Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0016060

Physical Location & Seq

Genomic Coordinates Reverse (-)

13898801 .. 13901817

Gene Structure 3017 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1177600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1605 bp

ATGGAACAGGAGCTCTTCTTTTCGTGGCTCAAATTTGTTTCCATATCTTTAATGGTGTTGGTTTTTTCTCTGAGGATGGCGGTGGTTCTCTGGTGGAGGCCCAGAAAAATTGAACAACATTTTGGGAAGCAAGGGATCAGAGGACCCCCTTACCGTTTCTTCATTGGAAATTACAAAGAACTTGCTGGCATGATGATCAAGGCCTCCTCTCAAACCCTTCCTTCCTCCTCTCACCATATTCTCCCTAGAGTTCTTCCTTTTTACCATCACTGGAGAAAAATCTATGGTGCAACATTTCTTGTGTGGTTTGGACCTACAGTTCGACTCACAGTTGCTAATCCTGATCTAATTAGGGAAATATTTACCTCGAAATCAGAATTTTATGAGAAAAACGAGGCTCACCCACTTGTTAAAAAACTGGAAGGTAATGGCCTCCTAAGTCTCAAAGGTGAAAAATGGGCTCGCCACAGAAAAATTATTACCCCTACCTTCCATATGGAGAATGTCAAGTTGTTGATACCGGTGATGGCAACAAGTATAGTAGACGTGATGGACAAATGGTCAGCAATGTCCAGCTCCGGTGAGGTAGAGATTGAAGTTTCCGAGTGGTTCCAAAACCTAACTGAAGACATAATCACCCGTACGGCGTTCGGAAGCAGCTACGAAGATGGAAAGGCCGTTTTCCGGCTACAAGCTCAGCAAATGGCGTTTGTTACCGATGCTTTTCAAAAAGTTTGCATCCCTGGTTACAGATTCCTGCCCACAAGAAGGAACATAAATTCTTGGAAATTGGACAAGCAAATTAGGAAGTCATTGATAAAGCTGATCGATCGACGGGTGGAGAATTCGAACTCGGGCGACAATGTGCATGAAAAATGTCCAAAAGATTTACTAGGTCTTATGATTCAGGCCTCAAATTCTTCCCCTTCCTCCAATATCACTGTCAATGACATTGTTGAAGAATGCAAGAGCTTCTTCTTTGCGGGAAAACACACCACTTCCAATTTGCTGACATGGACAACGGCTCTCCTAGCAATGCACCCACAGTGGCAGCTACAGGCACGCGACGAGGTGCTGAGGGTGTGTGGATCACGTGACATACCCACAAAAGATGATGTTGTAAAGCTTAAGACGCTGAATATGATCCTCAATGAATCACTACGATTGTACCCACCTGTGATCGCGACGATCCGGCGGTCGAGAACAGATGTGGAGCTCGGGGGATACAAGGTCCCACGTGGAACCGAGCTTCTGATTCCGATCATAGCCGTCCATCATGATCAGAGCATATGGGGCAATGATGCGAAGGAGTTCAACCCGGGACGGTTCGCGGAGGGCGTGGCACGGGCCGCCAAGCACCCGGTGGCGTTCATTCCTTTCGGGCTTGGGGTCCGCACATGCATCGGGCAGAATCTAGCGCTGTTGCAGGCAAAACTAGCCCTTGCCATCATGCTTCAACGCTTCTCTTTCAGGCTGGCCCCCACCTATCAACATGCACCTACGGTACTGATGCTACTTCACCCACAATATGGTGTACCCATCATTTTCCAACGCCTGCCACAACATGATCAGGGTCAAGATCAAGATCGAAATCAAGGGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0006629 GO:0008150 GO:0008152 GO:0008202 GO:0008395 GO:0009719 GO:0009725 GO:0009741 GO:0010033 GO:0010268 GO:0010817 GO:0014070 GO:0016128 GO:0016131 GO:0016491 GO:0033993 GO:0042221 GO:0042445 GO:0042592 GO:0044238 GO:0048878 GO:0050896 GO:0055088 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901360 GO:1901615 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00905 map00905

Pfam Domains

Protein Families

p450

Protein Analysis

535

Amino Acids

60.99

Weight (kDa)

9.63

Isoelectric Point (pI)

