Belongs to the glycosyl hydrolase 17 family
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g25740 |
| malus_domestica | MD06G1006900.v1.1 |
| prunus_persica | Prupe.5G008200_v2.0.a1 |
| pyrus_communis | pycom06g00600 |
| rosa_chinensis | RchiOBHm_Chr7g0216091 |
| rosa_laevigata | RLG00000002595 |
| rosa_multiflora | Rmu_sc0009767.1_g000003 Rmu_sc0024130.1_g000001 |
| rosa_roxburghii | Rroxscaffold_3G00243660 |
| rosa_rugosa | Rorug07G0158800 |
| rosa_samantha | Rh7AG295800 Rh7CG316200 Rh7DG298700 |
| rosa_wichuraiana | Rw7G025160 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 891 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 224 |
| Acc36I | ACCTGC | 1 cut(s) | 114 |
| AccB1I | GGYRCC | 1 cut(s) | 602 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1400 |
| AccII | CGCG | 2 cut(s) | 59, 1007 |
| AciI | CCGC | 5 cut(s) | 59, 278, 593, 771, 864 |
| AclWI | GGATC | 2 cut(s) | 389, 1385 |
| AcoI | YGGCCR | 3 cut(s) | 3, 504, 833 |
| AcsI | RAATTY | 2 cut(s) | 1018, 1076 |
| AcyI | GRCGYC | 1 cut(s) | 603 |
| AdeI | CACNNNGTG | 1 cut(s) | 448 |
| AfaI | GTAC | 3 cut(s) | 725, 731, 1055 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 58, 466, 613, 658, 805, 842, 1400 |
| AflIII | ACRYGT | 1 cut(s) | 726 |
| AgsI | TTSAA | 2 cut(s) | 1202, 1363 |
| AjiI | CACGTC | 1 cut(s) | 268 |
| AjnI | CCWGG | 3 cut(s) | 138, 192, 423 |
| AleI | CACNNNNGTG | 1 cut(s) | 266 |
| AluBI | AGCT | 9 cut(s) | 45, 128, 133, 374, 416, 581, 1071, 1205, 1288 |
| AluI | AGCT | 9 cut(s) | 45, 128, 133, 374, 416, 581, 1071, 1205, 1288 |
| Alw21I | GWGCWC | 1 cut(s) | 453 |
| Alw26I | GTCTC | 1 cut(s) | 253 |
| AlwI | GGATC | 2 cut(s) | 389, 1385 |
| Ama87I | CYCGRG | 1 cut(s) | 459 |
| AoxI | GGCC | 5 cut(s) | 3, 141, 195, 504, 833 |
| ApeKI | GCWGC | 6 cut(s) | 272, 275, 374, 581, 1071, 1083 |
| ApoI | RAATTY | 2 cut(s) | 1018, 1076 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 272, 304 |
| AspLEI | GCGC | 3 cut(s) | 474, 605, 1007 |
| AspS9I | GGNCC | 4 cut(s) | 406, 421, 801, 1151 |
| AsuC2I | CCSGG | 3 cut(s) | 644, 829, 853 |
| AsuHPI | GGTGA | 5 cut(s) | 105, 478, 857, 1136, 1277 |
| AsuII | TTCGAA | 1 cut(s) | 1046 |
| AvaI | CYCGRG | 1 cut(s) | 459 |
| AvaII | GGWCC | 4 cut(s) | 406, 421, 801, 1151 |
| BaeGI | GKGCMC | 2 cut(s) | 916, 1370 |
| BalI | TGGCCA | 2 cut(s) | 5, 835 |
| BanI | GGYRCC | 1 cut(s) | 602 |
