RLG00000031252

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015568
Physical Location & Seq
Forward (+)
3402617 .. 3404926
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031252

Sequence Viewer

Length: 1569 bp
ATGGCAATGTTCTGTGATCTCCAAGTTGATGTCAATGGCGAAGAAGTCTTCATGGTGAACAAGAAAACTTTGGCATCTTTCTCTTGTCGATTAAGCAGACTATTTGGTAAATCAATGGGTACAACGAGCCTCAAAGTGATATTCCATGACTTTCCTGGGGGTGCAGAGGGTTTTGAGCTCATGACAAGGTTTTGTTACAACAATGGAACAACCGAAATAACTCCTTCCAACTTAGTCCTTGTATACTGCATCGCAGATTTCATGGAGATGGATGGTGATATACTATTGCAAGCTCAAAACACTCTTGATGGTATCAGCTCTTGGACGTGGTCTGAGCTTCTAGTAGCTCTGAAGCAGTGCCAAGATTTACTTGCCCCCTCAAATTCTTCATTGATATTGCAGATAGTCATGGACTGCATCATAGGCAAGCTTTCTTGTCCATTTTTTCCAACCCCCAATGACACTACTTCATCAGAGAACGTGAGTAGTTTCCAGTTTTCTAGTGGTTCGAGGAGCAGTTATAATCTGAAAAGCAATTGCTCTATGAAAACATGGTGGTTTGAAGACTTGATGTTCTTAAATACCAAATCGATCGAAATGGTTATAAGAAGCCTGATATCCAAGGAGATTGAACAGTCTACAGTTTTCAAGTTTCTCCTTCGTTATCATCAGTCAAAGTGTTTTGGTGCCAAACCAGAAGAGAAGTACAAGATCACAGAGGTTGTTATCGGTCTACTTTCTCTGCTTGATAGGAGCTCCCTTTCTTTCAAGGGATTATTCAATATATATCAAGCGGCTTTGAACATGAAAGTAGGCAAGAAATACAAAAACAAGTTGGAGATTATGATAGGTTCACAGCTGGATCAAGCAACAATCAATTATTTACTTGTTCCATCTCCCTGTGGGAAGAAGTATGTTTATGATGTGAATTTGATTTTGAGGTTAGGGAAATCATTTCTTCTTCAAGAAGGGGGTCATTTATCCCAGAAGAGTCACTTCACAAAGGTTGCCAAGTTGATGGATTTATACCTTGCAGAAGTGGCTCCAGATTTTCATTTGAAACCTTCAAAGTTTGCAGCATTAGCTATGATGTTGCCAGCGTCTGTGCGAGAATCTCATGACAGACTTTACGAGGCCATTGCTGTATATTTTAAGTGCCACACTGGTCTATCTGAACAAGAGAAGCTGACAATCTGTTGTGCACTGAACTATAAGAAGCTCTCAGCAGGACCTTTGAAACAAGCCTCTCGCACAAAATTCCCTTCCAGAAGAGCAGTTGAAACTTTCAAGAAACAGCGATCCAAGCTCAGAAGCGTACACCAGGAAGTCTACTACATCCGAACCAAGGATAAGAAAGAGGAAATTGAGTCAGTCCTACTTGACGCAAAGAGTCTTGATCTCCCAACAAAGGCTCAGAAGCTCAGAGCAGATTTGCAAAGGATGCATTCGAAGGCATTGGAATTGGGGAAGGTTTTCGGGACAATGCAGATACAAGTGACAAGTGTAAACAAATCAAGATTGCCCTTTCAGGCAACCAATACACGATACGTGCCAAAACTCTTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

523

Amino Acids

59.12

Weight (kDa)

