MD17G1142300.v1.1

Serine threonine-protein phosphatase 7 long form homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004585
Physical Location & Seq
Forward (+)
12823885 .. 12825817
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1142300.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGGAAATGATACTGGCGGATCCTTATGGGACGAATCCTGGACCAATTGATGGCTCAGTGCTTTATGATCAGGACAAGCATGTGTCTTCAGCAGTTTGGGATGGACAGGAGCGTGGTGCACTCAGATGCCACGAACACACTTCAAAGCTTGATCAATGGACACTCACGGAAAAACAGGTTGAGTTGGTAGAGAAGGCTGGATTTGGTTATTTAAGATGGATTCCGGCAATTAGTCTTGATAACCCACTCATTTCTGCACTAGTTGAAAGGTGGAGGAGAGAAACAAACACTTTTCACCTTAATGATGGAGAAATGACGGTAACTCTTCAGGATGTCGCACTATTGCTTGGACTAGCAATTGATGGAGAACCTGTAATTGGTACAACACATACCACCTGCAACTCAGTTTGTGACAGGTATCTCGGAAAAGTACCAGAGCCTAGTTATACTAGCGGTGGAATGGTGAAGCTAAGCTGGTTGAAAGAGTTCTTTTCCCATTGTCCCAAAGATGCACCACTTGAAGTGATTGAATGTCATACCCGTGCGTACCTCTTATACCTTGTTGGAAGTACAATATTTTCCACAACTACTGGGAATAAAGTCCCTGTCATGTACCTTCCACTGTTTGAGAATTTTGACGATTGTGGGAGATATGCCTGGGGGGCAGCAGCATTGGCATTCTTGTACAGGGCACTGGGCAATGCGTCCCTGAGATCTCAAAGTACAATTAGTGGCTGTTTAACGCTACTACAGTGCTGGAGTTACTTTCACCTAAATATTGGACGACCAAAGCTCAATACTGATTCAATGCATGATCGTTTTCCTTTTGTGCTTAGCTGGAAGGGAAAGCAAAGTGGGCCAACAACAAACCGTGATGTAGTGTTTTACCGTAAAGCTCTGGATTCCTTGAAACCATGTGATGCGGAGTGGCTTCCGTATAGAAATATGGACAGTACAGTGATTCCAGAAGATATCAAGAGCAATCTGATTATAGGGAGGTCCAAAACAATGCTAATATGCTTTGACAAGGCGGAAAGGCATCTCCCAAATCGTTGCTTAAGGCAATACGGCAAGTTTCAGTCAATCCCAGAGGATGTACAGCGATGGGAGAGAAAAAGTCGTGGAGTTGATGGTGGGGTTGACTTGTCAGGGAAAATGGAGTCAGAGCTTAATGAATGGCTAGAACGTCAATATCATATTGTGGATGGTGATGATGGTGCAGATGAAAGTGATTATATGCTGTGGTACTTGAAAATTACACGCAAATTTATAGGGAGGCCTATATCTCTCTCGTCTGAGTTTCAAAGAACGGTAAGATTAATGCTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

444

Amino Acids

50.38

Weight (kDa)

