MD16G1115000.v1.1

Ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0024344
Physical Location & Seq
Reverse (-)
8156540 .. 8157154
615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1115000.v1.1.491

Sequence Viewer

Length: 615 bp
ATGCTATTGATTAGTGCCGGGTGCAATATCAACTCTCAGACAGAATCTGGAGAAACTGCACTAATGATCTGTGCTAGATATAAACACCAGGAATGCATTAAAATCCTGGCTTCAGACGGTGCTGATTTTGGCATGGTGAATGCTACTGGTCATAGTGCAAGCTTCATTGTAGACTCAGCAAGATGGGCTCTTAACTTCAGACAAGCAGTCGTAGACGTGATTCGATCTGGTGAAACTGTTCAATCAAGCGATACTTCAATATTTTCTCCTCGAATGCTTGTGACTCTAGCAAACGATGTTGATGCTTTGAAGAAACTGATTAAGGGAGCATACGTTAATCTCAATGAGCAGGATGAAAAGGGACACTCAGTTGCCATGATAGCTGTAGACGGTGGTTACCTTGAAACTTTCAAATTGCTTACCGACGCTGGGGCTGAAATGAACCTGCTGAATAAACATGGTCAGACTGCTATGGAACTCTTCGATACAAATCAATATGGCGAAGAATTTGAAAGATTATTGTTTAAGAATGCTGCAACACAAAAGGGACTCGATAGTCATGTTGAGTTTTATGGTACGCACGTCATGCAGCACAACACGGGAAAGTTGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4

Protein Analysis

205

Amino Acids

22.35

Weight (kDa)

5.31

Isoelectric Point (pI)

18.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 3 - 43 4.2e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 94 - 159 3e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024344)

