Rorug04G0413300

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015568
Physical Location & Seq
Reverse (-)
57496986 .. 57498260
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0413300.1

Sequence Viewer

Length: 1071 bp
ATGGAGGTGGTGACGCGACTTACGCAAGTACTTCACATGAACAAAGGCGATGGTGAGACTAGTTATGCCAGAAACTCCACTGTTCAGAGAAAAACAATATCTATCGCCAAGCCAATCATTGAGGAAGCAGTACTAGAAATCTTGGGCTCAAATGTCATGGGAATAGAAAGCATGGGAATAGCAGATTTGGGTTGCTCATCAGGACCCAACACCTTCCTAGTCATCTCGCAGATCATCAATGTCATACATACTAGATGCAGCACCGGTCTTGATCATCCGTCGCCAGTAACAGAGCTTAGAGTATATCTAAATGACCTCTTTAGCAACGACTTCAACTCCGTCTTTATGTCACTCCCATCATTCTACAACAAACTGAGACAAGACTATGATAATGGTCGTGATCCGGATCAGCATGTCATACATCCCTTGATTTCTGCTGTGCCCGGTTCGTTTTATGGTCCACTATTTCCTAAAAAGAGTTTGCACTTTGTTCACTCTTCTTTTAGTCTTCACTGGCTCTCTCAGATCCCCGATGGCCTAAACAACAAAGGCAAGATTTACATTTCTAAGAGCAGCCCACAGTGTGTGTTGGATGCATACTCCATGCAGTTTCAGAAAGACTTTTCGGTTTTTCTGAGGTCTAGGGCACAAGAAATAGTTAACGGAGGTCGAATGGTGTTGTCCTTCTTGGGCAGGCCATCCTCTGACCCCACAGCTGATCAGGGCACTTTCTACCAGTTCGAGCTCTTAGCCCACGCATTAATGGACATGGTGTCCAAGGGCCTTATAGAAGAGGAAATGGTTGATTCCTTCGACATGCCCTACTATACTTCATGTGCAGAGGAATTGAGTTTGGTGTTACAAGAAGAAGGGTCTTTCATCAAGGACCGCCTTGAAGCATTCGAAGTGAATTGGGATACTGGTGCTGCTGATCTCGATCATAACATGCCTAACGTTAGGCCTAACGGTGCATTTGACGATGATCATACAATAACAAGCAGCGGGCAGCGAGTGGCCAATAGCATAAGAGTCGTGGCTGAGTCAATGCTTAAATCTCGAGATAATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

356

Amino Acids

39.65

Weight (kDa)

