Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp07

Orthogroup ID

OG0022418

Physical Location & Seq

Genomic Coordinates Forward (+)

5636681 .. 5640334

Gene Structure 3654 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.7G030400.1_v2.0.a1.CDS.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGGTACCGACCCCTGGTATGTCACACAATGGGAACTCAAATATGATGGCTACTTCTTCTGTGGATGCTTCTATGAACACTACAAGAGGAAGTACTAGCATTGCACCTACCACAGTCAACACAGGGAACCTGTTGCCTGCTGGCCCCTTACATGGCGGTTCATTCAATAGATCTGACGTTACAAAATCTGCTTTTTTTGGTTCTATGTCTAATGGATATCAGCAATCTCCTGCCAGTTTCTCCATTGGTACTGGAGGAAACAAGTCATCAATGGGTGTGCAAAGAATTATCCGCCAAATGATTCCTACTCCTGGGTTTAATAGCAGTAGTAATCAGTCTTACATGAATTTGGAATATTCTAATAATGGGGGTGGGTTTTCTACTGTTGATTCTTCAATGGTAACACAGCCACAACAGCAAAAGCAGCATATTGGTGGTCAAATTAGCCGTATACTTCATAACGTTGGCAGCCAAATGGGCAGTGAAATAAGGTCTGGCAAGCAGCAGACATCTTATGGGTTGCCAAATGGGGCTCTAAATGGTGGGTTGGGATTGATTGGGAACAATTTACCACTGGTTAATGAATCTGGAACTTCTGAGGGCTATATGACATCAACATCTTGTGCTAACTCTTCCAAACCATCACAACAACACTGTGATCAACATCAGCGGCCTAGAATGCAAGTAAGTAGCTTAATTCTCAACAGTGATAGCTATGGAATGAGTAATGTTGATTCTTTTGGGCCTGGAAACTACTATGGTGCTGCAACGCCTGTTGGGTCATTGTTGAATGTTCAGAATTTGAATTCAGTCAGCTCAACACCTATATCCAAGACCAACTCTCCCCTTATAAGTAATCAGTCGAATATGCACGGTGCACAACAAAGTGTACATGTGAAGCCTCAACAACTTGATCAATTAGAAAAGATAAGTTTCCAAACTCCGTTATCTTCAAGAGAGAATATCCTCCATTCTCATCAACCTAATCAGTTTCAACAGCAGCAGCAATTATTTCACCAGCAACGTCAACAGAAGCAGCAGAATCCGCAGCCCCAGCAGCTGTTAAATAATGATGCTTTTGGTCAGTCTCAAATGACATCTGATCTCAACAGTGCAAAGCGTGACATGGACCACCATAATGAAGCTACGCACCAACAAGCTACAGAACCGTTTCGATTATCGGAGATGCATAACCAATTCCATCAGCACTCTGTTGAAGATCGTTTGAGGAATGCTCCGCATATCCCATCTGGTCAACATGATATCTCCTCTTCATTGTCTCGAACTTCTCAGCAAATGCAACAAATATTGCAGCCCCACCAATTGGTTGCAGGGTCTCAAAATGATTTCAGATCTCTCTCTGTCGGAGCTCAATCAGAACCAGAACTGCAAGGTCAATGGCATCCTCAATCGCAAGATGGGAGTCACAGACAGGCCAACATGTCCCATGAGCAGCATGTCCATGAAGATTTCCGTCAGAGGATATCTGGGCAAGATGAAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004402 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0007275 GO:0008080 GO:0008150 GO:0008152 GO:0009791 GO:0009908 GO:0009987 GO:0016043 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016740 GO:0016746 GO:0016747 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019538 GO:0022414 GO:0032501 GO:0032502 GO:0034212 GO:0036211 GO:0043170 GO:0043412 GO:0043543 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0051276 GO:0061458 GO:0061733 GO:0071704 GO:0071840 GO:0090567 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04024 ko04066 ko04068 ko04110 ko04310 ko04330 ko04350 ko04520 ko04630 ko04720 ko04916 ko04919 ko04922 ko05016 ko05152 ko05161 ko05164 ko05165 ko05166 ko05167 ko05168 ko05169 ko05200 ko05203 ko05206 ko05211 ko05215 map04024 map04066 map04068 map04110 map04310 map04330 map04350 map04520 map04630 map04720 map04916 map04919 map04922 map05016 map05152 map05161 map05164 map05165 map05166 map05167 map05168 map05169 map05200 map05203 map05206 map05211 map05215

