Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011809

Physical Location & Seq

Genomic Coordinates Reverse (-)

6241580 .. 6245613

Gene Structure 4034 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1089900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2148 bp

ATGGATCTGGAAACTGGGGTTTATCAGAACCATATAAAGAAAGCGTCATGGAGGACAATTCTGACTCTGGCTTATCAGAGCCTCGGCGTCGTCTATGGCGATTTAAGCACTTCGCCTCTGTACGTTTACAAAAGCACATTTGCTGAAGATATCGAGCAGTCAGAGACCAACGAAGAAATCTTCGGCGTCTTGTCCTTCGTCTTCTGGACCCTCACTCTCGTCCCCCTTCTCAAATACGTGTTTATTGTGCTCAAAGCCGACGACAATGGCGAGGGAGGAACATTCGCCTTGTACTCGCTGCTGTGTCGACACGCCCGACTCAGCTCGCTGCCCAACTGCCAGTCCGCCGACGAGGAGCTCTCCGATTACAAGAAGGAACGCGCCGACACGGCGCTGCAGTCGAGCTTCAGGGCGAGGTTGAAGTCGACGTTGGAGAGGCACAGGGTGCTGCAAAGATTTTTGCTTGTTCTTGCTTTGATTGGAACTTGCATGGTGATTGGTGACGGTGTTCTCACACCAGCTCTTTCTGTATTTTCTGCGGTTTCAGGGCTGGAGTTTTCCATGTCCGAAAAGCATCATAAATTTGTAGAAGTTCCAATTGCATGCCTCATTTTGATAGGCCTGTTTGCACTTCAACATTATGGAACTCACAGGGTCGGGTTCTTGTTTGCTCCTATAGTTCTGACATGGCTTCTATGCATCGGTGCGATCGGTGTTTATAATATACTTCACTTCAATCCGAGTGTGTACAAAGCACTTTCTCCATACTATATGTACAAATTTTTAAGGAAAACTCAGAAAGGGGGCTGGATGTCCCTCGGCGGAATTTTTGTTATTGCGTTCACCTCTTTGGTTTATCCAGCTTTGGTTCTAGCATATATGGGACAGGCTGCCTATATATCTTCACACCACAATGTTGGGAAAAATTCTCAGATTGGATTTTATCCATCTGTACCAGAAAAATTGAGATGGCCCGTCCTGGTAATAGCTGTACTTGCTGCCGTAGTAGGAAGCCAGGCCATCATCACTGGAACTTTCTCAATCATCAAGCAATGTTCTGCTCTGGATTGCTTCCCAAGAGTCAAAATAGTCCATACATCATCCAAAATTCATGGTCAAATTTATATCCCGGAAATCAATTGGATCTTGATGGTGTTGTGTTTGGCTGTTACTATTGGTTTCAGAGACACCAAGCGCATGGGTAATGCTTCAGGTTTTGCAGTTATCTCGGTGATGCTGGTCACCACGTGCTTAATGTCCCTAGTTATCGTCCTATGCTGGCAGCAGAGTTTTCCTGAAGGAGCCTGGTTTCCCGTTGCTCTTGCTTTCTGTTTTTTCTTGGTCATGTATGTTTGGCATTACGGCACGATCAAAAAGTACGATTTTGAAGTCCAAAACAAGGTCTCCATTAACTGGCTGCTTAGCCTTGGTCCCAGCCTTGGCTTTGTACGTGTCCGTGGGATTGGCCTCATACACACTGATCTTGTATCTGGAATTCCGGCCATCTTCTCCCACTTTGTGTCACCAACCTTCCGGCCTTCCACCAGACCTGAGGAACGGTTTCTGGTGGGCCAAATTGGCCCTAAAGAGTACCGGCTCTACAGGTGCATTGTGAGATATGGATATCGCGACATCCACAAAGATGACATGGAATTTGAGAACGATCTTATGTGTAGCATAGCAGAGTTCATACGCTCAGGAAGCACCGAATCCAATCTTTCAATTGAAGATGCAGGGAAGGAAGATGATAAAATGACGGTAGTTGGCACACGTTCCACTCATGCAGATGGAATTCAGATGAGCGAGGACAGTCCAAACGTAGAAATGAGCCCATCAGTGCAGAGAGAAGAAAGATCGCCGCCGGTTGCCCAACGAAGGAAGAAGGTAAGATTCATTGTCCCGGAGAGTCCAAAGATCAACACGGGCGTAAGGGAGGAACTGCGGGAGCTAACGGAAGCTCGGGAAGCCGGAATAGCTTACATACTTGGAAACACATACATGAAAGCAAAGAAAGGATCCAGTTTGATGAGAAGGTTTGCGATCGACTACGGATATGAATTCTTGAGAAGGAATAGCAGGACATCCAGCTACGCGTTCAGTGTACCGCACGCGTCTACGCTGGAGGTAGGGATGATCTACCATGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

