MD16G1080000.v1.1

protein NRT1 PTR FAMILY 2.13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015608
Physical Location & Seq
Forward (+)
5608051 .. 5610469
2419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1080000.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGAGCATTGTTATTTACAGAGAAATCAAAAGCTACAATTGGGAGGTTTTTTATTTTTTATTTTTTGCAGGAAATGAATCAATTGAGAAGATGGCATCATATGGGCTGACAATAAACTTCATGGTGTATTTGGTGAGGGAGTATCACATGGAGCAGGTTCCTGCTGCCAACATGATTAACAATTGGGCATCTTCCTGCTTTTTATTGACAGTCATTGGTGGCTCCATCGCTGATATCTACCTTGGCAAATTTCTCACCATTGCTTTGGCTTCCTTTGCAACACTTCTGGGGATGCTAACAGTAACCCTGACAGCCTTTGTGCCACAACTGAGGCCTCCACCTTGCACTCTTAATGGACAACAACAGCAATGTGTCTCCAGCACAAAAACCCAGCTGGGTCTTTTGATTGTTGGGTTATGCGGGCTGGGTTTAGGAACTGGAGGAATTAGGCCCTGCAGCATTCCCTTTGGGGTTGATCAATTTGATTCCGCCACCGTCGAAGGAAGAAAATCAGTCACTAGATTTTTGAATTTGTATTACGCTATATCTTGTATGGTAATTTTGATCAACCAATCCCTAGTGATGTACATTCAAGACTCTGTGAGTTGGGCATTAGGATTTGCAATTCCTACCCTGCTCATGTCATGTGCAATTCTCCTCTTTTTTGGGGGGTCCAAAATTTACTTCTATGTGAAGCCGGAAGGAAGCACATTTTCCAGTTTTGCACAAGTTCTTGTAGCGGCATACAAGAAGCGTCATCTTAAGCTTCACAATGATGATAGGGTTCATGGTGTTTTCTCCGATGATGATTCATCAAATGGAAATGGACGTAGAGTAATCAACAAGCTTCATCTCTCAACAGAATTTAGCTTCTTGAAGAAAGCTGCTTTAGTAGTGGACAATGAACTAAAAGATGATGGGTCTTGTGCAACTCCATCGAGGCTATGTAGCATTCAACAAGTTGAAGAGGTAATACGTGTTATGAAAATCCTCCCAGTTTCTGTTTCTGGTGTCATCAGCTTGCTGGCCCTTGCCCAAGAACCAACATTTGTAGTATCACAAGCCATGAAAATGGATCGTCACATTGGACCGAATTTCGCAATCCCTGCTGGATCGGTCAAAATGATGTCAATTATGACGCTCTTTATTTCCCTCACGTTGTATGACCGTGTCTTACAACGAGCCCTAACAAAGTTCACTAAATGTGAAGATGGGATCCCACCTCTTCGAAGAATAGGAATCGGGTATTTCTTCTCTGCTATGTGCATGGTGGTGGCGGGACTAGTTGAGCAACAAAGGAGAGCTTTGGCCACATCACAAGCTTCATCGAACGGGGTTGTAGCCATGTCAGTTTTTTGGCTATTTCCACAGGAAATGTTGCTTGGACTTTGTGAGATGTTTGGCGTTGTTGGACTCCTTGAGTTTTACAACAAAGAATTTCCTGAGACCATGAAAAGTATTGGGAATTCTCTGCTCTATCTTTGCTTGGCCGGGGCTAGTTGTTTGAGTAGTGTGACGGTGAGCATTGTGCATGGTGTTACAGGAAAGCATGGCCGGCCCAACTGGTTGGACAATGATATCAATGCCGGCAGATTGGACTATTTTTACTTCCTGATTGCTGGTCTGGGAGTGGTGAATTTCTTCTATTTTCTGTTTTGTGCTCGGGGATATGCATACAAGGCTAGTGCAGAAACAGTAGAGAGTTTAGATGAGAGCTTGGGGCATCCTTTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04974 map04974
Pfam Domains
Protein Families
PTR2

Protein Analysis

579

Amino Acids

63.71

Weight (kDa)

8.08

