Prupe.1G266600_v2.0.a1

protein NRT1 PTR FAMILY 2.13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0015608
Physical Location & Seq
Reverse (-)
27439942 .. 27443060
3119 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G266600.1

Sequence Viewer

Length: 1740 bp
ATGCCTTCCCATGAGTCTCCATCCTCCGAGCCATTGTTGCAACAAAAGGATGCAATCACAGTCCAAAGAAGAAGGAATGGATGGAAGGCCGTACCTTACATCTTAGGTAATGAATCAATTATAAAGATGGCAACATATGGGTTGCAAACAAATTTTATGGTGTATTTGGTGAGGGAGTATCACATGGATCAGGTTTCTGCTGCCAATATTCTCAACATTTGGACAGGCGTCTACTGTTTATTGACAGTCGTTGGTGCCTCAGTTGCTGATATCTATCTTGGCAAATTTCTCACCATTGCTCTGGCTTCCCTTGCAAGTCTTCTGGGGATGGTAACAATAACTATGTCAGCCTTTGTGCCACAATTAAGACCGCCACCTTGCATCCTTGATGGTCAAAAACATCTTGTACAATGTGTCTCCAACACAAAAACCCAACTAGGTTTCTTGCTTGCTGGGCTATCTTTGCTAGCCATAGGCACAGGTGGAATAGCGCCCTGCAGCATTCCCTTTAGCGTTGACCAATTTGACTCAACCACTGTGGAAGGAAGAAAATCAGTCCAAAGCTTTTTGAATTGGTACTATGCTTCATCCACAGTGATAATGTTGATCAACCAAACCCTAGTGGTCTACATTCAAGACTCTGTTAGCTGGGCCTTGGGTTTTGGGATCCCCACCCTCCTCATGTCATCTGTAATTCCCCTGTTTTTGGCTGGCTCCAAAATTTACCACCATGTGAAGCCAGAAGGAACAACATTTATCAGCTTTGCACAAGTTCTTGTTGCTGCCTACACGAAGCGCCATCTTAAGCTTCACAACGACGAAAGGGTATGTGGGGTTTTCTTGGATGTTTCATCGGATGGAAATGTGGTGCTCTCCAAGCACTCTCTCACTACAAAATTCAGCTCCTTGAAGAAAGCAGCTTTAGTAGTGGACGATGACCTAAAAGATGATGGGTCTTGTGCAAATCCATGGAGGCTGTGCAGCATCCAACAGGTTGAAGAAGTTATATGCTTCATGAAAATCCTCCCAATATGGGCTTCTGGTTGCATCTGCTTCATGACCTCTTCACAAGAAGGAACATTTGTAGTGTCACAAGCCCTCAAAATGGACAGGCACATTGGACCCAATTTTGAAATGCCTGCTGGGTCAATCAAAGTCATGTCATTGATCACATTATGCCTGTGCATCCCATTGTACAACCGTGTCCTTCAACCAGCCCTCAGAAAGATCACCAAGCATGAAAACGGCATCACAACTCTCCAAAGAATAGGACTTGGGTATTTGCTCTCGATTCTGTTCGCGGTGGTCGCTGGACTTGTTGAGCAACAGAGAAGAGCTTCTGCGTTGTCACAAGCTTCAGCAGATGGCGTTGCTCCCATGTCAGTGTTTTGGCTATTTCCACAGCTAATGTTGCTTGGCATGGTTGAGTTGTTTGGCTTTGTTGGGCACATTGAATTGTACAACAAGGAGTTTCCTGAGAAGATGAGGAGCATTGGGAATTCTCTGATCTATCTTTGCATTGCTGGGGCTACTTATTTGAGCACCTTGATGGTGAGCATTGTGTACAGTGTTACAGGGAAGCATGGGGAGCCCAACTGGTTGGACAATGATATCAATGCCGGCAGACTGGACTATTTCTTCATCCTTATAGCTGCGCTTGGGGTGCTGAATTTTGCGTATTTTTGGTCCTGTGCACGTGGATATACTTACACTTCCAGTGAATGCATCAAAGATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

580

Amino Acids

63.73

Weight (kDa)

8.08

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015608)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32110 FvH4_4g32110 FvH4_4g32110
