Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005952

Physical Location & Seq

Genomic Coordinates Forward (+)

23848686 .. 23864211

Gene Structure 15526 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1232500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4527 bp

ATGGCTGCCAATGTCAGTTTGGTGGTTGGATCTCATGTTTGGGTCGAGGATCCTGAGGAAGCCTGGATTGATGGAGAAGTTGTAGGAGTCAATAATGAAGAGATTGAAATAAACTGTACATCAGGGAAGACGGTTACGGTCACAGCATCTAGTGTATACCCAAAGGATCCTGAATTTCCTCAATGTGGTGTAGACGATATGACAAAGTTGGCTTATCTACATGAACCAGGGGTTTTGCAGAATTTAAAAAGTCGATACACTATAAATGAAATATATACTTATACAGGAAGTATATTGATAGCTGTGAACCCTTTCCGGAGGTTACCTCATTTATATGATAATCATATGATGGAGCAGTATAAAGGGGTGACTTTAGGTGAGCTGAGTCCACATCCTTTTGCCATTGCTGATGCTGCCTACAGACAGATGATTAACGAGGGAATAAGCCAGGCAATTTTGGTCAGTGGGGAAAGTGGAGCCGGAAAAACAGAAAGTACAAAGATGCTTATGCGTTATCTTGCCCATATGGGTGGAAGAGCTGTATCTGAGGGGCGGTCTGTGGAACAGCAAGTCCTGGAGTCTAACCCTGTTCTCGAAGCATTTGGTAATGCAAAGACTGTCAGAAATAATAATTCCAGTCGTTTTGGTAAGTTCGTGGAGCTTCAGTTTGATAAAACGGGGAGAATCTCAGGAGCTGCCATTAGAACTTATTTATTGGAGCGATCCCGAGTTTGTCAAGTATCTGATCCAGAGAGAAATTACCATTGCTTTTATATGCTCTGTTCTGCACCTCCAGAGGATGTTGAGAAGTACAAATTGGGAAATCTAAGAACATTTCATTATTTAAATCAGTCTAACTGCTATGAGCTGGACGGGGTGGATGATTCTAAGGAGTACCTCACAACTAGGAAAGCTATGGATGTGGTTGGGATCAGCTCTGATGAGCAGGATGCAATATTTCGTGTTGTAGCTGCAATACTTCATTTAGGCAATGTTAACTTTGCAAAGGAGAATGAAACTGATTCTTCTGAACCCAAGGATGATAAATCTCGGTTCCATCTCAAAACTGCTGCTGAACTTTTCATGTGTGATGAACAATCTCTTGAAGATTCTTTGTGCAAACGTGTGATTGTGACCCGTGATGAGACCATAACGAAAAGCTTGGATCCAGATTCTGCAGCCATCAGTAGAGACGCATTGGCCAAAATTGTTTATTCAAGATTATTTGATTGGCTTGTGAACAAGATAAATAACTCTATTGGTCAGGATCCTGATTCCAAAAATTTGATTGGAGTTCTGGATATTTATGGATTCGAGAGTTTCAAGACAAACAGCTTCGAGCAATTTTGTATCAATTTGACAAATGAAAAATTGCAGCAGCATTTCAACCAGCATGTGTTCAAGATGGAGCAAGAAGAATATACTAAAGAAGAAATTGATTGGAGTTACATAGAGTTCATCGATAATCAGGATATTCTTGATCTCATTGAAAAGAAGCCTGGTGGCATCATTGCTCTTCTGGATGAGGCTTGTATGTTCCCAAGATCAACACATGAAACATTTTCACAGAAGCTTTATCAAACTTTCAAAGACCATAAACGTTTTAACAAGCCGAAATTGTCGCCTACCGACTTTACCATTTGCCATTATGCTGGTGATGTTACTTATCAAACAGAGTTCTTCCTGGATAAGAACAAAGATTATGTTGTTGCAGAGCATCAAGCACTCCTTAGTGCCTCCAAATGCTCTTTTGTTTCAGGCTTGTTCCCTCTTTTACCTGAAGAATCTGCCAAACAATCAAAGTTCTCCTCAATAGGTTCTCAATTCAAGCAACAACTGCAATCACTGCTTGAAACGCTAAGTGCCATTGAGCCTCACTACATACGCTGTGTAAAGCCAAATAATCAACTCAAACCGGCCATATTTGAAAATAGTGCTGTTTTACAGCAGCTTTGCTGCGGGGGAGTAATGGAGGCAATTCGGATTAGTTGTGCTGGATATCCAACTAGAAAGACATTTGCAGAATTTACAAGTCGTTTTAAAGTTCTGGCACCTGAGGTTCTAAATGGAAGCGTTGATGAGGTCACTGCATGCAAGAAGCTTTTACAAAAGGTCAACCTCAAGGGATATCAGGTTGGGAAAACAAAAGTGTTCCTCAGGGCTGGCCAGATGGCAGAATTAGATGCTTATCGAAGTGAGGTATTGGGAAGATCGGCTAGCATTATGCAGAGAAAAGTTCGCTCCTACCTATGTCGCAAAAAATTTGTCCTGTTGCAGTTGTCTGCCATCCAAGTCCAAGCCTTATGTAGAGGACAAGTTGCACGCCGTCGATATGAAAACATGAGAAGGGAAGCTGGTTCTGTGATTATCCAGAAATATGGTCGCTCGTATCTTTCTAGGAATGGTTATAAAAACTTGTGCTCTTCAGCTGTTTCTATTCAAACTGGTATGCGAGGGCTGGCTGCTCGTAAGGAGCTTAGGTCCAGGAGGAAAACAAGGGCTGCAACTGTGATACAGAGTCATTGCCGGAAACACTTAGCCCATGCTCGTTATTTGAGGATGAAGAAAGCAGCTATTGTGTGGCAATGTGGTTGGAGACAAATAGTTGCTCGTAAAGAGTTGCGGAAGCTAAAGATGGCTGCAAAGGAAACTGGTGCTCTCCAAGAAGCCAAAAATAAGCTGGAAAAGGAAGTTGAAGAGCTAAGCTGGCGCCTGCAGCTGGAGAAGCAAATGAGGGCTGACATGGAAGAGGCCAAAACAAAGGAAAATGCGAAGTTGCAGTCTGCTTTGAAAGAGATGCAACTTGAATTTCAAGAAACTAAATCCCTGCTTATGAAGGAACGTGAAGCTGCAGCAACAGCATCAGAGGAGGCAGCAAAAGCGGCTGAGGCTGCAGCAAAAGTAGCTGAACAAGTGCCAGTTGTACAGGAGGTTTCAGTTGTTGATCAGGAAATAGTCAATAAGCTTACTGCTGAAAATGAGCAGCTCAAGGCCCTCGTAGACTCACTGGAAAAGAAGATTGATGAAACAAAGAAAAAGTTTGAAGAAACAAGCAGGCTCAGTGAAGAGCGGCTCAAGCAGGCTACGGAGGCAGAATCGAAGATAATTGATTTGAAGACTGCCATGCAGAGGCTAGAAGAAAAACTTTCTGACATAGAAACCGAGGACCAAATTTTGCGTCAGCAAGCATTAAAGATGTCAGCTTCTATGAAAATGTCAGAGCATTTGGCAGGAATAGAAACTCAGCCTTTGGGGAATGGTCATCATGAACCACTGAGACCTACACCGTCAAAGAAATTTGGCTCAGAGTCTTTCAGGAAATCCCAAATTGAAAGACAACATGAGGGTGTAGATACTCTTATGAAATGCGTGACAGGTAATCTTGGGTTCAGTGAAGGAAAGCCAGTGGCCGCAATAACACTATACAAATGTCTTCTCCACTGGAAATTATTTGAAGCGGAAAAAACTAGTGTATTTGACCGACTTATTCAGATAATTGGTTCTGAAATTGAGGACCAGGATAACAATGATCATATGGCTTATTGGCTTTCCAATACATCTACATTGTTGTTCTTGCTCCAGCGGAGTTTAAGAACTGCTCCACGAAAACCTCCTACTCCTACATCTTTATTTGGAAGGATGACCCAAGGCTTCCGATCGTCTTCTACCAATCTTTCAGTTGGTGCTTCTGATTTAGTGCGTCAGGTTGAGGCCAAGTATCCGGCTTTGCTCTTTAAGCAGCAACTTACAGCATACGTAGAAAAGATATATGGAATCGTCCGAGACAATTTGAAGAAGGAATTATCACTTCATCTTTCTTCTTGCATCCAGGCACCCAAAACAGCAAAAAGTGGTGTTTCAAAATCACCTGAAGGATCAACTGGGAACAGTCCTCAGGCTAATCCTTGGAATAGCATCATTGCAAATCTTAGTGGGTTTCTCTCTACATTGAACGAAAATTGTGTGCCTCCAATTCTTGTCCAGAAGATATTTACTCAAATGTTCTCATATATCAATGTACAGATTTTTAATAGCCTTCTTCTTCGTCAAGAGTGCTGCACCTTCAACAATGGCCAATATGTGAAGAATGGGCTAGCTGAATTGGAGCTATGGTGTAACCTAGCAAAAGAAGAGTATGCTGGCTCATCATGGGATGAACTCAAATATATTAGGCAGGCTGTAGGATTCTTGGTTCTACACCAAAAGTCCAGAATCTCCTATGATGAACTAACAAATGACCTATGCCCTGCATTGAGTGTTCAACAACTTCATAGAGTATGCACACTATTTTTGGATGACGATTACAACTCTCAAAGTGTTGCCCCTGATGTCATTTCAAGCATGAACTTTCTAATGACAGATGACTCCAGCGGTGAAGATAGCAGCTCCTTTTTGCTGGATGACAATTCCAGCCTTCCTTTCTCAATTGAAGATATATCTGGTTCCATGCAAGACCAGAGCTATTCAGATGTAAAACCTCCTGCAGAGCTTTTGGAGAATCCAGACTTCCACTTTTTACAGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000146 GO:0000166 GO:0000331 