 map04120

Pfam Domains

Protein Families

WD40

Protein Analysis

450

Amino Acids

49.52

Weight (kDa)

8.92

Isoelectric Point (pI)

42.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	27 - 140	3.7e-06	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	33 - 150	3.8e-07	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	42 - 180	1.5e-11	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	100 - 235	1.2e-16	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	100 - 223	1.4e-11	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	106 - 223	1.3e-07	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	115 - 222	3.8e-08	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	117 - 219	6.2e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	120 - 223	8.5e-06	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	121 - 229	2.4e-07	WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st	PF24817	158 - 230	3.3e-10	WDHD1 first WD40 domain
WD40_Prp19	PF24814	159 - 232	1.7e-07	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	159 - 229	2e-07	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	162 - 234	2.8e-10	THOC3 beta-propeller domain
WD40	PF00400	183 - 219	1.2e-07	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	307 - 428	2.7e-11	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	307 - 427	3.6e-08	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	308 - 428	6.7e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	310 - 428	3.6e-12	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	365 - 427	3e-07	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010987)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19750 AT1G19750 AT1G27840 AT1G27840 AT1G27840
fragaria_vesca	FvH4_2g33100
malus_domestica	MD15G1070100.v1.1
prunus_persica	Prupe.1G422900_v2.0.a1
pyrus_communis	pycom15g06610
rosa_chinensis	RchiOBHm_Chr6g0309581
rosa_laevigata	RLG00000010551
rosa_multiflora	Rmu_sc0024715.1_g000001 Rmu_sc0030536.1_g000002
rosa_roxburghii	Rroxscaffold_7G00159300
rosa_rugosa	Rorug06G0372900
rosa_samantha	Rh6AG486500 Rh6BG495900 Rh6CG500800 Rh6DG486700
rosa_wichuraiana	Rw6G042370

