Rorug02G0059500

Vacuolar iron transporter homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0016935
Physical Location & Seq
Reverse (-)
4579525 .. 4581162
1638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0059500.1

Sequence Viewer

Length: 1488 bp
ATGGAGGATGAGAGTGGACTTGAGCTTAGCTTGGGTCTATCTTGTGGTGGGGGATCTTCTGTGAAGTCCAAGGGTAAAAATGGTGCCTCCTCAAATACTAGAGCGGAAGAAGGTGATAGAGACAACAAACTAGCGGATGATTTTAAAGACTTTCTTCATGGAGGGGCTCAGAAGCAAGAGTCAAGTAGTAGCTCTCAAAGAAGTGATTCAGTGAAACCTAAGGAAAACTTCTTTAATGACCTCTCCAAGGCCAATGGTGATGCAGACGCATCAATGAACTTGAATGGGAGAGGAGTCTGGGCTGCAAAGAATAACAAGTCTGATGAAATAGAGGAAAGACAAACAGATGCTGGTAATAAACGCAAGACTTTGTTTGAAGAGATGAACCAACAGAAGAAGCATGAGAGAGAAGCTCATTACACTGATATGCATGACAAGACGAGAGCATCCCATATTTCCATAACAGAAGATGGCTCTACTGCTGAAAATGAAGACGTGGCAGATTCTGAAGTAGAGGGCTCAGCCTCAAGGCTGATATCCCAACATGACGATGGCTCTAAACGATTTGTTGGATCTGGTGACTCATCTGATGCTCCAAAAGAGCTTCGCAGGTTTGCTGATTCAAGTAATGTGGATCCAAATGGGCAGAAGAGGTTTAATATTTCATCAGAAAATGATTACAAGGTTGGGAACATGACGTATGGCAGTCCATTTTCTGTGCAATCAGTAAACATGATGAATATGCCCTACTCTCTCCCGAAGGATTCTAACCCTGTTGGTCCACCCAGCACATCAGGCCATCTGATTCCTGCAATGATGCAGGTGATGCCTATTGCAAGTGAACGGTCAGGGACTCAACCCATGAATCCTGGAAACTTGCCAGTTATGTTTGGCTATTCACCTGTCCAGCTTCCAATATTGGATAAGGATAATTCTTGGGGTCCCCATACTCAGCAGTTTCACTATGCTGGCAGAAATCCACCAAACCCAGCTGTGATGCAAGTAATTTCGCATAATACATCTGAGGCTGCACAGTATGATGGGAGATTAGAACGGGCAAAAGGTGATGGAAAACAGCATGTCACTGAAGAGGGCTCCTCTTCTCAAGCAGAAGAAGACATGAAAGTAAGCAGCACGAACCCAAGGACAAAAGATGCACCTGATCGCTCAATGGCAGAGGATTTCTCTCTTGATTTTTCAGCTATAAAACCAGGAATTGCTGCGGACATAAAATTTGGGGGATCTGGTTCCCGCCCAAATCTACCATGGGTTTCTACCAAAGGTCCAGGCCCCAATGGTAGGACGATTTCTGGTGTCACTTACAGATACAGCACAAACCAAATCAAAATAGTTTGTGCTTGCCACGGTTCCCACATGTCCCCTGATGATTTTGTTCGGCATGCAAGTGAAGAGCCTTCTGATCCAGAAAGCGGCACAGGTTTGGCGACAGTCCCAAATGGCAATCCTGCTGCCTCTGCTCAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

495

Amino Acids

53.33

Weight (kDa)

5.33

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016935)

