pycom15g22020

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016729
Physical Location & Seq
Reverse (-)
16067413 .. 16068906
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g22020.1

Sequence Viewer

Length: 1317 bp
ATGAAGATCTCAGTTCACAACAAAACCCTAGTCCTCTTTCTCCCACAACTCTTCTCACTCCTTGTCTCCAAAGCATGCCATTCAGTAGATAAACAAGCACTTCTCCATTTCAAGCACAACATCATTTCGGACCCTTCTAAACTGCTCCATTCATGGTCACTATCCTCCGACTGCTGCTCCGCTTGGGAAGGCGTCGCGTGTAACCCTTCTGGCAGGGGTCCAATACCACCAAAATTTGGGGAGCTATCTCACCACACTCATCTCTTCCTTGATTCAAACAAGCTCTTTGGCTCGATACCAACAACATTTCGCTACCTTTTTAAATTGGAGAAGCTCTATATTGGTAACAATTACATATCTGGTGTTGTCCCTTCATCTGTTTTTGGATCTTTGAGCTCACTTTTGGAATTAGGCCTATCGGGAAATAGGTTATCGGGGCCAATTCCAACTTCAATTGGGAAGTTGGTTTTGCTAACAAAGCTTGATCTACATGGAAACAATATATCCGGAAGCATTCCAGTCTCCACTGGCAAGCTTAAGAGCTTAACATATCTTGATCTATCCGGAAATCGAATAAGTGGTAGACTACCTCAGTCCATTGGTGCACTTTCGCAATTAGTCTTGCTGTATCTCAATCATAATCAGCTCAATGGAAGCATTCCTTCTTCGATTTCTGGGCTTAATTCTCCGCGGTTTTGTCAGTTATCCCAAAACAAGCTCGCAGGTGCTTTGCCAGCATCACTAGGCCAGCTCCCAAAGATAGAAAGGAACTTTGCAATTCTCACTGACATTTTCTTCTCCAACAATCATTTTATAGGCAAGATTCCTTCAAGTGTTTCCAATTTACTTAACCTACAAACACTAGATTTGTTGACAAATCGATTCATTGGTCAAATTCCACCTCAGCTAGCAAAATTACAAAGACTAGACACTCTAGACATTTCATTTAATCCTTTGGGATTGGTTAGTGTACCAAGCTTTTTTGCAAGATTGAAACTTTTTCGACTCTTGTTGGAAAAAACCGACATTGAAGGGCAGCTTCCGAGTTGGTTATCTTCATCATATGTCTCCATATTTGACTTATCAAGCAATTCCTGGACTGGAAACTTACCTCATCGGATAGGCAACATGACTAGCCTTTCAATTTTGAACTTTTCAAACAATGGTTTCCACTCATCAATCCTAGCTGAATTCAGAAATCTTTCACTCCTAATGGATCTTGATCTGCATCTTAACATGTTCACCGGCCCAATCCATGAGGACACAATGGGAGATGTCAAAAACTTACCACACTTTTTCCTTCAAGCAATAACATGA

Protein Analysis

439

Amino Acids

48.08

Weight (kDa)