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	457
Acc36I	ACCTGC	2 cut(s)	400, 983
Acc65I	GGTACC	2 cut(s)	171, 497
AccB1I	GGYRCC	4 cut(s)	171, 185, 497, 805
AccI	GTMKAC	2 cut(s)	342, 596
AccII	CGCG	4 cut(s)	129, 225, 570, 1191
AclWI	GGATC	6 cut(s)	73, 86, 696, 883, 927, 1242
AcoI	YGGCCR	1 cut(s)	562
AcsI	RAATTY	4 cut(s)	46, 1108, 1184, 1269
AcuI	CTGAAG	2 cut(s)	131, 987
AcyI	GRCGYC	2 cut(s)	186, 715
AfaI	GTAC	6 cut(s)	173, 371, 446, 499, 980, 1247
AfiI	CCNNNNNNNGG	3 cut(s)	128, 330, 872
AflIII	ACRYGT	1 cut(s)	1204
AgsI	TTSAA	6 cut(s)	427, 512, 756, 1005, 1058, 1156
AjiI	CACGTC	1 cut(s)	665
AjnI	CCWGG	2 cut(s)	726, 898
AluBI	AGCT	8 cut(s)	37, 151, 251, 706, 800, 1097, 1117, 1142
AluI	AGCT	8 cut(s)	37, 151, 251, 706, 800, 1097, 1117, 1142
Alw21I	GWGCWC	1 cut(s)	300
Alw26I	GTCTC	2 cut(s)	12, 705
AlwI	GGATC	6 cut(s)	73, 86, 696, 883, 927, 1242
Ama87I	CYCGRG	1 cut(s)	801
AoxI	GGCC	3 cut(s)	165, 562, 1082
ApeKI	GCWGC	4 cut(s)	203, 289, 491, 1035
ApoI	RAATTY	4 cut(s)	46, 1108, 1184, 1269
Asp700I	GAANNNNTTC	2 cut(s)	507, 1151
Asp718I	GGTACC	2 cut(s)	171, 497
AspLEI	GCGC	2 cut(s)	188, 225
AspS9I	GGNCC	4 cut(s)	166, 228, 1083, 1316
AsuC2I	CCSGG	3 cut(s)	76, 169, 435
AsuHPI	GGTGA	2 cut(s)	840, 1060
AvaI	CYCGRG	1 cut(s)	801
AvaII	GGWCC	2 cut(s)	228, 1316
BaeGI	GKGCMC	1 cut(s)	808
BalI	TGGCCA	1 cut(s)	564
BamHI	GGATCC	1 cut(s)	78
BanI	GGYRCC	4 cut(s)	171, 185, 497, 805
BauI	CACGAG	2 cut(s)	256, 293
BbsI	GAAGAC	1 cut(s)	975
Bbv12I	GWGCWC	1 cut(s)	300
BbvI	GCAGC	4 cut(s)	190, 301, 478, 1022
BccI	CCATC	4 cut(s)	412, 566, 629, 731
BceAI	ACGGC	3 cut(s)	191, 311, 988
BciT130I	CCWGG	2 cut(s)	728, 900
BciVI	GTATCC	2 cut(s)	345, 388
BcnI	CCSGG	3 cut(s)	76, 169, 435
BcoDI	GTCTC	2 cut(s)	12, 705
BfaI	CTAG	4 cut(s)	603, 912, 1274, 1348
BfoI	RGCGCY	1 cut(s)	189
BfuAI	ACCTGC	2 cut(s)	400, 983
BfuI	GTATCC	2 cut(s)	345, 388
BisI	GCNGC	8 cut(s)	8, 130, 204, 244, 290, 492, 1036, 1286
BlsI	GCNGC	8 cut(s)	9, 131, 205, 245, 291, 493, 1037, 1287
Bme1390I	CCNGG	5 cut(s)	76, 169, 435, 728, 900
Bme18I	GGWCC	2 cut(s)	228, 1316
BmeT110I	CYCGRG	1 cut(s)	801
BmgBI	CACGTC	1 cut(s)	665
BmgT120I	GGNCC	4 cut(s)	166, 228, 1083, 1316
BmiI	GGNNCC	9 cut(s)	80, 133, 173, 187, 229, 499, 807, 997, 1318
BmrFI	CCNGG	5 cut(s)	76, 169, 435, 728, 900
BmsI	GCATC	3 cut(s)	184, 420, 894
BpiI	GAAGAC	1 cut(s)	975
BpuEI	CTTGAG	1 cut(s)	1006
BpuMI	CCSGG	3 cut(s)	76, 169, 435
