Prupe.5G170300_v2.0.a1

Belongs to the GDA1 CD39 NTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0012614
Physical Location & Seq
Reverse (-)
14585135 .. 14588354
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G170300.1

Sequence Viewer

Length: 1638 bp
ATGGAACCCAAATCTCCTTCAAAACTTAAGCTCTCAATCATGGGATTTGCTCAATACAAGCAGGCGTTGAGAACGGGTTTTATGGTTACAGTTATAATACTCTTATTATTTGGTGCCTATTTTGTGTTTATACCCGGGAAAGGCCATAGTGTATCTAAAGGACCATATTATACGGTGGTTGTTGATTGTGGAAGCACAGGGACCCGAGTAAATGTGTACAAGTGGCTGGCAAGGGCTGTAAGTAGTAAGGAATTGCCGATTTTGTTGTATTCTTATCCCGACAATTCAACTGATGGAATGCTTTGGAAAAGTTGTAAGTATCACTGTCTGCAAACTGAGCCTGGTTTGGATAAATCTGTCGGAAACTTATCAGGAGTAAGAGCATCTTTGGAACCATTAATTACATTGGCAGAACATAAGGTTCCTTCTGAAAAACGCAGAGACACTCCCATTTTTGTTCTAGCTACTGCTGGACTAAGGAGATTGGCAATGGAGGATGCTAGGCGGGTATTGAATGACATAGAAGCTATTGTAAAAGAACATACTTTTTTGTATGAAAAGAGTTGGATACGGGTGTTGAGTGGCCAAGAAGAAGCTTATTATGGTTGGGTGGCTCTAAATTATAAAATGGGTAGCTTTGGGAATCATTCAAGGTCACCCACTTTGGGACTTCTTGACTTAGGAGGTTCATCATTGCAGATTGTCGTGGAAGTTGATGATACAAGAGAAGATGCGAACTTAGTGAGATCAAAATTTGGTTTTGTCGAACATGATATTTTAGCTTACTCATTGTCGGAATTTGGATTGAATGAAGCATTTGATAGGACGGTTGTTATGCTCAGCCATATGGAACAACTCAGAGAAAGTGCTTCTGGTATAGTAGAGATCAGACATCCTTGTCTTCATACTGACATTGTGCAAAACTATACCTGCTATGGTTGCTCCCAGCTTAACGCTCCTGATCAGAAAAAAGTGACCAGTCAAGTGCAGGAAACTAAATTTCCTTCTGTGTATCTGGTTGGAGTGCCGAATTGGGAGCAATGCAGAAGACTTGCTAGGGTTGCTGCTATCAATTCAAGCACAGCTGACACAGACCATAGAGCACGGTCATGTTCTGATAATGGGAGTGAAATGATAAATTTGACTGCTTTTGCTCACCCCAAAGCCCGCTTTCATGCACTTTCTGGATTTTTTGCTGTTTATGACAAGTTGAATTTGAGCACAAGAGCCACCTTGACGAAGATTTGGGAGAAAGGCCAGCAAGTATGCTCCAGATCAGGGTCTGACCTAAGCAGTAATTCACAGAATGCATATTTTACTTGGCAATATTGTTTTCGTGTGCCTTATGTGGCATCGCTCATTGACGATGCCCTGTGTCTTGGTGACAAAGAGATCATTTTTGGTCCTGCGGATGTTACTTGGACATTAGGAGCTGCATTGGTCGAAGGAGAATATTTATGGTCAAGTCCTACTAGATCTCAAAGTAGCATTTTAGCCCTAAATATGGAGGTTATATCCTCTCCCATTTTTGTACTTGTTTTACTTCTTTGTATTCTATTGATTGTATATTGTTGTCAAGTCAAGCTCCCCATGATAGGCAGGAAGAGCACAGTAAGAGGACAGAAGATGATAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000139 GO:0003006 GO:0003674 GO:0003824 GO:0004382 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006139 GO:0006152 GO:0006163 GO:0006195 GO:0006213 GO:0006220 GO:0006244 GO:0006256 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007548 GO:0008150 GO:0008152 GO:0008406 GO:0009056 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009125 GO:0009126 GO:0009128 GO:0009132 GO:0009134 GO:0009135 GO:0009137 GO:0009138 GO:0009140 GO:0009150 GO:0009154 GO:0009158 GO:0009161 GO:0009164 GO:0009166 GO:0009167 GO:0009169 GO:0009179 GO:0009181 GO:0009185 GO:0009191 GO:0009193 GO:0009195 GO:0009218 GO:0009222 GO:0009259 GO:0009261 GO:0009653 GO:0009887 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0012505 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017110 GO:0018130 GO:0019222 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0022414 GO:0030173 GO:0030334 GO:0031090 GO:0031224 GO:0031228 GO:0031300 GO:0031301 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031984 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032879 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0034656 GO:0035262 GO:0040012 GO:0042278 GO:0042454 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043262 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045134 GO:0045137 GO:0046031 GO:0046032 GO:0046048 GO:0046128 GO:0046130 GO:0046131 GO:0046133 GO:0046135 GO:0046434 GO:0046483 GO:0046700 GO:0046710 GO:0046712 GO:0048513 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051270 GO:0055086 GO:0060049 GO:0060050 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072521 GO:0072523 GO:0072527 GO:0072529 GO:0080090 GO:0098588 GO:0098791 GO:1901068 GO:1901069 GO:1901135 GO:1901136 GO:1901292 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901657 GO:1901658 GO:1903018 GO:1903020 GO:2000112 GO:2000145
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko05169 map00230 map00240 map05169
Pfam Domains
Protein Families
GDA1_CD39

