Prupe.4G012700_v2.0.a1

mRNA splicing, via spliceosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0022450
Physical Location & Seq
Forward (+)
601061 .. 602530
1470 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G012700.1

Sequence Viewer

Length: 381 bp
ATGGCGGCCCGGCGTAAGAACCCACAGATTGAGAATGTTCTGAAGATGCTTCCCTCGAGCATTCGCTGCACCGCTTGTAGCACTGTCATGGCCAAAGACACCAAGTTGAATTCCCGCAAAGAAGAAGCCCTCGGCGAGGAGTTTTGGGAGGTTTGGAAGGATCACACATTCAGATTTTGCTTCAGTTGTACCCAGTGCTCGGCTGAGATCGTCATAAGGTCAGACCCACAATATTCACACCTTACTGTTGAGGCCGGTGCTTCGAAATTTTATAGTCATTTTATCCCTTGGCATCCCCCTAGGGTTGAGGAGATACAACAAATTCAGAAAAGGAAGATTGAAGAAGCGGGAGATGCCATGAAATCTTTGGATTTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

127

Amino Acids

14.63

Weight (kDa)

8.29

Isoelectric Point (pI)

62.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022450)

Species Orthologous Gene IDs
prunus_persica Prupe.2G218500_v2.0.a1 Prupe.3G160000_v2.0.a1 Prupe.4G012700_v2.0.a1 Prupe.4G015800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 72, 115, 347
AclWI GGATC 1 cut(s) 168
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 3 cut(s) 109, 266, 321
AcuI CTGAAG 2 cut(s) 62, 166
AfaI GTAC 1 cut(s) 190
AfiI CCNNNNNNNGG 2 cut(s) 136, 199
AgsI TTSAA 3 cut(s) 109, 341, 376
Alw21I GWGCWC 1 cut(s) 200
AlwI GGATC 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 55
AoxI GGCC 3 cut(s) 6, 90, 252
ApeKI GCWGC 1 cut(s) 66
ApoI RAATTY 3 cut(s) 109, 266, 321
AspA2I CCTAGG 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 10
AsuII TTCGAA 1 cut(s) 263
AvaI CYCGRG 1 cut(s) 55
AvrII CCTAGG 1 cut(s) 299
BalI TGGCCA 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 53
BcnI CCSGG 1 cut(s) 10
BfaI CTAG 1 cut(s) 300
BisI GCNGC 2 cut(s) 6, 67
BlnI CCTAGG 1 cut(s) 299
BlsI GCNGC 2 cut(s) 7, 68
Bme1390I CCNGG 1 cut(s) 10
BmeT110I CYCGRG 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 7
BmrFI CCNGG 1 cut(s) 10
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 3 cut(s) 36, 301, 343
BmuI ACTGGG 1 cut(s) 187
Bpu14I TTCGAA 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 10
BsaJI CCNNGG 3 cut(s) 130, 287, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 199
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 193
BseDI CCNNGG 3 cut(s) 130, 287, 299
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 2 cut(s) 136, 199
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 193
BseRI GAGGAG 2 cut(s) 152, 323
BseXI GCAGC 1 cut(s) 53
BsgI GTGCAG 1 cut(s) 52
BshFI GGCC 3 cut(s) 8, 92, 254
BsiHKAI GWGCWC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 55
BsiSI CCGG 2 cut(s) 10, 255
BslI CCNNNNNNNGG 2 cut(s) 136, 199
BsmI GAATGC 1 cut(s) 60
BsnI GGCC 3 cut(s) 8, 92, 254
BsoBI CYCGRG 1 cut(s) 55
Bsp119I TTCGAA 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 2 cut(s) 160, 207
BspACI CCGC 4 cut(s) 5, 72, 115, 347
BspANI GGCC 3 cut(s) 8, 92, 254
BspCNI CTCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 168
BspT104I TTCGAA 1 cut(s) 263
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 1 cut(s) 193
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 3 cut(s) 130, 287, 299
BssMI GATC 2 cut(s) 160, 207
BssT1I CCWWGG 2 cut(s) 287, 299
Bst4CI ACNGT 2 cut(s) 85, 247
BstAPI GCANNNNNTGC 1 cut(s) 66
BstBI TTCGAA 1 cut(s) 263
BstDEI CTNAG 1 cut(s) 204
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 1 cut(s) 292
