MD17G1023900.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0024435
Physical Location & Seq
Reverse (-)
1757667 .. 1759080
1414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1023900.v1.1.491

Sequence Viewer

Length: 666 bp
ATGAATACCAGCAGATCCGCCCATGCAACCGCCGCCGCCGCCGCCGCTTCGTCTCGGCGTTCAAACCACTGGAAGTACGAGGTGTTCCTCAGCTTAGGCGACGACACACGCACTACCTTCACAGACCACCTCTCCATTGCGTTACGCGACGCCGGAATTAACACGTTTGTAGACTACCAGCTCAGAAGGGAGGAAAATACACCGAGCGAACTTGACGGAGAAATCGAAGGGTCGAGGATCGCCGTCGTCGTCTTCTCAAAGAGGTATGCGGAGGCCGGATGTTGCCTGAGCGAGCTGTCGAAGATCATGAGGTGCCGCGAAGACCAAGAGGGGAAAGTAGTCTATCCAATATTCTACGACGTTGACCCTTCTCAAGTGAGGAAACAGAGCGGTAGTTTTGGGGAAGCATTTCGGAAGCATGAAAGGGATGAAGATCCAGATGAGGTGGAGCAGTGGAGAAGGGACCTTAAGGCTTGTGCAGATTTGTGTGGCCGGAATCTTAAAACCACGGCGGACGGGCGTGAAGGATTGTTTATCCAGAATGTTGTTGGGGACATCATCGCACTAACAAAAACCAGAGACTTACAGACAAGTCAGTCGAGCACTCGGTTGGAATACCTCGTTGCGTGGGCAAGAGTTCCTACTACTGTTTTTTTTTTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

222

Amino Acids

25.04

Weight (kDa)

5.67

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 198 7.6e-41 TIR domain
TIR_2 PF13676 28 - 124 1.2e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024435)