9.31

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 1.1e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 86 - 144 5.6e-07 Leucine rich repeat
LRR_8 PF13855 108 - 168 2.3e-09 Leucine rich repeat
LRR_14 PF23598 151 - 290 3.9e-10 Leucine-rich repeat region
LRR_8 PF13855 158 - 216 4.5e-09 Leucine rich repeat
LRR_4 PF12799 158 - 192 1.1e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 181 - 240 3.2e-06 Leucine rich repeat
LRR_14 PF23598 278 - 415 3.7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016729)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12240
malus_domestica MD15G1249900.v1.1
prunus_persica Prupe.7G164400_v2.0.a1
pyrus_communis pycom02g10760 pycom15g22020
rosa_chinensis RchiOBHm_Chr2g0100021
rosa_laevigata RLG00000016930
rosa_roxburghii Rroxscaffold_2G00142630
rosa_rugosa Rorug02G0084700
rosa_samantha Rh2AG133800 Rh2BG137500 Rh2CG138900 Rh2DG139200
rosa_wichuraiana Rw2G010430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 713
Acc36I ACCTGC 1 cut(s) 713
AccB7I CCANNNNNTGG 1 cut(s) 236
AccI GTMKAC 1 cut(s) 583
AccII CGCG 2 cut(s) 197, 691
AccIII TCCGGA 2 cut(s) 506, 563
AciI CCGC 3 cut(s) 180, 689, 691
AclWI GGATC 2 cut(s) 394, 1224
AcsI RAATTY 3 cut(s) 233, 894, 1190
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 1 cut(s) 972
AfiI CCNNNNNNNGG 1 cut(s) 236
AflII CTTAAG 1 cut(s) 536
AflIII ACRYGT 1 cut(s) 1236
AjnI CCWGG 1 cut(s) 1094
AjuI GAANNNNNNNTTGG 2 cut(s) 433, 465
Alw21I GWGCWC 2 cut(s) 398, 607
Alw26I GTCTC 3 cut(s) 70, 526, 1072
Alw44I GTGCAC 1 cut(s) 603
AlwI GGATC 2 cut(s) 394, 1224
Aor13HI TCCGGA 2 cut(s) 506, 563
AoxI GGCC 4 cut(s) 412, 437, 745, 1246
ApaLI GTGCAC 1 cut(s) 603
ApeKI GCWGC 2 cut(s) 174, 1036
ApoI RAATTY 3 cut(s) 233, 894, 1190
ArsI GACNNNNNNTTYG 2 cut(s) 15, 47
Asp700I GAANNNNTTC 1 cut(s) 1201
AspS9I GGNCC 4 cut(s) 130, 218, 437, 1247
AsuHPI GGTGA 2 cut(s) 242, 1234
AsuNHI GCTAGC 1 cut(s) 907
AvaII GGWCC 2 cut(s) 130, 218
BaeGI GKGCMC 1 cut(s) 607
BanII GRGCYC 1 cut(s) 398
Bbv12I GWGCWC 2 cut(s) 398, 607
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 2 cut(s) 161, 1048
BciT130I CCWGG 1 cut(s) 1096
BcoDI GTCTC 3 cut(s) 70, 526, 1072
BfaI CTAG 8 cut(s) 29, 743, 863, 908, 926, 935, 1134, 1184
BfrI CTTAAG 1 cut(s) 536
BfuAI ACCTGC 1 cut(s) 713
BglII AGATCT 1 cut(s) 6
BisI GCNGC 2 cut(s) 175, 1037
BlsI GCNGC 2 cut(s) 176, 1038
Bme1390I CCNGG 1 cut(s) 1096
Bme18I GGWCC 2 cut(s) 130, 218
BmgT120I GGNCC 4 cut(s) 130, 218, 437, 1247
BmiI GGNNCC 3 cut(s) 132, 219, 438
BmrFI CCNGG 1 cut(s) 1096
BmsI GCATC 2 cut(s) 746, 1237
BmtI GCTAGC 1 cut(s) 911
Bpu10I CCTNAGC 1 cut(s) 903
Bsa29I ATCGAT 1 cut(s) 880
BsaBI GATNNNNATC 2 cut(s) 1221, 1227