BstKTI GATC 2 cut(s) 163, 210
BstMBI GATC 2 cut(s) 160, 207
BstMWI GCNNNNNNNGC 2 cut(s) 66, 353
BstSCI CCNGG 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 53
BsuRI GGCC 3 cut(s) 8, 92, 254
BtsCI GGATG 1 cut(s) 292
BtsIMutI CAGTG 2 cut(s) 81, 200
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 1 cut(s) 7
Csp6I GTAC 1 cut(s) 189
CviAII CATG 2 cut(s) 88, 358
CviJI RGCY 5 cut(s) 8, 92, 128, 203, 254
CviKI_1 RGCY 5 cut(s) 8, 92, 128, 203, 254
CviQI GTAC 1 cut(s) 189
DdeI CTNAG 1 cut(s) 204
DpnI GATC 2 cut(s) 162, 209
DpnII GATC 2 cut(s) 160, 207
EaeI YGGCCR 1 cut(s) 90
Eco130I CCWWGG 2 cut(s) 287, 299
Eco57I CTGAAG 2 cut(s) 62, 166
Eco88I CYCGRG 1 cut(s) 55
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 287, 299
ErhI CCWWGG 2 cut(s) 287, 299
FaeI CATG 2 cut(s) 91, 361
FaiI YATR 4 cut(s) 89, 215, 273, 359
FatI CATG 2 cut(s) 87, 357
FauI CCCGC 2 cut(s) 122, 340
Fnu4HI GCNGC 2 cut(s) 6, 67
FokI GGATG 1 cut(s) 279
Fsp4HI GCNGC 2 cut(s) 6, 67
FspBI CTAG 1 cut(s) 300
GluI GCNGC 2 cut(s) 6, 67
HaeIII GGCC 3 cut(s) 8, 92, 254
HapII CCGG 2 cut(s) 10, 255
Hin1II CATG 2 cut(s) 91, 361
HpaII CCGG 2 cut(s) 10, 255
Hpy188I TCNGA 4 cut(s) 42, 173, 223, 327
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 2 cut(s) 85, 247
HpyCH4V TGCA 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 353
HpyF3I CTNAG 1 cut(s) 204
Hsp92II CATG 2 cut(s) 91, 361
Kzo9I GATC 2 cut(s) 160, 207
LpnPI CCDG 3 cut(s) 23, 206, 268
Lsp1109I GCAGC 1 cut(s) 53
LweI GCATC 3 cut(s) 36, 301, 343
MaeI CTAG 1 cut(s) 300
MalI GATC 2 cut(s) 162, 209
MboI GATC 2 cut(s) 160, 207
MboII GAAGA 4 cut(s) 55, 134, 346, 353
MhlI GDGCHC 1 cut(s) 200
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 3 cut(s) 109, 266, 321
MluNI TGGCCA 1 cut(s) 92
MnlI CCTC 6 cut(s) 64, 130, 140, 142, 244, 301
Mox20I TGGCCA 1 cut(s) 92
MscI TGGCCA 1 cut(s) 92
MslI CAYNNNNRTG 1 cut(s) 86
Msp20I TGGCCA 1 cut(s) 92
MspI CCGG 2 cut(s) 10, 255
MspR9I CCNGG 1 cut(s) 10
Mva1269I GAATGC 1 cut(s) 60
MwoI GCNNNNNNNGC 2 cut(s) 66, 353
NciI CCSGG 1 cut(s) 10
NdeII GATC 2 cut(s) 160, 207
NlaIII CATG 2 cut(s) 91, 361
NmeAIII GCCGAG 2 cut(s) 111, 179
NspV TTCGAA 1 cut(s) 263
PaeR7I CTCGAG 1 cut(s) 55
PctI GAATGC 1 cut(s) 60
PkrI GCNGC 2 cut(s) 7, 68
PspPI GGNCC 1 cut(s) 7
PspXI VCTCGAGB 1 cut(s) 55
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
RseI CAYNNNNRTG 1 cut(s) 86
SatI GCNGC 2 cut(s) 6, 67
Sau3AI GATC 2 cut(s) 160, 207
Sau96I GGNCC 1 cut(s) 7
ScrFI CCNGG 1 cut(s) 10
SduI GDGCHC 1 cut(s) 200
SetI ASST 3 cut(s) 153, 221, 243
SfaNI GCATC 3 cut(s) 36, 301, 343
Sfr274I CTCGAG 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 263
SlaI CTCGAG 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
Sse9I AATT 3 cut(s) 109, 266, 321
SsiI CCGC 4 cut(s) 5, 72, 115, 347
SspI AATATT 1 cut(s) 233
SspMI CTAG 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 8
StyI CCWWGG 2 cut(s) 287, 299
TaaI ACNGT 2 cut(s) 85, 247
TaqI TCGA 2 cut(s) 56, 263
TasI AATT 3 cut(s) 109, 266, 321
TauI GCSGC 1 cut(s) 8
TscAI CASTG 2 cut(s) 88, 200
TseI GCWGC 1 cut(s) 66
TspDTI ATGAA 1 cut(s) 374
TspRI CASTG 2 cut(s) 88, 200
XagI CCTNNNNNAGG 1 cut(s) 134
XapI RAATTY 3 cut(s) 109, 266, 321
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XhoI CTCGAG 1 cut(s) 55
XmaJI CCTAGG 1 cut(s) 299
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.