pycom02g03060

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0016060
Physical Location & Seq
Reverse (-)
1978463 .. 1980677
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g03060.1

Sequence Viewer

Length: 1251 bp
ATGGAGAATCTCAAGTTGTTGATACCGGTGATGGCAACGAGTATAGTAGACATGATGGACAAATGGTCAGCAATGTCCAGCTCCGGTGAGGTCGAGATTGAAGTATCCGAGTGGTTCCAAAACCTAACCGAAGATATAATTACCCGTACGGCGTTCGGAAGCAGCTACGAAGATGGCAAAGCCGTTTTCCGGCTACAAGCTCAGCAAATGGCGTTTGTTACCGAGGCCTTTCAAAAAGTTTCCATCCCTGGTTACAGATTCTTGCCCACAAAACGAAACATAAATTCTTGGAAATTGGATAAGCAAATCAGGAAGTCACTGATGAAGCTGATCGATCGACGGGTAGAGAATTCGAACTCGGGCGTGCATGAAAAATGCCCGAAAGATTTACTAGGTCTTATGATTCAGGCCTTAAATTCTTCTCCTTCTTCCAACATCACTGTCAACGACATTGTTGAAGAATGCAAGAGCTTCTTCTTTGCGGGCAAAGATACCACATCCAATTTGCTGACGTGGACAACGGTTCTCCTAGCAATGCACCCACTGTGGCAGCTACAGGCACGTGACGAGGTGCTGAGGGTGTGTGGATCACGTGACATACCCACTAAAGACGACGTTGTAAAGCTTAAGACGTTGAATATGGTCGTCAACGAATCACTACGTTTGTACCCGCCTGCGATCGCAACGATTCGACGGTCGAGAACAGAAGTGCAGCTCGGGGGATACACTGTCCCACGTGGGACCGAGCTTTTGATTCCAATCATAGCCGTTCATCACGATCAGAGCATATGGGGGAATGATGCGAAGGAGTTCAACCCGAGCCGGTTCGGAGAGGGCGTGGCGCGGGCCGCGAAGCATCCAGTGGCGTTCCTTCCATTCGGGCTTGGGGTCCGCACATGCATCGGGCAGAATCTAGCATTGTTGCAGGCTAAACTAGCCCTTGCCATCATGCTTCAACGCTTCTCTTTCAGGCTGGCCCCTACCTATCAACATGCACCTACCGTCCTGATGCTACTTCACCCCCAATACGGTGCACCCATCATTTTCCAACGCCTGCCACCACATGATCAGGGTCAAACTTCTCTGATCGAAATCAAGGACCTTGATCTCTTACTGTCACTATGCAATACCAGTCGTTTGCGTGTGAGTGTGCCGTATGATTCGGATGAATTGACCGAAGTACCCATTTGGCCGATTTTCCTTATTTTGTTTTCTTATTCATTTTTATGCTGGGTTTCCTTAAAACTATAG

Protein Analysis

417

Amino Acids

46.96

Weight (kDa)

8.44

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 7 - 331 2.2e-68 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26710
fragaria_vesca FvH4_1g03910
malus_domestica MD02G1037300.v1.1 MD15G1177600.v1.1
prunus_persica Prupe.7G237900_v2.0.a1
pyrus_communis pycom02g03060 pycom15g15890
rosa_chinensis RchiOBHm_Chr2g0089381
rosa_laevigata RLG00000016022
rosa_multiflora Rmu_sc0000197.1_g000024
rosa_roxburghii Rroxscaffold_2G00152010
rosa_rugosa Rorug01G0490300
rosa_samantha Rh2AG045400 Rh2DG045300
rosa_wichuraiana Rw2G003730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 48
AccII CGCG 2 cut(s) 844, 851
AciI CCGC 5 cut(s) 482, 671, 844, 849, 892
AclWI GGATC 1 cut(s) 595
AcoI YGGCCR 1 cut(s) 1190
AcsI RAATTY 3 cut(s) 283, 349, 415
AcvI CACGTG 3 cut(s) 563, 593, 737