Pfam Domains

Protein Families

HAT_KAT11 PHD ZZ zf-TAZ

Protein Analysis

502

Amino Acids

54.66

Weight (kDa)

6.8

Isoelectric Point (pI)

62.82

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022418)

Species	Orthologous Gene IDs
malus_domestica	MD17G1157100.v1.1
prunus_persica	Prupe.7G030400_v2.0.a1
rosa_laevigata	RLG00000020338
rosa_samantha	Rh2AG464600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	851
Acc65I	GGTACC	1 cut(s)	4
AccB1I	GGYRCC	1 cut(s)	4
AccB7I	CCANNNNNTGG	1 cut(s)	1324
AccI	GTMKAC	1 cut(s)	451
AciI	CCGC	5 cut(s)	156, 292, 670, 1046, 1238
AclI	AACGTT	1 cut(s)	462
AcsI	RAATTY	3 cut(s)	346, 799, 805
AfaI	GTAC	4 cut(s)	6, 94, 250, 891
AfiI	CCNNNNNNNGG	4 cut(s)	14, 152, 311, 1324
AflIII	ACRYGT	2 cut(s)	892, 1440
AgsI	TTSAA	7 cut(s)	166, 396, 790, 805, 954, 995, 1217
AjnI	CCWGG	3 cut(s)	13, 310, 745
AluBI	AGCT	8 cut(s)	693, 714, 816, 1060, 1145, 1160, 1370, 1502
AluI	AGCT	8 cut(s)	693, 714, 816, 1060, 1145, 1160, 1370, 1502
Alw21I	GWGCWC	2 cut(s)	880, 1372
Alw26I	GTCTC	3 cut(s)	1092, 1284, 1341
Alw44I	GTGCAC	1 cut(s)	876
AlwNI	CAGNNNCTG	1 cut(s)	1060
AoxI	GGCC	4 cut(s)	142, 671, 743, 1434
ApaLI	GTGCAC	1 cut(s)	876
ApoI	RAATTY	3 cut(s)	346, 799, 805
Asp700I	GAANNNNTTC	1 cut(s)	1170
Asp718I	GGTACC	1 cut(s)	4
AspS9I	GGNCC	3 cut(s)	143, 743, 1129
AsuHPI	GGTGA	1 cut(s)	1007
AvaII	GGWCC	1 cut(s)	1129
BaeGI	GKGCMC	1 cut(s)	880
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	2 cut(s)	535, 1372
Bbv12I	GWGCWC	2 cut(s)	880, 1372
BccI	CCATC	5 cut(s)	40, 649, 1209, 1255, 1412
BceAI	ACGGC	1 cut(s)	432
BciT130I	CCWGG	3 cut(s)	15, 312, 747
BclI	TGATCA	2 cut(s)	658, 913
BcoDI	GTCTC	3 cut(s)	1092, 1284, 1341
BfaI	CTAG	2 cut(s)	96, 675
BfmI	CTRYAG	1 cut(s)	1161
BglI	GCCNNNNNGGC	1 cut(s)	477
BglII	AGATCT	2 cut(s)	170, 1352
BmcAI	AGTACT	1 cut(s)	94
Bme1390I	CCNGG	3 cut(s)	15, 312, 747
Bme18I	GGWCC	1 cut(s)	1129
BmgT120I	GGNCC	3 cut(s)	143, 743, 1129
BmiI	GGNNCC	3 cut(s)	6, 128, 145
BmrFI	CCNGG	3 cut(s)	15, 312, 747
BmsI	GCATC	4 cut(s)	55, 1063, 1176, 1411