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 689
BsaWI WCCGGW 2 cut(s) 506, 563
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse118I RCCGGY 1 cut(s) 1244
Bse1I ACTGG 3 cut(s) 518, 532, 1105
Bse8I GATNNNNATC 2 cut(s) 1221, 1227
BseAI TCCGGA 2 cut(s) 506, 563
BseBI CCWGG 1 cut(s) 1096
BseCI ATCGAT 1 cut(s) 880
BseDI CCNNGG 1 cut(s) 689
BseJI GATNNNNATC 2 cut(s) 1221, 1227
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMII CTCAG 3 cut(s) 24, 605, 917
BseNI ACTGG 3 cut(s) 518, 532, 1105
BseSI GKGCMC 1 cut(s) 607
BseXI GCAGC 2 cut(s) 161, 1048
Bsh1236I CGCG 2 cut(s) 197, 691
BshFI GGCC 4 cut(s) 414, 439, 747, 1248
BshVI ATCGAT 1 cut(s) 880
BsiHKAI GWGCWC 2 cut(s) 398, 607
BsiSI CCGG 3 cut(s) 507, 564, 1245
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 3 cut(s) 70, 526, 1072
BsmFI GGGAC 1 cut(s) 353
BsmI GAATGC 2 cut(s) 513, 657
BsnI GGCC 4 cut(s) 414, 439, 747, 1248
Bsp1286I GDGCHC 2 cut(s) 398, 607
Bsp13I TCCGGA 2 cut(s) 506, 563
Bsp143I GATC 6 cut(s) 6, 386, 484, 556, 1216, 1222
BspACI CCGC 3 cut(s) 180, 689, 691
BspANI GGCC 4 cut(s) 414, 439, 747, 1248
BspCNI CTCAG 3 cut(s) 23, 604, 916
BspDI ATCGAT 1 cut(s) 880
BspEI TCCGGA 2 cut(s) 506, 563
BspFNI CGCG 2 cut(s) 197, 691
BspLI GGNNCC 3 cut(s) 132, 219, 438
BspMI ACCTGC 1 cut(s) 713
BspOI GCTAGC 1 cut(s) 911
BspPI GGATC 2 cut(s) 394, 1224
BspTI CTTAAG 1 cut(s) 536
BsrFI RCCGGY 1 cut(s) 1244
BsrI ACTGG 3 cut(s) 518, 532, 1105
BssAI RCCGGY 1 cut(s) 1244
BssECI CCNNGG 1 cut(s) 689
BssMI GATC 6 cut(s) 6, 386, 484, 556, 1216, 1222
BssNI GRCGYC 1 cut(s) 192
Bst2UI CCWGG 1 cut(s) 1096
Bst6I CTCTTC 2 cut(s) 56, 269
BstACI GRCGYC 1 cut(s) 192
BstAFI CTTAAG 1 cut(s) 536
BstC8I GCNNGC 6 cut(s) 76, 533, 720, 735, 749, 909
BstDEI CTNAG 3 cut(s) 10, 591, 903
BstDSI CCRYGG 1 cut(s) 689
BstFNI CGCG 2 cut(s) 197, 691
BstKTI GATC 6 cut(s) 9, 389, 487, 559, 1219, 1225
BstMAI GTCTC 3 cut(s) 70, 526, 1072
BstMBI GATC 6 cut(s) 6, 386, 484, 556, 1216, 1222
BstMWI GCNNNNNNNGC 2 cut(s) 478, 734
BstNI CCWGG 1 cut(s) 1096
BstNSI RCATGY 2 cut(s) 78, 1240
BstSCI CCNGG 1 cut(s) 1094
BstSLI GKGCMC 1 cut(s) 607
BstUI CGCG 2 cut(s) 197, 691
BstV1I GCAGC 2 cut(s) 161, 1048
BstX2I RGATCY 3 cut(s) 6, 386, 1216
BstYI RGATCY 3 cut(s) 6, 386, 1216
Bsu15I ATCGAT 1 cut(s) 880
BsuRI GGCC 4 cut(s) 414, 439, 747, 1248
BsuTUI ATCGAT 1 cut(s) 880
BtgI CCRYGG 1 cut(s) 689
BtsIMutI CAGTG 2 cut(s) 525, 783
BveI ACCTGC 1 cut(s) 713
Cac8I GCNNGC 6 cut(s) 76, 533, 720, 735, 749, 909
Cfr10I RCCGGY 1 cut(s) 1244
Cfr13I GGNCC 4 cut(s) 130, 218, 437, 1247
Cfr42I CCGCGG 1 cut(s) 692
ClaI ATCGAT 1 cut(s) 880
CseI GACGC 1 cut(s) 181
Csp6I GTAC 1 cut(s) 971