Species Orthologous Gene IDs
malus_domestica MD15G1227000.v1.1
prunus_persica Prupe.7G188300_v2.0.a1
pyrus_communis pycom02g08630
rosa_chinensis RchiOBHm_Chr2g0096671
rosa_laevigata RLG00000016660
rosa_multiflora Rmu_sc0003565.1_g000019 Rmu_sc0003565.1_g000020
rosa_roxburghii Rroxscaffold_2G00145430
rosa_rugosa Rorug02G0059500
rosa_samantha Rh2AG106900 Rh2BG109300 Rh2CG111100 Rh2DG110700
rosa_wichuraiana Rw2G008280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 811
Acc36I ACCTGC 2 cut(s) 600, 811
AccB1I GGYRCC 1 cut(s) 83
AccBSI CCGCTC 1 cut(s) 104
AciI CCGC 5 cut(s) 104, 134, 1223, 1252, 1431
AclWI GGATC 6 cut(s) 61, 580, 629, 642, 1249, 1415
AcsI RAATTY 1 cut(s) 1232
AcuI CTGAAG 2 cut(s) 528, 1107
AfiI CCNNNNNNNGG 3 cut(s) 247, 1299, 1430
AflIII ACRYGT 1 cut(s) 1374
AgsI TTSAA 3 cut(s) 283, 377, 624
AjiI CACGTC 1 cut(s) 496
AjnI CCWGG 3 cut(s) 868, 1210, 1285
AluBI AGCT 9 cut(s) 25, 30, 192, 413, 604, 910, 992, 1202, 1485
AluI AGCT 9 cut(s) 25, 30, 192, 413, 604, 910, 992, 1202, 1485
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 6 cut(s) 61, 580, 629, 642, 1249, 1415
AlwNI CAGNNNCTG 2 cut(s) 350, 506
AoxI GGCC 3 cut(s) 249, 796, 1288
ApeKI GCWGC 5 cut(s) 302, 1028, 1131, 1220, 1469
ApoI RAATTY 1 cut(s) 1232
ArsI GACNNNNNNTTYG 2 cut(s) 1217, 1249
Asp700I GAANNNNTTC 1 cut(s) 205
AspS9I GGNCC 4 cut(s) 779, 941, 1283, 1289
AsuHPI GGTGA 6 cut(s) 125, 269, 590, 835, 891, 1076
AvaII GGWCC 3 cut(s) 779, 941, 1283
AxyI CCTNAGG 1 cut(s) 219
BamHI GGATCC 1 cut(s) 634
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 3 cut(s) 169, 521, 1097
BbsI GAAGAC 2 cut(s) 498, 1122
BbvI GCAGC 5 cut(s) 289, 1015, 1143, 1207, 1456
BccI CCATC 5 cut(s) 464, 545, 807, 1034, 1061
BciT130I CCWGG 3 cut(s) 870, 1212, 1287
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 2 cut(s) 99, 131
BfuAI ACCTGC 2 cut(s) 600, 811
BisI GCNGC 6 cut(s) 303, 1029, 1132, 1221, 1432, 1470
BlpI GCTNAGC 2 cut(s) 26, 520
BlsI GCNGC 6 cut(s) 304, 1030, 1133, 1222, 1433, 1471
Bme1390I CCNGG 3 cut(s) 870, 1212, 1287
Bme18I GGWCC 3 cut(s) 779, 941, 1283
BmgBI CACGTC 1 cut(s) 496
BmgT120I GGNCC 4 cut(s) 779, 941, 1283, 1289
BmiI GGNNCC 8 cut(s) 85, 636, 942, 943, 1096, 1249, 1291, 1369
BmrFI CCNGG 3 cut(s) 870, 1212, 1287
BmsI GCATC 9 cut(s) 250, 278, 337, 455, 580, 807, 816, 987, 1144
BpiI GAAGAC 2 cut(s) 498, 1122
BplI GAGNNNNNCTC 6 cut(s) 397, 429, 1082, 1114, 1169, 1201
Bpu1102I GCTNAGC 2 cut(s) 26, 520
BpuEI CTTGAG 3 cut(s) 41, 511, 1089
BsaJI CCNNGG 5 cut(s) 69, 246, 1142, 1265, 1363
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 1299, 1430
Bse1I ACTGG 1 cut(s) 881
Bse21I CCTNAGG 1 cut(s) 219
Bse3DI GCAATG 1 cut(s) 819