5.12

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 55 - 352 4.9e-103 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015568)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03040
malus_domestica MD05G1335300.v1.1
prunus_persica Prupe.4G030100_v2.0.a1 Prupe.4G030100_v2.0.a1 Prupe.4G030100_v2.0.a1 Prupe.4G030100_v2.0.a1
pyrus_communis pycom05g30670
rosa_chinensis RchiOBHm_Chr5g0004691
rosa_laevigata RLG00000031252
rosa_roxburghii Rroxscaffold_1G00070870
rosa_rugosa Rorug04G0413300
rosa_samantha Rh5AG040900 Rh5BG040600 Rh5CG044400 Rh5DG040600
rosa_wichuraiana Rw5G003940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 16
AccIII TCCGGA 1 cut(s) 403
AciI CCGC 2 cut(s) 889, 1002
AclI AACGTT 1 cut(s) 954
AclWI GGATC 3 cut(s) 395, 414, 520
AcoI YGGCCR 1 cut(s) 1014
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 2 cut(s) 30, 132
AgeI ACCGGT 1 cut(s) 263
AgsI TTSAA 2 cut(s) 334, 896
AhdI GACNNNNNGTC 1 cut(s) 772
AhlI ACTAGT 1 cut(s) 59
AluBI AGCT 3 cut(s) 295, 716, 745
AluI AGCT 3 cut(s) 295, 716, 745
Alw21I GWGCWC 1 cut(s) 747
Alw26I GTCTC 2 cut(s) 50, 370
AlwI GGATC 3 cut(s) 395, 414, 520
Ama87I CYCGRG 1 cut(s) 1056
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 5 cut(s) 535, 695, 781, 959, 1014
ApeKI GCWGC 5 cut(s) 258, 573, 926, 999, 1006
AseI ATTAAT 1 cut(s) 761
AsiGI ACCGGT 1 cut(s) 263
AspS9I GGNCC 4 cut(s) 203, 458, 781, 886
AsuC2I CCSGG 1 cut(s) 444
AsuHPI GGTGA 2 cut(s) 22, 65
AsuII TTCGAA 1 cut(s) 903
AvaI CYCGRG 1 cut(s) 1056
AvaII GGWCC 3 cut(s) 203, 458, 886
BaeGI GKGCMC 3 cut(s) 444, 649, 728
BalI TGGCCA 1 cut(s) 1016
BanII GRGCYC 2 cut(s) 149, 747
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 747
BbvI GCAGC 5 cut(s) 270, 585, 913, 1011, 1018
BccI CCATC 4 cut(s) 44, 364, 527, 706
BcgI CGANNNNNNTGC 2 cut(s) 1012, 1046
BciVI GTATCC 1 cut(s) 910
BclI TGATCA 3 cut(s) 271, 718, 982
BcnI CCSGG 1 cut(s) 444
BcoDI GTCTC 2 cut(s) 50, 370
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 5 cut(s) 60, 134, 218, 252, 642
BfuI GTATCC 1 cut(s) 910
BisI GCNGC 5 cut(s) 259, 574, 927, 1000, 1007
BlsI GCNGC 5 cut(s) 260, 575, 928, 1001, 1008
BmcAI AGTACT 2 cut(s) 30, 132
Bme1390I CCNGG 1 cut(s) 444
Bme18I GGWCC 3 cut(s) 203, 458, 886
BmeRI GACNNNNNGTC 1 cut(s) 772
BmeT110I CYCGRG 1 cut(s) 1056
BmgT120I GGNCC 4 cut(s) 203, 458, 781, 886
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 444
BmsI GCATC 2 cut(s) 245, 583
BpiI GAAGAC 1 cut(s) 500
Bpu14I TTCGAA 1 cut(s) 903
BpuMI CCSGG 1 cut(s) 444
BsaBI GATNNNNATC 2 cut(s) 405, 936
BsaJI CCNNGG 1 cut(s) 777
BsaWI WCCGGW 2 cut(s) 263, 403
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 4 cut(s) 284, 518, 736, 925
Bse8I GATNNNNATC 2 cut(s) 405, 936
BseAI TCCGGA 1 cut(s) 403
BseDI CCNNGG 1 cut(s) 777