Protein Analysis

546

Amino Acids

60.77

Weight (kDa)

8.27

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 95, 624
AasI GACNNNNNNGTC 1 cut(s) 897
Acc36I ACCTGC 1 cut(s) 938
AccB1I GGYRCC 1 cut(s) 113
AciI CCGC 3 cut(s) 505, 1168, 1409
AcoI YGGCCR 1 cut(s) 583
AcsI RAATTY 5 cut(s) 752, 797, 998, 1138, 1213
AfaI GTAC 2 cut(s) 218, 1533
AfiI CCNNNNNNNGG 5 cut(s) 140, 665, 1278, 1504, 1595
AflII CTTAAG 1 cut(s) 26
AgsI TTSAA 7 cut(s) 21, 288, 514, 651, 808, 1077, 1213
AjnI CCWGG 1 cut(s) 340
Alw21I GWGCWC 3 cut(s) 1105, 1223, 1610
Alw26I GTCTC 1 cut(s) 435
AlwNI CAGNNNCTG 1 cut(s) 1283
Ama87I CYCGRG 2 cut(s) 134, 204
AoxI GGCC 3 cut(s) 142, 583, 1255
ApeKI GCWGC 2 cut(s) 1064, 1433
ApoI RAATTY 5 cut(s) 752, 797, 998, 1138, 1213
AseI ATTAAT 1 cut(s) 398
AspS9I GGNCC 3 cut(s) 161, 201, 1403
AsuC2I CCSGG 2 cut(s) 135, 136
AsuHPI GGTGA 3 cut(s) 648, 1148, 1394
AvaI CYCGRG 2 cut(s) 134, 204
AvaII GGWCC 3 cut(s) 161, 201, 1403
BaeI ACNNNNGTAYC 2 cut(s) 560, 593
BalI TGGCCA 1 cut(s) 585
BanI GGYRCC 1 cut(s) 113
BbsI GAAGAC 2 cut(s) 893, 1054
Bbv12I GWGCWC 3 cut(s) 1105, 1223, 1610
BbvI GCAGC 2 cut(s) 1051, 1420
BccI CCATC 1 cut(s) 287
BciT130I CCWGG 1 cut(s) 342
BciVI GTATCC 1 cut(s) 561
BclI TGATCA 1 cut(s) 961
BcnI CCSGG 2 cut(s) 135, 136
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 4 cut(s) 461, 501, 1056, 1473
BfrI CTTAAG 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 938
BfuI GTATCC 1 cut(s) 561
BglII AGATCT 1 cut(s) 1475
BisI GCNGC 2 cut(s) 1065, 1434
BlpI GCTNAGC 1 cut(s) 839
BlsI GCNGC 2 cut(s) 1066, 1435
Bme1390I CCNGG 3 cut(s) 135, 136, 342
Bme18I GGWCC 3 cut(s) 161, 201, 1403
BmeT110I CYCGRG 2 cut(s) 134, 204
BmgT120I GGNCC 3 cut(s) 161, 201, 1403
BmiI GGNNCC 6 cut(s) 6, 115, 202, 203, 393, 423
BmrFI CCNGG 3 cut(s) 135, 136, 342
BmsI GCATC 5 cut(s) 392, 487, 721, 1357, 1361
BpiI GAAGAC 2 cut(s) 893, 1054
BpmI CTGGAG 1 cut(s) 1255
Bpu10I CCTNAGC 1 cut(s) 1289
Bpu1102I GCTNAGC 1 cut(s) 839
BpuMI CCSGG 2 cut(s) 135, 136
BsaJI CCNNGG 1 cut(s) 134
Bsc4I CCNNNNNNNGG 5 cut(s) 140, 665, 1278, 1504, 1595
Bse1I ACTGG 1 cut(s) 978
Bse3DI GCAATG 3 cut(s) 495, 692, 1046
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 3 cut(s) 502, 892, 1417
BseLI CCNNNNNNNGG 5 cut(s) 140, 665, 1278, 1504, 1595
BseMI GCAATG 3 cut(s) 495, 692, 1046
BseMII CTCAG 3 cut(s) 327, 853, 871
BseNI ACTGG 1 cut(s) 978
BseXI GCAGC 2 cut(s) 1051, 1420
BseYI CCCAGC 1 cut(s) 945
BsgI GTGCAG 1 cut(s) 1007