AfaI GTAC 3 cut(s) 148, 668, 1182
AfiI CCNNNNNNNGG 2 cut(s) 189, 1028
AflII CTTAAG 1 cut(s) 626
AgeI ACCGGT 1 cut(s) 25
AgsI TTSAA 6 cut(s) 101, 233, 458, 637, 814, 956
AhdI GACNNNNNGTC 1 cut(s) 64
AjiI CACGTC 1 cut(s) 513
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 9 cut(s) 81, 165, 200, 328, 471, 553, 625, 715, 748
AluI AGCT 9 cut(s) 81, 165, 200, 328, 471, 553, 625, 715, 748
Alw21I GWGCWC 1 cut(s) 1036
Alw44I GTGCAC 1 cut(s) 1032
AlwI GGATC 1 cut(s) 595
Ama87I CYCGRG 3 cut(s) 358, 716, 817
AoxI GGCC 5 cut(s) 225, 408, 846, 975, 1190
ApaLI GTGCAC 1 cut(s) 1032
ApeKI GCWGC 3 cut(s) 162, 550, 712
ApoI RAATTY 3 cut(s) 283, 349, 415
ArsI GACNNNNNNTTYG 2 cut(s) 733, 765
AsiGI ACCGGT 1 cut(s) 25
AsiSI GCGATCGC 1 cut(s) 681
Asp700I GAANNNNTTC 1 cut(s) 809
AspLEI GCGC 1 cut(s) 844
AspS9I GGNCC 5 cut(s) 741, 846, 889, 976, 1099
AsuHPI GGTGA 3 cut(s) 40, 98, 1010
AsuII TTCGAA 1 cut(s) 353
AvaI CYCGRG 3 cut(s) 358, 716, 817
AvaII GGWCC 3 cut(s) 741, 889, 1099
BaeGI GKGCMC 1 cut(s) 1036
BaeI ACNNNNGTAYC 4 cut(s) 650, 683, 1164, 1197
BbrPI CACGTG 3 cut(s) 563, 593, 737
Bbv12I GWGCWC 1 cut(s) 1036
BbvCI CCTCAGC 1 cut(s) 575
BbvI GCAGC 3 cut(s) 174, 562, 724
BccI CCATC 6 cut(s) 25, 49, 167, 251, 953, 1046
BceAI ACGGC 4 cut(s) 165, 167, 752, 1138
BcgI CGANNNNNNTGC 2 cut(s) 883, 917
BciT130I CCWGG 1 cut(s) 249
BciVI GTATCC 2 cut(s) 115, 716
BclI TGATCA 1 cut(s) 1066
BfaI CTAG 4 cut(s) 392, 530, 914, 935
BfmI CTRYAG 2 cut(s) 554, 1247
BfrI CTTAAG 1 cut(s) 626
BfuI GTATCC 2 cut(s) 115, 716
BisI GCNGC 4 cut(s) 163, 551, 713, 849
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 4 cut(s) 164, 552, 714, 850
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 3 cut(s) 741, 889, 1099
BmeRI GACNNNNNGTC 1 cut(s) 64
BmeT110I CYCGRG 3 cut(s) 358, 716, 817
BmgBI CACGTC 1 cut(s) 513
BmgT120I GGNCC 5 cut(s) 741, 846, 889, 976, 1099
BmiI GGNNCC 4 cut(s) 116, 742, 890, 978
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 4 cut(s) 790, 865, 909, 999
Bpu10I CCTNAGC 1 cut(s) 575
Bpu1102I GCTNAGC 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 353
Bsa29I ATCGAT 1 cut(s) 333
BsaAI YACGTR 3 cut(s) 563, 593, 737
BsaBI GATNNNNATC 2 cut(s) 758, 1091
BsaJI CCNNGG 2 cut(s) 222, 247
BsaWI WCCGGW 2 cut(s) 25, 83
BsaXI ACNNNNNCTCC 2 cut(s) 822, 852
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 1028