BseGI GGATG 4 cut(s) 274, 421, 598, 698
BseJI GATNNNNATC 2 cut(s) 405, 936
BseMII CTCAG 4 cut(s) 365, 536, 626, 1029
BseNI ACTGG 4 cut(s) 284, 518, 736, 925
BseSI GKGCMC 3 cut(s) 444, 649, 728
BseXI GCAGC 5 cut(s) 270, 585, 913, 1011, 1018
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 5 cut(s) 537, 697, 783, 961, 1016
BshTI ACCGGT 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 747
BsiHKCI CYCGRG 1 cut(s) 1056
BsiSI CCGG 3 cut(s) 264, 404, 444
BsmAI GTCTC 2 cut(s) 50, 370
BsmI GAATGC 1 cut(s) 899
BsnI GGCC 5 cut(s) 537, 697, 783, 961, 1016
BsoBI CYCGRG 1 cut(s) 1056
Bsp119I TTCGAA 1 cut(s) 903
Bsp1286I GDGCHC 5 cut(s) 149, 444, 649, 728, 747
Bsp13I TCCGGA 1 cut(s) 403
Bsp143I GATC 9 cut(s) 231, 271, 400, 406, 525, 718, 931, 937, 982
BspACI CCGC 2 cut(s) 889, 1002
BspANI GGCC 5 cut(s) 537, 697, 783, 961, 1016
BspCNI CTCAG 4 cut(s) 366, 535, 627, 1030
BspEI TCCGGA 1 cut(s) 403
BspFNI CGCG 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 205
BspPI GGATC 3 cut(s) 395, 414, 520
BspT104I TTCGAA 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 4 cut(s) 284, 518, 736, 925
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 1 cut(s) 777
BssMI GATC 9 cut(s) 231, 271, 400, 406, 525, 718, 931, 937, 982
BssT1I CCWWGG 1 cut(s) 777
Bst4CI ACNGT 3 cut(s) 82, 582, 968
Bst6I CTCTTC 2 cut(s) 502, 786
BstBI TTCGAA 1 cut(s) 903
BstC8I GCNNGC 2 cut(s) 695, 1004
BstDEI CTNAG 7 cut(s) 296, 374, 522, 567, 635, 748, 1038
BstF5I GGATG 4 cut(s) 274, 421, 598, 698
BstFNI CGCG 1 cut(s) 16
BstKTI GATC 9 cut(s) 234, 274, 403, 409, 528, 721, 934, 940, 985
BstMAI GTCTC 2 cut(s) 50, 370
BstMBI GATC 9 cut(s) 231, 271, 400, 406, 525, 718, 931, 937, 982
BstMWI GCNNNNNNNGC 1 cut(s) 22
BstNSI RCATGY 3 cut(s) 416, 820, 949
BstSCI CCNGG 1 cut(s) 442
BstSLI GKGCMC 3 cut(s) 444, 649, 728
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 5 cut(s) 270, 585, 913, 1011, 1018
BstV2I GAAGAC 1 cut(s) 500
BstX2I RGATCY 1 cut(s) 525
BstYI RGATCY 1 cut(s) 525
BsuI GTATCC 1 cut(s) 910
BsuRI GGCC 5 cut(s) 537, 697, 783, 961, 1016
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 4 cut(s) 274, 421, 598, 698
BtsIMutI CAGTG 3 cut(s) 78, 511, 587
Cac8I GCNNGC 2 cut(s) 695, 1004
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 4 cut(s) 203, 458, 781, 886
CseI GACGC 1 cut(s) 22
Csp6I GTAC 2 cut(s) 29, 131
CspAI ACCGGT 1 cut(s) 263
CviAII CATG 9 cut(s) 37, 157, 172, 413, 604, 769, 817, 834, 946
CviQI GTAC 2 cut(s) 29, 131
DdeI CTNAG 7 cut(s) 296, 374, 522, 567, 635, 748, 1038
DpnI GATC 9 cut(s) 233, 273, 402, 408, 527, 720, 933, 939, 984
DpnII GATC 9 cut(s) 231, 271, 400, 406, 525, 718, 931, 937, 982
DraIII CACNNNGTG 1 cut(s) 584
DriI GACNNNNNGTC 1 cut(s) 772
EaeI YGGCCR 1 cut(s) 1014
Eam1104I CTCTTC 2 cut(s) 502, 786
