pycom234g00030

Ankyrin repeats (3 copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000234
OG0024344
Physical Location & Seq
Forward (+)
25295 .. 26166
872 bp
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UTR
Exon/CDS
Intron
pycom234g00030.1

Sequence Viewer

Length: 402 bp
ATGACAGTGATGCCATTTATTAGTGCCGGGTGCAATATCAACTCTTCGACAGAATCTGGAGAAACTACACTAATGATCTGTGCTAGATATAAACACCAGGAATGCCTTAAAATTCTGGCTTCAGACGGTGCTGATTTTGGCCTGGTGAATGCTACTGGTCATAGTGCAAGCTTATTGTCATGTGCTCTTGATGCCGATGGATACACTACGTTGATGTTAGCAGCAACAGGAGGTCACGCTACGCTGTGCGAGCTTTTGATTTCTTTAGGATCTAGATGCGACAATACTAATGCAAGACACGAGAGCTCTCGCTTGCTAGGAAAAGTGGGATTAAAAATGATGCAGAGAACGTGCTATTGTACGAGCTTGCAAGAAGCCTTGTTCTTGGTGGGAGGCACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4

Protein Analysis

134

Amino Acids

14.03

Weight (kDa)

6.03

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 6 - 96 2.9e-11 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024344)

Species Orthologous Gene IDs
malus_domestica MD16G1114400.v1.1 MD16G1115000.v1.1
pyrus_communis pycom234g00030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 1 cut(s) 111
AcuI CTGAAG 1 cut(s) 105
AfaI GTAC 1 cut(s) 361
AjnI CCWGG 2 cut(s) 96, 141
AluBI AGCT 4 cut(s) 171, 253, 306, 366
AluI AGCT 4 cut(s) 171, 253, 306, 366
Alw21I GWGCWC 2 cut(s) 187, 308
AlwI GGATC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 56
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 221
ApoI RAATTY 1 cut(s) 111
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 157
BanII GRGCYC 1 cut(s) 308
BauI CACGAG 1 cut(s) 299
Bbv12I GWGCWC 2 cut(s) 187, 308
BbvI GCAGC 1 cut(s) 233
BccI CCATC 1 cut(s) 191
BciT130I CCWGG 2 cut(s) 98, 143
BciVI GTATCC 1 cut(s) 194
BcnI CCSGG 1 cut(s) 28
BfaI CTAG 3 cut(s) 84, 273, 317
BfuI GTATCC 1 cut(s) 194
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 3 cut(s) 28, 98, 143
BmrFI CCNGG 3 cut(s) 28, 98, 143
BmsI GCATC 3 cut(s) 181, 266, 330
BpmI CTGGAG 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 28
BsaAI YACGTR 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 160
BseBI CCWGG 2 cut(s) 98, 143
BseNI ACTGG 1 cut(s) 160
BseXI GCAGC 1 cut(s) 233
BshFI GGCC 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 187, 308
BsiSI CCGG 1 cut(s) 27
BsmI GAATGC 2 cut(s) 107, 154
BsnI GGCC 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 187, 308
Bsp143I GATC 2 cut(s) 75, 269
BspANI GGCC 1 cut(s) 141
BspPI GGATC 1 cut(s) 277
BsrI ACTGG 1 cut(s) 160
BssMI GATC 2 cut(s) 75, 269
BssSI CACGAG 1 cut(s) 299
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 2 cut(s) 98, 143
Bst4CI ACNGT 2 cut(s) 7, 128
Bst6I CTCTTC 1 cut(s) 49
BstBAI YACGTR 1 cut(s) 399
BstC8I GCNNGC 4 cut(s) 169, 251, 314, 368
BstKTI GATC 2 cut(s) 78, 272
BstMBI GATC 2 cut(s) 75, 269
BstMWI GCNNNNNNNGC 2 cut(s) 191, 250
BstNI CCWGG 2 cut(s) 98, 143
BstSCI CCNGG 3 cut(s) 26, 96, 141
BstV1I GCAGC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 4 cut(s) 169, 251, 314, 368
CaiI CAGNNNCTG 1 cut(s) 56
Csp6I GTAC 1 cut(s) 360
CviAII CATG 1 cut(s) 180