CviAII CATG 7 cut(s) 75, 153, 491, 1129, 1237, 1256, 1314
CviQI GTAC 1 cut(s) 971
DdeI CTNAG 3 cut(s) 10, 591, 903
DpnI GATC 6 cut(s) 8, 388, 486, 558, 1218, 1224
DpnII GATC 6 cut(s) 6, 386, 484, 556, 1216, 1222
DraI TTTAAA 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 56, 269
EarI CTCTTC 2 cut(s) 56, 269
Ecl136II GAGCTC 1 cut(s) 396
Eco147I AGGCCT 1 cut(s) 414
Eco24I GRGCYC 1 cut(s) 398
Eco47I GGWCC 2 cut(s) 130, 218
Eco53kI GAGCTC 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 396
EcoRI GAATTC 1 cut(s) 1190
EcoRII CCWGG 1 cut(s) 1094
EcoT38I GRGCYC 1 cut(s) 398
FaeI CATG 7 cut(s) 78, 156, 494, 1132, 1240, 1259, 1317
FalI AAGNNNNNCTT 4 cut(s) 646, 678, 1023, 1055
FaqI GGGAC 1 cut(s) 353
FatI CATG 7 cut(s) 74, 152, 490, 1128, 1236, 1255, 1313
FauNDI CATATG 1 cut(s) 1063
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 2 cut(s) 175, 1037
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 175, 1037
FspBI CTAG 8 cut(s) 29, 743, 863, 908, 926, 935, 1134, 1184
GluI GCNGC 2 cut(s) 175, 1037
HaeIII GGCC 4 cut(s) 414, 439, 747, 1248
HapII CCGG 3 cut(s) 507, 564, 1245
HgaI GACGC 1 cut(s) 181
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 7 cut(s) 78, 156, 494, 1132, 1240, 1259, 1317
HincII GTYRAC 1 cut(s) 873
HindII GTYRAC 1 cut(s) 873
HindIII AAGCTT 3 cut(s) 479, 533, 976
HinfI GANTC 4 cut(s) 272, 823, 882, 1005
HpaII CCGG 3 cut(s) 507, 564, 1245
HphI GGTGA 2 cut(s) 242, 1234
Hpy166II GTNNAC 6 cut(s) 16, 584, 605, 873, 971, 1242
Hpy188I TCNGA 5 cut(s) 130, 169, 1044, 1119, 1196
Hpy188III TCNNGA 6 cut(s) 420, 507, 554, 564, 935, 1220
Hpy8I GTNNAC 6 cut(s) 16, 584, 605, 873, 971, 1242
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 8 cut(s) 144, 182, 216, 381, 672, 837, 1025, 1310
HpyCH4V TGCA 4 cut(s) 605, 776, 986, 1228
HpyF10VI GCNNNNNNNGC 2 cut(s) 478, 734
HpyF3I CTNAG 3 cut(s) 10, 591, 903
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 7 cut(s) 78, 156, 494, 1132, 1240, 1259, 1317
Kpn2I TCCGGA 2 cut(s) 506, 563
KspI CCGCGG 1 cut(s) 692
Kzo9I GATC 6 cut(s) 6, 386, 484, 556, 1216, 1222
LmnI GCTCC 4 cut(s) 150, 182, 241, 756
Lsp1109I GCAGC 2 cut(s) 161, 1048
LweI GCATC 2 cut(s) 746, 1237
MaeI CTAG 8 cut(s) 29, 743, 863, 908, 926, 935, 1134, 1184
MaeIII GTNAC 3 cut(s) 156, 200, 344
MalI GATC 6 cut(s) 8, 388, 486, 558, 1218, 1224
MboI GATC 6 cut(s) 6, 386, 484, 556, 1216, 1222
MboII GAAGA 6 cut(s) 16, 43, 256, 657, 787, 1047
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 3 cut(s) 6, 386, 1216
MhlI GDGCHC 2 cut(s) 398, 607
MlyI GAGTC 1 cut(s) 999
MmeI TCCRAC 4 cut(s) 192, 470, 825, 993
MnlI CCTC 6 cut(s) 44, 175, 600, 912, 1122, 1252
MroI TCCGGA 2 cut(s) 506, 563
MroXI GAANNNNTTC 1 cut(s) 1201