malus_domestica MD16G1079900.v1.1 MD16G1080000.v1.1
prunus_persica Prupe.1G266500_v2.0.a1 Prupe.1G266600_v2.0.a1 Prupe.1G266800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440801
rosa_laevigata RLG00000006152
rosa_multiflora Rmu_sc0000424.1_g000008
rosa_roxburghii Rroxscaffold_5G00381560
rosa_rugosa Rorug04G0328200
rosa_samantha Rh4AG380000 Rh4DG386600
rosa_wichuraiana Rw4G032710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 122
AccB1I GGYRCC 1 cut(s) 254
AccI GTMKAC 2 cut(s) 231, 627
AccII CGCG 1 cut(s) 1301
AciI CCGC 2 cut(s) 371, 1301
AclWI GGATC 3 cut(s) 195, 661, 674
AcsI RAATTY 6 cut(s) 151, 284, 720, 896, 1498, 1669
AcuI CTGAAG 1 cut(s) 1341
AcvI CACGTG 1 cut(s) 1697
AcyI GRCGYC 1 cut(s) 228
AdeI CACNNNGTG 1 cut(s) 733
AfaI GTAC 6 cut(s) 93, 408, 578, 1196, 1460, 1565
AfiI CCNNNNNNNGG 3 cut(s) 706, 1033, 1105
AflII CTTAAG 1 cut(s) 803
AgsI TTSAA 7 cut(s) 571, 635, 910, 998, 1133, 1211, 1454
AjuI GAANNNNNNNTTGG 2 cut(s) 1021, 1053
Alw21I GWGCWC 3 cut(s) 873, 1544, 1696
Alw26I GTCTC 2 cut(s) 21, 421
Alw44I GTGCAC 1 cut(s) 1692
AlwI GGATC 3 cut(s) 195, 661, 674
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 2 cut(s) 87, 651
ApaLI GTGCAC 1 cut(s) 1692
ApeKI GCWGC 6 cut(s) 200, 498, 782, 917, 981, 1652
ApoI RAATTY 6 cut(s) 151, 284, 720, 896, 1498, 1669
Asp700I GAANNNNTTC 1 cut(s) 1336
AspLEI GCGC 3 cut(s) 493, 798, 1657
AspS9I GGNCC 3 cut(s) 651, 1121, 1686
AsuHPI GGTGA 4 cut(s) 181, 283, 1222, 1564
AsuNHI GCTAGC 1 cut(s) 466
AvaII GGWCC 2 cut(s) 1121, 1686
BaeGI GKGCMC 2 cut(s) 1449, 1696
BamHI GGATCC 1 cut(s) 666
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 1 cut(s) 1593
BbrPI CACGTG 1 cut(s) 1697
BbsI GAAGAC 1 cut(s) 311
Bbv12I GWGCWC 3 cut(s) 873, 1544, 1696
BbvI GCAGC 6 cut(s) 187, 510, 769, 929, 993, 1639
BceAI ACGGC 2 cut(s) 74, 1261
BclI TGATCA 2 cut(s) 606, 1167
BcoDI GTCTC 2 cut(s) 21, 421
BfaI CTAG 3 cut(s) 437, 467, 620
BfmI CTRYAG 1 cut(s) 496
BfoI RGCGCY 2 cut(s) 494, 799
BfrI CTTAAG 1 cut(s) 803
BisI GCNGC 6 cut(s) 201, 499, 783, 918, 982, 1653
BlsI GCNGC 6 cut(s) 202, 500, 784, 919, 983, 1654
Bme18I GGWCC 2 cut(s) 1121, 1686
BmgT120I GGNCC 3 cut(s) 651, 1121, 1686