716

Amino Acids

80.33

Weight (kDa)

8.81

Isoelectric Point (pI)

50.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	23 - 279	1.3e-78	K+ potassium transporter integral membrane domain
K_trans	PF02705	279 - 430	1.5e-51	K+ potassium transporter integral membrane domain
K_trans	PF02705	431 - 474	7.4e-06	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	511 - 714	7e-13	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70300
fragaria_vesca	FvH4_4g34790 FvH4_4g34790
malus_domestica	MD13G1089700.v1.1 MD16G1089900.v1.1 MD16G1090000.v1.1
prunus_persica	Prupe.1G256100_v2.0.a1
pyrus_communis	pycom13g07820 pycom16g07700
rosa_chinensis	RchiOBHm_Chr4g0444131
rosa_laevigata	RLG00000005853
rosa_multiflora	Rmu_sc0012638.1_g000009
rosa_roxburghii	Rroxscaffold_5G00384720
rosa_rugosa	Rorug04G0350300
rosa_samantha	Rh4AG410900 Rh4BG422300 Rh4CG436700 Rh4DG417600
rosa_wichuraiana	Rw4G035300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	720
AccB7I	CCANNNNNTGG	1 cut(s)	1413
AccI	GTMKAC	3 cut(s)	307, 425, 2114
AccII	CGCG	4 cut(s)	381, 1629, 2093, 2111
AciI	CCGC	6 cut(s)	345, 539, 822, 1859, 1942, 2105
AclWI	GGATC	4 cut(s)	12, 1151, 2010, 2023
AcoI	YGGCCR	1 cut(s)	1500
AcuI	CTGAAG	4 cut(s)	165, 391, 1194, 1317
AcvI	CACGTG	1 cut(s)	1248
AcyI	GRCGYC	2 cut(s)	87, 186
AdeI	CACNNNGTG	3 cut(s)	445, 1248, 1519
AfiI	CCNNNNNNNGG	4 cut(s)	1399, 1413, 1439, 1875
AflIII	ACRYGT	5 cut(s)	237, 1450, 1769, 2091, 2109
AgsI	TTSAA	6 cut(s)	421, 635, 736, 1388, 1722, 1727
AjnI	CCWGG	3 cut(s)	978, 1014, 1304
AjuI	GAANNNNNNNTTGG	2 cut(s)	413, 445
Alw21I	GWGCWC	2 cut(s)	252, 360
Alw26I	GTCTC	3 cut(s)	158, 1179, 1408
AlwI	GGATC	4 cut(s)	12, 1151, 2010, 2023
Ama87I	CYCGRG	1 cut(s)	1959
AoxI	GGCC	8 cut(s)	619, 971, 1017, 1465, 1500, 1535, 1570, 1579
ApeKI	GCWGC	8 cut(s)	298, 328, 394, 448, 890, 998, 1282, 1417
ArsI	GACNNNNNNTTYG	2 cut(s)	1637, 1669
Asp700I	GAANNNNTTC	1 cut(s)	1560
AspLEI	GCGC	3 cut(s)	383, 394, 1197
AspS9I	GGNCC	5 cut(s)	207, 972, 1430, 1570, 1580
AsuC2I	CCSGG	2 cut(s)	1130, 1901
AsuHPI	GGTGA	6 cut(s)	505, 512, 835, 1234, 1243, 1515
AvaI	CYCGRG	1 cut(s)	1959
AvaII	GGWCC	2 cut(s)	207, 1430
AxyI	CCTNAGG	1 cut(s)	1551
BamHI	GGATCC	1 cut(s)	2015
BanII	GRGCYC	2 cut(s)	360, 1832
BbrPI	CACGTG	1 cut(s)	1248
BbsI	GAAGAC	1 cut(s)	193
