MD17G1260800.v1.1

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0022415
Physical Location & Seq
Reverse (-)
32121367 .. 32122762
1396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1260800.v1.1.491

Sequence Viewer

Length: 462 bp
ATGCTGAAGAGGAAGGTTTTGGAGAGAATTAATTTACAGAAGCATAAGAAGACCAGTACTGATGCTACACATACTGTGAAGACGAACAGGTTTTTGATCACAGTTAATGTTCTTGGAAGCGCAGGACCGATAAGGTTTGTGGTGAACGAGGATGATCTTGTAGCTCGAGTCATCGACACTGCTCTTAAATCCTACGCCCGCGAAGGCCGGCTACCTGTTCTTGGTTCAGATGTCAAGGATTTCCTTCTACACTGTGCTAATGCAGGATCTGACGCTTTAAGTCCGTGGGAAACTATAGGATCTCGCGGGGGAAGGAACTTTGTGCTGTCCAAGAAGCAGAAGCAAGTGCAGAATAATAATATTGCTGAAAGAAAGTCGGAGATAACAGCTCCAAAGGGAACGGTGAGTAGCTGGAAGGCATGGCTTAACAAATCATTCACTTTCAAGATATCATCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

17.05

Weight (kDa)

10.25

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 27 - 109 1.1e-30 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022415)

