MD16G1085800.v1.1

Leucine Rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007885
Physical Location & Seq
Forward (+)
5998100 .. 6003493
5394 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1085800.v1.1.491

Sequence Viewer

Length: 1779 bp
ATGGGCTCCACTTCCGCCATCTCTCTCTGCTCTCATCCCAAGATTTTACTGCGGCCCCAAAGGTCTGGGAGTGCTTTTCTGGGCTATCGTGGGCAGGGCGTGGTTTTACCTGCTATCCCACCGCGAAGGAGGAGATGCTTGGCGGTAAAAGCCGCTTCTAGGCCATCCGGTGCTCGAAGAGTGTACAGAGAATCGCAGAGCGGCGGCTCATTGTCTGTTGCTCCTGTCAAGCAGCTTGCTTCCTTCGTTGTTCCCGCCGCCTCCTTCTTGGCCGTCACTTTTGTTTTGTGGAAATTGGTGGAGAAAATTCTTGTTCCAAAGCGTCAGAGGACTTCATCGGTAGAGAGCAATACGCCTGCACAAGGGATGAAATGGTCTTTTGCCGCCGGTACGAATCTGTTATCGGGTCTGGGGGCAAAGATTGAAAGAGAGTCAAAGCTGAAGCTCAATGACTTTGCCAAGGAACTTAGGTCATTCAGAAGTGTTGACCTGTCAGGACGTAGCTTTGGAGATGAAGGATTGTTTTTTCTTTCAGAGAGTTTGGGTTACAATCAGACTGCTGAAGAAGTAAGTTTTGCTGCAAATGGAATAACTGGAACTGGAATGAAAGCCTTTGATGGTGTTCTTCAATCTAACATCATGTTAAAAACTCTTAATCTTTCCGGAAACCCTATTGGAGATGAGGGAGCTAAGTGCCTATGTGATGTATTGGTGAATAATAATGGTATTGAAAAGCTCCAGCTAAACAGTACTGACATAGGGGATGAGGGTGCAAAGGCTATTGCTGAAATGTTGAAGAAAAATTCAAACTTACGTGTTCTCGAACTTAACAACAATATGATCGACTACTCTGGATTTACAAGTCTTGCTGGAGCTCTTGCTGAGAATAACACAATACGAAATCTACATCTGAATGGCAATTATGGTGGTGCCCTGGGGGTCAATGCTTTAGCTAAAGGACTTGAAGGGAACAAGTCTTTAAGGGAACTTCATTTGCATGGAAATTCTATTGGAGATGAAGGAGTGAATGCTTTGATCTCAGGTTTATCATTACATAAAGGTAAACTTACACTTCTGGACATTGGCAACAACTCTTTTACTGCTAGAGGTGCTTTTCATGTTGGTGGATATATCAAAAAGACCAAAAACTTGTTATGGTTGAACCTTTACATGAACGATATAGGCGACGAGGGAGCTGAAAAAATTGCAGATGCTTTGAAGCAAAACCGGACGATAACAACCATAGACATGGGGGGAAATAACATCCATGCCAAGGGAGTCACTGCAATAGCTGAAGCATTGAAAGATAATTCTGTCATTACATTCTTGGAACTCGGTTACAATCCCATGGGACCTGATGGGGTGAAGGCTTTATCAGAAGTTCTCAAATTTCATGGAAACATAGAAACCCTTAAGCTTGGCTGGTGCCAGATTGGTGCAAAGGGTGCAGAGTTCATCGCAGATATGTTAAAATATAATACCACAATACGTGTTCTGGACTTGCGGGCAAATGGACTTCGAAATGAAGGTGCATCGTGCCTGGCTCGTAGCTTAAAAGTTGTTAATGAAGCTTTAACTTCATTAGATCTAGGGTTCAATGAAATTAGGGACGACGGGGCATTTGCAATTGCTCAAGCACTCAAGGCTAATGAAGATGTGACAATCACATCTCTGAACGTAGCCAGCAACTTCCTCACCAAATTTGGACAGAGTGCTTTGACAGATGCAAGAGACCATGTATATGAGATGAGTGAAAAGGAAATCAACATTTTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

593

Amino Acids

63.57

Weight (kDa)