MseI TTAA 7 cut(s) 321, 537, 545, 681, 849, 948, 1233
MspA1I CMGCKG 1 cut(s) 691
MspCI CTTAAG 1 cut(s) 536
MspI CCGG 3 cut(s) 507, 564, 1245
MspR9I CCNGG 1 cut(s) 1096
MunI CAATTG 1 cut(s) 453
Mva1269I GAATGC 2 cut(s) 513, 657
MvaI CCWGG 1 cut(s) 1096
MvnI CGCG 2 cut(s) 197, 691
MwoI GCNNNNNNNGC 2 cut(s) 478, 734
NdeI CATATG 1 cut(s) 1063
NdeII GATC 6 cut(s) 6, 386, 484, 556, 1216, 1222
NheI GCTAGC 1 cut(s) 907
NlaIII CATG 7 cut(s) 78, 156, 494, 1132, 1240, 1259, 1317
NlaIV GGNNCC 3 cut(s) 132, 219, 438
NmuCI GTSAC 1 cut(s) 156
NspI RCATGY 2 cut(s) 78, 1240
PaeI GCATGC 1 cut(s) 78
PaqCI CACCTGC 1 cut(s) 713
PceI AGGCCT 1 cut(s) 414
PciI ACATGT 1 cut(s) 1236
PctI GAATGC 2 cut(s) 513, 657
PdmI GAANNNNTTC 1 cut(s) 1201
PfeI GAWTC 3 cut(s) 272, 823, 882
PflMI CCANNNNNTGG 1 cut(s) 236
PfoI TCCNGGA 1 cut(s) 1094
PkrI GCNGC 2 cut(s) 176, 1038
PleI GAGTC 1 cut(s) 999
PpsI GAGTC 1 cut(s) 999
PscI ACATGT 1 cut(s) 1236
Psp124BI GAGCTC 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 1094
PspGI CCWGG 1 cut(s) 1094
PspN4I GGNNCC 3 cut(s) 132, 219, 438
PspPI GGNCC 4 cut(s) 130, 218, 437, 1247
PsuI RGATCY 3 cut(s) 6, 386, 1216
RsaI GTAC 1 cut(s) 972
RsaNI GTAC 1 cut(s) 971
SacI GAGCTC 1 cut(s) 398
SacII CCGCGG 1 cut(s) 692
SaqAI TTAA 7 cut(s) 321, 537, 545, 681, 849, 948, 1233
SatI GCNGC 2 cut(s) 175, 1037
Sau3AI GATC 6 cut(s) 6, 386, 484, 556, 1216, 1222
Sau96I GGNCC 4 cut(s) 130, 218, 437, 1247
SchI GAGTC 1 cut(s) 999
ScrFI CCNGG 1 cut(s) 1096
SduI GDGCHC 2 cut(s) 398, 607
SfaNI GCATC 2 cut(s) 746, 1237
Sfr303I CCGCGG 1 cut(s) 692
SgrBI CCGCGG 1 cut(s) 692
SinI GGWCC 2 cut(s) 130, 218
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
SphI GCATGC 1 cut(s) 78
SseBI AGGCCT 1 cut(s) 414
SsiI CCGC 3 cut(s) 180, 689, 691
SspMI CTAG 8 cut(s) 29, 743, 863, 908, 926, 935, 1134, 1184
SstI GAGCTC 1 cut(s) 398
StuI AGGCCT 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 1094
TaqI TCGA 5 cut(s) 293, 571, 668, 880, 1003
TfiI GAWTC 3 cut(s) 272, 823, 882
Tru1I TTAA 7 cut(s) 321, 537, 545, 681, 849, 948, 1233
Tru9I TTAA 7 cut(s) 321, 537, 545, 681, 849, 948, 1233
TscAI CASTG 2 cut(s) 532, 790
TseFI GTSAC 1 cut(s) 156
TseI GCWGC 2 cut(s) 174, 1036
Tsp45I GTSAC 1 cut(s) 156
TspDTI ATGAA 6 cut(s) 17, 141, 363, 874, 933, 1047
TspRI CASTG 2 cut(s) 532, 790
Van91I CCANNNNNTGG 1 cut(s) 236
Vha464I CTTAAG 1 cut(s) 536
VneI GTGCAC 1 cut(s) 603
VpaK11BI GGWCC 2 cut(s) 130, 218
XapI RAATTY 3 cut(s) 233, 894, 1190
XbaI TCTAGA 1 cut(s) 934
XceI RCATGY 2 cut(s) 78, 1240
XmiI GTMKAC 1 cut(s) 583
XmnI GAANNNNTTC 1 cut(s) 1201
XspI CTAG 8 cut(s) 29, 743, 863, 908, 926, 935, 1134, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.