MD17G1024100.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0024435
Physical Location & Seq
Reverse (-)
1765807 .. 1767539
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1024100.v1.1.491

Sequence Viewer

Length: 729 bp
ATGAATACCAGCAGCCCCGCCCATGCAACCGCCACCGCTGCCGCCACATCGTCTTCCTCTCGGCGTTCAAACCGCTGGAAGTACGAGGTGTTCCTGAGCTTTAGAGGCGAGGACACACGCACTGGGTTCACAGACCACCTCTTCATTGCGTTAGACAGTGCCGGAATCAGCAAGTTTATAGATTACGAGCTCAAAAAGGGGGAGAATATACAGAGAGAACTTGACCGAGCAATCGAAGGGTCGAGGATCGCGGTCGTCGTCTTCTCAAAGAGTTACGCGGAGTCCCGATGGTGCTTGAGGGAGCTGTCGAAGATCATGAGAGAGGGGAAAGTAGTCTATCCTATATTCTACGACGTTGACCCTTCTCAAGTGAGGAATCAGAGCGGTAGTTTTGACGAAGCATTTCGAAAGCATGATGGGGGTGAAGATCCAAATGAGGTGGAGCAGTGGAGAAGGGACCTTAAGGCTTGTGCGGATTTGGGTGGCCGGAGTCTTAAAACCACGGCGGACGGGCGTGAAGGATTGTTTATCCAGAATGTTGTTGGGGACATCATCGCACTAACAAAAACCAGAGACTTACAGACAAGTCAGTCGAGCACTCGGTTGGAATACCTCGTTGCGTGGGCAAGAGTTCCTAGTACTGTTTTTTTTTTTTTTTTTGAAATACCCCCCCTATTTATAACCGAGGAGGGAAGGAGAGATGTCGGTAACTTCTTTCCCATAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

243

Amino Acids

27.42

Weight (kDa)

6.35

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 27 - 196 2.4e-45 TIR domain
TIR_2 PF13676 30 - 122 8.6e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024435)