BplI	GAGNNNNNCTC	2 cut(s)	1219, 1251
BpmI	CTGGAG	1 cut(s)	273
BsaBI	GATNNNNATC	1 cut(s)	663
BsaI	GGTCTC	1 cut(s)	1341
BsaJI	CCNNGG	2 cut(s)	13, 311
BsaXI	ACNNNNNCTCC	2 cut(s)	826, 856
Bsc4I	CCNNNNNNNGG	4 cut(s)	14, 152, 311, 1324
Bse1I	ACTGG	3 cut(s)	234, 256, 579
Bse3DI	GCAATG	1 cut(s)	99
Bse8I	GATNNNNATC	1 cut(s)	663
BseBI	CCWGG	3 cut(s)	15, 312, 747
BseDI	CCNNGG	2 cut(s)	13, 311
BseGI	GGATG	2 cut(s)	70, 1402
BseJI	GATNNNNATC	1 cut(s)	663
BseLI	CCNNNNNNNGG	4 cut(s)	14, 152, 311, 1324
BseMI	GCAATG	1 cut(s)	99
BseMII	CTCAG	2 cut(s)	588, 1304
BseNI	ACTGG	3 cut(s)	234, 256, 579
BseRI	GAGGAG	1 cut(s)	1258
BseSI	GKGCMC	1 cut(s)	880
BseYI	CCCAGC	1 cut(s)	1053
BshFI	GGCC	4 cut(s)	144, 673, 745, 1436
BshNI	GGYRCC	1 cut(s)	4
BsiHKAI	GWGCWC	2 cut(s)	880, 1372
BslFI	GGGAC	1 cut(s)	1429
BslI	CCNNNNNNNGG	4 cut(s)	14, 152, 311, 1324
BsmAI	GTCTC	3 cut(s)	1092, 1284, 1341
BsmFI	GGGAC	1 cut(s)	1429
BsmI	GAATGC	2 cut(s)	684, 1237
BsnI	GGCC	4 cut(s)	144, 673, 745, 1436
Bso31I	GGTCTC	1 cut(s)	1341
Bsp1286I	GDGCHC	3 cut(s)	535, 880, 1372
Bsp1407I	TGTACA	1 cut(s)	889
Bsp143I	GATC	6 cut(s)	170, 658, 913, 1102, 1219, 1352
BspACI	CCGC	5 cut(s)	156, 292, 670, 1046, 1238
BspANI	GGCC	4 cut(s)	144, 673, 745, 1436
BspCNI	CTCAG	2 cut(s)	589, 1303
BspLI	GGNNCC	3 cut(s)	6, 128, 145
BspT107I	GGYRCC	1 cut(s)	4
BspTNI	GGTCTC	1 cut(s)	1341
BsrDI	GCAATG	1 cut(s)	99
BsrGI	TGTACA	1 cut(s)	889
BsrI	ACTGG	3 cut(s)	234, 256, 579
BssECI	CCNNGG	2 cut(s)	13, 311
BssMI	GATC	6 cut(s)	170, 658, 913, 1102, 1219, 1352
BssNAI	GTATAC	1 cut(s)	452
Bst1107I	GTATAC	1 cut(s)	452
Bst2UI	CCWGG	3 cut(s)	15, 312, 747
Bst4CI	ACNGT	7 cut(s)	115, 385, 656, 707, 875, 1112, 1170
Bst6I	CTCTTC	2 cut(s)	637, 1276
BstAUI	TGTACA	1 cut(s)	889
BstC8I	GCNNGC	3 cut(s)	138, 142, 500
BstDEI	CTNAG	3 cut(s)	597, 1290, 1503
BstF5I	GGATG	2 cut(s)	70, 1402
BstKTI	GATC	6 cut(s)	173, 661, 916, 1105, 1222, 1355
BstMAI	GTCTC	3 cut(s)	1092, 1284, 1341
BstMBI	GATC	6 cut(s)	170, 658, 913, 1102, 1219, 1352
