Rw2G049390

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016571
Physical Location & Seq
Reverse (-)
77017937 .. 77019502
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049390.1

Sequence Viewer

Length: 1482 bp
ATGGCCCTCGCCGTCAACGTCTTCAACCAAGTCCACCACCCCAATGTTCACTTGTACAACACTCTCATAAGAGCTCACATTCACAACTCCCAGAGCTCCCAGGCTTTCGCGGCCTTCTTCGATATGCAGGTGAATGGGGTTTACCCTGATAATTTCACCTTCCCTTTTCTTCTAAAGGCGTGTTCCGGCAGGAATTGGCTGCCTGTAGTGCAGATGATTCATGCTCAGGTTGAGAAATTCGGGTTCTGTGGCGATATTTTCGTGCCGAATTCGCTGATTGATACTTACTCGAAGTGCGGATTGTTTGGTGTTAGTGAGGCGAGGAAGCTGTTTAGGGTGATGGGTGAGAGAGATATTGTTTCTTGGAACTCTATGATTGGGGGGCTGGCGAAAGCTGGAGAGTTGAGTGATGCACGCAACTTGTTCGATGATATGCCTGAGAGAGATGCGGTGAGCTGGAATTCGATACTGGATGGGATGCCACAGAGGAATGTGGTGTCTTGGTCGACTTTGGTTTCGGGTTATAGCAAAGTTGGGGATATGGGGATGGCGAGGATGATGTTTGATAGGATGCCTTTTAAGAATTTGGTTCCGTGGACCATTATTATATCTGGGTATGCTCAGAAGGGGCTTGCCAAGGAGGCGATTATGTTGTATGATCAGATGGAGAGAGATGGATTGAAGCTGGATAATGGGGCTATTATTAGTATATTGGCTGCTTGCGCGGAGTCTGGTTTGATTGCATTGGGGAAGAAAGTTCATGAATCTATTGAGAGGAATAGATTCAAGTGTAGTACTCCGGTTTTGAATGCGTTGCTTGATATGTATGCAAAGTGTGCAAAGAAAGATATGGTGTCTTGGAATTCAATGCTCCAAGGGCTAGCCATGCATGGACATGGTGAGAAAGCACTTCAGCTGTTCTCTAGAATGGAGAAAGCTGGGTTTAGGCCGGATAAGGTCACCTTTATCGGTGTGTTATGTGCCTGCACGCATGCAGGCTTTGTGGAGGAGGGGCTTCATGCATTCCATACTATGGAAAAGGAGTATGGGATTGTACCTGAAATTGAGCATTACGGTTGCATGATTGACCTTTTGGGTCGAGGAGGGCGTCTTAGGGAAGCTTTTAGGCTTGTGCATAGCATGCCAATGGAACCGAATGCTGTTATATGGGGTACCCTTTTAGGGGCGTGCCGGATGCATAATGACCTTGAACTAGCAGAGGAGGCACTGGATCAGCTTGTTAAGTTAGAGCCATCAGATCCAGGAAATTTTTCTATCGTCTCAAATATGTATGCTGCAGCTGGTGATTGGGAAAACATGGCCAATATGAGGCTACAAATGAGGAGCATAGGAGTCCAGAAAACTTCAGGGGCTAGTTCCATCGAGCTGGATGATGAGGTACATGAATTTACAGTATTCGATAGAGTTCATCCCAAATCGAATGACATATATGGGATGATTAACAGACTAGGTGAGACCTGA

Protein Analysis

493

Amino Acids

55.01

Weight (kDa)

6.02

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 14 - 61 2.9e-10 PPR repeat family
PPR_2 PF13041 117 - 158 1.7e-07 PPR repeat family
PPR_1 PF12854 117 - 145 2.7e-06 PPR repeat
PPR PF01535 120 - 148 2.1e-07 PPR repeat
PPR_2 PF13041 195 - 241 7e-07 PPR repeat family
PPR PF01535 198 - 227 4.5e-07 PPR repeat
PPR_2 PF13041 283 - 329 7.4e-12 PPR repeat family
PPR PF01535 285 - 315 7.2e-08 PPR repeat
