MD16G1281800.v1.1

starch synthase 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011846
Physical Location & Seq
Forward (+)
38419147 .. 38427149
8003 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1281800.v1.1.491

Sequence Viewer

Length: 2001 bp
ATGACGGCGCTGATGAGCAGCTCGATCCTCCGCCCGTGGCAGTGGCAACCACCGCATCAGAAACCTCCAACTCCACTAGTTTGCTGCTCCGCCGTCAATGCCTCCTCCTCCTCCTCCTCAGAAATGAAGGTTAAGGGCGATGGAAGTGCGGAGTCCGTCTCTCGGGCCGAAGGCATTTGGAAACTCTTCAGAGAAGCTCAGAAGAATATTCTGCAGTTAAACCAGCAACGCATTAAGGCGGTAGAAGAACTCAACAAAATCAACAGAGAGAAAGAGCTGTTGGTGGATAGGATTGAGCAACTGGAGCTGGAAAAGCAGGCTGCCATTGCAAGACCTCAAGATAGGGTAAGCTGTTGGGAGCTACTCTTTCGGATAGATTCAATGGTCCTCAATGGAATGGTTACTACCGTGGAGGCATCCGATTTGAGAAGGCTGGTTATGGATAACAAATTTAGCTTACCTGAAGTATTTAATGACACCCTGCAGAAAGGAGACACTGAGATTCTAGCTGAACTTCGTCACTTCTCCGAGAGAATCAAAAGGAATGGCTTTCACATTGTCCATGTCTGCACGGAAATGGCACCCCTGGTCTCTTTCGGATCCTTGGCTTCATATGTGACAGGCTTGTCTTGTGCACTACAAAGGAAGGGGAATTTGGTGGAGGTGATACTTCCAAAGTTTTCAAGCTTGGACTTAAACGAAGTTCGAGGCCTAAAAGAAGTTGAGGCAGAATGTTATTCGTATTACAATGGTCAGTTGCATGGGAACAAAATCTGGACCGGAGTTGTCTATGGCATTGGAGTTACATTAATTCAACCATTATACTACTCATCATTTTTCAATCGTGAGAGGCTATATGGCTACTCAGATGACTTTGAAAGGTTTACCTATTTCTCCCGTGCTTCATTGGATTATATTGTAAAATCTGGAAAGCGTCCTGATGTGATACATATCCACAACTGGGAGACTGCTATTATTGGGCCACTTTTCTGGGATATTACTGTTAAACAGGGACTTGAAGGTACAAGAATTCTATTGACATGCCATGACCTCAACTCACAGTGTCTTGAGCATCCAGAGAAACTAGAGTTATGCGGACTCGATCCTGCTAGACTTCACCGTCCTGATCGTTTGCAAGATAATACCAAGGCACATCTCGTTAATATCTTGAAGGGTGGAGTTGTTTACTCCAATAAAGTTGTCATTATGTCATCCATACTTTCAAAAGGCAGGGTTATTCAGCGTCTGAGTCATGGGTTGGATCCTACCTTGAACATTCACAAAAACAAAGTGATTATTGCTCCTTGTGGATTTGACAACTCCACCTGGGATCCGTCCACGGACAACTTTCTTCCTGTACAGTATAATGCGAAGGATATGAAAGGGAAAACAGTCTGCAAAGCTGCATTGCAGCAGCACCTTGGGTTATCCGAGCATGCTTCTACTTTTCTTGTTGGATGCATCTTCTCTAAAGTATCAGATGTTGATCTGGAGAAGCTGAGGGAAGTATTTTCGAAAACTAGCAGGATTGATGTACAGTTCATCGTCATGGGGATTGGCAAAATATCAAGCGTAAACAAGTTGGGATCATTGCATGAACCCTTGAAGGATGCAAATGTACAATTCATAGATGGGAATGATGAAGCGTTATCACATTTGGTTTTCGCAGGATCTGATATTATCTTATGCCAATCTTTTCATGACCCTGACCTTCAAGTTCCACTAAAAGCTTTAATATACGGAGCTGCTCCAATTACAGTAAACTCCAACGACGAGGGGTTCGGGAACTTTGTCGAACATAACTACGAGACCACTAATTTCTCACGTTTCATCAGCTCTGCGTTTGGAAATATGACTCTAAGCCAAGCCCTGGATGAAATTAAAAACAACCCGTCGAAGTGGAAGAGAAAGATAACGGATGCCATGGAAATGGACTACTCGTGGGATGCTGAGTGCTGCGACATCCATACTTCTGCGTATACAGCCTTGAAAAATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5

Protein Analysis

667

Amino Acids

74.96

Weight (kDa)

6.39

