Prupe.2G218500_v2.0.a1

mRNA splicing, via spliceosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0022450
Physical Location & Seq
Reverse (-)
24792609 .. 24793131
523 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G218500.1

Sequence Viewer

Length: 333 bp
ATGGCATTGGCAGCCCAGCCTAAGAACCTGCAGATTGAGAATGTTGTTTTGAACCTTCCTAGGAACATTCGGTGCAACAAGTGTAGGAATACCATGGCCAAAGACACCAAGATCAATTCCCGCAAAGAAAAATCCTCCGGCAACTTGGAGTATTTGGGGATTAAAAACATCAGATTTCACTTCAATTGTACCCAGTGCTCGGCTGGGATCGTCTTAAGGACAAACCCGCAATTGTCACGCGTTCTTTGTGAGTCCGATGCTACACTATTCTGTGGTGATCAACTTCCCGGACGTCTTCCGTATGGGCTTGAGGAGGTAAAAAAAAGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

111

Amino Acids

12.42

Weight (kDa)

9.61

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022450)

Species Orthologous Gene IDs
prunus_persica Prupe.2G218500_v2.0.a1 Prupe.3G160000_v2.0.a1 Prupe.4G012700_v2.0.a1 Prupe.4G015800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 295
Acc36I ACCTGC 1 cut(s) 36
AccII CGCG 1 cut(s) 240
AciI CCGC 2 cut(s) 121, 227
AclWI GGATC 1 cut(s) 215
AcoI YGGCCR 1 cut(s) 96
AcyI GRCGYC 1 cut(s) 292
AfaI GTAC 1 cut(s) 190
AfiI CCNNNNNNNGG 1 cut(s) 199
AflII CTTAAG 1 cut(s) 214
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 2 cut(s) 52, 184
Alw21I GWGCWC 1 cut(s) 200
AlwI GGATC 1 cut(s) 215
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 11
AspA2I CCTAGG 1 cut(s) 59
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 287
AvrII CCTAGG 1 cut(s) 59
BalI TGGCCA 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 23
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 288
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 1 cut(s) 29
BfrI CTTAAG 1 cut(s) 214
BfuAI ACCTGC 1 cut(s) 36
BisI GCNGC 1 cut(s) 12
BlnI CCTAGG 1 cut(s) 59
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 288
BmrFI CCNGG 1 cut(s) 288
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 1 cut(s) 247
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 288
BsaHI GRCGYC 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 59, 93
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 59, 93
BseLI CCNNNNNNNGG 1 cut(s) 199
BseNI ACTGG 1 cut(s) 193
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 1 cut(s) 23
BseYI CCCAGC 2 cut(s) 15, 203
Bsh1236I CGCG 1 cut(s) 240
BshFI GGCC 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 200
BsiSI CCGG 2 cut(s) 138, 288
BslI CCNNNNNNNGG 1 cut(s) 199
BsnI GGCC 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 3 cut(s) 111, 207, 277
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 2 cut(s) 121, 227
BspANI GGCC 1 cut(s) 98
BspFNI CGCG 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 36
BspPI GGATC 1 cut(s) 215
BspTI CTTAAG 1 cut(s) 214
BsrI ACTGG 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 59, 93
BssMI GATC 3 cut(s) 111, 207, 277
BssNI GRCGYC 1 cut(s) 292
BssT1I CCWWGG 2 cut(s) 59, 93
BstACI GRCGYC 1 cut(s) 292
BstAFI CTTAAG 1 cut(s) 214
BstDEI CTNAG 1 cut(s) 21
BstDSI CCRYGG 1 cut(s) 93
BstFNI CGCG 1 cut(s) 240
BstKTI GATC 3 cut(s) 114, 210, 280
BstMBI GATC 3 cut(s) 111, 207, 277
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 1 cut(s) 29
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 287
BsuRI GGCC 1 cut(s) 98
BtgI CCRYGG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 200