9.24

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 184 - 409 1.7e-54 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015568)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03040
malus_domestica MD05G1335300.v1.1
prunus_persica Prupe.4G030100_v2.0.a1 Prupe.4G030100_v2.0.a1 Prupe.4G030100_v2.0.a1 Prupe.4G030100_v2.0.a1
pyrus_communis pycom05g30670
rosa_chinensis RchiOBHm_Chr5g0004691
rosa_laevigata RLG00000031252
rosa_roxburghii Rroxscaffold_1G00070870
rosa_rugosa Rorug04G0413300
rosa_samantha Rh5AG040900 Rh5BG040600 Rh5CG044400 Rh5DG040600
rosa_wichuraiana Rw5G003940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 522, 605
AccB1I GGYRCC 1 cut(s) 686
AccI GTMKAC 4 cut(s) 243, 638, 733, 1329
AciI CCGC 1 cut(s) 794
AclWI GGATC 2 cut(s) 870, 1293
AcsI RAATTY 3 cut(s) 382, 928, 1256
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 3 cut(s) 121, 707, 1317
AfiI CCNNNNNNNGG 2 cut(s) 1345, 1408
AjiI CACGTC 1 cut(s) 327
AjnI CCWGG 2 cut(s) 154, 1320
Alw21I GWGCWC 3 cut(s) 180, 758, 1204
Alw44I GTGCAC 1 cut(s) 1200
AlwI GGATC 2 cut(s) 870, 1293
AlwNI CAGNNNCTG 1 cut(s) 1103
AoxI GGCC 1 cut(s) 1134
ApaLI GTGCAC 1 cut(s) 1200
ApeKI GCWGC 1 cut(s) 1076
ApoI RAATTY 3 cut(s) 382, 928, 1256
Asp700I GAANNNNTTC 1 cut(s) 1472
AspS9I GGNCC 1 cut(s) 1229
AsuHPI GGTGA 2 cut(s) 67, 287
AsuII TTCGAA 1 cut(s) 1448
AvaII GGWCC 1 cut(s) 1229
BaeGI GKGCMC 1 cut(s) 1204
BanI GGYRCC 1 cut(s) 686
BanII GRGCYC 2 cut(s) 180, 758
BbsI GAAGAC 2 cut(s) 40, 570
Bbv12I GWGCWC 3 cut(s) 180, 758, 1204
BbvI GCAGC 1 cut(s) 1088
BccI CCATC 5 cut(s) 262, 266, 302, 901, 1012
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciT130I CCWGG 2 cut(s) 156, 1322
BfaI CTAG 2 cut(s) 341, 501
BfmI CTRYAG 1 cut(s) 639
BisI GCNGC 2 cut(s) 795, 1077
BlsI GCNGC 2 cut(s) 796, 1078
Bme1390I CCNGG 2 cut(s) 156, 1322
Bme18I GGWCC 1 cut(s) 1229
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 1 cut(s) 1229
BmiI GGNNCC 2 cut(s) 688, 1044
BmrFI CCNGG 2 cut(s) 156, 1322
BmsI GCATC 4 cut(s) 83, 258, 426, 1431
BpiI GAAGAC 2 cut(s) 40, 570
BpmI CTGGAG 1 cut(s) 1029
Bpu14I TTCGAA 1 cut(s) 1448
Bsa29I ATCGAT 1 cut(s) 590
BsaAI YACGTR 1 cut(s) 1549
BsaJI CCNNGG 3 cut(s) 155, 621, 1344
Bsc4I CCNNNNNNNGG 2 cut(s) 1345, 1408
Bse1I ACTGG 2 cut(s) 493, 1168
Bse3DI GCAATG 2 cut(s) 12, 1137
BseBI CCWGG 2 cut(s) 156, 1322
BseCI ATCGAT 1 cut(s) 590
BseDI CCNNGG 3 cut(s) 155, 621, 1344
BseGI GGATG 3 cut(s) 277, 1335, 1446
BseLI CCNNNNNNNGG 2 cut(s) 1345, 1408
BseMI GCAATG 2 cut(s) 12, 1137
BseMII CTCAG 5 cut(s) 324, 1236, 1321, 1427, 1435
BseNI ACTGG 2 cut(s) 493, 1168
BseRI GAGGAG 1 cut(s) 526
BseSI GKGCMC 1 cut(s) 1204
