Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0016571

Physical Location & Seq

Genomic Coordinates Forward (+)

4224712 .. 4226529

Gene Structure 1818 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1054700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGCAAATGTGTGTACCAGTTCGATCCCCGTCGTGGGTTTCCCGGCGACGGATCTTAGACCAGAAGCTCTCCGACCTCCACAGATGTACAAACCTCAGCCACGTCAAGCAAGTCCACGCCCAAATCCTCAAAGCCGACCTGCACCAGGACATCCACACGGCGCCCAAGCTCATCGCCGCTTTCTCCCTCTGCCGGCAGATGGCGCTGGCGGTAAACGTTTTCAATCAGATTGATTACCCAAATGTCCACTTGTACAACACTCTTATCAGAGCTCACATTCAGAACTCGCAGACTTCTCAGGCCTTCGCGGCCTTCTTCGATATGCAGATTAATGGGGTTTATCCCGATAATTTTACTTACCCATTTCTTCTGAAAGCTTGTTTGGGCCGGCCATGGCTGCCGGTAGTTCAAATGATTCATGCCCACATTGAGAAATTTGGATTTGGGTTGGATATCTTCGTGCCTAATTCGCTCATTGATACTTATTCAAAGTGTGGATTGATTGGTGTTAGCGAGGCGAAGAAGCTGTTTGTGGTGATGGAAGAGAGAGATACTGTGTCTTGGAATTCAATGATTGGTGGGTTGGCAAAAGCTGGGGAGCTGAGTGATGCACGCCGCTTGTTCGAAGAAATGCCTGGGAGAGATTCGGTTAGTTGGAATACGATATTGGATGGGTATGTGAAGGCTGGGGAGATGAATGAAGCCTTTGAATTGTTTGAGAAAATGCCTCAGAGGAATGTTGTCTCGTGGTCGACTCTGGTTTCTGGTTATAGCAAAGCTGGGGATATGAGTATGGCAAGAATGATGTTTGATAGGATGCCCTTCAGGAACTTGGTTCCATGGACTATTATTATATCTGGTTATGCCGAGAAGGGGCTTGCGAAGGAGGCGATAATGCTATATGATCAAATGGAGGAAGCTGGATTGAAGCCTGATAATGGTGCTGTTATTAGTATATTGGCCGCTTGTGCAGAGTCTGGTTTGATTGGATTGGGTAGGAAAGTTCATGCCTCAATTGAGAGGACTCAGTTCAAGTGTAGTACTCCTGTGTCGAATGCTTTGCTTGACATGTATGCCAAGTGTGGTGTGTTGGATGAAGCATTTAGAGTCTTTGATGGGATAGCAAAAAAAGATTTGGTGTCTTGGAATGCCATGCTCCAAGGGTTAGCCATGCACGGGCATGGCGAGAAGGCTCTTGAGCTTTTCTCTCGAATGGTGAAAGATGGGTTTTTTCCGGATAAAGTTACCTTCATTGGGGTCTTATGTGCTTGTACACATATAGGATTTGTGGAGGAAGGTCTTCACGCTTTCAACACGATGGAGAGGGAGTATGGGATTGTACCTGAAGTTGAGCATTATGGTTGCATGATTGACCTTTTGGGTCGTGGTGGGCATCTCCAGGAGGCTTTTAGGCTTGTGCATAGCATGCCAATGGAACCGAATGCTGTTATATGGGGTACCCTTTTGGGGGCATGTCGGATGCATAATGATCGGGAACTAGCAGAGGAGGTGCTTAATCACCTAGTTAAGTTAGACCCGTCTGAACCAGGAAACTTTTCCATGTTGTCAAATATCTATGCTGCAGCTGGTGACTGGGCAAATGTTGCTAATGTAAGGATGCAAATGAGGAGCACCGGAGTCCAGAAACCTTCTGGGGCTAGTTCCATCGAGGTGGACGACGAGGTCCATGAATTTACAGTTTTTGATAAATTACACCCTAAATCTGACGAAGTGTATGGGATGATCGACAGACTTCGTGTGGATTTCAAGCAATTGAATGTGTCATCGCGGAGTATTAGCAATGGGATAGGTGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

