Prupe.4G030100_v2.0.a1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0015568
Physical Location & Seq
Forward (+)
1402972 .. 1406168
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G030100.4

Sequence Viewer

Length: 1620 bp
ATGCAAGTTTGCTGTGATCTTCAAGTCGATGTCAATGGTGAAGAGGTTTTCATGGTGGACAAGAAAACGTTGGCGTCTTTCTCCGGTAGATTTAGCAAATTATTTGGTAAACTGAAGGGTACATCGAGGAGCCTAAAAGTGATATTCCATGACTTTCCTGGAGGTGCAGAGGGTTTTGAGCTCATGACAAGGTTTTGTTACAATAATGGCACAACTGAGATAACCCCTTCCAACATAGTCTTACTATACTGCATCGCGCATTTCATGGAGATGGATGGTGATGGCTCTGGAAGACTCAACATACTATCCCAAACAGAGAAATCTCTTGAAGGGATCAGCTTTTGGACATGGCCTAACCTTGTAGTAGCTCTAAAGCAATGCCAAGATTTACTTCCTGCCTCAAATTGTTCATCAATATTGGAGAAAGTCCTGGACTGCCTCATAGCCAAGCTTTTTTGCCCATTTGTTAGAAGCACATATGCGTCTTCATCAGAACATTTGAGTTTCCAGTTTTCAAGTGCAAGGAGCAGTCATAGTATGAAAACCAATTGCTCTCAAACAACATGGTGGGTCGAAGACCTTATGTTCTTGAGTATCAATTTAATTGAGAAGGTGATAAGAAGGATGATGTTCCAGGAGCTTTATCATTCTACAATTTTCAAGTTTCTATTCCATTATCATCAATCAAAGTCTATGGGTGCCACCACACCAGGTGAGAAGCGCAAAATTACAGAGGTTGTTATTAGTCTGCTTTATTTGCTTGATAGAAGCTCCCTTTCTTGCAAACGCTTATTCAAGATATACCAACTGGCTTTGAGCCTGAAAATAAGCAAACTATACAAAAACAAGTTAGAGAATATGATAGGCTCACAGCTAGATCAAGCAACGATTGATTATGTACTTGTTCCATCTCCACGCGGGAAGAAGTACGTATATGATGTGAGTTTGGTTTTAAGATTTGTGAAATCATTTCTCTTTGAAAAGGGATGCCATTTATCTCAGGGCCGCTTAAAAAAGGTCTCCAAGTTGATGGATTCATACCTTGCAGAAGTGGCACCAGATACCTATTTGAATCCTTCAAAGTTTGCAGCATTGGCGATGTCACTGCCAGATTCTGCACGAGAATCTCATGACAGACTTTACCAAGCAATTGGTGTATATTTTAAGCGTCACGCTGATTTATTCGAAGAAGAGAAGATGAGCATCTGTTGTGCACTGAACTATGATAAACTATCAGCACATTCTTTGAAACATCTCACTCAAAATATAAAGTTTCCTCCAAGAAGAGCAGTTGAAGCTTTTACTACAGAGCAATCCAAACTGAGAAGTTTACTCCACAAAGCCTACAATCTCAAGACTTTAGACTCTCTGCTTATTCACACACAGAATGAAATTAAGCATGAGAAACAGGACACTGAGCATATCATCCCTATCTATACTAAGAAGCTTGATCTCCCAACAGAGGCAGAGAAGCTAAGAGCAGATTTGCAAACCATGCATTGGAAGACATTGGAATTGGGAAACACTTGTGGGACAATGGAGATGCAGATGGCAGCTATAACGAAATCAAGATTGTCCTTTAGGGGTAGTATTCAATACTTGCCTAAACTCTTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

540

Amino Acids

61.67

Weight (kDa)

9.05

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015568)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03040
malus_domestica MD05G1335300.v1.1
prunus_persica Prupe.4G030100_v2.0.a1 Prupe.4G030100_v2.0.a1 Prupe.4G030100_v2.0.a1 Prupe.4G030100_v2.0.a1
pyrus_communis pycom05g30670
rosa_chinensis RchiOBHm_Chr5g0004691
rosa_laevigata RLG00000031252
rosa_roxburghii Rroxscaffold_1G00070870
rosa_rugosa Rorug04G0413300
rosa_samantha Rh5AG040900 Rh5BG040600 Rh5CG044400 Rh5DG040600