BmiI GGNNCC 5 cut(s) 256, 668, 715, 1123, 1590
BmsI GCATC 7 cut(s) 40, 390, 993, 1056, 1194, 1257, 1734
BmtI GCTAGC 1 cut(s) 470
BoxI GACNNNNGTC 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 311
BsaAI YACGTR 1 cut(s) 1697
BsaBI GATNNNNATC 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 654, 968
Bsc4I CCNNNNNNNGG 3 cut(s) 706, 1033, 1105
Bse118I RCCGGY 1 cut(s) 1619
Bse1I ACTGG 3 cut(s) 1601, 1632, 1716
Bse3DI GCAATG 2 cut(s) 294, 1518
Bse8I GATNNNNATC 1 cut(s) 273
BseDI CCNNGG 2 cut(s) 654, 968
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 3 cut(s) 706, 1033, 1105
BseMI GCAATG 2 cut(s) 294, 1518
BseMII CTCAG 3 cut(s) 273, 1234, 1467
BseNI ACTGG 3 cut(s) 1601, 1632, 1716
BseRI GAGGAG 2 cut(s) 668, 1501
BseSI GKGCMC 2 cut(s) 1449, 1696
BseXI GCAGC 6 cut(s) 187, 510, 769, 929, 993, 1639
BseYI CCCAGC 4 cut(s) 452, 648, 1142, 1523
BsgI GTGCAG 1 cut(s) 1000
Bsh1236I CGCG 1 cut(s) 1301
BshFI GGCC 2 cut(s) 89, 653
BshNI GGYRCC 1 cut(s) 254
BsiHKAI GWGCWC 3 cut(s) 873, 1544, 1696
BsiSI CCGG 1 cut(s) 1620
BslI CCNNNNNNNGG 3 cut(s) 706, 1033, 1105
BsmAI GTCTC 2 cut(s) 21, 421
BsmI GAATGC 2 cut(s) 501, 1727
BsnI GGCC 2 cut(s) 89, 653
Bsp1286I GDGCHC 5 cut(s) 873, 1449, 1544, 1593, 1696
Bsp1407I TGTACA 4 cut(s) 406, 1194, 1458, 1563
Bsp143I GATC 6 cut(s) 187, 606, 666, 1167, 1227, 1506
Bsp19I CCATGG 1 cut(s) 968
BspACI CCGC 2 cut(s) 371, 1301
BspANI GGCC 2 cut(s) 89, 653
BspCNI CTCAG 3 cut(s) 272, 1233, 1468
BspFNI CGCG 1 cut(s) 1301
BspHI TCATGA 2 cut(s) 1014, 1056
BspLI GGNNCC 5 cut(s) 256, 668, 715, 1123, 1590
BspMAI CTGCAG 1 cut(s) 500
BspOI GCTAGC 1 cut(s) 470
BspPI GGATC 3 cut(s) 195, 661, 674
BspQI GCTCTTC 1 cut(s) 1327
BspT107I GGYRCC 1 cut(s) 254
BspTI CTTAAG 1 cut(s) 803
BsrDI GCAATG 2 cut(s) 294, 1518
BsrFI RCCGGY 1 cut(s) 1619
BsrGI TGTACA 4 cut(s) 406, 1194, 1458, 1563
BsrI ACTGG 3 cut(s) 1601, 1632, 1716
BssAI RCCGGY 1 cut(s) 1619
BssECI CCNNGG 2 cut(s) 654, 968
BssMI GATC 6 cut(s) 187, 606, 666, 1167, 1227, 1506
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 654, 968
Bst4CI ACNGT 7 cut(s) 61, 236, 247, 538, 595, 1202, 1568
Bst6I CTCTTC 2 cut(s) 1069, 1327
BstACI GRCGYC 1 cut(s) 228
BstAFI CTTAAG 1 cut(s) 803
BstAUI TGTACA 4 cut(s) 406, 1194, 1458, 1563