Species Orthologous Gene IDs
malus_domestica MD17G1023900.v1.1 MD17G1024100.v1.1
pyrus_communis pycom111g02260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 595
AccB1I GGYRCC 1 cut(s) 312
AccBSI CCGCTC 1 cut(s) 390
AccI GTMKAC 1 cut(s) 171
AccII CGCG 2 cut(s) 147, 318
AclWI GGATC 3 cut(s) 9, 245, 428
AcoI YGGCCR 1 cut(s) 490
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 1 cut(s) 77
AflII CTTAAG 1 cut(s) 467
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 1 cut(s) 63
AluBI AGCT 3 cut(s) 93, 181, 295
AluI AGCT 3 cut(s) 93, 181, 295
Alw21I GWGCWC 1 cut(s) 605
Alw26I GTCTC 2 cut(s) 57, 573
AlwI GGATC 3 cut(s) 9, 245, 428
AoxI GGCC 2 cut(s) 273, 490
Asp700I GAANNNNTTC 1 cut(s) 408
AspS9I GGNCC 1 cut(s) 463
AvaII GGWCC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 312
BbsI GAAGAC 2 cut(s) 244, 327
Bbv12I GWGCWC 1 cut(s) 605
BbvCI CCTCAGC 1 cut(s) 89
BceAI ACGGC 2 cut(s) 227, 525
BcoDI GTCTC 2 cut(s) 57, 573
BfrI CTTAAG 1 cut(s) 467
BisI GCNGC 6 cut(s) 33, 36, 39, 42, 45, 316
BlsI GCNGC 6 cut(s) 34, 37, 40, 43, 46, 317
Bme18I GGWCC 1 cut(s) 463
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 2 cut(s) 314, 464
BpiI GAAGAC 2 cut(s) 244, 327
Bpu10I CCTNAGC 3 cut(s) 89, 94, 287
BpuEI CTTGAG 1 cut(s) 357
BsaBI GATNNNNATC 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 507
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bse1I ACTGG 1 cut(s) 74
Bse3DI GCAATG 1 cut(s) 135
Bse8I GATNNNNATC 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 2 cut(s) 284, 433
BseJI GATNNNNATC 1 cut(s) 432
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 3 cut(s) 103, 196, 278
BseNI ACTGG 1 cut(s) 74
BsgI GTGCAG 1 cut(s) 498
Bsh1236I CGCG 2 cut(s) 147, 318
BshFI GGCC 2 cut(s) 275, 492
BshNI GGYRCC 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 605
BsiSI CCGG 3 cut(s) 153, 276, 493
BslFI GGGAC 2 cut(s) 476, 566
BsmAI GTCTC 2 cut(s) 57, 573
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 2 cut(s) 476, 566
BsnI GGCC 2 cut(s) 275, 492
Bsp1286I GDGCHC 1 cut(s) 605
Bsp143I GATC 4 cut(s) 14, 237, 303, 433
BspANI GGCC 2 cut(s) 275, 492
BspCNI CTCAG 3 cut(s) 102, 195, 279
BspFNI CGCG 2 cut(s) 147, 318
BspHI TCATGA 1 cut(s) 306
BspLI GGNNCC 2 cut(s) 314, 464
BspPI GGATC 3 cut(s) 9, 245, 428
BspT107I GGYRCC 1 cut(s) 312
BspTI CTTAAG 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 390
BsrDI GCAATG 1 cut(s) 135
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 4 cut(s) 14, 237, 303, 433
BssNI GRCGYC 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 649
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 467
BstC8I GCNNGC 1 cut(s) 293
BstDEI CTNAG 4 cut(s) 89, 94, 182, 287
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 2 cut(s) 284, 433
BstFNI CGCG 2 cut(s) 147, 318
BstKTI GATC 4 cut(s) 17, 240, 306, 436
BstMAI GTCTC 2 cut(s) 57, 573
BstMBI GATC 4 cut(s) 14, 237, 303, 433
BstMWI GCNNNNNNNGC 4 cut(s) 32, 38, 41, 44
BstUI CGCG 2 cut(s) 147, 318
BstV2I GAAGAC 2 cut(s) 244, 327
BstX2I RGATCY 2 cut(s) 14, 433
BstYI RGATCY 2 cut(s) 14, 433
BsuRI GGCC 2 cut(s) 275, 492
BtgI CCRYGG 1 cut(s) 507
BtgZI GCGATG 1 cut(s) 544
BtsCI GGATG 2 cut(s) 284, 433
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 2 cut(s) 67, 458
Cac8I GCNNGC 1 cut(s) 293
CciI TCATGA 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 463
CseI GACGC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 76
CviAII CATG 3 cut(s) 23, 307, 419
CviJI RGCY 6 cut(s) 93, 181, 275, 295, 473, 492