rosa_wichuraiana Rw5G003940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 698, 1054
AccB7I CCANNNNNTGG 1 cut(s) 1500
AccII CGCG 2 cut(s) 257, 918
AciI CCGC 2 cut(s) 918, 1006
AclI AACGTT 1 cut(s) 68
AclWI GGATC 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 134
AcyI GRCGYC 1 cut(s) 74
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 3 cut(s) 121, 900, 929
AfiI CCNNNNNNNGG 2 cut(s) 1462, 1500
AjnI CCWGG 4 cut(s) 157, 429, 633, 709
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
Alw21I GWGCWC 2 cut(s) 183, 1216
Alw26I GTCTC 1 cut(s) 1024
Alw44I GTGCAC 1 cut(s) 1212
AlwI GGATC 1 cut(s) 341
AlwNI CAGNNNCTG 1 cut(s) 1115
AoxI GGCC 2 cut(s) 350, 1003
ApaLI GTGCAC 1 cut(s) 1212
ApeKI GCWGC 2 cut(s) 1088, 1553
AspLEI GCGC 2 cut(s) 259, 723
AspS9I GGNCC 1 cut(s) 1003
AsuHPI GGTGA 4 cut(s) 50, 290, 625, 725
AsuII TTCGAA 1 cut(s) 1185
BaeGI GKGCMC 1 cut(s) 1216
BanI GGYRCC 2 cut(s) 698, 1054
BanII GRGCYC 1 cut(s) 183
BauI CACGAG 1 cut(s) 1119
BbsI GAAGAC 4 cut(s) 298, 477, 582, 1511
Bbv12I GWGCWC 2 cut(s) 183, 1216
BbvI GCAGC 2 cut(s) 1100, 1565
BccI CCATC 6 cut(s) 265, 269, 275, 916, 1024, 1543
BcgI CGANNNNNNTGC 2 cut(s) 1087, 1121
BciT130I CCWGG 4 cut(s) 159, 431, 635, 711
BcoDI GTCTC 1 cut(s) 1024
BfaI CTAG 1 cut(s) 875
BfmI CTRYAG 1 cut(s) 1305
BisI GCNGC 3 cut(s) 1006, 1089, 1554
BlsI GCNGC 3 cut(s) 1007, 1090, 1555
Bme1390I CCNGG 4 cut(s) 159, 431, 635, 711
BmgT120I GGNCC 1 cut(s) 1003
BmiI GGNNCC 3 cut(s) 131, 700, 1056
BmrFI CCNGG 4 cut(s) 159, 431, 635, 711
BmsI GCATC 4 cut(s) 261, 977, 1212, 1533
BpiI GAAGAC 4 cut(s) 298, 477, 582, 1511
BpmI CTGGAG 1 cut(s) 180
Bpu14I TTCGAA 1 cut(s) 1185
BpuEI CTTGAG 2 cut(s) 610, 1337
BsaAI YACGTR 1 cut(s) 931
BsaBI GATNNNNATC 1 cut(s) 1202
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 1024
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 2 cut(s) 1462, 1500
Bse1I ACTGG 2 cut(s) 508, 813
Bse3DI GCAATG 1 cut(s) 383
Bse8I GATNNNNATC 1 cut(s) 1202
BseBI CCWGG 4 cut(s) 159, 431, 635, 711
BseGI GGATG 4 cut(s) 280, 630, 992, 1425
BseJI GATNNNNATC 1 cut(s) 1202
BseLI CCNNNNNNNGG 2 cut(s) 1462, 1500
BseMI GCAATG 1 cut(s) 383
BseMII CTCAG 4 cut(s) 207, 1013, 1313, 1407
BseNI ACTGG 2 cut(s) 508, 813
BseRI GAGGAG 1 cut(s) 142
BseSI GKGCMC 1 cut(s) 1216
BseXI GCAGC 2 cut(s) 1100, 1565
BsgI GTGCAG 2 cut(s) 186, 1101
Bsh1236I CGCG 2 cut(s) 257, 918
BshFI GGCC 2 cut(s) 352, 1005
BshNI GGYRCC 2 cut(s) 698, 1054
BsiHKAI GWGCWC 2 cut(s) 183, 1216
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 1546
