Prupe.6G211300_v2.0.a1

starch synthase 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011846
Physical Location & Seq
Forward (+)
21961037 .. 21969612
8576 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G211300.3

Sequence Viewer

Length: 2019 bp
ATGAAGGCGCTGTTGAGCAGTGTGATCATCCCTTGGCAACAACCCCACCCAAAACCTCGAATTCCAGTTGTTTGCTGCTCCACCATTAATGGGTCCTCATCCTCTTCCTCAGAAGCGAACTTACAAAGCTCCCAGCCTCAGGAGTGCAAGTCATCCAATGCTGAGGAACCGAAACAGTCCGAAATTTGGCGACTCTTCAGAGAAGCTCAGAAGAATATTCTACACTTGAACCAACATCGTCTTAAGGCGGTAGAAGATCTTAACAAAATCAACATAGAGAAACAACTGTTGGTTGATAGGATAGAGCAATTGGAGCTGGAAAAGCAGACTGCTGTTGCAAAACCTCAAGATAGGCTATCAATGTTTTGGGAGCTACTTCTTAGGATAGACTCAATGGTCCTCATGGGAATGGTTACCATTGGGGAGGCATCTAATTTGAGAAGGCTGGTCATGGATCACAAAGTTAGCGTAGCTGAAGTATTTAACGACACCCTGCAGAAGAGTGATGCTGAGATTTTAGCAGAACTCCGGCACTTCTTGGAGAGAAACCAAAGATTCAGGAATGGATTTCACATTGTCCATGTATGCACGGAAATGGCACCACTGGTCTCCGTTGGATCTTTGGCTTCATACGTGACAGGTTTGTCTTGTGCACTACAAAGGAAGGGGCATTTGGTGGAGGTGATACTTCCAAAGTATTCAAACTTGGATTTAGATGAAGTTCAAGGGCTACAGGAAATTGAGGCAGAGTGTTATTCATATTTTAATGGTCAGTTGCATGGCAACAGAATTTGGACTGGTGTTGTCTATGGTATTGGAGTTACTTTAATTCAACCACTATACTACTCATCCTTTTTCAACCGTGAGAGGGTATATGGCTATTCAGATGACTTTGAAAGGTTTACCTATTTCTCCCGTGCTTCATTGGATTATATTGTAAAATCTGGAAAGCAACCTGATGTGATACATATCCACAACTGGGAGACTGCCATTATTGGGCCACTTTTCTGGGATATTATTGTTAAACAGGGACTTGAAGGTACAAGAGTTCTATTGACATGCCACGACCTCAATTCACAGTGTCTTGAGCATCCGGAGAAACTAGCGTTATGTGGACTTGATCCTGCTAGACTTCACCGTCCTGATCGTTTGCAAGATACTACCAAGGCACATCTGGTTAATGTTTTGAAGGGTGGAGTTGTTTACTCCAATAAAGTTGTCATTATGTCATCCATACATTCAAAAGGCAGGGTTATTCATAGTCTGAGTCATGGGTTGGATCATACCTTAAACATTCACAAAAACAAAGTGGTTATTGCTCCTTGTGGATTTGACAACTGCACTTGGGATCCATCTACAGACAACTTTCTTCCCCAACAGTATAGTGTGAAGGATATGAAAGGGAAAGCAGTCTGCAAAGCTGCATTGCAGCAGCACCTTGGGTTATCTGAGCATTCTTCTACTATTCTTGTCGGATGCATCTTCTCTAAAGTATCAGATGTTGATCTGGAGAACCTGAGGGCAGTATTTCGGAAAGCTAGGAGGATTGATGTGCAGTTCATCATTAAGGGGATTAGCAAAATATCAAGTGTAAACAAGCTGGGATTGGTGCATGAACCACTGAAGGATAAAAATGTACGATTCATAGATGGACATGATGAAAAGCAATCACATTTGATATTCGCCGGATCTGATATTATCTTATGTCAATCTTTTCATGATCCTGTCCTCCAAGTTCCACTAAAGGCTTTAAAGTATGGGGCCGCTCCAATTGCAGTAAACTCCAATGATGACGGGTTCAGGAACTTTGTGGAACATGACTATGAGACCACTAACTTCTCACGTTTCATTAGCTCTACTTTTGGAAATATGTCTATAACCCAAGCCCTGGATGAAATTAGAAACAACCCGTCGAAGTGGAAGAGAAAGATAATGGATGCCATGGAAATGGACTTCTCATGGGATGCTGAGTGCTGTGACATCCATGCTTCTGCTTATACAGCCATAAAGAATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5

