Prupe.5G055800_v2.0.a1

Olee1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0021237
Physical Location & Seq
Forward (+)
6010031 .. 6010992
962 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G055800.1

Sequence Viewer

Length: 531 bp
ATGTCGACAAAGTCTAGCACTACTGTCCTTGCCTTTGCCTTTTGTTTCTTGTCCCTCCTCAGTTTCGCTTACTCCAACACCACCGACGACAAAATCTACCTCACCGGCCTGGTCTACTGTGACAACTGCCAATTGAAGTCTATGACCGAGATCAGTAAGATGATTCCAGGTGCAAGGGTGCGATTGGAGTGTAGGGAGGGGGAGAACATAAAATCCAGACGAGAGGGCGAAACCAACCCGCTTGGAATGTATGTGTTTGTGTTGGAAAAATCTAAGGAGCCTTTGGATGATTGTCAAGTGACAGTGCTACACAGCCCTGACCCTGAATGCAAGATTTCCCACGAGGTCGACCCTAACAACAAATCGAAAACTGCGCCGGTGGCCACTCGGAAGCTCAACCTTTTGGAAGGAGACAGTGTCGTTTTCATCGGACCAAGCCACCGCGTTTCCTACCCTCTTGGTTTGGTCGTTGAGAAAGCTCGTCCTGAGTGCGAGCAGTTCGACAAAGCACGCAAGCATTTTCAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

177

Amino Acids

19.77

Weight (kDa)

6.89

Isoelectric Point (pI)

