Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0017177

Physical Location & Seq

Genomic Coordinates Forward (+)

32454419 .. 32456434

Gene Structure 2016 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1263700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGGGCAAGTCGTTTTTCTACTCCCTACTCAACACCCTGGACATTCCATCCCCACGCTTTCTGTCCTCCGCCACCTCCAAAGTTGTAGGAGACTCGACCATCCCCGCCGCCGCCACAGAACAATTCACAAACCTCTGCTCCAAAGGACACATCAAACAAGCATTTGAGGGGTTCAAATCTCAGATATGGTCAGACCCATCTCTCTTCTCACACCTCCTCAAAGCATGCATCCCCAGCAAGTCCCTCTCTTTAGCAAAACAGCTCCATTCTTTGATTGTCACATCTGGGTGTTCCGCAGACAAGTTCGTATCCAATCACCTCCTCAACTTGTACTCCAAAGTCGGAGACTTGGATGCCGCTTTAACCTTGTTTGGTCATCTCCCCAGGAGAAACACAATGTCTTGCAACATTTTGATCAATGGGTACGTGCAGAAGGGTGATTTGGAAAGTGCCCAGAAGGTGTTTGATGAAATGCCTGAGAGAAATGTGGCTACGTGGAATGCCATGGTCACGGGGTTGACGCAGTTTGAATTTAACGAAGAGGGGCTGAGTCTGTTTTCTGAGATGCACGAGTTCGGGTTTTTACCCGATGAGTACACTTTGGGCAGTGTTCTGAGGGGTTGTGCTGGTTTGAGAACATTGTGTGCCGGGCGTCAGGTTCACGCTTATGTGATGAAATGCGGGTTTGAGTTTAATTTGGTCGTTGGGAGCTCTTTGGCTCATATGTATATGAAGTCGGGTAGCTTGGTGGAAGGAGAAAAAGTGATTACATCGATGCCAATTCGCAGTGTGGTTGCTTGGAATACTATTATTGCGGGAAAAGCTCAAAATGGGCATCTCGAGGGAGTGTTGGATCAGTATAACTTGATGAAAATTGCTGGATTCAGGCCTGATAAGGTCACTTTTGTTAGTGTTATTAGTTCGTGTGCGGAATTGGCAACACTTGGACAGGGTCAGCAAATTCATGCCGAAGCAATTAAAGCTGGGGCTAGCACAGTTGTTGCGGTGATTAGTTCGTTAATTAGCATGTATTCGAGATGTGGGTGTCTTGATGATTCTCTGAAAGCTTTCATGGAAAGTGAATGTGGAGATGCTGTAATGTGGAGTTCTATGATTTCTGCCTATGGGTTTCATGGACGAGGAGAGAACGCCATTAAGCTGTTTGAGGAGATGAAGCAGGAAGAATTGGAGACAAATGATGTTACTTTCTTGAGTTTGCTTTATGTGTGCAGTCATTGCGGGTTGAAGGACAAAGGACTTGAGTTATTCAACTCTATGGTCGAAAAGTATGGACTTCATCCTAAATTAGAACACTATACATGTGTGGTTGACCTGCTTGGCCGATCTGGCTGTTTGGAGGAAGCCGAGGCAATGATACGATCAATGCCTGTCAAAGCAGATGCTATCATTTGGAAAACTTTGTTATCTGCATGTAAAATCCACAAGAACGCAGACATGGCAAGAAGGATTGCTGAAGAAGTTATTAGGCAAGATCCACAGGACTCAGCGTCCTATGTGCTACTCTCGAACATCCATGCTTTAGCTCGAAGGTGGCATGATGTTTCTGAGATGAGAAAAACGATGAGAGATAGAAAGGTCAAGAAGGAGCCAGGTATAAGCTGGCTGGAAATTAAAAATCAAATTCACCAGTTCTGCATCGGTGATAAATCGCACCCCCAATCTAGAGAGATTGATTTGTATCTTAAAGAGCTAACATCAGAGTTGAAGTTGCATGGTTATGTGCCTGATATCGGCTCTGTTTTGCATGATATGGATAACGAGGAGAAAGAATATAACTTGGCACACCATAGTGAGAAGTTGGCAATCGCTTTCGCCTTAATGAACACTCCTGAGGGTGCACCAGTAAGGGTGATGAAGAATTTGCGTGTTTGCACTGATTGTCATGTTGCTATTAAGTACATATCTCTAATAAAAAACCGAGAGATTATTGTACGTGATGCTAGTAGATTTCATCACTTTAAAGATGGGAATTGTTCTTGTGGAGATTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