606

Amino Acids

67.54

Weight (kDa)

6.1

Isoelectric Point (pI)

43.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	80 - 127	7.3e-11	PPR repeat family
PPR_2	PF13041	183 - 212	1.5e-06	PPR repeat family
PPR_1	PF12854	183 - 211	3.7e-07	PPR repeat
PPR	PF01535	186 - 214	1e-07	PPR repeat
PPR_2	PF13041	215 - 247	2.6e-10	PPR repeat family
PPR_1	PF12854	215 - 242	9.4e-08	PPR repeat
PPR	PF01535	217 - 247	5.4e-09	PPR repeat
PPR	PF01535	248 - 275	1.5e-06	PPR repeat
PPR_2	PF13041	277 - 323	8.3e-08	PPR repeat family
PPR	PF01535	280 - 309	4.6e-06	PPR repeat
PPR_2	PF13041	378 - 424	2.2e-11	PPR repeat family
PPR	PF01535	380 - 410	3.5e-08	PPR repeat
E_motif	PF20431	495 - 557	2.6e-17	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016571)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29230
fragaria_vesca	FvH4_6g47760
malus_domestica	MD17G1054700.v1.1
prunus_persica	Prupe.3G261400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0166911
rosa_laevigata	RLG00000021678
rosa_multiflora	Rmu_sc0002735.1_g000016 Rmu_sc0005344.1_g000009
rosa_roxburghii	Rroxscaffold_2G00084020
rosa_rugosa	Rorug02G0526300
rosa_samantha	Rh2BG604800 Rh2CG575500 Rh2DG616400
rosa_wichuraiana	Rw2G049390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1682
Acc36I	ACCTGC	1 cut(s)	147
Acc65I	GGTACC	1 cut(s)	1457
AccB1I	GGYRCC	2 cut(s)	160, 1457
AccI	GTMKAC	1 cut(s)	752
AccII	CGCG	2 cut(s)	308, 1789
AccIII	TCCGGA	1 cut(s)	1234
AciI	CCGC	6 cut(s)	177, 209, 308, 616, 963, 1789
AclI	AACGTT	1 cut(s)	216
AclWI	GGATC	2 cut(s)	18, 59
AcoI	YGGCCR	2 cut(s)	389, 960
AcsI	RAATTY	3 cut(s)	434, 565, 1691
AcuI	CTGAAG	2 cut(s)	808, 1365
AcyI	GRCGYC	1 cut(s)	161
AfaI	GTAC	7 cut(s)	15, 88, 254, 1042, 1273, 1341, 1459
AflIII	ACRYGT	1 cut(s)	1068
AjiI	CACGTC	1 cut(s)	103
AjnI	CCWGG	4 cut(s)	144, 634, 1398, 1546
AjuI	GAANNNNNNNTTGG	4 cut(s)	365, 397, 650, 682
Alw21I	GWGCWC	2 cut(s)	274, 1634
Alw26I	GTCTC	1 cut(s)	748
AlwI	GGATC	2 cut(s)	18, 59
AlwNI	CAGNNNCTG	1 cut(s)	977
Aor13HI	TCCGGA	1 cut(s)	1234
