Prupe.5G129700_v2.0.a1

embryogenesis-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0006715
Physical Location & Seq
Forward (+)
12542375 .. 12546387
4013 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G129700.1

Sequence Viewer

Length: 1260 bp
ATGGCCAGAGCATCCCTGGGCTATTCTCCTCCACCTCTCTCCTACTTCCGCCACGTGTCTCACCTCCACAACTACACTCGCCTCGCCGCCACATCCGCATTCAACGTCAGCTCAGCCGCCGACCCGATGGCCCACCCGTCCCTCGAAACCCTCGGCGGCGCGCGCGACCTCTTCCTTCCCGCCCTCAGCTCACTGACCCGACCCTACAACCCGTTTCCCGTAATCGGGTCGAACCGCCACGTGGAGACCATATTCGCCTCGTTCTATAGGTCGACTCCCGACGTCAGGCTCCGCCGCGAGTGCCTCCGAACGACAGACGACGGCGCCGTTTCGCTCGATTGGGTCTCCAGTGACGACCGCCTCTTGCCTCCCGACTCCCCACTCCTCATTCTCCTGCCAGGTTTAACTGGAGGGAGTGATGACTCGTATGTGAGGCACATGTTGGTTCGAGCCAGAAGTAAAGGGTGGCGGGTTGTGGTGTTCAATAGCCGTGGTTGTGGTGGTAGCCCTGTAACAACTCCTCAGTTCTATTCGGCTTCATTTTTAGGAGACATGCGTGAAGTAGTGGCCCATGTTGGTAATCGGTATCCGAAAGCTAATCTGTATGCTGTTGGTTGGTCTCTTGGTGCTAACATTCTTGTTCGGTATTTGGGTCAGGAATCTGATGCATGTCGTCTTTCTGGTGCTGTGTCATTGTGTAATCCTTTCAATTTGGTCATTGCAGACGAGGACTTCCGTAAGGGCTTTAATATTGTTTATGACAAAGCTCTTGCAAGTGCTCTCTCCAAAATTTTCAAGAAGCATGCTCTACTCTTTGAAGACATGGGTGGTGATTTTAACATTCCATTGGCTGCCAATGCTAAGTCTGTCAGGGAGTTTGATGATGGACTAACTCGTGTTTCATTTGGTTTCAAGTCTGTAGATGACTACTACTCTAATTCCAGTAGTTCCAATTCCATAAAGGATGTCTGCATACCATTGCTTTGCATTCAGGCGGGAAATGATCCAATTGCCCCGAACAGGGGAATTCCTCGTGAAGATATCAAGGACAATCCAAACTGTATGTTGATAGTGACACCCAAAGGTGGCCATCTAGGGTGGGTTGCAGGTCCTGAAGCTCCATTCGGAGCTCCTTGGACTGATCCAGTTGTCATGGATTTCCTTCAGCATCTGGAGAGAGGAAAATCTGGAGCCCTTTCCTCTATTAACTCGGAAAGTGAGCAACGACGTTCGGAGGGTTTGCACCAATTGGAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

420

Amino Acids

45.78

Weight (kDa)

