MD16G1056800.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0020373
Physical Location & Seq
Reverse (-)
4086947 .. 4088229
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1056800.v1.1.491

Sequence Viewer

Length: 324 bp
ATGTTGATGAACAAGTTTTTTAAAACCAGCAATGAGGTCGAATCAAAGAAGATCGAGAAAGGAATACGAGATAGCATATTAGAAATTGTTAAGAAAAGAGAAGAGAAGGCAACGAGTGGAGGAGAAGACGGCTTTGGGAGTGATTTTCTTGGATTACTTGTAAAGGCTCGCCATGATGCCAATGACAAGCAAGGGATTTCGGTTGACTATTTGGTTGATGAATGCAAGACATTTTATTTTGCTGGACAAGAAACCACTAATACTTTGCTGGCTTGGACTGTCTTTCTTCTGGCTTTCCATACGGATTGGCAGTTTGAACTTTGA

Protein Analysis

108

Amino Acids

12.23

Weight (kDa)

5.15

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 4 - 104 1.1e-10 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020373)

Species Orthologous Gene IDs
malus_domestica MD16G1056800.v1.1
rosa_laevigata RLG00000006430
rosa_multiflora Rmu_co8029904.1_g000001 Rmu_co8140092.1_g000001 Rmu_sc0002638.1_g000001 Rmu_sc0004339.1_g000017

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 1 cut(s) 317
AjuI GAANNNNNNNTTGG 2 cut(s) 117, 149
BbsI GAAGAC 1 cut(s) 132
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BmsI GCATC 1 cut(s) 166
BpiI GAAGAC 1 cut(s) 132
Bse3DI GCAATG 1 cut(s) 37
BseMI GCAATG 1 cut(s) 37
BseRI GAGGAG 1 cut(s) 135
BsmI GAATGC 1 cut(s) 227
Bsp143I GATC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 37
BssMI GATC 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 1 cut(s) 96
BstC8I GCNNGC 2 cut(s) 169, 270
BstKTI GATC 1 cut(s) 54
BstMBI GATC 1 cut(s) 51
BstV2I GAAGAC 1 cut(s) 132
Cac8I GCNNGC 2 cut(s) 169, 270
CviAII CATG 1 cut(s) 173
CviJI RGCY 4 cut(s) 132, 167, 272, 293
CviKI_1 RGCY 4 cut(s) 132, 167, 272, 293
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
DraI TTTAAA 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
FaeI CATG 1 cut(s) 176
FaiI YATR 3 cut(s) 77, 174, 300
FatI CATG 1 cut(s) 172
Hin1II CATG 1 cut(s) 176
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 1 cut(s) 41
Hpy166II GTNNAC 1 cut(s) 205
Hpy188III TCNNGA 1 cut(s) 55
Hpy8I GTNNAC 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4V TGCA 1 cut(s) 225
Hsp92II CATG 1 cut(s) 176
Kzo9I GATC 1 cut(s) 51
LpnPI CCDG 4 cut(s) 40, 228, 254, 275
LweI GCATC 1 cut(s) 166
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 4 cut(s) 61, 113, 137, 278
MluCI AATT 1 cut(s) 84
MnlI CCTC 2 cut(s) 28, 113
MseI TTAA 2 cut(s) 21, 90
Mva1269I GAATGC 1 cut(s) 227
NdeII GATC 1 cut(s) 51
NlaIII CATG 1 cut(s) 176
PctI GAATGC 1 cut(s) 227
PfeI GAWTC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 21, 90
Sau3AI GATC 1 cut(s) 51
SetI ASST 1 cut(s) 39
SfaNI GCATC 1 cut(s) 166
Sse9I AATT 1 cut(s) 84
TaaI ACNGT 1 cut(s) 280
TaqI TCGA 2 cut(s) 39, 54
TasI AATT 1 cut(s) 84
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 2 cut(s) 21, 90
Tru9I TTAA 2 cut(s) 21, 90
TspDTI ATGAA 2 cut(s) 23, 234
TspGWI ACGGA 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.