39.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	88 - 498	1.7e-85	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016060)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26710
fragaria_vesca	FvH4_1g03910
malus_domestica	MD02G1037300.v1.1 MD15G1177600.v1.1
prunus_persica	Prupe.7G237900_v2.0.a1
pyrus_communis	pycom02g03060 pycom15g15890
rosa_chinensis	RchiOBHm_Chr2g0089381
rosa_laevigata	RLG00000016022
rosa_multiflora	Rmu_sc0000197.1_g000024
rosa_roxburghii	Rroxscaffold_2G00152010
rosa_rugosa	Rorug01G0490300
rosa_samantha	Rh2AG045400 Rh2DG045300
rosa_wichuraiana	Rw2G003730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1529
AccI	GTMKAC	1 cut(s)	543
AccII	CGCG	3 cut(s)	1065, 1184, 1331
AciI	CCGC	6 cut(s)	80, 983, 1195, 1331, 1350, 1393
AclWI	GGATC	4 cut(s)	143, 1096, 1138, 1183
AcsI	RAATTY	5 cut(s)	32, 377, 778, 844, 916
AcuI	CTGAAG	1 cut(s)	645
AcvI	CACGTG	2 cut(s)	1094, 1238
AdeI	CACNNNGTG	1 cut(s)	1363
AfaI	GTAC	4 cut(s)	643, 1169, 1506, 1536
AfeI	AGCGCT	1 cut(s)	1419
AfiI	CCNNNNNNNGG	3 cut(s)	684, 768, 1529
AflII	CTTAAG	1 cut(s)	1127
AgeI	ACCGGT	1 cut(s)	520
AgsI	TTSAA	6 cut(s)	113, 596, 728, 959, 1315, 1457
AhdI	GACNNNNNGTC	1 cut(s)	559
AjiI	CACGTC	1 cut(s)	547
AjnI	CCWGG	1 cut(s)	742
AjuI	GAANNNNNNNTTGG	2 cut(s)	105, 137
AloI	GAACNNNNNNTCC	2 cut(s)	303, 335
Alw21I	GWGCWC	2 cut(s)	15, 1218
Alw26I	GTCTC	1 cut(s)	446
AlwI	GGATC	4 cut(s)	143, 1096, 1138, 1183
Ama87I	CYCGRG	3 cut(s)	853, 1217, 1318
Aor51HI	AGCGCT	1 cut(s)	1419
AoxI	GGCC	8 cut(s)	98, 201, 430, 675, 909, 1347, 1476, 1598
ApeKI	GCWGC	2 cut(s)	657, 1051
ApoI	RAATTY	5 cut(s)	32, 377, 778, 844, 916
ArsI	GACNNNNNNTTYG	2 cut(s)	303, 335
AsiGI	ACCGGT	1 cut(s)	520
Asp700I	GAANNNNTTC	1 cut(s)	1310
AspLEI	GCGC	1 cut(s)	1420
AspS9I	GGNCC	8 cut(s)	99, 143, 311, 1231, 1347, 1390, 1477, 1598
AsuC2I	CCSGG	3 cut(s)	1319, 1320, 1361
AsuHPI	GGTGA	7 cut(s)	224, 392, 461, 535, 593, 628, 1511
AsuII	TTCGAA	1 cut(s)	848
AvaI	CYCGRG	3 cut(s)	853, 1217, 1318
AvaII	GGWCC	4 cut(s)	143, 311, 1231, 1390
BaeI	ACNNNNGTAYC	2 cut(s)	1151, 1184
BanII	GRGCYC	3 cut(s)	15, 463, 1218
BbrPI	CACGTG	2 cut(s)	1094, 1238
BbsI	GAAGAC	1 cut(s)	633
Bbv12I	GWGCWC	2 cut(s)	15, 1218
BbvCI	CCTCAGC	1 cut(s)	1076
BbvI	GCAGC	2 cut(s)	669, 1063