AoxI	GGCC	5 cut(s)	300, 309, 385, 389, 960
ApeKI	GCWGC	3 cut(s)	397, 1580, 1583
ApoI	RAATTY	3 cut(s)	434, 565, 1691
AseI	ATTAAT	1 cut(s)	330
Asp700I	GAANNNNTTC	2 cut(s)	1299, 1555
Asp718I	GGTACC	1 cut(s)	1457
AspLEI	GCGC	2 cut(s)	163, 205
AspS9I	GGNCC	2 cut(s)	385, 1684
AsuC2I	CCSGG	1 cut(s)	43
AsuHPI	GGTGA	4 cut(s)	547, 1228, 1511, 1601
AsuII	TTCGAA	1 cut(s)	624
AvaII	GGWCC	1 cut(s)	1684
BanI	GGYRCC	2 cut(s)	160, 1457
BanII	GRGCYC	1 cut(s)	274
BauI	CACGAG	1 cut(s)	745
BbsI	GAAGAC	1 cut(s)	1292
Bbv12I	GWGCWC	2 cut(s)	274, 1634
BbvCI	CCTCAGC	1 cut(s)	95
BbvI	GCAGC	3 cut(s)	384, 1567, 1595
BccI	CCATC	7 cut(s)	193, 532, 665, 1109, 1217, 1312, 1673
BceAI	ACGGC	1 cut(s)	174
BcgI	CGANNNNNNTGC	4 cut(s)	1042, 1076, 1472, 1506
BciT130I	CCWGG	4 cut(s)	146, 636, 1400, 1548
BclI	TGATCA	1 cut(s)	904
BcnI	CCSGG	1 cut(s)	43
BcoDI	GTCTC	1 cut(s)	748
BfaI	CTAG	3 cut(s)	1499, 1523, 1659
BfmI	CTRYAG	1 cut(s)	1581
BfoI	RGCGCY	2 cut(s)	164, 206
BfuAI	ACCTGC	1 cut(s)	147
BglI	GCCNNNNNGGC	1 cut(s)	308
BisI	GCNGC	7 cut(s)	177, 309, 398, 616, 963, 1581, 1584
BlsI	GCNGC	7 cut(s)	178, 310, 399, 617, 964, 1582, 1585
BmcAI	AGTACT	1 cut(s)	1042
Bme1390I	CCNGG	5 cut(s)	43, 146, 636, 1400, 1548
Bme18I	GGWCC	1 cut(s)	1684
BmgBI	CACGTC	1 cut(s)	103
BmgT120I	GGNCC	2 cut(s)	385, 1684
BmiI	GGNNCC	4 cut(s)	162, 837, 1437, 1459
BmrFI	CCNGG	5 cut(s)	43, 146, 636, 1400, 1548
BmrI	ACTGGG	1 cut(s)	1603
BmsI	GCATC	5 cut(s)	598, 807, 1402, 1470, 1608
BmuI	ACTGGG	1 cut(s)	1603
BpiI	GAAGAC	1 cut(s)	1292
BplI	GAGNNNNNCTC	2 cut(s)	1190, 1222
BpmI	CTGGAG	1 cut(s)	1382
Bpu10I	CCTNAGC	1 cut(s)	95
Bpu14I	TTCGAA	1 cut(s)	624
BpuEI	CTTGAG	1 cut(s)	1217
BpuMI	CCSGG	1 cut(s)	43
BsaHI	GRCGYC	1 cut(s)	161
BsaJI	CCNNGG	4 cut(s)	392, 635, 839, 1159
BsaWI	WCCGGW	2 cut(s)	1234, 1634
BsaXI	ACNNNNNCTCC	2 cut(s)	1313, 1343
Bse118I	RCCGGY	3 cut(s)	192, 387, 400
Bse1I	ACTGG	2 cut(s)	17, 1598
Bse3DI	GCAATG	1 cut(s)	1807
BseAI	TCCGGA	1 cut(s)	1234
BseBI	CCWGG	4 cut(s)	146, 636, 1400, 1548
BseDI	CCNNGG	4 cut(s)	392, 635, 839, 1159
BseGI	GGATG	7 cut(s)	150, 676, 822, 1099, 1485, 1623, 1746