8.97

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_6 PF13516 212 - 234 5.7e-07 Leucine Rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 118
AccB1I GGYRCC 2 cut(s) 929, 1425
AccBSI CCGCTC 1 cut(s) 201
AccII CGCG 1 cut(s) 124
AccIII TCCGGA 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 5 cut(s) 306, 802, 1003, 1388, 1700
AcuI CTGAAG 3 cut(s) 461, 582, 1314
AfaI GTAC 3 cut(s) 185, 391, 751
AfiI CCNNNNNNNGG 3 cut(s) 159, 362, 1273
AflII CTTAAG 1 cut(s) 1412
AflIII ACRYGT 2 cut(s) 814, 1489
AjnI CCWGG 2 cut(s) 933, 1539
Alw21I GWGCWC 2 cut(s) 175, 877
Alw26I GTCTC 1 cut(s) 1725
Aor13HI TCCGGA 1 cut(s) 662
AoxI GGCC 3 cut(s) 53, 161, 270
ApeKI GCWGC 2 cut(s) 232, 578
ApoI RAATTY 5 cut(s) 306, 802, 1003, 1388, 1700
ArsI GACNNNNNNTTYG 2 cut(s) 1604, 1636
AspS9I GGNCC 2 cut(s) 54, 1352
AsuHPI GGTGA 3 cut(s) 724, 1375, 1687
AsuII TTCGAA 1 cut(s) 1519
AvaII GGWCC 1 cut(s) 1352
BaeGI GKGCMC 1 cut(s) 934
BanI GGYRCC 2 cut(s) 929, 1425
BanII GRGCYC 2 cut(s) 8, 877
Bbv12I GWGCWC 2 cut(s) 175, 877
BbvI GCAGC 2 cut(s) 244, 565
BccI CCATC 4 cut(s) 26, 172, 611, 1352
BceAI ACGGC 1 cut(s) 257
BciT130I CCWGG 2 cut(s) 935, 1541
BcoDI GTCTC 1 cut(s) 1725
BfaI CTAG 4 cut(s) 159, 1104, 1589, 1777
BfrI CTTAAG 1 cut(s) 1412
BfuAI ACCTGC 1 cut(s) 118
BglII AGATCT 1 cut(s) 1585
BisI GCNGC 8 cut(s) 53, 153, 202, 205, 233, 258, 384, 579
BlsI GCNGC 8 cut(s) 54, 154, 203, 206, 234, 259, 385, 580
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 2 cut(s) 935, 1541
Bme18I GGWCC 1 cut(s) 1352
BmgT120I GGNCC 2 cut(s) 54, 1352
BmiI GGNNCC 5 cut(s) 7, 56, 931, 1353, 1427
BmrFI CCNGG 2 cut(s) 935, 1541
BmsI GCATC 4 cut(s) 125, 1201, 1541, 1714
BpmI CTGGAG 2 cut(s) 722, 891
Bpu14I TTCGAA 1 cut(s) 1519
BpuEI CTTGAG 2 cut(s) 1617, 1625
BsaAI YACGTR 2 cut(s) 815, 1490
BsaI GGTCTC 1 cut(s) 1725
BsaJI CCNNGG 5 cut(s) 459, 933, 934, 1272, 1347
BsaWI WCCGGW 3 cut(s) 167, 662, 1227
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 362, 1273
Bse118I RCCGGY 1 cut(s) 386
Bse1I ACTGG 2 cut(s) 598, 604
BseAI TCCGGA 1 cut(s) 662
BseBI CCWGG 2 cut(s) 935, 1541
BseDI CCNNGG 5 cut(s) 459, 933, 934, 1272, 1347
BseGI GGATG 5 cut(s) 34, 164, 372, 769, 1263
BseLI CCNNNNNNNGG 3 cut(s) 159, 362, 1273
BseMII CTCAG 2 cut(s) 873, 1053
BseNI ACTGG 2 cut(s) 598, 604
BseRI GAGGAG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 934
BseXI GCAGC 2 cut(s) 244, 565
BsgI GTGCAG 2 cut(s) 342, 1467
Bsh1236I CGCG 1 cut(s) 124
BshFI GGCC 3 cut(s) 55, 163, 272
BshNI GGYRCC 2 cut(s) 929, 1425
BsiHKAI GWGCWC 2 cut(s) 175, 877
BsiSI CCGG 4 cut(s) 168, 387, 663, 1228
BslFI GGGAC 2 cut(s) 1365, 1622
BslI CCNNNNNNNGG 3 cut(s) 159, 362, 1273
