MD15G1249900.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016729
Physical Location & Seq
Reverse (-)
20896132 .. 20897405
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1249900.v1.1.491

Sequence Viewer

Length: 822 bp
ATGAAGATCTCAGTTCACAACAAAACCCTAGTCCTCTTTCTCTCACAACTCCTCTCACTCCTTGTCTCCAAAGCATGCCATTCAGTAGACAAACAAGCACTTCTCCATTTCAAGCACAACATCATTTCGGACCCTTCTAAATTGCTCCATTCATGGTCACTCTCCTCCGACTGCTGCTCCGCTTGGGAAGGCATCGCGTGTGACCCTTCTGGCAGAGTTAGCTTGATATATCTTGATCTATCCGGAAATCGGATAAGTGGAAGACTACCTCAGTCCATTGTTGCAGTTTCGCAATTAGTCCTGCTGTATCTCAGTCATAATCAGCTCAATGGAAGCATTCCGTGTTCGATTTCTGGGCTTAATTCTCTGCTGTTTTGTCAGTTATCCCAAAACAAGCTCGGAGGTGCTTTGCCAGCATCACTAGGCCAGCTCCCAAAGATAGAAAGGCTCATTTTCGAGAACAACAAACTTTCAGGAAAATTACCAGCAATCATTGGCCACCTTGCAATTCTCACTGACATTTTCTTCTCCAACAATCGTTTTACAGGCAAGATTCCTTCAAGTTTTTCCAATTTACATAACCTACAAACAGTAGATTTGTCGACAAATCGATTCATTGGTCAAATTCCACCTCAGCTAGCAAAATTACAAAGACTAGACACTCTAGACCTTTCATTTAATCCTTTGGGATTGATTAGTGTACCAAGCTTTTTTGCAAGATTGAAACTTTTTCGGCTCTCGTTGGAAAAAACCGGCATTGAAGGGCAGCTTCCGTATCTTCATCATCTGTCTCCATATTTGACTTATCAAGCAATTCCTTGA

Protein Analysis

274

Amino Acids

30.05

Weight (kDa)