BseMI	GCAATG	1 cut(s)	1807
BseMII	CTCAG	5 cut(s)	109, 311, 593, 743, 1040
BseNI	ACTGG	2 cut(s)	17, 1598
BseRI	GAGGAG	2 cut(s)	1520, 1642
BseXI	GCAGC	3 cut(s)	384, 1567, 1595
BseYI	CCCAGC	3 cut(s)	593, 686, 779
BsgI	GTGCAG	2 cut(s)	125, 990
Bsh1236I	CGCG	2 cut(s)	308, 1789
BshFI	GGCC	5 cut(s)	302, 311, 387, 391, 962
BshNI	GGYRCC	2 cut(s)	160, 1457
BsiHKAI	GWGCWC	2 cut(s)	274, 1634
BsiSI	CCGG	6 cut(s)	43, 193, 388, 401, 1235, 1635
BsmAI	GTCTC	1 cut(s)	748
BsmI	GAATGC	3 cut(s)	1060, 1153, 1447
BsnI	GGCC	5 cut(s)	302, 311, 387, 391, 962
Bsp119I	TTCGAA	1 cut(s)	624
Bsp1286I	GDGCHC	2 cut(s)	274, 1634
Bsp13I	TCCGGA	1 cut(s)	1234
Bsp1407I	TGTACA	3 cut(s)	86, 252, 1271
Bsp143I	GATC	5 cut(s)	23, 51, 904, 1489, 1743
Bsp19I	CCATGG	2 cut(s)	392, 839
BspACI	CCGC	6 cut(s)	177, 209, 308, 616, 963, 1789
BspANI	GGCC	5 cut(s)	302, 311, 387, 391, 962
BspCNI	CTCAG	5 cut(s)	108, 310, 594, 742, 1039
BspEI	TCCGGA	1 cut(s)	1234
BspFNI	CGCG	2 cut(s)	308, 1789
BspLI	GGNNCC	4 cut(s)	162, 837, 1437, 1459
BspMAI	CTGCAG	1 cut(s)	1585
BspMI	ACCTGC	1 cut(s)	147
BspPI	GGATC	2 cut(s)	18, 59
BspT104I	TTCGAA	1 cut(s)	624
BspT107I	GGYRCC	2 cut(s)	160, 1457
BsrDI	GCAATG	1 cut(s)	1807
BsrFI	RCCGGY	3 cut(s)	192, 387, 400
BsrGI	TGTACA	3 cut(s)	86, 252, 1271
BsrI	ACTGG	2 cut(s)	17, 1598
BssAI	RCCGGY	3 cut(s)	192, 387, 400
BssECI	CCNNGG	4 cut(s)	392, 635, 839, 1159
BssMI	GATC	5 cut(s)	23, 51, 904, 1489, 1743
BssNI	GRCGYC	1 cut(s)	161
BssSI	CACGAG	1 cut(s)	745
BssT1I	CCWWGG	3 cut(s)	392, 839, 1159
Bst2BI	CACGAG	1 cut(s)	745
Bst2UI	CCWGG	4 cut(s)	146, 636, 1400, 1548
Bst4CI	ACNGT	2 cut(s)	556, 1699
Bst6I	CTCTTC	1 cut(s)	537
BstACI	GRCGYC	1 cut(s)	161
BstAPI	GCANNNNNTGC	2 cut(s)	1426, 1604
BstAUI	TGTACA	3 cut(s)	86, 252, 1271
BstBI	TTCGAA	1 cut(s)	624
BstC8I	GCNNGC	6 cut(s)	194, 207, 389, 613, 879, 1427
BstDEI	CTNAG	6 cut(s)	55, 95, 297, 602, 729, 1026
BstDSI	CCRYGG	2 cut(s)	392, 839
BstENI	CCTNNNNNAGG	1 cut(s)	143
BstF5I	GGATG	7 cut(s)	150, 676, 822, 1099, 1485, 1623, 1746
BstFNI	CGCG	2 cut(s)	308, 1789
BstH2I	RGCGCY	2 cut(s)	164, 206
BstHHI	GCGC	2 cut(s)	163, 205