672

Amino Acids

74.72

Weight (kDa)

6.52

Isoelectric Point (pI)

38.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	131 - 163	3.6e-08	PPR repeat family
PPR	PF01535	134 - 163	1.8e-07	PPR repeat
PPR_2	PF13041	161 - 209	6.9e-08	PPR repeat family
PPR_2	PF13041	263 - 310	1.3e-09	PPR repeat family
PPR_2	PF13041	364 - 410	1.3e-09	PPR repeat family
PPR	PF01535	366 - 394	6.6e-08	PPR repeat
E_motif	PF20431	481 - 543	5.5e-16	E motif
Eplus_motif	PF20430	548 - 575	4.2e-06	E+ motif
DYW_deaminase	PF14432	579 - 671	2.9e-43	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017177)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41080
fragaria_vesca	FvH4_6g27771
malus_domestica	MD17G1263700.v1.1
prunus_persica	Prupe.3G112800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0131051
rosa_laevigata	RLG00000019164
rosa_roxburghii	Rroxscaffold_2G00113270
rosa_rugosa	Rorug02G0294600
rosa_samantha	Rh2BG354400 Rh2CG332900 Rh2DG372400
rosa_wichuraiana	Rw2G028130 Rw2G028170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1341
AclWI	GGATC	2 cut(s)	861, 1487
AcoI	YGGCCR	1 cut(s)	1339
AcsI	RAATTY	4 cut(s)	530, 960, 1641, 1879
AcuI	CTGAAG	1 cut(s)	1494
AcyI	GRCGYC	1 cut(s)	652
AfaI	GTAC	5 cut(s)	332, 425, 596, 1919, 1953
AfiI	CCNNNNNNNGG	1 cut(s)	1751
AflIII	ACRYGT	1 cut(s)	1319
AgsI	TTSAA	5 cut(s)	175, 530, 1246, 1270, 1726
AhdI	GACNNNNNGTC	1 cut(s)	1507
AjnI	CCWGG	3 cut(s)	36, 383, 1609
AjuI	GAANNNNNNNTTGG	2 cut(s)	425, 457
AleI	CACNNNNGTG	1 cut(s)	1809
Alw21I	GWGCWC	2 cut(s)	713, 1861
Alw26I	GTCTC	3 cut(s)	84, 339, 1184
Alw44I	GTGCAC	1 cut(s)	1857
AlwI	GGATC	2 cut(s)	861, 1487
Ama87I	CYCGRG	1 cut(s)	839
AoxI	GGCC	2 cut(s)	887, 1339
ApaLI	GTGCAC	1 cut(s)	1857
ApoI	RAATTY	4 cut(s)	530, 960, 1641, 1879
Asp700I	GAANNNNTTC	1 cut(s)	1067
AsuC2I	CCSGG	1 cut(s)	649
AsuHPI	GGTGA	6 cut(s)	308, 449, 1018, 1637, 1673, 1882
AsuNHI	GCTAGC	1 cut(s)	989
AvaI	CYCGRG	1 cut(s)	839
AxyI	CCTNAGG	1 cut(s)	1851
BaeGI	GKGCMC	2 cut(s)	454, 1861
BanII	GRGCYC	1 cut(s)	713
BauI	CACGAG	1 cut(s)	569
Bbv12I	GWGCWC	2 cut(s)	713, 1861
BccI	CCATC	4 cut(s)	55, 107, 205, 1979
BciT130I	CCWGG	3 cut(s)	38, 385, 1611
BciVI	GTATCC	1 cut(s)	319
BclI	TGATCA	1 cut(s)	414
BcnI	CCSGG	1 cut(s)	649
BcoDI	GTCTC	3 cut(s)	84, 339, 1184
BfaI	CTAG	3 cut(s)	990, 1683, 1962