BseXI GCAGC 1 cut(s) 1088
BsgI GTGCAG 1 cut(s) 183
Bsh1285I CGRYCG 1 cut(s) 594
BshFI GGCC 1 cut(s) 1136
BshNI GGYRCC 1 cut(s) 686
BshVI ATCGAT 1 cut(s) 590
BsiEI CGRYCG 1 cut(s) 594
BsiHKAI GWGCWC 3 cut(s) 180, 758, 1204
BslFI GGGAC 1 cut(s) 1492
BslI CCNNNNNNNGG 2 cut(s) 1345, 1408
BsmFI GGGAC 1 cut(s) 1492
BsmI GAATGC 1 cut(s) 1444
BsnI GGCC 1 cut(s) 1136
Bsp119I TTCGAA 1 cut(s) 1448
Bsp1286I GDGCHC 3 cut(s) 180, 758, 1204
Bsp143I GATC 6 cut(s) 16, 591, 711, 862, 1298, 1396
BspACI CCGC 1 cut(s) 794
BspANI GGCC 1 cut(s) 1136
BspCNI CTCAG 5 cut(s) 325, 1235, 1320, 1426, 1434
BspDI ATCGAT 1 cut(s) 590
BspHI TCATGA 2 cut(s) 180, 1117
BspLI GGNNCC 2 cut(s) 688, 1044
BspPI GGATC 2 cut(s) 870, 1293
BspQI GCTCTTC 1 cut(s) 1264
BspT104I TTCGAA 1 cut(s) 1448
BspT107I GGYRCC 1 cut(s) 686
BsrDI GCAATG 2 cut(s) 12, 1137
BsrI ACTGG 2 cut(s) 493, 1168
BssECI CCNNGG 3 cut(s) 155, 621, 1344
BssMI GATC 6 cut(s) 16, 591, 711, 862, 1298, 1396
BssNAI GTATAC 1 cut(s) 244
BssT1I CCWWGG 2 cut(s) 621, 1344
Bst1107I GTATAC 1 cut(s) 244
Bst2UI CCWGG 2 cut(s) 156, 1322
Bst4CI ACNGT 2 cut(s) 636, 643
Bst6I CTCTTC 3 cut(s) 693, 983, 1264
BstAPI GCANNNNNTGC 1 cut(s) 1441
BstBAI YACGTR 1 cut(s) 1549
BstBI TTCGAA 1 cut(s) 1448
BstC8I GCNNGC 3 cut(s) 291, 428, 1098
BstDEI CTNAG 6 cut(s) 232, 333, 1222, 1307, 1413, 1421
BstF5I GGATG 3 cut(s) 277, 1335, 1446
BstKTI GATC 6 cut(s) 19, 594, 714, 865, 1301, 1399
BstMBI GATC 6 cut(s) 16, 591, 711, 862, 1298, 1396
BstMCI CGRYCG 1 cut(s) 594
BstMWI GCNNNNNNNGC 5 cut(s) 423, 1040, 1082, 1303, 1441
BstNI CCWGG 2 cut(s) 156, 1322
BstSCI CCNGG 2 cut(s) 154, 1320
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 1204
BstV1I GCAGC 1 cut(s) 1088
BstV2I GAAGAC 2 cut(s) 40, 570
BstXI CCANNNNNNTGG 1 cut(s) 1018
BstZ17I GTATAC 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 590
BsuRI GGCC 1 cut(s) 1136
BsuTUI ATCGAT 1 cut(s) 590
BtgZI GCGATG 1 cut(s) 235
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 3 cut(s) 277, 1335, 1446
BtsI GCAGTG 1 cut(s) 362
BtsIMutI CAGTG 3 cut(s) 362, 1161, 1202
Cac8I GCNNGC 3 cut(s) 291, 428, 1098
CaiI CAGNNNCTG 1 cut(s) 1103
CciI TCATGA 2 cut(s) 180, 1117
Cfr13I GGNCC 1 cut(s) 1229
ClaI ATCGAT 1 cut(s) 590
CseI GACGC 2 cut(s) 1089, 1391
Csp6I GTAC 3 cut(s) 120, 706, 1316
CviAII CATG 8 cut(s) 52, 146, 181, 262, 409, 552, 805, 1118
CviQI GTAC 3 cut(s) 120, 706, 1316
DdeI CTNAG 6 cut(s) 232, 333, 1222, 1307, 1413, 1421
DpnI GATC 6 cut(s) 18, 593, 713, 864, 1300, 1398
DpnII GATC 6 cut(s) 16, 591, 711, 862, 1298, 1396
Eam1104I CTCTTC 3 cut(s) 693, 983, 1264
EarI CTCTTC 3 cut(s) 693, 983, 1264
Ecl136II GAGCTC 2 cut(s) 178, 756
