Prupe.6G047000_v2.0.a1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0021225
Physical Location & Seq
Forward (+)
3381613 .. 3383331
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G047000.1

Sequence Viewer

Length: 987 bp
ATGGTGGTGGATCAAGTCGGATCGACGAAGATCGAACAAAATCAAATGGAATTGGATCGCTACCCAGCGATGAAGCGGATTCGAGAGGAGTATAGTAAGATTGAATCCAAACCCCCCGACGATGGAGTCGGATCGACGCAGATCGAACAGAATCAAATGGAATTGGATCGCTACCCGAAGATGAAGCGGATTCGTGGGATTGAATCCAAACCCTCCGATCGTGTCGGATCGACGCAGATCGGACAAAATGAAACGGAAATCGGTTTGCGGAAAACTACCATCTCAAACTGTCTGTCTCGGCTTGAAGAGGAGTACAGAGAGATTAAATCCAAGCCCTCCGACGATTTTGACTGCCGCATGCTCGAGCGGAACCCATATGAATGGCAATTTGGAATCAGAGGCGCTAGTGGCACTGAATTCGAGAATGGGATTTATCATGGGGTGCTCCGGTTTAGGAAGAATTACCCATTGAAGCCTCCTTCAATCTCGCTTTTGACGGAAAATGGTCGCTTCAAAACCAACACCAAGATCAGGATAAAGCGACTGAATGATTGGCAGCCATCATGGGAGGTGCGAGATGCTTTACATGCACTTATCGATGAAATGCCCACCTACCCAGATGGTTTATTGGGTTCAGTAGAATACAATAAGGAAGAAAGGCGTGATCTGGCCAGGAAATCTCGTGAAGCAGCCCCAAAATATGGCACTTCTGAACGTCAGAAGTTAATTAATGATATTCATAAATATCTGCTAAACAAGTCACCATCTGATCCTGTTACTCAACTTCCTCAACTGAGCCCCGCACAAACCTCCAACGGCACAGGCGGTGGCTATGTCGTCAACGTTAGTGGGAATATATTTCATGCTACCAATTCCAACGGGGTAGGAATTTTGGGTTCTATGAATGAGTTCTCCGACGAAGGCCACAAGCCCAAGAAGGTTAAACTTTCTTGGCCAGAACTTTTTTGGCGAAGAAAGCAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 ko05012 map04120 map04141 map05012
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

37.67

Weight (kDa)