E_motif PF20431 400 - 462 1.3e-16 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016571)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29230
fragaria_vesca FvH4_6g47760
malus_domestica MD17G1054700.v1.1
prunus_persica Prupe.3G261400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0166911
rosa_laevigata RLG00000021678
rosa_multiflora Rmu_sc0002735.1_g000016 Rmu_sc0005344.1_g000009
rosa_roxburghii Rroxscaffold_2G00084020
rosa_rugosa Rorug02G0526300
rosa_samantha Rh2BG604800 Rh2CG575500 Rh2DG616400
rosa_wichuraiana Rw2G049390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 118
Acc36I ACCTGC 1 cut(s) 118
Acc65I GGTACC 1 cut(s) 1172
AccB1I GGYRCC 1 cut(s) 1172
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccI GTMKAC 1 cut(s) 506
AccII CGCG 2 cut(s) 110, 725
AciI CCGC 4 cut(s) 110, 297, 449, 725
AclWI GGATC 2 cut(s) 1239, 1253
AcoI YGGCCR 1 cut(s) 1320
AcsI RAATTY 7 cut(s) 236, 268, 460, 583, 862, 1267, 1406
AcuI CTGAAG 2 cut(s) 896, 1350
AcyI GRCGYC 1 cut(s) 1108
AfaI GTAC 5 cut(s) 56, 796, 1056, 1174, 1401
AfiI CCNNNNNNNGG 4 cut(s) 1033, 1182, 1183, 1329
AgsI TTSAA 6 cut(s) 25, 682, 787, 808, 867, 1211
AjnI CCWGG 2 cut(s) 99, 1261
Alw21I GWGCWC 2 cut(s) 76, 98
Alw26I GTCTC 2 cut(s) 1285, 1469
AlwI GGATC 2 cut(s) 1239, 1253
AoxI GGCC 4 cut(s) 3, 111, 947, 1320
ApeKI GCWGC 4 cut(s) 199, 716, 1295, 1298
ApoI RAATTY 7 cut(s) 236, 268, 460, 583, 862, 1267, 1406
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
Asp700I GAANNNNTTC 2 cut(s) 782, 1270
Asp718I GGTACC 1 cut(s) 1172
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 2 cut(s) 4, 597
AsuHPI GGTGA 8 cut(s) 142, 148, 349, 356, 463, 911, 952, 1316
AsuNHI GCTAGC 1 cut(s) 880
AvaII GGWCC 1 cut(s) 597
BalI TGGCCA 1 cut(s) 1322
BanI GGYRCC 1 cut(s) 1172
BanII GRGCYC 2 cut(s) 76, 98
BbsI GAAGAC 1 cut(s) 13
Bbv12I GWGCWC 2 cut(s) 76, 98
BbvI GCAGC 4 cut(s) 186, 703, 1282, 1310
BccI CCATC 7 cut(s) 334, 467, 541, 658, 668, 1261, 1388
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 2 cut(s) 101, 1263
BclI TGATCA 1 cut(s) 658
BcoDI GTCTC 2 cut(s) 1285, 1469
BfaI CTAG 5 cut(s) 881, 924, 1214, 1374, 1469
BfmI CTRYAG 2 cut(s) 204, 1296
BfuAI ACCTGC 1 cut(s) 118
BglI GCCNNNNNGGC 1 cut(s) 641
BisI GCNGC 5 cut(s) 111, 200, 717, 1296, 1299
BlsI GCNGC 5 cut(s) 112, 201, 718, 1297, 1300