BstKTI	GATC	5 cut(s)	26, 54, 907, 1492, 1746
BstMAI	GTCTC	1 cut(s)	748
BstMBI	GATC	5 cut(s)	23, 51, 904, 1489, 1743
BstMWI	GCNNNNNNNGC	8 cut(s)	202, 308, 397, 469, 887, 968, 1426, 1604
BstNI	CCWGG	4 cut(s)	146, 636, 1400, 1548
BstNSI	RCATGY	3 cut(s)	1072, 1429, 1476
BstSCI	CCNGG	5 cut(s)	41, 144, 634, 1398, 1546
BstSFI	CTRYAG	1 cut(s)	1581
BstUI	CGCG	2 cut(s)	308, 1789
BstV1I	GCAGC	3 cut(s)	384, 1567, 1595
BstV2I	GAAGAC	1 cut(s)	1292
BstX2I	RGATCY	1 cut(s)	51
BstXI	CCANNNNNNTGG	1 cut(s)	1672
BstYI	RGATCY	1 cut(s)	51
BsuRI	GGCC	5 cut(s)	302, 311, 387, 391, 962
BtgI	CCRYGG	2 cut(s)	392, 839
BtgZI	GCGATG	2 cut(s)	157, 1770
BtrI	CACGTC	1 cut(s)	103
BtsCI	GGATG	7 cut(s)	150, 676, 822, 1099, 1485, 1623, 1746
BveI	ACCTGC	1 cut(s)	147
Cac8I	GCNNGC	6 cut(s)	194, 207, 389, 613, 879, 1427
CaiI	CAGNNNCTG	1 cut(s)	977
CfoI	GCGC	2 cut(s)	163, 205
Cfr10I	RCCGGY	3 cut(s)	192, 387, 400
Cfr13I	GGNCC	2 cut(s)	385, 1684
Csp6I	GTAC	7 cut(s)	14, 87, 253, 1041, 1272, 1340, 1458
CviQI	GTAC	7 cut(s)	14, 87, 253, 1041, 1272, 1340, 1458
DdeI	CTNAG	6 cut(s)	55, 95, 297, 602, 729, 1026
DinI	GGCGCC	1 cut(s)	162
DpnI	GATC	5 cut(s)	25, 53, 906, 1491, 1745
DpnII	GATC	5 cut(s)	23, 51, 904, 1489, 1743
DrdI	GACNNNNNNGTC	1 cut(s)	1682
DseDI	GACNNNNNNGTC	1 cut(s)	1682
EaeI	YGGCCR	2 cut(s)	389, 960
Eam1104I	CTCTTC	1 cut(s)	537
EarI	CTCTTC	1 cut(s)	537
Ecl136II	GAGCTC	1 cut(s)	272
Eco130I	CCWWGG	3 cut(s)	392, 839, 1159
Eco147I	AGGCCT	1 cut(s)	302
Eco24I	GRGCYC	1 cut(s)	274
Eco32I	GATATC	1 cut(s)	454
Eco47I	GGWCC	1 cut(s)	1684
Eco53kI	GAGCTC	1 cut(s)	272
Eco57I	CTGAAG	2 cut(s)	808, 1365
EcoICRI	GAGCTC	1 cut(s)	272
EcoNI	CCTNNNNNAGG	1 cut(s)	143
EcoRI	GAATTC	1 cut(s)	565
EcoRII	CCWGG	4 cut(s)	144, 634, 1398, 1546
EcoRV	GATATC	1 cut(s)	454
EcoT14I	CCWWGG	3 cut(s)	392, 839, 1159
EcoT22I	ATGCAT	1 cut(s)	1485
EcoT38I	GRGCYC	1 cut(s)	274
EgeI	GGCGCC	1 cut(s)	162
EheI	GGCGCC	1 cut(s)	162
ErhI	CCWWGG	3 cut(s)	392, 839, 1159
FbaI	TGATCA	1 cut(s)	904
FblI	GTMKAC	1 cut(s)	752
Fnu4HI	GCNGC	7 cut(s)	177, 309, 398, 616, 963, 1581, 1584
FokI	GGATG	7 cut(s)	137, 683, 829, 1106, 1492, 1630, 1753