Bbv12I	GWGCWC	2 cut(s)	252, 360
BbvI	GCAGC	8 cut(s)	285, 315, 381, 435, 877, 985, 1294, 1404
BccI	CCATC	7 cut(s)	955, 963, 1028, 1144, 1511, 1781, 1840
BceAI	ACGGC	3 cut(s)	405, 986, 1378
BcgI	CGANNNNNNTGC	2 cut(s)	1209, 1243
BciT130I	CCWGG	3 cut(s)	980, 1016, 1306
BcnI	CCSGG	2 cut(s)	1130, 1901
BcoDI	GTCTC	3 cut(s)	158, 1179, 1408
BfaI	CTAG	2 cut(s)	872, 1262
BfmI	CTRYAG	3 cut(s)	395, 675, 1600
BfoI	RGCGCY	1 cut(s)	395
BglI	GCCNNNNNGGC	2 cut(s)	389, 1578
BisI	GCNGC	9 cut(s)	299, 329, 395, 449, 891, 999, 1283, 1418, 1859
BlpI	GCTNAGC	1 cut(s)	1421
BlsI	GCNGC	9 cut(s)	300, 330, 396, 450, 892, 1000, 1284, 1419, 1860
Bme1390I	CCNGG	5 cut(s)	980, 1016, 1130, 1306, 1901
Bme18I	GGWCC	2 cut(s)	207, 1430
BmeT110I	CYCGRG	1 cut(s)	1959
BmgT120I	GGNCC	5 cut(s)	207, 972, 1430, 1570, 1580
BmiI	GGNNCC	4 cut(s)	209, 1303, 1432, 2017
BmrFI	CCNGG	5 cut(s)	980, 1016, 1130, 1306, 1901
BmrI	ACTGGG	1 cut(s)	24
BmsI	GCATC	4 cut(s)	583, 708, 1224, 1720
BmuI	ACTGGG	1 cut(s)	24
BpiI	GAAGAC	1 cut(s)	193
BplI	GAGNNNNNCTC	2 cut(s)	344, 376
BpmI	CTGGAG	2 cut(s)	572, 2141
Bpu10I	CCTNAGC	1 cut(s)	1696
Bpu1102I	GCTNAGC	1 cut(s)	1421
BpuEI	CTTGAG	1 cut(s)	2083
BpuMI	CCSGG	2 cut(s)	1130, 1901
BsaAI	YACGTR	3 cut(s)	238, 1248, 1451
BsaHI	GRCGYC	2 cut(s)	87, 186
BsaI	GGTCTC	2 cut(s)	158, 1408
BsaJI	CCNNGG	5 cut(s)	82, 817, 1426, 1438, 1456
BsaXI	ACNNNNNCTCC	2 cut(s)	1388, 1418
Bsc4I	CCNNNNNNNGG	4 cut(s)	1399, 1413, 1439, 1875
Bse118I	RCCGGY	2 cut(s)	1593, 1861
Bse1I	ACTGG	5 cut(s)	19, 340, 1033, 1418, 2019
Bse21I	CCTNAGG	1 cut(s)	1551
Bse3DI	GCAATG	1 cut(s)	1058
BseBI	CCWGG	3 cut(s)	980, 1016, 1306
BseDI	CCNNGG	5 cut(s)	82, 817, 1426, 1438, 1456
BseGI	GGATG	5 cut(s)	816, 1100, 1632, 2081, 2136
BseLI	CCNNNNNNNGG	4 cut(s)	1399, 1413, 1439, 1875
BseMI	GCAATG	1 cut(s)	1058
BseMII	CTCAG	5 cut(s)	334, 809, 944, 1542, 1710
BseNI	ACTGG	5 cut(s)	19, 340, 1033, 1418, 2019
BseRI	GAGGAG	1 cut(s)	368
BseXI	GCAGC	8 cut(s)	285, 315, 381, 435, 877, 985, 1294, 1404
BseYI	CCCAGC	1 cut(s)	1433
BsgI	GTGCAG	1 cut(s)	1859
Bsh1236I	CGCG	4 cut(s)	381, 1629, 2093, 2111
Bsh1285I	CGRYCG	2 cut(s)	711, 2043
BshFI	GGCC	8 cut(s)	621, 973, 1019, 1467, 1502, 1537, 1572, 1581
BsiEI	CGRYCG	2 cut(s)	711, 2043
BsiHKAI	GWGCWC	2 cut(s)	252, 360
BsiHKCI	CYCGRG	1 cut(s)	1959
