MD16G1075700.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0018720
Physical Location & Seq
Forward (+)
5303318 .. 5304271
954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1075700.v1.1.491

Sequence Viewer

Length: 291 bp
ATGTCGAGCAGAAGATCCTCTCGGTCGAGGCAGTCGTCGAGTAGGAACAACAACAGTATCAGTGATGACCAGATCACTGATCTCGTATCCAAGTTACAGCAGCTTCTTCCTGAGATTCGCCCTAGGCGTTCCAACAAGGCGTCGGCGTCGAAGGTTTTGCAAGAGACTTGCAATTATATTAGAAACTTACACAGAGAGGTGGATGACCTAAGTGAGCGCTTATCAGAGCTTTTGGCCACGACGGACATGGATAACGACCAAGCAGCCATTATTAGGAGTTTACTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

97

Amino Acids

10.98

Weight (kDa)

9.09

Isoelectric Point (pI)

90.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_ILI PF23174 21 - 95 1.5e-37 ILI bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018720)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31600
malus_domestica MD13G1074300.v1.1 MD16G1075700.v1.1
prunus_persica Prupe.1G271700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440111
rosa_samantha Rh4AG375200 Rh4BG387500 Rh4CG402000
rosa_wichuraiana Rw4G032100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 9
AcoI YGGCCR 1 cut(s) 234
AcyI GRCGYC 2 cut(s) 140, 146
AfeI AGCGCT 1 cut(s) 218
AfiI CCNNNNNNNGG 1 cut(s) 273
AluBI AGCT 2 cut(s) 103, 229
AluI AGCT 2 cut(s) 103, 229
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 1 cut(s) 9
Aor51HI AGCGCT 1 cut(s) 218
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 2 cut(s) 100, 263
AspA2I CCTAGG 1 cut(s) 122
AspLEI GCGC 1 cut(s) 219
AvrII CCTAGG 1 cut(s) 122
BaeI ACNNNNGTAYC 2 cut(s) 40, 73
BalI TGGCCA 1 cut(s) 236
BbvI GCAGC 2 cut(s) 112, 275
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BciVI GTATCC 1 cut(s) 97
BcoDI GTCTC 1 cut(s) 158
BfaI CTAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 220
BfuI GTATCC 1 cut(s) 97
BisI GCNGC 2 cut(s) 101, 264
BlnI CCTAGG 1 cut(s) 122
BlsI GCNGC 2 cut(s) 102, 265
BsaHI GRCGYC 2 cut(s) 140, 146
BsaJI CCNNGG 1 cut(s) 122
Bsc4I CCNNNNNNNGG 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 122
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMII CTCAG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 112, 275
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 1 cut(s) 236
BsiEI CGRYCG 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 273
BsmAI GTCTC 1 cut(s) 158
BsnI GGCC 1 cut(s) 236
Bsp143I GATC 3 cut(s) 14, 72, 79
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 1 cut(s) 103
BspPI GGATC 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 3 cut(s) 14, 72, 79
BssNI GRCGYC 2 cut(s) 140, 146
BssT1I CCWWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 56
BstACI GRCGYC 2 cut(s) 140, 146
BstDEI CTNAG 2 cut(s) 111, 209
BstF5I GGATG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 220
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 3 cut(s) 17, 75, 82
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 3 cut(s) 14, 72, 79
BstMCI CGRYCG 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 112, 275
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
BsuI GTATCC 1 cut(s) 97
BsuRI GGCC 1 cut(s) 236
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 67, 75
CfoI GCGC 1 cut(s) 219
CseI GACGC 2 cut(s) 129, 135
CviAII CATG 1 cut(s) 247
CviJI RGCY 4 cut(s) 103, 229, 236, 266
CviKI_1 RGCY 4 cut(s) 103, 229, 236, 266
DdeI CTNAG 2 cut(s) 111, 209
DpnI GATC 3 cut(s) 16, 74, 81
DpnII GATC 3 cut(s) 14, 72, 79
EaeI YGGCCR 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 122
Eco47III AGCGCT 1 cut(s) 218
EcoT14I CCWWGG 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 1 cut(s) 250
FaiI YATR 3 cut(s) 177, 248, 287
FatI CATG 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 101, 264
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 2 cut(s) 101, 264
FspBI CTAG 1 cut(s) 123
GlaI GCGC 1 cut(s) 218
GluI GCNGC 2 cut(s) 101, 264
HaeII RGCGCY 1 cut(s) 220
HaeIII GGCC 1 cut(s) 236
HgaI GACGC 2 cut(s) 129, 135
HhaI GCGC 1 cut(s) 219
Hin1I GRCGYC 2 cut(s) 140, 146
Hin1II CATG 1 cut(s) 250
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HinfI GANTC 1 cut(s) 115
Hpy166II GTNNAC 1 cut(s) 281
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 1 cut(s) 110
Hpy8I GTNNAC 1 cut(s) 281
Hpy99I CGWCG 4 cut(s) 40, 145, 151, 244
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4V TGCA 2 cut(s) 160, 171
HpyF3I CTNAG 2 cut(s) 111, 209
Hsp92I GRCGYC 2 cut(s) 140, 146
Hsp92II CATG 1 cut(s) 250
HspAI GCGC 1 cut(s) 217
Kzo9I GATC 3 cut(s) 14, 72, 79
LpnPI CCDG 2 cut(s) 83, 123
Lsp1109I GCAGC 2 cut(s) 112, 275
MaeI CTAG 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 93
MalI GATC 3 cut(s) 16, 74, 81
MboI GATC 3 cut(s) 14, 72, 79
MboII GAAGA 2 cut(s) 24, 98
MflI RGATCY 1 cut(s) 14
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 1 cut(s) 172
MluNI TGGCCA 1 cut(s) 236
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 3 cut(s) 21, 28, 190
Mox20I TGGCCA 1 cut(s) 236
MscI TGGCCA 1 cut(s) 236
Msp20I TGGCCA 1 cut(s) 236
NdeII GATC 3 cut(s) 14, 72, 79
NlaIII CATG 1 cut(s) 250
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 124
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 2 cut(s) 102, 265
PsuI RGATCY 1 cut(s) 14
SatI GCNGC 2 cut(s) 101, 264
Sau3AI GATC 3 cut(s) 14, 72, 79
SetI ASST 5 cut(s) 105, 156, 201, 210, 231
Sse9I AATT 1 cut(s) 172
SspMI CTAG 1 cut(s) 123
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 1 cut(s) 56
TaqI TCGA 4 cut(s) 5, 26, 38, 149
TaqII GACCGA 1 cut(s) 12
TasI AATT 1 cut(s) 172
TfiI GAWTC 1 cut(s) 115
TscAI CASTG 2 cut(s) 67, 82
TseI GCWGC 2 cut(s) 100, 263
TspGWI ACGGA 1 cut(s) 257
TspRI CASTG 2 cut(s) 67, 82
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmaJI CCTAGG 1 cut(s) 122
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.