6.04

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 67 - 416 1.5e-110 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 404
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 4 cut(s) 17, 453, 923, 1031
AclWI GGATC 2 cut(s) 14, 27
AcsI RAATTY 2 cut(s) 631, 1265
AcuI CTGAAG 2 cut(s) 72, 311
AfiI CCNNNNNNNGG 2 cut(s) 50, 377
AflII CTTAAG 1 cut(s) 1057
AgsI TTSAA 9 cut(s) 144, 266, 481, 521, 530, 807, 910, 1252, 1304
AhlI ACTAGT 1 cut(s) 259
AjnI CCWGG 2 cut(s) 37, 656
AjuI GAANNNNNNNTTGG 2 cut(s) 360, 392
AluBI AGCT 7 cut(s) 148, 469, 474, 793, 837, 896, 1168
AluI AGCT 7 cut(s) 148, 469, 474, 793, 837, 896, 1168
Alw21I GWGCWC 1 cut(s) 121
Alw44I GTGCAC 1 cut(s) 117
AlwI GGATC 2 cut(s) 14, 27
AoxI GGCC 2 cut(s) 857, 1277
ApaLI GTGCAC 1 cut(s) 117
ApeKI GCWGC 2 cut(s) 665, 668
ApoI RAATTY 2 cut(s) 631, 1265
AseI ATTAAT 1 cut(s) 1319
Asp700I GAANNNNTTC 1 cut(s) 485
AspS9I GGNCC 3 cut(s) 41, 857, 999
AsuHPI GGTGA 4 cut(s) 287, 475, 761, 1220
AvaII GGWCC 2 cut(s) 41, 999
BaeGI GKGCMC 2 cut(s) 121, 694
BamHI GGATCC 1 cut(s) 19
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 677, 680
BccI CCATC 9 cut(s) 44, 95, 210, 299, 356, 1098, 1124, 1199, 1208
BceAI ACGGC 1 cut(s) 1084
BciT130I CCWGG 2 cut(s) 39, 658
BclI TGATCA 2 cut(s) 67, 151
BcuI ACTAGT 1 cut(s) 259
BfaI CTAG 5 cut(s) 260, 353, 441, 450, 1181
BfmI CTRYAG 1 cut(s) 749
BfrI CTTAAG 1 cut(s) 1057
BfuAI ACCTGC 1 cut(s) 404
BglI GCCNNNNNGGC 1 cut(s) 662
BglII AGATCT 1 cut(s) 713
BisI GCNGC 2 cut(s) 666, 669
BlpI GCTNAGC 2 cut(s) 470, 833
BlsI GCNGC 2 cut(s) 667, 670
Bme1390I CCNGG 2 cut(s) 39, 658
Bme18I GGWCC 2 cut(s) 41, 999
BmgT120I GGNCC 3 cut(s) 41, 857, 999
BmiI GGNNCC 1 cut(s) 21
BmrFI CCNGG 2 cut(s) 39, 658
BmrI ACTGGG 2 cut(s) 600, 704
BmsI GCATC 4 cut(s) 116, 499, 910, 1048
BmuI ACTGGG 2 cut(s) 600, 704
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 778
Bpu1102I GCTNAGC 2 cut(s) 470, 833
BsaJI CCNNGG 1 cut(s) 657
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 377
Bse1I ACTGG 3 cut(s) 18, 595, 699
Bse3DI GCAATG 1 cut(s) 706
BseBI CCWGG 2 cut(s) 39, 658
BseDI CCNNGG 1 cut(s) 657
BseGI GGATG 4 cut(s) 106, 337, 1099, 1210
BseLI CCNNNNNNNGG 2 cut(s) 50, 377
BseMI GCAATG 1 cut(s) 706
BseMII CTCAG 5 cut(s) 69, 136, 417, 701, 1287
BseNI ACTGG 3 cut(s) 18, 595, 699
BseRI GAGGAG 1 cut(s) 289
BseSI GKGCMC 2 cut(s) 121, 694
BseXI GCAGC 2 cut(s) 677, 680
BseYI CCCAGC 1 cut(s) 1324
BsgI GTGCAG 2 cut(s) 240, 1239
BshFI GGCC 2 cut(s) 859, 1279
BsiHKAI GWGCWC 1 cut(s) 121
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 4 cut(s) 43, 486, 587, 691
BslI CCNNNNNNNGG 2 cut(s) 50, 377
BsmFI GGGAC 4 cut(s) 43, 486, 587, 691
BsmI GAATGC 1 cut(s) 677
BsnI GGCC 2 cut(s) 859, 1279
Bsp1286I GDGCHC 2 cut(s) 121, 694
Bsp1407I TGTACA 2 cut(s) 684, 1096
Bsp143I GATC 5 cut(s) 19, 67, 151, 713, 814
Bsp1720I GCTNAGC 2 cut(s) 470, 833
BspACI CCGC 4 cut(s) 17, 453, 923, 1031
BspANI GGCC 2 cut(s) 859, 1279
BspCNI CTCAG 5 cut(s) 68, 135, 416, 702, 1288
BspLI GGNNCC 1 cut(s) 21
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 2 cut(s) 14, 27
BspTI CTTAAG 1 cut(s) 1057