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005856 GO:0006928 GO:0008092 GO:0008144 GO:0008150 GO:0009987 GO:0015629 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0030029 GO:0030048 GO:0030554 GO:0030898 GO:0031410 GO:0031982 GO:0032553 GO:0032555 GO:0032559 GO:0033275 GO:0035639 GO:0036094 GO:0042623 GO:0042641 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043531 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0051015 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051656 GO:0070252 GO:0097159 GO:0097367 GO:0097708 GO:0140027 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DIL IQ Myosin_N Myosin_head

Protein Analysis

1509

Amino Acids

170.28

Weight (kDa)

6.44

Isoelectric Point (pI)

45.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myosin_N	PF02736	9 - 52	3e-13	Myosin N-terminal SH3-like domain
Myosin_head	PF00063	64 - 720	1.3e-257	Myosin head (motor domain)
DIL	PF01843	1329 - 1433	1e-26	DIL domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005952)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04600 AT2G33240 AT2G33240 AT2G33240 AT2G33240 AT2G33240 AT2G33240 AT2G33240 AT2G33240
fragaria_vesca	FvH4_4g05270 FvH4_4g05270
malus_domestica	MD13G1226900.v1.1 MD16G1232500.v1.1
prunus_persica	Prupe.1G054200_v2.0.a1 Prupe.1G054200_v2.0.a1
pyrus_communis	pycom13g19990 pycom16g19440
rosa_chinensis	RchiOBHm_Chr4g0396201
rosa_laevigata	RLG00000009559
rosa_multiflora	Rmu_sc0006325.1_g000047
rosa_roxburghii	Rroxscaffold_5G00341050
rosa_rugosa	Rorug03G0350800 Rorug03G0350800
rosa_samantha	Rh4BG065700 Rh4CG072400 Rh4DG062500
rosa_wichuraiana	Rw4G005420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2409
AccB1I	GGYRCC	3 cut(s)	2050, 2709, 3832
AccBSI	CCGCTC	1 cut(s)	3070
AccI	GTMKAC	3 cut(s)	156, 192, 3000
AccIII	TCCGGA	1 cut(s)	315
AciI	CCGC	9 cut(s)	553, 1959, 2623, 2882, 3070, 3411, 3458, 3583, 4371
AclI	AACGTT	1 cut(s)	1600
AcoI	YGGCCR	5 cut(s)	1200, 1917, 2164, 3408, 4072
AcsI	RAATTY	8 cut(s)	173, 241, 1282, 2024, 2261, 2807, 3171, 3296
AcuI	CTGAAG	4 cut(s)	647, 1800, 2409, 3891
AcyI	GRCGYC	1 cut(s)	2710
AfaI	GTAC	6 cut(s)	118, 496, 812, 896, 2925, 4020
AfiI	CCNNNNNNNGG	3 cut(s)	185, 2719, 3129
AflIII	ACRYGT	1 cut(s)	1123
AhlI	ACTAGT	1 cut(s)	3467
AjnI	CCWGG	9 cut(s)	62, 226, 447, 573, 1498, 1683, 2483, 3516, 3828
AjuI	GAANNNNNNNTTGG	2 cut(s)	800, 832
AloI	GAACNNNNNNTCC	4 cut(s)	555, 587, 4176, 4208
Alw21I	GWGCWC	2 cut(s)	2423, 2659
Alw26I	GTCTC	5 cut(s)	1139, 1185, 2590, 3271, 3777