Bse118I RCCGGY 2 cut(s) 25, 822
Bse1I ACTGG 2 cut(s) 860, 1131
Bse3DI GCAATG 2 cut(s) 78, 540
Bse8I GATNNNNATC 2 cut(s) 758, 1091
BseBI CCWGG 1 cut(s) 249
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 222, 247
BseGI GGATG 4 cut(s) 243, 497, 856, 1171
BseJI GATNNNNATC 2 cut(s) 758, 1091
BseLI CCNNNNNNNGG 2 cut(s) 189, 1028
BseMI GCAATG 2 cut(s) 78, 540
BseMII CTCAG 2 cut(s) 215, 566
BseNI ACTGG 2 cut(s) 860, 1131
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 3 cut(s) 174, 562, 724
BseYI CCCAGC 1 cut(s) 1230
BsgI GTGCAG 1 cut(s) 731
Bsh1236I CGCG 2 cut(s) 844, 851
Bsh1285I CGRYCG 3 cut(s) 337, 681, 698
BshFI GGCC 5 cut(s) 227, 410, 848, 977, 1192
BshTI ACCGGT 1 cut(s) 25
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 3 cut(s) 337, 681, 698
BsiHKAI GWGCWC 1 cut(s) 1036
BsiHKCI CYCGRG 3 cut(s) 358, 716, 817
BsiSI CCGG 4 cut(s) 26, 84, 190, 823
BsiWI CGTACG 1 cut(s) 146
BslFI GGGAC 2 cut(s) 716, 754
BslI CCNNNNNNNGG 2 cut(s) 189, 1028
BsmFI GGGAC 2 cut(s) 716, 754
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 5 cut(s) 227, 410, 848, 977, 1192
BsoBI CYCGRG 3 cut(s) 358, 716, 817
Bsp119I TTCGAA 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 1036
Bsp143I GATC 8 cut(s) 330, 334, 587, 678, 778, 1066, 1086, 1105
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 5 cut(s) 482, 671, 844, 849, 892
BspANI GGCC 5 cut(s) 227, 410, 848, 977, 1192
BspCNI CTCAG 2 cut(s) 214, 567
BspDI ATCGAT 1 cut(s) 333
BspFNI CGCG 2 cut(s) 844, 851
BspLI GGNNCC 4 cut(s) 116, 742, 890, 978
BspPI GGATC 1 cut(s) 595
BspT104I TTCGAA 1 cut(s) 353
BspTI CTTAAG 1 cut(s) 626
BsrDI GCAATG 2 cut(s) 78, 540
BsrFI RCCGGY 2 cut(s) 25, 822
BsrI ACTGG 2 cut(s) 860, 1131
BssAI RCCGGY 2 cut(s) 25, 822
BssECI CCNNGG 2 cut(s) 222, 247
BssMI GATC 8 cut(s) 330, 334, 587, 678, 778, 1066, 1086, 1105
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 8 cut(s) 442, 523, 546, 696, 730, 1003, 1031, 1116
BstAFI CTTAAG 1 cut(s) 626
BstBAI YACGTR 3 cut(s) 563, 593, 737
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 7 cut(s) 365, 484, 675, 846, 927, 975, 1055
BstDEI CTNAG 2 cut(s) 201, 575
BstF5I GGATG 4 cut(s) 243, 497, 856, 1171
BstFNI CGCG 2 cut(s) 844, 851
BstHHI GCGC 1 cut(s) 844
BstKTI GATC 8 cut(s) 333, 337, 590, 681, 781, 1069, 1089, 1108
BstMBI GATC 8 cut(s) 330, 334, 587, 678, 778, 1066, 1086, 1105