Eco130I CCWWGG 2 cut(s) 621, 1344
Eco24I GRGCYC 2 cut(s) 180, 758
Eco32I GATATC 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 1229
Eco53kI GAGCTC 2 cut(s) 178, 756
Eco57I CTGAAG 1 cut(s) 371
EcoICRI GAGCTC 2 cut(s) 178, 756
EcoO109I RGGNCCY 1 cut(s) 1229
EcoRII CCWGG 2 cut(s) 154, 1320
EcoRV GATATC 1 cut(s) 618
EcoT14I CCWWGG 2 cut(s) 621, 1344
EcoT22I ATGCAT 1 cut(s) 1446
EcoT38I GRGCYC 2 cut(s) 180, 758
ErhI CCWWGG 2 cut(s) 621, 1344
FaeI CATG 8 cut(s) 55, 149, 184, 265, 412, 555, 808, 1121
FalI AAGNNNNNCTT 4 cut(s) 354, 386, 980, 1012
FaqI GGGAC 1 cut(s) 1492
FatI CATG 8 cut(s) 51, 145, 180, 261, 408, 551, 804, 1117
FblI GTMKAC 4 cut(s) 243, 638, 733, 1329
Fnu4HI GCNGC 2 cut(s) 795, 1077
FokI GGATG 3 cut(s) 284, 1322, 1453
FriOI GRGCYC 2 cut(s) 180, 758
Fsp4HI GCNGC 2 cut(s) 795, 1077
FspBI CTAG 2 cut(s) 341, 501
GluI GCNGC 2 cut(s) 795, 1077
GsuI CTGGAG 1 cut(s) 1029
HaeIII GGCC 1 cut(s) 1136
HgaI GACGC 2 cut(s) 1089, 1391
Hin1II CATG 8 cut(s) 55, 149, 184, 265, 412, 555, 808, 1121
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 4 cut(s) 991, 1112, 1367, 1390
HphI GGTGA 2 cut(s) 67, 287
Hpy166II GTNNAC 9 cut(s) 58, 244, 639, 734, 854, 1202, 1318, 1330, 1507
Hpy188I TCNGA 9 cut(s) 334, 351, 475, 528, 1174, 1310, 1340, 1416, 1424
Hpy8I GTNNAC 9 cut(s) 58, 244, 639, 734, 854, 1202, 1318, 1330, 1507
HpyAV CCTTC 7 cut(s) 234, 668, 962, 1074, 1272, 1444, 1462
HpyCH4III ACNGT 2 cut(s) 636, 643
HpyCH4IV ACGT 3 cut(s) 326, 480, 1548
HpyF10VI GCNNNNNNNGC 5 cut(s) 423, 1040, 1082, 1303, 1441
HpyF3I CTNAG 6 cut(s) 232, 333, 1222, 1307, 1413, 1421
HpySE526I ACGT 3 cut(s) 326, 480, 1548
Hsp92II CATG 8 cut(s) 55, 149, 184, 265, 412, 555, 808, 1121
Kzo9I GATC 6 cut(s) 16, 591, 711, 862, 1298, 1396
LguI GCTCTTC 1 cut(s) 1264
LmnI GCTCC 4 cut(s) 513, 753, 761, 1048
Lsp1109I GCAGC 1 cut(s) 1088
LweI GCATC 4 cut(s) 83, 258, 426, 1431
MaeI CTAG 2 cut(s) 341, 501
MaeII ACGT 3 cut(s) 326, 480, 1548
MaeIII GTNAC 3 cut(s) 194, 992, 1495
MalI GATC 6 cut(s) 18, 593, 713, 864, 1300, 1398
MboI GATC 6 cut(s) 16, 591, 711, 862, 1298, 1396
MfeI CAATTG 1 cut(s) 535
MhlI GDGCHC 3 cut(s) 180, 758, 1204
MluCI AATT 7 cut(s) 382, 535, 877, 928, 1256, 1362, 1460
MlyI GAGTC 3 cut(s) 1000, 1376, 1399
MmeI TCCRAC 3 cut(s) 252, 473, 816
MnlI CCTC 9 cut(s) 140, 160, 388, 504, 712, 933, 1126, 1255, 1351
Mph1103I ATGCAT 1 cut(s) 1446
MroXI GAANNNNTTC 1 cut(s) 1472
MseI TTAA 3 cut(s) 92, 578, 1152
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 859
MspR9I CCNGG 2 cut(s) 156, 1322
MunI CAATTG 1 cut(s) 535
Mva1269I GAATGC 1 cut(s) 1444
MvaI CCWGG 2 cut(s) 156, 1322