Protein Analysis

673

Amino Acids

76.04

Weight (kDa)

6.98

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 395, 643, 1137
AccB1I GGYRCC 1 cut(s) 598
AccB7I CCANNNNNTGG 1 cut(s) 1888
AccBSI CCGCTC 1 cut(s) 1766
AccIII TCCGGA 1 cut(s) 1093
AciI CCGC 2 cut(s) 248, 1764
AclWI GGATC 8 cut(s) 462, 625, 1115, 1287, 1343, 1356, 1696, 1715
AcsI RAATTY 4 cut(s) 60, 183, 789, 2011
AcuI CTGAAG 3 cut(s) 181, 495, 1643
AfaI GTAC 2 cut(s) 1042, 1638
AfiI CCNNNNNNNGG 7 cut(s) 90, 139, 186, 868, 979, 996, 1888
AflII CTTAAG 1 cut(s) 242
AgsI TTSAA 9 cut(s) 229, 702, 725, 833, 859, 896, 1037, 1189, 1242
AjnI CCWGG 1 cut(s) 1887
AjuI GAANNNNNNNTTGG 4 cut(s) 272, 304, 656, 688
AluBI AGCT 9 cut(s) 129, 206, 316, 373, 473, 1421, 1538, 1600, 1854
AluI AGCT 9 cut(s) 129, 206, 316, 373, 473, 1421, 1538, 1600, 1854
Alw21I GWGCWC 1 cut(s) 655
Alw26I GTCTC 3 cut(s) 613, 977, 1820
Alw44I GTGCAC 1 cut(s) 651
AlwI GGATC 8 cut(s) 462, 625, 1115, 1287, 1343, 1356, 1696, 1715
Aor13HI TCCGGA 1 cut(s) 1093
AoxI GGCC 2 cut(s) 998, 1761
ApaLI GTGCAC 1 cut(s) 651
ApeKI GCWGC 4 cut(s) 75, 1421, 1429, 1432
ApoI RAATTY 4 cut(s) 60, 183, 789, 2011
AseI ATTAAT 1 cut(s) 87
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 4 cut(s) 93, 397, 998, 1761
AsuHPI GGTGA 2 cut(s) 694, 1127
AvaII GGWCC 2 cut(s) 93, 397
AxyI CCTNAGG 2 cut(s) 138, 1517
BaeGI GKGCMC 1 cut(s) 655
BaeI ACNNNNGTAYC 2 cut(s) 1032, 1065
BamHI GGATCC 1 cut(s) 1348
BanI GGYRCC 1 cut(s) 598
BarI GAAGNNNNNNTAC 2 cut(s) 105, 137
Bbv12I GWGCWC 1 cut(s) 655
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 4 cut(s) 62, 1408, 1441, 1444
BccI CCATC 2 cut(s) 1360, 1643
BciT130I CCWGG 1 cut(s) 1889
BclI TGATCA 1 cut(s) 24
BcoDI GTCTC 3 cut(s) 613, 977, 1820
BfaI CTAG 3 cut(s) 1103, 1128, 1539
BfmI CTRYAG 3 cut(s) 494, 731, 1356
BfoI RGCGCY 1 cut(s) 11
BfrI CTTAAG 1 cut(s) 242
BglII AGATCT 1 cut(s) 256
BisI GCNGC 5 cut(s) 76, 1422, 1430, 1433, 1764
BlsI GCNGC 5 cut(s) 77, 1423, 1431, 1434, 1765
Bme1390I CCNGG 1 cut(s) 1889
Bme18I GGWCC 2 cut(s) 93, 397
BmgT120I GGNCC 4 cut(s) 93, 397, 998, 1761
BmiI GGNNCC 5 cut(s) 94, 168, 600, 1350, 1762
BmrFI CCNGG 1 cut(s) 1889
BmrI ACTGGG 1 cut(s) 988
BmsI GCATC 7 cut(s) 437, 496, 1099, 1466, 1488, 1927, 1954
BmuI ACTGGG 1 cut(s) 988
BpmI CTGGAG 1 cut(s) 1529
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 2 cut(s) 330, 1106
BsaAI YACGTR 1 cut(s) 634
BsaBI GATNNNNATC 2 cut(s) 968, 1503
BsaI GGTCTC 2 cut(s) 613, 1820
BsaJI CCNNGG 5 cut(s) 32, 1164, 1438, 1887, 1941
BsaWI WCCGGW 1 cut(s) 1093
