Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| prunus_persica | Prupe.2G096200_v2.0.a1 Prupe.6G189400_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr4g0396371 |
| rosa_rugosa | Rorug03G0349500 |
| rosa_samantha | Rh4AG067700 Rh4CG073600 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 177 |
| AcsI | RAATTY | 1 cut(s) | 105 |
| AgsI | TTSAA | 2 cut(s) | 47, 92 |
| AjnI | CCWGG | 1 cut(s) | 331 |
| AluBI | AGCT | 2 cut(s) | 57, 285 |
| AluI | AGCT | 2 cut(s) | 57, 285 |
| Alw21I | GWGCWC | 1 cut(s) | 287 |
| ApoI | RAATTY | 1 cut(s) | 105 |
| AspS9I | GGNCC | 1 cut(s) | 162 |
| AvaII | GGWCC | 1 cut(s) | 162 |
| BanII | GRGCYC | 1 cut(s) | 287 |
| BbsI | GAAGAC | 1 cut(s) | 38 |
| Bbv12I | GWGCWC | 1 cut(s) | 287 |
| BccI | CCATC | 2 cut(s) | 149, 272 |
| BciT130I | CCWGG | 1 cut(s) | 333 |
| BfaI | CTAG | 2 cut(s) | 149, 343 |
| BglII | AGATCT | 2 cut(s) | 6, 234 |
| Bme1390I | CCNGG | 1 cut(s) | 333 |
| Bme18I | GGWCC | 1 cut(s) | 162 |
| BmgT120I | GGNCC | 1 cut(s) | 162 |
| BmiI | GGNNCC | 1 cut(s) | 164 |
| BmrFI | CCNGG | 1 cut(s) | 333 |
| BmsI | GCATC | 1 cut(s) | 211 |
| BpiI | GAAGAC | 1 cut(s) | 38 |
| BsaJI | CCNNGG | 1 cut(s) | 332 |
| Bse1I | ACTGG | 1 cut(s) | 259 |
| BseBI | CCWGG | 1 cut(s) | 333 |
| BseDI | CCNNGG | 1 cut(s) | 332 |
| BseGI | GGATG | 2 cut(s) | 160, 180 |
| BseNI | ACTGG | 1 cut(s) | 259 |
| BsiHKAI | GWGCWC | 1 cut(s) | 287 |
| Bsp1286I | GDGCHC | 1 cut(s) | 287 |
| Bsp143I | GATC | 2 cut(s) | 6, 234 |
| BspLI | GGNNCC | 1 cut(s) | 164 |
| BsrI | ACTGG | 1 cut(s) | 259 |
| BssECI | CCNNGG | 1 cut(s) | 332 |
| BssMI | GATC | 2 cut(s) | 6, 234 |
| Bst2UI | CCWGG | 1 cut(s) | 333 |
| BstF5I | GGATG | 2 cut(s) | 160, 180 |
| BstKTI | GATC | 2 cut(s) | 9, 237 |
| BstMBI | GATC | 2 cut(s) | 6, 234 |
| BstNI | CCWGG | 1 cut(s) | 333 |
| BstSCI | CCNGG | 1 cut(s) | 331 |
| BstV2I | GAAGAC | 1 cut(s) | 38 |
| BstX2I | RGATCY | 2 cut(s) | 6, 234 |
| BstYI | RGATCY | 2 cut(s) | 6, 234 |
| BtsCI | GGATG | 2 cut(s) | 160, 180 |
| Cfr13I | GGNCC | 1 cut(s) | 162 |
| CviJI | RGCY | 4 cut(s) | 57, 127, 285, 325 |
| CviKI_1 | RGCY | 4 cut(s) | 57, 127, 285, 325 |
| DpnI | GATC | 2 cut(s) | 8, 236 |
| DpnII | GATC | 2 cut(s) | 6, 234 |
| Ecl136II | GAGCTC | 1 cut(s) | 285 |
| Eco24I | GRGCYC | 1 cut(s) | 287 |
| Eco47I | GGWCC | 1 cut(s) | 162 |
| Eco53kI | GAGCTC | 1 cut(s) | 285 |
| EcoICRI | GAGCTC | 1 cut(s) | 285 |
| EcoO109I | RGGNCCY | 1 cut(s) | 162 |
| EcoRI | GAATTC | 1 cut(s) | 105 |
