Prupe.4G017300_v2.0.a1

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0020348
Physical Location & Seq
Forward (+)
811486 .. 811896
411 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G017300.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 411 bp
ATGAAATCTCTCCAAGTTTTCTTTATGATAGCCATGCTCATTATGGCTTTAGCCATTACTCTCTCTGCAACGGTACCAAACCAAAGAGAAGCATTCTTCAGGCCAGGATCGTCAACAAGCCGGTTCCTTGCCTCTCAGTCGTCTCCAGGTCGGGGTGGATGCGACCAAAACCCTCTTGCTTGTAGAGCCACTGAAGGCAGTGCAGGGCCAGATTGCTGCAGCAAAAAATGTGTGGATTTGAGGACAGACATATCCAACTGTGGTTCTTGTGGGAAGAGATGCATCAGCTCAGAGATATGCTGTAACGCTCATTGTGTGAATCCAATGTCTCACAATCAAAACTGTGGCAAGTGCTCCAACCACTGCAAGGAAGGCACTTCTTGTGACAATGGAATGTGCGACTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

137

Amino Acids

14.45

Weight (kDa)

8.15

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020348)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17590
malus_domestica MD10G1324900.v1.1
prunus_persica Prupe.4G017300_v2.0.a1 Prupe.4G020700_v2.0.a1 Prupe.4G199900_v2.0.a1 Prupe.4G239000_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 73
AclWI GGATC 1 cut(s) 115
AcuI CTGAAG 2 cut(s) 82, 213
AfaI GTAC 1 cut(s) 75
AfiI CCNNNNNNNGG 2 cut(s) 152, 367
AjnI CCWGG 2 cut(s) 103, 145
AluBI AGCT 1 cut(s) 288
AluI AGCT 1 cut(s) 288
Alw21I GWGCWC 1 cut(s) 356
Alw26I GTCTC 2 cut(s) 147, 333
AlwI GGATC 1 cut(s) 115
AoxI GGCC 2 cut(s) 101, 206
ApeKI GCWGC 2 cut(s) 216, 219
Asp718I GGTACC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 206
BanI GGYRCC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 356
BbvI GCAGC 2 cut(s) 203, 231
BciT130I CCWGG 2 cut(s) 105, 147
BcoDI GTCTC 2 cut(s) 147, 333
BfmI CTRYAG 1 cut(s) 217
BisI GCNGC 2 cut(s) 217, 220
BlsI GCNGC 2 cut(s) 218, 221
Bme1390I CCNGG 2 cut(s) 105, 147
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 75, 125
BmrFI CCNGG 2 cut(s) 105, 147
BmsI GCATC 3 cut(s) 149, 269, 291
BpmI CTGGAG 1 cut(s) 129
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 367
Bse118I RCCGGY 1 cut(s) 120
BseBI CCWGG 2 cut(s) 105, 147
BseGI GGATG 1 cut(s) 164
BseLI CCNNNNNNNGG 2 cut(s) 152, 367
BseMII CTCAG 2 cut(s) 149, 303
BseXI GCAGC 2 cut(s) 203, 231
BsgI GTGCAG 1 cut(s) 222
BshFI GGCC 2 cut(s) 103, 208
BshNI GGYRCC 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 356
BsiSI CCGG 1 cut(s) 121
BslI CCNNNNNNNGG 2 cut(s) 152, 367
BsmAI GTCTC 2 cut(s) 147, 333
BsmBI CGTCTC 1 cut(s) 147
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 2 cut(s) 103, 208
Bsp1286I GDGCHC 1 cut(s) 356
Bsp143I GATC 1 cut(s) 107
BspANI GGCC 2 cut(s) 103, 208
BspCNI CTCAG 2 cut(s) 148, 302
BspLI GGNNCC 2 cut(s) 75, 125
BspMAI CTGCAG 1 cut(s) 221
BspPI GGATC 1 cut(s) 115
BspT107I GGYRCC 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 120
BssMI GATC 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 105, 147
Bst4CI ACNGT 3 cut(s) 73, 260, 344
Bst6I CTCTTC 1 cut(s) 269
BstDEI CTNAG 2 cut(s) 135, 289
BstF5I GGATG 1 cut(s) 164
BstKTI GATC 1 cut(s) 110
BstMAI GTCTC 2 cut(s) 147, 333
BstMBI GATC 1 cut(s) 107
BstMWI GCNNNNNNNGC 2 cut(s) 185, 372
BstNI CCWGG 2 cut(s) 105, 147