BstMWI	GCNNNNNNNGC	7 cut(s)	415, 424, 477, 679, 1045, 1054, 1057
BstNI	CCWGG	3 cut(s)	15, 312, 747
BstNSI	RCATGY	3 cut(s)	896, 1444, 1460
BstSCI	CCNGG	3 cut(s)	13, 310, 745
BstSFI	CTRYAG	1 cut(s)	1161
BstSLI	GKGCMC	1 cut(s)	880
BstX2I	RGATCY	2 cut(s)	170, 1352
BstYI	RGATCY	2 cut(s)	170, 1352
BstZ17I	GTATAC	1 cut(s)	452
BsuRI	GGCC	4 cut(s)	144, 673, 745, 1436
BtsCI	GGATG	2 cut(s)	70, 1402
BtsI	GCAGTG	1 cut(s)	487
BtsIMutI	CAGTG	5 cut(s)	487, 572, 652, 712, 1117
Cac8I	GCNNGC	3 cut(s)	138, 142, 500
CaiI	CAGNNNCTG	1 cut(s)	1060
Cfr13I	GGNCC	3 cut(s)	143, 743, 1129
Csp6I	GTAC	4 cut(s)	5, 93, 249, 890
CviAII	CATG	9 cut(s)	152, 343, 893, 1126, 1259, 1441, 1448, 1457, 1463
CviQI	GTAC	4 cut(s)	5, 93, 249, 890
DdeI	CTNAG	3 cut(s)	597, 1290, 1503
DpnI	GATC	6 cut(s)	172, 660, 915, 1104, 1221, 1354
DpnII	GATC	6 cut(s)	170, 658, 913, 1102, 1219, 1352
Eam1104I	CTCTTC	2 cut(s)	637, 1276
EarI	CTCTTC	2 cut(s)	637, 1276
EciI	GGCGGA	1 cut(s)	281
Ecl136II	GAGCTC	1 cut(s)	1370
Eco24I	GRGCYC	2 cut(s)	535, 1372
Eco31I	GGTCTC	1 cut(s)	1341
Eco32I	GATATC	3 cut(s)	218, 1264, 1485
Eco47I	GGWCC	1 cut(s)	1129
Eco53kI	GAGCTC	1 cut(s)	1370
EcoICRI	GAGCTC	1 cut(s)	1370
EcoRI	GAATTC	1 cut(s)	805
EcoRII	CCWGG	3 cut(s)	13, 310, 745
EcoRV	GATATC	3 cut(s)	218, 1264, 1485
EcoT22I	ATGCAT	1 cut(s)	1191
EcoT38I	GRGCYC	2 cut(s)	535, 1372
FaeI	CATG	9 cut(s)	155, 346, 896, 1129, 1262, 1444, 1451, 1460, 1466
FaqI	GGGAC	1 cut(s)	1429
FatI	CATG	9 cut(s)	151, 342, 892, 1125, 1258, 1440, 1447, 1456, 1462
FbaI	TGATCA	2 cut(s)	658, 913
FblI	GTMKAC	1 cut(s)	451
FokI	GGATG	2 cut(s)	77, 1389
FriOI	GRGCYC	2 cut(s)	535, 1372
FspBI	CTAG	2 cut(s)	96, 675
GsaI	CCCAGC	1 cut(s)	1057
GsuI	CTGGAG	1 cut(s)	273
HaeIII	GGCC	4 cut(s)	144, 673, 745, 1436
Hin1II	CATG	9 cut(s)	155, 346, 896, 1129, 1262, 1444, 1451, 1460, 1466
HincII	GTYRAC	3 cut(s)	118, 1028, 1256
HindII	GTYRAC	3 cut(s)	118, 1028, 1256
HindIII	AAGCTT	1 cut(s)	1500
HinfI	GANTC	6 cut(s)	301, 389, 584, 734, 1042, 1423
HphI	GGTGA	1 cut(s)	1007
Hpy166II	GTNNAC	6 cut(s)	118, 452, 878, 890, 1028, 1256
