MD17G1255600.v1.1

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0024447
Physical Location & Seq
Reverse (-)
31238888 .. 31241027
2140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1255600.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGGGATTACAGGGATGATATATATGGTTACAATGGTATTTTCATTAATTGTATTAATATTGTCTTCGTCCGCGGTGAAATTCGATTATTTTCAATTTACGCAGCAATATCAGCCGGCTGTCTGCAGCTCTAATCCTACTCCTTGTAAGGATCCTCCTGACAAGTTGTTTACGGTTCATGGTTTGTGGCCTTCAAATGTTAATGGAAGTGACCCCAAGAAATGCAAAACTACAATCTTGAACCCTCAAACGATAACAAATCTTACAGCCCAGCTGGAAATTATTTGGCCAAACGTACTCAATCGAAAGGCTCATGCACGCTTCTGGCGTAAACAGTGGCGTAAACATGGCACCTGTGGGTACCCCACAATAGCGGACGACATGCATTACTTTAGCACAGTAATCGAAATGTACATAACCAAGAAACAAAATGTCTCTGAAATCCTCTCAAAGGCGAAAATTAAACCGGAGAAGAAATTCAGGACACGGGACGACATTGTGAATGCCATAAGCCAAAGTATCGACTATAAGAAACCAAAACTCAAGTGCAAGAACAATAATCAGATAACTGAATTGGTTGAGGTCGGTCTTTGCAGCGATAACAACTTAACGCAGTTCATAGATTGCCCCCGCCCATTTCCACAAGGATCACCATTTTTCTGCCCCACCAATAATATTCAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

229

Amino Acids

26.14

Weight (kDa)

9.29

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 209 2.6e-39 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024447)