BmcAI AGTACT 1 cut(s) 796
Bme1390I CCNGG 2 cut(s) 101, 1263
Bme18I GGWCC 1 cut(s) 597
BmgT120I GGNCC 2 cut(s) 4, 597
BmiI GGNNCC 3 cut(s) 591, 1152, 1174
BmrFI CCNGG 2 cut(s) 101, 1263
BmsI GCATC 5 cut(s) 400, 436, 468, 561, 1185
BmtI GCTAGC 1 cut(s) 884
BpiI GAAGAC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 417
Bpu10I CCTNAGC 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 1108
BsaI GGTCTC 1 cut(s) 1469
BsaJI CCNNGG 4 cut(s) 99, 593, 636, 874
BsaWI WCCGGW 1 cut(s) 799
Bsc4I CCNNNNNNNGG 4 cut(s) 1033, 1182, 1183, 1329
Bse1I ACTGG 2 cut(s) 474, 1233
BseBI CCWGG 2 cut(s) 101, 1263
BseDI CCNNGG 4 cut(s) 99, 593, 636, 874
BseGI GGATG 9 cut(s) 478, 483, 552, 561, 576, 1200, 1396, 1429, 1461
BseLI CCNNNNNNNGG 4 cut(s) 1033, 1182, 1183, 1329
BseMII CTCAG 3 cut(s) 239, 429, 635
BseNI ACTGG 2 cut(s) 474, 1233
BseRI GAGGAG 4 cut(s) 1022, 1116, 1235, 1357
BseXI GCAGC 4 cut(s) 186, 703, 1282, 1310
BseYI CCCAGC 1 cut(s) 938
BsgI GTGCAG 2 cut(s) 230, 970
Bsh1236I CGCG 2 cut(s) 110, 725
BshFI GGCC 4 cut(s) 5, 113, 949, 1322
BshNI GGYRCC 1 cut(s) 1172
BsiHKAI GWGCWC 2 cut(s) 76, 98
BsiSI CCGG 4 cut(s) 186, 800, 950, 1192
BslI CCNNNNNNNGG 4 cut(s) 1033, 1182, 1183, 1329
BsmAI GTCTC 2 cut(s) 1285, 1469
BsmBI CGTCTC 1 cut(s) 1285
BsmI GAATGC 3 cut(s) 814, 1022, 1162
BsnI GGCC 4 cut(s) 5, 113, 949, 1322
Bso31I GGTCTC 1 cut(s) 1469
Bsp1286I GDGCHC 2 cut(s) 76, 98
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 3 cut(s) 658, 1231, 1258
BspACI CCGC 4 cut(s) 110, 297, 449, 725
BspANI GGCC 4 cut(s) 5, 113, 949, 1322
BspCNI CTCAG 3 cut(s) 238, 430, 634
BspFNI CGCG 2 cut(s) 110, 725
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 3 cut(s) 591, 1152, 1174
BspMAI CTGCAG 1 cut(s) 1300
BspMI ACCTGC 1 cut(s) 118
BspOI GCTAGC 1 cut(s) 884
BspPI GGATC 2 cut(s) 1239, 1253
BspT107I GGYRCC 1 cut(s) 1172
BspTNI GGTCTC 1 cut(s) 1469
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 2 cut(s) 474, 1233
BssECI CCNNGG 4 cut(s) 99, 593, 636, 874
BssMI GATC 3 cut(s) 658, 1231, 1258
BssNI GRCGYC 1 cut(s) 1108
BssT1I CCWWGG 2 cut(s) 636, 874
Bst2UI CCWGG 2 cut(s) 101, 1263
Bst4CI ACNGT 2 cut(s) 1076, 1414
BstACI GRCGYC 1 cut(s) 1108
BstAPI GCANNNNNTGC 2 cut(s) 836, 1141