BfuAI	ACCTGC	1 cut(s)	1341
BfuI	GTATCC	1 cut(s)	319
BglI	GCCNNNNNGGC	1 cut(s)	1347
BisI	GCNGC	3 cut(s)	108, 111, 357
BlsI	GCNGC	3 cut(s)	109, 112, 358
Bme1390I	CCNGG	4 cut(s)	38, 385, 649, 1611
BmeRI	GACNNNNNGTC	1 cut(s)	1507
BmeT110I	CYCGRG	1 cut(s)	839
BmiI	GGNNCC	1 cut(s)	1608
BmrFI	CCNGG	4 cut(s)	38, 385, 649, 1611
BmsI	GCATC	9 cut(s)	237, 343, 555, 765, 844, 1081, 1390, 1665, 1948
BmtI	GCTAGC	1 cut(s)	993
BpuEI	CTTGAG	2 cut(s)	1231, 1280
BpuMI	CCSGG	1 cut(s)	649
Bsa29I	ATCGAT	1 cut(s)	773
BsaAI	YACGTR	3 cut(s)	427, 495, 1955
BsaBI	GATNNNNATC	1 cut(s)	1698
BsaHI	GRCGYC	1 cut(s)	652
BsaJI	CCNNGG	4 cut(s)	36, 383, 504, 1365
BsaXI	ACNNNNNCTCC	8 cut(s)	122, 152, 317, 347, 747, 777, 1080, 1110
Bsc4I	CCNNNNNNNGG	1 cut(s)	1751
Bse1I	ACTGG	3 cut(s)	1648, 1862, 2015
Bse21I	CCTNAGG	1 cut(s)	1851
Bse3DI	GCAATG	2 cut(s)	1234, 1377
Bse8I	GATNNNNATC	1 cut(s)	1698
BseBI	CCWGG	3 cut(s)	38, 385, 1611
BseCI	ATCGAT	1 cut(s)	773
BseDI	CCNNGG	4 cut(s)	36, 383, 504, 1365
BseGI	GGATG	6 cut(s)	47, 99, 228, 358, 1297, 1530
BseJI	GATNNNNATC	1 cut(s)	1698
BseLI	CCNNNNNNNGG	1 cut(s)	1751
BseMI	GCAATG	2 cut(s)	1234, 1377
BseMII	CTCAG	8 cut(s)	194, 468, 539, 552, 605, 1518, 1557, 1842
BseNI	ACTGG	3 cut(s)	1648, 1862, 2015
BseRI	GAGGAG	5 cut(s)	206, 311, 1155, 1181, 1796
BseSI	GKGCMC	2 cut(s)	454, 1861
BseYI	CCCAGC	2 cut(s)	233, 983
BsgI	GTGCAG	2 cut(s)	449, 1249
BshFI	GGCC	2 cut(s)	889, 1341
BshVI	ATCGAT	1 cut(s)	773
BsiHKAI	GWGCWC	2 cut(s)	713, 1861
BsiHKCI	CYCGRG	1 cut(s)	839
BsiSI	CCGG	1 cut(s)	648
BslFI	GGGAC	1 cut(s)	226
BslI	CCNNNNNNNGG	1 cut(s)	1751
BsmAI	GTCTC	3 cut(s)	84, 339, 1184
BsmFI	GGGAC	1 cut(s)	226
BsmI	GAATGC	1 cut(s)	505
BsnI	GGCC	2 cut(s)	889, 1341
BsoBI	CYCGRG	1 cut(s)	839
Bsp1286I	GDGCHC	3 cut(s)	454, 713, 1861
Bsp143I	GATC	5 cut(s)	414, 853, 1343, 1379, 1492
Bsp19I	CCATGG	1 cut(s)	504
BspANI	GGCC	2 cut(s)	889, 1341
BspCNI	CTCAG	8 cut(s)	193, 469, 540, 553, 606, 1517, 1558, 1843
BspDI	ATCGAT	1 cut(s)	773
BspLI	GGNNCC	1 cut(s)	1608
BspMI	ACCTGC	1 cut(s)	1341
BspOI	GCTAGC	1 cut(s)	993
BspPI	GGATC	2 cut(s)	861, 1487
BsrDI	GCAATG	2 cut(s)	1234, 1377
BsrI	ACTGG	3 cut(s)	1648, 1862, 2015
BssECI	CCNNGG	4 cut(s)	36, 383, 504, 1365
BssMI	GATC	5 cut(s)	414, 853, 1343, 1379, 1492
BssNI	GRCGYC	1 cut(s)	652
BssSI	CACGAG	1 cut(s)	569