BsaHI	GRCGYC	2 cut(s)	186, 715
BsaJI	CCNNGG	2 cut(s)	434, 899
BsaWI	WCCGGW	3 cut(s)	337, 347, 447
BsaXI	ACNNNNNCTCC	4 cut(s)	101, 131, 155, 185
Bsc4I	CCNNNNNNNGG	3 cut(s)	128, 330, 872
Bse118I	RCCGGY	1 cut(s)	494
Bse1I	ACTGG	2 cut(s)	955, 1255
Bse3DI	GCAATG	3 cut(s)	462, 486, 1264
BseBI	CCWGG	2 cut(s)	728, 900
BseDI	CCNNGG	2 cut(s)	434, 899
BseGI	GGATG	8 cut(s)	635, 677, 723, 895, 909, 1027, 1175, 1315
BseLI	CCNNNNNNNGG	3 cut(s)	128, 330, 872
BseMI	GCAATG	3 cut(s)	462, 486, 1264
BseMII	CTCAG	2 cut(s)	710, 716
BseNI	ACTGG	2 cut(s)	955, 1255
BseSI	GKGCMC	1 cut(s)	808
BseXI	GCAGC	4 cut(s)	190, 301, 478, 1022
BsgI	GTGCAG	1 cut(s)	1011
Bsh1236I	CGCG	4 cut(s)	129, 225, 570, 1191
Bsh1285I	CGRYCG	1 cut(s)	342
BshFI	GGCC	3 cut(s)	167, 564, 1084
BshNI	GGYRCC	4 cut(s)	171, 185, 497, 805
BsiEI	CGRYCG	1 cut(s)	342
BsiHKAI	GWGCWC	1 cut(s)	300
BsiHKCI	CYCGRG	1 cut(s)	801
BsiSI	CCGG	9 cut(s)	76, 169, 183, 338, 348, 434, 448, 495, 669
BsiWI	CGTACG	1 cut(s)	369
BslFI	GGGAC	3 cut(s)	241, 445, 668
BslI	CCNNNNNNNGG	3 cut(s)	128, 330, 872
BsmAI	GTCTC	2 cut(s)	12, 705
BsmFI	GGGAC	3 cut(s)	241, 445, 668
BsmI	GAATGC	1 cut(s)	1151
BsnI	GGCC	3 cut(s)	167, 564, 1084
BsoBI	CYCGRG	1 cut(s)	801
Bsp1286I	GDGCHC	2 cut(s)	300, 808
Bsp1407I	TGTACA	1 cut(s)	1245
Bsp143I	GATC	6 cut(s)	78, 373, 688, 875, 919, 1234
BspANI	GGCC	3 cut(s)	167, 564, 1084
BspCNI	CTCAG	2 cut(s)	709, 715
BspFNI	CGCG	4 cut(s)	129, 225, 570, 1191
BspLI	GGNNCC	9 cut(s)	80, 133, 173, 187, 229, 499, 807, 997, 1318
BspMI	ACCTGC	2 cut(s)	400, 983
BspPI	GGATC	6 cut(s)	73, 86, 696, 883, 927, 1242
BspT107I	GGYRCC	4 cut(s)	171, 185, 497, 805
BsrDI	GCAATG	3 cut(s)	462, 486, 1264
BsrFI	RCCGGY	1 cut(s)	494
BsrGI	TGTACA	1 cut(s)	1245
BsrI	ACTGG	2 cut(s)	955, 1255
BssAI	RCCGGY	1 cut(s)	494
BssECI	CCNNGG	2 cut(s)	434, 899
BssMI	GATC	6 cut(s)	78, 373, 688, 875, 919, 1234
BssNI	GRCGYC	2 cut(s)	186, 715
BssSI	CACGAG	2 cut(s)	256, 293
Bst2BI	CACGAG	2 cut(s)	256, 293
Bst2UI	CCWGG	2 cut(s)	728, 900
Bst4CI	ACNGT	6 cut(s)	354, 586, 760, 1063, 1092, 1214
Bst6I	CTCTTC	1 cut(s)	150
BstACI	GRCGYC	2 cut(s)	186, 715
BstAUI	TGTACA	1 cut(s)	1245
BstC8I	GCNNGC	4 cut(s)	31, 35, 468, 1082
BstDEI	CTNAG	3 cut(s)	696, 702, 1323
BstF5I	GGATG	8 cut(s)	635, 677, 723, 895, 909, 1027, 1175, 1315
BstFNI	CGCG	4 cut(s)	129, 225, 570, 1191
BstH2I	RGCGCY	1 cut(s)	189
BstHHI	GCGC	2 cut(s)	188, 225