6.83

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 1097
AccB1I GGYRCC 1 cut(s) 323
AccI GTMKAC 1 cut(s) 272
AccII CGCG 4 cut(s) 161, 163, 165, 297
AclWI GGATC 2 cut(s) 998, 1136
AcoI YGGCCR 2 cut(s) 3, 1087
AcsI RAATTY 2 cut(s) 789, 1026
AcuI CTGAAG 2 cut(s) 1134, 1148
AcvI CACGTG 2 cut(s) 55, 241
AcyI GRCGYC 2 cut(s) 282, 324
AfiI CCNNNNNNNGG 8 cut(s) 224, 225, 241, 285, 1020, 1021, 1022, 1085
AflIII ACRYGT 2 cut(s) 54, 438
AgsI TTSAA 6 cut(s) 103, 484, 709, 796, 818, 913
AjnI CCWGG 2 cut(s) 15, 397
AluBI AGCT 6 cut(s) 111, 189, 596, 767, 1118, 1130
AluI AGCT 6 cut(s) 111, 189, 596, 767, 1118, 1130
Alw21I GWGCWC 2 cut(s) 781, 1132
Alw26I GTCTC 5 cut(s) 63, 239, 349, 543, 624
AlwI GGATC 2 cut(s) 998, 1136
AlwNI CAGNNNCTG 2 cut(s) 1112, 1171
AoxI GGCC 4 cut(s) 3, 129, 567, 1087
ApeKI GCWGC 1 cut(s) 851
ApoI RAATTY 2 cut(s) 789, 1026
AspLEI GCGC 4 cut(s) 161, 163, 165, 326
AspS9I GGNCC 3 cut(s) 130, 568, 1109
AsuHPI GGTGA 2 cut(s) 53, 842
AvaII GGWCC 1 cut(s) 1109
BalI TGGCCA 2 cut(s) 5, 1089
BanI GGYRCC 1 cut(s) 323
BanII GRGCYC 2 cut(s) 1132, 1195
BauI CACGAG 2 cut(s) 894, 1032
BbrPI CACGTG 2 cut(s) 55, 241
BbsI GAAGAC 1 cut(s) 825
Bbv12I GWGCWC 2 cut(s) 781, 1132
BbvCI CCTCAGC 1 cut(s) 185
BbvI GCAGC 1 cut(s) 838
BccI CCATC 3 cut(s) 121, 878, 1098
BceAI ACGGC 3 cut(s) 311, 337, 474
BciT130I CCWGG 2 cut(s) 17, 399
BciVI GTATCC 1 cut(s) 597
BcoDI GTCTC 5 cut(s) 63, 239, 349, 543, 624
BfaI CTAG 1 cut(s) 1094
BfmI CTRYAG 2 cut(s) 265, 918
BfoI RGCGCY 1 cut(s) 327
BfuAI ACCTGC 1 cut(s) 1097
BfuI GTATCC 1 cut(s) 597
BisI GCNGC 5 cut(s) 87, 117, 157, 295, 852
BlpI GCTNAGC 1 cut(s) 112
BlsI GCNGC 5 cut(s) 88, 118, 158, 296, 853
Bme1390I CCNGG 2 cut(s) 17, 399
Bme18I GGWCC 1 cut(s) 1109
BmgT120I GGNCC 3 cut(s) 130, 568, 1109
BmiI GGNNCC 3 cut(s) 290, 325, 1192
BmrFI CCNGG 2 cut(s) 17, 399
BmsI GCATC 3 cut(s) 20, 655, 1177
BpiI GAAGAC 1 cut(s) 825
BpmI CTGGAG 4 cut(s) 331, 429, 1193, 1209
Bpu10I CCTNAGC 1 cut(s) 185
Bpu1102I GCTNAGC 1 cut(s) 112
BsaAI YACGTR 2 cut(s) 55, 241
BsaHI GRCGYC 2 cut(s) 282, 324
BsaI GGTCTC 3 cut(s) 239, 349, 624
BsaJI CCNNGG 5 cut(s) 15, 16, 151, 490, 1133
Bsc4I CCNNNNNNNGG 8 cut(s) 224, 225, 241, 285, 1020, 1021, 1022, 1085
Bse1I ACTGG 4 cut(s) 348, 412, 942, 1145
Bse3DI GCAATG 2 cut(s) 717, 977
BseBI CCWGG 2 cut(s) 17, 399
BseDI CCNNGG 5 cut(s) 15, 16, 151, 490, 1133
BseGI GGATG 3 cut(s) 11, 92, 970
BseLI CCNNNNNNNGG 8 cut(s) 224, 225, 241, 285, 1020, 1021, 1022, 1085
BseMI GCAATG 2 cut(s) 717, 977
BseMII CTCAG 3 cut(s) 126, 199, 536
BseNI ACTGG 4 cut(s) 348, 412, 942, 1145
BsePI GCGCGC 2 cut(s) 159, 161
BseRI GAGGAG 3 cut(s) 18, 374, 510
BseXI GCAGC 1 cut(s) 838
Bsh1236I CGCG 4 cut(s) 161, 163, 165, 297
Bsh1285I CGRYCG 1 cut(s) 358
BshFI GGCC 4 cut(s) 5, 131, 569, 1089
