Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0013975

Physical Location & Seq

Genomic Coordinates Forward (+)

16567617 .. 16572202

Gene Structure 4586 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1208200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1227 bp

ATGGTGTTATTCACAACCGGGACAAGTGTACTCCTACCCGCACTCGGGGATATCTCTGGCTTCCTTCGAAATGATACCGAATCCATCAATTCAACCTCAACCGATTATGGCCATATAGTTTATCAATACGCATCTGCAGTTCTTAATCCAGCGTCGCCAAATGACATCGCAAGTTTAATCCTATCCGCAAACAACAATTCTGCGCCTTTCAGTGTAGCTGCTAGAGGCCAGGGCCATTCTGTTCGTGGACAGGCCCTGACCTCTGATGGCGTTGTCATAAACATGACAGCTTTGATCAGTAATGGGTCTAGAATTGTAGTCTTTGAGAGCCCTTCTTTGGGTTCTTATGCTGATGTTGGAGGTGAGCAGCGTTGGATCGATGTGTTGCGTGCTACACTCGACCGTGGTCTTACTCCAGTTTCATGGACTGACTACCTCTACCTCAGCGTTGGTGGCACGCTCAGTAACGCCGGAATCAGTGGGCAAACGTTTCGTTATGGACCTCAAATCAACAATGTCTATGAACTTGATGTTGTTACAGGGAAAGGGGAATTAGTTACATGCTCCTCAAACAAGGTGCCAGGGCTATTTTATGCTGTTCTCGGAGGCTTGGGCCAATTTGGTATAATAACCAGAGCAAGGATTCCCCTAGAGCCAGCTCCGAACAGGGCTAAATGGGTTCGAATGATGTACAGAAACTTCTCTGCATTTTCAAAAGATCAAGAACGTTTAATGGCAAACAACGGATTCGATTATTTAGAAGGTTTCCTTCTAATGAAGCAGGGTCCTCTAGACCTTTCCTTTTACCCATTACTCGATCAACCAAGAATAACAGCTTTAATAAATCAATACGGCATCATCTACTACATAGAAGCAGCTAAATATTATGGTGATAGCACTCGACCAACAGTGGACAAGATTTTCTGGATAGAGTGGGACGGTAGGATGTCTGCAGTTATTCCAGATGAAGATGTTTGCTATGTTGTAGGGCTCTTGCATGCAAGCGGATTCAATGACTGGCAGGCATTTGATGAAGTAAACAGACAAATACTGCAATTTTGTAATGATGCTGGCATTCTGGTTAAGCAGTACCTTCCCCACTATGAAACACAGGAGGATTGGAAAAACCATTTCGGAACAAAGTGGCAAAGTTTCCGAGACAGGAAAGTTAGGTTTGATCCAAACAAGATATTAGCACCAGGACAAAGGATTTTCAGTGTTTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0006725 GO:0006807 GO:0008131 GO:0008150 GO:0008152 GO:0009056 GO:0009308 GO:0009690 GO:0009823 GO:0009987 GO:0010817 GO:0012505 GO:0016491 GO:0016638 GO:0016641 GO:0016645 GO:0019139 GO:0031974 GO:0034754 GO:0042445 GO:0042447 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044421 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00908 map00908

Pfam Domains

Protein Families

Cytokin-bind FAD_binding_4

Protein Analysis

409

Amino Acids

45.4

Weight (kDa)

5.62

Isoelectric Point (pI)