BshFI GGCC 3 cut(s) 144, 585, 1257
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 3 cut(s) 1105, 1223, 1610
BsiHKCI CYCGRG 2 cut(s) 134, 204
BsiSI CCGG 1 cut(s) 135
BslFI GGGAC 2 cut(s) 214, 681
BslI CCNNNNNNNGG 5 cut(s) 140, 665, 1278, 1504, 1595
BsmAI GTCTC 1 cut(s) 435
BsmFI GGGAC 2 cut(s) 214, 681
BsmI GAATGC 2 cut(s) 303, 1312
BsnI GGCC 3 cut(s) 144, 585, 1257
BsoBI CYCGRG 2 cut(s) 134, 204
Bsp1286I GDGCHC 3 cut(s) 1105, 1223, 1610
Bsp1407I TGTACA 1 cut(s) 216
Bsp143I GATC 6 cut(s) 746, 885, 961, 1274, 1392, 1475
Bsp1720I GCTNAGC 1 cut(s) 839
BspACI CCGC 3 cut(s) 505, 1168, 1409
BspANI GGCC 3 cut(s) 144, 585, 1257
BspCNI CTCAG 3 cut(s) 328, 852, 870
BspLI GGNNCC 6 cut(s) 6, 115, 202, 203, 393, 423
BspMI ACCTGC 1 cut(s) 938
BspQI GCTCTTC 1 cut(s) 1598
BspT107I GGYRCC 1 cut(s) 113
BspTI CTTAAG 1 cut(s) 26
BsrDI GCAATG 3 cut(s) 495, 692, 1046
BsrGI TGTACA 1 cut(s) 216
BsrI ACTGG 1 cut(s) 978
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 6 cut(s) 746, 885, 961, 1274, 1392, 1475
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 6 cut(s) 91, 175, 326, 829, 1107, 1612
Bst6I CTCTTC 1 cut(s) 1598
BstAFI CTTAAG 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 216
BstC8I GCNNGC 4 cut(s) 63, 228, 1168, 1259
BstDEI CTNAG 7 cut(s) 336, 476, 679, 739, 839, 857, 1289
BstEII GGTNACC 1 cut(s) 654
BstF5I GGATG 3 cut(s) 502, 892, 1417
BstKTI GATC 6 cut(s) 749, 888, 964, 1277, 1395, 1478
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 6 cut(s) 746, 885, 961, 1274, 1392, 1475
BstMWI GCNNNNNNNGC 4 cut(s) 337, 939, 1061, 1605
BstNI CCWGG 1 cut(s) 342
BstPI GGTNACC 1 cut(s) 654
BstSCI CCNGG 3 cut(s) 133, 134, 340
BstV1I GCAGC 2 cut(s) 1051, 1420
BstV2I GAAGAC 2 cut(s) 893, 1054
BstX2I RGATCY 1 cut(s) 1475
BstYI RGATCY 1 cut(s) 1475
BsuI GTATCC 1 cut(s) 561
BsuRI GGCC 3 cut(s) 144, 585, 1257
BtgZI GCGATG 1 cut(s) 1338
BtsCI GGATG 3 cut(s) 502, 892, 1417
BtsIMutI CAGTG 1 cut(s) 322
BveI ACCTGC 1 cut(s) 938
Cac8I GCNNGC 4 cut(s) 63, 228, 1168, 1259
CaiI CAGNNNCTG 1 cut(s) 1283
Cfr13I GGNCC 3 cut(s) 161, 201, 1403
Cfr9I CCCGGG 1 cut(s) 134
Csp6I GTAC 2 cut(s) 217, 1532
CviAII CATG 5 cut(s) 40, 770, 1110, 1175, 1591
CviQI GTAC 2 cut(s) 217, 1532
DdeI CTNAG 7 cut(s) 336, 476, 679, 739, 839, 857, 1289
DpnI GATC 6 cut(s) 748, 887, 963, 1276, 1394, 1477
DpnII GATC 6 cut(s) 746, 885, 961, 1274, 1392, 1475
DrdI GACNNNNNNGTC 1 cut(s) 897
DseDI GACNNNNNNGTC 1 cut(s) 897
EaeI YGGCCR 1 cut(s) 583