| BauI | CACGAG | 1 cut(s) | 297 |
| Bbv12I | GWGCWC | 1 cut(s) | 453 |
| BbvI | GCAGC | 6 cut(s) | 259, 262, 361, 568, 1058, 1095 |
| BccI | CCATC | 5 cut(s) | 118, 335, 844, 1085, 1162 |
| BceAI | ACGGC | 2 cut(s) | 215, 491 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 254, 272, 288, 306 |
| BciT130I | CCWGG | 3 cut(s) | 140, 194, 425 |
| BciVI | GTATCC | 1 cut(s) | 1425 |
| BcnI | CCSGG | 3 cut(s) | 644, 829, 853 |
| BcoDI | GTCTC | 1 cut(s) | 253 |
| BfaI | CTAG | 3 cut(s) | 512, 683, 897 |
| BfmI | CTRYAG | 1 cut(s) | 342 |
| BfoI | RGCGCY | 1 cut(s) | 606 |
| BfuAI | ACCTGC | 1 cut(s) | 114 |
| BfuI | GTATCC | 1 cut(s) | 1425 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 590 |
| BglII | AGATCT | 1 cut(s) | 1251 |
| BisI | GCNGC | 8 cut(s) | 273, 276, 375, 582, 771, 864, 1072, 1084 |
| BlpI | GCTNAGC | 2 cut(s) | 129, 1173 |
| BlsI | GCNGC | 8 cut(s) | 274, 277, 376, 583, 772, 865, 1073, 1085 |
| Bme1390I | CCNGG | 6 cut(s) | 140, 194, 425, 644, 829, 853 |
| Bme18I | GGWCC | 4 cut(s) | 406, 421, 801, 1151 |
| BmeT110I | CYCGRG | 1 cut(s) | 459 |
| BmgBI | CACGTC | 1 cut(s) | 268 |
| BmgT120I | GGNCC | 4 cut(s) | 406, 421, 801, 1151 |
| BmiI | GGNNCC | 3 cut(s) | 408, 604, 1152 |
| BmrFI | CCNGG | 6 cut(s) | 140, 194, 425, 644, 829, 853 |
| BmrI | ACTGGG | 1 cut(s) | 910 |
| BmsI | GCATC | 8 cut(s) | 222, 524, 736, 748, 769, 1310, 1320, 1397 |
| BmuI | ACTGGG | 1 cut(s) | 910 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 112, 144 |
| BpmI | CTGGAG | 3 cut(s) | 122, 176, 488 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 129, 1173 |
| Bpu14I | TTCGAA | 1 cut(s) | 1046 |
| BpuMI | CCSGG | 3 cut(s) | 644, 829, 853 |
| BsaAI | YACGTR | 1 cut(s) | 729 |
| BsaHI | GRCGYC | 1 cut(s) | 603 |
| BsaJI | CCNNGG | 4 cut(s) | 460, 804, 840, 852 |
| BsaWI | WCCGGW | 2 cut(s) | 68, 798 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 58, 466, 613, 658, 805, 842, 1400 |
| Bse118I | RCCGGY | 1 cut(s) | 1410 |
| Bse1I | ACTGG | 4 cut(s) | 604, 916, 1352, 1375 |
| Bse3DI | GCAATG | 3 cut(s) | 336, 435, 677 |
| BseBI | CCWGG | 3 cut(s) | 140, 194, 425 |
| BseDI | CCNNGG | 4 cut(s) | 460, 804, 840, 852 |
| BseGI | GGATG | 4 cut(s) | 515, 533, 751, 836 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 58, 466, 613, 658, 805, 842, 1400 |
| BseMI | GCAATG | 3 cut(s) | 336, 435, 677 |
| BseMII | CTCAG | 3 cut(s) | 143, 764, 1295 |