| EcoRII | CCWGG | 1 cut(s) | 331 |
| EcoT38I | GRGCYC | 1 cut(s) | 287 |
| FaiI | YATR | 5 cut(s) | 68, 131, 177, 230, 296 |
| FokI | GGATG | 2 cut(s) | 167, 167 |
| FriOI | GRGCYC | 1 cut(s) | 287 |
| FspBI | CTAG | 2 cut(s) | 149, 343 |
| HincII | GTYRAC | 1 cut(s) | 18 |
| HindII | GTYRAC | 1 cut(s) | 18 |
| HinfI | GANTC | 2 cut(s) | 88, 191 |
| Hpy166II | GTNNAC | 1 cut(s) | 18 |
| Hpy188I | TCNGA | 1 cut(s) | 289 |
| Hpy188III | TCNNGA | 3 cut(s) | 92, 184, 272 |
| Hpy8I | GTNNAC | 1 cut(s) | 18 |
| HpyAV | CCTTC | 1 cut(s) | 181 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 202, 232 |
| Kzo9I | GATC | 2 cut(s) | 6, 234 |
| LpnPI | CCDG | 7 cut(s) | 12, 123, 126, 131, 197, 240, 318 |
| LweI | GCATC | 1 cut(s) | 211 |
| MaeI | CTAG | 2 cut(s) | 149, 343 |
| MalI | GATC | 2 cut(s) | 8, 236 |
| MboI | GATC | 2 cut(s) | 6, 234 |
| MboII | GAAGA | 1 cut(s) | 43 |
| MflI | RGATCY | 2 cut(s) | 6, 234 |
| MhlI | GDGCHC | 1 cut(s) | 287 |
| MluCI | AATT | 1 cut(s) | 105 |
| MnlI | CCTC | 4 cut(s) | 94, 117, 145, 215 |
| MseI | TTAA | 1 cut(s) | 243 |
| MspR9I | CCNGG | 1 cut(s) | 333 |
| MvaI | CCWGG | 1 cut(s) | 333 |
| NdeII | GATC | 2 cut(s) | 6, 234 |
| NlaIV | GGNNCC | 1 cut(s) | 164 |
| PfeI | GAWTC | 2 cut(s) | 88, 191 |
| PflFI | GACNNNGTC | 1 cut(s) | 74 |
| PpuMI | RGGWCCY | 1 cut(s) | 162 |
| PsiI | TTATAA | 1 cut(s) | 177 |
| Psp124BI | GAGCTC | 1 cut(s) | 287 |
| Psp5II | RGGWCCY | 1 cut(s) | 162 |
| Psp6I | CCWGG | 1 cut(s) | 331 |
| PspGI | CCWGG | 1 cut(s) | 331 |
| PspN4I | GGNNCC | 1 cut(s) | 164 |
| PspPI | GGNCC | 1 cut(s) | 162 |
| PspPPI | RGGWCCY | 1 cut(s) | 162 |
| PsuI | RGATCY | 2 cut(s) | 6, 234 |
| PsyI | GACNNNGTC | 1 cut(s) | 74 |
| SacI | GAGCTC | 1 cut(s) | 287 |
| SaqAI | TTAA | 1 cut(s) | 243 |
| Sau3AI | GATC | 2 cut(s) | 6, 234 |
| Sau96I | GGNCC | 1 cut(s) | 162 |
| ScrFI | CCNGG | 1 cut(s) | 333 |
| SduI | GDGCHC | 1 cut(s) | 287 |
| SetI | ASST | 5 cut(s) | 59, 200, 226, 251, 287 |
| SfaNI | GCATC | 1 cut(s) | 211 |
| SinI | GGWCC | 1 cut(s) | 162 |
| Sse9I | AATT | 1 cut(s) | 105 |
| SspMI | CTAG | 2 cut(s) | 149, 343 |
| SstI | GAGCTC | 1 cut(s) | 287 |
| StyD4I | CCNGG | 1 cut(s) | 331 |
| TasI | AATT | 1 cut(s) | 105 |
| TfiI | GAWTC | 2 cut(s) | 88, 191 |
| Tru1I | TTAA | 1 cut(s) | 243 |
| Tru9I | TTAA | 1 cut(s) | 243 |
| TspGWI | ACGGA | 1 cut(s) | 203 |
| Tth111I | GACNNNGTC | 1 cut(s) | 74 |
| VpaK11BI | GGWCC | 1 cut(s) | 162 |
| XapI | RAATTY | 1 cut(s) | 105 |
| XspI | CTAG | 2 cut(s) | 149, 343 |