RLG00000006430

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0020373
Physical Location & Seq
Forward (+)
7089703 .. 7090995
1293 bp
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UTR
Exon/CDS
Intron
RLM00000006430

Sequence Viewer

Length: 564 bp
ATGAGCAAGGTTTTTAGAACCAGTGATGAGATTGAATCAGACAAGCTTGAGAAAGGAATACGGGACTCCATAATGGAGATGGTTAAGAAGAGAGAACAAAAGGCAGTGAATATGAGTGGGGACGAAGAAAGCTTGGGGAGTGATTTTCTAGGATTGCTTTTGAAGGCTCATCATGGTGCCGATAAGAAGAACCGGATTTCAATGAACGGATTAGTTGAAGAGTGCAGGACATTTTACTTTGCAGGGCAAGAGACCACTAATACTCTACTTGCCTGGACTGTTGTGCTTCTAGGACAACATCAAGATTGGCAAGAGGAAGCGAGAAAAGAAGTCCTACAATTATTTGGGAAACAAAATCCAAATCCTGATGACATTTCAAAACTCAAAACAAAGGCTAGAATGTGTGTGGGTAATAACTTTGCTGCCATTGAAGCAAAGATTGCTCTATCAATGATTCTACAACGTTACTCATTTACCCTTTCCCCAGGTTATATCCACTCGCCTTCTCAGCTTCTTGCTGTTCGTCCACAACATGGAGTTCGAGTAATTCTTCAGTCACTTTGA

Protein Analysis

188

Amino Acids

21.11

Weight (kDa)

8.51

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 3 - 129 1.7e-15 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020373)

Species Orthologous Gene IDs
malus_domestica MD16G1056800.v1.1
rosa_laevigata RLG00000006430
rosa_multiflora Rmu_co8029904.1_g000001 Rmu_co8140092.1_g000001 Rmu_sc0002638.1_g000001 Rmu_sc0004339.1_g000017