BslI CCNNNNNNNGG 2 cut(s) 1462, 1500
BsmAI GTCTC 1 cut(s) 1024
BsmFI GGGAC 1 cut(s) 1546
BsnI GGCC 2 cut(s) 352, 1005
Bso31I GGTCTC 1 cut(s) 1024
Bsp119I TTCGAA 1 cut(s) 1185
Bsp1286I GDGCHC 2 cut(s) 183, 1216
Bsp143I GATC 4 cut(s) 16, 333, 877, 1450
BspACI CCGC 2 cut(s) 918, 1006
BspANI GGCC 2 cut(s) 352, 1005
BspCNI CTCAG 4 cut(s) 208, 1012, 1314, 1408
BspFNI CGCG 2 cut(s) 257, 918
BspHI TCATGA 2 cut(s) 183, 1129
BspLI GGNNCC 3 cut(s) 131, 700, 1056
BspPI GGATC 1 cut(s) 341
BspQI GCTCTTC 1 cut(s) 1279
BspT104I TTCGAA 1 cut(s) 1185
BspT107I GGYRCC 2 cut(s) 698, 1054
BspTNI GGTCTC 1 cut(s) 1024
BsrDI GCAATG 1 cut(s) 383
BsrI ACTGG 2 cut(s) 508, 813
BssMI GATC 4 cut(s) 16, 333, 877, 1450
BssNI GRCGYC 1 cut(s) 74
BssSI CACGAG 1 cut(s) 1119
Bst2BI CACGAG 1 cut(s) 1119
Bst2UI CCWGG 4 cut(s) 159, 431, 635, 711
Bst6I CTCTTC 3 cut(s) 36, 1185, 1279
BstACI GRCGYC 1 cut(s) 74
BstAPI GCANNNNNTGC 1 cut(s) 1495
BstBAI YACGTR 1 cut(s) 931
BstBI TTCGAA 1 cut(s) 1185
BstDEI CTNAG 6 cut(s) 216, 999, 1322, 1416, 1440, 1475
BstF5I GGATG 4 cut(s) 280, 630, 992, 1425
BstFNI CGCG 2 cut(s) 257, 918
BstHHI GCGC 2 cut(s) 259, 723
BstKTI GATC 4 cut(s) 19, 336, 880, 1453
BstMAI GTCTC 1 cut(s) 1024
BstMBI GATC 4 cut(s) 16, 333, 877, 1450
BstMWI GCNNNNNNNGC 5 cut(s) 757, 1052, 1094, 1295, 1495
BstNI CCWGG 4 cut(s) 159, 431, 635, 711
BstSCI CCNGG 4 cut(s) 157, 429, 633, 709
BstSFI CTRYAG 1 cut(s) 1305
BstSLI GKGCMC 1 cut(s) 1216
BstSNI TACGTA 1 cut(s) 931
BstUI CGCG 2 cut(s) 257, 918
BstV1I GCAGC 2 cut(s) 1100, 1565
BstV2I GAAGAC 4 cut(s) 298, 477, 582, 1511
BstXI CCANNNNNNTGG 2 cut(s) 1030, 1151
BsuRI GGCC 2 cut(s) 352, 1005
BtgZI GCGATG 2 cut(s) 238, 1112
BtsCI GGATG 4 cut(s) 280, 630, 992, 1425
BtsI GCAGTG 1 cut(s) 1103
BtsIMutI CAGTG 3 cut(s) 1103, 1214, 1413
CaiI CAGNNNCTG 1 cut(s) 1115
CciI TCATGA 2 cut(s) 183, 1129
CfoI GCGC 2 cut(s) 259, 723
Cfr13I GGNCC 1 cut(s) 1003
CseI GACGC 3 cut(s) 63, 471, 1157
CsiI ACCWGGT 1 cut(s) 709
Csp6I GTAC 3 cut(s) 120, 899, 928
CviAII CATG 9 cut(s) 52, 149, 184, 265, 348, 564, 1130, 1400, 1495
CviQI GTAC 3 cut(s) 120, 899, 928
DdeI CTNAG 6 cut(s) 216, 999, 1322, 1416, 1440, 1475
DpnI GATC 4 cut(s) 18, 335, 879, 1452
DpnII GATC 4 cut(s) 16, 333, 877, 1450
DraIII CACNNNGTG 1 cut(s) 713
Eam1104I CTCTTC 3 cut(s) 36, 1185, 1279
EarI CTCTTC 3 cut(s) 36, 1185, 1279
Ecl136II GAGCTC 1 cut(s) 181
Eco105I TACGTA 1 cut(s) 931
Eco24I GRGCYC 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 1024