9.39

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 9.9e-10 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016729)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12240
malus_domestica MD15G1249900.v1.1
prunus_persica Prupe.7G164400_v2.0.a1
pyrus_communis pycom02g10760 pycom15g22020
rosa_chinensis RchiOBHm_Chr2g0100021
rosa_laevigata RLG00000016930
rosa_roxburghii Rroxscaffold_2G00142630
rosa_rugosa Rorug02G0084700
rosa_samantha Rh2AG133800 Rh2BG137500 Rh2CG138900 Rh2DG139200
rosa_wichuraiana Rw2G010430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 87, 602
AccII CGCG 1 cut(s) 197
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 1 cut(s) 180
AcoI YGGCCR 1 cut(s) 496
AcsI RAATTY 1 cut(s) 624
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 1 cut(s) 249
AgsI TTSAA 4 cut(s) 112, 561, 724, 761
AluBI AGCT 7 cut(s) 222, 325, 397, 430, 637, 708, 769
AluI AGCT 7 cut(s) 222, 325, 397, 430, 637, 708, 769
Alw26I GTCTC 2 cut(s) 70, 795
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 2 cut(s) 424, 496
ApeKI GCWGC 2 cut(s) 174, 766
ApoI RAATTY 1 cut(s) 624
ArsI GACNNNNNNTTYG 2 cut(s) 15, 47
AspS9I GGNCC 1 cut(s) 130
AsuNHI GCTAGC 1 cut(s) 637
AvaII GGWCC 1 cut(s) 130
BalI TGGCCA 1 cut(s) 498
BbsI GAAGAC 1 cut(s) 268
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 2 cut(s) 161, 778
BcoDI GTCTC 2 cut(s) 70, 795
BfaI CTAG 5 cut(s) 29, 422, 638, 656, 665
BglII AGATCT 1 cut(s) 6
BisI GCNGC 2 cut(s) 175, 767
BlsI GCNGC 2 cut(s) 176, 768
Bme18I GGWCC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 132
BmsI GCATC 2 cut(s) 201, 425
BmtI GCTAGC 1 cut(s) 641
BpiI GAAGAC 1 cut(s) 268
Bpu10I CCTNAGC 1 cut(s) 633
Bsa29I ATCGAT 1 cut(s) 610
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 249
Bse118I RCCGGY 1 cut(s) 752
BseAI TCCGGA 1 cut(s) 242
BseCI ATCGAT 1 cut(s) 610
BseLI CCNNNNNNNGG 1 cut(s) 249
BseMII CTCAG 4 cut(s) 24, 284, 325, 647
BseRI GAGGAG 2 cut(s) 41, 154
BseXI GCAGC 2 cut(s) 161, 778
Bsh1236I CGCG 1 cut(s) 197
BshFI GGCC 2 cut(s) 426, 498
BshVI ATCGAT 1 cut(s) 610
BsiSI CCGG 2 cut(s) 243, 753
BslI CCNNNNNNNGG 1 cut(s) 249
BsmAI GTCTC 2 cut(s) 70, 795
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 2 cut(s) 426, 498
Bsp13I TCCGGA 1 cut(s) 242
Bsp143I GATC 2 cut(s) 6, 235
BspACI CCGC 1 cut(s) 180
BspANI GGCC 2 cut(s) 426, 498
BspCNI CTCAG 4 cut(s) 23, 283, 324, 646
BspDI ATCGAT 1 cut(s) 610
BspEI TCCGGA 1 cut(s) 242
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 1 cut(s) 132
BspOI GCTAGC 1 cut(s) 641
BsrFI RCCGGY 1 cut(s) 752
BssAI RCCGGY 1 cut(s) 752
BssMI GATC 2 cut(s) 6, 235
Bst4CI ACNGT 1 cut(s) 592
BstC8I GCNNGC 4 cut(s) 76, 414, 428, 639
BstDEI CTNAG 4 cut(s) 10, 270, 311, 633
BstFNI CGCG 1 cut(s) 197
BstKTI GATC 2 cut(s) 9, 238
BstMAI GTCTC 2 cut(s) 70, 795
BstMBI GATC 2 cut(s) 6, 235
BstMWI GCNNNNNNNGC 2 cut(s) 219, 413
BstNSI RCATGY 1 cut(s) 78
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 161, 778
BstV2I GAAGAC 1 cut(s) 268
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
Bsu15I ATCGAT 1 cut(s) 610
BsuRI GGCC 2 cut(s) 426, 498
BsuTUI ATCGAT 1 cut(s) 610
BtgZI GCGATG 1 cut(s) 178
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 4 cut(s) 76, 414, 428, 639
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 1 cut(s) 130
ClaI ATCGAT 1 cut(s) 610
Csp6I GTAC 1 cut(s) 701
CviAII CATG 2 cut(s) 75, 153
