pycom17g25810

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0024447
Physical Location & Seq
Reverse (-)
24014113 .. 24015597
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g25810.1

Sequence Viewer

Length: 699 bp
ATGGGGATTACGAGGATGATATATATGGTTACGATGGCATTTTCATTAATTGTATTAATATTGTCTTCGTCCACGATGGGGTACGATTATTTTCAATTTACGCAGCAATATCAGCCGGCTGCCTGCAATTCTAATCCTACTCCTTGTAAGGATCCTACTGAGAAATTGTTTACGGTTCACGGTTTGTGGCCTTCAAACTCTAATGGACCTGACCCAGTAAACTGCAAGCCGAAAACCAAGGTGCCTCAGGCGCAGCAGCCGATAGATCCATCACTCAAACCCCAGTTGGAAATTATTTGGCCGAACGTATTCAATCGAGCCGATAATGAAAGCTTCTGGAATAAACAGTGGGACAAACATGGCACCTGTGGGTATCCCACAATAAAAGACAAGAACCATTACCTTCAAACAGTAATCAAAATGTACATAACCCAGAAACAAAACGTCTCTCAAATCCTATCCAAAGCCAACATCAATCCAGACGGTATAGGCAGGACACGGAAGCTAATTGAAAATGCCATACGCAATGGTACCAACGATAAGGAACCAAAACTCAAGTGCCAAAAGAATAATGGGACTATTGAATTGGTCGAGGTCAGTCTTTGCAGCAATTACCTCGGAAAACATTTCATAAATTGCCCCAACAAAATTCCACAAGGATCACGATATTTCTGCCCCATCAAAGATATCCAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

233

Amino Acids

26.46

Weight (kDa)

9.19

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 213 4.2e-38 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024447)