33.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	45 - 189	7.7e-18	FAD binding domain
Cytokin-bind	PF09265	221 - 306	8.2e-18	Cytokinin dehydrogenase 1, FAD and cytokinin binding
Cytokin-bind	PF09265	311 - 405	3.3e-37	Cytokinin dehydrogenase 1, FAD and cytokinin binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19500 AT4G29740 AT4G29740 AT4G29740
fragaria_vesca	FvH4_1g07610
malus_domestica	MD02G1080700.v1.1 MD15G1208200.v1.1
prunus_persica	Prupe.7G208500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0093481
rosa_laevigata	RLG00000016410
rosa_multiflora	Rmu_sc0004086.1_g000001
rosa_roxburghii	Rroxscaffold_2G00148050
rosa_samantha	Rh2AG083100 Rh2BG083800 Rh2CG085800 Rh2DG081900
rosa_wichuraiana	Rw2G006430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	577
AciI	CCGC	3 cut(s)	39, 186, 1005
AclI	AACGTT	2 cut(s)	488, 727
AclWI	GGATC	2 cut(s)	383, 1172
AcoI	YGGCCR	1 cut(s)	109
AfaI	GTAC	3 cut(s)	30, 692, 1091
AfiI	CCNNNNNNNGG	5 cut(s)	44, 45, 337, 338, 639
AgsI	TTSAA	3 cut(s)	93, 714, 1012
AjnI	CCWGG	3 cut(s)	228, 580, 1198
AluBI	AGCT	5 cut(s)	218, 290, 659, 836, 878
AluI	AGCT	5 cut(s)	218, 290, 659, 836, 878
Alw26I	GTCTC	1 cut(s)	1152
AlwI	GGATC	2 cut(s)	383, 1172
AlwNI	CAGNNNCTG	1 cut(s)	256
Ama87I	CYCGRG	1 cut(s)	44
AoxI	GGCC	5 cut(s)	109, 226, 232, 252, 613
ApeKI	GCWGC	3 cut(s)	218, 367, 875
AspLEI	GCGC	1 cut(s)	205
AspS9I	GGNCC	5 cut(s)	232, 253, 500, 613, 785
AsuC2I	CCSGG	1 cut(s)	19
AsuHPI	GGTGA	2 cut(s)	374, 902
AsuII	TTCGAA	2 cut(s)	67, 682
AvaI	CYCGRG	1 cut(s)	44
AvaII	GGWCC	2 cut(s)	500, 785
BalI	TGGCCA	1 cut(s)	111
BanI	GGYRCC	1 cut(s)	577
BanII	GRGCYC	2 cut(s)	332, 993
BarI	GAAGNNNNNNTAC	2 cut(s)	683, 715
BbvCI	CCTCAGC	1 cut(s)	443
BbvI	GCAGC	3 cut(s)	205, 379, 887
BccI	CCATC	2 cut(s)	92, 260
BceAI	ACGGC	1 cut(s)	868
BcgI	CGANNNNNNTGC	2 cut(s)	473, 507
BciT130I	CCWGG	3 cut(s)	230, 582, 1200
BclI	TGATCA	1 cut(s)	294
BcnI	CCSGG	1 cut(s)	19
BcoDI	GTCTC	1 cut(s)	1152
BfaI	CTAG	4 cut(s)	222, 309, 650, 791
BfmI	CTRYAG	2 cut(s)	135, 951
BisI	GCNGC	3 cut(s)	219, 368, 876
BlsI	GCNGC	3 cut(s)	220, 369, 877
Bme1390I	CCNGG	4 cut(s)	19, 230, 582, 1200
Bme18I	GGWCC	2 cut(s)	500, 785
BmeT110I	CYCGRG	1 cut(s)	44
BmgT120I	GGNCC	5 cut(s)	232, 253, 500, 613, 785
BmiI	GGNNCC	2 cut(s)	579, 786
BmrFI	CCNGG	4 cut(s)	19, 230, 582, 1200
BmsI	GCATC	3 cut(s)	140, 864, 1057
BoxI	GACNNNNGTC	1 cut(s)	405
BpmI	CTGGAG	1 cut(s)	399
Bpu10I	CCTNAGC	1 cut(s)	443
Bpu14I	TTCGAA	2 cut(s)	67, 682
BpuMI	CCSGG	1 cut(s)	19