BccI	CCATC	8 cut(s)	70, 273, 520, 544, 662, 1281, 1454, 1547
BceAI	ACGGC	4 cut(s)	660, 662, 1038, 1253
BcgI	CGANNNNNNTGC	2 cut(s)	1384, 1418
BciT130I	CCWGG	1 cut(s)	744
BciVI	GTATCC	1 cut(s)	1217
BclI	TGATCA	3 cut(s)	195, 1279, 1567
BcnI	CCSGG	3 cut(s)	1319, 1320, 1361
BcoDI	GTCTC	1 cut(s)	446
BfaI	CTAG	5 cut(s)	246, 893, 1031, 1415, 1436
BfmI	CTRYAG	2 cut(s)	315, 1055
BfoI	RGCGCY	1 cut(s)	1421
BfrI	CTTAAG	1 cut(s)	1127
BfuI	GTATCC	1 cut(s)	1217
BisI	GCNGC	3 cut(s)	658, 1052, 1350
BlpI	GCTNAGC	1 cut(s)	696
BlsI	GCNGC	3 cut(s)	659, 1053, 1351
Bme1390I	CCNGG	4 cut(s)	744, 1319, 1320, 1361
Bme18I	GGWCC	4 cut(s)	143, 311, 1231, 1390
BmeRI	GACNNNNNGTC	1 cut(s)	559
BmeT110I	CYCGRG	3 cut(s)	853, 1217, 1318
BmgBI	CACGTC	1 cut(s)	547
BmgT120I	GGNCC	8 cut(s)	99, 143, 311, 1231, 1347, 1390, 1477, 1598
BmiI	GGNNCC	6 cut(s)	145, 611, 1233, 1243, 1391, 1479
BmrFI	CCNGG	4 cut(s)	744, 1319, 1320, 1361
BmsI	GCATC	5 cut(s)	709, 747, 1291, 1410, 1500
BpiI	GAAGAC	1 cut(s)	633
BpmI	CTGGAG	1 cut(s)	292
Bpu10I	CCTNAGC	1 cut(s)	1076
Bpu1102I	GCTNAGC	1 cut(s)	696
Bpu14I	TTCGAA	1 cut(s)	848
BpuMI	CCSGG	3 cut(s)	1319, 1320, 1361
Bsa29I	ATCGAT	1 cut(s)	828
BsaAI	YACGTR	2 cut(s)	1094, 1238
BsaBI	GATNNNNATC	3 cut(s)	1259, 1584, 1590
BsaJI	CCNNGG	2 cut(s)	742, 1318
BsaWI	WCCGGW	2 cut(s)	520, 578
Bsc4I	CCNNNNNNNGG	3 cut(s)	684, 768, 1529
Bse118I	RCCGGY	1 cut(s)	520
Bse1I	ACTGG	2 cut(s)	275, 423
Bse3DI	GCAATG	3 cut(s)	573, 1041, 1303
Bse8I	GATNNNNATC	3 cut(s)	1259, 1584, 1590
BseBI	CCWGG	1 cut(s)	744
BseCI	ATCGAT	1 cut(s)	828
BseDI	CCNNGG	2 cut(s)	742, 1318
BseGI	GGATG	2 cut(s)	81, 738
BseJI	GATNNNNATC	3 cut(s)	1259, 1584, 1590
BseLI	CCNNNNNNNGG	3 cut(s)	684, 768, 1529
BseMI	GCAATG	3 cut(s)	573, 1041, 1303
BseMII	CTCAG	3 cut(s)	62, 710, 1067
BseNI	ACTGG	2 cut(s)	275, 423
BseRI	GAGGAG	2 cut(s)	196, 217
BseXI	GCAGC	2 cut(s)	669, 1063
Bsh1236I	CGCG	3 cut(s)	1065, 1184, 1331
Bsh1285I	CGRYCG	2 cut(s)	832, 1199
BshFI	GGCC	8 cut(s)	100, 203, 432, 677, 911, 1349, 1478, 1600
BshTI	ACCGGT	1 cut(s)	520
BshVI	ATCGAT	1 cut(s)	828
BsiEI	CGRYCG	2 cut(s)	832, 1199
BsiHKAI	GWGCWC	2 cut(s)	15, 1218
BsiHKCI	CYCGRG	3 cut(s)	853, 1217, 1318
BsiSI	CCGG	6 cut(s)	521, 579, 685, 1192, 1319, 1361