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 7 cut(s) 90, 139, 186, 868, 979, 996, 1888
Bse1I ACTGG 4 cut(s) 65, 609, 802, 983
Bse21I CCTNAGG 2 cut(s) 138, 1517
Bse3DI GCAATG 1 cut(s) 1424
Bse8I GATNNNNATC 2 cut(s) 968, 1503
BseAI TCCGGA 1 cut(s) 1093
BseBI CCWGG 1 cut(s) 1889
BseDI CCNNGG 5 cut(s) 32, 1164, 1438, 1887, 1941
BseJI GATNNNNATC 2 cut(s) 968, 1503
BseLI CCNNNNNNNGG 7 cut(s) 90, 139, 186, 868, 979, 996, 1888
BseMI GCAATG 1 cut(s) 1424
BseMII CTCAG 9 cut(s) 123, 152, 153, 221, 501, 1256, 1440, 1508, 1959
BseNI ACTGG 4 cut(s) 65, 609, 802, 983
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 4 cut(s) 62, 1408, 1441, 1444
BseYI CCCAGC 2 cut(s) 132, 1600
BsgI GTGCAG 2 cut(s) 1324, 1574
BshFI GGCC 2 cut(s) 1000, 1763
BshNI GGYRCC 1 cut(s) 598
BsiHKAI GWGCWC 1 cut(s) 655
BsiSI CCGG 3 cut(s) 529, 1094, 1686
BslFI GGGAC 1 cut(s) 1044
BslI CCNNNNNNNGG 7 cut(s) 90, 139, 186, 868, 979, 996, 1888
BsmAI GTCTC 3 cut(s) 613, 977, 1820
BsmFI GGGAC 1 cut(s) 1044
BsmI GAATGC 1 cut(s) 1453
BsnI GGCC 2 cut(s) 1000, 1763
Bso31I GGTCTC 2 cut(s) 613, 1820
Bsp1286I GDGCHC 1 cut(s) 655
Bsp13I TCCGGA 1 cut(s) 1093
Bsp19I CCATGG 1 cut(s) 1941
BspACI CCGC 2 cut(s) 248, 1764
BspANI GGCC 2 cut(s) 1000, 1763
BspCNI CTCAG 9 cut(s) 122, 151, 154, 220, 502, 1257, 1441, 1509, 1960
BspEI TCCGGA 1 cut(s) 1093
BspHI TCATGA 1 cut(s) 1717
BspLI GGNNCC 5 cut(s) 94, 168, 600, 1350, 1762
BspMAI CTGCAG 1 cut(s) 498
BspPI GGATC 8 cut(s) 462, 625, 1115, 1287, 1343, 1356, 1696, 1715
BspT107I GGYRCC 1 cut(s) 598
BspTI CTTAAG 1 cut(s) 242
BspTNI GGTCTC 2 cut(s) 613, 1820
BsrBI CCGCTC 1 cut(s) 1766
BsrDI GCAATG 1 cut(s) 1424
BsrI ACTGG 4 cut(s) 65, 609, 802, 983
BssECI CCNNGG 5 cut(s) 32, 1164, 1438, 1887, 1941
BssT1I CCWWGG 4 cut(s) 32, 1164, 1438, 1941
Bst2UI CCWGG 1 cut(s) 1889
Bst4CI ACNGT 6 cut(s) 177, 288, 863, 1080, 1139, 1380
Bst6I CTCTTC 4 cut(s) 109, 200, 494, 1916
BstAFI CTTAAG 1 cut(s) 242
BstBAI YACGTR 1 cut(s) 634
BstDSI CCRYGG 1 cut(s) 1941
BstEII GGTNACC 1 cut(s) 412
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstMAI GTCTC 3 cut(s) 613, 977, 1820
BstMWI GCNNNNNNNGC 4 cut(s) 313, 322, 1772, 2000
BstNI CCWGG 1 cut(s) 1889
BstNSI RCATGY 1 cut(s) 1062
BstPI GGTNACC 1 cut(s) 412
BstSCI CCNGG 1 cut(s) 1887
BstSFI CTRYAG 3 cut(s) 494, 731, 1356
BstSLI GKGCMC 1 cut(s) 655
BstV1I GCAGC 4 cut(s) 62, 1408, 1441, 1444
BstX2I RGATCY 4 cut(s) 256, 617, 1348, 1688
BstXI CCANNNNNNTGG 2 cut(s) 1008, 1948
BstYI RGATCY 4 cut(s) 256, 617, 1348, 1688
Bsu36I CCTNAGG 2 cut(s) 138, 1517
BsuRI GGCC 2 cut(s) 1000, 1763