BseBI CCWGG 3 cut(s) 870, 1212, 1287
BseDI CCNNGG 5 cut(s) 69, 246, 1142, 1265, 1363
BseGI GGATG 3 cut(s) 13, 142, 446
BseLI CCNNNNNNNGG 3 cut(s) 247, 1299, 1430
BseMI GCAATG 1 cut(s) 819
BseMII CTCAG 4 cut(s) 182, 534, 965, 1014
BseNI ACTGG 1 cut(s) 881
BseRI GAGGAG 3 cut(s) 79, 306, 1087
BseXI GCAGC 5 cut(s) 289, 1015, 1143, 1207, 1456
BseYI CCCAGC 2 cut(s) 785, 988
BsgI GTGCAG 1 cut(s) 1014
BshFI GGCC 3 cut(s) 251, 798, 1290
BshNI GGYRCC 1 cut(s) 83
BslFI GGGAC 4 cut(s) 865, 927, 1363, 1436
BslI CCNNNNNNNGG 3 cut(s) 247, 1299, 1430
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 4 cut(s) 865, 927, 1363, 1436
BsnI GGCC 3 cut(s) 251, 798, 1290
Bsp1286I GDGCHC 3 cut(s) 169, 521, 1097
Bsp143I GATC 6 cut(s) 53, 572, 634, 1162, 1241, 1420
Bsp1720I GCTNAGC 2 cut(s) 26, 520
Bsp19I CCATGG 1 cut(s) 1265
BspACI CCGC 5 cut(s) 104, 134, 1223, 1252, 1431
BspANI GGCC 3 cut(s) 251, 798, 1290
BspCNI CTCAG 4 cut(s) 181, 533, 964, 1015
BspLI GGNNCC 8 cut(s) 85, 636, 942, 943, 1096, 1249, 1291, 1369
BspMI ACCTGC 2 cut(s) 600, 811
BspPI GGATC 6 cut(s) 61, 580, 629, 642, 1249, 1415
BspQI GCTCTTC 1 cut(s) 1404
BspT107I GGYRCC 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 819
BsrI ACTGG 1 cut(s) 881
BssECI CCNNGG 5 cut(s) 69, 246, 1142, 1265, 1363
BssMI GATC 6 cut(s) 53, 572, 634, 1162, 1241, 1420
BssT1I CCWWGG 4 cut(s) 69, 246, 1142, 1265
Bst2UI CCWGG 3 cut(s) 870, 1212, 1287
Bst4CI ACNGT 4 cut(s) 846, 1035, 1367, 1450
Bst6I CTCTTC 5 cut(s) 372, 644, 1083, 1105, 1404
BstAPI GCANNNNNTGC 1 cut(s) 826
BstC8I GCNNGC 3 cut(s) 970, 1360, 1401
BstDEI CTNAG 7 cut(s) 26, 168, 219, 520, 951, 1023, 1479
BstDSI CCRYGG 2 cut(s) 1265, 1363
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 3 cut(s) 13, 142, 446
BstKTI GATC 6 cut(s) 56, 575, 637, 1165, 1244, 1423
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 6 cut(s) 53, 572, 634, 1162, 1241, 1420
BstMWI GCNNNNNNNGC 3 cut(s) 795, 826, 1475
BstNI CCWGG 3 cut(s) 870, 1212, 1287
BstNSI RCATGY 3 cut(s) 1082, 1378, 1403
BstSCI CCNGG 3 cut(s) 868, 1210, 1285
BstV1I GCAGC 5 cut(s) 289, 1015, 1143, 1207, 1456
BstV2I GAAGAC 2 cut(s) 498, 1122
BstX2I RGATCY 4 cut(s) 53, 572, 634, 1241
BstYI RGATCY 4 cut(s) 53, 572, 634, 1241
Bsu36I CCTNAGG 1 cut(s) 219
BsuRI GGCC 3 cut(s) 251, 798, 1290
BtgI CCRYGG 2 cut(s) 1265, 1363
BtrI CACGTC 1 cut(s) 496
BtsCI GGATG 3 cut(s) 13, 142, 446
BtsIMutI CAGTG 3 cut(s) 216, 420, 1083
BveI ACCTGC 2 cut(s) 600, 811
Cac8I GCNNGC 3 cut(s) 970, 1360, 1401
CaiI CAGNNNCTG 2 cut(s) 350, 506
Cfr13I GGNCC 4 cut(s) 779, 941, 1283, 1289
CseI GACGC 1 cut(s) 275
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
DdeI CTNAG 7 cut(s) 26, 168, 219, 520, 951, 1023, 1479