AlwNI	CAGNNNCTG	2 cut(s)	695, 1175
Ama87I	CYCGRG	1 cut(s)	726
Aor13HI	TCCGGA	1 cut(s)	315
AoxI	GGCC	8 cut(s)	1200, 1917, 2164, 2751, 2991, 3408, 3711, 4072
ApoI	RAATTY	8 cut(s)	173, 241, 1282, 2024, 2261, 2807, 3171, 3296
ArsI	GACNNNNNNTTYG	6 cut(s)	2250, 2282, 2765, 2797, 3163, 3195
Asp700I	GAANNNNTTC	1 cut(s)	311
AspLEI	GCGC	1 cut(s)	2712
AspS9I	GGNCC	4 cut(s)	2481, 2992, 3166, 3514
AsuHPI	GGTGA	5 cut(s)	379, 389, 1667, 3858, 4385
AsuNHI	GCTAGC	2 cut(s)	2216, 4093
AvaI	CYCGRG	1 cut(s)	726
AvaII	GGWCC	3 cut(s)	2481, 3166, 3514
AxyI	CCTNAGG	4 cut(s)	54, 2055, 2156, 3894
BalI	TGGCCA	3 cut(s)	1202, 2166, 4074
BamHI	GGATCC	4 cut(s)	49, 166, 1165, 1267
BanI	GGYRCC	3 cut(s)	2050, 2709, 3832
BbsI	GAAGAC	4 cut(s)	134, 3122, 3425, 3654
Bbv12I	GWGCWC	2 cut(s)	2423, 2659
BbvCI	CCTCAGC	1 cut(s)	2886
BccI	CCATC	8 cut(s)	65, 343, 1065, 1190, 1399, 2164, 2294, 2629
BceAI	ACGGC	1 cut(s)	2310
BciT130I	CCWGG	9 cut(s)	64, 228, 449, 575, 1500, 1685, 2485, 3518, 3830
BciVI	GTATCC	1 cut(s)	3729
BclI	TGATCA	2 cut(s)	2944, 3529
BcoDI	GTCTC	5 cut(s)	1139, 1185, 2590, 3271, 3777
BcuI	ACTAGT	1 cut(s)	3467
BfaI	CTAG	9 cut(s)	150, 906, 2007, 2217, 2397, 3134, 3468, 4094, 4121
BfmI	CTRYAG	7 cut(s)	418, 1176, 2714, 2850, 2892, 4179, 4482
BfoI	RGCGCY	1 cut(s)	2713
BfuI	GTATCC	1 cut(s)	3729
BlpI	GCTNAGC	1 cut(s)	2702
Bme1390I	CCNGG	9 cut(s)	64, 228, 449, 575, 1500, 1685, 2485, 3518, 3830
Bme18I	GGWCC	3 cut(s)	2481, 3166, 3514
BmeT110I	CYCGRG	1 cut(s)	726
BmgT120I	GGNCC	4 cut(s)	2481, 2992, 3166, 3514
BmrFI	CCNGG	9 cut(s)	64, 228, 449, 575, 1500, 1685, 2485, 3518, 3830
BmrI	ACTGGG	1 cut(s)	3891
BmtI	GCTAGC	2 cut(s)	2220, 4097
BmuI	ACTGGG	1 cut(s)	3891
BpiI	GAAGAC	4 cut(s)	134, 3122, 3425, 3654
BplI	GAGNNNNNCTC	2 cut(s)	310, 342
BpmI	CTGGAG	5 cut(s)	596, 777, 2741, 3563, 4351
Bpu10I	CCTNAGC	2 cut(s)	2477, 2886
Bpu1102I	GCTNAGC	1 cut(s)	2702
BpuEI	CTTGAG	3 cut(s)	2105, 2972, 3059
Bsa29I	ATCGAT	1 cut(s)	1461
BsaAI	YACGTR	1 cut(s)	3757
BsaBI	GATNNNNATC	1 cut(s)	2187
BsaHI	GRCGYC	1 cut(s)	2710
BsaI	GGTCTC	2 cut(s)	1139, 3271
BsaJI	CCNNGG	5 cut(s)	227, 1035, 3162, 3646, 3905
BsaWI	WCCGGW	1 cut(s)	315
BsaXI	ACNNNNNCTCC	8 cut(s)	66, 96, 650, 680, 2465, 2495, 4097, 4127
Bsc4I	CCNNNNNNNGG	3 cut(s)	185, 2719, 3129
Bse118I	RCCGGY	1 cut(s)	1915
Bse1I	ACTGG	8 cut(s)	636, 2449, 2656, 2918, 3012, 3404, 3446, 3886
Bse21I	CCTNAGG	4 cut(s)	54, 2055, 2156, 3894
Bse3DI	GCAATG	7 cut(s)	402, 763, 997, 1509, 2521, 2591, 3918
Bse8I	GATNNNNATC	1 cut(s)	2187