| BseNI | ACTGG | 4 cut(s) | 604, 916, 1352, 1375 |
| BseRI | GAGGAG | 4 cut(s) | 14, 17, 125, 1122 |
| BseSI | GKGCMC | 2 cut(s) | 916, 1370 |
| BseXI | GCAGC | 6 cut(s) | 259, 262, 361, 568, 1058, 1095 |
| Bsh1236I | CGCG | 2 cut(s) | 59, 1007 |
| BshFI | GGCC | 5 cut(s) | 5, 143, 197, 506, 835 |
| BshNI | GGYRCC | 1 cut(s) | 602 |
| BsiHKAI | GWGCWC | 1 cut(s) | 453 |
| BsiHKCI | CYCGRG | 1 cut(s) | 459 |
| BsiSI | CCGG | 7 cut(s) | 69, 643, 799, 828, 852, 872, 1411 |
| BslFI | GGGAC | 4 cut(s) | 103, 659, 943, 1052 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 58, 466, 613, 658, 805, 842, 1400 |
| BsmAI | GTCTC | 1 cut(s) | 253 |
| BsmFI | GGGAC | 4 cut(s) | 103, 659, 943, 1052 |
| BsmI | GAATGC | 1 cut(s) | 1321 |
| BsnI | GGCC | 5 cut(s) | 5, 143, 197, 506, 835 |
| BsoBI | CYCGRG | 1 cut(s) | 459 |
| Bsp119I | TTCGAA | 1 cut(s) | 1046 |
| Bsp1286I | GDGCHC | 3 cut(s) | 453, 916, 1370 |
| Bsp143I | GATC | 5 cut(s) | 394, 477, 810, 1251, 1390 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 129, 1173 |
| BspACI | CCGC | 5 cut(s) | 59, 278, 593, 771, 864 |
| BspANI | GGCC | 5 cut(s) | 5, 143, 197, 506, 835 |
| BspCNI | CTCAG | 3 cut(s) | 142, 763, 1294 |
| BspFNI | CGCG | 2 cut(s) | 59, 1007 |
| BspLI | GGNNCC | 3 cut(s) | 408, 604, 1152 |
| BspMI | ACCTGC | 1 cut(s) | 114 |
| BspPI | GGATC | 2 cut(s) | 389, 1385 |
| BspT104I | TTCGAA | 1 cut(s) | 1046 |
| BspT107I | GGYRCC | 1 cut(s) | 602 |
| BsrDI | GCAATG | 3 cut(s) | 336, 435, 677 |
| BsrFI | RCCGGY | 1 cut(s) | 1410 |
| BsrI | ACTGG | 4 cut(s) | 604, 916, 1352, 1375 |
| BssAI | RCCGGY | 1 cut(s) | 1410 |
| BssECI | CCNNGG | 4 cut(s) | 460, 804, 840, 852 |
| BssMI | GATC | 5 cut(s) | 394, 477, 810, 1251, 1390 |
| BssNI | GRCGYC | 1 cut(s) | 603 |
| BssSI | CACGAG | 1 cut(s) | 297 |
| BssT1I | CCWWGG | 1 cut(s) | 840 |
| Bst2BI | CACGAG | 1 cut(s) | 297 |
| Bst2UI | CCWGG | 3 cut(s) | 140, 194, 425 |
| Bst4CI | ACNGT | 4 cut(s) | 704, 805, 1043, 1425 |
| BstACI | GRCGYC | 1 cut(s) | 603 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 439, 448, 1080, 1307 |
| BstBAI | YACGTR | 1 cut(s) | 729 |
| BstBI | TTCGAA | 1 cut(s) | 1046 |
| BstC8I | GCNNGC | 2 cut(s) | 444, 912 |
| BstDEI | CTNAG | 6 cut(s) | 31, 129, 750, 1173, 1281, 1375 |
| BstDSI | CCRYGG | 1 cut(s) | 804 |
| BstEII | GGTNACC | 1 cut(s) | 484 |
| BstF5I | GGATG | 4 cut(s) | 515, 533, 751, 836 |