BstMCI CGRYCG 3 cut(s) 337, 681, 698
BstMWI GCNNNNNNNGC 2 cut(s) 848, 935
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 2 cut(s) 900, 995
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 2 cut(s) 554, 1247
BstSLI GKGCMC 1 cut(s) 1036
BstUI CGCG 2 cut(s) 844, 851
BstV1I GCAGC 3 cut(s) 174, 562, 724
Bsu15I ATCGAT 1 cut(s) 333
BsuI GTATCC 2 cut(s) 115, 716
BsuRI GGCC 5 cut(s) 227, 410, 848, 977, 1192
BsuTUI ATCGAT 1 cut(s) 333
BtrI CACGTC 1 cut(s) 513
BtsCI GGATG 4 cut(s) 243, 497, 856, 1171
BtsIMutI CAGTG 5 cut(s) 317, 438, 542, 726, 867
Cac8I GCNNGC 7 cut(s) 365, 484, 675, 846, 927, 975, 1055
CfoI GCGC 1 cut(s) 844
Cfr10I RCCGGY 2 cut(s) 25, 822
Cfr13I GGNCC 5 cut(s) 741, 846, 889, 976, 1099
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 3 cut(s) 147, 667, 1181
CspAI ACCGGT 1 cut(s) 25
CviAII CATG 6 cut(s) 52, 368, 897, 949, 992, 1064
CviQI GTAC 3 cut(s) 147, 667, 1181
DdeI CTNAG 2 cut(s) 201, 575
DpnI GATC 8 cut(s) 332, 336, 589, 680, 780, 1068, 1088, 1107
DpnII GATC 8 cut(s) 330, 334, 587, 678, 778, 1066, 1086, 1105
DriI GACNNNNNGTC 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 1190
Eam1105I GACNNNNNGTC 1 cut(s) 64
Eco147I AGGCCT 2 cut(s) 227, 410
Eco47I GGWCC 3 cut(s) 741, 889, 1099
Eco72I CACGTG 3 cut(s) 563, 593, 737
Eco88I CYCGRG 3 cut(s) 358, 716, 817
EcoO109I RGGNCCY 1 cut(s) 1099
EcoRI GAATTC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 902
FaeI CATG 6 cut(s) 55, 371, 900, 952, 995, 1067
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 2 cut(s) 716, 754
FatI CATG 6 cut(s) 51, 367, 896, 948, 991, 1063
FauI CCCGC 3 cut(s) 475, 678, 837
FauNDI CATATG 1 cut(s) 788
FbaI TGATCA 1 cut(s) 1066
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 4 cut(s) 163, 551, 713, 849
FokI GGATG 4 cut(s) 230, 484, 843, 1178
Fsp4HI GCNGC 4 cut(s) 163, 551, 713, 849
FspBI CTAG 4 cut(s) 392, 530, 914, 935
GlaI GCGC 1 cut(s) 843
GluI GCNGC 4 cut(s) 163, 551, 713, 849
GsaI CCCAGC 1 cut(s) 1234
HaeIII GGCC 5 cut(s) 227, 410, 848, 977, 1192
HapII CCGG 4 cut(s) 26, 84, 190, 823
HhaI GCGC 1 cut(s) 844
Hin1II CATG 6 cut(s) 55, 371, 900, 952, 995, 1067
Hin6I GCGC 1 cut(s) 842
HinP1I GCGC 1 cut(s) 842
HincII GTYRAC 2 cut(s) 445, 649
HindII GTYRAC 2 cut(s) 445, 649
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 8 cut(s) 7, 258, 403, 653, 688, 754, 910, 1160