BstKTI	GATC	6 cut(s)	81, 376, 691, 878, 922, 1237
BstMAI	GTCTC	2 cut(s)	12, 705
BstMBI	GATC	6 cut(s)	78, 373, 688, 875, 919, 1234
BstMCI	CGRYCG	1 cut(s)	342
BstMWI	GCNNNNNNNGC	7 cut(s)	39, 194, 203, 295, 539, 712, 1148
BstNI	CCWGG	2 cut(s)	728, 900
BstNSI	RCATGY	3 cut(s)	484, 979, 1208
BstSCI	CCNGG	5 cut(s)	74, 167, 433, 726, 898
BstSLI	GKGCMC	1 cut(s)	808
BstUI	CGCG	4 cut(s)	129, 225, 570, 1191
BstV1I	GCAGC	4 cut(s)	190, 301, 478, 1022
BstV2I	GAAGAC	1 cut(s)	975
BstX2I	RGATCY	2 cut(s)	78, 875
BstXI	CCANNNNNNTGG	1 cut(s)	572
BstYI	RGATCY	2 cut(s)	78, 875
BsuI	GTATCC	2 cut(s)	345, 388
BsuRI	GGCC	3 cut(s)	167, 564, 1084
BtgZI	GCGATG	2 cut(s)	110, 585
BtrI	CACGTC	1 cut(s)	665
BtsCI	GGATG	8 cut(s)	635, 677, 723, 895, 909, 1027, 1175, 1315
BtsI	GCAGTG	1 cut(s)	927
BtsIMutI	CAGTG	3 cut(s)	927, 963, 1248
BveI	ACCTGC	2 cut(s)	400, 983
Cac8I	GCNNGC	4 cut(s)	31, 35, 468, 1082
CfoI	GCGC	2 cut(s)	188, 225
Cfr10I	RCCGGY	1 cut(s)	494
Cfr13I	GGNCC	4 cut(s)	166, 228, 1083, 1316
CseI	GACGC	2 cut(s)	415, 704
Csp6I	GTAC	6 cut(s)	172, 370, 445, 498, 979, 1246
CviAII	CATG	4 cut(s)	481, 976, 1135, 1205
CviQI	GTAC	6 cut(s)	172, 370, 445, 498, 979, 1246
DdeI	CTNAG	3 cut(s)	696, 702, 1323
DinI	GGCGCC	1 cut(s)	187
DpnI	GATC	6 cut(s)	80, 375, 690, 877, 921, 1236
DpnII	GATC	6 cut(s)	78, 373, 688, 875, 919, 1234
DrdI	GACNNNNNNGTC	1 cut(s)	457
DseDI	GACNNNNNNGTC	1 cut(s)	457
EaeI	YGGCCR	1 cut(s)	562
Eam1104I	CTCTTC	1 cut(s)	150
EarI	CTCTTC	1 cut(s)	150
EciI	GGCGGA	3 cut(s)	29, 261, 313
Eco47I	GGWCC	2 cut(s)	228, 1316
Eco57I	CTGAAG	2 cut(s)	131, 987
Eco88I	CYCGRG	1 cut(s)	801
EcoO109I	RGGNCCY	1 cut(s)	1316
EcoRI	GAATTC	1 cut(s)	1108
EcoRII	CCWGG	2 cut(s)	726, 898
EgeI	GGCGCC	1 cut(s)	187
EheI	GGCGCC	1 cut(s)	187
FaeI	CATG	4 cut(s)	484, 979, 1138, 1208
FaqI	GGGAC	3 cut(s)	241, 445, 668
FatI	CATG	4 cut(s)	480, 975, 1134, 1204
FauI	CCCGC	3 cut(s)	22, 218, 471
FblI	GTMKAC	2 cut(s)	342, 596
Fnu4HI	GCNGC	8 cut(s)	8, 130, 204, 244, 290, 492, 1036, 1286
FokI	GGATG	8 cut(s)	642, 684, 710, 882, 916, 1034, 1162, 1322
Fsp4HI	GCNGC	8 cut(s)	8, 130, 204, 244, 290, 492, 1036, 1286
FspBI	CTAG	4 cut(s)	603, 912, 1274, 1348
GlaI	GCGC	2 cut(s)	187, 224
GluI	GCNGC	8 cut(s)	8, 130, 204, 244, 290, 492, 1036, 1286
HaeII	RGCGCY	1 cut(s)	189
HaeIII	GGCC	3 cut(s)	167, 564, 1084
HapII	CCGG	9 cut(s)	76, 169, 183, 338, 348, 434, 448, 495, 669
HgaI	GACGC	2 cut(s)	415, 704
HhaI	GCGC	2 cut(s)	188, 225