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 84 - 519 2.3e-63 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015608)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32110 FvH4_4g32110 FvH4_4g32110
malus_domestica MD16G1079900.v1.1 MD16G1080000.v1.1
prunus_persica Prupe.1G266500_v2.0.a1 Prupe.1G266600_v2.0.a1 Prupe.1G266800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440801
rosa_laevigata RLG00000006152
rosa_multiflora Rmu_sc0000424.1_g000008
rosa_roxburghii Rroxscaffold_5G00381560
rosa_rugosa Rorug04G0328200
rosa_samantha Rh4AG380000 Rh4DG386600
rosa_wichuraiana Rw4G032710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 145
AciI CCGC 4 cut(s) 420, 489, 740, 1277
AclWI GGATC 4 cut(s) 1083, 1120, 1210, 1223
AcoI YGGCCR 3 cut(s) 1308, 1488, 1552
AcsI RAATTY 8 cut(s) 248, 529, 678, 863, 1093, 1436, 1465, 1636
AfaI GTAC 1 cut(s) 587
AflII CTTAAG 1 cut(s) 761
AflIII ACRYGT 1 cut(s) 976
AgsI TTSAA 5 cut(s) 529, 593, 877, 956, 965
AhlI ACTAGT 1 cut(s) 1282
AjuI GAANNNNNNNTTGG 4 cut(s) 668, 700, 1365, 1397
Alw21I GWGCWC 1 cut(s) 1663
Alw26I GTCTC 2 cut(s) 379, 1439
AlwI GGATC 4 cut(s) 1083, 1120, 1210, 1223
AlwNI CAGNNNCTG 1 cut(s) 1001
Ama87I CYCGRG 1 cut(s) 1662
AoxI GGCC 7 cut(s) 332, 449, 1026, 1308, 1488, 1552, 1556
ApeKI GCWGC 3 cut(s) 164, 456, 884
ApoI RAATTY 8 cut(s) 248, 529, 678, 863, 1093, 1436, 1465, 1636
Asp700I GAANNNNTTC 1 cut(s) 1640
AspS9I GGNCC 5 cut(s) 450, 672, 1027, 1088, 1557
AsuC2I CCSGG 1 cut(s) 1492
AsuHPI GGTGA 4 cut(s) 145, 247, 1531, 1645
AsuII TTCGAA 1 cut(s) 1228
AvaI CYCGRG 1 cut(s) 1662
AvaII GGWCC 2 cut(s) 672, 1088
BalI TGGCCA 1 cut(s) 1310
BamHI GGATCC 1 cut(s) 1215
BanII GRGCYC 1 cut(s) 1186
BarI GAAGNNNNNNTAC 2 cut(s) 957, 989
Bbv12I GWGCWC 1 cut(s) 1663
BbvI GCAGC 3 cut(s) 151, 468, 871
BccI CCATC 5 cut(s) 85, 233, 911, 943, 1205
BcgI CGANNNNNNTGC 2 cut(s) 918, 952
BclI TGATCA 2 cut(s) 475, 564
BcnI CCSGG 1 cut(s) 1492
BcoDI GTCTC 2 cut(s) 379, 1439
BcuI ACTAGT 1 cut(s) 1282
BfaI CTAG 5 cut(s) 519, 578, 1283, 1497, 1683
BfmI CTRYAG 1 cut(s) 454
BfrI CTTAAG 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 145
BisI GCNGC 4 cut(s) 165, 457, 741, 885
BlsI GCNGC 4 cut(s) 166, 458, 742, 886