BtgI CCRYGG 1 cut(s) 1941
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 4 cut(s) 25, 602, 1085, 1619
CciI TCATGA 1 cut(s) 1717
CfoI GCGC 1 cut(s) 10
Cfr13I GGNCC 4 cut(s) 93, 397, 998, 1761
Csp6I GTAC 2 cut(s) 1041, 1637
CviQI GTAC 2 cut(s) 1041, 1637
DraI TTTAAA 1 cut(s) 1752
DrdI GACNNNNNNGTC 3 cut(s) 395, 643, 1137
DseDI GACNNNNNNGTC 3 cut(s) 395, 643, 1137
Eam1104I CTCTTC 4 cut(s) 109, 200, 494, 1916
EarI CTCTTC 4 cut(s) 109, 200, 494, 1916
Eco130I CCWWGG 4 cut(s) 32, 1164, 1438, 1941
Eco31I GGTCTC 2 cut(s) 613, 1820
Eco47I GGWCC 2 cut(s) 93, 397
Eco57I CTGAAG 3 cut(s) 181, 495, 1643
Eco81I CCTNAGG 2 cut(s) 138, 1517
Eco91I GGTNACC 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 93
EcoO65I GGTNACC 1 cut(s) 412
EcoRI GAATTC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 1887
EcoT14I CCWWGG 4 cut(s) 32, 1164, 1438, 1941
EcoT22I ATGCAT 1 cut(s) 1481
ErhI CCWWGG 4 cut(s) 32, 1164, 1438, 1941
FaqI GGGAC 1 cut(s) 1044
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 5 cut(s) 76, 1422, 1430, 1433, 1764
Fsp4HI GCNGC 5 cut(s) 76, 1422, 1430, 1433, 1764
FspBI CTAG 3 cut(s) 1103, 1128, 1539
GlaI GCGC 1 cut(s) 9
GluI GCNGC 5 cut(s) 76, 1422, 1430, 1433, 1764
GsaI CCCAGC 2 cut(s) 136, 1604
GsuI CTGGAG 1 cut(s) 1529
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 2 cut(s) 1000, 1763
HapII CCGG 3 cut(s) 529, 1094, 1686
HhaI GCGC 1 cut(s) 10
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 5 cut(s) 192, 389, 555, 1267, 1641
HpaII CCGG 3 cut(s) 529, 1094, 1686
HphI GGTGA 2 cut(s) 694, 1127
Hpy166II GTNNAC 6 cut(s) 653, 903, 1115, 1204, 1594, 1780
Hpy8I GTNNAC 6 cut(s) 653, 903, 1115, 1204, 1594, 1780
Hpy99I CGWCG 1 cut(s) 1915
HpyAV CCTTC 6 cut(s) 435, 658, 1031, 1183, 1384, 1618
HpyCH4III ACNGT 6 cut(s) 177, 288, 863, 1080, 1139, 1380
HpyCH4IV ACGT 2 cut(s) 633, 1843
HpyF10VI GCNNNNNNNGC 4 cut(s) 313, 322, 1772, 2000
HpySE526I ACGT 2 cut(s) 633, 1843
HspAI GCGC 1 cut(s) 8
Kpn2I TCCGGA 1 cut(s) 1093
Ksp22I TGATCA 1 cut(s) 24
LmnI GCTCC 6 cut(s) 83, 134, 313, 370, 1324, 1771
Lsp1109I GCAGC 4 cut(s) 62, 1408, 1441, 1444
LweI GCATC 7 cut(s) 437, 496, 1099, 1466, 1488, 1927, 1954
MaeI CTAG 3 cut(s) 1103, 1128, 1539
MaeII ACGT 2 cut(s) 633, 1843
MaeIII GTNAC 4 cut(s) 412, 634, 820, 1976
MbiI CCGCTC 1 cut(s) 1766
MboII GAAGA 9 cut(s) 96, 187, 223, 266, 511, 1361, 1449, 1474, 1933
MfeI CAATTG 2 cut(s) 308, 1770
MflI RGATCY 4 cut(s) 256, 617, 1348, 1688
MhlI GDGCHC 1 cut(s) 655
MlyI GAGTC 3 cut(s) 186, 383, 1276
MmeI TCCRAC 3 cut(s) 595, 1257, 1453
Mph1103I ATGCAT 1 cut(s) 1481