Hin1I	GRCGYC	2 cut(s)	186, 715
Hin1II	CATG	4 cut(s)	484, 979, 1138, 1208
Hin6I	GCGC	2 cut(s)	186, 223
HinP1I	GCGC	2 cut(s)	186, 223
HincII	GTYRAC	2 cut(s)	139, 343
HindII	GTYRAC	2 cut(s)	139, 343
HinfI	GANTC	4 cut(s)	58, 63, 1001, 1028
HpaII	CCGG	9 cut(s)	76, 169, 183, 338, 348, 434, 448, 495, 669
HphI	GGTGA	2 cut(s)	840, 1060
Hpy188I	TCNGA	6 cut(s)	17, 193, 1000, 1107, 1114, 1303
Hpy188III	TCNNGA	7 cut(s)	55, 256, 427, 917, 985, 1005, 1238
Hpy99I	CGWCG	1 cut(s)	409
HpyAV	CCTTC	3 cut(s)	433, 1141, 1307
HpyCH4III	ACNGT	6 cut(s)	354, 586, 760, 1063, 1092, 1214
HpyCH4IV	ACGT	2 cut(s)	646, 664
HpyCH4V	TGCA	8 cut(s)	455, 641, 794, 839, 992, 1038, 1072, 1134
HpyF10VI	GCNNNNNNNGC	7 cut(s)	39, 194, 203, 295, 539, 712, 1148
HpyF3I	CTNAG	3 cut(s)	696, 702, 1323
HpySE526I	ACGT	2 cut(s)	646, 664
Hsp92I	GRCGYC	2 cut(s)	186, 715
Hsp92II	CATG	4 cut(s)	484, 979, 1138, 1208
HspAI	GCGC	2 cut(s)	186, 223
KasI	GGCGCC	1 cut(s)	185
KpnI	GGTACC	2 cut(s)	175, 501
Kzo9I	GATC	6 cut(s)	78, 373, 688, 875, 919, 1234
LmnI	GCTCC	6 cut(s)	42, 93, 137, 248, 1114, 1341
Lsp1109I	GCAGC	4 cut(s)	190, 301, 478, 1022
LweI	GCATC	3 cut(s)	184, 420, 894
MaeI	CTAG	4 cut(s)	603, 912, 1274, 1348
MaeII	ACGT	2 cut(s)	646, 664
MaeIII	GTNAC	4 cut(s)	358, 946, 961, 1193
MalI	GATC	6 cut(s)	80, 375, 690, 877, 921, 1236
MboI	GATC	6 cut(s)	78, 373, 688, 875, 919, 1234
MboII	GAAGA	3 cut(s)	137, 515, 980
MflI	RGATCY	2 cut(s)	78, 875
MhlI	GDGCHC	2 cut(s)	300, 808
MlsI	TGGCCA	1 cut(s)	564
MluCI	AATT	7 cut(s)	46, 744, 1108, 1184, 1241, 1269, 1336
MluNI	TGGCCA	1 cut(s)	564
Mly113I	GGCGCC	1 cut(s)	186
MmeI	TCCRAC	1 cut(s)	115
Mox20I	TGGCCA	1 cut(s)	564
MroXI	GAANNNNTTC	2 cut(s)	507, 1151
MscI	TGGCCA	1 cut(s)	564
MseI	TTAA	3 cut(s)	747, 762, 956
MslI	CAYNNNNRTG	4 cut(s)	309, 384, 570, 939
Msp20I	TGGCCA	1 cut(s)	564
MspA1I	CMGCKG	4 cut(s)	151, 203, 466, 1142
MspI	CCGG	9 cut(s)	76, 169, 183, 338, 348, 434, 448, 495, 669
MspR9I	CCNGG	5 cut(s)	76, 169, 435, 728, 900
Mva1269I	GAATGC	1 cut(s)	1151
MvaI	CCWGG	2 cut(s)	728, 900
MvnI	CGCG	4 cut(s)	129, 225, 570, 1191
MwoI	GCNNNNNNNGC	7 cut(s)	39, 194, 203, 295, 539, 712, 1148
NarI	GGCGCC	1 cut(s)	186
NciI	CCSGG	3 cut(s)	76, 169, 435
NdeII	GATC	6 cut(s)	78, 373, 688, 875, 919, 1234
NlaIII	CATG	4 cut(s)	484, 979, 1138, 1208
NlaIV	GGNNCC	9 cut(s)	80, 133, 173, 187, 229, 499, 807, 997, 1318
NmuCI	GTSAC	3 cut(s)	358, 961, 1193