CviKI_1 RGCY 6 cut(s) 93, 181, 275, 295, 473, 492
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 4 cut(s) 89, 94, 182, 287
DpnI GATC 4 cut(s) 16, 239, 305, 435
DpnII GATC 4 cut(s) 14, 237, 303, 433
DrdI GACNNNNNNGTC 1 cut(s) 595
DseDI GACNNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 1 cut(s) 490
EciI GGCGGA 2 cut(s) 7, 527
Eco47I GGWCC 1 cut(s) 463
EcoO109I RGGNCCY 1 cut(s) 463
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 3 cut(s) 26, 310, 422
FaiI YATR 4 cut(s) 24, 267, 308, 420
FaqI GGGAC 2 cut(s) 476, 566
FatI CATG 3 cut(s) 22, 306, 418
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 6 cut(s) 33, 36, 39, 42, 45, 316
FokI GGATG 2 cut(s) 291, 440
Fsp4HI GCNGC 6 cut(s) 33, 36, 39, 42, 45, 316
GluI GCNGC 6 cut(s) 33, 36, 39, 42, 45, 316
HaeIII GGCC 2 cut(s) 275, 492
HapII CCGG 3 cut(s) 153, 276, 493
HgaI GACGC 1 cut(s) 158
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 3 cut(s) 26, 310, 422
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 1 cut(s) 496
HpaII CCGG 3 cut(s) 153, 276, 493
Hpy166II GTNNAC 2 cut(s) 172, 364
Hpy188I TCNGA 2 cut(s) 185, 414
Hpy188III TCNNGA 3 cut(s) 307, 437, 538
Hpy8I GTNNAC 2 cut(s) 172, 364
Hpy99I CGWCG 5 cut(s) 104, 152, 248, 251, 362
HpyAV CCTTC 6 cut(s) 127, 180, 221, 378, 453, 518
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4IV ACGT 2 cut(s) 164, 360
HpyCH4V TGCA 2 cut(s) 26, 479
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 38, 41, 44
HpyF3I CTNAG 4 cut(s) 89, 94, 182, 287
HpySE526I ACGT 2 cut(s) 164, 360
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 3 cut(s) 26, 310, 422
Kzo9I GATC 4 cut(s) 14, 237, 303, 433
LmnI GCTCC 1 cut(s) 448
MaeII ACGT 2 cut(s) 164, 360
MaeIII GTNAC 1 cut(s) 141
MalI GATC 4 cut(s) 16, 239, 305, 435
MbiI CCGCTC 1 cut(s) 390
MboI GATC 4 cut(s) 14, 237, 303, 433
MboII GAAGA 4 cut(s) 244, 313, 332, 443
MflI RGATCY 2 cut(s) 14, 433
MhlI GDGCHC 1 cut(s) 605
MluCI AATT 1 cut(s) 156
MmeI TCCRAC 1 cut(s) 591
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 3 cut(s) 159, 468, 501
MspCI CTTAAG 1 cut(s) 467
MspI CCGG 3 cut(s) 153, 276, 493
MvnI CGCG 2 cut(s) 147, 318
MwoI GCNNNNNNNGC 4 cut(s) 32, 38, 41, 44
NdeII GATC 4 cut(s) 14, 237, 303, 433
NlaIII CATG 3 cut(s) 26, 310, 422
NlaIV GGNNCC 2 cut(s) 314, 464
NmeAIII GCCGAG 1 cut(s) 34
PagI TCATGA 1 cut(s) 306
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 246
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 1 cut(s) 496
PkrI GCNGC 6 cut(s) 34, 37, 40, 43, 46, 317
PpuMI RGGWCCY 1 cut(s) 463
Psp5II RGGWCCY 1 cut(s) 463
PspN4I GGNNCC 2 cut(s) 314, 464
PspPI GGNCC 1 cut(s) 463
PspPPI RGGWCCY 1 cut(s) 463
PsrI GAACNNNNNNTAC 2 cut(s) 68, 100
PsuI RGATCY 2 cut(s) 14, 433
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
SaqAI TTAA 3 cut(s) 159, 468, 501
SatI GCNGC 6 cut(s) 33, 36, 39, 42, 45, 316
Sau3AI GATC 4 cut(s) 14, 237, 303, 433
Sau96I GGNCC 1 cut(s) 463
SduI GDGCHC 1 cut(s) 605
SinI GGWCC 1 cut(s) 463
SmlI CTYRAG 2 cut(s) 372, 467
SmoI CTYRAG 2 cut(s) 372, 467
Sse9I AATT 1 cut(s) 156
SspI AATATT 1 cut(s) 351
TaaI ACNGT 1 cut(s) 649
TaiI ACGT 2 cut(s) 167, 363
TaqI TCGA 4 cut(s) 225, 233, 299, 599
TasI AATT 1 cut(s) 156
TauI GCSGC 6 cut(s) 35, 38, 41, 44, 47, 318
TfiI GAWTC 1 cut(s) 496
Tru1I TTAA 3 cut(s) 159, 468, 501
Tru9I TTAA 3 cut(s) 159, 468, 501
TscAI CASTG 2 cut(s) 74, 458
TspDTI ATGAA 3 cut(s) 17, 435, 444
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 2 cut(s) 74, 458
Vha464I CTTAAG 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.