CviJI RGCY 7 cut(s) 119, 141, 171, 253, 306, 366, 377
CviKI_1 RGCY 7 cut(s) 119, 141, 171, 253, 306, 366, 377
CviQI GTAC 1 cut(s) 360
DpnI GATC 2 cut(s) 77, 271
DpnII GATC 2 cut(s) 75, 269
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 308
Eco53kI GAGCTC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 105
EcoICRI GAGCTC 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 96, 141
EcoT38I GRGCYC 1 cut(s) 308
FaeI CATG 1 cut(s) 183
FaiI YATR 3 cut(s) 90, 162, 181
FatI CATG 1 cut(s) 179
Fnu4HI GCNGC 1 cut(s) 222
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 3 cut(s) 84, 273, 317
GluI GCNGC 1 cut(s) 222
GsuI CTGGAG 1 cut(s) 78
HaeIII GGCC 1 cut(s) 141
HapII CCGG 1 cut(s) 27
Hin1II CATG 1 cut(s) 183
HindIII AAGCTT 1 cut(s) 169
HinfI GANTC 1 cut(s) 53
HpaII CCGG 1 cut(s) 27
HphI GGTGA 1 cut(s) 157
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 3 cut(s) 57, 188, 273
HpyCH4III ACNGT 2 cut(s) 7, 128
HpyCH4IV ACGT 3 cut(s) 209, 350, 398
HpyCH4V TGCA 5 cut(s) 33, 167, 293, 343, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 250
HpySE526I ACGT 3 cut(s) 209, 350, 398
Hsp92II CATG 1 cut(s) 183
Kzo9I GATC 2 cut(s) 75, 269
LpnPI CCDG 9 cut(s) 40, 42, 83, 101, 110, 128, 141, 155, 213
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 3 cut(s) 181, 266, 330
MaeI CTAG 3 cut(s) 84, 273, 317
MaeII ACGT 3 cut(s) 209, 350, 398
MaeIII GTNAC 1 cut(s) 233
MalI GATC 2 cut(s) 77, 271
MboI GATC 2 cut(s) 75, 269
MboII GAAGA 1 cut(s) 36
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 2 cut(s) 187, 308
MluCI AATT 1 cut(s) 111
MnlI CCTC 2 cut(s) 224, 386
MseI TTAA 2 cut(s) 108, 332
MspI CCGG 1 cut(s) 27
MspR9I CCNGG 3 cut(s) 28, 98, 143
Mva1269I GAATGC 2 cut(s) 107, 154
MvaI CCWGG 2 cut(s) 98, 143
MwoI GCNNNNNNNGC 2 cut(s) 191, 250
NciI CCSGG 1 cut(s) 28
NdeII GATC 2 cut(s) 75, 269
NlaIII CATG 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 233
PctI GAATGC 2 cut(s) 107, 154
PfeI GAWTC 1 cut(s) 53
PkrI GCNGC 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 308
Psp6I CCWGG 2 cut(s) 96, 141
PspGI CCWGG 2 cut(s) 96, 141
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 269
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SacI GAGCTC 1 cut(s) 308
SaqAI TTAA 2 cut(s) 108, 332
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 2 cut(s) 75, 269
ScrFI CCNGG 3 cut(s) 28, 98, 143
SduI GDGCHC 2 cut(s) 187, 308
SetI ASST 8 cut(s) 173, 212, 235, 255, 308, 353, 368, 401
SfaNI GCATC 3 cut(s) 181, 266, 330
Sse9I AATT 1 cut(s) 111
SspMI CTAG 3 cut(s) 84, 273, 317
SstI GAGCTC 1 cut(s) 308
StyD4I CCNGG 3 cut(s) 26, 96, 141
TaaI ACNGT 2 cut(s) 7, 128
TaiI ACGT 3 cut(s) 212, 353, 401
TaqI TCGA 1 cut(s) 47
TasI AATT 1 cut(s) 111
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 2 cut(s) 108, 332
Tru9I TTAA 2 cut(s) 108, 332
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 233
TspRI CASTG 1 cut(s) 12
XapI RAATTY 1 cut(s) 111
XbaI TCTAGA 1 cut(s) 272
XspI CTAG 3 cut(s) 84, 273, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.