BstSCI CCNGG 2 cut(s) 103, 145
BstSFI CTRYAG 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 203, 231
BsuRI GGCC 2 cut(s) 103, 208
BtsCI GGATG 1 cut(s) 164
BtsI GCAGTG 2 cut(s) 205, 361
BtsIMutI CAGTG 3 cut(s) 189, 205, 361
Cfr10I RCCGGY 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 1 cut(s) 74
CviAII CATG 1 cut(s) 34
CviJI RGCY 8 cut(s) 32, 47, 53, 103, 120, 188, 208, 288
CviKI_1 RGCY 8 cut(s) 32, 47, 53, 103, 120, 188, 208, 288
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 2 cut(s) 135, 289
DpnI GATC 1 cut(s) 109
DpnII GATC 1 cut(s) 107
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Eco57I CTGAAG 2 cut(s) 82, 213
EcoRII CCWGG 2 cut(s) 103, 145
EcoT22I ATGCAT 2 cut(s) 284, 409
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 1 cut(s) 37
FaiI YATR 7 cut(s) 26, 35, 44, 251, 298, 405, 409
FatI CATG 1 cut(s) 33
Fnu4HI GCNGC 2 cut(s) 217, 220
FokI GGATG 1 cut(s) 171
Fsp4HI GCNGC 2 cut(s) 217, 220
GluI GCNGC 2 cut(s) 217, 220
GsuI CTGGAG 1 cut(s) 129
HaeIII GGCC 2 cut(s) 103, 208
HapII CCGG 1 cut(s) 121
Hin1II CATG 1 cut(s) 37
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 1 cut(s) 319
HpaII CCGG 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 1 cut(s) 292
Hpy8I GTNNAC 1 cut(s) 114
HpyAV CCTTC 2 cut(s) 188, 365
HpyCH4III ACNGT 3 cut(s) 73, 260, 344
HpyCH4V TGCA 6 cut(s) 68, 203, 219, 282, 366, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 372
HpyF3I CTNAG 2 cut(s) 135, 289
Hsp92II CATG 1 cut(s) 37
KpnI GGTACC 1 cut(s) 77
Kzo9I GATC 1 cut(s) 107
LmnI GCTCC 1 cut(s) 359
LpnPI CCDG 8 cut(s) 85, 90, 117, 132, 134, 159, 189, 222
Lsp1109I GCAGC 2 cut(s) 203, 231
LweI GCATC 3 cut(s) 149, 269, 291
MaeIII GTNAC 2 cut(s) 302, 383
MalI GATC 1 cut(s) 109
MboI GATC 1 cut(s) 107
MboII GAAGA 2 cut(s) 88, 286
MhlI GDGCHC 1 cut(s) 356
MmeI TCCRAC 2 cut(s) 279, 381
MnlI CCTC 3 cut(s) 142, 183, 234
Mph1103I ATGCAT 2 cut(s) 284, 409
MspI CCGG 1 cut(s) 121
MspR9I CCNGG 2 cut(s) 105, 147
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 2 cut(s) 105, 147
MwoI GCNNNNNNNGC 2 cut(s) 185, 372
NdeII GATC 1 cut(s) 107
NlaIII CATG 1 cut(s) 37
NlaIV GGNNCC 2 cut(s) 75, 125
NmuCI GTSAC 1 cut(s) 383
NsiI ATGCAT 2 cut(s) 284, 409
PctI GAATGC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 319
PkrI GCNGC 2 cut(s) 218, 221
Psp6I CCWGG 2 cut(s) 103, 145
PspGI CCWGG 2 cut(s) 103, 145
PspN4I GGNNCC 2 cut(s) 75, 125
PspPI GGNCC 1 cut(s) 206
PstI CTGCAG 1 cut(s) 221
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
SatI GCNGC 2 cut(s) 217, 220
Sau3AI GATC 1 cut(s) 107
Sau96I GGNCC 1 cut(s) 206
ScrFI CCNGG 2 cut(s) 105, 147
SduI GDGCHC 1 cut(s) 356
SetI ASST 2 cut(s) 151, 290
SfaNI GCATC 3 cut(s) 149, 269, 291
SfcI CTRYAG 1 cut(s) 217
StyD4I CCNGG 2 cut(s) 103, 145
TaaI ACNGT 3 cut(s) 73, 260, 344
TfiI GAWTC 1 cut(s) 319
TscAI CASTG 3 cut(s) 196, 205, 368
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 2 cut(s) 216, 219
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 196, 205, 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
Zsp2I ATGCAT 2 cut(s) 284, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.