Bme1390I CCNGG 1 cut(s) 1492
Bme18I GGWCC 2 cut(s) 672, 1088
BmeT110I CYCGRG 1 cut(s) 1662
BmgT120I GGNCC 5 cut(s) 450, 672, 1027, 1088, 1557
BmiI GGNNCC 4 cut(s) 159, 223, 673, 1217
BmrFI CCNGG 1 cut(s) 1492
BmrI ACTGGG 1 cut(s) 989
BmsI GCATC 4 cut(s) 104, 197, 282, 1732
BmuI ACTGGG 1 cut(s) 989
BpmI CTGGAG 2 cut(s) 361, 459
Bpu14I TTCGAA 1 cut(s) 1228
BpuEI CTTGAG 1 cut(s) 1439
BpuMI CCSGG 1 cut(s) 1492
BsaAI YACGTR 1 cut(s) 977
BsaI GGTCTC 1 cut(s) 1439
BsaJI CCNNGG 2 cut(s) 241, 1491
Bse118I RCCGGY 2 cut(s) 1554, 1586
Bse1I ACTGG 4 cut(s) 442, 717, 995, 1568
Bse3DI GCAATG 2 cut(s) 258, 374
BseDI CCNNGG 2 cut(s) 241, 1491
BseGI GGATG 2 cut(s) 297, 1723
BseMI GCAATG 2 cut(s) 258, 374
BseMII CTCAG 2 cut(s) 320, 1434
BseNI ACTGG 4 cut(s) 442, 717, 995, 1568
BseRI GAGGAG 1 cut(s) 647
BseXI GCAGC 3 cut(s) 151, 468, 871
BseYI CCCAGC 3 cut(s) 390, 394, 424
BsgI GTGCAG 1 cut(s) 1707
BshFI GGCC 7 cut(s) 334, 451, 1028, 1310, 1490, 1554, 1558
BsiHKAI GWGCWC 1 cut(s) 1663
BsiHKCI CYCGRG 1 cut(s) 1662
BsiSI CCGG 4 cut(s) 698, 1491, 1555, 1587
BslFI GGGAC 1 cut(s) 1293
BsmAI GTCTC 2 cut(s) 379, 1439
BsmFI GGGAC 1 cut(s) 1293
BsmI GAATGC 2 cut(s) 459, 951
BsnI GGCC 7 cut(s) 334, 451, 1028, 1310, 1490, 1554, 1558
Bso31I GGTCTC 1 cut(s) 1439
BsoBI CYCGRG 1 cut(s) 1662
Bsp119I TTCGAA 1 cut(s) 1228
Bsp1286I GDGCHC 2 cut(s) 1186, 1663
Bsp1407I TGTACA 1 cut(s) 585
Bsp143I GATC 5 cut(s) 475, 564, 1075, 1112, 1215
BspACI CCGC 4 cut(s) 420, 489, 740, 1277
BspANI GGCC 7 cut(s) 334, 451, 1028, 1310, 1490, 1554, 1558
BspCNI CTCAG 2 cut(s) 321, 1435
BspLI GGNNCC 4 cut(s) 159, 223, 673, 1217
BspMAI CTGCAG 1 cut(s) 458
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 4 cut(s) 1083, 1120, 1210, 1223
BspT104I TTCGAA 1 cut(s) 1228
BspTI CTTAAG 1 cut(s) 761
BspTNI GGTCTC 1 cut(s) 1439
BsrDI GCAATG 2 cut(s) 258, 374
BsrFI RCCGGY 2 cut(s) 1554, 1586
BsrGI TGTACA 1 cut(s) 585
BsrI ACTGG 4 cut(s) 442, 717, 995, 1568
BssAI RCCGGY 2 cut(s) 1554, 1586
BssECI CCNNGG 2 cut(s) 241, 1491
BssMI GATC 5 cut(s) 475, 564, 1075, 1112, 1215
BssT1I CCWWGG 1 cut(s) 241
Bst4CI ACNGT 6 cut(s) 211, 301, 496, 1169, 1519, 1696
Bst6I CTCTTC 2 cut(s) 960, 1230
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 1 cut(s) 1106