Species Orthologous Gene IDs
malus_domestica MD17G1023900.v1.1 MD17G1024100.v1.1
pyrus_communis pycom111g02260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 680
AasI GACNNNNNNGTC 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 384
AccII CGCG 2 cut(s) 251, 278
AclWI GGATC 2 cut(s) 254, 422
AcoI YGGCCR 1 cut(s) 484
AfaI GTAC 2 cut(s) 83, 640
AflII CTTAAG 1 cut(s) 461
AgsI TTSAA 2 cut(s) 69, 662
AluBI AGCT 3 cut(s) 99, 190, 304
AluI AGCT 3 cut(s) 99, 190, 304
Alw21I GWGCWC 2 cut(s) 192, 599
Alw26I GTCTC 1 cut(s) 567
AlwI GGATC 2 cut(s) 254, 422
AoxI GGCC 1 cut(s) 484
ApeKI GCWGC 2 cut(s) 12, 38
Asp700I GAANNNNTTC 1 cut(s) 402
AspS9I GGNCC 1 cut(s) 457
AsuHPI GGTGA 1 cut(s) 434
AsuII TTCGAA 1 cut(s) 406
AvaII GGWCC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 192
BbsI GAAGAC 2 cut(s) 45, 253
Bbv12I GWGCWC 2 cut(s) 192, 599
BbvI GCAGC 2 cut(s) 24, 25
BccI CCATC 2 cut(s) 282, 410
BceAI ACGGC 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 2 cut(s) 636, 727
BfrI CTTAAG 1 cut(s) 461
BisI GCNGC 3 cut(s) 13, 39, 42
BlsI GCNGC 3 cut(s) 14, 40, 43
BmcAI AGTACT 1 cut(s) 640
Bme18I GGWCC 1 cut(s) 457
BmgT120I GGNCC 1 cut(s) 457
BmiI GGNNCC 1 cut(s) 458
BmrI ACTGGG 1 cut(s) 132
BmuI ACTGGG 1 cut(s) 132
BpiI GAAGAC 2 cut(s) 45, 253
Bpu10I CCTNAGC 1 cut(s) 95
Bpu14I TTCGAA 1 cut(s) 406
BpuEI CTTGAG 2 cut(s) 316, 351
BsaJI CCNNGG 2 cut(s) 501, 684
Bse1I ACTGG 1 cut(s) 127
Bse3DI GCAATG 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 501, 684
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 1 cut(s) 127
BseRI GAGGAG 1 cut(s) 701
BseXI GCAGC 2 cut(s) 24, 25
Bsh1236I CGCG 2 cut(s) 251, 278
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 486
BsiEI CGRYCG 1 cut(s) 255
BsiHKAI GWGCWC 2 cut(s) 192, 599
BsiSI CCGG 2 cut(s) 162, 487
BslFI GGGAC 3 cut(s) 268, 470, 560
BsmAI GTCTC 1 cut(s) 567
BsmFI GGGAC 3 cut(s) 268, 470, 560
BsnI GGCC 1 cut(s) 486
Bsp119I TTCGAA 1 cut(s) 406
Bsp1286I GDGCHC 2 cut(s) 192, 599
Bsp143I GATC 3 cut(s) 246, 312, 427
BspANI GGCC 1 cut(s) 486
BspCNI CTCAG 1 cut(s) 87
BspFNI CGCG 2 cut(s) 251, 278
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 458
BspPI GGATC 2 cut(s) 254, 422
BspT104I TTCGAA 1 cut(s) 406
BspTI CTTAAG 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 1 cut(s) 127
BssECI CCNNGG 2 cut(s) 501, 684
BssMI GATC 3 cut(s) 246, 312, 427
Bst4CI ACNGT 2 cut(s) 158, 643
Bst6I CTCTTC 1 cut(s) 146
BstAFI CTTAAG 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 406
BstDEI CTNAG 1 cut(s) 95
BstDSI CCRYGG 1 cut(s) 501
BstFNI CGCG 2 cut(s) 251, 278
BstKTI GATC 3 cut(s) 249, 315, 430
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 3 cut(s) 246, 312, 427
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 2 cut(s) 38, 105
BstUI CGCG 2 cut(s) 251, 278
BstV1I GCAGC 2 cut(s) 24, 25
BstV2I GAAGAC 2 cut(s) 45, 253
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 1 cut(s) 486
BtgI CCRYGG 1 cut(s) 501
BtgZI GCGATG 1 cut(s) 538
BtsI GCAGTG 1 cut(s) 452
BtsIMutI CAGTG 3 cut(s) 120, 163, 452
CciI TCATGA 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 457
Csp6I GTAC 2 cut(s) 82, 639
CspCI CAANNNNNGTGG 2 cut(s) 420, 455
CviAII CATG 3 cut(s) 23, 316, 413
CviJI RGCY 7 cut(s) 15, 99, 190, 304, 467, 486, 725
CviKI_1 RGCY 7 cut(s) 15, 99, 190, 304, 467, 486, 725
CviQI GTAC 2 cut(s) 82, 639
DdeI CTNAG 1 cut(s) 95
DpnI GATC 3 cut(s) 248, 314, 429
DpnII GATC 3 cut(s) 246, 312, 427
DrdI GACNNNNNNGTC 1 cut(s) 589
DseDI GACNNNNNNGTC 1 cut(s) 589
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
EciI GGCGGA 1 cut(s) 521
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 457