BseAI	TCCGGA	1 cut(s)	315
BseBI	CCWGG	9 cut(s)	64, 228, 449, 575, 1500, 1685, 2485, 3518, 3830
BseCI	ATCGAT	1 cut(s)	1461
BseDI	CCNNGG	5 cut(s)	227, 1035, 3162, 3646, 3905
BseJI	GATNNNNATC	1 cut(s)	2187
BseLI	CCNNNNNNNGG	3 cut(s)	185, 2719, 3129
BseMI	GCAATG	7 cut(s)	402, 763, 997, 1509, 2521, 2591, 3918
BseNI	ACTGG	8 cut(s)	636, 2449, 2656, 2918, 3012, 3404, 3446, 3886
BseRI	GAGGAG	2 cut(s)	1798, 2882
BsgI	GTGCAG	2 cut(s)	771, 4042
Bsh1285I	CGRYCG	1 cut(s)	3659
BshFI	GGCC	8 cut(s)	1202, 1919, 2166, 2753, 2993, 3410, 3713, 4074
BshNI	GGYRCC	3 cut(s)	2050, 2709, 3832
BshVI	ATCGAT	1 cut(s)	1461
BsiEI	CGRYCG	1 cut(s)	3659
BsiHKAI	GWGCWC	2 cut(s)	2423, 2659
BsiHKCI	CYCGRG	1 cut(s)	726
BsiSI	CCGG	5 cut(s)	316, 480, 1916, 2527, 3722
BslI	CCNNNNNNNGG	3 cut(s)	185, 2719, 3129
BsmAI	GTCTC	5 cut(s)	1139, 1185, 2590, 3271, 3777
BsmBI	CGTCTC	1 cut(s)	1185
BsnI	GGCC	8 cut(s)	1202, 1919, 2166, 2753, 2993, 3410, 3713, 4074
Bso31I	GGTCTC	2 cut(s)	1139, 3271
BsoBI	CYCGRG	1 cut(s)	726
Bsp1286I	GDGCHC	2 cut(s)	2423, 2659
Bsp13I	TCCGGA	1 cut(s)	315
Bsp1407I	TGTACA	3 cut(s)	116, 2923, 4018
Bsp1720I	GCTNAGC	1 cut(s)	2702
BspACI	CCGC	9 cut(s)	553, 1959, 2623, 2882, 3070, 3411, 3458, 3583, 4371
BspANI	GGCC	8 cut(s)	1202, 1919, 2166, 2753, 2993, 3410, 3713, 4074
BspDI	ATCGAT	1 cut(s)	1461
BspEI	TCCGGA	1 cut(s)	315
BspHI	TCATGA	1 cut(s)	3265
BspMAI	CTGCAG	5 cut(s)	1180, 2718, 2854, 2896, 4486
BspOI	GCTAGC	2 cut(s)	2220, 4097
BspQI	GCTCTTC	5 cut(s)	529, 1521, 2428, 2691, 3060
BspT107I	GGYRCC	3 cut(s)	2050, 2709, 3832
BspTNI	GGTCTC	2 cut(s)	1139, 3271
BsrBI	CCGCTC	1 cut(s)	3070
BsrDI	GCAATG	7 cut(s)	402, 763, 997, 1509, 2521, 2591, 3918
BsrFI	RCCGGY	1 cut(s)	1915
BsrGI	TGTACA	3 cut(s)	116, 2923, 4018
BsrI	ACTGG	8 cut(s)	636, 2449, 2656, 2918, 3012, 3404, 3446, 3886
BssAI	RCCGGY	1 cut(s)	1915
BssECI	CCNNGG	5 cut(s)	227, 1035, 3162, 3646, 3905
BssNAI	GTATAC	1 cut(s)	157
BssNI	GRCGYC	1 cut(s)	2710
BssT1I	CCWWGG	3 cut(s)	1035, 3646, 3905
Bst1107I	GTATAC	1 cut(s)	157
Bst2UI	CCWGG	9 cut(s)	64, 228, 449, 575, 1500, 1685, 2485, 3518, 3830
Bst4CI	ACNGT	7 cut(s)	116, 133, 139, 619, 2509, 3288, 3890
Bst6I	CTCTTC	8 cut(s)	93, 529, 1521, 2428, 2691, 2742, 3060, 4125
BstACI	GRCGYC	1 cut(s)	2710
BstAPI	GCANNNNNTGC	2 cut(s)	1837, 1846
BstAUI	TGTACA	3 cut(s)	116, 2923, 4018
BstBAI	YACGTR	1 cut(s)	3757
BstEII	GGTNACC	1 cut(s)	321
BstH2I	RGCGCY	1 cut(s)	2713
BstHHI	GCGC	1 cut(s)	2712
BstMAI	GTCTC	5 cut(s)	1139, 1185, 2590, 3271, 3777
BstMCI	CGRYCG	1 cut(s)	3659