FriOI	GRGCYC	1 cut(s)	274
FseI	GGCCGGCC	1 cut(s)	391
Fsp4HI	GCNGC	7 cut(s)	177, 309, 398, 616, 963, 1581, 1584
FspBI	CTAG	3 cut(s)	1499, 1523, 1659
GlaI	GCGC	2 cut(s)	162, 204
GluI	GCNGC	7 cut(s)	177, 309, 398, 616, 963, 1581, 1584
GsaI	CCCAGC	3 cut(s)	597, 690, 783
GsuI	CTGGAG	1 cut(s)	1382
HaeII	RGCGCY	2 cut(s)	164, 206
HaeIII	GGCC	5 cut(s)	302, 311, 387, 391, 962
HapII	CCGG	6 cut(s)	43, 193, 388, 401, 1235, 1635
HhaI	GCGC	2 cut(s)	163, 205
Hin1I	GRCGYC	1 cut(s)	161
Hin6I	GCGC	2 cut(s)	161, 203
HinP1I	GCGC	2 cut(s)	161, 203
HincII	GTYRAC	1 cut(s)	753
HindII	GTYRAC	1 cut(s)	753
HindIII	AAGCTT	1 cut(s)	375
HinfI	GANTC	7 cut(s)	415, 644, 754, 974, 1024, 1107, 1638
HpaII	CCGG	6 cut(s)	43, 193, 388, 401, 1235, 1635
HphI	GGTGA	4 cut(s)	547, 1228, 1511, 1601
Hpy166II	GTNNAC	7 cut(s)	14, 115, 214, 247, 753, 1274, 1675
Hpy188I	TCNGA	9 cut(s)	73, 228, 269, 282, 372, 732, 1479, 1543, 1726
Hpy188III	TCNNGA	7 cut(s)	344, 826, 1196, 1209, 1235, 1493, 1642
Hpy8I	GTNNAC	7 cut(s)	14, 115, 214, 247, 753, 1274, 1675
Hpy99I	CGWCG	3 cut(s)	34, 51, 1682
HpyCH4III	ACNGT	2 cut(s)	556, 1699
HpyCH4IV	ACGT	2 cut(s)	102, 216
HpyF10VI	GCNNNNNNNGC	8 cut(s)	202, 308, 397, 469, 887, 968, 1426, 1604
HpyF3I	CTNAG	6 cut(s)	55, 95, 297, 602, 729, 1026
HpySE526I	ACGT	2 cut(s)	102, 216
Hsp92I	GRCGYC	1 cut(s)	161
HspAI	GCGC	2 cut(s)	161, 203
KasI	GGCGCC	1 cut(s)	160
Kpn2I	TCCGGA	1 cut(s)	1234
KpnI	GGTACC	1 cut(s)	1461
KroI	GCCGGC	2 cut(s)	192, 387
KroNI	GCCGGC	2 cut(s)	194, 389
Ksp22I	TGATCA	1 cut(s)	904
Kzo9I	GATC	5 cut(s)	23, 51, 904, 1489, 1743
LmnI	GCTCC	3 cut(s)	598, 1161, 1629
Lsp1109I	GCAGC	3 cut(s)	384, 1567, 1595
LweI	GCATC	5 cut(s)	598, 807, 1402, 1470, 1608
MaeI	CTAG	3 cut(s)	1499, 1523, 1659
MaeII	ACGT	2 cut(s)	102, 216
MaeIII	GTNAC	2 cut(s)	1243, 1589
MalI	GATC	5 cut(s)	25, 53, 906, 1491, 1745
MboI	GATC	5 cut(s)	23, 51, 904, 1489, 1743
MboII	GAAGA	7 cut(s)	307, 359, 448, 532, 554, 638, 1292
MfeI	CAATTG	2 cut(s)	1014, 1772
MflI	RGATCY	1 cut(s)	51
MhlI	GDGCHC	2 cut(s)	274, 1634
MluCI	AATT	9 cut(s)	349, 434, 466, 565, 710, 1014, 1691, 1709, 1772