BveI ACCTGC 1 cut(s) 36
Csp6I GTAC 1 cut(s) 189
CviAII CATG 1 cut(s) 94
CviJI RGCY 5 cut(s) 14, 19, 98, 203, 307
CviKI_1 RGCY 5 cut(s) 14, 19, 98, 203, 307
CviQI GTAC 1 cut(s) 189
DdeI CTNAG 1 cut(s) 21
DpnI GATC 3 cut(s) 113, 209, 279
DpnII GATC 3 cut(s) 111, 207, 277
EaeI YGGCCR 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 59, 93
EcoT14I CCWWGG 2 cut(s) 59, 93
ErhI CCWWGG 2 cut(s) 59, 93
FaeI CATG 1 cut(s) 97
FaiI YATR 2 cut(s) 95, 303
FatI CATG 1 cut(s) 93
FauI CCCGC 2 cut(s) 128, 234
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 1 cut(s) 12
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 1 cut(s) 60
GluI GCNGC 1 cut(s) 12
GsaI CCCAGC 2 cut(s) 19, 207
HaeIII GGCC 1 cut(s) 98
HapII CCGG 2 cut(s) 138, 288
Hin1I GRCGYC 1 cut(s) 292
Hin1II CATG 1 cut(s) 97
HinfI GANTC 1 cut(s) 251
HpaII CCGG 2 cut(s) 138, 288
HphI GGTGA 1 cut(s) 287
Hpy188I TCNGA 2 cut(s) 173, 256
HpyAV CCTTC 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 2 cut(s) 31, 75
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 21
HpySE526I ACGT 1 cut(s) 292
Hsp92I GRCGYC 1 cut(s) 292
Hsp92II CATG 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 3 cut(s) 111, 207, 277
LpnPI CCDG 6 cut(s) 29, 41, 151, 189, 206, 301
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 247
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 1 cut(s) 234
MalI GATC 3 cut(s) 113, 209, 279
MboI GATC 3 cut(s) 111, 207, 277
MboII GAAGA 1 cut(s) 287
MfeI CAATTG 2 cut(s) 184, 230
MhlI GDGCHC 1 cut(s) 200
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 3 cut(s) 115, 184, 230
MluI ACGCGT 1 cut(s) 238
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 3 cut(s) 145, 304, 307
Mox20I TGGCCA 1 cut(s) 98
MscI TGGCCA 1 cut(s) 98
MseI TTAA 2 cut(s) 162, 215
Msp20I TGGCCA 1 cut(s) 98
MspCI CTTAAG 1 cut(s) 214
MspI CCGG 2 cut(s) 138, 288
MspR9I CCNGG 1 cut(s) 288
MunI CAATTG 2 cut(s) 184, 230
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 288
NcoI CCATGG 1 cut(s) 93
NdeII GATC 3 cut(s) 111, 207, 277
NlaIII CATG 1 cut(s) 97
NmeAIII GCCGAG 1 cut(s) 179
NmuCI GTSAC 1 cut(s) 234
PfoI TCCNGGA 1 cut(s) 286
PkrI GCNGC 1 cut(s) 13
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PspFI CCCAGC 2 cut(s) 15, 203
PstI CTGCAG 1 cut(s) 33
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
SaqAI TTAA 2 cut(s) 162, 215
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 3 cut(s) 111, 207, 277
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 288
SduI GDGCHC 1 cut(s) 200
SetI ASST 4 cut(s) 30, 57, 295, 318
SfaNI GCATC 1 cut(s) 247
SfcI CTRYAG 1 cut(s) 29
SmlI CTYRAG 2 cut(s) 214, 308
SmoI CTYRAG 2 cut(s) 214, 308
Sse9I AATT 3 cut(s) 115, 184, 230
SsiI CCGC 2 cut(s) 121, 227
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 2 cut(s) 59, 93
TaiI ACGT 1 cut(s) 295
TasI AATT 3 cut(s) 115, 184, 230
Tru1I TTAA 2 cut(s) 162, 215
Tru9I TTAA 2 cut(s) 162, 215
TscAI CASTG 1 cut(s) 200
TseFI GTSAC 1 cut(s) 234
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 234
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 1 cut(s) 200
Vha464I CTTAAG 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XmaJI CCTAGG 1 cut(s) 59
XspI CTAG 1 cut(s) 60
ZraI GACGTC 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.