BsiSI	CCGG	7 cut(s)	1130, 1499, 1534, 1594, 1862, 1901, 1968
BslFI	GGGAC	6 cut(s)	206, 799, 897, 1243, 1416, 1883
BslI	CCNNNNNNNGG	4 cut(s)	1399, 1413, 1439, 1875
BsmAI	GTCTC	3 cut(s)	158, 1179, 1408
BsmFI	GGGAC	6 cut(s)	206, 799, 897, 1243, 1416, 1883
BsnI	GGCC	8 cut(s)	621, 973, 1019, 1467, 1502, 1537, 1572, 1581
Bso31I	GGTCTC	2 cut(s)	158, 1408
BsoBI	CYCGRG	1 cut(s)	1959
Bsp1286I	GDGCHC	3 cut(s)	252, 360, 1832
Bsp1407I	TGTACA	2 cut(s)	747, 774
Bsp1720I	GCTNAGC	1 cut(s)	1421
Bsp68I	TCGCGA	1 cut(s)	1629
BspACI	CCGC	6 cut(s)	345, 539, 822, 1859, 1942, 2105
BspANI	GGCC	8 cut(s)	621, 973, 1019, 1467, 1502, 1537, 1572, 1581
BspCNI	CTCAG	5 cut(s)	333, 808, 943, 1543, 1709
BspFNI	CGCG	4 cut(s)	381, 1629, 2093, 2111
BspLI	GGNNCC	4 cut(s)	209, 1303, 1432, 2017
BspMAI	CTGCAG	1 cut(s)	399
BspPI	GGATC	4 cut(s)	12, 1151, 2010, 2023
BspTNI	GGTCTC	2 cut(s)	158, 1408
BsrDI	GCAATG	1 cut(s)	1058
BsrFI	RCCGGY	2 cut(s)	1593, 1861
BsrGI	TGTACA	2 cut(s)	747, 774
BsrI	ACTGG	5 cut(s)	19, 340, 1033, 1418, 2019
BssAI	RCCGGY	2 cut(s)	1593, 1861
BssECI	CCNNGG	5 cut(s)	82, 817, 1426, 1438, 1456
BssNI	GRCGYC	2 cut(s)	87, 186
BssT1I	CCWWGG	2 cut(s)	1426, 1438
Bst2UI	CCWGG	3 cut(s)	980, 1016, 1306
Bst4CI	ACNGT	4 cut(s)	506, 1560, 1759, 1811
BstACI	GRCGYC	2 cut(s)	87, 186
BstAPI	GCANNNNNTGC	1 cut(s)	445
BstAUI	TGTACA	2 cut(s)	747, 774
BstBAI	YACGTR	3 cut(s)	238, 1248, 1451
BstC8I	GCNNGC	4 cut(s)	326, 604, 1280, 2109
BstDEI	CTNAG	6 cut(s)	320, 795, 930, 1421, 1551, 1696
BstDSI	CCRYGG	1 cut(s)	1456
BstEII	GGTNACC	1 cut(s)	1240
BstF5I	GGATG	5 cut(s)	816, 1100, 1632, 2081, 2136
BstFNI	CGCG	4 cut(s)	381, 1629, 2093, 2111
BstH2I	RGCGCY	1 cut(s)	395
BstHHI	GCGC	3 cut(s)	383, 394, 1197
BstMAI	GTCTC	3 cut(s)	158, 1179, 1408
BstMCI	CGRYCG	2 cut(s)	711, 2043
BstMWI	GCNNNNNNNGC	8 cut(s)	105, 389, 445, 995, 1578, 1701, 1964, 1973
BstNI	CCWGG	3 cut(s)	980, 1016, 1306
BstNSI	RCATGY	1 cut(s)	606
BstPI	GGTNACC	1 cut(s)	1240
BstSCI	CCNGG	5 cut(s)	978, 1014, 1128, 1304, 1899
BstSFI	CTRYAG	3 cut(s)	395, 675, 1600
BstUI	CGCG	4 cut(s)	381, 1629, 2093, 2111
BstV1I	GCAGC	8 cut(s)	285, 315, 381, 435, 877, 985, 1294, 1404
BstV2I	GAAGAC	1 cut(s)	193
BstX2I	RGATCY	3 cut(s)	4, 1143, 2015
BstXI	CCANNNNNNTGG	2 cut(s)	917, 1198
BstYI	RGATCY	3 cut(s)	4, 1143, 2015