9.06

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AccB7I CCANNNNNTGG 1 cut(s) 701
AccBSI CCGCTC 1 cut(s) 367
AciI CCGC 7 cut(s) 76, 187, 268, 355, 367, 801, 825
AclI AACGTT 1 cut(s) 843
AclWI GGATC 7 cut(s) 18, 28, 63, 139, 174, 235, 764
AcoI YGGCCR 2 cut(s) 669, 953
AcsI RAATTY 2 cut(s) 416, 888
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 3 cut(s) 122, 531, 701
AgsI TTSAA 6 cut(s) 104, 203, 305, 472, 483, 514
AjnI CCWGG 1 cut(s) 671
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 7 cut(s) 18, 28, 63, 139, 174, 235, 764
Ama87I CYCGRG 1 cut(s) 362
AoxI GGCC 3 cut(s) 669, 922, 953
ApeKI GCWGC 2 cut(s) 556, 689
ApoI RAATTY 2 cut(s) 416, 888
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AseI ATTAAT 1 cut(s) 729
Asp700I GAANNNNTTC 1 cut(s) 908
AspLEI GCGC 1 cut(s) 404
AsuHPI GGTGA 1 cut(s) 753
AvaI CYCGRG 1 cut(s) 362
BalI TGGCCA 2 cut(s) 671, 955
BanII GRGCYC 1 cut(s) 800
BauI CACGAG 1 cut(s) 681
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 2 cut(s) 568, 701
BccI CCATC 5 cut(s) 116, 287, 568, 614, 772
BceAI ACGGC 1 cut(s) 832
BcgI CGANNNNNNTGC 2 cut(s) 400, 434
BciT130I CCWGG 1 cut(s) 673
BcoDI GTCTC 1 cut(s) 300
BfaI CTAG 2 cut(s) 405, 985
BfoI RGCGCY 1 cut(s) 405
BisI GCNGC 3 cut(s) 355, 557, 690
BlsI GCNGC 3 cut(s) 356, 558, 691
Bme1390I CCNGG 1 cut(s) 673
BmeT110I CYCGRG 1 cut(s) 362
BmiI GGNNCC 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 673
BmsI GCATC 1 cut(s) 568
Bsa29I ATCGAT 1 cut(s) 597
BsaWI WCCGGW 1 cut(s) 447
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 531, 701
BseBI CCWGG 1 cut(s) 673
BseCI ATCGAT 1 cut(s) 597
BseLI CCNNNNNNNGG 3 cut(s) 122, 531, 701
BseMII CTCAG 1 cut(s) 785
BseRI GAGGAG 2 cut(s) 101, 323
BseXI GCAGC 2 cut(s) 568, 701
BseYI CCCAGC 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 3 cut(s) 671, 924, 955
BshVI ATCGAT 1 cut(s) 597
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 1 cut(s) 448
BslI CCNNNNNNNGG 3 cut(s) 122, 531, 701
BsmAI GTCTC 1 cut(s) 300
BsnI GGCC 3 cut(s) 671, 924, 955
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 2 cut(s) 447, 800
BspACI CCGC 7 cut(s) 76, 187, 268, 355, 367, 801, 825
BspANI GGCC 3 cut(s) 671, 924, 955
BspCNI CTCAG 1 cut(s) 786
BspDI ATCGAT 1 cut(s) 597
BspLI GGNNCC 1 cut(s) 371
BspPI GGATC 7 cut(s) 18, 28, 63, 139, 174, 235, 764
BsrBI CCGCTC 1 cut(s) 367
BssSI CACGAG 1 cut(s) 681
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 673
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 794
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 1 cut(s) 404
BstMAI GTCTC 1 cut(s) 300
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 3 cut(s) 408, 587, 976
BstNI CCWGG 1 cut(s) 673
BstNSI RCATGY 2 cut(s) 361, 590
BstSCI CCNGG 1 cut(s) 671
BstV1I GCAGC 2 cut(s) 568, 701
BstXI CCANNNNNNTGG 1 cut(s) 381
Bsu15I ATCGAT 1 cut(s) 597
BsuRI GGCC 3 cut(s) 671, 924, 955
BsuTUI ATCGAT 1 cut(s) 597
BtgZI GCGATG 1 cut(s) 83
BtsIMutI CAGTG 1 cut(s) 411
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 404
ClaI ATCGAT 1 cut(s) 597
CseI GACGC 2 cut(s) 145, 241
Csp6I GTAC 1 cut(s) 313
CspCI CAANNNNNGTGG 2 cut(s) 829, 864
CviAII CATG 5 cut(s) 358, 437, 564, 587, 863
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 1 cut(s) 794
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 2 cut(s) 669, 953
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 800
Eco88I CYCGRG 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 671
EcoT38I GRGCYC 1 cut(s) 800
FaeI CATG 5 cut(s) 361, 440, 567, 590, 866
FatI CATG 5 cut(s) 357, 436, 563, 586, 862
FauI CCCGC 1 cut(s) 808
FauNDI CATATG 1 cut(s) 376
Fnu4HI GCNGC 3 cut(s) 355, 557, 690
FriOI GRGCYC 1 cut(s) 800
Fsp4HI GCNGC 3 cut(s) 355, 557, 690
FspBI CTAG 2 cut(s) 405, 985
GlaI GCGC 1 cut(s) 403
GluI GCNGC 3 cut(s) 355, 557, 690
GsaI CCCAGC 1 cut(s) 68
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 3 cut(s) 671, 924, 955