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AccB7I CCANNNNNTGG 1 cut(s) 533
AclI AACGTT 1 cut(s) 463
AcuI CTGAAG 1 cut(s) 536
AfiI CCNNNNNNNGG 1 cut(s) 533
AgsI TTSAA 6 cut(s) 35, 163, 201, 218, 378, 431
AjnI CCWGG 2 cut(s) 272, 484
AluBI AGCT 3 cut(s) 46, 132, 511
AluI AGCT 3 cut(s) 46, 132, 511
Alw26I GTCTC 1 cut(s) 245
ApeKI GCWGC 1 cut(s) 422
BanI GGYRCC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 409
BccI CCATC 1 cut(s) 73
BciT130I CCWGG 2 cut(s) 274, 486
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 3 cut(s) 149, 290, 396
BisI GCNGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 424
Bme1390I CCNGG 2 cut(s) 274, 486
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 2 cut(s) 274, 486
BpuEI CTTGAG 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 484
BsaWI WCCGGW 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 533
Bse1I ACTGG 1 cut(s) 21
BseBI CCWGG 2 cut(s) 274, 486
BseDI CCNNGG 1 cut(s) 484
BseLI CCNNNNNNNGG 1 cut(s) 533
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 1 cut(s) 21
BseXI GCAGC 1 cut(s) 409
BsgI GTGCAG 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 176
BsiSI CCGG 1 cut(s) 193
BslFI GGGAC 2 cut(s) 77, 134
BslI CCNNNNNNNGG 1 cut(s) 533
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 2 cut(s) 77, 134
Bso31I GGTCTC 1 cut(s) 245
BspCNI CTCAG 1 cut(s) 520
BspLI GGNNCC 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 245
BsrI ACTGG 1 cut(s) 21
BssECI CCNNGG 1 cut(s) 484
Bst2UI CCWGG 2 cut(s) 274, 486
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 2 cut(s) 83, 213
BstAPI GCANNNNNTGC 1 cut(s) 440
BstDEI CTNAG 1 cut(s) 507
BstMAI GTCTC 1 cut(s) 245
BstMWI GCNNNNNNNGC 3 cut(s) 431, 440, 508
BstNI CCWGG 2 cut(s) 274, 486
BstSCI CCNGG 2 cut(s) 272, 484
BstV1I GCAGC 1 cut(s) 409
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 28, 111
CviAII CATG 2 cut(s) 173, 533
CviJI RGCY 5 cut(s) 46, 132, 167, 395, 511
CviKI_1 RGCY 5 cut(s) 46, 132, 167, 395, 511
DdeI CTNAG 1 cut(s) 507
Eam1104I CTCTTC 2 cut(s) 83, 213
EarI CTCTTC 2 cut(s) 83, 213
Eco31I GGTCTC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 272, 484
FaeI CATG 2 cut(s) 176, 536
FaiI YATR 5 cut(s) 71, 113, 174, 492, 534
FaqI GGGAC 2 cut(s) 77, 134
FatI CATG 2 cut(s) 172, 532
Fnu4HI GCNGC 1 cut(s) 423
Fsp4HI GCNGC 1 cut(s) 423
FspBI CTAG 3 cut(s) 149, 290, 396
GluI GCNGC 1 cut(s) 423
HapII CCGG 1 cut(s) 193
Hin1II CATG 2 cut(s) 176, 536
HindIII AAGCTT 2 cut(s) 44, 130
HinfI GANTC 3 cut(s) 35, 65, 454
HpaII CCGG 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 527
Hpy188I TCNGA 1 cut(s) 40
Hpy188III TCNNGA 2 cut(s) 302, 365
Hpy8I GTNNAC 1 cut(s) 527
HpyAV CCTTC 2 cut(s) 157, 513
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 463
HpyCH4V TGCA 2 cut(s) 225, 242
HpyF10VI GCNNNNNNNGC 3 cut(s) 431, 440, 508
HpyF3I CTNAG 1 cut(s) 507
HpySE526I ACGT 1 cut(s) 463
Hsp92II CATG 2 cut(s) 176, 536
LpnPI CCDG 9 cut(s) 34, 206, 211, 228, 259, 286, 378, 471, 498
Lsp1109I GCAGC 1 cut(s) 409
MaeI CTAG 3 cut(s) 149, 290, 396
MaeII ACGT 1 cut(s) 463
MaeIII GTNAC 2 cut(s) 464, 555
MboII GAAGA 5 cut(s) 100, 137, 199, 230, 542
MluCI AATT 2 cut(s) 338, 546
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 1 cut(s) 307
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 1 cut(s) 174
MspI CCGG 1 cut(s) 193
MspR9I CCNGG 2 cut(s) 274, 486
MvaI CCWGG 2 cut(s) 274, 486
MwoI GCNNNNNNNGC 3 cut(s) 431, 440, 508
NlaIII CATG 2 cut(s) 176, 536
NlaIV GGNNCC 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 555
PfeI GAWTC 2 cut(s) 35, 454
PflMI CCANNNNNTGG 1 cut(s) 533
PkrI GCNGC 1 cut(s) 424
PleI GAGTC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 59
Psp1406I AACGTT 1 cut(s) 463
Psp6I CCWGG 2 cut(s) 272, 484
PspGI CCWGG 2 cut(s) 272, 484
PspN4I GGNNCC 1 cut(s) 178
RseI CAYNNNNRTG 1 cut(s) 174
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 1 cut(s) 423
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 274, 486
SetI ASST 6 cut(s) 12, 48, 134, 466, 490, 513
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 2 cut(s) 338, 546
SspMI CTAG 3 cut(s) 149, 290, 396
StyD4I CCNGG 2 cut(s) 272, 484
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 466
TaqI TCGA 1 cut(s) 541
TasI AATT 2 cut(s) 338, 546
TfiI GAWTC 2 cut(s) 35, 454
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 2 cut(s) 28, 111
TseFI GTSAC 1 cut(s) 555
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 1 cut(s) 555
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 2 cut(s) 28, 111
Van91I CCANNNNNTGG 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XspI CTAG 3 cut(s) 149, 290, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.