MD15G1227000.v1.1

Vacuolar iron transporter homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016935
Physical Location & Seq
Forward (+)
18449646 .. 18451051
1406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1227000.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGATACTTCTCGTCGAACGCTCGAAACCCCAGTTGAGCACAAGCTTCCTGTAACTGATGACGAGAAATACGCGAACACAAGGACCGAGCAAGTGCAGAGAGCTCAGTGGCTCAGAGCTGCTCTTCTGGGAGCAAGCGACGGGTTACTGTCAACCACGTCCTTGATGCTTGGCGTCGGGGCAGCGAAGGAAGATCGTGGGACGATGGTCTTATCCGGCATAGCGGGAGCTGTTGCAGGAGCTTGCAGCATGGCTGTGGGGGAGTTTGTTTCGGTATCAACACAAAGAGATATTGAAAGGACTTCTCTTTTAAGACAGGGCGGTTTCAAAACTGAGACGGGTAAGAAAGAGATGAAAGAACAATCTAACCTAACCATTTCAGTTGAACTGACTCCTTCAACAGGACTCCCATGTGGAAATTCAGCATCCAAGCCGCCGGAGCAGCCGGAGAAAACGAGGAAGATTACTGCCATTGAGATCACACAAATTAGGGAAGGGAAGGAGTCTTTGCCAAGTCCACACAAGGCAGCAGCTGCATCAGCCTTGGCATTTCTGTGTGGCTCACTTGTGCCTTTACTCTCAGCCATGTTTATTCCTCAAAATACTATAAGAATGGCGGTGGTTGTGGTAGTGGCTTCACTAGCTCTGGCTCTCTTTGGCTGCGTGGGAGCTCGTATGGGCGGCTCCCCGGCGATGCCATCTGCTGTTAGGGTTTTAGTTGGAGGTTGGATAGCAATGGCAATCACTTATGCCTTGCTCACCCCTTTTGAAAAGGAAAGTCCCAATGAAAAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

266

Amino Acids

27.99

Weight (kDa)

8.34

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 38 - 252 1e-46 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016935)