HapII CCGG 1 cut(s) 448
HgaI GACGC 2 cut(s) 145, 241
HhaI GCGC 1 cut(s) 404
Hin1II CATG 5 cut(s) 361, 440, 567, 590, 866
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HinfI GANTC 7 cut(s) 79, 104, 126, 151, 190, 203, 393
HpaII CCGG 1 cut(s) 448
HphI GGTGA 1 cut(s) 753
Hpy166II GTNNAC 1 cut(s) 841
Hpy188III TCNNGA 4 cut(s) 83, 421, 532, 683
Hpy8I GTNNAC 1 cut(s) 841
Hpy99I CGWCG 6 cut(s) 28, 122, 139, 235, 344, 920
HpyAV CCTTC 3 cut(s) 489, 914, 931
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 2 cut(s) 715, 843
HpyCH4V TGCA 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 3 cut(s) 408, 587, 976
HpyF3I CTNAG 1 cut(s) 794
HpySE526I ACGT 2 cut(s) 715, 843
Hsp92II CATG 5 cut(s) 361, 440, 567, 590, 866
HspAI GCGC 1 cut(s) 402
LmnI GCTCC 1 cut(s) 450
Lsp1109I GCAGC 2 cut(s) 568, 701
LweI GCATC 1 cut(s) 568
MaeI CTAG 2 cut(s) 405, 985
MaeII ACGT 2 cut(s) 715, 843
MaeIII GTNAC 2 cut(s) 759, 775
MbiI CCGCTC 1 cut(s) 367
MboII GAAGA 6 cut(s) 40, 190, 317, 469, 665, 984
MhlI GDGCHC 2 cut(s) 447, 800
MlsI TGGCCA 2 cut(s) 671, 955
MluCI AATT 8 cut(s) 50, 161, 386, 416, 460, 726, 871, 888
MluNI TGGCCA 2 cut(s) 671, 955
MlyI GAGTC 1 cut(s) 135
MmeI TCCRAC 6 cut(s) 109, 205, 363, 837, 900, 939
MnlI CCTC 9 cut(s) 79, 223, 301, 346, 392, 486, 562, 798, 820
Mox20I TGGCCA 2 cut(s) 671, 955
MroXI GAANNNNTTC 1 cut(s) 908
MscI TGGCCA 2 cut(s) 671, 955
MseI TTAA 4 cut(s) 324, 725, 729, 942
MslI CAYNNNNRTG 1 cut(s) 379
Msp20I TGGCCA 2 cut(s) 671, 955
MspI CCGG 1 cut(s) 448
MspR9I CCNGG 1 cut(s) 673
MvaI CCWGG 1 cut(s) 673
MwoI GCNNNNNNNGC 3 cut(s) 408, 587, 976
NdeI CATATG 1 cut(s) 376
NlaIII CATG 5 cut(s) 361, 440, 567, 590, 866
NlaIV GGNNCC 1 cut(s) 371
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 759
NspI RCATGY 2 cut(s) 361, 590
PacI TTAATTAA 1 cut(s) 729
PaeI GCATGC 1 cut(s) 361
PaeR7I CTCGAG 1 cut(s) 362
PdmI GAANNNNTTC 1 cut(s) 908
PfeI GAWTC 6 cut(s) 79, 104, 151, 190, 203, 393
PflMI CCANNNNNTGG 1 cut(s) 701
PkrI GCNGC 3 cut(s) 356, 558, 691
Ple19I CGATCG 1 cut(s) 220
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
PshBI ATTAAT 1 cut(s) 729
Psp1406I AACGTT 1 cut(s) 843
Psp6I CCWGG 1 cut(s) 671
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 1 cut(s) 671
PspN4I GGNNCC 1 cut(s) 371
PspXI VCTCGAGB 1 cut(s) 362
PvuI CGATCG 1 cut(s) 220
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 379
SaqAI TTAA 4 cut(s) 324, 725, 729, 942
SatI GCNGC 3 cut(s) 355, 557, 690
SchI GAGTC 1 cut(s) 135
ScrFI CCNGG 1 cut(s) 673
SduI GDGCHC 2 cut(s) 447, 800
SetI ASST 6 cut(s) 573, 614, 718, 812, 846, 942
SfaNI GCATC 1 cut(s) 568
Sfr274I CTCGAG 1 cut(s) 362
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 379
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
SphI GCATGC 1 cut(s) 361
Sse9I AATT 8 cut(s) 50, 161, 386, 416, 460, 726, 871, 888
SsiI CCGC 7 cut(s) 76, 187, 268, 355, 367, 801, 825
SspMI CTAG 2 cut(s) 405, 985
StyD4I CCNGG 1 cut(s) 671
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 2 cut(s) 718, 846
TaqI TCGA 9 cut(s) 23, 33, 82, 134, 144, 230, 363, 420, 597
TasI AATT 8 cut(s) 50, 161, 386, 416, 460, 726, 871, 888
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 6 cut(s) 79, 104, 151, 190, 203, 393
Tru1I TTAA 4 cut(s) 324, 725, 729, 942
Tru9I TTAA 4 cut(s) 324, 725, 729, 942
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 1 cut(s) 759
TseI GCWGC 2 cut(s) 556, 689
Tsp45I GTSAC 1 cut(s) 759
TspDTI ATGAA 8 cut(s) 86, 197, 264, 393, 615, 728, 851, 917
TspGWI ACGGA 2 cut(s) 269, 512
TspRI CASTG 1 cut(s) 418
Van91I CCANNNNNTGG 1 cut(s) 701
VspI ATTAAT 1 cut(s) 729
XapI RAATTY 2 cut(s) 416, 888
XceI RCATGY 2 cut(s) 361, 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 963
XhoI CTCGAG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 908
XspI CTAG 2 cut(s) 405, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.