BshNI GGYRCC 1 cut(s) 323
BsiEI CGRYCG 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 781, 1132
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 8 cut(s) 224, 225, 241, 285, 1020, 1021, 1022, 1085
BsmAI GTCTC 5 cut(s) 63, 239, 349, 543, 624
BsmFI GGGAC 1 cut(s) 124
BsmI GAATGC 2 cut(s) 98, 987
BsnI GGCC 4 cut(s) 5, 131, 569, 1089
Bso31I GGTCTC 3 cut(s) 239, 349, 624
Bsp1286I GDGCHC 3 cut(s) 781, 1132, 1195
Bsp143I GATC 2 cut(s) 1003, 1141
Bsp1720I GCTNAGC 1 cut(s) 112
BspANI GGCC 4 cut(s) 5, 131, 569, 1089
BspCNI CTCAG 3 cut(s) 125, 198, 535
BspFNI CGCG 4 cut(s) 161, 163, 165, 297
BspLI GGNNCC 3 cut(s) 290, 325, 1192
BspMI ACCTGC 1 cut(s) 1097
BspPI GGATC 2 cut(s) 998, 1136
BspT107I GGYRCC 1 cut(s) 323
BspTNI GGTCTC 3 cut(s) 239, 349, 624
BsrDI GCAATG 2 cut(s) 717, 977
BsrI ACTGG 4 cut(s) 348, 412, 942, 1145
BssECI CCNNGG 5 cut(s) 15, 16, 151, 490, 1133
BssHII GCGCGC 2 cut(s) 159, 161
BssMI GATC 2 cut(s) 1003, 1141
BssNI GRCGYC 2 cut(s) 282, 324
BssSI CACGAG 2 cut(s) 894, 1032
BssT1I CCWWGG 1 cut(s) 1133
Bst2BI CACGAG 2 cut(s) 894, 1032
Bst2UI CCWGG 2 cut(s) 17, 399
Bst4CI ACNGT 1 cut(s) 1061
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 2 cut(s) 282, 324
BstBAI YACGTR 2 cut(s) 55, 241
BstC8I GCNNGC 3 cut(s) 161, 163, 804
BstDEI CTNAG 4 cut(s) 112, 185, 522, 861
BstDSI CCRYGG 1 cut(s) 490
BstF5I GGATG 3 cut(s) 11, 92, 970
BstFNI CGCG 4 cut(s) 161, 163, 165, 297
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 4 cut(s) 161, 163, 165, 326
BstKTI GATC 2 cut(s) 1006, 1144
BstMAI GTCTC 5 cut(s) 63, 239, 349, 543, 624
BstMBI GATC 2 cut(s) 1003, 1141
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 4 cut(s) 95, 162, 300, 857
BstNI CCWGG 2 cut(s) 17, 399
BstNSI RCATGY 4 cut(s) 442, 556, 672, 806
BstSCI CCNGG 2 cut(s) 15, 397
BstSFI CTRYAG 2 cut(s) 265, 918
BstUI CGCG 4 cut(s) 161, 163, 165, 297
BstV1I GCAGC 1 cut(s) 838
BstV2I GAAGAC 1 cut(s) 825
BsuI GTATCC 1 cut(s) 597
BsuRI GGCC 4 cut(s) 5, 131, 569, 1089
BtgI CCRYGG 1 cut(s) 490
BtsCI GGATG 3 cut(s) 11, 92, 970
BtsIMutI CAGTG 2 cut(s) 191, 355
BveI ACCTGC 1 cut(s) 1097
Cac8I GCNNGC 3 cut(s) 161, 163, 804
CaiI CAGNNNCTG 2 cut(s) 1112, 1171
CfoI GCGC 4 cut(s) 161, 163, 165, 326
Cfr13I GGNCC 3 cut(s) 130, 568, 1109
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviAII CATG 7 cut(s) 439, 553, 572, 669, 803, 823, 1153
DdeI CTNAG 4 cut(s) 112, 185, 522, 861
DinI GGCGCC 1 cut(s) 325
DpnI GATC 2 cut(s) 1005, 1143
DpnII GATC 2 cut(s) 1003, 1141
EaeI YGGCCR 2 cut(s) 3, 1087
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
EciI GGCGGA 2 cut(s) 38, 281
Ecl136II GAGCTC 1 cut(s) 1130
Eco130I CCWWGG 1 cut(s) 1133
Eco24I GRGCYC 2 cut(s) 1132, 1195
Eco31I GGTCTC 3 cut(s) 239, 349, 624
Eco32I GATATC 1 cut(s) 1042
Eco47I GGWCC 1 cut(s) 1109
Eco53kI GAGCTC 1 cut(s) 1130