Mly113I	GGCGCC	1 cut(s)	161
MlyI	GAGTC	5 cut(s)	748, 983, 1018, 1116, 1647
MmeI	TCCRAC	5 cut(s)	96, 429, 635, 1071, 1457
Mph1103I	ATGCAT	1 cut(s)	1485
MroI	TCCGGA	1 cut(s)	1234
MroNI	GCCGGC	2 cut(s)	192, 387
MroXI	GAANNNNTTC	2 cut(s)	1299, 1555
MseI	TTAA	3 cut(s)	330, 1515, 1527
MslI	CAYNNNNRTG	3 cut(s)	1179, 1430, 1670
MspA1I	CMGCKG	1 cut(s)	1586
MspI	CCGG	6 cut(s)	43, 193, 388, 401, 1235, 1635
MspR9I	CCNGG	5 cut(s)	43, 146, 636, 1400, 1548
MunI	CAATTG	2 cut(s)	1014, 1772
Mva1269I	GAATGC	3 cut(s)	1060, 1153, 1447
MvaI	CCWGG	4 cut(s)	146, 636, 1400, 1548
MvnI	CGCG	2 cut(s)	308, 1789
MwoI	GCNNNNNNNGC	8 cut(s)	202, 308, 397, 469, 887, 968, 1426, 1604
NaeI	GCCGGC	2 cut(s)	194, 389
NarI	GGCGCC	1 cut(s)	161
NciI	CCSGG	1 cut(s)	43
NcoI	CCATGG	2 cut(s)	392, 839
NdeII	GATC	5 cut(s)	23, 51, 904, 1489, 1743
NgoMIV	GCCGGC	2 cut(s)	192, 387
NlaIV	GGNNCC	4 cut(s)	162, 837, 1437, 1459
NmeAIII	GCCGAG	1 cut(s)	892
NmuCI	GTSAC	1 cut(s)	1589
NsiI	ATGCAT	1 cut(s)	1485
NspI	RCATGY	3 cut(s)	1072, 1429, 1476
NspV	TTCGAA	1 cut(s)	624
PaeI	GCATGC	1 cut(s)	1429
PceI	AGGCCT	1 cut(s)	302
PciI	ACATGT	1 cut(s)	1068
PcsI	WCGNNNNNNNCGW	2 cut(s)	1182, 1674
PctI	GAATGC	3 cut(s)	1060, 1153, 1447
PdiI	GCCGGC	2 cut(s)	194, 389
PdmI	GAANNNNTTC	2 cut(s)	1299, 1555
PfeI	GAWTC	2 cut(s)	415, 644
PflFI	GACNNNGTC	1 cut(s)	1682
PfoI	TCCNGGA	1 cut(s)	1398
PkrI	GCNGC	7 cut(s)	178, 310, 399, 617, 964, 1582, 1585
PleI	GAGTC	5 cut(s)	748, 982, 1018, 1115, 1646
PluTI	GGCGCC	1 cut(s)	164
PpsI	GAGTC	5 cut(s)	748, 982, 1018, 1115, 1646
PscI	ACATGT	1 cut(s)	1068
PshBI	ATTAAT	1 cut(s)	330
Psp124BI	GAGCTC	1 cut(s)	274
Psp1406I	AACGTT	1 cut(s)	216
Psp6I	CCWGG	4 cut(s)	144, 634, 1398, 1546
PspFI	CCCAGC	3 cut(s)	593, 686, 779
PspGI	CCWGG	4 cut(s)	144, 634, 1398, 1546
PspN4I	GGNNCC	4 cut(s)	162, 837, 1437, 1459
PspPI	GGNCC	2 cut(s)	385, 1684
PstI	CTGCAG	1 cut(s)	1585
PstNI	CAGNNNCTG	1 cut(s)	977
PsuI	RGATCY	1 cut(s)	51
PsyI	GACNNNGTC	1 cut(s)	1682
PvuII	CAGCTG	1 cut(s)	1586
RigI	GGCCGGCC	1 cut(s)	391
RsaI	GTAC	7 cut(s)	15, 88, 254, 1042, 1273, 1341, 1459
RsaNI	GTAC	7 cut(s)	14, 87, 253, 1041, 1272, 1340, 1458