BstBAI YACGTR 1 cut(s) 1697
BstC8I GCNNGC 5 cut(s) 450, 468, 712, 1140, 1621
BstDEI CTNAG 4 cut(s) 103, 259, 1220, 1476
BstDSI CCRYGG 1 cut(s) 968
BstFNI CGCG 1 cut(s) 1301
BstH2I RGCGCY 2 cut(s) 494, 799
BstHHI GCGC 3 cut(s) 493, 798, 1657
BstKTI GATC 6 cut(s) 190, 609, 669, 1170, 1230, 1509
BstMAI GTCTC 2 cut(s) 21, 421
BstMBI GATC 6 cut(s) 187, 606, 666, 1167, 1227, 1506
BstPAI GACNNNNGTC 1 cut(s) 227
BstSFI CTRYAG 1 cut(s) 496
BstSLI GKGCMC 2 cut(s) 1449, 1696
BstUI CGCG 1 cut(s) 1301
BstV1I GCAGC 6 cut(s) 187, 510, 769, 929, 993, 1639
BstV2I GAAGAC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 666
BstXI CCANNNNNNTGG 2 cut(s) 301, 1600
BstYI RGATCY 1 cut(s) 666
BsuRI GGCC 2 cut(s) 89, 653
BtgI CCRYGG 1 cut(s) 968
BtsIMutI CAGTG 5 cut(s) 534, 600, 1389, 1573, 1723
Cac8I GCNNGC 5 cut(s) 450, 468, 712, 1140, 1621
CaiI CAGNNNCTG 1 cut(s) 266
CciI TCATGA 2 cut(s) 1014, 1056
CfoI GCGC 3 cut(s) 493, 798, 1657
Cfr10I RCCGGY 1 cut(s) 1619
Cfr13I GGNCC 3 cut(s) 651, 1121, 1686
CseI GACGC 1 cut(s) 217
Csp6I GTAC 6 cut(s) 92, 407, 577, 1195, 1459, 1564
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviQI GTAC 6 cut(s) 92, 407, 577, 1195, 1459, 1564
DdeI CTNAG 4 cut(s) 103, 259, 1220, 1476
DpnI GATC 6 cut(s) 189, 608, 668, 1169, 1229, 1508
DpnII GATC 6 cut(s) 187, 606, 666, 1167, 1227, 1506
DraIII CACNNNGTG 1 cut(s) 733
Eam1104I CTCTTC 2 cut(s) 1069, 1327
EarI CTCTTC 2 cut(s) 1069, 1327
Eco130I CCWWGG 2 cut(s) 654, 968
Eco24I GRGCYC 1 cut(s) 1593
Eco32I GATATC 2 cut(s) 271, 1612
Eco47I GGWCC 2 cut(s) 1121, 1686
Eco57I CTGAAG 1 cut(s) 1341
Eco72I CACGTG 1 cut(s) 1697
EcoRI GAATTC 1 cut(s) 1498
EcoRV GATATC 2 cut(s) 271, 1612
EcoT14I CCWWGG 2 cut(s) 654, 968
EcoT22I ATGCAT 1 cut(s) 1727
EcoT38I GRGCYC 1 cut(s) 1593
ErhI CCWWGG 2 cut(s) 654, 968
FauNDI CATATG 1 cut(s) 136
FbaI TGATCA 2 cut(s) 606, 1167
FblI GTMKAC 2 cut(s) 231, 627
Fnu4HI GCNGC 6 cut(s) 201, 499, 783, 918, 982, 1653
FriOI GRGCYC 1 cut(s) 1593
Fsp4HI GCNGC 6 cut(s) 201, 499, 783, 918, 982, 1653
FspBI CTAG 3 cut(s) 437, 467, 620
GlaI GCGC 3 cut(s) 492, 797, 1656
GluI GCNGC 6 cut(s) 201, 499, 783, 918, 982, 1653
GsaI CCCAGC 4 cut(s) 456, 652, 1146, 1527
HaeII RGCGCY 2 cut(s) 494, 799
HaeIII GGCC 2 cut(s) 89, 653