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 185 - 408 6e-36 Starch synthase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 625, 1119
AccB1I GGYRCC 1 cut(s) 580
AccB7I CCANNNNNTGG 1 cut(s) 1870
AccI GTMKAC 1 cut(s) 1979
AciI CCGC 6 cut(s) 31, 53, 90, 149, 239, 1095
AcsI RAATTY 4 cut(s) 449, 652, 1029, 1993
AcuI CTGAAG 2 cut(s) 172, 483
AfaI GTAC 4 cut(s) 1024, 1359, 1536, 1620
AfiI CCNNNNNNNGG 3 cut(s) 162, 961, 1870
AhlI ACTAGT 1 cut(s) 76
AjnI CCWGG 3 cut(s) 585, 1325, 1869
AjuI GAANNNNNNNTTGG 4 cut(s) 263, 295, 638, 670
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 5 cut(s) 163, 486, 595, 959, 1802
Alw44I GTGCAC 1 cut(s) 633
AlwNI CAGNNNCTG 2 cut(s) 1246, 1673
Ama87I CYCGRG 1 cut(s) 162
AoxI GGCC 3 cut(s) 165, 709, 980
ApaLI GTGCAC 1 cut(s) 633
ApeKI GCWGC 8 cut(s) 18, 84, 320, 1403, 1411, 1414, 1745, 1956
ApoI RAATTY 4 cut(s) 449, 652, 1029, 1993
ArsI GACNNNNNNTTYG 2 cut(s) 1763, 1795
AseI ATTAAT 1 cut(s) 809
Asp700I GAANNNNTTC 1 cut(s) 185
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 4 cut(s) 165, 385, 777, 980
AsuHPI GGTGA 2 cut(s) 676, 1109
AsuII TTCGAA 1 cut(s) 1514
AvaI CYCGRG 1 cut(s) 162
AvaII GGWCC 2 cut(s) 385, 777
BaeGI GKGCMC 1 cut(s) 637
BamHI GGATCC 3 cut(s) 599, 1261, 1330
BanI GGYRCC 1 cut(s) 580
BauI CACGAG 1 cut(s) 1939
Bbv12I GWGCWC 1 cut(s) 637
BbvCI CCTCAGC 1 cut(s) 1499
BbvI GCAGC 8 cut(s) 30, 71, 307, 1390, 1423, 1426, 1732, 1943
BccI CCATC 2 cut(s) 134, 1625
BceAI ACGGC 2 cut(s) 21, 77
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BciT130I CCWGG 3 cut(s) 587, 1327, 1871
BcoDI GTCTC 5 cut(s) 163, 486, 595, 959, 1802
BcuI ACTAGT 1 cut(s) 76
BfaI CTAG 5 cut(s) 77, 506, 1085, 1110, 1521
BfmI CTRYAG 2 cut(s) 212, 482
BfoI RGCGCY 1 cut(s) 11
BisI GCNGC 8 cut(s) 19, 85, 321, 1404, 1412, 1415, 1746, 1957
BlsI GCNGC 8 cut(s) 20, 86, 322, 1405, 1413, 1416, 1747, 1958
Bme1390I CCNGG 3 cut(s) 587, 1327, 1871
Bme18I GGWCC 2 cut(s) 385, 777
BmeT110I CYCGRG 1 cut(s) 162
BmgT120I GGNCC 4 cut(s) 165, 385, 777, 980
BmiI GGNNCC 4 cut(s) 582, 601, 1263, 1332
BmrFI CCNGG 3 cut(s) 587, 1327, 1871
BmrI ACTGGG 1 cut(s) 970
BmsI GCATC 8 cut(s) 64, 425, 1081, 1448, 1470, 1600, 1909, 1936
BmuI ACTGGG 1 cut(s) 970
BplI GAGNNNNNCTC 2 cut(s) 143, 175
BpmI CTGGAG 2 cut(s) 323, 1511
Bpu10I CCTNAGC 1 cut(s) 1499
Bpu14I TTCGAA 1 cut(s) 1514
BpuEI CTTGAG 2 cut(s) 321, 1088
BsaBI GATNNNNATC 2 cut(s) 950, 1485