| BstFNI | CGCG | 2 cut(s) | 59, 1007 |
| BstH2I | RGCGCY | 1 cut(s) | 606 |
| BstHHI | GCGC | 3 cut(s) | 474, 605, 1007 |
| BstKTI | GATC | 5 cut(s) | 397, 480, 813, 1254, 1393 |
| BstMAI | GTCTC | 1 cut(s) | 253 |
| BstMBI | GATC | 5 cut(s) | 394, 477, 810, 1251, 1390 |
| BstNI | CCWGG | 3 cut(s) | 140, 194, 425 |
| BstNSI | RCATGY | 1 cut(s) | 742 |
| BstPI | GGTNACC | 1 cut(s) | 484 |
| BstSCI | CCNGG | 6 cut(s) | 138, 192, 423, 642, 827, 851 |
| BstSFI | CTRYAG | 1 cut(s) | 342 |
| BstSLI | GKGCMC | 2 cut(s) | 916, 1370 |
| BstUI | CGCG | 2 cut(s) | 59, 1007 |
| BstV1I | GCAGC | 6 cut(s) | 259, 262, 361, 568, 1058, 1095 |
| BstX2I | RGATCY | 2 cut(s) | 394, 1251 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 418, 742, 1161 |
| BstYI | RGATCY | 2 cut(s) | 394, 1251 |
| BsuI | GTATCC | 1 cut(s) | 1425 |
| BsuRI | GGCC | 5 cut(s) | 5, 143, 197, 506, 835 |
| BtgI | CCRYGG | 1 cut(s) | 804 |
| BtrI | CACGTC | 1 cut(s) | 268 |
| BtsCI | GGATG | 4 cut(s) | 515, 533, 751, 836 |
| BtsI | GCAGTG | 1 cut(s) | 453 |
| BtsIMutI | CAGTG | 4 cut(s) | 453, 597, 1368, 1393 |
| BveI | ACCTGC | 1 cut(s) | 114 |
| Cac8I | GCNNGC | 2 cut(s) | 444, 912 |
| CfoI | GCGC | 3 cut(s) | 474, 605, 1007 |
| Cfr10I | RCCGGY | 1 cut(s) | 1410 |
| Cfr13I | GGNCC | 4 cut(s) | 406, 421, 801, 1151 |
| CpoI | CGGWCCG | 1 cut(s) | 801 |
| Csp6I | GTAC | 3 cut(s) | 724, 730, 1054 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 538, 573 |
| CspI | CGGWCCG | 1 cut(s) | 801 |
| CviAII | CATG | 9 cut(s) | 245, 502, 658, 739, 814, 937, 1181, 1298, 1334 |
| CviQI | GTAC | 3 cut(s) | 724, 730, 1054 |
| DdeI | CTNAG | 6 cut(s) | 31, 129, 750, 1173, 1281, 1375 |
| DinI | GGCGCC | 1 cut(s) | 604 |
| DpnI | GATC | 5 cut(s) | 396, 479, 812, 1253, 1392 |
| DpnII | GATC | 5 cut(s) | 394, 477, 810, 1251, 1390 |
| DraIII | CACNNNGTG | 1 cut(s) | 448 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 224 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 224 |
| EaeI | YGGCCR | 3 cut(s) | 3, 504, 833 |
| EciI | GGCGGA | 1 cut(s) | 608 |
| Eco130I | CCWWGG | 1 cut(s) | 840 |
| Eco147I | AGGCCT | 1 cut(s) | 197 |
| Eco47I | GGWCC | 4 cut(s) | 406, 421, 801, 1151 |
| Eco88I | CYCGRG | 1 cut(s) | 459 |
| Eco91I | GGTNACC | 1 cut(s) | 484 |
| EcoO65I | GGTNACC | 1 cut(s) | 484 |
| EcoRII | CCWGG | 3 cut(s) | 138, 192, 423 |
| EcoT14I | CCWWGG | 1 cut(s) | 840 |