BstNI	CCWGG	9 cut(s)	64, 228, 449, 575, 1500, 1685, 2485, 3518, 3830
BstNSI	RCATGY	2 cut(s)	1397, 2094
BstPI	GGTNACC	1 cut(s)	321
BstSCI	CCNGG	9 cut(s)	62, 226, 447, 573, 1498, 1683, 2483, 3516, 3828
BstSFI	CTRYAG	7 cut(s)	418, 1176, 2714, 2850, 2892, 4179, 4482
BstSNI	TACGTA	1 cut(s)	3757
BstV2I	GAAGAC	4 cut(s)	134, 3122, 3425, 3654
BstX2I	RGATCY	5 cut(s)	29, 49, 166, 1165, 1267
BstXI	CCANNNNNNTGG	3 cut(s)	530, 1652, 2378
BstYI	RGATCY	5 cut(s)	29, 49, 166, 1165, 1267
BstZ17I	GTATAC	1 cut(s)	157
Bsu15I	ATCGAT	1 cut(s)	1461
Bsu36I	CCTNAGG	4 cut(s)	54, 2055, 2156, 3894
BsuI	GTATCC	1 cut(s)	3729
BsuRI	GGCC	8 cut(s)	1202, 1919, 2166, 2753, 2993, 3410, 3713, 4074
BsuTUI	ATCGAT	1 cut(s)	1461
BtsI	GCAGTG	2 cut(s)	1844, 2085
BtsIMutI	CAGTG	9 cut(s)	469, 1844, 2085, 3005, 3067, 3272, 3397, 3411, 3439
CaiI	CAGNNNCTG	2 cut(s)	695, 1175
CciI	TCATGA	1 cut(s)	3265
CfoI	GCGC	1 cut(s)	2712
Cfr10I	RCCGGY	1 cut(s)	1915
Cfr13I	GGNCC	4 cut(s)	2481, 2992, 3166, 3514
ClaI	ATCGAT	1 cut(s)	1461
CseI	GACGC	3 cut(s)	1202, 3167, 3689
Csp6I	GTAC	6 cut(s)	117, 495, 811, 895, 2924, 4019
CviQI	GTAC	6 cut(s)	117, 495, 811, 895, 2924, 4019
DinI	GGCGCC	1 cut(s)	2711
DraI	TTTAAA	3 cut(s)	246, 846, 2041
EaeI	YGGCCR	5 cut(s)	1200, 1917, 2164, 3408, 4072
Eam1104I	CTCTTC	8 cut(s)	93, 529, 1521, 2428, 2691, 2742, 3060, 4125
EarI	CTCTTC	8 cut(s)	93, 529, 1521, 2428, 2691, 2742, 3060, 4125
Eco105I	TACGTA	1 cut(s)	3757
Eco130I	CCWWGG	3 cut(s)	1035, 3646, 3905
Eco31I	GGTCTC	2 cut(s)	1139, 3271
Eco32I	GATATC	2 cut(s)	2000, 2129
Eco47I	GGWCC	3 cut(s)	2481, 3166, 3514
Eco57I	CTGAAG	4 cut(s)	647, 1800, 2409, 3891
Eco81I	CCTNAGG	4 cut(s)	54, 2055, 2156, 3894
Eco88I	CYCGRG	1 cut(s)	726
Eco91I	GGTNACC	1 cut(s)	321
EcoO109I	RGGNCCY	1 cut(s)	2992
EcoO65I	GGTNACC	1 cut(s)	321
EcoRII	CCWGG	9 cut(s)	62, 226, 447, 573, 1498, 1683, 2483, 3516, 3828
EcoRV	GATATC	2 cut(s)	2000, 2129
EcoT14I	CCWWGG	3 cut(s)	1035, 3646, 3905
EgeI	GGCGCC	1 cut(s)	2711
EheI	GGCGCC	1 cut(s)	2711
ErhI	CCWWGG	3 cut(s)	1035, 3646, 3905
Esp3I	CGTCTC	1 cut(s)	1185
FalI	AAGNNNNNCTT	4 cut(s)	1713, 1745, 3129, 3161
FauI	CCCGC	1 cut(s)	1952
FauNDI	CATATG	3 cut(s)	345, 525, 3534
FbaI	TGATCA	2 cut(s)	2944, 3529
FblI	GTMKAC	3 cut(s)	156, 192, 3000
FspBI	CTAG	9 cut(s)	150, 906, 2007, 2217, 2397, 3134, 3468, 4094, 4121
GlaI	GCGC	1 cut(s)	2711
GsuI	CTGGAG	5 cut(s)	596, 777, 2741, 3563, 4351
HaeII	RGCGCY	1 cut(s)	2713
HaeIII	GGCC	8 cut(s)	1202, 1919, 2166, 2753, 2993, 3410, 3713, 4074
