Prupe.3G000600_v2.0.a1

Germin-like protein subfamily 2 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0021895
Physical Location & Seq
Reverse (-)
78305 .. 79916
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G000600.1

Sequence Viewer

Length: 669 bp
ATGGCTGCCAACTTTGTTGCATATCTAGCCATCTTCACATTCACTGCTGCGTTCTCCGGTGCACTTGCATCGGACCCGGACTTGCTTCAAGATGTCTGCGTGGCTCTACCATCTTCTTCAGGGGGGGTAAAAGTGAACGGGTTTGCATGCAAGGATGATGCAAATGTGACGGCAGCTGATTTCTTCTTCGACGGCCTTGCGAAGCCAGGGCTGATCAACAACTCAGTGGGCTCTATCGTAACTGCAGCTAATGTGGAAAAGGTTCCAGGCCTCAACACACTGGGCGTGTCCCTGTCGCGCATCGACTATGCACCTGGTGGACTTAACCCACCTCACACCCATCAGCGTGCCACTGAGATAGTATTTGTGCTGGTCGGCGAATTGGATGTGGGATTCATCACCACAGCCAACAAGCTGGTTTCCAAGACCATCAACAAAGGCGAGATCTTTGTGTTTCCTAGGGGTCTGGTTCACTTCCAGAAGAACAACGGGGACAAGGCCGCTGCTGTAATTGCTGCCTTCAACAGCCAATTGCCTGGTACTCAATCCATCCCTGCAGCATTGTTCACGGCAACACCTCCTGTTCCTGACGAGGTCTTGACCAAAGCCTTCCAGCTTGGTACCAAGGAGATTGACAAGATCAAATCCAAGCTTGCTCCAAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

223

Amino Acids

23.13

Weight (kDa)

6.82

Isoelectric Point (pI)