BssT1I	CCWWGG	1 cut(s)	504
Bst2BI	CACGAG	1 cut(s)	569
Bst2UI	CCWGG	3 cut(s)	38, 385, 1611
Bst4CI	ACNGT	1 cut(s)	997
Bst6I	CTCTTC	2 cut(s)	209, 534
BstACI	GRCGYC	1 cut(s)	652
BstAPI	GCANNNNNTGC	1 cut(s)	1236
BstBAI	YACGTR	3 cut(s)	427, 495, 1955
BstC8I	GCNNGC	3 cut(s)	226, 991, 1622
BstDEI	CTNAG	8 cut(s)	180, 477, 548, 561, 614, 1504, 1566, 1851
BstDSI	CCRYGG	1 cut(s)	504
BstF5I	GGATG	6 cut(s)	47, 99, 228, 358, 1297, 1530
BstKTI	GATC	5 cut(s)	417, 856, 1346, 1382, 1495
BstMAI	GTCTC	3 cut(s)	84, 339, 1184
BstMBI	GATC	5 cut(s)	414, 853, 1343, 1379, 1492
BstMWI	GCNNNNNNNGC	7 cut(s)	234, 821, 935, 980, 1236, 1347, 1457
BstNI	CCWGG	3 cut(s)	38, 385, 1611
BstNSI	RCATGY	4 cut(s)	228, 1030, 1323, 1434
BstSCI	CCNGG	4 cut(s)	36, 383, 647, 1609
BstSLI	GKGCMC	2 cut(s)	454, 1861
BstX2I	RGATCY	1 cut(s)	1492
BstYI	RGATCY	1 cut(s)	1492
Bsu15I	ATCGAT	1 cut(s)	773
Bsu36I	CCTNAGG	1 cut(s)	1851
BsuI	GTATCC	1 cut(s)	319
BsuRI	GGCC	2 cut(s)	889, 1341
BsuTUI	ATCGAT	1 cut(s)	773
BtgI	CCRYGG	1 cut(s)	504
BtsCI	GGATG	6 cut(s)	47, 99, 228, 358, 1297, 1530
BtsI	GCAGTG	2 cut(s)	613, 793
BtsIMutI	CAGTG	3 cut(s)	613, 793, 1893
BveI	ACCTGC	1 cut(s)	1341
Cac8I	GCNNGC	3 cut(s)	226, 991, 1622
ClaI	ATCGAT	1 cut(s)	773
CseI	GACGC	3 cut(s)	529, 641, 1497
Csp6I	GTAC	5 cut(s)	331, 424, 595, 1918, 1952
CviQI	GTAC	5 cut(s)	331, 424, 595, 1918, 1952
DdeI	CTNAG	8 cut(s)	180, 477, 548, 561, 614, 1504, 1566, 1851
DpnI	GATC	5 cut(s)	416, 855, 1345, 1381, 1494
DpnII	GATC	5 cut(s)	414, 853, 1343, 1379, 1492
DraI	TTTAAA	1 cut(s)	1981
DriI	GACNNNNNGTC	1 cut(s)	1507
EaeI	YGGCCR	1 cut(s)	1339
Eam1104I	CTCTTC	2 cut(s)	209, 534
Eam1105I	GACNNNNNGTC	1 cut(s)	1507
EarI	CTCTTC	2 cut(s)	209, 534
EciI	GGCGGA	1 cut(s)	58
Ecl136II	GAGCTC	1 cut(s)	711
Eco130I	CCWWGG	1 cut(s)	504
Eco147I	AGGCCT	1 cut(s)	889
Eco24I	GRGCYC	1 cut(s)	713
Eco32I	GATATC	1 cut(s)	1750
Eco53kI	GAGCTC	1 cut(s)	711
Eco57I	CTGAAG	1 cut(s)	1494
Eco81I	CCTNAGG	1 cut(s)	1851
Eco88I	CYCGRG	1 cut(s)	839
EcoICRI	GAGCTC	1 cut(s)	711
EcoRII	CCWGG	3 cut(s)	36, 383, 1609
EcoRV	GATATC	1 cut(s)	1750
EcoT14I	CCWWGG	1 cut(s)	504
EcoT22I	ATGCAT	1 cut(s)	230
EcoT38I	GRGCYC	1 cut(s)	713
ErhI	CCWWGG	1 cut(s)	504
FaqI	GGGAC	1 cut(s)	226
FauI	CCCGC	4 cut(s)	112, 674, 808, 1232
FauNDI	CATATG	1 cut(s)	723