BstAUI TGTACA 1 cut(s) 585
BstBAI YACGTR 1 cut(s) 977
BstBI TTCGAA 1 cut(s) 1228
BstC8I GCNNGC 5 cut(s) 422, 1022, 1026, 1556, 1588
BstDEI CTNAG 2 cut(s) 329, 1443
BstF5I GGATG 2 cut(s) 297, 1723
BstKTI GATC 5 cut(s) 478, 567, 1078, 1115, 1218
BstMAI GTCTC 2 cut(s) 379, 1439
BstMBI GATC 5 cut(s) 475, 564, 1075, 1112, 1215
BstMWI GCNNNNNNNGC 4 cut(s) 275, 1106, 1555, 1679
BstSCI CCNGG 1 cut(s) 1490
BstSFI CTRYAG 1 cut(s) 454
BstV1I GCAGC 3 cut(s) 151, 468, 871
BstX2I RGATCY 1 cut(s) 1215
BstXI CCANNNNNNTGG 3 cut(s) 265, 1072, 1567
BstYI RGATCY 1 cut(s) 1215
BsuRI GGCC 7 cut(s) 334, 451, 1028, 1310, 1490, 1554, 1558
BtgZI GCGATG 1 cut(s) 211
BtsCI GGATG 2 cut(s) 297, 1723
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 5 cut(s) 422, 1022, 1026, 1556, 1588
CaiI CAGNNNCTG 1 cut(s) 1001
Cfr10I RCCGGY 2 cut(s) 1554, 1586
Cfr13I GGNCC 5 cut(s) 450, 672, 1027, 1088, 1557
CseI GACGC 2 cut(s) 743, 1147
Csp6I GTAC 1 cut(s) 586
CviQI GTAC 1 cut(s) 586
DdeI CTNAG 2 cut(s) 329, 1443
DpnI GATC 5 cut(s) 477, 566, 1077, 1114, 1217
DpnII GATC 5 cut(s) 475, 564, 1075, 1112, 1215
EaeI YGGCCR 3 cut(s) 1308, 1488, 1552
Eam1104I CTCTTC 2 cut(s) 960, 1230
EarI CTCTTC 2 cut(s) 960, 1230
EciI GGCGGA 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 241
Eco147I AGGCCT 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 1186
Eco31I GGTCTC 1 cut(s) 1439
Eco32I GATATC 2 cut(s) 235, 1579
Eco47I GGWCC 2 cut(s) 672, 1088
Eco88I CYCGRG 1 cut(s) 1662
EcoO109I RGGNCCY 1 cut(s) 450
EcoRI GAATTC 1 cut(s) 1465
EcoRV GATATC 2 cut(s) 235, 1579
EcoT14I CCWWGG 1 cut(s) 241
EcoT22I ATGCAT 1 cut(s) 1675
EcoT38I GRGCYC 1 cut(s) 1186
ErhI CCWWGG 1 cut(s) 241
FalI AAGNNNNNCTT 2 cut(s) 1288, 1320
FaqI GGGAC 1 cut(s) 1293
FauI CCCGC 2 cut(s) 413, 1270
FauNDI CATATG 1 cut(s) 100
FbaI TGATCA 2 cut(s) 475, 564
Fnu4HI GCNGC 4 cut(s) 165, 457, 741, 885
FokI GGATG 2 cut(s) 304, 1710
FriOI GRGCYC 1 cut(s) 1186
FseI GGCCGGCC 1 cut(s) 1558
Fsp4HI GCNGC 4 cut(s) 165, 457, 741, 885
FspBI CTAG 5 cut(s) 519, 578, 1283, 1497, 1683
GluI GCNGC 4 cut(s) 165, 457, 741, 885
GsaI CCCAGC 3 cut(s) 394, 398, 428
GsuI CTGGAG 2 cut(s) 361, 459
HaeIII GGCC 7 cut(s) 334, 451, 1028, 1310, 1490, 1554, 1558