HapII CCGG 1 cut(s) 1620
HgaI GACGC 1 cut(s) 217
HhaI GCGC 3 cut(s) 493, 798, 1657
Hin1I GRCGYC 1 cut(s) 228
Hin6I GCGC 3 cut(s) 491, 796, 1655
HinP1I GCGC 3 cut(s) 491, 796, 1655
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HindIII AAGCTT 3 cut(s) 562, 806, 1353
HinfI GANTC 5 cut(s) 14, 113, 527, 638, 1291
HpaII CCGG 1 cut(s) 1620
HphI GGTGA 4 cut(s) 181, 283, 1222, 1564
Hpy166II GTNNAC 6 cut(s) 232, 517, 628, 931, 1564, 1694
Hpy188I TCNGA 4 cut(s) 28, 856, 1223, 1506
Hpy188III TCNNGA 5 cut(s) 635, 1015, 1057, 1288, 1475
Hpy8I GTNNAC 6 cut(s) 232, 517, 628, 931, 1564, 1694
Hpy99I CGWCG 1 cut(s) 821
HpyAV CCTTC 7 cut(s) 15, 66, 79, 536, 737, 1067, 1217
HpyCH4III ACNGT 7 cut(s) 61, 236, 247, 538, 595, 1202, 1568
HpyCH4IV ACGT 1 cut(s) 1696
HpyF3I CTNAG 4 cut(s) 103, 259, 1220, 1476
HpySE526I ACGT 1 cut(s) 1696
Hsp92I GRCGYC 1 cut(s) 228
HspAI GCGC 3 cut(s) 491, 796, 1655
KroI GCCGGC 1 cut(s) 1619
KroNI GCCGGC 1 cut(s) 1621
Ksp22I TGATCA 2 cut(s) 606, 1167
Kzo9I GATC 6 cut(s) 187, 606, 666, 1167, 1227, 1506
LguI GCTCTTC 1 cut(s) 1327
LmnI GCTCC 5 cut(s) 719, 908, 1378, 1488, 1588
Lsp1109I GCAGC 6 cut(s) 187, 510, 769, 929, 993, 1639
LweI GCATC 7 cut(s) 40, 390, 993, 1056, 1194, 1257, 1734
MaeI CTAG 3 cut(s) 437, 467, 620
MaeII ACGT 1 cut(s) 1696
MaeIII GTNAC 4 cut(s) 331, 1089, 1347, 1570
MalI GATC 6 cut(s) 189, 608, 668, 1169, 1229, 1508
MboI GATC 6 cut(s) 187, 606, 666, 1167, 1227, 1506
MboII GAAGA 9 cut(s) 81, 311, 558, 922, 1010, 1056, 1344, 1492, 1630
MflI RGATCY 1 cut(s) 666
MhlI GDGCHC 5 cut(s) 873, 1449, 1544, 1593, 1696
MlyI GAGTC 3 cut(s) 23, 521, 632
MmeI TCCRAC 3 cut(s) 444, 1012, 1581
Mph1103I ATGCAT 1 cut(s) 1727
MroNI GCCGGC 1 cut(s) 1619
MroXI GAANNNNTTC 1 cut(s) 1336
MseI TTAA 2 cut(s) 365, 804
MslI CAYNNNNRTG 2 cut(s) 1382, 1547
MspCI CTTAAG 1 cut(s) 803
MspI CCGG 1 cut(s) 1620
Mva1269I GAATGC 2 cut(s) 501, 1727
MvnI CGCG 1 cut(s) 1301
NaeI GCCGGC 1 cut(s) 1621
NcoI CCATGG 1 cut(s) 968
NdeI CATATG 1 cut(s) 136
NdeII GATC 6 cut(s) 187, 606, 666, 1167, 1227, 1506
NgoMIV GCCGGC 1 cut(s) 1619
NheI GCTAGC 1 cut(s) 466
NlaIV GGNNCC 5 cut(s) 256, 668, 715, 1123, 1590
NmuCI GTSAC 2 cut(s) 1089, 1347
NsiI ATGCAT 1 cut(s) 1727
PagI TCATGA 2 cut(s) 1014, 1056