HpaII CCGG 4 cut(s) 26, 84, 190, 823
HphI GGTGA 3 cut(s) 40, 98, 1010
Hpy166II GTNNAC 5 cut(s) 49, 445, 516, 649, 1034
Hpy188I TCNGA 6 cut(s) 109, 158, 783, 830, 1086, 1165
Hpy188III TCNNGA 5 cut(s) 94, 310, 699, 776, 1006
Hpy8I GTNNAC 5 cut(s) 49, 445, 516, 649, 1034
Hpy99I CGWCG 3 cut(s) 342, 617, 696
HpyAV CCTTC 3 cut(s) 435, 799, 881
HpyCH4III ACNGT 8 cut(s) 442, 523, 546, 696, 730, 1003, 1031, 1116
HpyCH4IV ACGT 7 cut(s) 512, 562, 592, 615, 632, 661, 736
HpyCH4V TGCA 9 cut(s) 367, 465, 538, 712, 900, 925, 995, 1034, 1125
HpyF10VI GCNNNNNNNGC 2 cut(s) 848, 935
HpyF3I CTNAG 2 cut(s) 201, 575
HpySE526I ACGT 7 cut(s) 512, 562, 592, 615, 632, 661, 736
Hsp92II CATG 6 cut(s) 55, 371, 900, 952, 995, 1067
HspAI GCGC 1 cut(s) 842
Ksp22I TGATCA 1 cut(s) 1066
Kzo9I GATC 8 cut(s) 330, 334, 587, 678, 778, 1066, 1086, 1105
LmnI GCTCC 1 cut(s) 86
Lsp1109I GCAGC 3 cut(s) 174, 562, 724
LweI GCATC 4 cut(s) 790, 865, 909, 999
MaeI CTAG 4 cut(s) 392, 530, 914, 935
MaeII ACGT 7 cut(s) 512, 562, 592, 615, 632, 661, 736
MaeIII GTNAC 6 cut(s) 217, 251, 315, 563, 593, 1116
MalI GATC 8 cut(s) 332, 336, 589, 680, 780, 1068, 1088, 1107
MboI GATC 8 cut(s) 330, 334, 587, 678, 778, 1066, 1086, 1105
MboII GAAGA 6 cut(s) 143, 182, 411, 420, 466, 470
MhlI GDGCHC 1 cut(s) 1036
MluCI AATT 7 cut(s) 138, 283, 293, 349, 415, 502, 1169
MmeI TCCRAC 2 cut(s) 456, 1072
MnlI CCTC 5 cut(s) 82, 217, 562, 570, 826
Mph1103I ATGCAT 1 cut(s) 902
MroXI GAANNNNTTC 1 cut(s) 809
MseI TTAA 3 cut(s) 413, 627, 1241
MslI CAYNNNNRTG 1 cut(s) 1225
MspCI CTTAAG 1 cut(s) 626
MspI CCGG 4 cut(s) 26, 84, 190, 823
MspR9I CCNGG 1 cut(s) 249
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 2 cut(s) 844, 851
MwoI GCNNNNNNNGC 2 cut(s) 848, 935
NdeI CATATG 1 cut(s) 788
NdeII GATC 8 cut(s) 330, 334, 587, 678, 778, 1066, 1086, 1105
NlaIII CATG 6 cut(s) 55, 371, 900, 952, 995, 1067
NlaIV GGNNCC 4 cut(s) 116, 742, 890, 978
NmuCI GTSAC 4 cut(s) 315, 563, 593, 1116
NsiI ATGCAT 1 cut(s) 902
NspI RCATGY 2 cut(s) 900, 995
NspV TTCGAA 1 cut(s) 353
PceI AGGCCT 2 cut(s) 227, 410
PcsI WCGNNNNNNNCGW 1 cut(s) 834
PctI GAATGC 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 809
PfeI GAWTC 8 cut(s) 7, 258, 403, 653, 688, 754, 910, 1160
Pfl23II CGTACG 1 cut(s) 146
PinAI ACCGGT 1 cut(s) 25
PkrI GCNGC 4 cut(s) 164, 552, 714, 850
Ple19I CGATCG 2 cut(s) 337, 681