BsaI GGTCTC 2 cut(s) 595, 1802
BsaWI WCCGGW 1 cut(s) 779
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 961, 1870
Bse1I ACTGG 2 cut(s) 306, 965
Bse3DI GCAATG 3 cut(s) 324, 1406, 1589
Bse8I GATNNNNATC 2 cut(s) 950, 1485
BseBI CCWGG 3 cut(s) 587, 1327, 1871
BseGI GGATG 9 cut(s) 416, 1072, 1211, 1463, 1615, 1879, 1924, 1951, 1962
BseJI GATNNNNATC 2 cut(s) 950, 1485
BseLI CCNNNNNNNGG 3 cut(s) 162, 961, 1870
BseMI GCAATG 3 cut(s) 324, 1406, 1589
BseMII CTCAG 7 cut(s) 132, 212, 489, 879, 1238, 1490, 1941
BseNI ACTGG 2 cut(s) 306, 965
BseRI GAGGAG 5 cut(s) 94, 97, 100, 103, 106
BseSI GKGCMC 1 cut(s) 637
BseXI GCAGC 8 cut(s) 30, 71, 307, 1390, 1423, 1426, 1732, 1943
BsgI GTGCAG 1 cut(s) 553
BshFI GGCC 3 cut(s) 167, 711, 982
BshNI GGYRCC 1 cut(s) 580
BsiHKAI GWGCWC 1 cut(s) 637
BsiHKCI CYCGRG 1 cut(s) 162
BsiSI CCGG 1 cut(s) 780
BslFI GGGAC 1 cut(s) 1026
BslI CCNNNNNNNGG 3 cut(s) 162, 961, 1870
BsmAI GTCTC 5 cut(s) 163, 486, 595, 959, 1802
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 1026
BsnI GGCC 3 cut(s) 167, 711, 982
Bso31I GGTCTC 2 cut(s) 595, 1802
BsoBI CYCGRG 1 cut(s) 162
Bsp119I TTCGAA 1 cut(s) 1514
Bsp1286I GDGCHC 1 cut(s) 637
Bsp1407I TGTACA 3 cut(s) 1357, 1534, 1618
Bsp143I GATC 9 cut(s) 24, 599, 1102, 1126, 1261, 1330, 1486, 1586, 1670
Bsp19I CCATGG 1 cut(s) 1923
BspACI CCGC 6 cut(s) 31, 53, 90, 149, 239, 1095
BspANI GGCC 3 cut(s) 167, 711, 982
BspCNI CTCAG 7 cut(s) 131, 211, 490, 878, 1239, 1491, 1942
BspHI TCATGA 1 cut(s) 1699
BspLI GGNNCC 4 cut(s) 582, 601, 1263, 1332
BspMAI CTGCAG 2 cut(s) 216, 486
BspT104I TTCGAA 1 cut(s) 1514
BspT107I GGYRCC 1 cut(s) 580
BspTNI GGTCTC 2 cut(s) 595, 1802
BsrDI GCAATG 3 cut(s) 324, 1406, 1589
BsrGI TGTACA 3 cut(s) 1357, 1534, 1618
BsrI ACTGG 2 cut(s) 306, 965
BssMI GATC 9 cut(s) 24, 599, 1102, 1126, 1261, 1330, 1486, 1586, 1670
BssNAI GTATAC 1 cut(s) 1980
BssSI CACGAG 1 cut(s) 1939
BssT1I CCWWGG 4 cut(s) 603, 1146, 1420, 1923
Bst1107I GTATAC 1 cut(s) 1980
Bst2BI CACGAG 1 cut(s) 1939
Bst2UI CCWGG 3 cut(s) 587, 1327, 1871
Bst4CI ACNGT 8 cut(s) 409, 1003, 1062, 1121, 1362, 1393, 1539, 1759
Bst6I CTCTTC 2 cut(s) 191, 1898
BstAUI TGTACA 3 cut(s) 1357, 1534, 1618
BstBI TTCGAA 1 cut(s) 1514
BstC8I GCNNGC 2 cut(s) 318, 1437
BstDEI CTNAG 8 cut(s) 118, 198, 498, 865, 1247, 1499, 1859, 1950
BstDSI CCRYGG 4 cut(s) 35, 408, 1338, 1923
BstF5I GGATG 9 cut(s) 416, 1072, 1211, 1463, 1615, 1879, 1924, 1951, 1962