BsiWI	CGTACG	1 cut(s)	641
BslFI	GGGAC	2 cut(s)	1217, 1335
BslI	CCNNNNNNNGG	3 cut(s)	684, 768, 1529
BsmAI	GTCTC	1 cut(s)	446
BsmFI	GGGAC	2 cut(s)	1217, 1335
BsmI	GAATGC	1 cut(s)	968
BsnI	GGCC	8 cut(s)	100, 203, 432, 677, 911, 1349, 1478, 1600
BsoBI	CYCGRG	3 cut(s)	853, 1217, 1318
Bsp119I	TTCGAA	1 cut(s)	848
Bsp1286I	GDGCHC	3 cut(s)	15, 463, 1218
Bsp1720I	GCTNAGC	1 cut(s)	696
Bsp68I	TCGCGA	1 cut(s)	1184
BspACI	CCGC	6 cut(s)	80, 983, 1195, 1331, 1350, 1393
BspANI	GGCC	8 cut(s)	100, 203, 432, 677, 911, 1349, 1478, 1600
BspCNI	CTCAG	3 cut(s)	63, 709, 1068
BspDI	ATCGAT	1 cut(s)	828
BspFNI	CGCG	3 cut(s)	1065, 1184, 1331
BspHI	TCATGA	1 cut(s)	1276
BspLI	GGNNCC	6 cut(s)	145, 611, 1233, 1243, 1391, 1479
BspPI	GGATC	4 cut(s)	143, 1096, 1138, 1183
BspQI	GCTCTTC	1 cut(s)	20
BspT104I	TTCGAA	1 cut(s)	848
BspTI	CTTAAG	1 cut(s)	1127
BsrDI	GCAATG	3 cut(s)	573, 1041, 1303
BsrFI	RCCGGY	1 cut(s)	520
BsrI	ACTGG	2 cut(s)	275, 423
BssAI	RCCGGY	1 cut(s)	520
BssECI	CCNNGG	2 cut(s)	742, 1318
Bst2UI	CCWGG	1 cut(s)	744
Bst4CI	ACNGT	7 cut(s)	155, 319, 331, 943, 1047, 1326, 1504
Bst6I	CTCTTC	1 cut(s)	20
BstAFI	CTTAAG	1 cut(s)	1127
BstBAI	YACGTR	2 cut(s)	1094, 1238
BstBI	TTCGAA	1 cut(s)	848
BstC8I	GCNNGC	6 cut(s)	187, 463, 1063, 1428, 1476, 1556
BstDEI	CTNAG	4 cut(s)	71, 437, 696, 1076
BstF5I	GGATG	2 cut(s)	81, 738
BstFNI	CGCG	3 cut(s)	1065, 1184, 1331
BstH2I	RGCGCY	1 cut(s)	1421
BstHHI	GCGC	1 cut(s)	1420
BstMAI	GTCTC	1 cut(s)	446
BstMCI	CGRYCG	2 cut(s)	832, 1199
BstMWI	GCNNNNNNNGC	5 cut(s)	1031, 1132, 1293, 1349, 1436
BstNI	CCWGG	1 cut(s)	744
BstNSI	RCATGY	2 cut(s)	1401, 1496
BstSCI	CCNGG	4 cut(s)	742, 1317, 1318, 1359
BstSFI	CTRYAG	2 cut(s)	315, 1055
BstUI	CGCG	3 cut(s)	1065, 1184, 1331
BstV1I	GCAGC	2 cut(s)	669, 1063
BstV2I	GAAGAC	1 cut(s)	633
Bsu15I	ATCGAT	1 cut(s)	828
BsuI	GTATCC	1 cut(s)	1217
BsuRI	GGCC	8 cut(s)	100, 203, 432, 677, 911, 1349, 1478, 1600
BsuTUI	ATCGAT	1 cut(s)	828
BtrI	CACGTC	1 cut(s)	547
BtsCI	GGATG	2 cut(s)	81, 738
BtsIMutI	CAGTG	3 cut(s)	268, 939, 1052
BtuMI	TCGCGA	1 cut(s)	1184
Cac8I	GCNNGC	6 cut(s)	187, 463, 1063, 1428, 1476, 1556
CciI	TCATGA	1 cut(s)	1276
CfoI	GCGC	1 cut(s)	1420
Cfr10I	RCCGGY	1 cut(s)	520
Cfr13I	GGNCC	8 cut(s)	99, 143, 311, 1231, 1347, 1390, 1477, 1598