Hpy188I	TCNGA	9 cut(s)	175, 598, 798, 1102, 1183, 1352, 1367, 1378, 1479
Hpy188III	TCNNGA	3 cut(s)	588, 954, 1281
Hpy8I	GTNNAC	6 cut(s)	118, 452, 878, 890, 1028, 1256
HpyCH4III	ACNGT	7 cut(s)	115, 385, 656, 707, 875, 1112, 1170
HpyCH4IV	ACGT	3 cut(s)	177, 462, 1024
HpyF10VI	GCNNNNNNNGC	7 cut(s)	415, 424, 477, 679, 1045, 1054, 1057
HpyF3I	CTNAG	3 cut(s)	597, 1290, 1503
HpySE526I	ACGT	3 cut(s)	177, 462, 1024
Hsp92II	CATG	9 cut(s)	155, 346, 896, 1129, 1262, 1444, 1451, 1460, 1466
KpnI	GGTACC	1 cut(s)	8
Ksp22I	TGATCA	2 cut(s)	658, 913
Kzo9I	GATC	6 cut(s)	170, 658, 913, 1102, 1219, 1352
LmnI	GCTCC	2 cut(s)	1240, 1367
LweI	GCATC	4 cut(s)	55, 1063, 1176, 1411
MaeI	CTAG	2 cut(s)	96, 675
MaeII	ACGT	3 cut(s)	177, 462, 1024
MaeIII	GTNAC	5 cut(s)	21, 178, 400, 1121, 1424
MalI	GATC	6 cut(s)	172, 660, 915, 1104, 1221, 1354
MboI	GATC	6 cut(s)	170, 658, 913, 1102, 1219, 1352
MboII	GAAGA	7 cut(s)	48, 384, 624, 942, 1229, 1263, 1478
MfeI	CAATTG	1 cut(s)	1322
MflI	RGATCY	2 cut(s)	170, 1352
MhlI	GDGCHC	3 cut(s)	535, 880, 1372
MlyI	GAGTC	1 cut(s)	1432
MmeI	TCCRAC	1 cut(s)	1345
MnlI	CCTC	9 cut(s)	80, 248, 592, 912, 977, 1221, 1279, 1416, 1473
Mph1103I	ATGCAT	1 cut(s)	1191
MroXI	GAANNNNTTC	1 cut(s)	1170
MseI	TTAA	4 cut(s)	318, 579, 695, 1064
MslI	CAYNNNNRTG	3 cut(s)	432, 1137, 1461
MspA1I	CMGCKG	2 cut(s)	670, 1060
MspR9I	CCNGG	3 cut(s)	15, 312, 747
MunI	CAATTG	1 cut(s)	1322
Mva1269I	GAATGC	2 cut(s)	684, 1237
MvaI	CCWGG	3 cut(s)	15, 312, 747
MwoI	GCNNNNNNNGC	7 cut(s)	415, 424, 477, 679, 1045, 1054, 1057
NdeII	GATC	6 cut(s)	170, 658, 913, 1102, 1219, 1352
NlaIII	CATG	9 cut(s)	155, 346, 896, 1129, 1262, 1444, 1451, 1460, 1466
NlaIV	GGNNCC	3 cut(s)	6, 128, 145
NmuCI	GTSAC	3 cut(s)	21, 1121, 1424
NsiI	ATGCAT	1 cut(s)	1191
NspI	RCATGY	3 cut(s)	896, 1444, 1460
PciI	ACATGT	2 cut(s)	892, 1440
PctI	GAATGC	2 cut(s)	684, 1237
PdmI	GAANNNNTTC	1 cut(s)	1170
PfeI	GAWTC	5 cut(s)	301, 389, 584, 734, 1042
PflMI	CCANNNNNTGG	1 cut(s)	1324
PleI	GAGTC	1 cut(s)	1431