| EcoT22I | ATGCAT | 2 cut(s) | 1303, 1335 |
| EgeI | GGCGCC | 1 cut(s) | 604 |
| EheI | GGCGCC | 1 cut(s) | 604 |
| ErhI | CCWWGG | 1 cut(s) | 840 |
| FaeI | CATG | 9 cut(s) | 248, 505, 661, 742, 817, 940, 1184, 1301, 1337 |
| FaqI | GGGAC | 4 cut(s) | 103, 659, 943, 1052 |
| FatI | CATG | 9 cut(s) | 244, 501, 657, 738, 813, 936, 1180, 1297, 1333 |
| Fnu4HI | GCNGC | 8 cut(s) | 273, 276, 375, 582, 771, 864, 1072, 1084 |
| FokI | GGATG | 4 cut(s) | 502, 520, 758, 823 |
| Fsp4HI | GCNGC | 8 cut(s) | 273, 276, 375, 582, 771, 864, 1072, 1084 |
| FspBI | CTAG | 3 cut(s) | 512, 683, 897 |
| GlaI | GCGC | 3 cut(s) | 473, 604, 1006 |
| GluI | GCNGC | 8 cut(s) | 273, 276, 375, 582, 771, 864, 1072, 1084 |
| GsuI | CTGGAG | 3 cut(s) | 122, 176, 488 |
| HaeII | RGCGCY | 1 cut(s) | 606 |
| HaeIII | GGCC | 5 cut(s) | 5, 143, 197, 506, 835 |
| HapII | CCGG | 7 cut(s) | 69, 643, 799, 828, 852, 872, 1411 |
| HhaI | GCGC | 3 cut(s) | 474, 605, 1007 |
| Hin1I | GRCGYC | 1 cut(s) | 603 |
| Hin1II | CATG | 9 cut(s) | 248, 505, 661, 742, 817, 940, 1184, 1301, 1337 |
| Hin6I | GCGC | 3 cut(s) | 472, 603, 1005 |
| HinP1I | GCGC | 3 cut(s) | 472, 603, 1005 |
| HindIII | AAGCTT | 1 cut(s) | 1286 |
| HinfI | GANTC | 2 cut(s) | 91, 824 |
| HpaII | CCGG | 7 cut(s) | 69, 643, 799, 828, 852, 872, 1411 |
| HphI | GGTGA | 5 cut(s) | 105, 478, 857, 1136, 1277 |
| Hpy166II | GTNNAC | 4 cut(s) | 406, 726, 732, 1054 |
| Hpy188I | TCNGA | 6 cut(s) | 98, 753, 788, 823, 1284, 1329 |
| Hpy188III | TCNNGA | 2 cut(s) | 235, 1024 |
| Hpy8I | GTNNAC | 4 cut(s) | 406, 726, 732, 1054 |
| Hpy99I | CGWCG | 3 cut(s) | 230, 236, 269 |
| HpyAV | CCTTC | 7 cut(s) | 319, 540, 595, 988, 992, 1042, 1186 |
| HpyCH4III | ACNGT | 4 cut(s) | 704, 805, 1043, 1425 |
| HpyCH4IV | ACGT | 2 cut(s) | 267, 728 |
| HpyCH4V | TGCA | 8 cut(s) | 433, 442, 782, 1074, 1083, 1245, 1301, 1333 |
| HpyF3I | CTNAG | 6 cut(s) | 31, 129, 750, 1173, 1281, 1375 |
| HpySE526I | ACGT | 2 cut(s) | 267, 728 |
| Hsp92I | GRCGYC | 1 cut(s) | 603 |
| Hsp92II | CATG | 9 cut(s) | 248, 505, 661, 742, 817, 940, 1184, 1301, 1337 |
| HspAI | GCGC | 3 cut(s) | 472, 603, 1005 |
| KasI | GGCGCC | 1 cut(s) | 602 |
| Kzo9I | GATC | 5 cut(s) | 394, 477, 810, 1251, 1390 |
| LmnI | GCTCC | 2 cut(s) | 138, 469 |
| Lsp1109I | GCAGC | 6 cut(s) | 259, 262, 361, 568, 1058, 1095 |
| LweI | GCATC | 8 cut(s) | 222, 524, 736, 748, 769, 1310, 1320, 1397 |