Cfr9I	CCCGGG	1 cut(s)	1318
ClaI	ATCGAT	1 cut(s)	828
CseI	GACGC	1 cut(s)	1141
Csp6I	GTAC	4 cut(s)	642, 1168, 1505, 1535
CspAI	ACCGGT	1 cut(s)	520
CviAII	CATG	9 cut(s)	190, 869, 1014, 1277, 1398, 1450, 1493, 1565, 1602
CviQI	GTAC	4 cut(s)	642, 1168, 1505, 1535
DdeI	CTNAG	4 cut(s)	71, 437, 696, 1076
DraIII	CACNNNGTG	1 cut(s)	1363
DriI	GACNNNNNGTC	1 cut(s)	559
Eam1104I	CTCTTC	1 cut(s)	20
Eam1105I	GACNNNNNGTC	1 cut(s)	559
EarI	CTCTTC	1 cut(s)	20
Ecl136II	GAGCTC	2 cut(s)	13, 1216
Eco147I	AGGCCT	2 cut(s)	203, 911
Eco24I	GRGCYC	3 cut(s)	15, 463, 1218
Eco47I	GGWCC	4 cut(s)	143, 311, 1231, 1390
Eco47III	AGCGCT	1 cut(s)	1419
Eco53kI	GAGCTC	2 cut(s)	13, 1216
Eco57I	CTGAAG	1 cut(s)	645
Eco72I	CACGTG	2 cut(s)	1094, 1238
Eco88I	CYCGRG	3 cut(s)	853, 1217, 1318
EcoICRI	GAGCTC	2 cut(s)	13, 1216
EcoO109I	RGGNCCY	2 cut(s)	143, 1231
EcoRI	GAATTC	1 cut(s)	844
EcoRII	CCWGG	1 cut(s)	742
EcoT22I	ATGCAT	1 cut(s)	1403
EcoT38I	GRGCYC	3 cut(s)	15, 463, 1218
FaeI	CATG	9 cut(s)	193, 872, 1017, 1280, 1401, 1453, 1496, 1568, 1605
FalI	AAGNNNNNCTT	2 cut(s)	959, 991
FaqI	GGGAC	2 cut(s)	1217, 1335
FatI	CATG	9 cut(s)	189, 868, 1013, 1276, 1397, 1449, 1492, 1564, 1601
FauI	CCCGC	1 cut(s)	976
FauNDI	CATATG	2 cut(s)	495, 1289
FbaI	TGATCA	3 cut(s)	195, 1279, 1567
FblI	GTMKAC	1 cut(s)	543
Fnu4HI	GCNGC	3 cut(s)	658, 1052, 1350
FokI	GGATG	2 cut(s)	88, 725
FriOI	GRGCYC	3 cut(s)	15, 463, 1218
Fsp4HI	GCNGC	3 cut(s)	658, 1052, 1350
FspBI	CTAG	5 cut(s)	246, 893, 1031, 1415, 1436
GlaI	GCGC	1 cut(s)	1419
GluI	GCNGC	3 cut(s)	658, 1052, 1350
GsuI	CTGGAG	1 cut(s)	292
HaeII	RGCGCY	1 cut(s)	1421
HaeIII	GGCC	8 cut(s)	100, 203, 432, 677, 911, 1349, 1478, 1600
HapII	CCGG	6 cut(s)	521, 579, 685, 1192, 1319, 1361
HgaI	GACGC	1 cut(s)	1141
HhaI	GCGC	1 cut(s)	1420
Hin1II	CATG	9 cut(s)	193, 872, 1017, 1280, 1401, 1453, 1496, 1568, 1605
Hin6I	GCGC	1 cut(s)	1418
HinP1I	GCGC	1 cut(s)	1418
HindIII	AAGCTT	1 cut(s)	1124
HinfI	GANTC	6 cut(s)	324, 753, 904, 1154, 1255, 1411
HpaII	CCGG	6 cut(s)	521, 579, 685, 1192, 1319, 1361
HphI	GGTGA	7 cut(s)	224, 392, 461, 535, 593, 628, 1511
Hpy166II	GTNNAC	2 cut(s)	544, 1535
Hpy188I	TCNGA	8 cut(s)	72, 140, 376, 604, 653, 1254, 1260, 1284
Hpy188III	TCNNGA	6 cut(s)	341, 1183, 1200, 1277, 1577, 1583