BstAUI TGTACA 1 cut(s) 54
BstDEI CTNAG 4 cut(s) 225, 438, 621, 1112
BstDSI CCRYGG 1 cut(s) 593
BstEII GGTNACC 1 cut(s) 958
BstF5I GGATG 9 cut(s) 478, 483, 552, 561, 576, 1200, 1396, 1429, 1461
BstFNI CGCG 2 cut(s) 110, 725
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 3 cut(s) 661, 1234, 1261
BstMAI GTCTC 2 cut(s) 1285, 1469
BstMBI GATC 3 cut(s) 658, 1231, 1258
BstNI CCWGG 2 cut(s) 101, 1263
BstNSI RCATGY 2 cut(s) 995, 1144
BstPI GGTNACC 1 cut(s) 958
BstSCI CCNGG 2 cut(s) 99, 1261
BstSFI CTRYAG 2 cut(s) 204, 1296
BstUI CGCG 2 cut(s) 110, 725
BstV1I GCAGC 4 cut(s) 186, 703, 1282, 1310
BstV2I GAAGAC 1 cut(s) 13
BstX2I RGATCY 1 cut(s) 1258
BstXI CCANNNNNNTGG 1 cut(s) 1387
BstYI RGATCY 1 cut(s) 1258
BsuRI GGCC 4 cut(s) 5, 113, 949, 1322
BtgI CCRYGG 1 cut(s) 593
BtsCI GGATG 9 cut(s) 478, 483, 552, 561, 576, 1200, 1396, 1429, 1461
BtsIMutI CAGTG 1 cut(s) 1226
BveI ACCTGC 1 cut(s) 118
CciI TCATGA 1 cut(s) 760
CfoI GCGC 1 cut(s) 725
Cfr13I GGNCC 2 cut(s) 4, 597
CseI GACGC 1 cut(s) 1097
Csp6I GTAC 5 cut(s) 55, 795, 1055, 1173, 1400
CviQI GTAC 5 cut(s) 55, 795, 1055, 1173, 1400
DdeI CTNAG 4 cut(s) 225, 438, 621, 1112
DpnI GATC 3 cut(s) 660, 1233, 1260
DpnII GATC 3 cut(s) 658, 1231, 1258
EaeI YGGCCR 1 cut(s) 1320
Ecl136II GAGCTC 2 cut(s) 74, 96
Eco130I CCWWGG 2 cut(s) 636, 874
Eco24I GRGCYC 2 cut(s) 76, 98
Eco31I GGTCTC 1 cut(s) 1469
Eco47I GGWCC 1 cut(s) 597
Eco53kI GAGCTC 2 cut(s) 74, 96
Eco57I CTGAAG 2 cut(s) 896, 1350
Eco91I GGTNACC 1 cut(s) 958
EcoICRI GAGCTC 2 cut(s) 74, 96
EcoO65I GGTNACC 1 cut(s) 958
EcoRI GAATTC 3 cut(s) 268, 460, 862
EcoRII CCWGG 2 cut(s) 99, 1261
EcoT14I CCWWGG 2 cut(s) 636, 874
EcoT22I ATGCAT 3 cut(s) 891, 1024, 1200
EcoT38I GRGCYC 2 cut(s) 76, 98
ErhI CCWWGG 2 cut(s) 636, 874
Esp3I CGTCTC 1 cut(s) 1285
FalI AAGNNNNNCTT 2 cut(s) 947, 979
FbaI TGATCA 1 cut(s) 658
FblI GTMKAC 1 cut(s) 506
Fnu4HI GCNGC 5 cut(s) 111, 200, 717, 1296, 1299
FokI GGATG 9 cut(s) 485, 490, 559, 568, 583, 1207, 1403, 1416, 1468
FriOI GRGCYC 2 cut(s) 76, 98
Fsp4HI GCNGC 5 cut(s) 111, 200, 717, 1296, 1299
FspBI CTAG 5 cut(s) 881, 924, 1214, 1374, 1469
GlaI GCGC 1 cut(s) 724
GluI GCNGC 5 cut(s) 111, 200, 717, 1296, 1299
GsaI CCCAGC 1 cut(s) 942
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 4 cut(s) 5, 113, 949, 1322