RseI	CAYNNNNRTG	3 cut(s)	1179, 1430, 1670
SacI	GAGCTC	1 cut(s)	274
SalI	GTCGAC	1 cut(s)	751
SaqAI	TTAA	3 cut(s)	330, 1515, 1527
SatI	GCNGC	7 cut(s)	177, 309, 398, 616, 963, 1581, 1584
Sau3AI	GATC	5 cut(s)	23, 51, 904, 1489, 1743
Sau96I	GGNCC	2 cut(s)	385, 1684
ScaI	AGTACT	1 cut(s)	1042
SchI	GAGTC	5 cut(s)	748, 983, 1018, 1116, 1647
ScrFI	CCNGG	5 cut(s)	43, 146, 636, 1400, 1548
SduI	GDGCHC	2 cut(s)	274, 1634
SfaNI	GCATC	5 cut(s)	598, 807, 1402, 1470, 1608
SfcI	CTRYAG	1 cut(s)	1581
SfiI	GGCCNNNNNGGCC	1 cut(s)	308
SfoI	GGCGCC	1 cut(s)	162
SfuI	TTCGAA	1 cut(s)	624
SinI	GGWCC	1 cut(s)	1684
SmiMI	CAYNNNNRTG	3 cut(s)	1179, 1430, 1670
SmlI	CTYRAG	1 cut(s)	1196
SmoI	CTYRAG	1 cut(s)	1196
SphI	GCATGC	1 cut(s)	1429
Sse9I	AATT	9 cut(s)	349, 434, 466, 565, 710, 1014, 1691, 1709, 1772
SseBI	AGGCCT	1 cut(s)	302
SsiI	CCGC	6 cut(s)	177, 209, 308, 616, 963, 1789
SspDI	GGCGCC	1 cut(s)	160
SspMI	CTAG	3 cut(s)	1499, 1523, 1659
SstI	GAGCTC	1 cut(s)	274
StuI	AGGCCT	1 cut(s)	302
StyD4I	CCNGG	5 cut(s)	41, 144, 634, 1398, 1546
StyI	CCWWGG	3 cut(s)	392, 839, 1159
TaaI	ACNGT	2 cut(s)	556, 1699
TaiI	ACGT	2 cut(s)	105, 219
TaqI	TCGA	8 cut(s)	22, 318, 624, 752, 1052, 1210, 1668, 1746
TasI	AATT	9 cut(s)	349, 434, 466, 565, 710, 1014, 1691, 1709, 1772
TatI	WGTACW	4 cut(s)	86, 252, 1040, 1271
TauI	GCSGC	4 cut(s)	179, 311, 618, 965
TfiI	GAWTC	2 cut(s)	415, 644
Tru1I	TTAA	3 cut(s)	330, 1515, 1527
Tru9I	TTAA	3 cut(s)	330, 1515, 1527
TseFI	GTSAC	1 cut(s)	1589
TseI	GCWGC	3 cut(s)	397, 1580, 1583
Tsp45I	GTSAC	1 cut(s)	1589
TspDTI	ATGAA	7 cut(s)	407, 710, 714, 995, 1110, 1240, 1704
TspGWI	ACGGA	1 cut(s)	64
Tth111I	GACNNNGTC	1 cut(s)	1682
VpaK11BI	GGWCC	1 cut(s)	1684
VspI	ATTAAT	1 cut(s)	330
XagI	CCTNNNNNAGG	1 cut(s)	143
XapI	RAATTY	3 cut(s)	434, 565, 1691
XceI	RCATGY	3 cut(s)	1072, 1429, 1476
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1649
XmiI	GTMKAC	1 cut(s)	752
XmnI	GAANNNNTTC	2 cut(s)	1299, 1555
XspI	CTAG	3 cut(s)	1499, 1523, 1659
ZrmI	AGTACT	1 cut(s)	1042
Zsp2I	ATGCAT	1 cut(s)	1485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.