Hpy8I	GTNNAC	2 cut(s)	544, 1535
Hpy99I	CGWCG	3 cut(s)	837, 1070, 1189
HpyAV	CCTTC	7 cut(s)	227, 231, 416, 499, 762, 936, 1300
HpyCH4III	ACNGT	7 cut(s)	155, 319, 331, 943, 1047, 1326, 1504
HpyCH4IV	ACGT	3 cut(s)	546, 1093, 1237
HpyCH4V	TGCA	8 cut(s)	290, 738, 868, 966, 1039, 1401, 1426, 1496
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1031, 1132, 1293, 1349, 1436
HpyF3I	CTNAG	4 cut(s)	71, 437, 696, 1076
HpySE526I	ACGT	3 cut(s)	546, 1093, 1237
Hsp92II	CATG	9 cut(s)	193, 872, 1017, 1280, 1401, 1453, 1496, 1568, 1605
HspAI	GCGC	1 cut(s)	1418
Ksp22I	TGATCA	3 cut(s)	195, 1279, 1567
LguI	GCTCTTC	1 cut(s)	20
LmnI	GCTCC	3 cut(s)	10, 581, 1213
Lsp1109I	GCAGC	2 cut(s)	669, 1063
LweI	GCATC	5 cut(s)	709, 747, 1291, 1410, 1500
MaeI	CTAG	5 cut(s)	246, 893, 1031, 1415, 1436
MaeII	ACGT	3 cut(s)	546, 1093, 1237
MaeIII	GTNAC	3 cut(s)	712, 746, 1094
MboII	GAAGA	8 cut(s)	7, 151, 245, 638, 677, 912, 967, 971
MhlI	GDGCHC	3 cut(s)	15, 463, 1218
MlyI	GAGTC	1 cut(s)	318
MmeI	TCCRAC	1 cut(s)	1573
Mph1103I	ATGCAT	1 cut(s)	1403
MroXI	GAANNNNTTC	1 cut(s)	1310
MseI	TTAA	3 cut(s)	50, 411, 1128
MspCI	CTTAAG	1 cut(s)	1127
MspI	CCGG	6 cut(s)	521, 579, 685, 1192, 1319, 1361
MspR9I	CCNGG	4 cut(s)	744, 1319, 1320, 1361
Mva1269I	GAATGC	1 cut(s)	968
MvaI	CCWGG	1 cut(s)	744
MvnI	CGCG	3 cut(s)	1065, 1184, 1331
MwoI	GCNNNNNNNGC	5 cut(s)	1031, 1132, 1293, 1349, 1436
NciI	CCSGG	3 cut(s)	1319, 1320, 1361
NdeI	CATATG	2 cut(s)	495, 1289
NlaIII	CATG	9 cut(s)	193, 872, 1017, 1280, 1401, 1453, 1496, 1568, 1605
NlaIV	GGNNCC	6 cut(s)	145, 611, 1233, 1243, 1391, 1479
NmuCI	GTSAC	1 cut(s)	1094
NruI	TCGCGA	1 cut(s)	1184
NsiI	ATGCAT	1 cut(s)	1403
NspI	RCATGY	2 cut(s)	1401, 1496
NspV	TTCGAA	1 cut(s)	848
PagI	TCATGA	1 cut(s)	1276
PceI	AGGCCT	2 cut(s)	203, 911
PciSI	GCTCTTC	1 cut(s)	20
PcsI	WCGNNNNNNNCGW	1 cut(s)	1335
PctI	GAATGC	1 cut(s)	968
PdmI	GAANNNNTTC	1 cut(s)	1310
PfeI	GAWTC	5 cut(s)	753, 904, 1154, 1255, 1411
Pfl23II	CGTACG	1 cut(s)	641
PflMI	CCANNNNNTGG	1 cut(s)	1529
PinAI	ACCGGT	1 cut(s)	520
PkrI	GCNGC	3 cut(s)	659, 1053, 1351
Ple19I	CGATCG	1 cut(s)	832
PleI	GAGTC	1 cut(s)	318
PmaCI	CACGTG	2 cut(s)	1094, 1238
PmlI	CACGTG	2 cut(s)	1094, 1238
PpsI	GAGTC	1 cut(s)	318
Ppu21I	YACGTR	2 cut(s)	1094, 1238