Species Orthologous Gene IDs
malus_domestica MD16G1114400.v1.1 MD16G1115000.v1.1
pyrus_communis pycom234g00030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 555
Acc36I ACCTGC 1 cut(s) 453
AccI GTMKAC 3 cut(s) 171, 213, 387
AcsI RAATTY 1 cut(s) 506
AcuI CTGAAG 2 cut(s) 96, 181
AfaI GTAC 1 cut(s) 577
AfiI CCNNNNNNNGG 1 cut(s) 429
AgsI TTSAA 6 cut(s) 242, 258, 310, 404, 412, 512
AhdI GACNNNNNGTC 1 cut(s) 206
AjiI CACGTC 2 cut(s) 217, 583
AjnI CCWGG 2 cut(s) 87, 105
AluBI AGCT 2 cut(s) 162, 383
AluI AGCT 2 cut(s) 162, 383
AlwNI CAGNNNCTG 1 cut(s) 47
ApeKI GCWGC 2 cut(s) 533, 589
ApoI RAATTY 1 cut(s) 506
Asp700I GAANNNNTTC 1 cut(s) 237
AsuC2I CCSGG 1 cut(s) 19
AsuHPI GGTGA 2 cut(s) 148, 242
BaeI ACNNNNGTAYC 2 cut(s) 567, 600
BanII GRGCYC 1 cut(s) 190
BbvI GCAGC 2 cut(s) 520, 601
BccI CCATC 1 cut(s) 177
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BciT130I CCWGG 2 cut(s) 89, 107
BcnI CCSGG 1 cut(s) 19
BfaI CTAG 2 cut(s) 75, 287
BfmI CTRYAG 1 cut(s) 384
BfuAI ACCTGC 1 cut(s) 453
BisI GCNGC 2 cut(s) 534, 590
BlsI GCNGC 2 cut(s) 535, 591
Bme1390I CCNGG 3 cut(s) 19, 89, 107
BmeRI GACNNNNNGTC 1 cut(s) 206
BmgBI CACGTC 2 cut(s) 217, 583
BmrFI CCNGG 3 cut(s) 19, 89, 107
BmsI GCATC 1 cut(s) 292
BpmI CTGGAG 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 19
BsaBI GATNNNNATC 1 cut(s) 489
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 429
Bse1I ACTGG 1 cut(s) 151
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 2 cut(s) 89, 107
BseGI GGATG 1 cut(s) 358
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 1 cut(s) 429
BseMII CTCAG 3 cut(s) 50, 189, 381
BseNI ACTGG 1 cut(s) 151
BseRI GAGGAG 1 cut(s) 258
BseXI GCAGC 2 cut(s) 520, 601
BseYI CCCAGC 1 cut(s) 428
BsgI GTGCAG 1 cut(s) 42
BsiSI CCGG 1 cut(s) 18
BslFI GGGAC 2 cut(s) 375, 561
BslI CCNNNNNNNGG 1 cut(s) 429
BsmFI GGGAC 2 cut(s) 375, 561
BsmI GAATGC 4 cut(s) 98, 145, 279, 535
Bsp1286I GDGCHC 1 cut(s) 190
Bsp143I GATC 2 cut(s) 66, 224
BspCNI CTCAG 3 cut(s) 49, 188, 380
BspMI ACCTGC 1 cut(s) 453
BsrI ACTGG 1 cut(s) 151
BssMI GATC 2 cut(s) 66, 224
Bst2UI CCWGG 2 cut(s) 89, 107
Bst4CI ACNGT 3 cut(s) 119, 238, 392
Bst6I CTCTTC 1 cut(s) 485
BstAPI GCANNNNNTGC 1 cut(s) 586
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 3 cut(s) 36, 175, 367
BstEII GGTNACC 1 cut(s) 395
BstF5I GGATG 1 cut(s) 358
BstKTI GATC 2 cut(s) 69, 227
BstMBI GATC 2 cut(s) 66, 224
BstMWI GCNNNNNNNGC 3 cut(s) 185, 380, 586
BstNI CCWGG 2 cut(s) 89, 107
BstPI GGTNACC 1 cut(s) 395
BstSCI CCNGG 3 cut(s) 17, 87, 105
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 520, 601
BtrI CACGTC 2 cut(s) 217, 583
BtsCI GGATG 1 cut(s) 358
BveI ACCTGC 1 cut(s) 453
Cac8I GCNNGC 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 47
CseI GACGC 1 cut(s) 434
Csp6I GTAC 1 cut(s) 576
CviAII CATG 5 cut(s) 133, 376, 458, 560, 586
CviJI RGCY 5 cut(s) 110, 162, 188, 383, 434
CviKI_1 RGCY 5 cut(s) 110, 162, 188, 383, 434
CviQI GTAC 1 cut(s) 576
DdeI CTNAG 3 cut(s) 36, 175, 367
DpnI GATC 2 cut(s) 68, 226
DpnII GATC 2 cut(s) 66, 224
DrdI GACNNNNNNGTC 1 cut(s) 555
DriI GACNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 555
Eam1104I CTCTTC 1 cut(s) 485
Eam1105I GACNNNNNGTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 485
Eco24I GRGCYC 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 96, 181
Eco91I GGTNACC 1 cut(s) 395
EcoO65I GGTNACC 1 cut(s) 395
EcoRII CCWGG 2 cut(s) 87, 105
EcoT22I ATGCAT 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 190