BsrDI GCAATG 1 cut(s) 706
BsrGI TGTACA 2 cut(s) 684, 1096
BsrI ACTGG 3 cut(s) 18, 595, 699
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 5 cut(s) 19, 67, 151, 713, 814
Bst2UI CCWGG 2 cut(s) 39, 658
Bst4CI ACNGT 8 cut(s) 319, 624, 753, 872, 890, 953, 958, 1312
Bst6I CTCTTC 1 cut(s) 330
BstAFI CTTAAG 1 cut(s) 1057
BstAUI TGTACA 2 cut(s) 684, 1096
BstDEI CTNAG 7 cut(s) 55, 122, 403, 470, 710, 833, 1296
BstF5I GGATG 4 cut(s) 106, 337, 1099, 1210
BstKTI GATC 5 cut(s) 22, 70, 154, 716, 817
BstMBI GATC 5 cut(s) 19, 67, 151, 713, 814
BstMWI GCNNNNNNNGC 3 cut(s) 662, 674, 856
BstNI CCWGG 2 cut(s) 39, 658
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 2 cut(s) 37, 656
BstSFI CTRYAG 1 cut(s) 749
BstSLI GKGCMC 2 cut(s) 121, 694
BstV1I GCAGC 2 cut(s) 677, 680
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 19, 713
BstYI RGATCY 2 cut(s) 19, 713
BsuRI GGCC 2 cut(s) 859, 1279
BtgZI GCGATG 1 cut(s) 1117
BtsCI GGATG 4 cut(s) 106, 337, 1099, 1210
BtsIMutI CAGTG 5 cut(s) 63, 620, 692, 758, 963
BveI ACCTGC 1 cut(s) 404
Cfr13I GGNCC 3 cut(s) 41, 857, 999
CseI GACGC 1 cut(s) 693
CviAII CATG 4 cut(s) 80, 610, 812, 915
DdeI CTNAG 7 cut(s) 55, 122, 403, 470, 710, 833, 1296
DpnI GATC 5 cut(s) 21, 69, 153, 715, 816
DpnII GATC 5 cut(s) 19, 67, 151, 713, 814
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 2 cut(s) 32, 1046
Eco147I AGGCCT 1 cut(s) 1279
Eco32I GATATC 1 cut(s) 973
Eco47I GGWCC 2 cut(s) 41, 999
Eco57I CTGAAG 2 cut(s) 72, 311
EcoRII CCWGG 2 cut(s) 37, 656
EcoRV GATATC 1 cut(s) 973
EcoT22I ATGCAT 1 cut(s) 813
FaeI CATG 4 cut(s) 83, 613, 815, 918
FaqI GGGAC 4 cut(s) 43, 486, 587, 691
FatI CATG 4 cut(s) 79, 609, 811, 914
FbaI TGATCA 2 cut(s) 67, 151
Fnu4HI GCNGC 2 cut(s) 666, 669
FokI GGATG 4 cut(s) 113, 344, 1106, 1217
Fsp4HI GCNGC 2 cut(s) 666, 669
FspBI CTAG 5 cut(s) 260, 353, 441, 450, 1181
GluI GCNGC 2 cut(s) 666, 669
GsaI CCCAGC 1 cut(s) 1328
GsuI CTGGAG 1 cut(s) 778
HaeIII GGCC 2 cut(s) 859, 1279
HapII CCGG 1 cut(s) 224
HgaI GACGC 1 cut(s) 693
Hin1II CATG 4 cut(s) 83, 613, 815, 918
HincII GTYRAC 1 cut(s) 1141
HindII GTYRAC 1 cut(s) 1141
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 6 cut(s) 34, 220, 803, 902, 961, 1160
HpaII CCGG 1 cut(s) 224
HphI GGTGA 4 cut(s) 287, 475, 761, 1220
Hpy166II GTNNAC 2 cut(s) 119, 1141
Hpy188I TCNGA 5 cut(s) 125, 425, 987, 1165, 1297
Hpy188III TCNNGA 6 cut(s) 71, 236, 329, 899, 965, 976
Hpy8I GTNNAC 2 cut(s) 119, 1141
HpyAV CCTTC 3 cut(s) 187, 626, 835
HpyCH4III ACNGT 8 cut(s) 319, 624, 753, 872, 890, 953, 958, 1312
HpyCH4IV ACGT 1 cut(s) 1186
HpyCH4V TGCA 6 cut(s) 119, 257, 399, 512, 811, 1220
HpyF10VI GCNNNNNNNGC 3 cut(s) 662, 674, 856
HpyF3I CTNAG 7 cut(s) 55, 122, 403, 470, 710, 833, 1296
HpySE526I ACGT 1 cut(s) 1186
Hsp92II CATG 4 cut(s) 83, 613, 815, 918
Ksp22I TGATCA 2 cut(s) 67, 151
Kzo9I GATC 5 cut(s) 19, 67, 151, 713, 814
LmnI GCTCC 1 cut(s) 109
Lsp1109I GCAGC 2 cut(s) 677, 680
LweI GCATC 4 cut(s) 116, 499, 910, 1048
MaeI CTAG 5 cut(s) 260, 353, 441, 450, 1181
MaeII ACGT 1 cut(s) 1186
MaeIII GTNAC 3 cut(s) 319, 410, 761
MalI GATC 5 cut(s) 21, 69, 153, 715, 816