PciSI GCTCTTC 1 cut(s) 1327
PctI GAATGC 2 cut(s) 501, 1727
PdiI GCCGGC 1 cut(s) 1621
PdmI GAANNNNTTC 1 cut(s) 1336
PfeI GAWTC 2 cut(s) 113, 1291
PkrI GCNGC 6 cut(s) 202, 500, 784, 919, 983, 1654
PleI GAGTC 3 cut(s) 22, 521, 632
PmaCI CACGTG 1 cut(s) 1697
PmlI CACGTG 1 cut(s) 1697
PpsI GAGTC 3 cut(s) 22, 521, 632
Ppu21I YACGTR 1 cut(s) 1697
PshAI GACNNNNGTC 1 cut(s) 227
PsiI TTATAA 1 cut(s) 122
PspCI CACGTG 1 cut(s) 1697
PspFI CCCAGC 4 cut(s) 452, 648, 1142, 1523
PspN4I GGNNCC 5 cut(s) 256, 668, 715, 1123, 1590
PspPI GGNCC 3 cut(s) 651, 1121, 1686
PstI CTGCAG 1 cut(s) 500
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 1 cut(s) 666
RsaI GTAC 6 cut(s) 93, 408, 578, 1196, 1460, 1565
RsaNI GTAC 6 cut(s) 92, 407, 577, 1195, 1459, 1564
RseI CAYNNNNRTG 2 cut(s) 1382, 1547
SapI GCTCTTC 1 cut(s) 1327
SaqAI TTAA 2 cut(s) 365, 804
SatI GCNGC 6 cut(s) 201, 499, 783, 918, 982, 1653
Sau3AI GATC 6 cut(s) 187, 606, 666, 1167, 1227, 1506
Sau96I GGNCC 3 cut(s) 651, 1121, 1686
SchI GAGTC 3 cut(s) 23, 521, 632
SduI GDGCHC 5 cut(s) 873, 1449, 1544, 1593, 1696
SfaNI GCATC 7 cut(s) 40, 390, 993, 1056, 1194, 1257, 1734
SfcI CTRYAG 1 cut(s) 496
SinI GGWCC 2 cut(s) 1121, 1686
SmiMI CAYNNNNRTG 2 cut(s) 1382, 1547
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
SsiI CCGC 2 cut(s) 371, 1301
SspI AATATT 1 cut(s) 208
SspMI CTAG 3 cut(s) 437, 467, 620
StyI CCWWGG 2 cut(s) 654, 968
TaaI ACNGT 7 cut(s) 61, 236, 247, 538, 595, 1202, 1568
TaiI ACGT 1 cut(s) 1699
TaqI TCGA 1 cut(s) 1289
TatI WGTACW 4 cut(s) 406, 1194, 1458, 1563
TfiI GAWTC 2 cut(s) 113, 1291
Tru1I TTAA 2 cut(s) 365, 804
Tru9I TTAA 2 cut(s) 365, 804
TscAI CASTG 5 cut(s) 541, 600, 1389, 1573, 1723
TseFI GTSAC 2 cut(s) 1089, 1347
TseI GCWGC 6 cut(s) 200, 498, 782, 917, 981, 1652
Tsp45I GTSAC 2 cut(s) 1089, 1347
TspDTI ATGAA 8 cut(s) 126, 576, 840, 1003, 1031, 1045, 1254, 1630
TspRI CASTG 5 cut(s) 541, 600, 1389, 1573, 1723
Vha464I CTTAAG 1 cut(s) 803
VneI GTGCAC 1 cut(s) 1692
VpaK11BI GGWCC 2 cut(s) 1121, 1686
XapI RAATTY 6 cut(s) 151, 284, 720, 896, 1498, 1669
XmiI GTMKAC 2 cut(s) 231, 627
XmnI GAANNNNTTC 1 cut(s) 1336
XspI CTAG 3 cut(s) 437, 467, 620
Zsp2I ATGCAT 1 cut(s) 1727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.