Eam1105I GACNNNNNGTC 1 cut(s) 772
EarI CTCTTC 2 cut(s) 502, 786
Ecl136II GAGCTC 1 cut(s) 745
Eco130I CCWWGG 1 cut(s) 777
Eco147I AGGCCT 1 cut(s) 961
Eco24I GRGCYC 2 cut(s) 149, 747
Eco47I GGWCC 3 cut(s) 203, 458, 886
Eco53kI GAGCTC 1 cut(s) 745
Eco88I CYCGRG 1 cut(s) 1056
EcoICRI GAGCTC 1 cut(s) 745
EcoO109I RGGNCCY 2 cut(s) 203, 781
EcoT14I CCWWGG 1 cut(s) 777
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 2 cut(s) 149, 747
ErhI CCWWGG 1 cut(s) 777
FaeI CATG 9 cut(s) 40, 160, 175, 416, 607, 772, 820, 837, 949
FatI CATG 9 cut(s) 36, 156, 171, 412, 603, 768, 816, 833, 945
FauI CCCGC 1 cut(s) 995
FbaI TGATCA 3 cut(s) 271, 718, 982
Fnu4HI GCNGC 5 cut(s) 259, 574, 927, 1000, 1007
FokI GGATG 4 cut(s) 261, 408, 605, 685
FriOI GRGCYC 2 cut(s) 149, 747
Fsp4HI GCNGC 5 cut(s) 259, 574, 927, 1000, 1007
FspBI CTAG 5 cut(s) 60, 134, 218, 252, 642
GluI GCNGC 5 cut(s) 259, 574, 927, 1000, 1007
HaeIII GGCC 5 cut(s) 537, 697, 783, 961, 1016
HapII CCGG 3 cut(s) 264, 404, 444
HgaI GACGC 1 cut(s) 22
Hin1II CATG 9 cut(s) 40, 160, 175, 416, 607, 772, 820, 837, 949
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HinfI GANTC 3 cut(s) 806, 1029, 1040
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 3 cut(s) 264, 404, 444
HphI GGTGA 2 cut(s) 22, 65
Hpy166II GTNNAC 3 cut(s) 461, 493, 661
Hpy188I TCNGA 5 cut(s) 87, 525, 615, 636, 706
Hpy188III TCNNGA 7 cut(s) 201, 269, 398, 404, 935, 1056, 1058
Hpy8I GTNNAC 3 cut(s) 461, 493, 661
Hpy99I CGWCG 1 cut(s) 283
HpyAV CCTTC 4 cut(s) 223, 694, 820, 863
HpyCH4III ACNGT 3 cut(s) 82, 582, 968
HpyCH4IV ACGT 1 cut(s) 954
HpyCH4V TGCA 6 cut(s) 258, 484, 596, 607, 839, 971
HpyF10VI GCNNNNNNNGC 1 cut(s) 22
HpyF3I CTNAG 7 cut(s) 296, 374, 522, 567, 635, 748, 1038
HpySE526I ACGT 1 cut(s) 954
Hsp92II CATG 9 cut(s) 40, 160, 175, 416, 607, 772, 820, 837, 949
Kpn2I TCCGGA 1 cut(s) 403
Ksp22I TGATCA 3 cut(s) 271, 718, 982
KspAI GTTAAC 1 cut(s) 661
Kzo9I GATC 9 cut(s) 231, 271, 400, 406, 525, 718, 931, 937, 982
Lsp1109I GCAGC 5 cut(s) 270, 585, 913, 1011, 1018
LweI GCATC 2 cut(s) 245, 583
MaeI CTAG 5 cut(s) 60, 134, 218, 252, 642
MaeII ACGT 1 cut(s) 954
MaeIII GTNAC 4 cut(s) 10, 286, 348, 858
MalI GATC 9 cut(s) 233, 273, 402, 408, 527, 720, 933, 939, 984
MboI GATC 9 cut(s) 231, 271, 400, 406, 525, 718, 931, 937, 982
MboII GAAGA 4 cut(s) 489, 500, 803, 878
MflI RGATCY 1 cut(s) 525
MhlI GDGCHC 5 cut(s) 149, 444, 649, 728, 747
MlsI TGGCCA 1 cut(s) 1016
MluCI AATT 2 cut(s) 845, 910
MluNI TGGCCA 1 cut(s) 1016
MlyI GAGTC 2 cut(s) 1038, 1049
MmeI TCCRAC 1 cut(s) 570
MnlI CCTC 7 cut(s) 115, 326, 630, 659, 712, 787, 835
Mox20I TGGCCA 1 cut(s) 1016
Mph1103I ATGCAT 1 cut(s) 598
MroI TCCGGA 1 cut(s) 403
MscI TGGCCA 1 cut(s) 1016
MseI TTAA 3 cut(s) 660, 761, 1050
Msp20I TGGCCA 1 cut(s) 1016