| MaeI | CTAG | 3 cut(s) | 512, 683, 897 |
| MaeII | ACGT | 2 cut(s) | 267, 728 |
| MaeIII | GTNAC | 6 cut(s) | 386, 484, 710, 845, 1355, 1378 |
| MalI | GATC | 5 cut(s) | 396, 479, 812, 1253, 1392 |
| MboI | GATC | 5 cut(s) | 394, 477, 810, 1251, 1390 |
| MflI | RGATCY | 2 cut(s) | 394, 1251 |
| MhlI | GDGCHC | 3 cut(s) | 453, 916, 1370 |
| MlsI | TGGCCA | 2 cut(s) | 5, 835 |
| MluCI | AATT | 4 cut(s) | 36, 1018, 1076, 1351 |
| MluNI | TGGCCA | 2 cut(s) | 5, 835 |
| Mly113I | GGCGCC | 1 cut(s) | 603 |
| MlyI | GAGTC | 1 cut(s) | 85 |
| MmeI | TCCRAC | 4 cut(s) | 228, 276, 1191, 1418 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 835 |
| Mph1103I | ATGCAT | 2 cut(s) | 1303, 1335 |
| MscI | TGGCCA | 2 cut(s) | 5, 835 |
| MseI | TTAA | 3 cut(s) | 359, 981, 1032 |
| MslI | CAYNNNNRTG | 3 cut(s) | 266, 737, 1185 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 835 |
| MspA1I | CMGCKG | 1 cut(s) | 374 |
| MspI | CCGG | 7 cut(s) | 69, 643, 799, 828, 852, 872, 1411 |
| MspR9I | CCNGG | 6 cut(s) | 140, 194, 425, 644, 829, 853 |
| Mva1269I | GAATGC | 1 cut(s) | 1321 |
| MvaI | CCWGG | 3 cut(s) | 140, 194, 425 |
| MvnI | CGCG | 2 cut(s) | 59, 1007 |
| NarI | GGCGCC | 1 cut(s) | 603 |
| NciI | CCSGG | 3 cut(s) | 644, 829, 853 |
| NdeII | GATC | 5 cut(s) | 394, 477, 810, 1251, 1390 |
| NlaIII | CATG | 9 cut(s) | 248, 505, 661, 742, 817, 940, 1184, 1301, 1337 |
| NlaIV | GGNNCC | 3 cut(s) | 408, 604, 1152 |
| NmuCI | GTSAC | 5 cut(s) | 386, 484, 710, 845, 1355 |
| NsiI | ATGCAT | 2 cut(s) | 1303, 1335 |
| NspI | RCATGY | 1 cut(s) | 742 |
| NspV | TTCGAA | 1 cut(s) | 1046 |
| OliI | CACNNNNGTG | 1 cut(s) | 266 |
| PceI | AGGCCT | 1 cut(s) | 197 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 222, 231 |
| PctI | GAATGC | 1 cut(s) | 1321 |
| PfeI | GAWTC | 1 cut(s) | 824 |
| PflFI | GACNNNGTC | 2 cut(s) | 158, 659 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1400 |
| PkrI | GCNGC | 8 cut(s) | 274, 277, 376, 583, 772, 865, 1073, 1085 |
| PleI | GAGTC | 1 cut(s) | 85 |
| PluTI | GGCGCC | 1 cut(s) | 606 |
| PpsI | GAGTC | 1 cut(s) | 85 |
| Ppu21I | YACGTR | 1 cut(s) | 729 |
| PsiI | TTATAA | 1 cut(s) | 891 |
| Psp6I | CCWGG | 3 cut(s) | 138, 192, 423 |
| PspEI | GGTNACC | 1 cut(s) | 484 |
| PspGI | CCWGG | 3 cut(s) | 138, 192, 423 |
| PspN4I | GGNNCC | 3 cut(s) | 408, 604, 1152 |
| PspPI | GGNCC | 4 cut(s) | 406, 421, 801, 1151 |