Species Orthologous Gene IDs
malus_domestica MD15G1227000.v1.1
prunus_persica Prupe.7G188300_v2.0.a1
pyrus_communis pycom02g08630
rosa_chinensis RchiOBHm_Chr2g0096671
rosa_laevigata RLG00000016660
rosa_multiflora Rmu_sc0003565.1_g000019 Rmu_sc0003565.1_g000020
rosa_roxburghii Rroxscaffold_2G00145430
rosa_rugosa Rorug02G0059500
rosa_samantha Rh2AG106900 Rh2BG109300 Rh2CG111100 Rh2DG110700
rosa_wichuraiana Rw2G008280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 74
AciI CCGC 5 cut(s) 224, 321, 434, 617, 681
AcsI RAATTY 1 cut(s) 418
AcyI GRCGYC 1 cut(s) 174
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 5 cut(s) 296, 328, 386, 399, 770
AjiI CACGTC 1 cut(s) 159
AluBI AGCT 8 cut(s) 46, 104, 119, 230, 242, 533, 644, 671
AluI AGCT 8 cut(s) 46, 104, 119, 230, 242, 533, 644, 671
Alw21I GWGCWC 3 cut(s) 42, 106, 673
Alw26I GTCTC 1 cut(s) 329
AlwNI CAGNNNCTG 1 cut(s) 533
ApeKI GCWGC 8 cut(s) 119, 182, 246, 442, 527, 530, 533, 660
ApoI RAATTY 1 cut(s) 418
AspS9I GGNCC 1 cut(s) 84
AsuC2I CCSGG 1 cut(s) 689
AsuHPI GGTGA 1 cut(s) 751
AvaII GGWCC 1 cut(s) 84
BanII GRGCYC 2 cut(s) 106, 673
Bbv12I GWGCWC 3 cut(s) 42, 106, 673
BbvI GCAGC 8 cut(s) 106, 194, 258, 454, 520, 539, 542, 647
BccI CCATC 2 cut(s) 199, 706
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 1 cut(s) 641
Bme1390I CCNGG 1 cut(s) 689
Bme18I GGWCC 1 cut(s) 84
BmgBI CACGTC 1 cut(s) 159
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 685
BmrFI CCNGG 1 cut(s) 689
BmrI ACTGGG 1 cut(s) 26
BmsI GCATC 4 cut(s) 156, 434, 545, 684
BmuI ACTGGG 1 cut(s) 26
BoxI GACNNNNGTC 1 cut(s) 206
BpuMI CCSGG 1 cut(s) 689
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 543, 687
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse1I ACTGG 1 cut(s) 32
Bse3DI GCAATG 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 543, 687
BseGI GGATG 1 cut(s) 425
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 4 cut(s) 119, 127, 324, 594
BseNI ACTGG 1 cut(s) 32
BseXI GCAGC 8 cut(s) 106, 194, 258, 454, 520, 539, 542, 647
BsgI GTGCAG 1 cut(s) 116
Bsh1236I CGCG 1 cut(s) 74
BsiHKAI GWGCWC 3 cut(s) 42, 106, 673
BsiSI CCGG 4 cut(s) 216, 437, 446, 689
BslFI GGGAC 2 cut(s) 214, 765
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 329
BsmBI CGTCTC 1 cut(s) 329
BsmFI GGGAC 2 cut(s) 214, 765
Bsp1286I GDGCHC 3 cut(s) 42, 106, 673
Bsp143I GATC 2 cut(s) 193, 477
BspACI CCGC 5 cut(s) 224, 321, 434, 617, 681
BspCNI CTCAG 4 cut(s) 118, 126, 325, 593
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 685
BspQI GCTCTTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 741
BsrI ACTGG 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 543, 687
BssMI GATC 2 cut(s) 193, 477
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 543
Bst4CI ACNGT 1 cut(s) 150
Bst6I CTCTTC 1 cut(s) 129
BstACI GRCGYC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 533
BstC8I GCNNGC 2 cut(s) 136, 244
BstDEI CTNAG 4 cut(s) 105, 113, 333, 580
BstENI CCTNNNNNAGG 1 cut(s) 399
BstF5I GGATG 1 cut(s) 425
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 2 cut(s) 196, 480
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 2 cut(s) 193, 477
BstMWI GCNNNNNNNGC 5 cut(s) 439, 442, 533, 539, 641
BstPAI GACNNNNGTC 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 687
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 8 cut(s) 106, 194, 258, 454, 520, 539, 542, 647
BtgZI GCGATG 1 cut(s) 707
BtrI CACGTC 1 cut(s) 159
BtsCI GGATG 1 cut(s) 425
BtsIMutI CAGTG 1 cut(s) 113
Cac8I GCNNGC 2 cut(s) 136, 244
CaiI CAGNNNCTG 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 84
CseI GACGC 1 cut(s) 163
CviAII CATG 3 cut(s) 250, 411, 586
DdeI CTNAG 4 cut(s) 105, 113, 333, 580
DpnI GATC 2 cut(s) 195, 479
