MD17G1097400.v1.1

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0024313
Physical Location & Seq
Forward (+)
8241203 .. 8241643
441 bp
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UTR
Exon/CDS
Intron
MD17G1097400.v1.1.491

Sequence Viewer

Length: 441 bp
ATGCAAGTGTGGTTTCAGACCATGCTAAACGTCGGCACACCTAGCCAGCTCGCTTCGATGCTGCCTGCAGAATGTACGAGCCGGTTATTTCCTGCAGGAATTAGTGATGATCGGGAAAGTCAGAAACAGATGAAGCAGTTCTTGGTCATGATGGATTCGATGACAGCTCAAGAGTTGGATAGCCCGAACCTCAAGATAAGCGAGTTGCGGATAAAACGGATAGCACAAGGTTCTGGTCATCAAGTTAGACAAGTAGAAGAGCTGATTAAAGCGTGCAAAGCTGCTAGCAAGAATTTTAGCAAGAGGAAAGCGCTGCTTCGGAGTTTGAACAGCGGCGACATGCGTGAGTTAACCCGGAATATGAGTAGTAATCCGCAGCTGCTCAACCAGATTGGGGGCATGGGTGGTTTGAGGAGCTTGATGAATCTGAGGAGGCTGTAA

Protein Analysis

147

Amino Acids

16.4

Weight (kDa)

10.28

Isoelectric Point (pI)

63.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP_SPB PF02978 5 - 97 4.6e-21 Signal peptide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024313)