HapII	CCGG	5 cut(s)	316, 480, 1916, 2527, 3722
HgaI	GACGC	3 cut(s)	1202, 3167, 3689
HhaI	GCGC	1 cut(s)	2712
Hin1I	GRCGYC	1 cut(s)	2710
Hin6I	GCGC	1 cut(s)	2710
HinP1I	GCGC	1 cut(s)	2710
HincII	GTYRAC	2 cut(s)	997, 2116
HindII	GTYRAC	2 cut(s)	997, 2116
HindIII	AAGCTT	4 cut(s)	1159, 1571, 2099, 2963
HpaI	GTTAAC	1 cut(s)	997
HpaII	CCGG	5 cut(s)	316, 480, 1916, 2527, 3722
HphI	GGTGA	5 cut(s)	379, 389, 1667, 3858, 4385
Hpy166II	GTNNAC	8 cut(s)	157, 193, 307, 389, 997, 1240, 2116, 3001
Hpy8I	GTNNAC	8 cut(s)	157, 193, 307, 389, 997, 1240, 2116, 3001
Hpy99I	CGWCG	1 cut(s)	2331
HpyAV	CCTTC	9 cut(s)	2340, 2830, 3389, 3630, 3790, 3866, 4046, 4072, 4424
HpyCH4III	ACNGT	7 cut(s)	116, 133, 139, 619, 2509, 3288, 3890
HpyCH4IV	ACGT	4 cut(s)	1123, 1600, 2842, 3756
HpySE526I	ACGT	4 cut(s)	1123, 1600, 2842, 3756
Hsp92I	GRCGYC	1 cut(s)	2710
HspAI	GCGC	1 cut(s)	2710
KasI	GGCGCC	1 cut(s)	2709
Kpn2I	TCCGGA	1 cut(s)	315
Ksp22I	TGATCA	2 cut(s)	2944, 3529
KspAI	GTTAAC	1 cut(s)	997
LguI	GCTCTTC	5 cut(s)	529, 1521, 2428, 2691, 3060
MaeI	CTAG	9 cut(s)	150, 906, 2007, 2217, 2397, 3134, 3468, 4094, 4121
MaeII	ACGT	4 cut(s)	1123, 1600, 2842, 3756
MbiI	CCGCTC	1 cut(s)	3070
MfeI	CAATTG	1 cut(s)	4425
MflI	RGATCY	5 cut(s)	29, 49, 166, 1165, 1267
MhlI	GDGCHC	2 cut(s)	2423, 2659
MlsI	TGGCCA	3 cut(s)	1202, 2166, 4074
MluNI	TGGCCA	3 cut(s)	1202, 2166, 4074
Mly113I	GGCGCC	1 cut(s)	2710
MlyI	GAGTC	7 cut(s)	96, 394, 587, 2527, 2996, 3317, 4358
MmeI	TCCRAC	3 cut(s)	7, 2027, 2573
Mox20I	TGGCCA	3 cut(s)	1202, 2166, 4074
MroI	TCCGGA	1 cut(s)	315
MroXI	GAANNNNTTC	1 cut(s)	311
MscI	TGGCCA	3 cut(s)	1202, 2166, 4074
MslI	CAYNNNNRTG	3 cut(s)	333, 528, 3345
Msp20I	TGGCCA	3 cut(s)	1202, 2166, 4074
MspA1I	CMGCKG	4 cut(s)	2429, 2719, 3583, 4371
MspI	CCGG	5 cut(s)	316, 480, 1916, 2527, 3722
MspR9I	CCNGG	9 cut(s)	64, 228, 449, 575, 1500, 1685, 2485, 3518, 3830
MunI	CAATTG	1 cut(s)	4425
MvaI	CCWGG	9 cut(s)	64, 228, 449, 575, 1500, 1685, 2485, 3518, 3830
NarI	GGCGCC	1 cut(s)	2710
NdeI	CATATG	3 cut(s)	345, 525, 3534
NheI	GCTAGC	2 cut(s)	2216, 4093
NmuCI	GTSAC	5 cut(s)	139, 367, 1132, 2083, 3370
NspI	RCATGY	2 cut(s)	1397, 2094
PaeI	GCATGC	1 cut(s)	2094
PagI	TCATGA	1 cut(s)	3265
PciSI	GCTCTTC	5 cut(s)	529, 1521, 2428, 2691, 3060
PdmI	GAANNNNTTC	1 cut(s)	311
PfoI	TCCNGGA	3 cut(s)	573, 1683, 2483
Ple19I	CGATCG	1 cut(s)	3659
PleI	GAGTC	7 cut(s)	95, 393, 586, 2526, 2996, 3316, 4358
PluTI	GGCGCC	1 cut(s)	2713
PpsI	GAGTC	7 cut(s)	95, 393, 586, 2526, 2996, 3316, 4358
Ppu21I	YACGTR	1 cut(s)	3757