24.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02335 AT3G62020 AT3G62020
prunus_persica Prupe.3G000600_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 620
AccB1I GGYRCC 1 cut(s) 620
AccII CGCG 1 cut(s) 298
AciI CCGC 1 cut(s) 501
AcuI CTGAAG 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 317
AfaI GTAC 2 cut(s) 541, 622
AgsI TTSAA 2 cut(s) 89, 523
AjnI CCWGG 4 cut(s) 205, 265, 313, 535
AluBI AGCT 5 cut(s) 176, 248, 415, 616, 652
AluI AGCT 5 cut(s) 176, 248, 415, 616, 652
Alw21I GWGCWC 1 cut(s) 64
Alw44I GTGCAC 1 cut(s) 60
AoxI GGCC 3 cut(s) 193, 268, 498
ApaLI GTGCAC 1 cut(s) 60
ApeKI GCWGC 7 cut(s) 5, 47, 173, 245, 503, 515, 557
Asp700I GAANNNNTTC 1 cut(s) 261
Asp718I GGTACC 1 cut(s) 620
AspA2I CCTAGG 1 cut(s) 458
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 73
AsuC2I CCSGG 1 cut(s) 77
AsuHPI GGTGA 1 cut(s) 391
AvaII GGWCC 1 cut(s) 73
AvrII CCTAGG 1 cut(s) 458
BaeGI GKGCMC 1 cut(s) 64
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 64
BbvI GCAGC 6 cut(s) 34, 185, 257, 490, 502, 569
BccI CCATC 5 cut(s) 38, 118, 348, 437, 557
BceAI ACGGC 3 cut(s) 186, 208, 585
BcgI CGANNNNNNTGC 4 cut(s) 51, 85, 179, 213
BciT130I CCWGG 4 cut(s) 207, 267, 315, 537
BclI TGATCA 1 cut(s) 213
BcnI CCSGG 1 cut(s) 77
BfaI CTAG 2 cut(s) 26, 459
BfmI CTRYAG 2 cut(s) 243, 555
BglII AGATCT 1 cut(s) 444
BisI GCNGC 8 cut(s) 6, 48, 174, 246, 501, 504, 516, 558
BlnI CCTAGG 1 cut(s) 458
BlsI GCNGC 8 cut(s) 7, 49, 175, 247, 502, 505, 517, 559
Bme1390I CCNGG 5 cut(s) 77, 207, 267, 315, 537
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmiI GGNNCC 3 cut(s) 75, 264, 622
BmrFI CCNGG 5 cut(s) 77, 207, 267, 315, 537
BmrI ACTGGG 1 cut(s) 290
BmsI GCATC 3 cut(s) 77, 148, 309
BmuI ACTGGG 1 cut(s) 290
BpuMI CCSGG 1 cut(s) 77
BsaJI CCNNGG 3 cut(s) 206, 458, 624
BsaWI WCCGGW 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 285
BseBI CCWGG 4 cut(s) 207, 267, 315, 537
BseDI CCNNGG 3 cut(s) 206, 458, 624
BseGI GGATG 3 cut(s) 160, 391, 549
BseMII CTCAG 2 cut(s) 237, 345
BseNI ACTGG 1 cut(s) 285
BseSI GKGCMC 1 cut(s) 64
BseXI GCAGC 6 cut(s) 34, 185, 257, 490, 502, 569
Bsh1236I CGCG 1 cut(s) 298
BshFI GGCC 3 cut(s) 195, 270, 500
BshNI GGYRCC 1 cut(s) 620
BsiHKAI GWGCWC 1 cut(s) 64
BsiSI CCGG 2 cut(s) 57, 77
BslFI GGGAC 2 cut(s) 274, 506
BsmFI GGGAC 2 cut(s) 274, 506
BsnI GGCC 3 cut(s) 195, 270, 500
Bsp1286I GDGCHC 2 cut(s) 64, 233
Bsp143I GATC 3 cut(s) 213, 444, 639
BspACI CCGC 1 cut(s) 501
BspANI GGCC 3 cut(s) 195, 270, 500
BspCNI CTCAG 2 cut(s) 236, 346
BspFNI CGCG 1 cut(s) 298
BspLI GGNNCC 3 cut(s) 75, 264, 622
BspMAI CTGCAG 2 cut(s) 247, 559
BspT107I GGYRCC 1 cut(s) 620
BsrI ACTGG 1 cut(s) 285
BssECI CCNNGG 3 cut(s) 206, 458, 624
BssMI GATC 3 cut(s) 213, 444, 639
BssT1I CCWWGG 2 cut(s) 458, 624
Bst2UI CCWGG 4 cut(s) 207, 267, 315, 537
BstC8I GCNNGC 3 cut(s) 148, 348, 654
BstDEI CTNAG 2 cut(s) 223, 354
BstF5I GGATG 3 cut(s) 160, 391, 549
BstFNI CGCG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 3 cut(s) 216, 447, 642
BstMBI GATC 3 cut(s) 213, 444, 639
BstMWI GCNNNNNNNGC 2 cut(s) 26, 512
BstNI CCWGG 4 cut(s) 207, 267, 315, 537
BstNSI RCATGY 1 cut(s) 150
BstSCI CCNGG 5 cut(s) 75, 205, 265, 313, 535
BstSFI CTRYAG 2 cut(s) 243, 555
BstSLI GKGCMC 1 cut(s) 64
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 6 cut(s) 34, 185, 257, 490, 502, 569
BstX2I RGATCY 1 cut(s) 444
BstXI CCANNNNNNTGG 2 cut(s) 415, 536
BstYI RGATCY 1 cut(s) 444
BsuRI GGCC 3 cut(s) 195, 270, 500
BtsCI GGATG 3 cut(s) 160, 391, 549
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 4 cut(s) 42, 231, 278, 351
Cac8I GCNNGC 3 cut(s) 148, 348, 654
CfoI GCGC 1 cut(s) 300
Cfr13I GGNCC 1 cut(s) 73
CsiI ACCWGGT 1 cut(s) 313
Csp6I GTAC 2 cut(s) 540, 621
CviAII CATG 1 cut(s) 147
CviQI GTAC 2 cut(s) 540, 621
DdeI CTNAG 2 cut(s) 223, 354
DpnI GATC 3 cut(s) 215, 446, 641
DpnII GATC 3 cut(s) 213, 444, 639
DraIII CACNNNGTG 1 cut(s) 317
Eco130I CCWWGG 2 cut(s) 458, 624
Eco147I AGGCCT 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 102
EcoRII CCWGG 4 cut(s) 205, 265, 313, 535
EcoT14I CCWWGG 2 cut(s) 458, 624
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 2 cut(s) 458, 624