Bsu36I	CCTNAGG	1 cut(s)	1551
BsuRI	GGCC	8 cut(s)	621, 973, 1019, 1467, 1502, 1537, 1572, 1581
BtgI	CCRYGG	1 cut(s)	1456
BtsCI	GGATG	5 cut(s)	816, 1100, 1632, 2081, 2136
BtsIMutI	CAGTG	4 cut(s)	1026, 1476, 1842, 2104
BtuMI	TCGCGA	1 cut(s)	1629
Cac8I	GCNNGC	4 cut(s)	326, 604, 1280, 2109
CfoI	GCGC	3 cut(s)	383, 394, 1197
Cfr10I	RCCGGY	2 cut(s)	1593, 1861
Cfr13I	GGNCC	5 cut(s)	207, 972, 1430, 1570, 1580
CseI	GACGC	4 cut(s)	33, 76, 175, 2100
DdeI	CTNAG	6 cut(s)	320, 795, 930, 1421, 1551, 1696
DraIII	CACNNNGTG	3 cut(s)	445, 1248, 1519
EaeI	YGGCCR	1 cut(s)	1500
EciI	GGCGGA	2 cut(s)	334, 837
Ecl136II	GAGCTC	1 cut(s)	358
Eco130I	CCWWGG	2 cut(s)	1426, 1438
Eco147I	AGGCCT	1 cut(s)	621
Eco24I	GRGCYC	2 cut(s)	360, 1832
Eco31I	GGTCTC	2 cut(s)	158, 1408
Eco32I	GATATC	2 cut(s)	151, 1625
Eco47I	GGWCC	2 cut(s)	207, 1430
Eco53kI	GAGCTC	1 cut(s)	358
Eco57I	CTGAAG	4 cut(s)	165, 391, 1194, 1317
Eco72I	CACGTG	1 cut(s)	1248
Eco81I	CCTNAGG	1 cut(s)	1551
Eco88I	CYCGRG	1 cut(s)	1959
Eco91I	GGTNACC	1 cut(s)	1240
EcoICRI	GAGCTC	1 cut(s)	358
EcoO65I	GGTNACC	1 cut(s)	1240
EcoRI	GAATTC	3 cut(s)	1494, 1791, 2057
EcoRII	CCWGG	3 cut(s)	978, 1014, 1304
EcoRV	GATATC	2 cut(s)	151, 1625
EcoT14I	CCWWGG	2 cut(s)	1426, 1438
EcoT22I	ATGCAT	1 cut(s)	701
EcoT38I	GRGCYC	2 cut(s)	360, 1832
ErhI	CCWWGG	2 cut(s)	1426, 1438
FaqI	GGGAC	6 cut(s)	206, 799, 897, 1243, 1416, 1883
FauI	CCCGC	1 cut(s)	1935
FblI	GTMKAC	3 cut(s)	307, 425, 2114
Fnu4HI	GCNGC	9 cut(s)	299, 329, 395, 449, 891, 999, 1283, 1418, 1859
FokI	GGATG	5 cut(s)	823, 1087, 1619, 2068, 2143
FriOI	GRGCYC	2 cut(s)	360, 1832
Fsp4HI	GCNGC	9 cut(s)	299, 329, 395, 449, 891, 999, 1283, 1418, 1859
FspBI	CTAG	2 cut(s)	872, 1262
GlaI	GCGC	3 cut(s)	382, 393, 1196
GluI	GCNGC	9 cut(s)	299, 329, 395, 449, 891, 999, 1283, 1418, 1859
GsaI	CCCAGC	1 cut(s)	1437
GsuI	CTGGAG	2 cut(s)	572, 2141
HaeII	RGCGCY	1 cut(s)	395
HaeIII	GGCC	8 cut(s)	621, 973, 1019, 1467, 1502, 1537, 1572, 1581
HapII	CCGG	7 cut(s)	1130, 1499, 1534, 1594, 1862, 1901, 1968
HgaI	GACGC	4 cut(s)	33, 76, 175, 2100
HhaI	GCGC	3 cut(s)	383, 394, 1197
Hin1I	GRCGYC	2 cut(s)	87, 186
Hin6I	GCGC	3 cut(s)	381, 392, 1195
HinP1I	GCGC	3 cut(s)	381, 392, 1195
HincII	GTYRAC	2 cut(s)	308, 426
HindII	GTYRAC	2 cut(s)	308, 426
HinfI	GANTC	6 cut(s)	64, 318, 1080, 1709, 1890, 1906