Eam1104I CTCTTC 1 cut(s) 1598
EarI CTCTTC 1 cut(s) 1598
Eco47I GGWCC 3 cut(s) 161, 201, 1403
Eco88I CYCGRG 2 cut(s) 134, 204
Eco91I GGTNACC 1 cut(s) 654
EcoO109I RGGNCCY 1 cut(s) 201
EcoO65I GGTNACC 1 cut(s) 654
EcoRII CCWGG 1 cut(s) 340
EcoT22I ATGCAT 1 cut(s) 1312
FaeI CATG 5 cut(s) 43, 773, 1113, 1178, 1594
FalI AAGNNNNNCTT 2 cut(s) 370, 402
FaqI GGGAC 2 cut(s) 214, 681
FatI CATG 5 cut(s) 39, 769, 1109, 1174, 1590
FauI CCCGC 2 cut(s) 498, 1175
FauNDI CATATG 1 cut(s) 846
FbaI TGATCA 1 cut(s) 961
Fnu4HI GCNGC 2 cut(s) 1065, 1434
FokI GGATG 3 cut(s) 509, 879, 1424
Fsp4HI GCNGC 2 cut(s) 1065, 1434
FspBI CTAG 4 cut(s) 461, 501, 1056, 1473
GluI GCNGC 2 cut(s) 1065, 1434
GsaI CCCAGC 1 cut(s) 949
GsuI CTGGAG 1 cut(s) 1255
HaeIII GGCC 3 cut(s) 144, 585, 1257
HapII CCGG 1 cut(s) 135
Hin1II CATG 5 cut(s) 43, 773, 1113, 1178, 1594
HindIII AAGCTT 1 cut(s) 594
HinfI GANTC 1 cut(s) 643
HpaII CCGG 1 cut(s) 135
HphI GGTGA 3 cut(s) 648, 1148, 1394
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 8 cut(s) 362, 430, 796, 860, 890, 966, 1117, 1285
Hpy188III TCNNGA 6 cut(s) 278, 372, 674, 959, 1185, 1272
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 4 cut(s) 27, 435, 1014, 1439
HpyCH4III ACNGT 6 cut(s) 91, 175, 326, 829, 1107, 1612
HpyCH4V TGCA 8 cut(s) 331, 697, 919, 988, 1044, 1178, 1310, 1436
HpyF10VI GCNNNNNNNGC 4 cut(s) 337, 939, 1061, 1605
HpyF3I CTNAG 7 cut(s) 336, 476, 679, 739, 839, 857, 1289
Hsp92II CATG 5 cut(s) 43, 773, 1113, 1178, 1594
KflI GGGWCCC 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 961
Kzo9I GATC 6 cut(s) 746, 885, 961, 1274, 1392, 1475
LguI GCTCTTC 1 cut(s) 1598
LmnI GCTCC 6 cut(s) 947, 961, 1036, 1274, 1430, 1590
Lsp1109I GCAGC 2 cut(s) 1051, 1420
LweI GCATC 5 cut(s) 392, 487, 721, 1357, 1361
MaeI CTAG 4 cut(s) 461, 501, 1056, 1473
MaeIII GTNAC 5 cut(s) 85, 654, 973, 1382, 1414
MalI GATC 6 cut(s) 748, 887, 963, 1276, 1394, 1477
MboI GATC 6 cut(s) 746, 885, 961, 1274, 1392, 1475
MboII GAAGA 7 cut(s) 602, 740, 893, 1059, 1252, 1615, 1636
MflI RGATCY 1 cut(s) 1475
MhlI GDGCHC 3 cut(s) 1105, 1223, 1610
MlsI TGGCCA 1 cut(s) 585
MluNI TGGCCA 1 cut(s) 585
MmeI TCCRAC 4 cut(s) 340, 545, 774, 1000
MnlI CCTC 5 cut(s) 487, 677, 1501, 1528, 1610
Mox20I TGGCCA 1 cut(s) 585
Mph1103I ATGCAT 1 cut(s) 1312
MscI TGGCCA 1 cut(s) 585
MseI TTAA 3 cut(s) 27, 398, 951
MslI CAYNNNNRTG 1 cut(s) 1108
Msp20I TGGCCA 1 cut(s) 585
MspA1I CMGCKG 1 cut(s) 1085
MspCI CTTAAG 1 cut(s) 26