FbaI	TGATCA	1 cut(s)	414
Fnu4HI	GCNGC	3 cut(s)	108, 111, 357
FokI	GGATG	6 cut(s)	34, 86, 215, 365, 1284, 1517
FriOI	GRGCYC	1 cut(s)	713
Fsp4HI	GCNGC	3 cut(s)	108, 111, 357
FspBI	CTAG	3 cut(s)	990, 1683, 1962
GluI	GCNGC	3 cut(s)	108, 111, 357
GsaI	CCCAGC	2 cut(s)	237, 987
HaeIII	GGCC	2 cut(s)	889, 1341
HapII	CCGG	1 cut(s)	648
HgaI	GACGC	3 cut(s)	529, 641, 1497
Hin1I	GRCGYC	1 cut(s)	652
HincII	GTYRAC	2 cut(s)	519, 1330
HindII	GTYRAC	2 cut(s)	519, 1330
HindIII	AAGCTT	1 cut(s)	1065
HinfI	GANTC	5 cut(s)	92, 550, 882, 1055, 1502
HpaII	CCGG	1 cut(s)	648
HphI	GGTGA	6 cut(s)	308, 449, 1018, 1637, 1673, 1882
Hpy166II	GTNNAC	5 cut(s)	519, 597, 661, 1330, 1859
Hpy188I	TCNGA	8 cut(s)	183, 193, 344, 562, 615, 1062, 1567, 1720
Hpy188III	TCNNGA	8 cut(s)	839, 1035, 1049, 1210, 1525, 1600, 1683, 1850
Hpy8I	GTNNAC	5 cut(s)	519, 597, 661, 1330, 1859
HpyAV	CCTTC	7 cut(s)	427, 451, 746, 1240, 1458, 1542, 1597
HpyCH4III	ACNGT	1 cut(s)	997
HpyCH4IV	ACGT	3 cut(s)	426, 494, 1954
HpyF10VI	GCNNNNNNNGC	7 cut(s)	234, 821, 935, 980, 1236, 1347, 1457
HpyF3I	CTNAG	8 cut(s)	180, 477, 548, 561, 614, 1504, 1566, 1851
HpySE526I	ACGT	3 cut(s)	426, 494, 1954
Hsp92I	GRCGYC	1 cut(s)	652
Ksp22I	TGATCA	1 cut(s)	414
Kzo9I	GATC	5 cut(s)	414, 853, 1343, 1379, 1492
LmnI	GCTCC	4 cut(s)	143, 267, 708, 1606
LweI	GCATC	9 cut(s)	237, 343, 555, 765, 844, 1081, 1390, 1665, 1948
MaeI	CTAG	3 cut(s)	990, 1683, 1962
MaeII	ACGT	3 cut(s)	426, 494, 1954
MaeIII	GTNAC	4 cut(s)	277, 508, 898, 1201
MalI	GATC	5 cut(s)	416, 855, 1345, 1381, 1494
MboI	GATC	5 cut(s)	414, 853, 1343, 1379, 1492
MboII	GAAGA	5 cut(s)	196, 551, 1193, 1487, 1888
MflI	RGATCY	1 cut(s)	1492
MhlI	GDGCHC	3 cut(s)	454, 713, 1861
MlyI	GAGTC	3 cut(s)	86, 559, 1496
MmeI	TCCRAC	2 cut(s)	322, 831
Mph1103I	ATGCAT	1 cut(s)	230
MroXI	GAANNNNTTC	1 cut(s)	1067
MslI	CAYNNNNRTG	4 cut(s)	286, 666, 1737, 1809
MspI	CCGG	1 cut(s)	648
MspR9I	CCNGG	4 cut(s)	38, 385, 649, 1611
Mva1269I	GAATGC	1 cut(s)	505
MvaI	CCWGG	3 cut(s)	38, 385, 1611
MwoI	GCNNNNNNNGC	7 cut(s)	234, 821, 935, 980, 1236, 1347, 1457
NciI	CCSGG	1 cut(s)	649
NcoI	CCATGG	1 cut(s)	504
NdeI	CATATG	1 cut(s)	723
NdeII	GATC	5 cut(s)	414, 853, 1343, 1379, 1492
NheI	GCTAGC	1 cut(s)	989
NlaIV	GGNNCC	1 cut(s)	1608
NmeAIII	GCCGAG	1 cut(s)	1390
NmuCI	GTSAC	3 cut(s)	277, 508, 898