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 9 cut(s) 27, 602, 1105, 1129, 1264, 1333, 1489, 1589, 1673
BstMAI GTCTC 5 cut(s) 163, 486, 595, 959, 1802
BstMBI GATC 9 cut(s) 24, 599, 1102, 1126, 1261, 1330, 1486, 1586, 1670
BstMWI GCNNNNNNNGC 6 cut(s) 52, 98, 304, 313, 326, 1982
BstNI CCWGG 3 cut(s) 587, 1327, 1871
BstNSI RCATGY 2 cut(s) 1044, 1439
BstSCI CCNGG 3 cut(s) 585, 1325, 1869
BstSFI CTRYAG 2 cut(s) 212, 482
BstSLI GKGCMC 1 cut(s) 637
BstV1I GCAGC 8 cut(s) 30, 71, 307, 1390, 1423, 1426, 1732, 1943
BstX2I RGATCY 4 cut(s) 599, 1261, 1330, 1670
BstXI CCANNNNNNTGG 2 cut(s) 990, 1930
BstYI RGATCY 4 cut(s) 599, 1261, 1330, 1670
BstZ17I GTATAC 1 cut(s) 1980
BsuRI GGCC 3 cut(s) 167, 711, 982
BtgI CCRYGG 4 cut(s) 35, 408, 1338, 1923
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 9 cut(s) 416, 1072, 1211, 1463, 1615, 1879, 1924, 1951, 1962
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 3 cut(s) 47, 495, 1067
Cac8I GCNNGC 2 cut(s) 318, 1437
CaiI CAGNNNCTG 2 cut(s) 1246, 1673
CciI TCATGA 1 cut(s) 1699
CfoI GCGC 1 cut(s) 10
Cfr13I GGNCC 4 cut(s) 165, 385, 777, 980
CseI GACGC 2 cut(s) 923, 1232
Csp6I GTAC 4 cut(s) 1023, 1358, 1535, 1619
CviQI GTAC 4 cut(s) 1023, 1358, 1535, 1619
DdeI CTNAG 8 cut(s) 118, 198, 498, 865, 1247, 1499, 1859, 1950
DpnI GATC 9 cut(s) 26, 601, 1104, 1128, 1263, 1332, 1488, 1588, 1672
DpnII GATC 9 cut(s) 24, 599, 1102, 1126, 1261, 1330, 1486, 1586, 1670
DrdI GACNNNNNNGTC 2 cut(s) 625, 1119
DseDI GACNNNNNNGTC 2 cut(s) 625, 1119
Eam1104I CTCTTC 2 cut(s) 191, 1898
EarI CTCTTC 2 cut(s) 191, 1898
EciI GGCGGA 2 cut(s) 20, 79
Eco130I CCWWGG 4 cut(s) 603, 1146, 1420, 1923
Eco147I AGGCCT 1 cut(s) 711
Eco31I GGTCTC 2 cut(s) 595, 1802
Eco47I GGWCC 2 cut(s) 385, 777
Eco57I CTGAAG 2 cut(s) 172, 483
Eco88I CYCGRG 1 cut(s) 162
EcoRI GAATTC 1 cut(s) 1029
EcoRII CCWGG 3 cut(s) 585, 1325, 1869
EcoT14I CCWWGG 4 cut(s) 603, 1146, 1420, 1923
EcoT22I ATGCAT 1 cut(s) 1463
ErhI CCWWGG 4 cut(s) 603, 1146, 1420, 1923
Esp3I CGTCTC 1 cut(s) 163
FaqI GGGAC 1 cut(s) 1026
FauNDI CATATG 1 cut(s) 613
FblI GTMKAC 1 cut(s) 1979
Fnu4HI GCNGC 8 cut(s) 19, 85, 321, 1404, 1412, 1415, 1746, 1957
FokI GGATG 9 cut(s) 403, 1059, 1198, 1470, 1622, 1886, 1931, 1949, 1958
Fsp4HI GCNGC 8 cut(s) 19, 85, 321, 1404, 1412, 1415, 1746, 1957
FspBI CTAG 5 cut(s) 77, 506, 1085, 1110, 1521
GlaI GCGC 1 cut(s) 9
GluI GCNGC 8 cut(s) 19, 85, 321, 1404, 1412, 1415, 1746, 1957