DpnI GATC 6 cut(s) 55, 574, 636, 1164, 1243, 1422
DpnII GATC 6 cut(s) 53, 572, 634, 1162, 1241, 1420
DraI TTTAAA 1 cut(s) 145
Eam1104I CTCTTC 5 cut(s) 372, 644, 1083, 1105, 1404
EarI CTCTTC 5 cut(s) 372, 644, 1083, 1105, 1404
Eco130I CCWWGG 4 cut(s) 69, 246, 1142, 1265
Eco24I GRGCYC 3 cut(s) 169, 521, 1097
Eco32I GATATC 1 cut(s) 537
Eco47I GGWCC 3 cut(s) 779, 941, 1283
Eco57I CTGAAG 2 cut(s) 528, 1107
Eco81I CCTNAGG 1 cut(s) 219
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoO109I RGGNCCY 2 cut(s) 941, 1289
EcoRII CCWGG 3 cut(s) 868, 1210, 1285
EcoRV GATATC 1 cut(s) 537
EcoT14I CCWWGG 4 cut(s) 69, 246, 1142, 1265
EcoT22I ATGCAT 1 cut(s) 432
EcoT38I GRGCYC 3 cut(s) 169, 521, 1097
ErhI CCWWGG 4 cut(s) 69, 246, 1142, 1265
FalI AAGNNNNNCTT 4 cut(s) 138, 170, 212, 244
FaqI GGGAC 4 cut(s) 865, 927, 1363, 1436
FauI CCCGC 1 cut(s) 1259
Fnu4HI GCNGC 6 cut(s) 303, 1029, 1132, 1221, 1432, 1470
FokI GGATG 3 cut(s) 20, 149, 433
FriOI GRGCYC 3 cut(s) 169, 521, 1097
Fsp4HI GCNGC 6 cut(s) 303, 1029, 1132, 1221, 1432, 1470
FspBI CTAG 2 cut(s) 99, 131
GluI GCNGC 6 cut(s) 303, 1029, 1132, 1221, 1432, 1470
GsaI CCCAGC 2 cut(s) 789, 992
HaeIII GGCC 3 cut(s) 251, 798, 1290
HgaI GACGC 1 cut(s) 275
HphI GGTGA 6 cut(s) 125, 269, 590, 835, 891, 1076
Hpy166II GTNNAC 4 cut(s) 17, 730, 782, 842
Hpy188I TCNGA 9 cut(s) 171, 322, 508, 589, 670, 804, 1024, 1420, 1482
Hpy188III TCNNGA 3 cut(s) 757, 1190, 1424
Hpy8I GTNNAC 4 cut(s) 17, 730, 782, 842
HpyAV CCTTC 3 cut(s) 104, 754, 1425
HpyCH4III ACNGT 4 cut(s) 846, 1035, 1367, 1450
HpyCH4IV ACGT 2 cut(s) 495, 698
HpyF10VI GCNNNNNNNGC 3 cut(s) 795, 826, 1475
HpyF3I CTNAG 7 cut(s) 26, 168, 219, 520, 951, 1023, 1479
HpySE526I ACGT 2 cut(s) 495, 698
KflI GGGWCCC 1 cut(s) 941
Kzo9I GATC 6 cut(s) 53, 572, 634, 1162, 1241, 1420
LguI GCTCTTC 1 cut(s) 1404
LmnI GCTCC 2 cut(s) 598, 1100
Lsp1109I GCAGC 5 cut(s) 289, 1015, 1143, 1207, 1456
LweI GCATC 9 cut(s) 250, 278, 337, 455, 580, 807, 816, 987, 1144
MaeI CTAG 2 cut(s) 99, 131
MaeII ACGT 2 cut(s) 495, 698
MaeIII GTNAC 3 cut(s) 578, 1081, 1315
MalI GATC 6 cut(s) 55, 574, 636, 1164, 1243, 1422
MbiI CCGCTC 1 cut(s) 104
MboI GATC 6 cut(s) 53, 572, 634, 1162, 1241, 1420
MflI RGATCY 4 cut(s) 53, 572, 634, 1241
MhlI GDGCHC 3 cut(s) 169, 521, 1097
MluCI AATT 4 cut(s) 931, 1005, 1215, 1232
MlyI GAGTC 4 cut(s) 188, 303, 575, 847
MmeI TCCRAC 1 cut(s) 550
Mph1103I ATGCAT 1 cut(s) 432
MroXI GAANNNNTTC 1 cut(s) 205
MseI TTAA 3 cut(s) 144, 234, 657
MslI CAYNNNNRTG 3 cut(s) 425, 549, 1404
MspA1I CMGCKG 1 cut(s) 992
MspR9I CCNGG 3 cut(s) 870, 1212, 1287
MvaI CCWGG 3 cut(s) 870, 1212, 1287
MwoI GCNNNNNNNGC 3 cut(s) 795, 826, 1475
NcoI CCATGG 1 cut(s) 1265