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 4 cut(s) 10, 270, 311, 633
DpnI GATC 2 cut(s) 8, 237
DpnII GATC 2 cut(s) 6, 235
EaeI YGGCCR 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 130
FaeI CATG 2 cut(s) 78, 156
FaiI YATR 6 cut(s) 76, 154, 229, 318, 579, 796
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FatI CATG 2 cut(s) 74, 152
FblI GTMKAC 2 cut(s) 87, 602
Fnu4HI GCNGC 2 cut(s) 175, 767
Fsp4HI GCNGC 2 cut(s) 175, 767
FspBI CTAG 5 cut(s) 29, 422, 638, 656, 665
GluI GCNGC 2 cut(s) 175, 767
HaeIII GGCC 2 cut(s) 426, 498
HapII CCGG 2 cut(s) 243, 753
Hin1II CATG 2 cut(s) 78, 156
HincII GTYRAC 1 cut(s) 603
HindII GTYRAC 1 cut(s) 603
HindIII AAGCTT 1 cut(s) 706
HinfI GANTC 2 cut(s) 553, 612
HpaII CCGG 2 cut(s) 243, 753
Hpy166II GTNNAC 4 cut(s) 16, 88, 603, 701
Hpy188I TCNGA 4 cut(s) 130, 169, 252, 401
Hpy188III TCNNGA 5 cut(s) 233, 243, 457, 474, 665
Hpy8I GTNNAC 4 cut(s) 16, 88, 603, 701
HpyAV CCTTC 5 cut(s) 144, 182, 216, 567, 755
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4V TGCA 3 cut(s) 284, 506, 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 219, 413
HpyF3I CTNAG 4 cut(s) 10, 270, 311, 633
Hsp92II CATG 2 cut(s) 78, 156
Kpn2I TCCGGA 1 cut(s) 242
Kzo9I GATC 2 cut(s) 6, 235
LmnI GCTCC 3 cut(s) 150, 182, 435
Lsp1109I GCAGC 2 cut(s) 161, 778
LweI GCATC 2 cut(s) 201, 425
MaeI CTAG 5 cut(s) 29, 422, 638, 656, 665
MaeIII GTNAC 2 cut(s) 156, 200
MalI GATC 2 cut(s) 8, 237
MboI GATC 2 cut(s) 6, 235
MboII GAAGA 4 cut(s) 16, 273, 517, 770
MflI RGATCY 1 cut(s) 6
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 9 cut(s) 140, 293, 361, 479, 507, 571, 624, 644, 813
MluNI TGGCCA 1 cut(s) 498
MmeI TCCRAC 3 cut(s) 192, 555, 723
MnlI CCTC 6 cut(s) 44, 62, 175, 279, 395, 642
Mox20I TGGCCA 1 cut(s) 498
MroI TCCGGA 1 cut(s) 242
MscI TGGCCA 1 cut(s) 498
MseI TTAA 2 cut(s) 360, 678
Msp20I TGGCCA 1 cut(s) 498
MspI CCGG 2 cut(s) 243, 753
Mva1269I GAATGC 1 cut(s) 336
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 2 cut(s) 219, 413
NdeII GATC 2 cut(s) 6, 235
NheI GCTAGC 1 cut(s) 637
NlaIII CATG 2 cut(s) 78, 156
NlaIV GGNNCC 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 156, 200
NspI RCATGY 1 cut(s) 78
PaeI GCATGC 1 cut(s) 78
PctI GAATGC 1 cut(s) 336
PfeI GAWTC 2 cut(s) 553, 612
PkrI GCNGC 2 cut(s) 176, 768
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 130
PsuI RGATCY 1 cut(s) 6
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
SalI GTCGAC 1 cut(s) 601
SaqAI TTAA 2 cut(s) 360, 678
SatI GCNGC 2 cut(s) 175, 767
Sau3AI GATC 2 cut(s) 6, 235
Sau96I GGNCC 1 cut(s) 130
SfaNI GCATC 2 cut(s) 201, 425
SinI GGWCC 1 cut(s) 130
SphI GCATGC 1 cut(s) 78
Sse9I AATT 9 cut(s) 140, 293, 361, 479, 507, 571, 624, 644, 813
SsiI CCGC 1 cut(s) 180
SspMI CTAG 5 cut(s) 29, 422, 638, 656, 665
TaaI ACNGT 1 cut(s) 592
TaqI TCGA 4 cut(s) 347, 456, 602, 610
TasI AATT 9 cut(s) 140, 293, 361, 479, 507, 571, 624, 644, 813
TfiI GAWTC 2 cut(s) 553, 612
Tru1I TTAA 2 cut(s) 360, 678
Tru9I TTAA 2 cut(s) 360, 678
TscAI CASTG 1 cut(s) 520
TseFI GTSAC 2 cut(s) 156, 200
TseI GCWGC 2 cut(s) 174, 766
Tsp45I GTSAC 2 cut(s) 156, 200
TspDTI ATGAA 5 cut(s) 17, 141, 604, 663, 770
TspGWI ACGGA 2 cut(s) 330, 762
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 130
XapI RAATTY 1 cut(s) 624
XbaI TCTAGA 1 cut(s) 664
XceI RCATGY 1 cut(s) 78
XmiI GTMKAC 2 cut(s) 87, 602
XspI CTAG 5 cut(s) 29, 422, 638, 656, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.