MroI TCCGGA 1 cut(s) 1093
MslI CAYNNNNRTG 4 cut(s) 407, 593, 1821, 1946
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 3 cut(s) 529, 1094, 1686
MspR9I CCNGG 1 cut(s) 1889
MunI CAATTG 2 cut(s) 308, 1770
Mva1269I GAATGC 1 cut(s) 1453
MvaI CCWGG 1 cut(s) 1889
MwoI GCNNNNNNNGC 4 cut(s) 313, 322, 1772, 2000
NcoI CCATGG 1 cut(s) 1941
NlaIV GGNNCC 5 cut(s) 94, 168, 600, 1350, 1762
NmuCI GTSAC 2 cut(s) 634, 1976
NsiI ATGCAT 1 cut(s) 1481
NspI RCATGY 1 cut(s) 1062
PagI TCATGA 1 cut(s) 1717
PctI GAATGC 1 cut(s) 1453
PfeI GAWTC 2 cut(s) 555, 1641
PflMI CCANNNNNTGG 1 cut(s) 1888
PkrI GCNGC 5 cut(s) 77, 1423, 1431, 1434, 1765
PleI GAGTC 3 cut(s) 186, 383, 1275
PpsI GAGTC 3 cut(s) 186, 383, 1275
Ppu21I YACGTR 1 cut(s) 634
PpuMI RGGWCCY 1 cut(s) 93
PshBI ATTAAT 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 1887
PspEI GGTNACC 1 cut(s) 412
PspFI CCCAGC 2 cut(s) 132, 1600
PspGI CCWGG 1 cut(s) 1887
PspN4I GGNNCC 5 cut(s) 94, 168, 600, 1350, 1762
PspPI GGNCC 4 cut(s) 93, 397, 998, 1761
PspPPI RGGWCCY 1 cut(s) 93
PstI CTGCAG 1 cut(s) 498
PsuI RGATCY 4 cut(s) 256, 617, 1348, 1688
RsaI GTAC 2 cut(s) 1042, 1638
RsaNI GTAC 2 cut(s) 1041, 1637
RseI CAYNNNNRTG 4 cut(s) 407, 593, 1821, 1946
SatI GCNGC 5 cut(s) 76, 1422, 1430, 1433, 1764
Sau96I GGNCC 4 cut(s) 93, 397, 998, 1761
SchI GAGTC 3 cut(s) 186, 383, 1276
ScrFI CCNGG 1 cut(s) 1889
SduI GDGCHC 1 cut(s) 655
SfaNI GCATC 7 cut(s) 437, 496, 1099, 1466, 1488, 1927, 1954
SfcI CTRYAG 3 cut(s) 494, 731, 1356
SinI GGWCC 2 cut(s) 93, 397
SmiMI CAYNNNNRTG 4 cut(s) 407, 593, 1821, 1946
SmlI CTYRAG 3 cut(s) 242, 345, 1085
SmoI CTYRAG 3 cut(s) 242, 345, 1085
SsiI CCGC 2 cut(s) 248, 1764
SspI AATATT 1 cut(s) 217
SspMI CTAG 3 cut(s) 1103, 1128, 1539
StyD4I CCNGG 1 cut(s) 1887
StyI CCWWGG 4 cut(s) 32, 1164, 1438, 1941
TaaI ACNGT 6 cut(s) 177, 288, 863, 1080, 1139, 1380
TaiI ACGT 2 cut(s) 636, 1846
TaqI TCGA 2 cut(s) 58, 1913
TauI GCSGC 1 cut(s) 1766
TfiI GAWTC 2 cut(s) 555, 1641
TscAI CASTG 4 cut(s) 25, 609, 1085, 1626
TseFI GTSAC 2 cut(s) 634, 1976
TseI GCWGC 4 cut(s) 75, 1421, 1429, 1432
Tsp45I GTSAC 2 cut(s) 634, 1976
TspGWI ACGGA 2 cut(s) 601, 605
TspRI CASTG 4 cut(s) 25, 609, 1085, 1626
Van91I CCANNNNNTGG 1 cut(s) 1888
Vha464I CTTAAG 1 cut(s) 242
VneI GTGCAC 1 cut(s) 651
VpaK11BI GGWCC 2 cut(s) 93, 397
VspI ATTAAT 1 cut(s) 87
XapI RAATTY 4 cut(s) 60, 183, 789, 2011
XceI RCATGY 1 cut(s) 1062
XcmI CCANNNNNNNNNTGG 1 cut(s) 1171
XspI CTAG 3 cut(s) 1103, 1128, 1539
Zsp2I ATGCAT 1 cut(s) 1481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.