Bsa29I	ATCGAT	1 cut(s)	378
BsaJI	CCNNGG	3 cut(s)	229, 403, 581
Bsc4I	CCNNNNNNNGG	5 cut(s)	44, 45, 337, 338, 639
Bse1I	ACTGG	2 cut(s)	416, 1022
BseBI	CCWGG	3 cut(s)	230, 582, 1200
BseCI	ATCGAT	1 cut(s)	378
BseDI	CCNNGG	3 cut(s)	229, 403, 581
BseGI	GGATG	1 cut(s)	951
BseLI	CCNNNNNNNGG	5 cut(s)	44, 45, 337, 338, 639
BseMII	CTCAG	2 cut(s)	457, 475
BseNI	ACTGG	2 cut(s)	416, 1022
BseRI	GAGGAG	1 cut(s)	556
BseXI	GCAGC	3 cut(s)	205, 379, 887
Bsh1285I	CGRYCG	1 cut(s)	403
BshFI	GGCC	5 cut(s)	111, 228, 234, 254, 615
BshNI	GGYRCC	1 cut(s)	577
BshVI	ATCGAT	1 cut(s)	378
BsiEI	CGRYCG	1 cut(s)	403
BsiHKCI	CYCGRG	1 cut(s)	44
BsiSI	CCGG	2 cut(s)	18, 471
BslFI	GGGAC	2 cut(s)	34, 950
BslI	CCNNNNNNNGG	5 cut(s)	44, 45, 337, 338, 639
BsmAI	GTCTC	1 cut(s)	1152
BsmFI	GGGAC	2 cut(s)	34, 950
BsmI	GAATGC	1 cut(s)	1074
BsnI	GGCC	5 cut(s)	111, 228, 234, 254, 615
BsoBI	CYCGRG	1 cut(s)	44
Bsp119I	TTCGAA	2 cut(s)	67, 682
Bsp1286I	GDGCHC	2 cut(s)	332, 993
Bsp1407I	TGTACA	1 cut(s)	690
Bsp143I	GATC	5 cut(s)	294, 375, 718, 817, 1177
BspACI	CCGC	3 cut(s)	39, 186, 1005
BspANI	GGCC	5 cut(s)	111, 228, 234, 254, 615
BspCNI	CTCAG	2 cut(s)	456, 474
BspDI	ATCGAT	1 cut(s)	378
BspLI	GGNNCC	2 cut(s)	579, 786
BspMAI	CTGCAG	2 cut(s)	139, 955
BspPI	GGATC	2 cut(s)	383, 1172
BspT104I	TTCGAA	2 cut(s)	67, 682
BspT107I	GGYRCC	1 cut(s)	577
BsrGI	TGTACA	1 cut(s)	690
BsrI	ACTGG	2 cut(s)	416, 1022
BssECI	CCNNGG	3 cut(s)	229, 403, 581
BssMI	GATC	5 cut(s)	294, 375, 718, 817, 1177
Bst2UI	CCWGG	3 cut(s)	230, 582, 1200
Bst4CI	ACNGT	3 cut(s)	404, 910, 941
BstAUI	TGTACA	1 cut(s)	690
BstBI	TTCGAA	2 cut(s)	67, 682
BstC8I	GCNNGC	7 cut(s)	390, 458, 657, 999, 1003, 1023, 1072
BstDEI	CTNAG	2 cut(s)	443, 461
BstDSI	CCRYGG	1 cut(s)	403
BstF5I	GGATG	1 cut(s)	951
BstHHI	GCGC	1 cut(s)	205
BstKTI	GATC	5 cut(s)	297, 378, 721, 820, 1180
BstMAI	GTCTC	1 cut(s)	1152
BstMBI	GATC	5 cut(s)	294, 375, 718, 817, 1177
BstMCI	CGRYCG	1 cut(s)	403
BstMWI	GCNNNNNNNGC	1 cut(s)	453
BstNI	CCWGG	3 cut(s)	230, 582, 1200
BstNSI	RCATGY	2 cut(s)	564, 1001
BstPAI	GACNNNNGTC	1 cut(s)	405
BstSCI	CCNGG	4 cut(s)	17, 228, 580, 1198
BstSFI	CTRYAG	2 cut(s)	135, 951
BstV1I	GCAGC	3 cut(s)	205, 379, 887
BstXI	CCANNNNNNTGG	1 cut(s)	423
Bsu15I	ATCGAT	1 cut(s)	378
BsuRI	GGCC	5 cut(s)	111, 228, 234, 254, 615
BsuTUI	ATCGAT	1 cut(s)	378
BtgI	CCRYGG	1 cut(s)	403
BtgZI	GCGATG	1 cut(s)	151
BtsCI	GGATG	1 cut(s)	951
BtsIMutI	CAGTG	4 cut(s)	217, 484, 915, 1222
Cac8I	GCNNGC	7 cut(s)	390, 458, 657, 999, 1003, 1023, 1072
CaiI	CAGNNNCTG	1 cut(s)	256