HapII CCGG 4 cut(s) 698, 1491, 1555, 1587
HgaI GACGC 2 cut(s) 743, 1147
HindIII AAGCTT 3 cut(s) 764, 845, 1320
HinfI GANTC 6 cut(s) 77, 485, 596, 809, 1239, 1413
HpaII CCGG 4 cut(s) 698, 1491, 1555, 1587
HphI GGTGA 4 cut(s) 145, 247, 1531, 1645
Hpy166II GTNNAC 2 cut(s) 898, 1197
Hpy188I TCNGA 1 cut(s) 802
Hpy188III TCNNGA 4 cut(s) 593, 874, 1442, 1612
Hpy8I GTNNAC 2 cut(s) 898, 1197
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 2 cut(s) 494, 695
HpyCH4III ACNGT 6 cut(s) 211, 301, 496, 1169, 1519, 1696
HpyCH4IV ACGT 3 cut(s) 829, 976, 1157
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 1106, 1555, 1679
HpyF3I CTNAG 2 cut(s) 329, 1443
HpySE526I ACGT 3 cut(s) 829, 976, 1157
KroI GCCGGC 2 cut(s) 1554, 1586
KroNI GCCGGC 2 cut(s) 1556, 1588
Ksp22I TGATCA 2 cut(s) 475, 564
Kzo9I GATC 5 cut(s) 475, 564, 1075, 1112, 1215
LmnI GCTCC 2 cut(s) 151, 227
Lsp1109I GCAGC 3 cut(s) 151, 468, 871
LweI GCATC 4 cut(s) 104, 197, 282, 1732
MaeI CTAG 5 cut(s) 519, 578, 1283, 1497, 1683
MaeII ACGT 3 cut(s) 829, 976, 1157
MaeIII GTNAC 5 cut(s) 301, 514, 1079, 1513, 1537
MalI GATC 5 cut(s) 477, 566, 1077, 1114, 1217
MboI GATC 5 cut(s) 475, 564, 1075, 1112, 1215
MfeI CAATTG 3 cut(s) 37, 81, 181
MflI RGATCY 1 cut(s) 1215
MhlI GDGCHC 2 cut(s) 1186, 1663
MlsI TGGCCA 1 cut(s) 1310
MluNI TGGCCA 1 cut(s) 1310
MlyI GAGTC 2 cut(s) 590, 1407
MmeI TCCRAC 2 cut(s) 1390, 1548
Mox20I TGGCCA 1 cut(s) 1310
Mph1103I ATGCAT 1 cut(s) 1675
MroNI GCCGGC 2 cut(s) 1554, 1586
MroXI GAANNNNTTC 1 cut(s) 1640
MscI TGGCCA 1 cut(s) 1310
MseI TTAA 4 cut(s) 177, 351, 762, 1735
MslI CAYNNNNRTG 3 cut(s) 774, 1070, 1271
Msp20I TGGCCA 1 cut(s) 1310
MspA1I CMGCKG 1 cut(s) 394
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 4 cut(s) 698, 1491, 1555, 1587
MspR9I CCNGG 1 cut(s) 1492
MunI CAATTG 3 cut(s) 37, 81, 181
Mva1269I GAATGC 2 cut(s) 459, 951
MwoI GCNNNNNNNGC 4 cut(s) 275, 1106, 1555, 1679
NaeI GCCGGC 2 cut(s) 1556, 1588
NciI CCSGG 1 cut(s) 1492
NdeI CATATG 1 cut(s) 100
NdeII GATC 5 cut(s) 475, 564, 1075, 1112, 1215
NgoMIV GCCGGC 2 cut(s) 1554, 1586
NlaIV GGNNCC 4 cut(s) 159, 223, 673, 1217
NmuCI GTSAC 3 cut(s) 514, 1079, 1513
NsiI ATGCAT 1 cut(s) 1675
NspV TTCGAA 1 cut(s) 1228