NsiI	ATGCAT	1 cut(s)	230
NspI	RCATGY	4 cut(s)	228, 1030, 1323, 1434
OliI	CACNNNNGTG	1 cut(s)	1809
PaeI	GCATGC	1 cut(s)	228
PaeR7I	CTCGAG	1 cut(s)	839
PceI	AGGCCT	1 cut(s)	889
PciI	ACATGT	1 cut(s)	1319
PctI	GAATGC	1 cut(s)	505
PdmI	GAANNNNTTC	1 cut(s)	1067
PfeI	GAWTC	2 cut(s)	882, 1055
PflFI	GACNNNGTC	1 cut(s)	951
PkrI	GCNGC	3 cut(s)	109, 112, 358
PleI	GAGTC	3 cut(s)	86, 558, 1496
PpsI	GAGTC	3 cut(s)	86, 558, 1496
Ppu21I	YACGTR	3 cut(s)	427, 495, 1955
PscI	ACATGT	1 cut(s)	1319
Psp124BI	GAGCTC	1 cut(s)	713
Psp6I	CCWGG	3 cut(s)	36, 383, 1609
PspFI	CCCAGC	2 cut(s)	233, 983
PspGI	CCWGG	3 cut(s)	36, 383, 1609
PspN4I	GGNNCC	1 cut(s)	1608
PsuI	RGATCY	1 cut(s)	1492
PsyI	GACNNNGTC	1 cut(s)	951
RsaI	GTAC	5 cut(s)	332, 425, 596, 1919, 1953
RsaNI	GTAC	5 cut(s)	331, 424, 595, 1918, 1952
RseI	CAYNNNNRTG	4 cut(s)	286, 666, 1737, 1809
SacI	GAGCTC	1 cut(s)	713
SatI	GCNGC	3 cut(s)	108, 111, 357
Sau3AI	GATC	5 cut(s)	414, 853, 1343, 1379, 1492
SchI	GAGTC	3 cut(s)	86, 559, 1496
ScrFI	CCNGG	4 cut(s)	38, 385, 649, 1611
SduI	GDGCHC	3 cut(s)	454, 713, 1861
SfaNI	GCATC	9 cut(s)	237, 343, 555, 765, 844, 1081, 1390, 1665, 1948
Sfr274I	CTCGAG	1 cut(s)	839
SlaI	CTCGAG	1 cut(s)	839
SmiMI	CAYNNNNRTG	4 cut(s)	286, 666, 1737, 1809
SmlI	CTYRAG	3 cut(s)	839, 1210, 1259
SmoI	CTYRAG	3 cut(s)	839, 1210, 1259
SphI	GCATGC	1 cut(s)	228
SseBI	AGGCCT	1 cut(s)	889
SspMI	CTAG	3 cut(s)	990, 1683, 1962
SstI	GAGCTC	1 cut(s)	713
StuI	AGGCCT	1 cut(s)	889
StyD4I	CCNGG	4 cut(s)	36, 383, 647, 1609
StyI	CCWWGG	1 cut(s)	504
TaaI	ACNGT	1 cut(s)	997
TaiI	ACGT	3 cut(s)	429, 497, 1957
TaqI	TCGA	7 cut(s)	95, 773, 840, 1034, 1281, 1526, 1546
TatI	WGTACW	3 cut(s)	330, 594, 1917
TauI	GCSGC	3 cut(s)	110, 113, 359
TfiI	GAWTC	2 cut(s)	882, 1055
TscAI	CASTG	3 cut(s)	613, 793, 1900
TseFI	GTSAC	3 cut(s)	277, 508, 898
Tsp45I	GTSAC	3 cut(s)	277, 508, 898
TspRI	CASTG	3 cut(s)	613, 793, 1900
Tth111I	GACNNNGTC	1 cut(s)	951
VneI	GTGCAC	1 cut(s)	1857
XapI	RAATTY	4 cut(s)	530, 960, 1641, 1879
XbaI	TCTAGA	1 cut(s)	1682
XceI	RCATGY	4 cut(s)	228, 1030, 1323, 1434
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1617
XhoI	CTCGAG	1 cut(s)	839
XmnI	GAANNNNTTC	1 cut(s)	1067
XspI	CTAG	3 cut(s)	990, 1683, 1962
Zsp2I	ATGCAT	1 cut(s)	230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.