NdeII GATC 6 cut(s) 53, 572, 634, 1162, 1241, 1420
NlaIV GGNNCC 8 cut(s) 85, 636, 942, 943, 1096, 1249, 1291, 1369
NmuCI GTSAC 3 cut(s) 578, 1081, 1315
NsiI ATGCAT 1 cut(s) 432
NspI RCATGY 3 cut(s) 1082, 1378, 1403
PaeI GCATGC 1 cut(s) 1403
PaqCI CACCTGC 1 cut(s) 811
PciI ACATGT 1 cut(s) 1374
PciSI GCTCTTC 1 cut(s) 1404
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 6 cut(s) 206, 503, 620, 764, 805, 865
PfoI TCCNGGA 1 cut(s) 868
PkrI GCNGC 6 cut(s) 304, 1030, 1133, 1222, 1433, 1471
PleI GAGTC 4 cut(s) 187, 302, 575, 847
PpsI GAGTC 4 cut(s) 187, 302, 575, 847
PpuMI RGGWCCY 1 cut(s) 941
PscI ACATGT 1 cut(s) 1374
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 3 cut(s) 868, 1210, 1285
PspFI CCCAGC 2 cut(s) 785, 988
PspGI CCWGG 3 cut(s) 868, 1210, 1285
PspN4I GGNNCC 8 cut(s) 85, 636, 942, 943, 1096, 1249, 1291, 1369
PspPI GGNCC 4 cut(s) 779, 941, 1283, 1289
PspPPI RGGWCCY 1 cut(s) 941
PstNI CAGNNNCTG 2 cut(s) 350, 506
PsuI RGATCY 4 cut(s) 53, 572, 634, 1241
PvuII CAGCTG 1 cut(s) 992
RseI CAYNNNNRTG 3 cut(s) 425, 549, 1404
SapI GCTCTTC 1 cut(s) 1404
SaqAI TTAA 3 cut(s) 144, 234, 657
SatI GCNGC 6 cut(s) 303, 1029, 1132, 1221, 1432, 1470
Sau3AI GATC 6 cut(s) 53, 572, 634, 1162, 1241, 1420
Sau96I GGNCC 4 cut(s) 779, 941, 1283, 1289
SchI GAGTC 4 cut(s) 188, 303, 575, 847
ScrFI CCNGG 3 cut(s) 870, 1212, 1287
SduI GDGCHC 3 cut(s) 169, 521, 1097
SfaNI GCATC 9 cut(s) 250, 278, 337, 455, 580, 807, 816, 987, 1144
SinI GGWCC 3 cut(s) 779, 941, 1283
SmiMI CAYNNNNRTG 3 cut(s) 425, 549, 1404
SmlI CTYRAG 3 cut(s) 20, 526, 1104
SmoI CTYRAG 3 cut(s) 20, 526, 1104
SphI GCATGC 1 cut(s) 1403
Sse9I AATT 4 cut(s) 931, 1005, 1215, 1232
SsiI CCGC 5 cut(s) 104, 134, 1223, 1252, 1431
SspI AATATT 2 cut(s) 661, 918
SspMI CTAG 2 cut(s) 99, 131
StyD4I CCNGG 3 cut(s) 868, 1210, 1285
StyI CCWWGG 4 cut(s) 69, 246, 1142, 1265
TaaI ACNGT 4 cut(s) 846, 1035, 1367, 1450
TaiI ACGT 2 cut(s) 498, 701
TasI AATT 4 cut(s) 931, 1005, 1215, 1232
TauI GCSGC 1 cut(s) 1434
TfiI GAWTC 6 cut(s) 206, 503, 620, 764, 805, 865
Tru1I TTAA 3 cut(s) 144, 234, 657
Tru9I TTAA 3 cut(s) 144, 234, 657
TscAI CASTG 3 cut(s) 216, 427, 1090
TseFI GTSAC 3 cut(s) 578, 1081, 1315
TseI GCWGC 5 cut(s) 302, 1028, 1131, 1220, 1469
Tsp45I GTSAC 3 cut(s) 578, 1081, 1315
TspDTI ATGAA 9 cut(s) 146, 290, 339, 398, 504, 654, 752, 878, 1136
TspRI CASTG 3 cut(s) 216, 427, 1090
VpaK11BI GGWCC 3 cut(s) 779, 941, 1283
XagI CCTNNNNNAGG 1 cut(s) 245
XapI RAATTY 1 cut(s) 1232
XceI RCATGY 3 cut(s) 1082, 1378, 1403
XcmI CCANNNNNNNNNTGG 2 cut(s) 548, 1263
XmnI GAANNNNTTC 1 cut(s) 205
XspI CTAG 2 cut(s) 99, 131
Zsp2I ATGCAT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.