MboI GATC 5 cut(s) 19, 67, 151, 713, 814
MboII GAAGA 3 cut(s) 78, 317, 980
MfeI CAATTG 2 cut(s) 45, 357
MflI RGATCY 2 cut(s) 19, 713
MhlI GDGCHC 2 cut(s) 121, 694
MluCI AATT 8 cut(s) 45, 228, 357, 375, 631, 726, 1254, 1265
MlyI GAGTC 1 cut(s) 1169
MmeI TCCRAC 1 cut(s) 544
MnlI CCTC 5 cut(s) 267, 560, 990, 1084, 1269
Mph1103I ATGCAT 1 cut(s) 813
MroXI GAANNNNTTC 1 cut(s) 485
MseI TTAA 6 cut(s) 212, 300, 740, 1058, 1170, 1319
MslI CAYNNNNRTG 3 cut(s) 124, 300, 540
MspCI CTTAAG 1 cut(s) 1057
MspI CCGG 1 cut(s) 224
MspR9I CCNGG 2 cut(s) 39, 658
MunI CAATTG 2 cut(s) 45, 357
Mva1269I GAATGC 1 cut(s) 677
MvaI CCWGG 2 cut(s) 39, 658
MwoI GCNNNNNNNGC 3 cut(s) 662, 674, 856
NdeII GATC 5 cut(s) 19, 67, 151, 713, 814
NlaIII CATG 4 cut(s) 83, 613, 815, 918
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 1 cut(s) 410
NsiI ATGCAT 1 cut(s) 813
NspI RCATGY 1 cut(s) 83
PaqCI CACCTGC 1 cut(s) 404
PceI AGGCCT 1 cut(s) 1279
PctI GAATGC 1 cut(s) 677
PdmI GAANNNNTTC 1 cut(s) 485
PfeI GAWTC 5 cut(s) 34, 220, 803, 902, 961
PflMI CCANNNNNTGG 1 cut(s) 50
PfoI TCCNGGA 1 cut(s) 37
PkrI GCNGC 2 cut(s) 667, 670
PleI GAGTC 1 cut(s) 1168
PpsI GAGTC 1 cut(s) 1168
PshBI ATTAAT 1 cut(s) 1319
Psp6I CCWGG 2 cut(s) 37, 656
PspFI CCCAGC 1 cut(s) 1324
PspGI CCWGG 2 cut(s) 37, 656
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 3 cut(s) 41, 857, 999
PsuI RGATCY 2 cut(s) 19, 713
RseI CAYNNNNRTG 3 cut(s) 124, 300, 540
SaqAI TTAA 6 cut(s) 212, 300, 740, 1058, 1170, 1319
SatI GCNGC 2 cut(s) 666, 669
Sau3AI GATC 5 cut(s) 19, 67, 151, 713, 814
Sau96I GGNCC 3 cut(s) 41, 857, 999
SchI GAGTC 1 cut(s) 1169
ScrFI CCNGG 2 cut(s) 39, 658
SduI GDGCHC 2 cut(s) 121, 694
SfaNI GCATC 4 cut(s) 116, 499, 910, 1048
SfcI CTRYAG 1 cut(s) 749
SinI GGWCC 2 cut(s) 41, 999
SmiMI CAYNNNNRTG 3 cut(s) 124, 300, 540
SmlI CTYRAG 1 cut(s) 1057
SmoI CTYRAG 1 cut(s) 1057
SpeI ACTAGT 1 cut(s) 259
Sse9I AATT 8 cut(s) 45, 228, 357, 375, 631, 726, 1254, 1265
SseBI AGGCCT 1 cut(s) 1279
SsiI CCGC 4 cut(s) 17, 453, 923, 1031
SspI AATATT 2 cut(s) 576, 778
SspMI CTAG 5 cut(s) 260, 353, 441, 450, 1181
StuI AGGCCT 1 cut(s) 1279
StyD4I CCNGG 2 cut(s) 37, 656
TaaI ACNGT 8 cut(s) 319, 624, 753, 872, 890, 953, 958, 1312
TaiI ACGT 1 cut(s) 1189
TasI AATT 8 cut(s) 45, 228, 357, 375, 631, 726, 1254, 1265
TatI WGTACW 5 cut(s) 569, 684, 722, 953, 1096
TfiI GAWTC 5 cut(s) 34, 220, 803, 902, 961
Tru1I TTAA 6 cut(s) 212, 300, 740, 1058, 1170, 1319
Tru9I TTAA 6 cut(s) 212, 300, 740, 1058, 1170, 1319
TscAI CASTG 5 cut(s) 63, 627, 699, 758, 963
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 2 cut(s) 665, 668
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 2 cut(s) 1188, 1239
TspGWI ACGGA 2 cut(s) 182, 924
TspRI CASTG 5 cut(s) 63, 627, 699, 758, 963
Van91I CCANNNNNTGG 1 cut(s) 50
Vha464I CTTAAG 1 cut(s) 1057
VneI GTGCAC 1 cut(s) 117
VpaK11BI GGWCC 2 cut(s) 41, 999
VspI ATTAAT 1 cut(s) 1319
XapI RAATTY 2 cut(s) 631, 1265
XceI RCATGY 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 485
XspI CTAG 5 cut(s) 260, 353, 441, 450, 1181
Zsp2I ATGCAT 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.