GsuI CTGGAG 2 cut(s) 323, 1511
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 3 cut(s) 167, 711, 982
HapII CCGG 1 cut(s) 780
HgaI GACGC 2 cut(s) 923, 1232
HhaI GCGC 1 cut(s) 10
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HindIII AAGCTT 2 cut(s) 685, 1728
HinfI GANTC 7 cut(s) 152, 377, 502, 534, 1098, 1249, 1855
HpaII CCGG 1 cut(s) 780
HphI GGTGA 2 cut(s) 676, 1109
Hpy166II GTNNAC 7 cut(s) 635, 885, 1186, 1338, 1576, 1762, 1980
Hpy8I GTNNAC 7 cut(s) 635, 885, 1186, 1338, 1576, 1762, 1980
Hpy99I CGWCG 2 cut(s) 1775, 1897
HpyAV CCTTC 9 cut(s) 121, 164, 423, 640, 1013, 1165, 1366, 1600, 1721
HpyCH4III ACNGT 8 cut(s) 409, 1003, 1062, 1121, 1362, 1393, 1539, 1759
HpyCH4IV ACGT 1 cut(s) 1825
HpyF10VI GCNNNNNNNGC 6 cut(s) 52, 98, 304, 313, 326, 1982
HpyF3I CTNAG 8 cut(s) 118, 198, 498, 865, 1247, 1499, 1859, 1950
HpySE526I ACGT 1 cut(s) 1825
HspAI GCGC 1 cut(s) 8
Kzo9I GATC 9 cut(s) 24, 599, 1102, 1126, 1261, 1330, 1486, 1586, 1670
LmnI GCTCC 6 cut(s) 92, 304, 358, 1306, 1742, 1753
Lsp1109I GCAGC 8 cut(s) 30, 71, 307, 1390, 1423, 1426, 1732, 1943
LweI GCATC 8 cut(s) 64, 425, 1081, 1448, 1470, 1600, 1909, 1936
MaeI CTAG 5 cut(s) 77, 506, 1085, 1110, 1521
MaeII ACGT 1 cut(s) 1825
MaeIII GTNAC 4 cut(s) 400, 518, 616, 802
MalI GATC 9 cut(s) 26, 601, 1104, 1128, 1263, 1332, 1488, 1588, 1672
MboI GATC 9 cut(s) 24, 599, 1102, 1126, 1261, 1330, 1486, 1586, 1670
MboII GAAGA 6 cut(s) 178, 214, 257, 1343, 1456, 1915
MflI RGATCY 4 cut(s) 599, 1261, 1330, 1670
MhlI GDGCHC 1 cut(s) 637
MluCI AATT 9 cut(s) 449, 652, 810, 1029, 1622, 1752, 1816, 1878, 1993
MlyI GAGTC 4 cut(s) 161, 1092, 1258, 1849
MmeI TCCRAC 4 cut(s) 92, 1239, 1435, 1791
Mph1103I ATGCAT 1 cut(s) 1463
MroXI GAANNNNTTC 1 cut(s) 185
MslI CAYNNNNRTG 3 cut(s) 575, 1547, 1928
MspI CCGG 1 cut(s) 780
MspR9I CCNGG 3 cut(s) 587, 1327, 1871
MvaI CCWGG 3 cut(s) 587, 1327, 1871
MwoI GCNNNNNNNGC 6 cut(s) 52, 98, 304, 313, 326, 1982
NcoI CCATGG 1 cut(s) 1923
NdeI CATATG 1 cut(s) 613
NdeII GATC 9 cut(s) 24, 599, 1102, 1126, 1261, 1330, 1486, 1586, 1670
NlaIV GGNNCC 4 cut(s) 582, 601, 1263, 1332
NmuCI GTSAC 2 cut(s) 518, 616
NsiI ATGCAT 1 cut(s) 1463
NspI RCATGY 2 cut(s) 1044, 1439
NspV TTCGAA 1 cut(s) 1514
PaeI GCATGC 1 cut(s) 1439
PagI TCATGA 1 cut(s) 1699
PceI AGGCCT 1 cut(s) 711
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 3 cut(s) 377, 502, 534
PflMI CCANNNNNTGG 1 cut(s) 1870