Eco53kI GAGCTC 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 3 cut(s) 26, 319, 416
FaiI YATR 8 cut(s) 24, 179, 209, 317, 344, 414, 680, 722
FaqI GGGAC 3 cut(s) 268, 470, 560
FatI CATG 3 cut(s) 22, 315, 412
FauI CCCGC 1 cut(s) 25
Fnu4HI GCNGC 3 cut(s) 13, 39, 42
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 3 cut(s) 13, 39, 42
FspBI CTAG 2 cut(s) 636, 727
GluI GCNGC 3 cut(s) 13, 39, 42
HaeIII GGCC 1 cut(s) 486
HapII CCGG 2 cut(s) 162, 487
Hin1II CATG 3 cut(s) 26, 319, 416
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 4 cut(s) 165, 281, 376, 490
HpaII CCGG 2 cut(s) 162, 487
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 129, 358
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 4 cut(s) 94, 285, 316, 532
Hpy8I GTNNAC 2 cut(s) 129, 358
Hpy99I CGWCG 2 cut(s) 260, 356
HpyAV CCTTC 5 cut(s) 230, 372, 447, 512, 687
HpyCH4III ACNGT 2 cut(s) 158, 643
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 1 cut(s) 26
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 105
HpyF3I CTNAG 1 cut(s) 95
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 3 cut(s) 26, 319, 416
Kzo9I GATC 3 cut(s) 246, 312, 427
LmnI GCTCC 2 cut(s) 301, 442
LpnPI CCDG 8 cut(s) 22, 61, 107, 108, 175, 500, 545, 583
Lsp1109I GCAGC 2 cut(s) 24, 25
MaeI CTAG 2 cut(s) 636, 727
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 2 cut(s) 272, 707
MalI GATC 3 cut(s) 248, 314, 429
MbiI CCGCTC 1 cut(s) 384
MboI GATC 3 cut(s) 246, 312, 427
MboII GAAGA 5 cut(s) 45, 133, 253, 322, 437
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 2 cut(s) 192, 599
MlyI GAGTC 2 cut(s) 290, 499
MmeI TCCRAC 1 cut(s) 585
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 2 cut(s) 462, 495
MspA1I CMGCKG 2 cut(s) 38, 75
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 2 cut(s) 162, 487
MvnI CGCG 2 cut(s) 251, 278
MwoI GCNNNNNNNGC 2 cut(s) 38, 105
NdeII GATC 3 cut(s) 246, 312, 427
NlaIII CATG 3 cut(s) 26, 319, 416
NlaIV GGNNCC 1 cut(s) 458
NmeAIII GCCGAG 1 cut(s) 40
NspV TTCGAA 1 cut(s) 406
PagI TCATGA 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 165, 376
PkrI GCNGC 3 cut(s) 14, 40, 43
PleI GAGTC 2 cut(s) 289, 498
PpsI GAGTC 2 cut(s) 289, 498
PpuMI RGGWCCY 1 cut(s) 457
PsiI TTATAA 1 cut(s) 680
Psp124BI GAGCTC 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 457
PspN4I GGNNCC 1 cut(s) 458
PspPI GGNCC 1 cut(s) 457
PspPPI RGGWCCY 1 cut(s) 457
PsrI GAACNNNNNNTAC 2 cut(s) 74, 106
PsuI RGATCY 1 cut(s) 427
RsaI GTAC 2 cut(s) 83, 640
RsaNI GTAC 2 cut(s) 82, 639
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 2 cut(s) 462, 495
SatI GCNGC 3 cut(s) 13, 39, 42
Sau3AI GATC 3 cut(s) 246, 312, 427
Sau96I GGNCC 1 cut(s) 457
ScaI AGTACT 1 cut(s) 640
SchI GAGTC 2 cut(s) 290, 499
SduI GDGCHC 2 cut(s) 192, 599
SetI ASST 9 cut(s) 90, 101, 141, 192, 306, 357, 441, 462, 615
SfuI TTCGAA 1 cut(s) 406
SinI GGWCC 1 cut(s) 457
SmlI CTYRAG 3 cut(s) 295, 366, 461
SmoI CTYRAG 3 cut(s) 295, 366, 461
SspMI CTAG 2 cut(s) 636, 727
SstI GAGCTC 1 cut(s) 192
TaaI ACNGT 2 cut(s) 158, 643
TaiI ACGT 1 cut(s) 357
TaqI TCGA 5 cut(s) 234, 242, 308, 406, 593
TaqII GACCGA 1 cut(s) 240
TatI WGTACW 1 cut(s) 638
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 2 cut(s) 165, 376
Tru1I TTAA 2 cut(s) 462, 495
Tru9I TTAA 2 cut(s) 462, 495
TscAI CASTG 3 cut(s) 127, 163, 452
TseI GCWGC 2 cut(s) 12, 38
TspDTI ATGAA 2 cut(s) 17, 133
TspRI CASTG 3 cut(s) 127, 163, 452
Vha464I CTTAAG 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 457
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 2 cut(s) 636, 727
ZrmI AGTACT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.