HapII CCGG 4 cut(s) 186, 800, 950, 1192
HgaI GACGC 1 cut(s) 1097
HhaI GCGC 1 cut(s) 725
Hin1I GRCGYC 1 cut(s) 1108
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HincII GTYRAC 2 cut(s) 16, 507
HindII GTYRAC 2 cut(s) 16, 507
HindIII AAGCTT 1 cut(s) 1119
HinfI GANTC 5 cut(s) 217, 728, 764, 783, 1353
HpaII CCGG 4 cut(s) 186, 800, 950, 1192
HphI GGTGA 8 cut(s) 142, 148, 349, 356, 463, 911, 952, 1316
Hpy166II GTNNAC 6 cut(s) 16, 34, 49, 142, 507, 597
Hpy188I TCNGA 3 cut(s) 624, 663, 1258
Hpy188III TCNNGA 3 cut(s) 761, 924, 1357
Hpy8I GTNNAC 6 cut(s) 16, 34, 49, 142, 507, 597
HpyAV CCTTC 3 cut(s) 124, 169, 619
HpyCH4III ACNGT 2 cut(s) 1076, 1414
HpyCH4IV ACGT 1 cut(s) 18
HpyF3I CTNAG 4 cut(s) 225, 438, 621, 1112
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 1108
HspAI GCGC 1 cut(s) 723
KpnI GGTACC 1 cut(s) 1176
Ksp22I TGATCA 1 cut(s) 658
Kzo9I GATC 3 cut(s) 658, 1231, 1258
LmnI GCTCC 3 cut(s) 101, 876, 1344
Lsp1109I GCAGC 4 cut(s) 186, 703, 1282, 1310
LweI GCATC 5 cut(s) 400, 436, 468, 561, 1185
MaeI CTAG 5 cut(s) 881, 924, 1214, 1374, 1469
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 958
MalI GATC 3 cut(s) 660, 1233, 1260
MboI GATC 3 cut(s) 658, 1231, 1258
MboII GAAGA 4 cut(s) 13, 109, 161, 763
MflI RGATCY 1 cut(s) 1258
MhlI GDGCHC 2 cut(s) 76, 98
MlsI TGGCCA 1 cut(s) 1322
MluNI TGGCCA 1 cut(s) 1322
MlyI GAGTC 2 cut(s) 737, 1362
Mox20I TGGCCA 1 cut(s) 1322
Mph1103I ATGCAT 3 cut(s) 891, 1024, 1200
MroXI GAANNNNTTC 2 cut(s) 782, 1270
MscI TGGCCA 1 cut(s) 1322
MseI TTAA 3 cut(s) 579, 1242, 1461
MslI CAYNNNNRTG 3 cut(s) 42, 894, 1145
Msp20I TGGCCA 1 cut(s) 1322
MspA1I CMGCKG 2 cut(s) 916, 1301
MspI CCGG 4 cut(s) 186, 800, 950, 1192
MspR9I CCNGG 2 cut(s) 101, 1263
Mva1269I GAATGC 3 cut(s) 814, 1022, 1162
MvaI CCWGG 2 cut(s) 101, 1263
MvnI CGCG 2 cut(s) 110, 725
NdeII GATC 3 cut(s) 658, 1231, 1258
NheI GCTAGC 1 cut(s) 880
NlaIV GGNNCC 3 cut(s) 591, 1152, 1174
NmuCI GTSAC 1 cut(s) 958
NsiI ATGCAT 3 cut(s) 891, 1024, 1200
NspI RCATGY 2 cut(s) 995, 1144
PaeI GCATGC 2 cut(s) 995, 1144
PagI TCATGA 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 118
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 1105
PctI GAATGC 3 cut(s) 814, 1022, 1162