Species Orthologous Gene IDs
malus_domestica MD17G1260800.v1.1
prunus_persica Prupe.3G162600_v2.0.a1
pyrus_communis pycom09g17850 pycom17g26150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 201, 306
AciI CCGC 2 cut(s) 199, 306
AclWI GGATC 2 cut(s) 274, 307
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 221
AgsI TTSAA 1 cut(s) 445
AluBI AGCT 3 cut(s) 164, 389, 411
AluI AGCT 3 cut(s) 164, 389, 411
AlwI GGATC 2 cut(s) 274, 307
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 1 cut(s) 205
AseI ATTAAT 1 cut(s) 30
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 154, 415
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 1 cut(s) 125
BarI GAAGNNNNNNTAC 2 cut(s) 195, 227
BbsI GAAGAC 2 cut(s) 56, 86
BclI TGATCA 1 cut(s) 96
BfmI CTRYAG 1 cut(s) 294
BmcAI AGTACT 1 cut(s) 58
Bme18I GGWCC 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 125
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 2 cut(s) 56, 86
BsaJI CCNNGG 1 cut(s) 284
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse118I RCCGGY 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 284
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 1 cut(s) 221
BseNI ACTGG 1 cut(s) 54
BsgI GTGCAG 1 cut(s) 368
Bsh1236I CGCG 2 cut(s) 201, 306
BshFI GGCC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 208
BslI CCNNNNNNNGG 1 cut(s) 221
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 165
Bsp143I GATC 4 cut(s) 96, 154, 266, 299
BspACI CCGC 2 cut(s) 199, 306
BspANI GGCC 1 cut(s) 207
BspFNI CGCG 2 cut(s) 201, 306
BspPI GGATC 2 cut(s) 274, 307
BsrFI RCCGGY 1 cut(s) 207
BsrI ACTGG 1 cut(s) 54
BssAI RCCGGY 1 cut(s) 207
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 4 cut(s) 96, 154, 266, 299
Bst4CI ACNGT 4 cut(s) 76, 103, 254, 403
Bst6I CTCTTC 1 cut(s) 2
BstC8I GCNNGC 2 cut(s) 199, 209
BstDSI CCRYGG 1 cut(s) 284
BstF5I GGATG 1 cut(s) 157
BstFNI CGCG 2 cut(s) 201, 306
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 4 cut(s) 99, 157, 269, 302
BstMBI GATC 4 cut(s) 96, 154, 266, 299
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 2 cut(s) 201, 306
BstV2I GAAGAC 2 cut(s) 56, 86
BstX2I RGATCY 2 cut(s) 266, 299
BstYI RGATCY 2 cut(s) 266, 299
BsuRI GGCC 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 284
BtsCI GGATG 1 cut(s) 157
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 3 cut(s) 177, 250, 457
Cac8I GCNNGC 2 cut(s) 199, 209
CaiI CAGNNNCTG 1 cut(s) 269
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 125
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 57
CviAII CATG 1 cut(s) 420
CviJI RGCY 6 cut(s) 164, 207, 211, 389, 411, 424
CviKI_1 RGCY 6 cut(s) 164, 207, 211, 389, 411, 424
CviQI GTAC 1 cut(s) 57
DpnI GATC 4 cut(s) 98, 156, 268, 301
DpnII GATC 4 cut(s) 96, 154, 266, 299
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
Eco32I GATATC 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 165
EcoRV GATATC 1 cut(s) 450
FaeI CATG 1 cut(s) 423
FaiI YATR 4 cut(s) 45, 72, 296, 421
FatI CATG 1 cut(s) 419
FauI CCCGC 2 cut(s) 206, 299
FbaI TGATCA 1 cut(s) 96
FokI GGATG 1 cut(s) 164
GlaI GCGC 1 cut(s) 121
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 208
HgaI GACGC 1 cut(s) 281
HhaI GCGC 1 cut(s) 122
Hin1II CATG 1 cut(s) 423
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 1 cut(s) 168
HpaII CCGG 1 cut(s) 208
HphI GGTGA 2 cut(s) 154, 415
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 3 cut(s) 229, 271, 379
Hpy188III TCNNGA 1 cut(s) 445
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 5 cut(s) 7, 197, 254, 306, 409
HpyCH4III ACNGT 4 cut(s) 76, 103, 254, 403
HpyCH4V TGCA 2 cut(s) 263, 349
Hsp92II CATG 1 cut(s) 423
HspAI GCGC 1 cut(s) 120
KroI GCCGGC 1 cut(s) 207
KroNI GCCGGC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 96
Kzo9I GATC 4 cut(s) 96, 154, 266, 299
LmnI GCTCC 1 cut(s) 394
LpnPI CCDG 7 cut(s) 67, 73, 108, 221, 228, 249, 397
LweI GCATC 1 cut(s) 52
MalI GATC 4 cut(s) 98, 156, 268, 301
MboI GATC 4 cut(s) 96, 154, 266, 299
MboII GAAGA 3 cut(s) 19, 61, 91
MflI RGATCY 2 cut(s) 266, 299
MluCI AATT 2 cut(s) 27, 31
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 2 cut(s) 3, 142
MroNI GCCGGC 1 cut(s) 207
MseI TTAA 5 cut(s) 30, 105, 186, 278, 426
MspI CCGG 1 cut(s) 208
MvnI CGCG 2 cut(s) 201, 306
NaeI GCCGGC 1 cut(s) 209
NdeII GATC 4 cut(s) 96, 154, 266, 299
NgoMIV GCCGGC 1 cut(s) 207
NlaIII CATG 1 cut(s) 423
PaeR7I CTCGAG 1 cut(s) 165
PdiI GCCGGC 1 cut(s) 209
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
PshBI ATTAAT 1 cut(s) 30
PspPI GGNCC 1 cut(s) 125
PspXI VCTCGAGB 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 2 cut(s) 266, 299
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
SaqAI TTAA 5 cut(s) 30, 105, 186, 278, 426
Sau3AI GATC 4 cut(s) 96, 154, 266, 299
Sau96I GGNCC 1 cut(s) 125
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 1 cut(s) 177
SetI ASST 7 cut(s) 18, 92, 137, 166, 217, 391, 413
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 1 cut(s) 294
Sfr274I CTCGAG 1 cut(s) 165
SinI GGWCC 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 165
SmoI CTYRAG 1 cut(s) 165
Sse9I AATT 2 cut(s) 27, 31
SsiI CCGC 2 cut(s) 199, 306
SspI AATATT 1 cut(s) 361
TaaI ACNGT 4 cut(s) 76, 103, 254, 403
TaqI TCGA 2 cut(s) 166, 174
TaqII GACCGA 1 cut(s) 142
TasI AATT 2 cut(s) 27, 31
TatI WGTACW 1 cut(s) 56
Tru1I TTAA 5 cut(s) 30, 105, 186, 278, 426
Tru9I TTAA 5 cut(s) 30, 105, 186, 278, 426
TscAI CASTG 2 cut(s) 184, 257
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 2 cut(s) 184, 257
VpaK11BI GGWCC 1 cut(s) 125
VspI ATTAAT 1 cut(s) 30
XhoI CTCGAG 1 cut(s) 165
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.