FaeI CATG 1 cut(s) 150
FaiI YATR 3 cut(s) 22, 148, 309
FaqI GGGAC 2 cut(s) 274, 506
FatI CATG 1 cut(s) 146
FbaI TGATCA 1 cut(s) 213
Fnu4HI GCNGC 8 cut(s) 6, 48, 174, 246, 501, 504, 516, 558
FokI GGATG 3 cut(s) 167, 398, 536
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 8 cut(s) 6, 48, 174, 246, 501, 504, 516, 558
FspBI CTAG 2 cut(s) 26, 459
GlaI GCGC 1 cut(s) 299
GluI GCNGC 8 cut(s) 6, 48, 174, 246, 501, 504, 516, 558
HaeIII GGCC 3 cut(s) 195, 270, 500
HapII CCGG 2 cut(s) 57, 77
HhaI GCGC 1 cut(s) 300
Hin1II CATG 1 cut(s) 150
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 1 cut(s) 393
HpaII CCGG 2 cut(s) 57, 77
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 5 cut(s) 62, 136, 320, 472, 567
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 4 cut(s) 89, 478, 587, 598
Hpy8I GTNNAC 5 cut(s) 62, 136, 320, 472, 567
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 2 cut(s) 529, 619
HpyCH4V TGCA 9 cut(s) 20, 62, 68, 146, 150, 161, 245, 311, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 512
HpyF3I CTNAG 2 cut(s) 223, 354
Hsp92II CATG 1 cut(s) 150
HspAI GCGC 1 cut(s) 298
KpnI GGTACC 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 3 cut(s) 213, 444, 639
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 6 cut(s) 34, 185, 257, 490, 502, 569
LweI GCATC 3 cut(s) 77, 148, 309
MabI ACCWGGT 1 cut(s) 313
MaeI CTAG 2 cut(s) 26, 459
MaeIII GTNAC 2 cut(s) 166, 238
MalI GATC 3 cut(s) 215, 446, 641
MboI GATC 3 cut(s) 213, 444, 639
MboII GAAGA 6 cut(s) 25, 105, 108, 175, 178, 493
MfeI CAATTG 1 cut(s) 530
MflI RGATCY 1 cut(s) 444
MhlI GDGCHC 2 cut(s) 64, 233
MluCI AATT 3 cut(s) 380, 510, 530
MnlI CCTC 4 cut(s) 281, 342, 586, 588
MroXI GAANNNNTTC 1 cut(s) 261
MseI TTAA 2 cut(s) 324, 667
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 2 cut(s) 176, 503
MspI CCGG 2 cut(s) 57, 77
MspR9I CCNGG 5 cut(s) 77, 207, 267, 315, 537
MunI CAATTG 1 cut(s) 530
MvaI CCWGG 4 cut(s) 207, 267, 315, 537
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 2 cut(s) 26, 512
NciI CCSGG 1 cut(s) 77
NdeII GATC 3 cut(s) 213, 444, 639
NlaIII CATG 1 cut(s) 150
NlaIV GGNNCC 3 cut(s) 75, 264, 622
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 150
PaeI GCATGC 1 cut(s) 150
PceI AGGCCT 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 1 cut(s) 393
PflFI GACNNNGTC 1 cut(s) 593
PkrI GCNGC 8 cut(s) 7, 49, 175, 247, 502, 505, 517, 559
Psp6I CCWGG 4 cut(s) 205, 265, 313, 535
PspGI CCWGG 4 cut(s) 205, 265, 313, 535
PspN4I GGNNCC 3 cut(s) 75, 264, 622
PspPI GGNCC 1 cut(s) 73
PstI CTGCAG 2 cut(s) 247, 559
PsuI RGATCY 1 cut(s) 444
PsyI GACNNNGTC 1 cut(s) 593
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 2 cut(s) 541, 622
RsaNI GTAC 2 cut(s) 540, 621
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 2 cut(s) 324, 667
SatI GCNGC 8 cut(s) 6, 48, 174, 246, 501, 504, 516, 558
Sau3AI GATC 3 cut(s) 213, 444, 639
Sau96I GGNCC 1 cut(s) 73
ScrFI CCNGG 5 cut(s) 77, 207, 267, 315, 537
SduI GDGCHC 2 cut(s) 64, 233
SexAI ACCWGGT 1 cut(s) 313
SfaNI GCATC 3 cut(s) 77, 148, 309
SfcI CTRYAG 2 cut(s) 243, 555
SinI GGWCC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 345
SphI GCATGC 1 cut(s) 150
Sse9I AATT 3 cut(s) 380, 510, 530
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 1 cut(s) 501
SspMI CTAG 2 cut(s) 26, 459
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 5 cut(s) 75, 205, 265, 313, 535
StyI CCWWGG 2 cut(s) 458, 624
TaqI TCGA 2 cut(s) 189, 303
TasI AATT 3 cut(s) 380, 510, 530
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 1 cut(s) 393
Tru1I TTAA 2 cut(s) 324, 667
Tru9I TTAA 2 cut(s) 324, 667
TscAI CASTG 4 cut(s) 49, 231, 285, 358
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 7 cut(s) 5, 47, 173, 245, 503, 515, 557
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 1 cut(s) 385
TspRI CASTG 4 cut(s) 49, 231, 285, 358
Tth111I GACNNNGTC 1 cut(s) 593
VneI GTGCAC 1 cut(s) 60
VpaK11BI GGWCC 1 cut(s) 73
XceI RCATGY 1 cut(s) 150
XmaJI CCTAGG 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 2 cut(s) 26, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.