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 181
EcoRII CCWGG 4 cut(s) 157, 429, 633, 709
EcoT22I ATGCAT 1 cut(s) 1500
EcoT38I GRGCYC 1 cut(s) 183
FaeI CATG 9 cut(s) 55, 152, 187, 268, 351, 567, 1133, 1403, 1498
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FaqI GGGAC 1 cut(s) 1546
FatI CATG 9 cut(s) 51, 148, 183, 264, 347, 563, 1129, 1399, 1494
FauI CCCGC 1 cut(s) 911
FauNDI CATATG 1 cut(s) 478
Fnu4HI GCNGC 3 cut(s) 1006, 1089, 1554
FokI GGATG 4 cut(s) 287, 637, 999, 1412
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 3 cut(s) 1006, 1089, 1554
FspBI CTAG 1 cut(s) 875
GlaI GCGC 2 cut(s) 258, 722
GluI GCNGC 3 cut(s) 1006, 1089, 1554
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 2 cut(s) 352, 1005
HapII CCGG 1 cut(s) 84
HgaI GACGC 3 cut(s) 63, 471, 1157
HhaI GCGC 2 cut(s) 259, 723
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 9 cut(s) 55, 152, 187, 268, 351, 567, 1133, 1403, 1498
Hin6I GCGC 2 cut(s) 257, 721
HinP1I GCGC 2 cut(s) 257, 721
HindIII AAGCTT 3 cut(s) 449, 1296, 1445
HinfI GANTC 6 cut(s) 294, 1034, 1072, 1112, 1124, 1364
HpaII CCGG 1 cut(s) 84
HphI GGTGA 4 cut(s) 50, 290, 625, 725
Hpy166II GTNNAC 4 cut(s) 58, 110, 1214, 1331
Hpy188I TCNGA 1 cut(s) 493
Hpy188III TCNNGA 8 cut(s) 184, 288, 326, 589, 796, 1130, 1354, 1569
Hpy8I GTNNAC 4 cut(s) 58, 110, 1214, 1331
HpyAV CCTTC 6 cut(s) 109, 237, 323, 604, 615, 1086
HpyCH4IV ACGT 2 cut(s) 68, 930
HpyF10VI GCNNNNNNNGC 5 cut(s) 757, 1052, 1094, 1295, 1495
HpyF3I CTNAG 6 cut(s) 216, 999, 1322, 1416, 1440, 1475
HpySE526I ACGT 2 cut(s) 68, 930
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 9 cut(s) 55, 152, 187, 268, 351, 567, 1133, 1403, 1498
HspAI GCGC 2 cut(s) 257, 721
Kzo9I GATC 4 cut(s) 16, 333, 877, 1450
LguI GCTCTTC 1 cut(s) 1279
LmnI GCTCC 4 cut(s) 129, 525, 637, 776
Lsp1109I GCAGC 2 cut(s) 1100, 1565
LweI GCATC 4 cut(s) 261, 977, 1212, 1533
MabI ACCWGGT 1 cut(s) 709
MaeI CTAG 1 cut(s) 875
MaeII ACGT 2 cut(s) 68, 930
MaeIII GTNAC 3 cut(s) 197, 1101, 1169
MalI GATC 4 cut(s) 18, 335, 879, 1452
MboI GATC 4 cut(s) 16, 333, 877, 1450
MfeI CAATTG 2 cut(s) 547, 1149
MhlI GDGCHC 2 cut(s) 183, 1216
MlyI GAGTC 2 cut(s) 288, 1358
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 9 cut(s) 37, 120, 155, 163, 409, 449, 727, 1287, 1456
Mph1103I ATGCAT 1 cut(s) 1500
MseI TTAA 5 cut(s) 602, 953, 1010, 1164, 1395
MslI CAYNNNNRTG 1 cut(s) 269
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 4 cut(s) 159, 431, 635, 711