PkrI GCNGC 8 cut(s) 20, 86, 322, 1405, 1413, 1416, 1747, 1958
PleI GAGTC 4 cut(s) 160, 1092, 1257, 1849
PpsI GAGTC 4 cut(s) 160, 1092, 1257, 1849
PshBI ATTAAT 1 cut(s) 809
Psp6I CCWGG 3 cut(s) 585, 1325, 1869
PspGI CCWGG 3 cut(s) 585, 1325, 1869
PspN4I GGNNCC 4 cut(s) 582, 601, 1263, 1332
PspPI GGNCC 4 cut(s) 165, 385, 777, 980
PstI CTGCAG 2 cut(s) 216, 486
PstNI CAGNNNCTG 2 cut(s) 1246, 1673
PsuI RGATCY 4 cut(s) 599, 1261, 1330, 1670
RsaI GTAC 4 cut(s) 1024, 1359, 1536, 1620
RsaNI GTAC 4 cut(s) 1023, 1358, 1535, 1619
RseI CAYNNNNRTG 3 cut(s) 575, 1547, 1928
SatI GCNGC 8 cut(s) 19, 85, 321, 1404, 1412, 1415, 1746, 1957
Sau3AI GATC 9 cut(s) 24, 599, 1102, 1126, 1261, 1330, 1486, 1586, 1670
Sau96I GGNCC 4 cut(s) 165, 385, 777, 980
SchI GAGTC 4 cut(s) 161, 1092, 1258, 1849
ScrFI CCNGG 3 cut(s) 587, 1327, 1871
SduI GDGCHC 1 cut(s) 637
SfaNI GCATC 8 cut(s) 64, 425, 1081, 1448, 1470, 1600, 1909, 1936
SfcI CTRYAG 2 cut(s) 212, 482
SfuI TTCGAA 1 cut(s) 1514
SinI GGWCC 2 cut(s) 385, 777
SmiMI CAYNNNNRTG 3 cut(s) 575, 1547, 1928
SmlI CTYRAG 2 cut(s) 336, 1067
SmoI CTYRAG 2 cut(s) 336, 1067
SpeI ACTAGT 1 cut(s) 76
SphI GCATGC 1 cut(s) 1439
Sse9I AATT 9 cut(s) 449, 652, 810, 1029, 1622, 1752, 1816, 1878, 1993
SseBI AGGCCT 1 cut(s) 711
SsiI CCGC 6 cut(s) 31, 53, 90, 149, 239, 1095
SspI AATATT 1 cut(s) 208
SspMI CTAG 5 cut(s) 77, 506, 1085, 1110, 1521
StuI AGGCCT 1 cut(s) 711
StyD4I CCNGG 3 cut(s) 585, 1325, 1869
StyI CCWWGG 4 cut(s) 603, 1146, 1420, 1923
TaaI ACNGT 8 cut(s) 409, 1003, 1062, 1121, 1362, 1393, 1539, 1759
TaiI ACGT 1 cut(s) 1828
TaqI TCGA 6 cut(s) 23, 706, 1101, 1514, 1794, 1895
TasI AATT 9 cut(s) 449, 652, 810, 1029, 1622, 1752, 1816, 1878, 1993
TatI WGTACW 3 cut(s) 1357, 1534, 1618
TfiI GAWTC 3 cut(s) 377, 502, 534
TscAI CASTG 3 cut(s) 47, 502, 1067
TseFI GTSAC 2 cut(s) 518, 616
TseI GCWGC 8 cut(s) 18, 84, 320, 1403, 1411, 1414, 1745, 1956
Tsp45I GTSAC 2 cut(s) 518, 616
TspGWI ACGGA 6 cut(s) 145, 587, 1323, 1355, 1755, 1931
TspRI CASTG 3 cut(s) 47, 502, 1067
Van91I CCANNNNNTGG 1 cut(s) 1870
VneI GTGCAC 1 cut(s) 633
VpaK11BI GGWCC 2 cut(s) 385, 777
VspI ATTAAT 1 cut(s) 809
XapI RAATTY 4 cut(s) 449, 652, 1029, 1993
XceI RCATGY 2 cut(s) 1044, 1439
XmiI GTMKAC 1 cut(s) 1979
XmnI GAANNNNTTC 1 cut(s) 185
XspI CTAG 5 cut(s) 77, 506, 1085, 1110, 1521
Zsp2I ATGCAT 1 cut(s) 1463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.