CfoI	GCGC	1 cut(s)	205
Cfr13I	GGNCC	5 cut(s)	232, 253, 500, 613, 785
ClaI	ATCGAT	1 cut(s)	378
CseI	GACGC	1 cut(s)	141
Csp6I	GTAC	3 cut(s)	29, 691, 1090
CviAII	CATG	4 cut(s)	283, 423, 561, 998
CviQI	GTAC	3 cut(s)	29, 691, 1090
DdeI	CTNAG	2 cut(s)	443, 461
DpnI	GATC	5 cut(s)	296, 377, 720, 819, 1179
DpnII	GATC	5 cut(s)	294, 375, 718, 817, 1177
EaeI	YGGCCR	1 cut(s)	109
Eco24I	GRGCYC	2 cut(s)	332, 993
Eco32I	GATATC	1 cut(s)	52
Eco47I	GGWCC	2 cut(s)	500, 785
Eco88I	CYCGRG	1 cut(s)	44
EcoO109I	RGGNCCY	2 cut(s)	253, 785
EcoRII	CCWGG	3 cut(s)	228, 580, 1198
EcoRV	GATATC	1 cut(s)	52
EcoT38I	GRGCYC	2 cut(s)	332, 993
FaeI	CATG	4 cut(s)	286, 426, 564, 1001
FalI	AAGNNNNNCTT	2 cut(s)	753, 785
FaqI	GGGAC	2 cut(s)	34, 950
FatI	CATG	4 cut(s)	282, 422, 560, 997
FauI	CCCGC	1 cut(s)	46
FbaI	TGATCA	1 cut(s)	294
Fnu4HI	GCNGC	3 cut(s)	219, 368, 876
FokI	GGATG	1 cut(s)	958
FriOI	GRGCYC	2 cut(s)	332, 993
Fsp4HI	GCNGC	3 cut(s)	219, 368, 876
FspBI	CTAG	4 cut(s)	222, 309, 650, 791
GlaI	GCGC	1 cut(s)	204
GluI	GCNGC	3 cut(s)	219, 368, 876
GsuI	CTGGAG	1 cut(s)	399
HaeIII	GGCC	5 cut(s)	111, 228, 234, 254, 615
HapII	CCGG	2 cut(s)	18, 471
HgaI	GACGC	1 cut(s)	141
HhaI	GCGC	1 cut(s)	205
Hin1II	CATG	4 cut(s)	286, 426, 564, 1001
Hin6I	GCGC	1 cut(s)	203
HinP1I	GCGC	1 cut(s)	203
HinfI	GANTC	5 cut(s)	80, 474, 643, 747, 1008
HpaII	CCGG	2 cut(s)	18, 471
HphI	GGTGA	2 cut(s)	374, 902
Hpy166II	GTNNAC	4 cut(s)	29, 248, 913, 1039
Hpy188I	TCNGA	5 cut(s)	265, 605, 663, 1136, 1157
Hpy188III	TCNNGA	5 cut(s)	309, 722, 791, 925, 962
Hpy8I	GTNNAC	4 cut(s)	29, 248, 913, 1039
Hpy99I	CGWCG	1 cut(s)	157
HpyAV	CCTTC	5 cut(s)	74, 342, 755, 779, 1103
HpyCH4III	ACNGT	3 cut(s)	404, 910, 941
HpyCH4IV	ACGT	2 cut(s)	488, 727
HpyCH4V	TGCA	6 cut(s)	137, 707, 953, 997, 1001, 1054
HpyF10VI	GCNNNNNNNGC	1 cut(s)	453
HpyF3I	CTNAG	2 cut(s)	443, 461
HpySE526I	ACGT	2 cut(s)	488, 727
Hsp92II	CATG	4 cut(s)	286, 426, 564, 1001
HspAI	GCGC	1 cut(s)	203
Ksp22I	TGATCA	1 cut(s)	294
Kzo9I	GATC	5 cut(s)	294, 375, 718, 817, 1177
LmnI	GCTCC	2 cut(s)	569, 664
Lsp1109I	GCAGC	3 cut(s)	205, 379, 887
LweI	GCATC	3 cut(s)	140, 864, 1057
MaeI	CTAG	4 cut(s)	222, 309, 650, 791
MaeII	ACGT	2 cut(s)	488, 727
MaeIII	GTNAC	3 cut(s)	464, 535, 556
MalI	GATC	5 cut(s)	296, 377, 720, 819, 1179
MboI	GATC	5 cut(s)	294, 375, 718, 817, 1177
MboII	GAAGA	1 cut(s)	980
MhlI	GDGCHC	2 cut(s)	332, 993
MlsI	TGGCCA	1 cut(s)	111
MluCI	AATT	6 cut(s)	88, 196, 312, 551, 617, 1055
MluNI	TGGCCA	1 cut(s)	111
MmeI	TCCRAC	2 cut(s)	337, 353