Species Orthologous Gene IDs
malus_domestica MD17G1200900.v1.1 MD17G1255600.v1.1
pyrus_communis pycom17g25810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 360
AccB1I GGYRCC 2 cut(s) 350, 360
AccII CGCG 1 cut(s) 74
AciI CCGC 4 cut(s) 72, 74, 374, 631
AclWI GGATC 3 cut(s) 146, 159, 655
AcoI YGGCCR 1 cut(s) 287
AcsI RAATTY 2 cut(s) 80, 476
AfaI GTAC 3 cut(s) 297, 362, 413
AfiI CCNNNNNNNGG 1 cut(s) 451
AgsI TTSAA 3 cut(s) 95, 195, 241
AluBI AGCT 2 cut(s) 129, 274
AluI AGCT 2 cut(s) 129, 274
Alw26I GTCTC 1 cut(s) 439
AlwI GGATC 3 cut(s) 146, 159, 655
AoxI GGCC 2 cut(s) 188, 287
ApeKI GCWGC 3 cut(s) 103, 126, 594
ApoI RAATTY 2 cut(s) 80, 476
AseI ATTAAT 2 cut(s) 47, 56
Asp700I GAANNNNTTC 1 cut(s) 476
Asp718I GGTACC 1 cut(s) 360
AsuHPI GGTGA 2 cut(s) 88, 642
BalI TGGCCA 1 cut(s) 289
BamHI GGATCC 1 cut(s) 151
BanI GGYRCC 2 cut(s) 350, 360
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 3 cut(s) 115, 138, 606
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BcoDI GTCTC 1 cut(s) 439
BfmI CTRYAG 1 cut(s) 124
BisI GCNGC 3 cut(s) 104, 127, 595
BlsI GCNGC 3 cut(s) 105, 128, 596
BmiI GGNNCC 3 cut(s) 153, 352, 362
BpiI GAAGAC 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 527
BsaJI CCNNGG 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 466
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse118I RCCGGY 1 cut(s) 115
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 451
BseXI GCAGC 3 cut(s) 115, 138, 606
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 2 cut(s) 190, 289
BshNI GGYRCC 2 cut(s) 350, 360
BsiSI CCGG 2 cut(s) 116, 467
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 1 cut(s) 451
BsmAI GTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 503
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 2 cut(s) 190, 289
Bsp1407I TGTACA 1 cut(s) 411
Bsp143I GATC 2 cut(s) 151, 647
BspACI CCGC 4 cut(s) 72, 74, 374, 631
BspANI GGCC 2 cut(s) 190, 289
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 3 cut(s) 153, 352, 362
BspMAI CTGCAG 1 cut(s) 128
BspPI GGATC 3 cut(s) 146, 159, 655
BspT107I GGYRCC 2 cut(s) 350, 360
BsrFI RCCGGY 1 cut(s) 115
BsrGI TGTACA 1 cut(s) 411
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 2 cut(s) 151, 647
Bst4CI ACNGT 3 cut(s) 175, 336, 400
BstAUI TGTACA 1 cut(s) 411
BstC8I GCNNGC 2 cut(s) 117, 319
BstDSI CCRYGG 1 cut(s) 72
BstENI CCTNNNNNAGG 1 cut(s) 449
BstF5I GGATG 1 cut(s) 21
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 2 cut(s) 154, 650
BstMAI GTCTC 1 cut(s) 439
BstMBI GATC 2 cut(s) 151, 647
BstMWI GCNNNNNNNGC 1 cut(s) 112
BstNSI RCATGY 1 cut(s) 385
BstSFI CTRYAG 1 cut(s) 124
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 3 cut(s) 115, 138, 606
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 2 cut(s) 190, 289
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 341
Cac8I GCNNGC 2 cut(s) 117, 319
Cfr10I RCCGGY 1 cut(s) 115
Cfr42I CCGCGG 1 cut(s) 75
Csp6I GTAC 3 cut(s) 296, 361, 412
CviAII CATG 4 cut(s) 179, 314, 347, 382
CviJI RGCY 9 cut(s) 115, 119, 129, 190, 269, 274, 289, 311, 513
CviKI_1 RGCY 9 cut(s) 115, 119, 129, 190, 269, 274, 289, 311, 513
CviQI GTAC 3 cut(s) 296, 361, 412
DpnI GATC 2 cut(s) 153, 649
DpnII GATC 2 cut(s) 151, 647
EaeI YGGCCR 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 449
EcoT22I ATGCAT 1 cut(s) 387
FaeI CATG 4 cut(s) 182, 317, 350, 385
FaqI GGGAC 1 cut(s) 503
FatI CATG 4 cut(s) 178, 313, 346, 381
FauI CCCGC 1 cut(s) 638
Fnu4HI GCNGC 3 cut(s) 104, 127, 595
FokI GGATG 1 cut(s) 28
Fsp4HI GCNGC 3 cut(s) 104, 127, 595
GluI GCNGC 3 cut(s) 104, 127, 595
GsaI CCCAGC 1 cut(s) 274
HaeIII GGCC 2 cut(s) 190, 289
HapII CCGG 2 cut(s) 116, 467
Hin1II CATG 4 cut(s) 182, 317, 350, 385
HpaII CCGG 2 cut(s) 116, 467
HphI GGTGA 2 cut(s) 88, 642
Hpy166II GTNNAC 3 cut(s) 171, 332, 344
Hpy188I TCNGA 2 cut(s) 439, 564
Hpy188III TCNNGA 3 cut(s) 158, 238, 481
Hpy8I GTNNAC 3 cut(s) 171, 332, 344
HpyAV CCTTC 1 cut(s) 201
HpyCH4III ACNGT 3 cut(s) 175, 336, 400
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 6 cut(s) 126, 225, 317, 385, 549, 594
HpyF10VI GCNNNNNNNGC 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 4 cut(s) 182, 317, 350, 385
KpnI GGTACC 1 cut(s) 364
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
KspI CCGCGG 1 cut(s) 75
Kzo9I GATC 2 cut(s) 151, 647
LpnPI CCDG 8 cut(s) 129, 171, 260, 284, 310, 367, 466, 480
Lsp1109I GCAGC 3 cut(s) 115, 138, 606
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 2 cut(s) 28, 209
MalI GATC 2 cut(s) 153, 649
MboI GATC 2 cut(s) 151, 647
MboII GAAGA 2 cut(s) 57, 484
MflI RGATCY 1 cut(s) 151
MlsI TGGCCA 1 cut(s) 289
MluCI AATT 7 cut(s) 48, 80, 95, 279, 459, 476, 572
MluNI TGGCCA 1 cut(s) 289
MnlI CCTC 4 cut(s) 165, 255, 455, 574
Mox20I TGGCCA 1 cut(s) 289
Mph1103I ATGCAT 1 cut(s) 387
MroNI GCCGGC 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 476
MscI TGGCCA 1 cut(s) 289
MseI TTAA 6 cut(s) 47, 56, 201, 462, 608, 685
Msp20I TGGCCA 1 cut(s) 289
MspA1I CMGCKG 2 cut(s) 74, 274
MspI CCGG 2 cut(s) 116, 467
Mva1269I GAATGC 1 cut(s) 508
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 112
NaeI GCCGGC 1 cut(s) 117
NdeII GATC 2 cut(s) 151, 647
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 4 cut(s) 182, 317, 350, 385
NlaIV GGNNCC 3 cut(s) 153, 352, 362
NmuCI GTSAC 1 cut(s) 209
NsiI ATGCAT 1 cut(s) 387
NspI RCATGY 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 325
PctI GAATGC 1 cut(s) 508
PdiI GCCGGC 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 476
PkrI GCNGC 3 cut(s) 105, 128, 596
PshBI ATTAAT 2 cut(s) 47, 56
PspFI CCCAGC 1 cut(s) 270
PspN4I GGNNCC 3 cut(s) 153, 352, 362
PstI CTGCAG 1 cut(s) 128
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 274
RsaI GTAC 3 cut(s) 297, 362, 413
RsaNI GTAC 3 cut(s) 296, 361, 412
SacII CCGCGG 1 cut(s) 75
SaqAI TTAA 6 cut(s) 47, 56, 201, 462, 608, 685
SatI GCNGC 3 cut(s) 104, 127, 595
Sau3AI GATC 2 cut(s) 151, 647
SetI ASST 5 cut(s) 131, 276, 297, 356, 585
SfcI CTRYAG 1 cut(s) 124
Sfr303I CCGCGG 1 cut(s) 75
SgrBI CCGCGG 1 cut(s) 75
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 7 cut(s) 48, 80, 95, 279, 459, 476, 572
SsiI CCGC 4 cut(s) 72, 74, 374, 631
SspI AATATT 2 cut(s) 60, 676
TaaI ACNGT 3 cut(s) 175, 336, 400
TaiI ACGT 1 cut(s) 297
TaqI TCGA 4 cut(s) 84, 304, 405, 522
TaqII GACCGA 1 cut(s) 575
TasI AATT 7 cut(s) 48, 80, 95, 279, 459, 476, 572
TatI WGTACW 1 cut(s) 411
Tru1I TTAA 6 cut(s) 47, 56, 201, 462, 608, 685
Tru9I TTAA 6 cut(s) 47, 56, 201, 462, 608, 685
TscAI CASTG 1 cut(s) 341
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 3 cut(s) 103, 126, 594
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 3 cut(s) 33, 167, 607
TspRI CASTG 1 cut(s) 341
VspI ATTAAT 2 cut(s) 47, 56
XagI CCTNNNNNAGG 1 cut(s) 449
XapI RAATTY 2 cut(s) 80, 476
XceI RCATGY 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 476
Zsp2I ATGCAT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.