MunI CAATTG 2 cut(s) 547, 1149
MvaI CCWGG 4 cut(s) 159, 431, 635, 711
MvnI CGCG 2 cut(s) 257, 918
MwoI GCNNNNNNNGC 5 cut(s) 757, 1052, 1094, 1295, 1495
NdeI CATATG 1 cut(s) 478
NdeII GATC 4 cut(s) 16, 333, 877, 1450
NlaIII CATG 9 cut(s) 55, 152, 187, 268, 351, 567, 1133, 1403, 1498
NlaIV GGNNCC 3 cut(s) 131, 700, 1056
NmuCI GTSAC 2 cut(s) 1101, 1169
NsiI ATGCAT 1 cut(s) 1500
NspV TTCGAA 1 cut(s) 1185
PagI TCATGA 2 cut(s) 183, 1129
PciSI GCTCTTC 1 cut(s) 1279
PfeI GAWTC 4 cut(s) 1034, 1072, 1112, 1124
PflMI CCANNNNNTGG 1 cut(s) 1500
PfoI TCCNGGA 3 cut(s) 157, 429, 633
PkrI GCNGC 3 cut(s) 1007, 1090, 1555
PleI GAGTC 2 cut(s) 288, 1358
PpsI GAGTC 2 cut(s) 288, 1358
Ppu21I YACGTR 1 cut(s) 931
Psp124BI GAGCTC 1 cut(s) 183
Psp1406I AACGTT 1 cut(s) 68
Psp6I CCWGG 4 cut(s) 157, 429, 633, 709
PspGI CCWGG 4 cut(s) 157, 429, 633, 709
PspN4I GGNNCC 3 cut(s) 131, 700, 1056
PspPI GGNCC 1 cut(s) 1003
PstNI CAGNNNCTG 1 cut(s) 1115
RsaI GTAC 3 cut(s) 121, 900, 929
RsaNI GTAC 3 cut(s) 120, 899, 928
RseI CAYNNNNRTG 1 cut(s) 269
SacI GAGCTC 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 1279
SaqAI TTAA 5 cut(s) 602, 953, 1010, 1164, 1395
SatI GCNGC 3 cut(s) 1006, 1089, 1554
Sau3AI GATC 4 cut(s) 16, 333, 877, 1450
Sau96I GGNCC 1 cut(s) 1003
SchI GAGTC 2 cut(s) 288, 1358
ScrFI CCNGG 4 cut(s) 159, 431, 635, 711
SduI GDGCHC 2 cut(s) 183, 1216
SexAI ACCWGGT 1 cut(s) 709
SfaNI GCATC 4 cut(s) 261, 977, 1212, 1533
SfcI CTRYAG 1 cut(s) 1305
SfuI TTCGAA 1 cut(s) 1185
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 2 cut(s) 589, 1352
SmoI CTYRAG 2 cut(s) 589, 1352
SnaBI TACGTA 1 cut(s) 931
SsiI CCGC 2 cut(s) 918, 1006
SspI AATATT 1 cut(s) 417
SspMI CTAG 1 cut(s) 875
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 4 cut(s) 157, 429, 633, 709
TaiI ACGT 2 cut(s) 71, 933
TaqI TCGA 4 cut(s) 27, 125, 573, 1185
TatI WGTACW 1 cut(s) 898
TauI GCSGC 1 cut(s) 1008
TfiI GAWTC 4 cut(s) 1034, 1072, 1112, 1124
Tru1I TTAA 5 cut(s) 602, 953, 1010, 1164, 1395
Tru9I TTAA 5 cut(s) 602, 953, 1010, 1164, 1395
TscAI CASTG 3 cut(s) 1110, 1221, 1420
TseFI GTSAC 2 cut(s) 1101, 1169
TseI GCWGC 2 cut(s) 1088, 1553
Tsp45I GTSAC 2 cut(s) 1101, 1169
TspDTI ATGAA 7 cut(s) 40, 253, 399, 477, 554, 1026, 1404
TspRI CASTG 3 cut(s) 1110, 1221, 1420
Van91I CCANNNNNTGG 1 cut(s) 1500
VneI GTGCAC 1 cut(s) 1212
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
XspI CTAG 1 cut(s) 875
Zsp2I ATGCAT 1 cut(s) 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.