Mox20I	TGGCCA	1 cut(s)	111
MscI	TGGCCA	1 cut(s)	111
MseI	TTAA	6 cut(s)	144, 176, 731, 839, 1083, 1225
MslI	CAYNNNNRTG	1 cut(s)	281
Msp20I	TGGCCA	1 cut(s)	111
MspI	CCGG	2 cut(s)	18, 471
MspR9I	CCNGG	4 cut(s)	19, 230, 582, 1200
Mva1269I	GAATGC	1 cut(s)	1074
MvaI	CCWGG	3 cut(s)	230, 582, 1200
MwoI	GCNNNNNNNGC	1 cut(s)	453
NciI	CCSGG	1 cut(s)	19
NdeII	GATC	5 cut(s)	294, 375, 718, 817, 1177
NlaIII	CATG	4 cut(s)	286, 426, 564, 1001
NlaIV	GGNNCC	2 cut(s)	579, 786
NspI	RCATGY	2 cut(s)	564, 1001
NspV	TTCGAA	2 cut(s)	67, 682
PaeI	GCATGC	1 cut(s)	1001
PctI	GAATGC	1 cut(s)	1074
PfeI	GAWTC	5 cut(s)	80, 474, 643, 747, 1008
PkrI	GCNGC	3 cut(s)	220, 369, 877
PpuMI	RGGWCCY	1 cut(s)	785
PshAI	GACNNNNGTC	1 cut(s)	405
Psp1406I	AACGTT	2 cut(s)	488, 727
Psp5II	RGGWCCY	1 cut(s)	785
Psp6I	CCWGG	3 cut(s)	228, 580, 1198
PspGI	CCWGG	3 cut(s)	228, 580, 1198
PspN4I	GGNNCC	2 cut(s)	579, 786
PspPI	GGNCC	5 cut(s)	232, 253, 500, 613, 785
PspPPI	RGGWCCY	1 cut(s)	785
PstI	CTGCAG	2 cut(s)	139, 955
PstNI	CAGNNNCTG	1 cut(s)	256
RsaI	GTAC	3 cut(s)	30, 692, 1091
RsaNI	GTAC	3 cut(s)	29, 691, 1090
RseI	CAYNNNNRTG	1 cut(s)	281
SaqAI	TTAA	6 cut(s)	144, 176, 731, 839, 1083, 1225
SatI	GCNGC	3 cut(s)	219, 368, 876
Sau3AI	GATC	5 cut(s)	294, 375, 718, 817, 1177
Sau96I	GGNCC	5 cut(s)	232, 253, 500, 613, 785
ScrFI	CCNGG	4 cut(s)	19, 230, 582, 1200
SduI	GDGCHC	2 cut(s)	332, 993
SfaNI	GCATC	3 cut(s)	140, 864, 1057
SfcI	CTRYAG	2 cut(s)	135, 951
SfuI	TTCGAA	2 cut(s)	67, 682
SinI	GGWCC	2 cut(s)	500, 785
SmiMI	CAYNNNNRTG	1 cut(s)	281
SphI	GCATGC	1 cut(s)	1001
Sse9I	AATT	6 cut(s)	88, 196, 312, 551, 617, 1055
SsiI	CCGC	3 cut(s)	39, 186, 1005
SspI	AATATT	1 cut(s)	884
SspMI	CTAG	4 cut(s)	222, 309, 650, 791
StyD4I	CCNGG	4 cut(s)	17, 228, 580, 1198
TaaI	ACNGT	3 cut(s)	404, 910, 941
TaiI	ACGT	2 cut(s)	491, 730
TaqI	TCGA	7 cut(s)	67, 378, 399, 682, 750, 816, 901
TasI	AATT	6 cut(s)	88, 196, 312, 551, 617, 1055
TatI	WGTACW	2 cut(s)	28, 690
TfiI	GAWTC	5 cut(s)	80, 474, 643, 747, 1008
Tru1I	TTAA	6 cut(s)	144, 176, 731, 839, 1083, 1225
Tru9I	TTAA	6 cut(s)	144, 176, 731, 839, 1083, 1225
TscAI	CASTG	4 cut(s)	217, 484, 915, 1222
TseI	GCWGC	3 cut(s)	218, 367, 875
TspDTI	ATGAA	6 cut(s)	411, 537, 791, 981, 1047, 1119
TspGWI	ACGGA	1 cut(s)	759
TspRI	CASTG	4 cut(s)	217, 484, 915, 1222
VpaK11BI	GGWCC	2 cut(s)	500, 785
XbaI	TCTAGA	2 cut(s)	308, 790
XceI	RCATGY	2 cut(s)	564, 1001
XcmI	CCANNNNNNNNNTGG	1 cut(s)	242
XspI	CTAG	4 cut(s)	222, 309, 650, 791

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.