MwoI GCNNNNNNNGC 5 cut(s) 423, 1040, 1082, 1303, 1441
NdeII GATC 6 cut(s) 16, 591, 711, 862, 1298, 1396
NlaIII CATG 8 cut(s) 55, 149, 184, 265, 412, 555, 808, 1121
NlaIV GGNNCC 2 cut(s) 688, 1044
NmuCI GTSAC 2 cut(s) 992, 1495
NsiI ATGCAT 1 cut(s) 1446
NspV TTCGAA 1 cut(s) 1448
PagI TCATGA 2 cut(s) 180, 1117
PciSI GCTCTTC 1 cut(s) 1264
PctI GAATGC 1 cut(s) 1444
PdmI GAANNNNTTC 1 cut(s) 1472
PfeI GAWTC 1 cut(s) 1112
PflFI GACNNNGTC 1 cut(s) 328
PkrI GCNGC 2 cut(s) 796, 1078
Ple19I CGATCG 1 cut(s) 594
PleI GAGTC 3 cut(s) 999, 1375, 1398
PpsI GAGTC 3 cut(s) 999, 1375, 1398
Ppu21I YACGTR 1 cut(s) 1549
PpuMI RGGWCCY 1 cut(s) 1229
PsiI TTATAA 2 cut(s) 522, 605
Psp124BI GAGCTC 2 cut(s) 180, 758
Psp5II RGGWCCY 1 cut(s) 1229
Psp6I CCWGG 2 cut(s) 154, 1320
PspGI CCWGG 2 cut(s) 154, 1320
PspN4I GGNNCC 2 cut(s) 688, 1044
PspPI GGNCC 1 cut(s) 1229
PspPPI RGGWCCY 1 cut(s) 1229
PstNI CAGNNNCTG 1 cut(s) 1103
PsyI GACNNNGTC 1 cut(s) 328
PvuI CGATCG 1 cut(s) 594
PvuII CAGCTG 1 cut(s) 859
RsaI GTAC 3 cut(s) 121, 707, 1317
RsaNI GTAC 3 cut(s) 120, 706, 1316
RseI CAYNNNNRTG 1 cut(s) 266
SacI GAGCTC 2 cut(s) 180, 758
SapI GCTCTTC 1 cut(s) 1264
SaqAI TTAA 3 cut(s) 92, 578, 1152
SatI GCNGC 2 cut(s) 795, 1077
Sau3AI GATC 6 cut(s) 16, 591, 711, 862, 1298, 1396
Sau96I GGNCC 1 cut(s) 1229
SchI GAGTC 3 cut(s) 1000, 1376, 1399
ScrFI CCNGG 2 cut(s) 156, 1322
SduI GDGCHC 3 cut(s) 180, 758, 1204
SfaNI GCATC 4 cut(s) 83, 258, 426, 1431
SfcI CTRYAG 1 cut(s) 639
SfuI TTCGAA 1 cut(s) 1448
SinI GGWCC 1 cut(s) 1229
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 7 cut(s) 382, 535, 877, 928, 1256, 1362, 1460
SsiI CCGC 1 cut(s) 794
SspMI CTAG 2 cut(s) 341, 501
SstI GAGCTC 2 cut(s) 180, 758
StyD4I CCNGG 2 cut(s) 154, 1320
StyI CCWWGG 2 cut(s) 621, 1344
TaaI ACNGT 2 cut(s) 636, 643
TaiI ACGT 3 cut(s) 329, 483, 1551
TaqI TCGA 5 cut(s) 88, 509, 590, 594, 1448
TaqII GACCGA 1 cut(s) 719
TasI AATT 7 cut(s) 382, 535, 877, 928, 1256, 1362, 1460
TatI WGTACW 1 cut(s) 705
TauI GCSGC 1 cut(s) 797
TfiI GAWTC 1 cut(s) 1112
Tru1I TTAA 3 cut(s) 92, 578, 1152
Tru9I TTAA 3 cut(s) 92, 578, 1152
TscAI CASTG 3 cut(s) 362, 1168, 1209
TseFI GTSAC 2 cut(s) 992, 1495
TseI GCWGC 1 cut(s) 1076
Tsp45I GTSAC 2 cut(s) 992, 1495
TspDTI ATGAA 7 cut(s) 40, 250, 378, 459, 560, 821, 1043
TspRI CASTG 3 cut(s) 362, 1168, 1209
Tth111I GACNNNGTC 1 cut(s) 328
VneI GTGCAC 1 cut(s) 1200
VpaK11BI GGWCC 1 cut(s) 1229
XapI RAATTY 3 cut(s) 382, 928, 1256
XcmI CCANNNNNNNNNTGG 2 cut(s) 152, 500
XmiI GTMKAC 4 cut(s) 243, 638, 733, 1329
XmnI GAANNNNTTC 1 cut(s) 1472
XspI CTAG 2 cut(s) 341, 501
Zsp2I ATGCAT 1 cut(s) 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.