Species Orthologous Gene IDs
malus_domestica MD17G1200900.v1.1 MD17G1255600.v1.1
pyrus_communis pycom17g25810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 530
AccB1I GGYRCC 3 cut(s) 241, 362, 530
AclWI GGATC 4 cut(s) 146, 159, 260, 667
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 1 cut(s) 648
AfaI GTAC 3 cut(s) 83, 425, 532
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 6 cut(s) 95, 195, 313, 407, 512, 584
AluBI AGCT 2 cut(s) 333, 505
AluI AGCT 2 cut(s) 333, 505
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 4 cut(s) 146, 159, 260, 667
AoxI GGCC 2 cut(s) 188, 299
ApeKI GCWGC 5 cut(s) 103, 119, 253, 256, 606
ApoI RAATTY 1 cut(s) 648
AseI ATTAAT 2 cut(s) 47, 56
Asp700I GAANNNNTTC 1 cut(s) 308
Asp718I GGTACC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 1 cut(s) 206
AvaII GGWCC 1 cut(s) 206
AxyI CCTNAGG 1 cut(s) 246
BamHI GGATCC 1 cut(s) 151
BanI GGYRCC 3 cut(s) 241, 362, 530
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 5 cut(s) 106, 115, 265, 268, 618
BccI CCATC 4 cut(s) 28, 70, 277, 686
BcgI CGANNNNNNTGC 4 cut(s) 598, 632, 654, 688
BciVI GTATCC 1 cut(s) 384
BcoDI GTCTC 1 cut(s) 451
BfuI GTATCC 1 cut(s) 384
BisI GCNGC 5 cut(s) 104, 120, 254, 257, 607
BlsI GCNGC 5 cut(s) 105, 121, 255, 258, 608
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 5 cut(s) 153, 243, 364, 532, 546
BmrI ACTGGG 2 cut(s) 209, 277
BmuI ACTGGG 2 cut(s) 209, 277
BpiI GAAGAC 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 237, 616
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 3 cut(s) 215, 283, 691
Bse21I CCTNAGG 1 cut(s) 246
Bse3DI GCAATG 1 cut(s) 532
BseDI CCNNGG 2 cut(s) 237, 616
BseGI GGATG 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 2 cut(s) 150, 260
BseNI ACTGG 3 cut(s) 215, 283, 691
BseXI GCAGC 5 cut(s) 106, 115, 265, 268, 618
BshFI GGCC 2 cut(s) 190, 301
BshNI GGYRCC 3 cut(s) 241, 362, 530
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 2 cut(s) 365, 589
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 1 cut(s) 451
BsmBI CGTCTC 1 cut(s) 451
BsmFI GGGAC 2 cut(s) 365, 589
BsnI GGCC 2 cut(s) 190, 301
Bsp1407I TGTACA 1 cut(s) 423
Bsp143I GATC 3 cut(s) 151, 265, 659
BspANI GGCC 2 cut(s) 190, 301
BspCNI CTCAG 2 cut(s) 151, 259
BspLI GGNNCC 5 cut(s) 153, 243, 364, 532, 546
BspPI GGATC 4 cut(s) 146, 159, 260, 667
BspT107I GGYRCC 3 cut(s) 241, 362, 530
BsrDI GCAATG 1 cut(s) 532
BsrFI RCCGGY 1 cut(s) 115
BsrGI TGTACA 1 cut(s) 423
BsrI ACTGG 3 cut(s) 215, 283, 691
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 2 cut(s) 237, 616
BssMI GATC 3 cut(s) 151, 265, 659
BssT1I CCWWGG 1 cut(s) 237
Bst4CI ACNGT 5 cut(s) 175, 182, 348, 412, 485
BstAUI TGTACA 1 cut(s) 423
BstC8I GCNNGC 3 cut(s) 117, 124, 227
BstDEI CTNAG 2 cut(s) 159, 246
BstF5I GGATG 1 cut(s) 21
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 3 cut(s) 154, 268, 662
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 3 cut(s) 151, 265, 659
BstMWI GCNNNNNNNGC 2 cut(s) 112, 250
BstV1I GCAGC 5 cut(s) 106, 115, 265, 268, 618
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 2 cut(s) 151, 265
BstYI RGATCY 2 cut(s) 151, 265
Bsu36I CCTNAGG 1 cut(s) 246
BsuI GTATCC 1 cut(s) 384
BsuRI GGCC 2 cut(s) 190, 301
BtsCI GGATG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 3 cut(s) 117, 124, 227
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 3 cut(s) 82, 424, 531
CviAII CATG 1 cut(s) 359
CviQI GTAC 3 cut(s) 82, 424, 531
DdeI CTNAG 2 cut(s) 159, 246
DpnI GATC 3 cut(s) 153, 267, 661
DpnII GATC 3 cut(s) 151, 265, 659
EaeI YGGCCR 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 237
Eco32I GATATC 1 cut(s) 688
Eco47I GGWCC 1 cut(s) 206
Eco81I CCTNAGG 1 cut(s) 246
EcoRV GATATC 1 cut(s) 688
EcoT14I CCWWGG 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 237
Esp3I CGTCTC 1 cut(s) 451
FaeI CATG 1 cut(s) 362
FaiI YATR 8 cut(s) 22, 24, 26, 360, 428, 488, 521, 632
FaqI GGGAC 2 cut(s) 365, 589
FatI CATG 1 cut(s) 358
Fnu4HI GCNGC 5 cut(s) 104, 120, 254, 257, 607
FokI GGATG 1 cut(s) 28
Fsp4HI GCNGC 5 cut(s) 104, 120, 254, 257, 607
GlaI GCGC 1 cut(s) 252
GluI GCNGC 5 cut(s) 104, 120, 254, 257, 607
HaeIII GGCC 2 cut(s) 190, 301
HapII CCGG 1 cut(s) 116
HhaI GCGC 1 cut(s) 253
Hin1II CATG 1 cut(s) 362
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HindIII AAGCTT 1 cut(s) 331
HpaII CCGG 1 cut(s) 116
Hpy166II GTNNAC 4 cut(s) 72, 171, 178, 220
Hpy188I TCNGA 1 cut(s) 620
Hpy188III TCNNGA 3 cut(s) 337, 479, 663
Hpy8I GTNNAC 4 cut(s) 72, 171, 178, 220
HpyAV CCTTC 2 cut(s) 201, 413
HpyCH4III ACNGT 5 cut(s) 175, 182, 348, 412, 485
HpyCH4IV ACGT 2 cut(s) 306, 444
HpyCH4V TGCA 3 cut(s) 126, 225, 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 250
HpyF3I CTNAG 2 cut(s) 159, 246
HpySE526I ACGT 2 cut(s) 306, 444
Hsp92II CATG 1 cut(s) 362
HspAI GCGC 1 cut(s) 251
KpnI GGTACC 1 cut(s) 534
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Kzo9I GATC 3 cut(s) 151, 265, 659
Lsp1109I GCAGC 5 cut(s) 106, 115, 265, 268, 618
MaeII ACGT 2 cut(s) 306, 444
MaeIII GTNAC 1 cut(s) 28
MalI GATC 3 cut(s) 153, 267, 661
MboI GATC 3 cut(s) 151, 265, 659
MboII GAAGA 1 cut(s) 57
MflI RGATCY 2 cut(s) 151, 265
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 4 cut(s) 6, 255, 586, 626
MroNI GCCGGC 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 3 cut(s) 47, 56, 697
MspI CCGG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 112, 250
NaeI GCCGGC 1 cut(s) 117
NdeII GATC 3 cut(s) 151, 265, 659
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 1 cut(s) 362
NlaIV GGNNCC 5 cut(s) 153, 243, 364, 532, 546
PdiI GCCGGC 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 308
PkrI GCNGC 5 cut(s) 105, 121, 255, 258, 608
PshBI ATTAAT 2 cut(s) 47, 56
PspN4I GGNNCC 5 cut(s) 153, 243, 364, 532, 546
PspPI GGNCC 1 cut(s) 206
PsuI RGATCY 2 cut(s) 151, 265
RsaI GTAC 3 cut(s) 83, 425, 532
RsaNI GTAC 3 cut(s) 82, 424, 531
SaqAI TTAA 3 cut(s) 47, 56, 697
SatI GCNGC 5 cut(s) 104, 120, 254, 257, 607
Sau3AI GATC 3 cut(s) 151, 265, 659
Sau96I GGNCC 1 cut(s) 206
SinI GGWCC 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
SspI AATATT 1 cut(s) 60
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 5 cut(s) 175, 182, 348, 412, 485
TaiI ACGT 2 cut(s) 309, 447
TaqI TCGA 2 cut(s) 316, 591
TatI WGTACW 1 cut(s) 423
Tru1I TTAA 3 cut(s) 47, 56, 697
Tru9I TTAA 3 cut(s) 47, 56, 697
TscAI CASTG 1 cut(s) 353
TseI GCWGC 5 cut(s) 103, 119, 253, 256, 606
TspDTI ATGAA 3 cut(s) 33, 342, 619
TspGWI ACGGA 1 cut(s) 514
TspRI CASTG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 206
VspI ATTAAT 2 cut(s) 47, 56
XapI RAATTY 1 cut(s) 648
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.