DpnII GATC 2 cut(s) 193, 477
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Ecl136II GAGCTC 2 cut(s) 104, 671
Eco130I CCWWGG 1 cut(s) 543
Eco24I GRGCYC 2 cut(s) 106, 673
Eco47I GGWCC 1 cut(s) 84
Eco53kI GAGCTC 2 cut(s) 104, 671
EcoICRI GAGCTC 2 cut(s) 104, 671
EcoNI CCTNNNNNAGG 1 cut(s) 399
EcoT14I CCWWGG 1 cut(s) 543
EcoT38I GRGCYC 2 cut(s) 106, 673
ErhI CCWWGG 1 cut(s) 543
Esp3I CGTCTC 1 cut(s) 329
FaeI CATG 3 cut(s) 253, 414, 589
FaiI YATR 7 cut(s) 221, 251, 412, 587, 608, 677, 750
FaqI GGGAC 2 cut(s) 214, 765
FatI CATG 3 cut(s) 249, 410, 585
FauI CCCGC 1 cut(s) 217
FokI GGATG 1 cut(s) 412
FriOI GRGCYC 2 cut(s) 106, 673
FspBI CTAG 1 cut(s) 641
HapII CCGG 4 cut(s) 216, 437, 446, 689
HgaI GACGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 3 cut(s) 253, 414, 589
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 3 cut(s) 391, 405, 503
HpaII CCGG 4 cut(s) 216, 437, 446, 689
HphI GGTGA 1 cut(s) 751
Hpy166II GTNNAC 2 cut(s) 153, 518
Hpy188I TCNGA 1 cut(s) 116
Hpy8I GTNNAC 2 cut(s) 153, 518
Hpy99I CGWCG 3 cut(s) 18, 143, 179
HpyAV CCTTC 4 cut(s) 181, 405, 488, 493
HpyCH4III ACNGT 1 cut(s) 150
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 4 cut(s) 97, 236, 246, 536
HpyF10VI GCNNNNNNNGC 5 cut(s) 439, 442, 533, 539, 641
HpyF3I CTNAG 4 cut(s) 105, 113, 333, 580
HpySE526I ACGT 1 cut(s) 158
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 3 cut(s) 253, 414, 589
Kzo9I GATC 2 cut(s) 193, 477
LguI GCTCTTC 1 cut(s) 129
LmnI GCTCC 6 cut(s) 131, 227, 239, 439, 668, 689
Lsp1109I GCAGC 8 cut(s) 106, 194, 258, 454, 520, 539, 542, 647
LweI GCATC 4 cut(s) 156, 434, 545, 684
MaeI CTAG 1 cut(s) 641
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 2 cut(s) 52, 144
MalI GATC 2 cut(s) 195, 479
MboI GATC 2 cut(s) 193, 477
MboII GAAGA 3 cut(s) 116, 203, 472
MhlI GDGCHC 3 cut(s) 42, 106, 673
MluCI AATT 2 cut(s) 418, 486
MlyI GAGTC 3 cut(s) 385, 399, 512
MmeI TCCRAC 2 cut(s) 700, 707
MnlI CCTC 3 cut(s) 450, 606, 716
MseI TTAA 1 cut(s) 311
MslI CAYNNNNRTG 2 cut(s) 254, 553
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 4 cut(s) 216, 437, 446, 689
MspR9I CCNGG 1 cut(s) 689
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 5 cut(s) 439, 442, 533, 539, 641
NciI CCSGG 1 cut(s) 689
NdeII GATC 2 cut(s) 193, 477
NlaIII CATG 3 cut(s) 253, 414, 589
NlaIV GGNNCC 1 cut(s) 685
PciSI GCTCTTC 1 cut(s) 129
PleI GAGTC 3 cut(s) 385, 399, 511
PpsI GAGTC 3 cut(s) 385, 399, 511
PshAI GACNNNNGTC 1 cut(s) 206
Psp124BI GAGCTC 2 cut(s) 106, 673
PspN4I GGNNCC 1 cut(s) 685
PspPI GGNCC 1 cut(s) 84
PstNI CAGNNNCTG 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 533
RseI CAYNNNNRTG 2 cut(s) 254, 553
SacI GAGCTC 2 cut(s) 106, 673
SapI GCTCTTC 1 cut(s) 129
SaqAI TTAA 1 cut(s) 311
Sau3AI GATC 2 cut(s) 193, 477
Sau96I GGNCC 1 cut(s) 84
SchI GAGTC 3 cut(s) 385, 399, 512
ScrFI CCNGG 1 cut(s) 689
SduI GDGCHC 3 cut(s) 42, 106, 673
SfaNI GCATC 4 cut(s) 156, 434, 545, 684
SinI GGWCC 1 cut(s) 84
SmiMI CAYNNNNRTG 2 cut(s) 254, 553
Sse9I AATT 2 cut(s) 418, 486
SsiI CCGC 5 cut(s) 224, 321, 434, 617, 681
SspMI CTAG 1 cut(s) 641
SstI GAGCTC 2 cut(s) 106, 673
StyD4I CCNGG 1 cut(s) 687
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 1 cut(s) 150
TaiI ACGT 1 cut(s) 161
TaqI TCGA 2 cut(s) 16, 24
TaqII GACCGA 1 cut(s) 101
TasI AATT 2 cut(s) 418, 486
TauI GCSGC 2 cut(s) 436, 684
Tru1I TTAA 1 cut(s) 311
Tru9I TTAA 1 cut(s) 311
TscAI CASTG 1 cut(s) 113
TseI GCWGC 8 cut(s) 119, 182, 246, 442, 527, 530, 533, 660
TspDTI ATGAA 1 cut(s) 368
TspRI CASTG 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 84
XagI CCTNNNNNAGG 1 cut(s) 399
XapI RAATTY 1 cut(s) 418
XspI CTAG 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.