NspI	RCATGY	3 cut(s)	484, 979, 1208
PciI	ACATGT	1 cut(s)	1204
PctI	GAATGC	1 cut(s)	1151
PdmI	GAANNNNTTC	2 cut(s)	507, 1151
PfeI	GAWTC	4 cut(s)	58, 63, 1001, 1028
Pfl23II	CGTACG	1 cut(s)	369
PfoI	TCCNGGA	1 cut(s)	726
PkrI	GCNGC	8 cut(s)	9, 131, 205, 245, 291, 493, 1037, 1287
PluTI	GGCGCC	1 cut(s)	189
PpuMI	RGGWCCY	1 cut(s)	1316
PscI	ACATGT	1 cut(s)	1204
Psp5II	RGGWCCY	1 cut(s)	1316
Psp6I	CCWGG	2 cut(s)	726, 898
PspGI	CCWGG	2 cut(s)	726, 898
PspLI	CGTACG	1 cut(s)	369
PspN4I	GGNNCC	9 cut(s)	80, 133, 173, 187, 229, 499, 807, 997, 1318
PspPI	GGNCC	4 cut(s)	166, 228, 1083, 1316
PspPPI	RGGWCCY	1 cut(s)	1316
PsuI	RGATCY	2 cut(s)	78, 875
PvuII	CAGCTG	2 cut(s)	151, 1142
RsaI	GTAC	6 cut(s)	173, 371, 446, 499, 980, 1247
RsaNI	GTAC	6 cut(s)	172, 370, 445, 498, 979, 1246
RseI	CAYNNNNRTG	4 cut(s)	309, 384, 570, 939
SalI	GTCGAC	1 cut(s)	341
SaqAI	TTAA	3 cut(s)	747, 762, 956
SatI	GCNGC	8 cut(s)	8, 130, 204, 244, 290, 492, 1036, 1286
Sau3AI	GATC	6 cut(s)	78, 373, 688, 875, 919, 1234
Sau96I	GGNCC	4 cut(s)	166, 228, 1083, 1316
ScrFI	CCNGG	5 cut(s)	76, 169, 435, 728, 900
SduI	GDGCHC	2 cut(s)	300, 808
SfaNI	GCATC	3 cut(s)	184, 420, 894
SfoI	GGCGCC	1 cut(s)	187
SinI	GGWCC	2 cut(s)	228, 1316
SmiMI	CAYNNNNRTG	4 cut(s)	309, 384, 570, 939
SmlI	CTYRAG	1 cut(s)	985
SmoI	CTYRAG	1 cut(s)	985
Sse9I	AATT	7 cut(s)	46, 744, 1108, 1184, 1241, 1269, 1336
SspDI	GGCGCC	1 cut(s)	185
SspMI	CTAG	4 cut(s)	603, 912, 1274, 1348
StyD4I	CCNGG	5 cut(s)	74, 167, 433, 726, 898
TaaI	ACNGT	6 cut(s)	354, 586, 760, 1063, 1092, 1214
TaiI	ACGT	2 cut(s)	649, 667
TaqI	TCGA	4 cut(s)	56, 61, 213, 342
TaqII	GACCGA	1 cut(s)	245
TasI	AATT	7 cut(s)	46, 744, 1108, 1184, 1241, 1269, 1336
TatI	WGTACW	1 cut(s)	1245
TauI	GCSGC	4 cut(s)	10, 132, 246, 1288
TfiI	GAWTC	4 cut(s)	58, 63, 1001, 1028
Tru1I	TTAA	3 cut(s)	747, 762, 956
Tru9I	TTAA	3 cut(s)	747, 762, 956
TscAI	CASTG	3 cut(s)	934, 970, 1255
TseFI	GTSAC	3 cut(s)	358, 961, 1193
TseI	GCWGC	4 cut(s)	203, 289, 491, 1035
Tsp45I	GTSAC	3 cut(s)	358, 961, 1193
TspDTI	ATGAA	4 cut(s)	103, 896, 1215, 1326
TspGWI	ACGGA	3 cut(s)	124, 176, 252
TspRI	CASTG	3 cut(s)	934, 970, 1255
VpaK11BI	GGWCC	2 cut(s)	228, 1316
XapI	RAATTY	4 cut(s)	46, 1108, 1184, 1269
XceI	RCATGY	3 cut(s)	484, 979, 1208
XmiI	GTMKAC	2 cut(s)	342, 596
XmnI	GAANNNNTTC	2 cut(s)	507, 1151
XspI	CTAG	4 cut(s)	603, 912, 1274, 1348

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.