Eco57I CTGAAG 2 cut(s) 1134, 1148
Eco72I CACGTG 2 cut(s) 55, 241
EcoICRI GAGCTC 1 cut(s) 1130
EcoO109I RGGNCCY 1 cut(s) 1109
EcoRI GAATTC 1 cut(s) 1026
EcoRII CCWGG 2 cut(s) 15, 397
EcoRV GATATC 1 cut(s) 1042
EcoT14I CCWWGG 1 cut(s) 1133
EcoT22I ATGCAT 1 cut(s) 670
EcoT38I GRGCYC 2 cut(s) 1132, 1195
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 1133
FaeI CATG 7 cut(s) 442, 556, 575, 672, 806, 826, 1156
FaqI GGGAC 1 cut(s) 124
FatI CATG 7 cut(s) 438, 552, 571, 668, 802, 822, 1152
FauI CCCGC 3 cut(s) 187, 462, 988
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 5 cut(s) 87, 117, 157, 295, 852
FokI GGATG 2 cut(s) 79, 977
FriOI GRGCYC 2 cut(s) 1132, 1195
Fsp4HI GCNGC 5 cut(s) 87, 117, 157, 295, 852
FspBI CTAG 1 cut(s) 1094
GlaI GCGC 4 cut(s) 160, 162, 164, 325
GluI GCNGC 5 cut(s) 87, 117, 157, 295, 852
GsuI CTGGAG 4 cut(s) 331, 429, 1193, 1209
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 4 cut(s) 5, 131, 569, 1089
HhaI GCGC 4 cut(s) 161, 163, 165, 326
Hin1I GRCGYC 2 cut(s) 282, 324
Hin1II CATG 7 cut(s) 442, 556, 575, 672, 806, 826, 1156
Hin6I GCGC 4 cut(s) 159, 161, 163, 324
HinP1I GCGC 4 cut(s) 159, 161, 163, 324
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 4 cut(s) 274, 374, 422, 659
HphI GGTGA 2 cut(s) 53, 842
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 6 cut(s) 308, 591, 664, 1127, 1213, 1234
Hpy188III TCNNGA 8 cut(s) 278, 371, 656, 796, 1034, 1112, 1172, 1188
Hpy8I GTNNAC 1 cut(s) 273
Hpy99I CGWCG 3 cut(s) 284, 323, 1230
HpyAV CCTTC 2 cut(s) 185, 1172
HpyCH4III ACNGT 1 cut(s) 1061
HpyCH4IV ACGT 5 cut(s) 54, 105, 240, 282, 1228
HpyCH4V TGCA 7 cut(s) 668, 722, 773, 972, 987, 1106, 1243
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 162, 300, 857
HpyF3I CTNAG 4 cut(s) 112, 185, 522, 861
HpySE526I ACGT 5 cut(s) 54, 105, 240, 282, 1228
Hsp92I GRCGYC 2 cut(s) 282, 324
Hsp92II CATG 7 cut(s) 442, 556, 575, 672, 806, 826, 1156
HspAI GCGC 4 cut(s) 159, 161, 163, 324
KasI GGCGCC 1 cut(s) 323
Kzo9I GATC 2 cut(s) 1003, 1141
LmnI GCTCC 5 cut(s) 294, 1123, 1127, 1135, 1190
Lsp1109I GCAGC 1 cut(s) 838
LweI GCATC 3 cut(s) 20, 655, 1177
MaeI CTAG 1 cut(s) 1094
MaeII ACGT 5 cut(s) 54, 105, 240, 282, 1228
MaeIII GTNAC 3 cut(s) 350, 511, 1072
MalI GATC 2 cut(s) 1005, 1143
MauBI CGCGCGCG 1 cut(s) 161
MboI GATC 2 cut(s) 1003, 1141
MboII GAAGA 3 cut(s) 163, 830, 1049
MfeI CAATTG 2 cut(s) 1008, 1247
MhlI GDGCHC 3 cut(s) 781, 1132, 1195
MlsI TGGCCA 2 cut(s) 5, 1089
MluCI AATT 7 cut(s) 709, 789, 937, 952, 1008, 1026, 1247
MluNI TGGCCA 2 cut(s) 5, 1089
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 3 cut(s) 268, 368, 416
Mox20I TGGCCA 2 cut(s) 5, 1089
Mph1103I ATGCAT 1 cut(s) 670
MscI TGGCCA 2 cut(s) 5, 1089
MseI TTAA 4 cut(s) 404, 747, 837, 1206
Msp20I TGGCCA 2 cut(s) 5, 1089