Species Orthologous Gene IDs
malus_domestica MD09G1109100.v1.1 MD17G1097400.v1.1
pyrus_communis pycom09g03160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 208, 333, 374
AcsI RAATTY 1 cut(s) 292
AfaI GTAC 1 cut(s) 76
AfeI AGCGCT 1 cut(s) 312
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 125, 157
AluBI AGCT 6 cut(s) 49, 167, 262, 281, 379, 417
AluI AGCT 6 cut(s) 49, 167, 262, 281, 379, 417
Aor51HI AGCGCT 1 cut(s) 312
ApeKI GCWGC 5 cut(s) 61, 281, 313, 376, 379
ApoI RAATTY 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 137
AspLEI GCGC 1 cut(s) 313
AsuC2I CCSGG 1 cut(s) 355
AsuNHI GCTAGC 1 cut(s) 284
BbvI GCAGC 5 cut(s) 48, 268, 300, 366, 388
BccI CCATC 1 cut(s) 145
BcnI CCSGG 1 cut(s) 355
BfaI CTAG 2 cut(s) 42, 285
BfmI CTRYAG 2 cut(s) 66, 93
BfoI RGCGCY 1 cut(s) 314
BisI GCNGC 6 cut(s) 62, 282, 314, 334, 377, 380
BlsI GCNGC 6 cut(s) 63, 283, 315, 335, 378, 381
Bme1390I CCNGG 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 355
BmsI GCATC 1 cut(s) 48
BmtI GCTAGC 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 153, 176
BpuMI CCSGG 1 cut(s) 355
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse118I RCCGGY 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMII CTCAG 1 cut(s) 419
BseRI GAGGAG 1 cut(s) 427
BseXI GCAGC 5 cut(s) 48, 268, 300, 366, 388
BsiSI CCGG 2 cut(s) 82, 355
BslI CCNNNNNNNGG 1 cut(s) 394
Bsp143I GATC 1 cut(s) 109
BspACI CCGC 3 cut(s) 208, 333, 374
BspCNI CTCAG 1 cut(s) 420
BspHI TCATGA 1 cut(s) 147
BspMAI CTGCAG 2 cut(s) 70, 97
BspOI GCTAGC 1 cut(s) 288
BspQI GCTCTTC 1 cut(s) 252
BsrFI RCCGGY 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 81
BssMI GATC 1 cut(s) 109
Bst6I CTCTTC 1 cut(s) 252
BstC8I GCNNGC 5 cut(s) 47, 51, 66, 274, 286
BstDEI CTNAG 1 cut(s) 428
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 1 cut(s) 112
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 2 cut(s) 42, 278
BstNSI RCATGY 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 353
BstSFI CTRYAG 2 cut(s) 66, 93
BstV1I GCAGC 5 cut(s) 48, 268, 300, 366, 388
Cac8I GCNNGC 5 cut(s) 47, 51, 66, 274, 286
CciI TCATGA 1 cut(s) 147
CfoI GCGC 1 cut(s) 313
Cfr10I RCCGGY 1 cut(s) 81
Csp6I GTAC 1 cut(s) 75
CviAII CATG 4 cut(s) 22, 148, 340, 400
CviQI GTAC 1 cut(s) 75
DdeI CTNAG 1 cut(s) 428
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco47III AGCGCT 1 cut(s) 312
FaeI CATG 4 cut(s) 25, 151, 343, 403
FaiI YATR 5 cut(s) 23, 149, 341, 362, 401
FalI AAGNNNNNCTT 4 cut(s) 125, 157, 300, 332
FatI CATG 4 cut(s) 21, 147, 339, 399
Fnu4HI GCNGC 6 cut(s) 62, 282, 314, 334, 377, 380
Fsp4HI GCNGC 6 cut(s) 62, 282, 314, 334, 377, 380
FspBI CTAG 2 cut(s) 42, 285
GlaI GCGC 1 cut(s) 312
GluI GCNGC 6 cut(s) 62, 282, 314, 334, 377, 380
HaeII RGCGCY 1 cut(s) 314
HapII CCGG 2 cut(s) 82, 355
HhaI GCGC 1 cut(s) 313
Hin1II CATG 4 cut(s) 25, 151, 343, 403
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HinfI GANTC 2 cut(s) 155, 424
HpaI GTTAAC 1 cut(s) 351
HpaII CCGG 2 cut(s) 82, 355
Hpy166II GTNNAC 1 cut(s) 351
Hpy188I TCNGA 4 cut(s) 18, 123, 321, 429
Hpy188III TCNNGA 4 cut(s) 113, 148, 170, 193
Hpy8I GTNNAC 1 cut(s) 351
Hpy99I CGWCG 1 cut(s) 35
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 4 cut(s) 4, 68, 95, 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 278
HpyF3I CTNAG 1 cut(s) 428
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 4 cut(s) 25, 151, 343, 403
HspAI GCGC 1 cut(s) 311
KspAI GTTAAC 1 cut(s) 351
Kzo9I GATC 1 cut(s) 109
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 1 cut(s) 414
LpnPI CCDG 8 cut(s) 59, 78, 81, 95, 105, 219, 368, 401
Lsp1109I GCAGC 5 cut(s) 48, 268, 300, 366, 388
LweI GCATC 1 cut(s) 48
MaeI CTAG 2 cut(s) 42, 285
MaeII ACGT 1 cut(s) 30
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MboII GAAGA 1 cut(s) 269
MluCI AATT 2 cut(s) 99, 292
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 5 cut(s) 200, 297, 405, 423, 426
MroXI GAANNNNTTC 1 cut(s) 137
MseI TTAA 2 cut(s) 267, 350
MspA1I CMGCKG 2 cut(s) 333, 379
MspI CCGG 2 cut(s) 82, 355
MspR9I CCNGG 1 cut(s) 355
MwoI GCNNNNNNNGC 2 cut(s) 42, 278
NciI CCSGG 1 cut(s) 355
NdeII GATC 1 cut(s) 109
NheI GCTAGC 1 cut(s) 284
NlaIII CATG 4 cut(s) 25, 151, 343, 403
NspI RCATGY 1 cut(s) 343
PagI TCATGA 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 252
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 2 cut(s) 155, 424
PkrI GCNGC 6 cut(s) 63, 283, 315, 335, 378, 381
PstI CTGCAG 2 cut(s) 70, 97
PvuII CAGCTG 1 cut(s) 379
RsaI GTAC 1 cut(s) 76
RsaNI GTAC 1 cut(s) 75
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 2 cut(s) 267, 350
SatI GCNGC 6 cut(s) 62, 282, 314, 334, 377, 380
Sau3AI GATC 1 cut(s) 109
SbfI CCTGCAGG 1 cut(s) 97
ScrFI CCNGG 1 cut(s) 355
SdaI CCTGCAGG 1 cut(s) 97
SfaNI GCATC 1 cut(s) 48
SfcI CTRYAG 2 cut(s) 66, 93
SmlI CTYRAG 2 cut(s) 168, 191
SmoI CTYRAG 2 cut(s) 168, 191
Sse8387I CCTGCAGG 1 cut(s) 97
Sse9I AATT 2 cut(s) 99, 292
SsiI CCGC 3 cut(s) 208, 333, 374
SspMI CTAG 2 cut(s) 42, 285
StyD4I CCNGG 1 cut(s) 353
TaiI ACGT 1 cut(s) 33
TaqI TCGA 2 cut(s) 56, 158
TasI AATT 2 cut(s) 99, 292
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 2 cut(s) 155, 424
Tru1I TTAA 2 cut(s) 267, 350
Tru9I TTAA 2 cut(s) 267, 350
TseI GCWGC 5 cut(s) 61, 281, 313, 376, 379
TspDTI ATGAA 2 cut(s) 146, 437
TspGWI ACGGA 1 cut(s) 232
XapI RAATTY 1 cut(s) 292
XceI RCATGY 1 cut(s) 343
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 2 cut(s) 42, 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.