BsmAI GTCTC 1 cut(s) 1725
BsmFI GGGAC 2 cut(s) 1365, 1622
BsmI GAATGC 1 cut(s) 1033
BsnI GGCC 3 cut(s) 55, 163, 272
Bso31I GGTCTC 1 cut(s) 1725
Bsp119I TTCGAA 1 cut(s) 1519
Bsp1286I GDGCHC 4 cut(s) 8, 175, 877, 934
Bsp13I TCCGGA 1 cut(s) 662
Bsp1407I TGTACA 1 cut(s) 183
Bsp143I GATC 3 cut(s) 840, 1035, 1585
Bsp19I CCATGG 1 cut(s) 1347
BspANI GGCC 3 cut(s) 55, 163, 272
BspCNI CTCAG 2 cut(s) 874, 1052
BspEI TCCGGA 1 cut(s) 662
BspFNI CGCG 1 cut(s) 124
BspLI GGNNCC 5 cut(s) 7, 56, 931, 1353, 1427
BspMI ACCTGC 1 cut(s) 118
BspT104I TTCGAA 1 cut(s) 1519
BspT107I GGYRCC 2 cut(s) 929, 1425
BspTI CTTAAG 1 cut(s) 1412
BspTNI GGTCTC 1 cut(s) 1725
BsrBI CCGCTC 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 386
BsrGI TGTACA 1 cut(s) 183
BsrI ACTGG 2 cut(s) 598, 604
BssAI RCCGGY 1 cut(s) 386
BssECI CCNNGG 5 cut(s) 459, 933, 934, 1272, 1347
BssMI GATC 3 cut(s) 840, 1035, 1585
BssT1I CCWWGG 3 cut(s) 459, 1272, 1347
Bst2UI CCWGG 2 cut(s) 935, 1541
Bst4CI ACNGT 1 cut(s) 749
Bst6I CTCTTC 1 cut(s) 172
BstAFI CTTAAG 1 cut(s) 1412
BstAPI GCANNNNNTGC 1 cut(s) 1445
BstAUI TGTACA 1 cut(s) 183
BstBAI YACGTR 2 cut(s) 815, 1490
BstBI TTCGAA 1 cut(s) 1519
BstC8I GCNNGC 4 cut(s) 237, 357, 1506, 1684
BstDEI CTNAG 4 cut(s) 467, 690, 882, 1039
BstDSI CCRYGG 1 cut(s) 1347
BstENI CCTNNNNNAGG 1 cut(s) 360
BstF5I GGATG 5 cut(s) 34, 164, 372, 769, 1263
BstFNI CGCG 1 cut(s) 124
BstKTI GATC 3 cut(s) 843, 1038, 1588
BstMAI GTCTC 1 cut(s) 1725
BstMBI GATC 3 cut(s) 840, 1035, 1585
BstMWI GCNNNNNNNGC 4 cut(s) 149, 1109, 1445, 1643
BstNI CCWGG 2 cut(s) 935, 1541
BstSCI CCNGG 2 cut(s) 933, 1539
BstSLI GKGCMC 1 cut(s) 934
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 2 cut(s) 244, 565
BstX2I RGATCY 1 cut(s) 1585
BstXI CCANNNNNNTGG 2 cut(s) 65, 1249
BstYI RGATCY 1 cut(s) 1585
BsuRI GGCC 3 cut(s) 55, 163, 272
BtgI CCRYGG 1 cut(s) 1347
BtgZI GCGATG 1 cut(s) 1441
BtsCI GGATG 5 cut(s) 34, 164, 372, 769, 1263
BtsI GCAGTG 1 cut(s) 1281
BtsIMutI CAGTG 1 cut(s) 1281
BveI ACCTGC 1 cut(s) 118
Cac8I GCNNGC 4 cut(s) 237, 357, 1506, 1684
Cfr10I RCCGGY 1 cut(s) 386
Cfr13I GGNCC 2 cut(s) 54, 1352
CseI GACGC 1 cut(s) 311
Csp6I GTAC 3 cut(s) 184, 390, 750
CspCI CAANNNNNGTGG 2 cut(s) 907, 942
CviAII CATG 9 cut(s) 640, 998, 1118, 1171, 1249, 1268, 1348, 1394, 1736
CviQI GTAC 3 cut(s) 184, 390, 750
DdeI CTNAG 4 cut(s) 467, 690, 882, 1039
DpnI GATC 3 cut(s) 842, 1037, 1587
DpnII GATC 3 cut(s) 840, 1035, 1585
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
EciI GGCGGA 1 cut(s) 4
Ecl136II GAGCTC 1 cut(s) 875
Eco130I CCWWGG 3 cut(s) 459, 1272, 1347
Eco24I GRGCYC 2 cut(s) 8, 877
Eco31I GGTCTC 1 cut(s) 1725