HpaII	CCGG	7 cut(s)	1130, 1499, 1534, 1594, 1862, 1901, 1968
HphI	GGTGA	6 cut(s)	505, 512, 835, 1234, 1243, 1515
Hpy166II	GTNNAC	7 cut(s)	127, 308, 426, 748, 843, 2102, 2115
Hpy8I	GTNNAC	7 cut(s)	127, 308, 426, 748, 843, 2102, 2115
Hpy99I	CGWCG	4 cut(s)	92, 263, 353, 430
HpyCH4III	ACNGT	4 cut(s)	506, 1560, 1759, 1811
HpyCH4IV	ACGT	7 cut(s)	123, 237, 428, 1247, 1450, 1771, 1818
HpyF10VI	GCNNNNNNNGC	8 cut(s)	105, 389, 445, 995, 1578, 1701, 1964, 1973
HpyF3I	CTNAG	6 cut(s)	320, 795, 930, 1421, 1551, 1696
HpySE526I	ACGT	7 cut(s)	123, 237, 428, 1247, 1450, 1771, 1818
Hsp92I	GRCGYC	2 cut(s)	87, 186
HspAI	GCGC	3 cut(s)	381, 392, 1195
LmnI	GCTCC	4 cut(s)	355, 676, 1301, 1945
Lsp1109I	GCAGC	8 cut(s)	285, 315, 381, 435, 877, 985, 1294, 1404
LweI	GCATC	4 cut(s)	583, 708, 1224, 1720
MaeI	CTAG	2 cut(s)	872, 1262
MaeII	ACGT	7 cut(s)	123, 237, 428, 1247, 1450, 1771, 1818
MaeIII	GTNAC	4 cut(s)	500, 1168, 1240, 1521
MfeI	CAATTG	3 cut(s)	597, 1138, 1722
MflI	RGATCY	3 cut(s)	4, 1143, 2015
MhlI	GDGCHC	3 cut(s)	252, 360, 1832
MluI	ACGCGT	2 cut(s)	2091, 2109
MlyI	GAGTC	4 cut(s)	58, 312, 1089, 1915
MmeI	TCCRAC	1 cut(s)	411
Mph1103I	ATGCAT	1 cut(s)	701
MroXI	GAANNNNTTC	1 cut(s)	1560
MseI	TTAA	4 cut(s)	104, 785, 1253, 1410
MslI	CAYNNNNRTG	4 cut(s)	912, 1641, 1785, 1997
MspI	CCGG	7 cut(s)	1130, 1499, 1534, 1594, 1862, 1901, 1968
MspR9I	CCNGG	5 cut(s)	980, 1016, 1130, 1306, 1901
MunI	CAATTG	3 cut(s)	597, 1138, 1722
MvaI	CCWGG	3 cut(s)	980, 1016, 1306
MvnI	CGCG	4 cut(s)	381, 1629, 2093, 2111
MwoI	GCNNNNNNNGC	8 cut(s)	105, 389, 445, 995, 1578, 1701, 1964, 1973
NciI	CCSGG	2 cut(s)	1130, 1901
NlaIV	GGNNCC	4 cut(s)	209, 1303, 1432, 2017
NmeAIII	GCCGAG	2 cut(s)	63, 798
NmuCI	GTSAC	3 cut(s)	500, 1240, 1521
NruI	TCGCGA	1 cut(s)	1629
NsiI	ATGCAT	1 cut(s)	701
NspI	RCATGY	1 cut(s)	606
PaeI	GCATGC	1 cut(s)	606
PceI	AGGCCT	1 cut(s)	621
PcsI	WCGNNNNNNNCGW	3 cut(s)	96, 267, 313
PdmI	GAANNNNTTC	1 cut(s)	1560
PfeI	GAWTC	2 cut(s)	1709, 1890
PflMI	CCANNNNNTGG	1 cut(s)	1413
PfoI	TCCNGGA	2 cut(s)	1128, 1899
PkrI	GCNGC	9 cut(s)	300, 330, 396, 450, 892, 1000, 1284, 1419, 1860
Ple19I	CGATCG	2 cut(s)	711, 2043
PleI	GAGTC	4 cut(s)	58, 312, 1088, 1914
PmaCI	CACGTG	1 cut(s)	1248
PmlI	CACGTG	1 cut(s)	1248
PpsI	GAGTC	4 cut(s)	58, 312, 1088, 1914