37.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 5, 114, 348
AccII CGCG 1 cut(s) 444
AciI CCGC 2 cut(s) 239, 442
AcoI YGGCCR 1 cut(s) 381
AgsI TTSAA 1 cut(s) 136
AjnI CCWGG 2 cut(s) 108, 166
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 2 cut(s) 394, 479
AluI AGCT 2 cut(s) 394, 479
Alw26I GTCTC 1 cut(s) 405
AoxI GGCC 2 cut(s) 106, 381
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 1 cut(s) 431
AsuHPI GGTGA 1 cut(s) 94
AvaII GGWCC 1 cut(s) 431
BalI TGGCCA 1 cut(s) 383
BauI CACGAG 1 cut(s) 341
BciT130I CCWGG 2 cut(s) 110, 168
BcoDI GTCTC 1 cut(s) 405
BfaI CTAG 1 cut(s) 15
Bme1390I CCNGG 2 cut(s) 110, 168
Bme18I GGWCC 1 cut(s) 431
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 2 cut(s) 110, 168
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bse118I RCCGGY 2 cut(s) 104, 376
BseBI CCWGG 2 cut(s) 110, 168
BseGI GGATG 1 cut(s) 292
BseMII CTCAG 2 cut(s) 73, 477
BseRI GAGGAG 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 2 cut(s) 108, 383
BsiSI CCGG 2 cut(s) 105, 377
BslFI GGGAC 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 405
BsmFI GGGAC 1 cut(s) 37
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 2 cut(s) 108, 383
Bsp143I GATC 1 cut(s) 150
BspACI CCGC 2 cut(s) 239, 442
BspANI GGCC 2 cut(s) 108, 383
BspCNI CTCAG 2 cut(s) 72, 478
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 279
BsrFI RCCGGY 2 cut(s) 104, 376
BssAI RCCGGY 2 cut(s) 104, 376
BssMI GATC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 341
Bst2BI CACGAG 1 cut(s) 341
Bst2UI CCWGG 2 cut(s) 110, 168
Bst4CI ACNGT 4 cut(s) 25, 119, 304, 416
BstC8I GCNNGC 3 cut(s) 494, 511, 515
BstDEI CTNAG 3 cut(s) 59, 273, 486
BstF5I GGATG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 444
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 1 cut(s) 153
BstMAI GTCTC 1 cut(s) 405
BstMBI GATC 1 cut(s) 150
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 2 cut(s) 110, 168
BstSCI CCNGG 2 cut(s) 108, 166
BstUI CGCG 1 cut(s) 444
BsuRI GGCC 2 cut(s) 108, 383
BtsCI GGATG 1 cut(s) 292
BtsIMutI CAGTG 2 cut(s) 309, 421
Cac8I GCNNGC 3 cut(s) 494, 511, 515
CfoI GCGC 1 cut(s) 376
Cfr10I RCCGGY 2 cut(s) 104, 376
Cfr13I GGNCC 1 cut(s) 431
CviJI RGCY 7 cut(s) 108, 280, 315, 383, 394, 438, 479
CviKI_1 RGCY 7 cut(s) 108, 280, 315, 383, 394, 438, 479
DdeI CTNAG 3 cut(s) 59, 273, 486
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 108, 166
FaiI YATR 3 cut(s) 143, 209, 252
FaqI GGGAC 1 cut(s) 37
FauI CCCGC 1 cut(s) 246
FblI GTMKAC 3 cut(s) 5, 114, 348
FokI GGATG 1 cut(s) 299
FspBI CTAG 1 cut(s) 15
GlaI GCGC 1 cut(s) 375
HaeIII GGCC 2 cut(s) 108, 383
HapII CCGG 2 cut(s) 105, 377
HhaI GCGC 1 cut(s) 376
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HincII GTYRAC 2 cut(s) 6, 349
HindII GTYRAC 2 cut(s) 6, 349
HinfI GANTC 1 cut(s) 163
HpaII CCGG 2 cut(s) 105, 377
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 3 cut(s) 6, 115, 349
Hpy188I TCNGA 3 cut(s) 390, 431, 525
Hpy188III TCNNGA 2 cut(s) 216, 485
Hpy8I GTNNAC 3 cut(s) 6, 115, 349
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 401
HpyCH4III ACNGT 4 cut(s) 25, 119, 304, 416
HpyCH4V TGCA 2 cut(s) 173, 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpyF3I CTNAG 3 cut(s) 59, 273, 486
HspAI GCGC 1 cut(s) 374
Kzo9I GATC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 277
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 2 cut(s) 119, 298
MalI GATC 1 cut(s) 152
MboI GATC 1 cut(s) 150
MfeI CAATTG 1 cut(s) 131
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 1 cut(s) 131
MluNI TGGCCA 1 cut(s) 383
MmeI TCCRAC 2 cut(s) 99, 243
MnlI CCTC 7 cut(s) 65, 68, 110, 190, 217, 337, 465
Mox20I TGGCCA 1 cut(s) 383
MscI TGGCCA 1 cut(s) 383
Msp20I TGGCCA 1 cut(s) 383
MspI CCGG 2 cut(s) 105, 377
MspR9I CCNGG 2 cut(s) 110, 168
MunI CAATTG 1 cut(s) 131
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 2 cut(s) 110, 168
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 1 cut(s) 380
NdeII GATC 1 cut(s) 150
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 2 cut(s) 119, 298
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 1 cut(s) 163
PflFI GACNNNGTC 2 cut(s) 10, 416
Psp6I CCWGG 2 cut(s) 108, 166
PspGI CCWGG 2 cut(s) 108, 166
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 431
PsyI GACNNNGTC 2 cut(s) 10, 416
SalI GTCGAC 2 cut(s) 4, 347
Sau3AI GATC 1 cut(s) 150
Sau96I GGNCC 1 cut(s) 431
ScrFI CCNGG 2 cut(s) 110, 168
SetI ASST 6 cut(s) 102, 172, 348, 396, 402, 481
SgrAI CRCCGGYG 1 cut(s) 376
SinI GGWCC 1 cut(s) 431
Sse9I AATT 1 cut(s) 131
SsiI CCGC 2 cut(s) 239, 442
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 2 cut(s) 108, 166
TaaI ACNGT 4 cut(s) 25, 119, 304, 416
TaqI TCGA 4 cut(s) 5, 348, 365, 501
TaqII GACCGA 1 cut(s) 161
TasI AATT 1 cut(s) 131
TfiI GAWTC 1 cut(s) 163
TscAI CASTG 2 cut(s) 309, 421
TseFI GTSAC 2 cut(s) 119, 298
Tsp45I GTSAC 2 cut(s) 119, 298
TspDTI ATGAA 1 cut(s) 415
TspRI CASTG 2 cut(s) 309, 421
Tth111I GACNNNGTC 2 cut(s) 10, 416
VpaK11BI GGWCC 1 cut(s) 431
XmiI GTMKAC 3 cut(s) 5, 114, 348
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.