MspA1I CMGCKG 2 cut(s) 716, 1002
MspI CCGG 3 cut(s) 264, 404, 444
MspR9I CCNGG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 899
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 1 cut(s) 22
NciI CCSGG 1 cut(s) 444
NdeII GATC 9 cut(s) 231, 271, 400, 406, 525, 718, 931, 937, 982
NlaIII CATG 9 cut(s) 40, 160, 175, 416, 607, 772, 820, 837, 949
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 10, 348
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 3 cut(s) 416, 820, 949
NspV TTCGAA 1 cut(s) 903
PaeR7I CTCGAG 1 cut(s) 1056
PceI AGGCCT 1 cut(s) 961
PctI GAATGC 1 cut(s) 899
PfeI GAWTC 1 cut(s) 806
PinAI ACCGGT 1 cut(s) 263
PkrI GCNGC 5 cut(s) 260, 575, 928, 1001, 1008
PleI GAGTC 2 cut(s) 1037, 1048
PpsI GAGTC 2 cut(s) 1037, 1048
PpuMI RGGWCCY 1 cut(s) 203
PshBI ATTAAT 1 cut(s) 761
Psp124BI GAGCTC 1 cut(s) 747
Psp1406I AACGTT 1 cut(s) 954
Psp5II RGGWCCY 1 cut(s) 203
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 4 cut(s) 203, 458, 781, 886
PspPPI RGGWCCY 1 cut(s) 203
PsuI RGATCY 1 cut(s) 525
PvuII CAGCTG 1 cut(s) 716
RsaI GTAC 2 cut(s) 30, 132
RsaNI GTAC 2 cut(s) 29, 131
SacI GAGCTC 1 cut(s) 747
SaqAI TTAA 3 cut(s) 660, 761, 1050
SatI GCNGC 5 cut(s) 259, 574, 927, 1000, 1007
Sau3AI GATC 9 cut(s) 231, 271, 400, 406, 525, 718, 931, 937, 982
Sau96I GGNCC 4 cut(s) 203, 458, 781, 886
ScaI AGTACT 2 cut(s) 30, 132
SchI GAGTC 2 cut(s) 1038, 1049
ScrFI CCNGG 1 cut(s) 444
SduI GDGCHC 5 cut(s) 149, 444, 649, 728, 747
SetI ASST 9 cut(s) 9, 215, 297, 318, 641, 670, 718, 747, 957
SfaNI GCATC 2 cut(s) 245, 583
Sfr274I CTCGAG 1 cut(s) 1056
SfuI TTCGAA 1 cut(s) 903
SinI GGWCC 3 cut(s) 203, 458, 886
SlaI CTCGAG 1 cut(s) 1056
SmlI CTYRAG 1 cut(s) 1056
SmoI CTYRAG 1 cut(s) 1056
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 2 cut(s) 845, 910
SseBI AGGCCT 1 cut(s) 961
SsiI CCGC 2 cut(s) 889, 1002
SspMI CTAG 5 cut(s) 60, 134, 218, 252, 642
SstI GAGCTC 1 cut(s) 747
StuI AGGCCT 1 cut(s) 961
StyD4I CCNGG 1 cut(s) 442
StyI CCWWGG 1 cut(s) 777
TaaI ACNGT 3 cut(s) 82, 582, 968
TaiI ACGT 1 cut(s) 957
TaqI TCGA 6 cut(s) 670, 741, 813, 903, 936, 1057
TasI AATT 2 cut(s) 845, 910
TatI WGTACW 2 cut(s) 28, 130
TfiI GAWTC 1 cut(s) 806
Tru1I TTAA 3 cut(s) 660, 761, 1050
Tru9I TTAA 3 cut(s) 660, 761, 1050
TscAI CASTG 3 cut(s) 85, 518, 587
TseFI GTSAC 2 cut(s) 10, 348
TseI GCWGC 5 cut(s) 258, 573, 926, 999, 1006
Tsp45I GTSAC 2 cut(s) 10, 348
TspDTI ATGAA 3 cut(s) 53, 822, 868
TspGWI ACGGA 3 cut(s) 267, 328, 678
TspRI CASTG 3 cut(s) 85, 518, 587
VpaK11BI GGWCC 3 cut(s) 203, 458, 886
VspI ATTAAT 1 cut(s) 761
XceI RCATGY 3 cut(s) 416, 820, 949
XhoI CTCGAG 1 cut(s) 1056
XspI CTAG 5 cut(s) 60, 134, 218, 252, 642
ZrmI AGTACT 2 cut(s) 30, 132
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.