Ppu21I	YACGTR	3 cut(s)	238, 1248, 1451
PsiI	TTATAA	1 cut(s)	720
Psp124BI	GAGCTC	1 cut(s)	360
Psp6I	CCWGG	3 cut(s)	978, 1014, 1304
PspCI	CACGTG	1 cut(s)	1248
PspEI	GGTNACC	1 cut(s)	1240
PspFI	CCCAGC	1 cut(s)	1433
PspGI	CCWGG	3 cut(s)	978, 1014, 1304
PspN4I	GGNNCC	4 cut(s)	209, 1303, 1432, 2017
PspPI	GGNCC	5 cut(s)	207, 972, 1430, 1570, 1580
PstI	CTGCAG	1 cut(s)	399
PsuI	RGATCY	3 cut(s)	4, 1143, 2015
PvuI	CGATCG	2 cut(s)	711, 2043
RruI	TCGCGA	1 cut(s)	1629
RseI	CAYNNNNRTG	4 cut(s)	912, 1641, 1785, 1997
SacI	GAGCTC	1 cut(s)	360
SalI	GTCGAC	2 cut(s)	306, 424
SaqAI	TTAA	4 cut(s)	104, 785, 1253, 1410
SatI	GCNGC	9 cut(s)	299, 329, 395, 449, 891, 999, 1283, 1418, 1859
Sau96I	GGNCC	5 cut(s)	207, 972, 1430, 1570, 1580
SchI	GAGTC	4 cut(s)	58, 312, 1089, 1915
ScrFI	CCNGG	5 cut(s)	980, 1016, 1130, 1306, 1901
SduI	GDGCHC	3 cut(s)	252, 360, 1832
SfaNI	GCATC	4 cut(s)	583, 708, 1224, 1720
SfcI	CTRYAG	3 cut(s)	395, 675, 1600
SfiI	GGCCNNNNNGGCC	1 cut(s)	1578
SinI	GGWCC	2 cut(s)	207, 1430
SmiMI	CAYNNNNRTG	4 cut(s)	912, 1641, 1785, 1997
SmlI	CTYRAG	1 cut(s)	2062
SmoI	CTYRAG	1 cut(s)	2062
SphI	GCATGC	1 cut(s)	606
SseBI	AGGCCT	1 cut(s)	621
SsiI	CCGC	6 cut(s)	345, 539, 822, 1859, 1942, 2105
SspMI	CTAG	2 cut(s)	872, 1262
SstI	GAGCTC	1 cut(s)	360
StuI	AGGCCT	1 cut(s)	621
StyD4I	CCNGG	5 cut(s)	978, 1014, 1128, 1304, 1899
StyI	CCWWGG	2 cut(s)	1426, 1438
TaaI	ACNGT	4 cut(s)	506, 1560, 1759, 1811
TaiI	ACGT	7 cut(s)	126, 240, 431, 1250, 1453, 1774, 1821
TaqI	TCGA	5 cut(s)	153, 307, 401, 425, 2043
TatI	WGTACW	4 cut(s)	291, 747, 774, 991
TauI	GCSGC	1 cut(s)	1861
TfiI	GAWTC	2 cut(s)	1709, 1890
Tru1I	TTAA	4 cut(s)	104, 785, 1253, 1410
Tru9I	TTAA	4 cut(s)	104, 785, 1253, 1410
TscAI	CASTG	4 cut(s)	1033, 1483, 1842, 2104
TseFI	GTSAC	3 cut(s)	500, 1240, 1521
TseI	GCWGC	8 cut(s)	298, 328, 394, 448, 890, 998, 1282, 1417
Tsp45I	GTSAC	3 cut(s)	500, 1240, 1521
TspDTI	ATGAA	5 cut(s)	1100, 1678, 1882, 2015, 2070
TspGWI	ACGGA	3 cut(s)	1445, 1967, 2064
TspRI	CASTG	4 cut(s)	1033, 1483, 1842, 2104
Van91I	CCANNNNNTGG	1 cut(s)	1413
VpaK11BI	GGWCC	2 cut(s)	207, 1430
XceI	RCATGY	1 cut(s)	606
XmiI	GTMKAC	3 cut(s)	307, 425, 2114
XmnI	GAANNNNTTC	1 cut(s)	1560
XspI	CTAG	2 cut(s)	872, 1262
Zsp2I	ATGCAT	1 cut(s)	701

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.