PdmI GAANNNNTTC 2 cut(s) 782, 1270
PfeI GAWTC 3 cut(s) 217, 764, 783
PflMI CCANNNNNTGG 1 cut(s) 1033
PfoI TCCNGGA 1 cut(s) 1261
PkrI GCNGC 5 cut(s) 112, 201, 718, 1297, 1300
PleI GAGTC 2 cut(s) 736, 1361
PpsI GAGTC 2 cut(s) 736, 1361
Psp124BI GAGCTC 2 cut(s) 76, 98
Psp6I CCWGG 2 cut(s) 99, 1261
PspEI GGTNACC 1 cut(s) 958
PspFI CCCAGC 1 cut(s) 938
PspGI CCWGG 2 cut(s) 99, 1261
PspN4I GGNNCC 3 cut(s) 591, 1152, 1174
PspPI GGNCC 2 cut(s) 4, 597
PstI CTGCAG 1 cut(s) 1300
PsuI RGATCY 1 cut(s) 1258
PvuII CAGCTG 2 cut(s) 916, 1301
RsaI GTAC 5 cut(s) 56, 796, 1056, 1174, 1401
RsaNI GTAC 5 cut(s) 55, 795, 1055, 1173, 1400
RseI CAYNNNNRTG 3 cut(s) 42, 894, 1145
SacI GAGCTC 2 cut(s) 76, 98
SalI GTCGAC 1 cut(s) 505
SaqAI TTAA 3 cut(s) 579, 1242, 1461
SatI GCNGC 5 cut(s) 111, 200, 717, 1296, 1299
Sau3AI GATC 3 cut(s) 658, 1231, 1258
Sau96I GGNCC 2 cut(s) 4, 597
ScaI AGTACT 1 cut(s) 796
SchI GAGTC 2 cut(s) 737, 1362
ScrFI CCNGG 2 cut(s) 101, 1263
SduI GDGCHC 2 cut(s) 76, 98
SfaNI GCATC 5 cut(s) 400, 436, 468, 561, 1185
SfcI CTRYAG 2 cut(s) 204, 1296
SinI GGWCC 1 cut(s) 597
SmiMI CAYNNNNRTG 3 cut(s) 42, 894, 1145
SphI GCATGC 2 cut(s) 995, 1144
SsiI CCGC 4 cut(s) 110, 297, 449, 725
SspMI CTAG 5 cut(s) 881, 924, 1214, 1374, 1469
SstI GAGCTC 2 cut(s) 76, 98
StyD4I CCNGG 2 cut(s) 99, 1261
StyI CCWWGG 2 cut(s) 636, 874
TaaI ACNGT 2 cut(s) 1076, 1414
TaiI ACGT 1 cut(s) 21
TaqI TCGA 9 cut(s) 120, 290, 426, 464, 506, 1099, 1383, 1419, 1439
TatI WGTACW 2 cut(s) 54, 794
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 3 cut(s) 217, 764, 783
Tru1I TTAA 3 cut(s) 579, 1242, 1461
Tru9I TTAA 3 cut(s) 579, 1242, 1461
TscAI CASTG 1 cut(s) 1233
TseFI GTSAC 1 cut(s) 958
TseI GCWGC 4 cut(s) 199, 716, 1295, 1298
Tsp45I GTSAC 1 cut(s) 958
TspDTI ATGAA 6 cut(s) 209, 749, 777, 1007, 1418, 1419
TspGWI ACGGA 1 cut(s) 582
TspRI CASTG 1 cut(s) 1233
Van91I CCANNNNNTGG 1 cut(s) 1033
VpaK11BI GGWCC 1 cut(s) 597
XapI RAATTY 7 cut(s) 236, 268, 460, 583, 862, 1267, 1406
XbaI TCTAGA 1 cut(s) 923
XceI RCATGY 2 cut(s) 995, 1144
XmiI GTMKAC 1 cut(s) 506
XmnI GAANNNNTTC 2 cut(s) 782, 1270
XspI CTAG 5 cut(s) 881, 924, 1214, 1374, 1469
ZrmI AGTACT 1 cut(s) 796
Zsp2I ATGCAT 3 cut(s) 891, 1024, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.