FaeI CATG 5 cut(s) 136, 379, 461, 563, 589
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 2 cut(s) 375, 561
FatI CATG 5 cut(s) 132, 375, 457, 559, 585
FblI GTMKAC 3 cut(s) 171, 213, 387
Fnu4HI GCNGC 2 cut(s) 534, 590
FokI GGATG 1 cut(s) 365
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 2 cut(s) 534, 590
FspBI CTAG 2 cut(s) 75, 287
GluI GCNGC 2 cut(s) 534, 590
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 1 cut(s) 69
HapII CCGG 1 cut(s) 18
HgaI GACGC 1 cut(s) 434
Hin1II CATG 5 cut(s) 136, 379, 461, 563, 589
HindIII AAGCTT 1 cut(s) 160
HinfI GANTC 5 cut(s) 44, 173, 220, 283, 549
HpaII CCGG 1 cut(s) 18
HphI GGTGA 2 cut(s) 148, 242
Hpy166II GTNNAC 3 cut(s) 172, 214, 388
Hpy188I TCNGA 4 cut(s) 39, 115, 200, 465
Hpy188III TCNNGA 1 cut(s) 48
Hpy8I GTNNAC 3 cut(s) 172, 214, 388
Hpy99I CGWCG 1 cut(s) 428
HpyCH4III ACNGT 3 cut(s) 119, 238, 392
HpyCH4IV ACGT 3 cut(s) 216, 333, 582
HpyCH4V TGCA 6 cut(s) 24, 59, 96, 158, 536, 589
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 380, 586
HpyF3I CTNAG 3 cut(s) 36, 175, 367
HpySE526I ACGT 3 cut(s) 216, 333, 582
Hsp92II CATG 5 cut(s) 136, 379, 461, 563, 589
Kzo9I GATC 2 cut(s) 66, 224
LmnI GCTCC 1 cut(s) 326
Lsp1109I GCAGC 2 cut(s) 520, 601
LweI GCATC 1 cut(s) 292
MaeI CTAG 2 cut(s) 75, 287
MaeII ACGT 3 cut(s) 216, 333, 582
MaeIII GTNAC 2 cut(s) 280, 395
MalI GATC 2 cut(s) 68, 226
MboI GATC 2 cut(s) 66, 224
MboII GAAGA 3 cut(s) 322, 472, 515
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 2 cut(s) 413, 506
MlyI GAGTC 3 cut(s) 167, 277, 543
MnlI CCTC 1 cut(s) 279
Mph1103I ATGCAT 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 5 cut(s) 99, 192, 321, 336, 525
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 3 cut(s) 19, 89, 107
Mva1269I GAATGC 4 cut(s) 98, 145, 279, 535
MvaI CCWGG 2 cut(s) 89, 107
MwoI GCNNNNNNNGC 3 cut(s) 185, 380, 586
NciI CCSGG 1 cut(s) 19
NdeII GATC 2 cut(s) 66, 224
NlaIII CATG 5 cut(s) 136, 379, 461, 563, 589
NmuCI GTSAC 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 98
PctI GAATGC 4 cut(s) 98, 145, 279, 535
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 2 cut(s) 44, 220
PkrI GCNGC 2 cut(s) 535, 591
PleI GAGTC 3 cut(s) 167, 277, 543
PpsI GAGTC 3 cut(s) 167, 277, 543
Psp6I CCWGG 2 cut(s) 87, 105
PspEI GGTNACC 1 cut(s) 395
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 2 cut(s) 87, 105
PstNI CAGNNNCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 577
RsaNI GTAC 1 cut(s) 576
SaqAI TTAA 5 cut(s) 99, 192, 321, 336, 525
SatI GCNGC 2 cut(s) 534, 590
Sau3AI GATC 2 cut(s) 66, 224
SchI GAGTC 3 cut(s) 167, 277, 543
ScrFI CCNGG 3 cut(s) 19, 89, 107
SduI GDGCHC 1 cut(s) 190
SetI ASST 7 cut(s) 164, 219, 336, 385, 402, 447, 585
SfaNI GCATC 1 cut(s) 292
SfcI CTRYAG 1 cut(s) 384
Sse9I AATT 2 cut(s) 413, 506
SspI AATATT 1 cut(s) 261
SspMI CTAG 2 cut(s) 75, 287
StyD4I CCNGG 3 cut(s) 17, 87, 105
TaaI ACNGT 3 cut(s) 119, 238, 392
TaiI ACGT 3 cut(s) 219, 336, 585
TaqI TCGA 4 cut(s) 223, 271, 483, 552
TasI AATT 2 cut(s) 413, 506
TfiI GAWTC 2 cut(s) 44, 220
Tru1I TTAA 5 cut(s) 99, 192, 321, 336, 525
Tru9I TTAA 5 cut(s) 99, 192, 321, 336, 525
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 2 cut(s) 533, 589
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 3 cut(s) 154, 369, 455
XapI RAATTY 1 cut(s) 506
XmiI GTMKAC 3 cut(s) 171, 213, 387
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 2 cut(s) 75, 287
Zsp2I ATGCAT 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.