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 3 cut(s) 135, 136, 342
Mva1269I GAATGC 2 cut(s) 303, 1312
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 4 cut(s) 337, 939, 1061, 1605
NciI CCSGG 2 cut(s) 135, 136
NdeI CATATG 1 cut(s) 846
NdeII GATC 6 cut(s) 746, 885, 961, 1274, 1392, 1475
NlaIII CATG 5 cut(s) 43, 773, 1113, 1178, 1594
NlaIV GGNNCC 6 cut(s) 6, 115, 202, 203, 393, 423
NmuCI GTSAC 3 cut(s) 654, 973, 1382
NsiI ATGCAT 1 cut(s) 1312
PciSI GCTCTTC 1 cut(s) 1598
PctI GAATGC 2 cut(s) 303, 1312
PfeI GAWTC 1 cut(s) 643
PkrI GCNGC 2 cut(s) 1066, 1435
PpuMI RGGWCCY 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 398
PsiI TTATAA 2 cut(s) 95, 624
Psp5II RGGWCCY 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 340
PspEI GGTNACC 1 cut(s) 654
PspFI CCCAGC 1 cut(s) 945
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 6 cut(s) 6, 115, 202, 203, 393, 423
PspPI GGNCC 3 cut(s) 161, 201, 1403
PspPPI RGGWCCY 1 cut(s) 201
PstNI CAGNNNCTG 1 cut(s) 1283
PsuI RGATCY 1 cut(s) 1475
PvuII CAGCTG 1 cut(s) 1085
RsaI GTAC 2 cut(s) 218, 1533
RsaNI GTAC 2 cut(s) 217, 1532
RseI CAYNNNNRTG 1 cut(s) 1108
SapI GCTCTTC 1 cut(s) 1598
SaqAI TTAA 3 cut(s) 27, 398, 951
SatI GCNGC 2 cut(s) 1065, 1434
Sau3AI GATC 6 cut(s) 746, 885, 961, 1274, 1392, 1475
Sau96I GGNCC 3 cut(s) 161, 201, 1403
ScrFI CCNGG 3 cut(s) 135, 136, 342
SduI GDGCHC 3 cut(s) 1105, 1223, 1610
SfaNI GCATC 5 cut(s) 392, 487, 721, 1357, 1361
SinI GGWCC 3 cut(s) 161, 201, 1403
SmaI CCCGGG 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 1108
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SsiI CCGC 3 cut(s) 505, 1168, 1409
SspI AATATT 2 cut(s) 1328, 1454
SspMI CTAG 4 cut(s) 461, 501, 1056, 1473
StyD4I CCNGG 3 cut(s) 133, 134, 340
TaaI ACNGT 6 cut(s) 91, 175, 326, 829, 1107, 1612
TaqI TCGA 2 cut(s) 765, 1443
TatI WGTACW 2 cut(s) 216, 1531
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 3 cut(s) 27, 398, 951
Tru9I TTAA 3 cut(s) 27, 398, 951
TscAI CASTG 1 cut(s) 329
TseFI GTSAC 3 cut(s) 654, 973, 1382
TseI GCWGC 2 cut(s) 1064, 1433
Tsp45I GTSAC 3 cut(s) 654, 973, 1382
TspDTI ATGAA 5 cut(s) 570, 678, 825, 893, 1163
TspMI CCCGGG 1 cut(s) 134
TspRI CASTG 1 cut(s) 329
Vha464I CTTAAG 1 cut(s) 26
VpaK11BI GGWCC 3 cut(s) 161, 201, 1403
VspI ATTAAT 1 cut(s) 398
XapI RAATTY 5 cut(s) 752, 797, 998, 1138, 1213
XmaI CCCGGG 1 cut(s) 134
XspI CTAG 4 cut(s) 461, 501, 1056, 1473
Zsp2I ATGCAT 1 cut(s) 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.