MspR9I CCNGG 2 cut(s) 17, 399
MunI CAATTG 2 cut(s) 1008, 1247
Mva1269I GAATGC 2 cut(s) 98, 987
MvaI CCWGG 2 cut(s) 17, 399
MvnI CGCG 4 cut(s) 161, 163, 165, 297
MwoI GCNNNNNNNGC 4 cut(s) 95, 162, 300, 857
NarI GGCGCC 1 cut(s) 324
NdeII GATC 2 cut(s) 1003, 1141
NlaIII CATG 7 cut(s) 442, 556, 575, 672, 806, 826, 1156
NlaIV GGNNCC 3 cut(s) 290, 325, 1192
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 350, 1072
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 4 cut(s) 442, 556, 672, 806
PaeI GCATGC 1 cut(s) 806
PasI CCCWGGG 1 cut(s) 16
PauI GCGCGC 2 cut(s) 159, 161
PciI ACATGT 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PctI GAATGC 2 cut(s) 98, 987
PfeI GAWTC 1 cut(s) 659
PkrI GCNGC 5 cut(s) 88, 118, 158, 296, 853
PleI GAGTC 3 cut(s) 268, 368, 416
PluTI GGCGCC 1 cut(s) 327
PmaCI CACGTG 2 cut(s) 55, 241
PmlI CACGTG 2 cut(s) 55, 241
PpsI GAGTC 3 cut(s) 268, 368, 416
Ppu21I YACGTR 2 cut(s) 55, 241
PpuMI RGGWCCY 1 cut(s) 1109
PscI ACATGT 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 1132
Psp5II RGGWCCY 1 cut(s) 1109
Psp6I CCWGG 2 cut(s) 15, 397
PspCI CACGTG 2 cut(s) 55, 241
PspGI CCWGG 2 cut(s) 15, 397
PspN4I GGNNCC 3 cut(s) 290, 325, 1192
PspPI GGNCC 3 cut(s) 130, 568, 1109
PspPPI RGGWCCY 1 cut(s) 1109
PstNI CAGNNNCTG 2 cut(s) 1112, 1171
PteI GCGCGC 2 cut(s) 159, 161
SacI GAGCTC 1 cut(s) 1132
SalI GTCGAC 1 cut(s) 271
SaqAI TTAA 4 cut(s) 404, 747, 837, 1206
SatI GCNGC 5 cut(s) 87, 117, 157, 295, 852
Sau3AI GATC 2 cut(s) 1003, 1141
Sau96I GGNCC 3 cut(s) 130, 568, 1109
SchI GAGTC 3 cut(s) 268, 368, 416
ScrFI CCNGG 2 cut(s) 17, 399
SduI GDGCHC 3 cut(s) 781, 1132, 1195
SfaNI GCATC 3 cut(s) 20, 655, 1177
SfcI CTRYAG 2 cut(s) 265, 918
SfoI GGCGCC 1 cut(s) 325
SinI GGWCC 1 cut(s) 1109
SphI GCATGC 1 cut(s) 806
Sse9I AATT 7 cut(s) 709, 789, 937, 952, 1008, 1026, 1247
SspDI GGCGCC 1 cut(s) 323
SspI AATATT 1 cut(s) 751
SspMI CTAG 1 cut(s) 1094
SstI GAGCTC 1 cut(s) 1132
StyD4I CCNGG 2 cut(s) 15, 397
StyI CCWWGG 1 cut(s) 1133
TaaI ACNGT 1 cut(s) 1061
TaiI ACGT 5 cut(s) 57, 108, 243, 285, 1231
TaqI TCGA 5 cut(s) 144, 230, 272, 336, 448
TasI AATT 7 cut(s) 709, 789, 937, 952, 1008, 1026, 1247
TauI GCSGC 4 cut(s) 89, 119, 159, 297
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 4 cut(s) 404, 747, 837, 1206
Tru9I TTAA 4 cut(s) 404, 747, 837, 1206
TscAI CASTG 2 cut(s) 198, 355
TseFI GTSAC 2 cut(s) 350, 1072
TseI GCWGC 1 cut(s) 851
Tsp45I GTSAC 2 cut(s) 350, 1072
TspDTI ATGAA 2 cut(s) 528, 891
TspGWI ACGGA 1 cut(s) 725
TspRI CASTG 2 cut(s) 198, 355
VpaK11BI GGWCC 1 cut(s) 1109
XapI RAATTY 2 cut(s) 789, 1026
XceI RCATGY 4 cut(s) 442, 556, 672, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 272
XspI CTAG 1 cut(s) 1094
ZraI GACGTC 1 cut(s) 283
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.