Eco47I GGWCC 1 cut(s) 1352
Eco53kI GAGCTC 1 cut(s) 875
Eco57I CTGAAG 3 cut(s) 461, 582, 1314
EcoICRI GAGCTC 1 cut(s) 875
EcoNI CCTNNNNNAGG 1 cut(s) 360
EcoO109I RGGNCCY 1 cut(s) 1352
EcoRII CCWGG 2 cut(s) 933, 1539
EcoT14I CCWWGG 3 cut(s) 459, 1272, 1347
EcoT38I GRGCYC 2 cut(s) 8, 877
ErhI CCWWGG 3 cut(s) 459, 1272, 1347
FaeI CATG 9 cut(s) 643, 1001, 1121, 1174, 1252, 1271, 1351, 1397, 1739
FalI AAGNNNNNCTT 2 cut(s) 1050, 1082
FaqI GGGAC 2 cut(s) 1365, 1622
FatI CATG 9 cut(s) 639, 997, 1117, 1170, 1248, 1267, 1347, 1393, 1735
FauI CCCGC 2 cut(s) 262, 1497
Fnu4HI GCNGC 8 cut(s) 53, 153, 202, 205, 233, 258, 384, 579
FokI GGATG 5 cut(s) 21, 151, 379, 776, 1250
FriOI GRGCYC 2 cut(s) 8, 877
Fsp4HI GCNGC 8 cut(s) 53, 153, 202, 205, 233, 258, 384, 579
FspBI CTAG 4 cut(s) 159, 1104, 1589, 1777
GluI GCNGC 8 cut(s) 53, 153, 202, 205, 233, 258, 384, 579
GsuI CTGGAG 2 cut(s) 722, 891
HaeIII GGCC 3 cut(s) 55, 163, 272
HapII CCGG 4 cut(s) 168, 387, 663, 1228
HgaI GACGC 1 cut(s) 311
Hin1II CATG 9 cut(s) 643, 1001, 1121, 1174, 1252, 1271, 1351, 1397, 1739
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 2 cut(s) 1415, 1569
HinfI GANTC 4 cut(s) 191, 394, 431, 1278
HpaII CCGG 4 cut(s) 168, 387, 663, 1228
HphI GGTGA 3 cut(s) 724, 1375, 1687
Hpy166II GTNNAC 3 cut(s) 184, 487, 1064
Hpy188I TCNGA 7 cut(s) 327, 479, 535, 555, 912, 1378, 1674
Hpy188III TCNNGA 6 cut(s) 495, 663, 821, 852, 1076, 1496
Hpy8I GTNNAC 3 cut(s) 184, 487, 1064
Hpy99I CGWCG 2 cut(s) 1190, 1616
HpyAV CCTTC 8 cut(s) 120, 253, 274, 509, 959, 1013, 1360, 1520
HpyCH4III ACNGT 1 cut(s) 749
HpyCH4IV ACGT 4 cut(s) 499, 814, 1489, 1677
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 1109, 1445, 1643
HpyF3I CTNAG 4 cut(s) 467, 690, 882, 1039
HpySE526I ACGT 4 cut(s) 499, 814, 1489, 1677
Hsp92II CATG 9 cut(s) 643, 1001, 1121, 1174, 1252, 1271, 1351, 1397, 1739
Kpn2I TCCGGA 1 cut(s) 662
Kzo9I GATC 3 cut(s) 840, 1035, 1585
LmnI GCTCC 6 cut(s) 11, 226, 686, 741, 872, 1193
Lsp1109I GCAGC 2 cut(s) 244, 565
LweI GCATC 4 cut(s) 125, 1201, 1541, 1714
MaeI CTAG 4 cut(s) 159, 1104, 1589, 1777
MaeII ACGT 4 cut(s) 499, 814, 1489, 1677
MaeIII GTNAC 5 cut(s) 274, 545, 1279, 1337, 1657
MalI GATC 3 cut(s) 842, 1037, 1587
MbiI CCGCTC 1 cut(s) 201
MboI GATC 3 cut(s) 840, 1035, 1585
MboII GAAGA 5 cut(s) 189, 575, 617, 808, 1664
MfeI CAATTG 1 cut(s) 1626
MflI RGATCY 1 cut(s) 1585
MhlI GDGCHC 4 cut(s) 8, 175, 877, 934
MlyI GAGTC 2 cut(s) 440, 1287
MnlI CCTC 8 cut(s) 123, 271, 321, 676, 760, 1100, 1183, 1703
MroI TCCGGA 1 cut(s) 662
MseI TTAA 9 cut(s) 644, 654, 828, 980, 1413, 1469, 1553, 1563, 1574
MslI CAYNNNNRTG 5 cut(s) 912, 996, 1122, 1247, 1740