PceI AGGCCT 1 cut(s) 334
PctI GAATGC 2 cut(s) 459, 951
PdiI GCCGGC 2 cut(s) 1556, 1588
PdmI GAANNNNTTC 1 cut(s) 1640
PfeI GAWTC 4 cut(s) 77, 485, 809, 1239
PflFI GACNNNGTC 1 cut(s) 1169
PkrI GCNGC 4 cut(s) 166, 458, 742, 886
PleI GAGTC 2 cut(s) 590, 1407
PpsI GAGTC 2 cut(s) 590, 1407
Ppu21I YACGTR 1 cut(s) 977
PspFI CCCAGC 3 cut(s) 390, 394, 424
PspN4I GGNNCC 4 cut(s) 159, 223, 673, 1217
PspPI GGNCC 5 cut(s) 450, 672, 1027, 1088, 1557
PstI CTGCAG 1 cut(s) 458
PstNI CAGNNNCTG 1 cut(s) 1001
PsuI RGATCY 1 cut(s) 1215
PsyI GACNNNGTC 1 cut(s) 1169
PvuII CAGCTG 1 cut(s) 394
RigI GGCCGGCC 1 cut(s) 1558
RsaI GTAC 1 cut(s) 587
RsaNI GTAC 1 cut(s) 586
RseI CAYNNNNRTG 3 cut(s) 774, 1070, 1271
SaqAI TTAA 4 cut(s) 177, 351, 762, 1735
SatI GCNGC 4 cut(s) 165, 457, 741, 885
Sau3AI GATC 5 cut(s) 475, 564, 1075, 1112, 1215
Sau96I GGNCC 5 cut(s) 450, 672, 1027, 1088, 1557
SchI GAGTC 2 cut(s) 590, 1407
ScrFI CCNGG 1 cut(s) 1492
SduI GDGCHC 2 cut(s) 1186, 1663
SfaNI GCATC 4 cut(s) 104, 197, 282, 1732
SfcI CTRYAG 1 cut(s) 454
SfuI TTCGAA 1 cut(s) 1228
SinI GGWCC 2 cut(s) 672, 1088
SmiMI CAYNNNNRTG 3 cut(s) 774, 1070, 1271
SmlI CTYRAG 2 cut(s) 761, 1418
SmoI CTYRAG 2 cut(s) 761, 1418
SpeI ACTAGT 1 cut(s) 1282
SseBI AGGCCT 1 cut(s) 334
SsiI CCGC 4 cut(s) 420, 489, 740, 1277
SspMI CTAG 5 cut(s) 519, 578, 1283, 1497, 1683
StuI AGGCCT 1 cut(s) 334
StyD4I CCNGG 1 cut(s) 1490
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 6 cut(s) 211, 301, 496, 1169, 1519, 1696
TaiI ACGT 3 cut(s) 832, 979, 1160
TaqI TCGA 4 cut(s) 498, 938, 1228, 1328
TaqII GACCGA 2 cut(s) 1105, 1105
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 743
TfiI GAWTC 4 cut(s) 77, 485, 809, 1239
Tru1I TTAA 4 cut(s) 177, 351, 762, 1735
Tru9I TTAA 4 cut(s) 177, 351, 762, 1735
TseFI GTSAC 3 cut(s) 514, 1079, 1513
TseI GCWGC 3 cut(s) 164, 456, 884
Tsp45I GTSAC 3 cut(s) 514, 1079, 1513
Tth111I GACNNNGTC 1 cut(s) 1169
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 2 cut(s) 672, 1088
XapI RAATTY 8 cut(s) 248, 529, 678, 863, 1093, 1436, 1465, 1636
XmnI GAANNNNTTC 1 cut(s) 1640
XspI CTAG 5 cut(s) 519, 578, 1283, 1497, 1683
Zsp2I ATGCAT 1 cut(s) 1675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.