| PsuI | RGATCY | 2 cut(s) | 394, 1251 |
| PsyI | GACNNNGTC | 2 cut(s) | 158, 659 |
| PvuII | CAGCTG | 1 cut(s) | 374 |
| RsaI | GTAC | 3 cut(s) | 725, 731, 1055 |
| RsaNI | GTAC | 3 cut(s) | 724, 730, 1054 |
| RseI | CAYNNNNRTG | 3 cut(s) | 266, 737, 1185 |
| Rsr2I | CGGWCCG | 1 cut(s) | 801 |
| RsrII | CGGWCCG | 1 cut(s) | 801 |
| SaqAI | TTAA | 3 cut(s) | 359, 981, 1032 |
| SatI | GCNGC | 8 cut(s) | 273, 276, 375, 582, 771, 864, 1072, 1084 |
| Sau3AI | GATC | 5 cut(s) | 394, 477, 810, 1251, 1390 |
| Sau96I | GGNCC | 4 cut(s) | 406, 421, 801, 1151 |
| SchI | GAGTC | 1 cut(s) | 85 |
| ScrFI | CCNGG | 6 cut(s) | 140, 194, 425, 644, 829, 853 |
| SduI | GDGCHC | 3 cut(s) | 453, 916, 1370 |
| SfaNI | GCATC | 8 cut(s) | 222, 524, 736, 748, 769, 1310, 1320, 1397 |
| SfcI | CTRYAG | 1 cut(s) | 342 |
| SfoI | GGCGCC | 1 cut(s) | 604 |
| SfuI | TTCGAA | 1 cut(s) | 1046 |
| SinI | GGWCC | 4 cut(s) | 406, 421, 801, 1151 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 266, 737, 1185 |
| Sse9I | AATT | 4 cut(s) | 36, 1018, 1076, 1351 |
| SseBI | AGGCCT | 1 cut(s) | 197 |
| SsiI | CCGC | 5 cut(s) | 59, 278, 593, 771, 864 |
| SspDI | GGCGCC | 1 cut(s) | 602 |
| SspMI | CTAG | 3 cut(s) | 512, 683, 897 |
| StuI | AGGCCT | 1 cut(s) | 197 |
| StyD4I | CCNGG | 6 cut(s) | 138, 192, 423, 642, 827, 851 |
| StyI | CCWWGG | 1 cut(s) | 840 |
| TaaI | ACNGT | 4 cut(s) | 704, 805, 1043, 1425 |
| TaiI | ACGT | 2 cut(s) | 270, 731 |
| TaqI | TCGA | 4 cut(s) | 216, 234, 766, 1046 |
| TasI | AATT | 4 cut(s) | 36, 1018, 1076, 1351 |
| TauI | GCSGC | 2 cut(s) | 773, 866 |
| TfiI | GAWTC | 1 cut(s) | 824 |
| Tru1I | TTAA | 3 cut(s) | 359, 981, 1032 |
| Tru9I | TTAA | 3 cut(s) | 359, 981, 1032 |
| TscAI | CASTG | 4 cut(s) | 453, 604, 1375, 1393 |
| TseFI | GTSAC | 5 cut(s) | 386, 484, 710, 845, 1355 |
| TseI | GCWGC | 6 cut(s) | 272, 275, 374, 581, 1071, 1083 |
| Tsp45I | GTSAC | 5 cut(s) | 386, 484, 710, 845, 1355 |
| TspDTI | ATGAA | 5 cut(s) | 301, 366, 522, 953, 1328 |
| TspGWI | ACGGA | 1 cut(s) | 1113 |
| TspRI | CASTG | 4 cut(s) | 453, 604, 1375, 1393 |
| Tth111I | GACNNNGTC | 2 cut(s) | 158, 659 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1400 |
| VpaK11BI | GGWCC | 4 cut(s) | 406, 421, 801, 1151 |
| XapI | RAATTY | 2 cut(s) | 1018, 1076 |
| XceI | RCATGY | 1 cut(s) | 742 |
| XspI | CTAG | 3 cut(s) | 512, 683, 897 |
| Zsp2I | ATGCAT | 2 cut(s) | 1303, 1335 |