MspCI CTTAAG 1 cut(s) 1412
MspI CCGG 4 cut(s) 168, 387, 663, 1228
MspR9I CCNGG 2 cut(s) 935, 1541
MunI CAATTG 1 cut(s) 1626
Mva1269I GAATGC 1 cut(s) 1033
MvaI CCWGG 2 cut(s) 935, 1541
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 4 cut(s) 149, 1109, 1445, 1643
NcoI CCATGG 1 cut(s) 1347
NdeII GATC 3 cut(s) 840, 1035, 1585
NlaIII CATG 9 cut(s) 643, 1001, 1121, 1174, 1252, 1271, 1351, 1397, 1739
NlaIV GGNNCC 5 cut(s) 7, 56, 931, 1353, 1427
NmuCI GTSAC 3 cut(s) 274, 1279, 1657
NspV TTCGAA 1 cut(s) 1519
PasI CCCWGGG 1 cut(s) 934
PcsI WCGNNNNNNNCGW 1 cut(s) 1182
PctI GAATGC 1 cut(s) 1033
PfeI GAWTC 2 cut(s) 191, 394
PkrI GCNGC 8 cut(s) 54, 154, 203, 206, 234, 259, 385, 580
PleI GAGTC 2 cut(s) 439, 1286
PpsI GAGTC 2 cut(s) 439, 1286
Ppu21I YACGTR 2 cut(s) 815, 1490
PpuMI RGGWCCY 1 cut(s) 1352
Psp124BI GAGCTC 1 cut(s) 877
Psp5II RGGWCCY 1 cut(s) 1352
Psp6I CCWGG 2 cut(s) 933, 1539
PspGI CCWGG 2 cut(s) 933, 1539
PspN4I GGNNCC 5 cut(s) 7, 56, 931, 1353, 1427
PspPI GGNCC 2 cut(s) 54, 1352
PspPPI RGGWCCY 1 cut(s) 1352
PsuI RGATCY 1 cut(s) 1585
RsaI GTAC 3 cut(s) 185, 391, 751
RsaNI GTAC 3 cut(s) 184, 390, 750
RseI CAYNNNNRTG 5 cut(s) 912, 996, 1122, 1247, 1740
SacI GAGCTC 1 cut(s) 877
SaqAI TTAA 9 cut(s) 644, 654, 828, 980, 1413, 1469, 1553, 1563, 1574
SatI GCNGC 8 cut(s) 53, 153, 202, 205, 233, 258, 384, 579
Sau3AI GATC 3 cut(s) 840, 1035, 1585
Sau96I GGNCC 2 cut(s) 54, 1352
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 2 cut(s) 440, 1287
ScrFI CCNGG 2 cut(s) 935, 1541
SduI GDGCHC 4 cut(s) 8, 175, 877, 934
SfaNI GCATC 4 cut(s) 125, 1201, 1541, 1714
SfuI TTCGAA 1 cut(s) 1519
SinI GGWCC 1 cut(s) 1352
SmiMI CAYNNNNRTG 5 cut(s) 912, 996, 1122, 1247, 1740
SmlI CTYRAG 3 cut(s) 1412, 1632, 1640
SmoI CTYRAG 3 cut(s) 1412, 1632, 1640
SspMI CTAG 4 cut(s) 159, 1104, 1589, 1777
SstI GAGCTC 1 cut(s) 877
StyD4I CCNGG 2 cut(s) 933, 1539
StyI CCWWGG 3 cut(s) 459, 1272, 1347
TaaI ACNGT 1 cut(s) 749
TaiI ACGT 4 cut(s) 502, 817, 1492, 1680
TaqI TCGA 4 cut(s) 175, 822, 843, 1519
TatI WGTACW 2 cut(s) 183, 749
TauI GCSGC 6 cut(s) 55, 155, 204, 207, 260, 386
TfiI GAWTC 2 cut(s) 191, 394
Tru1I TTAA 9 cut(s) 644, 654, 828, 980, 1413, 1469, 1553, 1563, 1574
Tru9I TTAA 9 cut(s) 644, 654, 828, 980, 1413, 1469, 1553, 1563, 1574
TscAI CASTG 1 cut(s) 1288
TseFI GTSAC 3 cut(s) 274, 1279, 1657
TseI GCWGC 2 cut(s) 232, 578
Tsp45I GTSAC 3 cut(s) 274, 1279, 1657
TspRI CASTG 1 cut(s) 1288
Vha464I CTTAAG 1 cut(s) 1412
VpaK11BI GGWCC 1 cut(s) 1352
XagI CCTNNNNNAGG 1 cut(s) 360
XapI RAATTY 5 cut(s) 306, 802, 1003, 1388, 1700
XspI CTAG 4 cut(s) 159, 1104, 1589, 1777
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.