PpsI	GAGTC	1 cut(s)	1431
PscI	ACATGT	2 cut(s)	892, 1440
PsiI	TTATAA	1 cut(s)	851
Psp124BI	GAGCTC	1 cut(s)	1372
Psp1406I	AACGTT	1 cut(s)	462
Psp6I	CCWGG	3 cut(s)	13, 310, 745
PspFI	CCCAGC	1 cut(s)	1053
PspGI	CCWGG	3 cut(s)	13, 310, 745
PspN4I	GGNNCC	3 cut(s)	6, 128, 145
PspPI	GGNCC	3 cut(s)	143, 743, 1129
PstNI	CAGNNNCTG	1 cut(s)	1060
PsuI	RGATCY	2 cut(s)	170, 1352
PvuII	CAGCTG	1 cut(s)	1060
RsaI	GTAC	4 cut(s)	6, 94, 250, 891
RsaNI	GTAC	4 cut(s)	5, 93, 249, 890
RseI	CAYNNNNRTG	3 cut(s)	432, 1137, 1461
SacI	GAGCTC	1 cut(s)	1372
SaqAI	TTAA	4 cut(s)	318, 579, 695, 1064
Sau3AI	GATC	6 cut(s)	170, 658, 913, 1102, 1219, 1352
Sau96I	GGNCC	3 cut(s)	143, 743, 1129
ScaI	AGTACT	1 cut(s)	94
SchI	GAGTC	1 cut(s)	1432
ScrFI	CCNGG	3 cut(s)	15, 312, 747
SduI	GDGCHC	3 cut(s)	535, 880, 1372
SfaNI	GCATC	4 cut(s)	55, 1063, 1176, 1411
SfcI	CTRYAG	1 cut(s)	1161
SinI	GGWCC	1 cut(s)	1129
SmiMI	CAYNNNNRTG	3 cut(s)	432, 1137, 1461
SsiI	CCGC	5 cut(s)	156, 292, 670, 1046, 1238
SspI	AATATT	2 cut(s)	356, 1308
SspMI	CTAG	2 cut(s)	96, 675
SstI	GAGCTC	1 cut(s)	1372
StyD4I	CCNGG	3 cut(s)	13, 310, 745
TaaI	ACNGT	7 cut(s)	115, 385, 656, 707, 875, 1112, 1170
TaiI	ACGT	3 cut(s)	180, 465, 1027
TaqI	TCGA	3 cut(s)	863, 1174, 1282
TatI	WGTACW	2 cut(s)	92, 889
TauI	GCSGC	1 cut(s)	673
TfiI	GAWTC	5 cut(s)	301, 389, 584, 734, 1042
Tru1I	TTAA	4 cut(s)	318, 579, 695, 1064
Tru9I	TTAA	4 cut(s)	318, 579, 695, 1064
TscAI	CASTG	5 cut(s)	487, 579, 659, 712, 1117
TseFI	GTSAC	3 cut(s)	21, 1121, 1424
Tsp45I	GTSAC	3 cut(s)	21, 1121, 1424
TspDTI	ATGAA	8 cut(s)	89, 150, 359, 446, 597, 1155, 1263, 1479
TspGWI	ACGGA	2 cut(s)	933, 1463
TspRI	CASTG	5 cut(s)	487, 579, 659, 712, 1117
Van91I	CCANNNNNTGG	1 cut(s)	1324
VneI	GTGCAC	1 cut(s)	876
VpaK11BI	GGWCC	1 cut(s)	1129
XapI	RAATTY	3 cut(s)	346, 799, 805
XceI	RCATGY	3 cut(s)	896, 1444, 1460
XmiI	GTMKAC	1 cut(s)	451
XmnI	GAANNNNTTC	1 cut(s)	1170
XspI	CTAG	2 cut(s)	96, 675
ZrmI	AGTACT	1 cut(s)	94
Zsp2I	ATGCAT	1 cut(s)	1191

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.