PmaCI CACGTG 3 cut(s) 563, 593, 737
PmlI CACGTG 3 cut(s) 563, 593, 737
Ppu21I YACGTR 3 cut(s) 563, 593, 737
PpuMI RGGWCCY 1 cut(s) 1099
Psp5II RGGWCCY 1 cut(s) 1099
Psp6I CCWGG 1 cut(s) 247
PspCI CACGTG 3 cut(s) 563, 593, 737
PspFI CCCAGC 1 cut(s) 1230
PspGI CCWGG 1 cut(s) 247
PspLI CGTACG 1 cut(s) 146
PspN4I GGNNCC 4 cut(s) 116, 742, 890, 978
PspPI GGNCC 5 cut(s) 741, 846, 889, 976, 1099
PspPPI RGGWCCY 1 cut(s) 1099
PvuI CGATCG 2 cut(s) 337, 681
RgaI GCGATCGC 1 cut(s) 681
RsaI GTAC 3 cut(s) 148, 668, 1182
RsaNI GTAC 3 cut(s) 147, 667, 1181
RseI CAYNNNNRTG 1 cut(s) 1225
SaqAI TTAA 3 cut(s) 413, 627, 1241
SatI GCNGC 4 cut(s) 163, 551, 713, 849
Sau3AI GATC 8 cut(s) 330, 334, 587, 678, 778, 1066, 1086, 1105
Sau96I GGNCC 5 cut(s) 741, 846, 889, 976, 1099
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 1 cut(s) 1036
SfaAI GCGATCGC 1 cut(s) 681
SfaNI GCATC 4 cut(s) 790, 865, 909, 999
SfcI CTRYAG 2 cut(s) 554, 1247
SfuI TTCGAA 1 cut(s) 353
SgfI GCGATCGC 1 cut(s) 681
SinI GGWCC 3 cut(s) 741, 889, 1099
SmiMI CAYNNNNRTG 1 cut(s) 1225
SmlI CTYRAG 2 cut(s) 11, 626
SmoI CTYRAG 2 cut(s) 11, 626
Sse9I AATT 7 cut(s) 138, 283, 293, 349, 415, 502, 1169
SseBI AGGCCT 2 cut(s) 227, 410
SsiI CCGC 5 cut(s) 482, 671, 844, 849, 892
SspMI CTAG 4 cut(s) 392, 530, 914, 935
StuI AGGCCT 2 cut(s) 227, 410
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 8 cut(s) 442, 523, 546, 696, 730, 1003, 1031, 1116
TaiI ACGT 7 cut(s) 515, 565, 595, 618, 635, 664, 739
TaqI TCGA 7 cut(s) 93, 333, 337, 353, 691, 698, 1089
TaqII GACCGA 2 cut(s) 758, 1190
TasI AATT 7 cut(s) 138, 283, 293, 349, 415, 502, 1169
TauI GCSGC 1 cut(s) 851
TfiI GAWTC 8 cut(s) 7, 258, 403, 653, 688, 754, 910, 1160
Tru1I TTAA 3 cut(s) 413, 627, 1241
Tru9I TTAA 3 cut(s) 413, 627, 1241
TscAI CASTG 5 cut(s) 324, 445, 549, 733, 867
TseFI GTSAC 4 cut(s) 315, 563, 593, 1116
TseI GCWGC 3 cut(s) 162, 550, 712
Tsp45I GTSAC 4 cut(s) 315, 563, 593, 1116
TspDTI ATGAA 5 cut(s) 338, 384, 761, 1182, 1209
TspRI CASTG 5 cut(s) 324, 445, 549, 733, 867
Vha464I CTTAAG 1 cut(s) 626
VneI GTGCAC 1 cut(s) 1032
VpaK11BI GGWCC 3 cut(s) 741, 889, 1099
XapI RAATTY 3 cut(s) 283, 349, 415
XceI RCATGY 2 cut(s) 900, 995
XmiI GTMKAC 1 cut(s) 48
XmnI GAANNNNTTC 1 cut(s) 809
XspI CTAG 4 cut(s) 392, 530, 914, 935
Zsp2I ATGCAT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.