PpuMI	RGGWCCY	2 cut(s)	143, 1231
Psp124BI	GAGCTC	2 cut(s)	15, 1218
Psp5II	RGGWCCY	2 cut(s)	143, 1231
Psp6I	CCWGG	1 cut(s)	742
PspCI	CACGTG	2 cut(s)	1094, 1238
PspGI	CCWGG	1 cut(s)	742
PspLI	CGTACG	1 cut(s)	641
PspN4I	GGNNCC	6 cut(s)	145, 611, 1233, 1243, 1391, 1479
PspPI	GGNCC	8 cut(s)	99, 143, 311, 1231, 1347, 1390, 1477, 1598
PspPPI	RGGWCCY	2 cut(s)	143, 1231
PvuI	CGATCG	1 cut(s)	832
RruI	TCGCGA	1 cut(s)	1184
RsaI	GTAC	4 cut(s)	643, 1169, 1506, 1536
RsaNI	GTAC	4 cut(s)	642, 1168, 1505, 1535
SacI	GAGCTC	2 cut(s)	15, 1218
SapI	GCTCTTC	1 cut(s)	20
SaqAI	TTAA	3 cut(s)	50, 411, 1128
SatI	GCNGC	3 cut(s)	658, 1052, 1350
Sau96I	GGNCC	8 cut(s)	99, 143, 311, 1231, 1347, 1390, 1477, 1598
SchI	GAGTC	1 cut(s)	318
ScrFI	CCNGG	4 cut(s)	744, 1319, 1320, 1361
SduI	GDGCHC	3 cut(s)	15, 463, 1218
SfaNI	GCATC	5 cut(s)	709, 747, 1291, 1410, 1500
SfcI	CTRYAG	2 cut(s)	315, 1055
SfuI	TTCGAA	1 cut(s)	848
SinI	GGWCC	4 cut(s)	143, 311, 1231, 1390
SmaI	CCCGGG	1 cut(s)	1320
SmlI	CTYRAG	1 cut(s)	1127
SmoI	CTYRAG	1 cut(s)	1127
SseBI	AGGCCT	2 cut(s)	203, 911
SsiI	CCGC	6 cut(s)	80, 983, 1195, 1331, 1350, 1393
SspI	AATATT	1 cut(s)	360
SspMI	CTAG	5 cut(s)	246, 893, 1031, 1415, 1436
SstI	GAGCTC	2 cut(s)	15, 1218
StuI	AGGCCT	2 cut(s)	203, 911
StyD4I	CCNGG	4 cut(s)	742, 1317, 1318, 1359
TaaI	ACNGT	7 cut(s)	155, 319, 331, 943, 1047, 1326, 1504
TaiI	ACGT	3 cut(s)	549, 1096, 1240
TaqI	TCGA	7 cut(s)	322, 368, 828, 832, 848, 1199, 1588
TauI	GCSGC	1 cut(s)	1352
TfiI	GAWTC	5 cut(s)	753, 904, 1154, 1255, 1411
Tru1I	TTAA	3 cut(s)	50, 411, 1128
Tru9I	TTAA	3 cut(s)	50, 411, 1128
TscAI	CASTG	3 cut(s)	275, 946, 1052
TseFI	GTSAC	1 cut(s)	1094
TseI	GCWGC	2 cut(s)	657, 1051
Tsp45I	GTSAC	1 cut(s)	1094
TspDTI	ATGAA	4 cut(s)	151, 885, 1167, 1360
TspMI	CCCGGG	1 cut(s)	1318
TspRI	CASTG	3 cut(s)	275, 946, 1052
Van91I	CCANNNNNTGG	1 cut(s)	1529
Vha464I	CTTAAG	1 cut(s)	1127
VpaK11BI	GGWCC	4 cut(s)	143, 311, 1231, 1390
XapI	RAATTY	5 cut(s)	32, 377, 778, 844, 916
XceI	RCATGY	2 cut(s)	1401, 1496
XcmI	CCANNNNNNNNNTGG	2 cut(s)	49, 1360
XmaI	CCCGGG	1 cut(s)	1318
XmiI	GTMKAC	1 cut(s)	543
XmnI	GAANNNNTTC	1 cut(s)	1310
XspI	CTAG	5 cut(s)	246, 893, 1031, 1415, 1436
Zsp2I	ATGCAT	1 cut(s)	1403

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.