PsiI	TTATAA	1 cut(s)	2409
Psp1406I	AACGTT	1 cut(s)	1600
Psp6I	CCWGG	9 cut(s)	62, 226, 447, 573, 1498, 1683, 2483, 3516, 3828
PspEI	GGTNACC	1 cut(s)	321
PspGI	CCWGG	9 cut(s)	62, 226, 447, 573, 1498, 1683, 2483, 3516, 3828
PspPI	GGNCC	4 cut(s)	2481, 2992, 3166, 3514
PstI	CTGCAG	5 cut(s)	1180, 2718, 2854, 2896, 4486
PstNI	CAGNNNCTG	2 cut(s)	695, 1175
PsuI	RGATCY	5 cut(s)	29, 49, 166, 1165, 1267
PvuI	CGATCG	1 cut(s)	3659
PvuII	CAGCTG	2 cut(s)	2429, 2719
RsaI	GTAC	6 cut(s)	118, 496, 812, 896, 2925, 4020
RsaNI	GTAC	6 cut(s)	117, 495, 811, 895, 2924, 4019
RseI	CAYNNNNRTG	3 cut(s)	333, 528, 3345
SapI	GCTCTTC	5 cut(s)	529, 1521, 2428, 2691, 3060
Sau96I	GGNCC	4 cut(s)	2481, 2992, 3166, 3514
SchI	GAGTC	7 cut(s)	96, 394, 587, 2527, 2996, 3317, 4358
ScrFI	CCNGG	9 cut(s)	64, 228, 449, 575, 1500, 1685, 2485, 3518, 3830
SduI	GDGCHC	2 cut(s)	2423, 2659
SfcI	CTRYAG	7 cut(s)	418, 1176, 2714, 2850, 2892, 4179, 4482
SfoI	GGCGCC	1 cut(s)	2711
SinI	GGWCC	3 cut(s)	2481, 3166, 3514
SmiI	ATTTAAAT	1 cut(s)	846
SmiMI	CAYNNNNRTG	3 cut(s)	333, 528, 3345
SmlI	CTYRAG	3 cut(s)	2120, 2987, 3074
SmoI	CTYRAG	3 cut(s)	2120, 2987, 3074
SnaBI	TACGTA	1 cut(s)	3757
SpeI	ACTAGT	1 cut(s)	3467
SphI	GCATGC	1 cut(s)	2094
SsiI	CCGC	9 cut(s)	553, 1959, 2623, 2882, 3070, 3411, 3458, 3583, 4371
SspDI	GGCGCC	1 cut(s)	2709
SspI	AATATT	1 cut(s)	957
SspMI	CTAG	9 cut(s)	150, 906, 2007, 2217, 2397, 3134, 3468, 4094, 4121
StyD4I	CCNGG	9 cut(s)	62, 226, 447, 573, 1498, 1683, 2483, 3516, 3828
StyI	CCWWGG	3 cut(s)	1035, 3646, 3905
SwaI	ATTTAAAT	1 cut(s)	846
TaaI	ACNGT	7 cut(s)	116, 133, 139, 619, 2509, 3288, 3890
TaiI	ACGT	4 cut(s)	1126, 1603, 2845, 3759
TaqI	TCGA	9 cut(s)	45, 253, 594, 1314, 1338, 1461, 2191, 2329, 3098
TaqII	GACCGA	1 cut(s)	3495
TatI	WGTACW	5 cut(s)	116, 494, 810, 2923, 4018
TauI	GCSGC	3 cut(s)	2885, 3073, 3413
TscAI	CASTG	9 cut(s)	469, 1851, 2092, 3012, 3067, 3279, 3397, 3411, 3446
TseFI	GTSAC	5 cut(s)	139, 367, 1132, 2083, 3370
Tsp45I	GTSAC	5 cut(s)	139, 367, 1132, 2083, 3370
TspGWI	ACGGA	1 cut(s)	3101
TspRI	CASTG	9 cut(s)	469, 1851, 2092, 3012, 3067, 3279, 3397, 3411, 3446
VpaK11BI	GGWCC	3 cut(s)	2481, 3166, 3514
XapI	RAATTY	8 cut(s)	173, 241, 1282, 2024, 2261, 2807, 3171, 3296
XceI	RCATGY	2 cut(s)	1397, 2094
XcmI	CCANNNNNNNNNTGG	2 cut(s)	16, 2677
XmiI	GTMKAC	3 cut(s)	156, 192, 3000
XmnI	GAANNNNTTC	1 cut(s)	311
XspI	CTAG	9 cut(s)	150, 906, 2007, 2217, 2397, 3134, 3468, 4094, 4121

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.